EIF2B4

Summary

Gene Symbol: EIF2B4
Description: eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Alias: EIF-2B, EIF2B, EIF2Bdelta, eIF-2B GDP-GTP exchange factor subunit delta, eukaryotic translation initiation factor 2B subunit 4 delta, translation initiation factor eIF-2B subunit delta, translation initiation factor eIF-2b delta subunit
Species: human

Top Publications

  1. ncbi A severe variant of childhood ataxia with central hypomyelination/vanishing white matter leukoencephalopathy related to EIF21B5 mutation
    A Fogli
    INSERM UMR 384 et Fédération de Génétique Humaine Auvergne, Faculte de Medecine, Clermont Ferrand, France
    Neurology 59:1966-8. 2002
  2. pmc Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation
    E Gomez
    Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee, United Kingdom
    Mol Cell Biol 20:3965-76. 2000
  3. pmc Ovarian failure related to eukaryotic initiation factor 2B mutations
    Anne Fogli
    INSERM Unité Mixte de Recherche 384, Faculte de Medecine, Clermont Ferrand, France
    Am J Hum Genet 72:1544-50. 2003
  4. ncbi Decreased guanine nucleotide exchange factor activity in eIF2B-mutated patients
    Anne Fogli
    INSERM UMR 384, Faculte de Medecine, 28 Place Henri Dunant, Clermont Ferrand, France
    Eur J Hum Genet 12:561-6. 2004
  5. ncbi Mutations in each of the five subunits of translation initiation factor eIF2B can cause leukoencephalopathy with vanishing white matter
    Marjo S van der Knaap
    Department of Child Neurology, Free University Medical Center, Amsterdam, The Netherlands
    Ann Neurol 51:264-70. 2002
  6. pmc eIF2B-related disorders: antenatal onset and involvement of multiple organs
    Marjo S van der Knaap
    Department of Child Neurology, Free University Medical Center, Amsterdam, The Netherlands
    Am J Hum Genet 73:1199-207. 2003
  7. pmc Possible mechanisms involved in the down-regulation of translation during transient global ischaemia in the rat brain
    C Martín De La Vega
    Departamento de Investigacion, Hospital Ramon y Cajal, Ctra Colmenar Km 9, 28034 Madrid, Spain
    Biochem J 357:819-26. 2001
  8. ncbi Mechanism of inhibition of peptide chain initiation by amino acid deprivation in perfused rat liver. Regulation involving inhibition of eukaryotic initiation factor 2 alpha phosphatase activity
    S R Kimball
    Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey 17033
    J Biol Chem 266:1969-76. 1991
  9. ncbi Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B
    Yoshimitsu Kakuta
    Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812 8581, Japan
    Biochem Biophys Res Commun 319:725-32. 2004
  10. pmc Inhibition of glycogen synthase kinase 3[beta] activity with lithium in vitro attenuates sepsis-induced changes in muscle protein turnover
    Stephen Bertsch
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA
    Shock 35:266-74. 2011

Research Grants

  1. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    Jeffrey Bonanno; Fiscal Year: 2002
  2. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
  3. Amino Acids and Muscle Protein Synthesis
    Robert Wolfe; Fiscal Year: 2006
  4. Cell biological analysis of CACH/VWM leukodystrophy
    Christoph Proschel; Fiscal Year: 2005
  5. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    THOMAS VARY; Fiscal Year: 2002
  6. REGULATION OF LIVER GROWTH AND FUNCTION
    Leonard Jefferson; Fiscal Year: 2004
  7. REGULATION OF LIVER GROWTH AND FUNCTION
    Leonard Jefferson; Fiscal Year: 1999
  8. REGULATION OF SKELETAL MUSCLE METABOLISM
    Leonard Jefferson; Fiscal Year: 1999
  9. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
  10. Cytokine Regulation of Muscle Protein Synthesis During Infection
    Charles Lang; Fiscal Year: 2009

Scientific Experts

Detail Information

Publications147 found, 100 shown here

  1. ncbi A severe variant of childhood ataxia with central hypomyelination/vanishing white matter leukoencephalopathy related to EIF21B5 mutation
    A Fogli
    INSERM UMR 384 et Fédération de Génétique Humaine Auvergne, Faculte de Medecine, Clermont Ferrand, France
    Neurology 59:1966-8. 2002
    ..leukoencephalopathy is related to mutations in all five genes of the eukaryotic translation initiation factor (eIF2B)...
  2. pmc Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation
    E Gomez
    Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee, United Kingdom
    Mol Cell Biol 20:3965-76. 2000
    Eukaryotic translation initiation factor 2B (eIF2B) is the guanine nucleotide exchange factor for protein synthesis initiation factor 2 (eIF2)...
  3. pmc Ovarian failure related to eukaryotic initiation factor 2B mutations
    Anne Fogli
    INSERM Unité Mixte de Recherche 384, Faculte de Medecine, Clermont Ferrand, France
    Am J Hum Genet 72:1544-50. 2003
    ..In seven patients, we report for the first time mutations in three of the five EIF2B genes (EIF2B2, -4, and -5) that were recently shown to cause childhood ataxia with central nervous system ..
  4. ncbi Decreased guanine nucleotide exchange factor activity in eIF2B-mutated patients
    Anne Fogli
    INSERM UMR 384, Faculte de Medecine, 28 Place Henri Dunant, Clermont Ferrand, France
    Eur J Hum Genet 12:561-6. 2004
    Mutations in each of the five eucaryotic initiation factor 2B (eIF2B) subunits have been found in leukodystrophies of various severity: Cree leukoencephalopathy, childhood ataxia with central hypomyelination/leukodystrophy with vanishing ..
  5. ncbi Mutations in each of the five subunits of translation initiation factor eIF2B can cause leukoencephalopathy with vanishing white matter
    Marjo S van der Knaap
    Department of Child Neurology, Free University Medical Center, Amsterdam, The Netherlands
    Ann Neurol 51:264-70. 2002
    ..mutations in the genes encoding the epsilon- or the beta-subunit of the eukaryotic translation initiation factor eIF2B, a complex consisting of five subunits, cause the disease in most patients. Seven unsolved patients remained...
  6. pmc eIF2B-related disorders: antenatal onset and involvement of multiple organs
    Marjo S van der Knaap
    Department of Child Neurology, Free University Medical Center, Amsterdam, The Netherlands
    Am J Hum Genet 73:1199-207. 2003
    ..human disease related to mutations in any of the five genes encoding subunits of eukaryotic initiation factor eIF2B or any translation factor at all...
  7. pmc Possible mechanisms involved in the down-regulation of translation during transient global ischaemia in the rat brain
    C Martín De La Vega
    Departamento de Investigacion, Hospital Ramon y Cajal, Ctra Colmenar Km 9, 28034 Madrid, Spain
    Biochem J 357:819-26. 2001
    ..eIF)2, eIF4E and eIF4E-binding protein (4E-BP1) phosphorylation levels, eIF4F complex formation, as well as eIF2B and ribosomal protein S6 kinase (p70(S6K)) activities, were determined in different subcellular fractions from the ..
  8. ncbi Mechanism of inhibition of peptide chain initiation by amino acid deprivation in perfused rat liver. Regulation involving inhibition of eukaryotic initiation factor 2 alpha phosphatase activity
    S R Kimball
    Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey 17033
    J Biol Chem 266:1969-76. 1991
    ....
  9. ncbi Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B
    Yoshimitsu Kakuta
    Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812 8581, Japan
    Biochem Biophys Res Commun 319:725-32. 2004
    Eukaryotic translation initiation factor 2B (eIF2B) is the guanine-nucleotide exchange factor for eukaryotic initiation factor 2 (eIF2). eIF2B is a heteropentameric protein composed of alpha- subunits...
  10. pmc Inhibition of glycogen synthase kinase 3[beta] activity with lithium in vitro attenuates sepsis-induced changes in muscle protein turnover
    Stephen Bertsch
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA
    Shock 35:266-74. 2011
    ..the sepsis-induced increase in eukaryotic initiation factor (eIF) 2B[varepsilon] phosphorylation, decreased eIF2B activity, or the reduced phosphorylation of FOXO3, but instead was more closely associated with the continued ..
  11. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
    Eukaryotic translation initiation factor 2B (eIF2B) is the guanine nucleotide exchange factor (GEF) for eukaryotic translation initiation factor 2, which stimulates formation of the eIF2-GTP-Met-tRNA(i)(Met) ternary complex (TC) in a ..
  12. ncbi Deficiency of dietary EAA preferentially inhibits mRNA translation of ribosomal proteins in liver of meal-fed rats
    T G Anthony
    Department of Cellular and Molecular Physiology, Pennsylvania State College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 281:E430-9. 2001
    ..resulted in significant reductions in serum insulin, hepatic protein synthesis, eukaryotic initiation factor 2B (eIF2B) activity, and phosphorylation of eIF4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase (S6K1)...
  13. ncbi Expression and purification of the subunits of human translational initiation factor 2 (eIF2): phosphorylation of eIF2 alpha and beta
    Rajasekhar N V S Suragani
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India
    Protein Expr Purif 47:225-33. 2006
    ..alpha-subunit of eIF2 (eIF2alpha) and modulation of its interaction with a rate-limiting heteropentameric protein eIF2B. To understand the structural, functional, and regulatory roles of each of these subunits in the various ..
  14. doi Lithium chloride and staurosporine potentiate the accumulation of phosphorylated glycogen synthase kinase 3β/Tyr216, resulting in glycogen synthase kinase 3β activation in SH-SY5Y human neuroblastoma cell lines
    Anastasia Noël
    GReViC EA3808, PBS, bat IBMIG, Poitiers, France
    J Neurosci Res 89:755-63. 2011
    ..and regulates in turn through phosphorylation several proteins, including eukaryotic initiation factor 2B (eIF2B)...
  15. pmc Inhibition of glycogen synthase kinase-3beta is sufficient for airway smooth muscle hypertrophy
    Huan Deng
    Department of Pediatrics and Communicable Diseases, University of Michigan, 1150 W Medical Center Drive, Ann Arbor, MI 48109, USA
    J Biol Chem 283:10198-207. 2008
    ..attenuated LiCl- and SB216763-induced protein synthesis and expression of alpha-actin and SM22, indicating that eIF2B is required for GSK-3beta-mediated airway smooth muscle hypertrophy...
  16. pmc Acute and chronic ethanol consumption differentially impact pathways limiting hepatic protein synthesis
    Anne M Karinch
    Department of Cellular and Molecular Physiology, MC H166, Penn State Univ College of Medicine, 500 Univ Drive, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 295:E3-9. 2008
    ..steps: formation of the 43S preinitiation complex [controlled by eukaryotic initiation factors 2 (eIF2) and 2B (eIF2B)] and the binding of mRNA to the 40S ribosome (controlled by the eIF4F complex)...
  17. ncbi Identification of caspase 3-mediated cleavage and functional alteration of eukaryotic initiation factor 2alpha in apoptosis
    W E Marissen
    Department of Microbiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Biol Chem 275:9314-23. 2000
    ..showed that after caspase cleavage, exchange of GDP bound to eIF2 was very rapid and no longer dependent upon eIF2B. Furthermore, in vitro translation experiments indicated that cleavage of eIF2alpha results in functional ..
  18. ncbi Study of translational control of eukaryotic gene expression using yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Division of Intramural Research, NICHD, NIH, Bldg 18T, Rm 106, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 1038:60-74. 2004
    ..of translation initiation factor 2 (eIF2) and the attendant inhibition of its nucleotide exchange factor eIF2B-decreasing the binding to 40S ribosomes of methionyl initiator tRNA in the ternary complex (TC) with eIF2 and GTP...
  19. pmc Cloning and expression of cDNAs for the beta subunit of eukaryotic initiation factor-2B, the guanine nucleotide exchange factor for eukaryotic initiation factor-2
    B L Craddock
    Department of Biochemistry, School of Medical Sciences, University of Bristol U K
    Biochem J 309:1009-14. 1995
    ..Northern-blot analysis revealed a single major mRNA species for eIF-2B beta in each of the four rabbit tissues tested...
  20. ncbi Regulation of guanine nucleotide exchange through phosphorylation of eukaryotic initiation factor eIF2alpha. Role of the alpha- and delta-subunits of eiF2b
    S R Kimball
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 273:12841-5. 1998
    The guanine nucleotide exchange activity of eIF2B plays a key regulatory role in the translation initiation phase of protein synthesis. The activity is markedly inhibited when the substrate, i. e...
  21. pmc Fusel alcohols regulate translation initiation by inhibiting eIF2B to reduce ternary complex in a mechanism that may involve altering the integrity and dynamics of the eIF2B body
    Eleanor J Taylor
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Mol Biol Cell 21:2202-16. 2010
    ..This reaction is mediated by eIF2B, a heteropentameric factor with important links to human disease...
  22. pmc Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 17:1298-313. 1997
    b>eIF2B is a five-subunit guanine nucleotide exchange factor that is negatively regulated by phosphorylation of the alpha subunit of its substrate, eIF2, leading to inhibition of translation initiation...
  23. pmc Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2
    N T Price
    Department of Biochemistry, University of Bristol, U K
    Biochem J 318:631-6. 1996
    Peptide sequence data were obtained from rabbit protein synthesis initiation factor subunit eIF2B gamma...
  24. pmc An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation
    Chingakham Ranjit Singh
    Molecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, 66506, USA
    EMBO J 25:4537-46. 2006
    ..GDP binds the pentameric factor eIF2B for guanine nucleotide exchange...
  25. ncbi Thermal injury impairs cardiac protein synthesis and is associated with alterations in translation initiation
    Charles H Lang
    Department of Cellular and Molecular Physiology H166, Penn State College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Regul Integr Comp Physiol 286:R740-50. 2004
    ..Burn failed to alter eIF2B activity or the total amount or phosphorylation status of either eIF2 alpha or eIF2B epsilon in heart...
  26. pmc Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2
    K Asano
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    EMBO J 18:1673-88. 1999
    ..complex, and the resultant GDP on eIF2 is replaced with GTP by the complex nucleotide exchange factor, eIF2B. Bipartite motifs rich in aromatic and acidic residues are conserved at the C-termini of eIF5 and the catalytic (..
  27. ncbi Developmental changes in the feeding-induced stimulation of translation initiation in muscle of neonatal pigs
    T A Davis
    Department of Pediatrics, United States Department of Agriculture Agricultural Research Service, Children s Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Endocrinol Metab 279:E1226-34. 2000
    ..Each of these feeding-induced responses was blunted in muscle of 26-day-old pigs. eIF2B activity in muscle was unaffected by feeding status but decreased with development...
  28. pmc Conserved sequences in the beta subunit of archaeal and eukaryal translation initiation factor 2 (eIF2), absent from eIF5, mediate interaction with eIF2gamma
    G M Thompson
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Botucatu, 862, Sao Paulo, 04023 062, Brasil
    Biochem J 347:703-9. 2000
    ..aIF2beta differs from eIF2beta in not possessing an N-terminal extension implicated in binding RNA, eIF5 and eIF2B. The remaining sequences are highly conserved, and are shared with eIF5...
  29. ncbi Caerulein-induced acute pancreatitis inhibits protein synthesis through effects on eIF2B and eIF4F
    Maria Dolors Sans
    Department of Molecular and Integrative Physiology, The University of Michigan Medical School, Ann Arbor, MI 48109 0622, USA
    Am J Physiol Gastrointest Liver Physiol 285:G517-28. 2003
    ..We therefore evaluated in C57BL/6 mice the effects of caerulein-induced AP on pancreatic protein synthesis, eIF2B activity and other protein translation regulatory mechanisms...
  30. pmc eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Genes Dev 12:514-26. 1998
    b>eIF2B is a heteropentameric guanine-nucleotide exchange factor essential for protein synthesis initiation in eukaryotes...
  31. ncbi Impaired protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4E in muscle and eIF2B in liver
    C H Lang
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033 0850, USA
    Alcohol Clin Exp Res 24:322-31. 2000
    ..Acute alcohol intoxication in rats decreases protein synthesis in skeletal muscle and, to a lesser extent, in liver. The purpose of the present study was to examine potential mechanisms for the inhibitory effect of acute ethanol exposure...
  32. ncbi Endotoxin-induced decrease in muscle protein synthesis is associated with changes in eIF2B, eIF4E, and IGF-I
    C H Lang
    Departments of Cellular and Molecular Physiology, and Surgery, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Endocrinol Metab 278:E1133-43. 2000
    ..The content of eIF2alpha or the amount of eIF2alpha in the phosphorylated form did not change in response to LPS. eIF2B activity was decreased in muscle 4 h post-LPS but activity returned to control values by 24 h...
  33. pmc Reduced eukaryotic initiation factor 2Bepsilon-subunit expression suppresses the transformed phenotype of cells overexpressing the protein
    James W Gallagher
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Cancer Res 68:8752-60. 2008
    Eukaryotic initiation factor 2B (eIF2B), a five-subunit guanine nucleotide exchange factor, plays a key role in the regulation of mRNA translation...
  34. ncbi Eukaryotic initiation factors and protein synthesis after resistance exercise in rats
    P A Farrell
    Noll Physiological Research Center and Graduate Program in Physiology, Pennsylvania State University, University Park 16802, USA
    J Appl Physiol (1985) 88:1036-42. 2000
    ..J. Physiol. Endocrinol. Metab. 276: E721-E727, 1999) that increases in one factor, eIF2B, are associated with increases in rates of protein synthesis after resistance exercise in rats...
  35. pmc Archaeal translation initiation revisited: the initiation factor 2 and eukaryotic initiation factor 2B alpha-beta-delta subunit families
    N C Kyrpides
    Department of Microbiology, University of Illinois at Urbana Champaign, B103 Chemical and Life Sciences, MC 110, 407 S Goodwin, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 95:3726-30. 1998
    ..Overall, these data lend further support to the suggestion that the rudiments of translation initiation were present at the Universal Ancestor stage...
  36. ncbi Chronic alcohol feeding impairs hepatic translation initiation by modulating eIF2 and eIF4E
    C H Lang
    Department of Cellular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol 277:E805-14. 1999
    ..Alcohol feeding decreased hepatic eIF2B activity by 36%...
  37. ncbi Biochemical analysis of the eIF2beta gamma complex reveals a structural function for eIF2alpha in catalyzed nucleotide exchange
    J Nika
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75083, USA
    J Biol Chem 276:1051-6. 2001
    ..GDP complex that is recycled to the GTP form by the nucleotide exchange factor eIF2B. The alpha-subunit of eIF2 plays a critical role in regulating nucleotide exchange via phosphorylation at serine ..
  38. ncbi Phosphorylated seryl and threonyl, but not tyrosyl, residues are efficient specificity determinants for GSK-3beta and Shaggy
    D D Williams
    Department of Anatomy and Physiology, University of Dundee, UK
    FEBS Lett 448:86-90. 1999
    ..peptides derived from a putative glycogen synthase kinase-3 site in the Drosophila translation initiation factor eIF2B epsilon to investigate the efficacy of residues other than Ser(P) as priming residues for glycogen synthase kinase-..
  39. ncbi Regulation of protein synthesis by insulin
    C G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, Canada V6T 1Z3
    Biochem Soc Trans 34:213-6. 2006
    ..PKB acts to phosphorylate and inactivate glycogen synthase kinase 3, which in turn phosphorylates and inhibits eIF2B. Insulin elicits the dephosphorylation and activation of eIF2B...
  40. ncbi Direct binding of translation initiation factor eIF2gamma-G domain to its GTPase-activating and GDP-GTP exchange factors eIF5 and eIF2B epsilon
    Pankaj V Alone
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:12636-44. 2006
    ..The factor eIF5 stimulates hydrolysis of GTP by eIF2 upon AUG codon recognition, whereas the factor eIF2B promotes guanine nucleotide exchange on eIF2 to recycle the factor for additional rounds of translation initiation...
  41. ncbi Interplay between insulin and nutrients in the regulation of translation factors
    C G Proud
    Division of Molecular Physiology, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Biochem Soc Trans 29:541-7. 2001
    ..Insulin activates several of these proteins including the following: the guanine-nucleotide exchange factor eIF2B; the eIF4F complex, which (through eIF4E) interacts with the cap of the mRNA; p70 S6 kinase; and elongation factor ..
  42. doi Crystal structure of the C-terminal domain of the ɛ subunit of human translation initiation factor eIF2B
    Jia Wei
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Protein Cell 1:595-603. 2010
    Eukaryotic translation initiation factor eIF2B, the guanine nucleotide exchange factor (GEF) for eIF2, catalyzes conversion of eIF2·GDP to eIF2·GTP...
  43. ncbi Characterization of the initiation factor eIF2B and its regulation in Drosophila melanogaster
    D D Williams
    School of Life Sciences, Medical Sciences Institute Wellcome Trust Biocentre Complex, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 276:3733-42. 2001
    Eukaryotic initiation factor (eIF) 2B catalyzes a key regulatory step in the initiation of mRNA translation. eIF2B is well characterized in mammals and in yeast, although little is known about it in other eukaryotes...
  44. ncbi Initiation factor 2B activity is regulated by protein phosphatase 1, which is activated by the mitogen-activated protein kinase-dependent pathway in insulin-like growth factor 1-stimulated neuronal cells
    Celia Quevedo
    Servicio de Bioquímica Investigación, Hospital Ramon y Cajal, 28034 Madrid, Spain
    J Biol Chem 278:16579-86. 2003
    We have previously demonstrated that insulin-like growth factor 1 (IGF1) induces eukaryotic initiation factor 2B (eIF2B) activation in neuronal cells through the phosphatidylinositol 3 kinase/glycogen synthase kinase 3 pathway as well as ..
  45. ncbi eIF2 and the control of cell physiology
    Christopher G Proud
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dunde DD15EH, United Kingdom
    Semin Cell Dev Biol 16:3-12. 2005
    Eukaryotic initiation factor eIF2 and its 'exchange factor' eIF2B play a key role in the regulation of protein synthesis in eukaryotes from yeast to mammals. Phosphorylation of eIF2 inhibits eIF2B and thus translation initiation...
  46. ncbi Effect of CCK and intracellular calcium to regulate eIF2B and protein synthesis in rat pancreatic acinar cells
    Maria Dolors Sans
    Department of Physiology, University of Michigan, 1301 St Catherine St, 7737 Med Sci II, Ann Arbor, MI 48109, USA
    Am J Physiol Gastrointest Liver Physiol 282:G267-76. 2002
    ..Cholecystokinin (CCK) at 10-100 pM did not significantly affect eIF2B activity, which averaged 35...
  47. ncbi Insulin stimulates muscle protein synthesis in neonates during endotoxemia despite repression of translation initiation
    Renan A Orellana
    USDA ARS Children s Nutrition Research Center, Baylor College of Medicine, 1100 Bates St, Houston, TX 77030, USA
    Am J Physiol Endocrinol Metab 292:E629-36. 2007
    ..In muscle and liver, neither LPS nor insulin altered eIF2B activity. eEF2 phosphorylation decreased in response to insulin in both LPS and control animals...
  48. ncbi [Eukaryotic translation initiation factor 2B and leukoencephalopathy with vanishing white matter]
    Yan Xia Pan
    Department of Pediatric, Peking University First Hospital, Beijing 100034, China
    Beijing Da Xue Xue Bao 41:608-10. 2009
    ..with the gene defects in EIF2B1-5, encoding the five subunits of eukaryotic translation initiation factor (eIF2B alpha, beta, gamma, delta and epsilon ) respectively...
  49. ncbi Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs
    Jill A Bush
    United States Department of Agriculture Agricultural Research Service Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Endocrinology 144:1273-83. 2003
    ..In muscle, but not liver, GH increased eIF2B activity and 4E-BP1 phosphorylation in both the fasted and fed state and increased the association of eIF4E with ..
  50. ncbi Regulation of translation initiation by insulin and amino acids in skeletal muscle of neonatal pigs
    Pamela M J O'Connor
    United States Department of Agriculture Agricultural Research Service, Children s Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Endocrinol Metab 285:E40-53. 2003
    ..to eIF4E, increased eIF4E binding to eIF4G, and increased fractional protein synthesis rates but did not affect eIF2B activity...
  51. ncbi Type 1 phosphatase inhibitors reduce the restoration of guanine nucleotide exchange activity of eukaryotic initiation factor 2B inhibited reticulocyte lysates rescued by hemin
    S V Babu
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, India
    Arch Biochem Biophys 327:201-8. 1996
    ..These findings suggest that a type 1 phosphatase is involved in the recovery of eIF-2B activity and protein synthesis upon delayed addition of hemin to heme-deficient lysates...
  52. pmc DYRK2 negatively regulates cardiomyocyte growth by mediating repressor function of GSK-3β on eIF2Bε
    Celine S Weiss
    Internal Medicine III, University Hospital Heidelberg and DZHK German Center for Cardiovascular Research, Heidelberg, Germany
    PLoS ONE 8:e70848. 2013
    ..A central regulator of translation initiation is Eukaryotic initiation factor 2B (eIF2B)...
  53. ncbi Regulation of muscle protein synthesis in neonatal pigs during prolonged endotoxemia
    Renan A Orellana
    USDA ARS Children s Nutrition Research Center, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USA
    Pediatr Res 55:442-9. 2004
    ..In muscle and liver, LPS did not alter eIF2B activity, an event that regulates initiator met-tRNA(i) binding to the 40S ribosomal complex...
  54. doi Severity of vanishing white matter disease does not correlate with deficits in eIF2B activity or the integrity of eIF2B complexes
    Rui Liu
    Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, People s Republic of China
    Hum Mutat 32:1036-45. 2011
    Autosomal recessive mutations in eukaryotic initiation factor 2B (eIF2B) cause leukoencephalopathy vanishing white matter with a wide clinical spectrum...
  55. ncbi Localization of the translational guanine nucleotide exchange factor eIF2B: a common theme for GEFs?
    Susan G Campbell
    Faculty of Life Sciences, The University of Manchester, Manchester, UK
    Cell Cycle 5:678-80. 2006
    The eukaryotic initiation factor 2B (eIF2B) serves an essential recycling function in protein synthesis...
  56. doi Leukodystrophies with astrocytic dysfunction
    Diana Rodriguez
    Université Pierre et Marie Curie Paris 6 and Hôpital Armand Trousseau, Paris, France Electronic address
    Handb Clin Neurol 113:1619-28. 2013
    ..CACH/VWM is due to mutations in one of the five subunits of EIF2B which compromise the astrocytic lineage...
  57. ncbi Identification of ten novel mutations in patients with eIF2B-related disorders
    Andreas Ohlenbusch
    Department of Pediatrics and Pediatric Neurology, Georg August University, Gottingen, Germany
    Hum Mutat 25:411. 2005
    ..phenotypic variants like ovarioleukodystrophy were investigated for mutations in the genes EIF2B1, EIF2B2, EIF2B3, EIF2B4, and EIF2B5 encoding eIF2B. We identified one novel nonsense mutation (EIF2B4, c.625C>T, p...
  58. doi Differentiation of rat iPS cells and ES cells into granulosa cell-like cells in vitro
    Juan Zhang
    Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha 410083, China
    Acta Biochim Biophys Sin (Shanghai) 45:289-95. 2013
    ..to analyze the expression level of marker genes in POF, including BMP15, FMR1, FSHR, INHA, AMH, NOBOX, FOXO3, EIF2B, FIGLA, and GDF9...
  59. ncbi eIF2B, a mediator of general and gene-specific translational control
    G D Pavitt
    Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Biochem Soc Trans 33:1487-92. 2005
    b>eIF2B (eukaryotic initiation factor 2B) is a multisubunit protein that is required for protein synthesis initiation and its regulation in all eukaryotic cells...
  60. ncbi Trivalent chromium alters gene expression in the mummichog (Fundulus heteroclitus)
    Nikki L Maples
    Department of Biological Sciences, University of Texas at El Paso, El Paso, Texas 79968, USA
    Environ Toxicol Chem 23:626-31. 2004
    ..acid-binding protein (FABP), cytochrome P4502N2 (CYP2N2), and a precursor to the translation initiation factor eIF2B. Verification of the differentially expressed genes by real-time polymerase chain reaction (PCR) revealed that the ..
  61. pmc Dynamic cycling of eIF2 through a large eIF2B-containing cytoplasmic body: implications for translation control
    Susan G Campbell
    Faculty of Life Science, The University of Manchester, Manchester, M13 9PT, England, UK
    J Cell Biol 170:925-34. 2005
    The eukaryotic translation initiation factor 2B (eIF2B) provides a fundamental controlled point in the pathway of protein synthesis...
  62. pmc Guanine nucleotide exchange factor for eukaryotic translation initiation factor 2 in Saccharomyces cerevisiae: interactions between the essential subunits GCD2, GCD6, and GCD7 and the regulatory subunit GCN3
    J L Bushman
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:4618-31. 1993
    ....
  63. ncbi Inactivation of eIF2B and phosphorylation of PHAS-I in heat-shocked rat hepatoma cells
    G C Scheper
    Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 272:26850-6. 1997
    ..In this respect, the activity of eIF2B was measured during and after heat shock...
  64. ncbi Regulation of mammalian translation factors by nutrients
    Christopher G Proud
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, UK
    Eur J Biochem 269:5338-49. 2002
    ..important regulatory step in translation initiation, the activity of the guanine nucleotide-exchange factor eIF2B. eIF2B controls the recruitment of the initiator methionyl-tRNA to the ribosome and is activated by insulin...
  65. pmc ced-4 and proto-oncogene tfg-1 antagonistically regulate cell size and apoptosis in C. elegans
    Ling Chen
    Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA
    Curr Biol 18:1025-33. 2008
    ..Apaf1/CED-4 is essential for apoptosis but has not been shown to function in cell-growth control...
  66. pmc Minimum requirements for the function of eukaryotic translation initiation factor 2
    F L Erickson
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75083 0688, USA
    Genetics 158:123-32. 2001
    ..b>eIF2B, the nucleotide exchange factor for eIF2, is a heteropentamer that, in yeast, is encoded by four essential genes ..
  67. ncbi Differing substrate specificities of members of the DYRK family of arginine-directed protein kinases
    Linda E Campbell
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    FEBS Lett 510:31-6. 2002
    ..We recently showed that DYRK2 phosphorylates the translation initiation factor eIF2B at Ser539 in its epsilon-subunit and thereby "primes" its phosphorylation by glycogen synthase kinase-3...
  68. ncbi Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue
    Thomas Boesen
    Department of Molecular Biology, Aarhus University, Denmark
    J Biol Chem 279:10584-92. 2004
    ....
  69. ncbi Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae
    J Nika
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080, USA
    J Biol Chem 275:26011-7. 2000
    Eukaryotic translation initiation factor 2B (eIF2B) is the heteropentameric guanine nucleotide exchange factor for translation initiation factor 2 (eIF2)...
  70. ncbi Reducing agents mitigate protein synthesis inhibition mediated by vanadate and vanadyl compounds in reticulocyte lysates
    T Krishnamoorthy
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, India
    Arch Biochem Biophys 349:122-8. 1998
    ..of the alpha (alpha) subunit of initiation factor 2 (eIF2) or decrease the guanine nucleotide exchange activity of eIF2B, which is required to exchange GDP for GTP in eIF2.GDP binary complex...
  71. pmc Discovery of chemical modulators of a conserved translational control pathway by parallel screening in yeast
    Nuzhat Motlekar
    Penn Center for Molecular Discovery, Institute for Medicine and Engineering, and Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Assay Drug Dev Technol 7:479-94. 2009
    ..In addition, inherited mutations in human eIF2B subunits cause a fatal brain disorder commonly called childhood ataxia with central nervous system hypomyelination ..
  72. ncbi [From gene to disease; a defect in the regulation of protein production leading to vanishing white matter]
    J C Pronk
    Afd Klinische en Humane Genetica, Vrije Universiteit Medisch Centrum, Postbus 7057, 1007 MB Amsterdam
    Ned Tijdschr Geneeskd 146:1933-6. 2002
    ..We recently identified the five genes associated with VWM: EIF2B1-5. They encode the five subunits of eIF2B, which is a eukaryotic translation initiation factor expressed in all human tissues and highly conserved during ..
  73. ncbi Glycogen synthase kinase-3 is the predominant insulin-regulated eukaryotic initiation factor 2B kinase in skeletal muscle
    L S Jefferson
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey 17033, USA
    Int J Biochem Cell Biol 31:191-200. 1999
    Eukaryotic initiation factor eIF2B is a guanine nucleotide exchange protein involved in regulation of translation initiation. Phosphorylation of the epsilon-subunit is thought to be important in insulin-mediated changes in eIF2B activity...
  74. pmc eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation
    Martin D Jennings
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Nature 465:378-81. 2010
    ..of the eIF2(alphaP) regulatory complex that inhibits the activity of the guanine-nucleotide exchange factor (GEF) eIF2B. Together our studies define a new step in the translation initiation pathway, one that is critical for normal ..
  75. doi Crystal structure of the alpha subunit of human translation initiation factor 2B
    Takuya B Hiyama
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Mol Biol 392:937-51. 2009
    Eukaryotic translation initiation factor 2B (eIF2B) is the heteropentameric guanine-nucleotide exchange factor specific for eukaryotic initiation factor 2 (eIF2)...
  76. doi Intersubunit and interprotein interactions of alpha- and beta-subunits of human eIF2: Effect of phosphorylation
    Kamindla Rajesh
    Department of Biochemistry, University of Hyderabad, Gachibowli, Hyderabad 500 046, Andhra Pradesh, India
    Biochem Biophys Res Commun 374:336-40. 2008
    ..Recombinant phosphorylated human eIF2alpha that cannot interact with purified eIF2B, the GDP/GTP exchange factor of eIF2, however interacts efficiently with eIF2B along with the beta-subunit of eIF2 ..
  77. doi Protein synthesis and its control in neuronal cells with a focus on vanishing white matter disease
    Graham D Pavitt
    Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, UK
    Biochem Soc Trans 37:1298-310. 2009
    ..VWM/CACH is caused by mutations in the translation initiation factor, eIF2B, a component of the basal translational machinery in all cells.
  78. doi Identification of novel EIF2B mutations in Chinese patients with vanishing white matter disease
    Ye Wu
    Department of Pediatrics, Peking University First Hospital, Beijing, China
    J Hum Genet 54:74-7. 2009
    ..in EIF2B1-5 (identified in 2001-2002) encoding the five subunits of eukaryotic translation initiation factor (eIF2B alpha, beta, gamma, delta and epsilon), respectively...
  79. ncbi Vanishing white matter disease
    Marjo S van der Knaap
    Department of Pediatrics and Child Neurology, VU University Medical Center, Amsterdam, Netherlands
    Lancet Neurol 5:413-23. 2006
    ..of this striking disease resides in either one of the five subunits of eukaryotic translation initiation factor eIF2B. eIF2B is essential in all cells of the body for protein synthesis and its regulation under different stress ..
  80. pmc Translation initiation factors are not required for Dicistroviridae IRES function in vivo
    Nilsa Deniz
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    RNA 15:932-46. 2009
    ..CrPV IGR IRES activity does not require the eukaryotic initiation factors eIF4G1 or eIF5B, and it is enhanced when eIF2B, the eIF3b subunit of eIF3, or eIF4E are impaired...
  81. pmc Archaeal aIF2B interacts with eukaryotic translation initiation factors eIF2alpha and eIF2Balpha: Implications for aIF2B function and eIF2B regulation
    Kamal Dev
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Mol Biol 392:701-22. 2009
    ..the alpha-subunit of eIF2 (eukaryotic initiation factor 2), which inhibits its guanine nucleotide exchange factor, eIF2B. The N-terminal S1 domain of phosphorylated eIF2alpha interacts with a subcomplex of eIF2B formed by the three ..
  82. doi Roles of GSK3 in metabolic shift toward abnormal anabolism in cell senescence
    You Mie Kim
    Department of Biochemistry and Molecular Biology, The Graduate School, Ajou University, Suwon, Korea
    Ann N Y Acad Sci 1201:65-71. 2010
    ..Anabolism was accomplished through glycogen synthase, eIF2B, and SREBP1...
  83. pmc eIF2B and oligodendrocyte survival: where nature and nurture meet in bipolar disorder and schizophrenia?
    Christopher J Carter
    Schizophr Bull 33:1343-53. 2007
    ..protein synthesis via the eventual inhibition, by phosphorylated eIF2-alpha, of the translation initiation factor eIF2B. Growth factors increase protein synthesis via eIF2B activation and counterbalance this system...
  84. ncbi Arg113His mutation of vanishing white matter is not present in multiple sclerosis
    M Lucas
    Molecular Biology Service, Virgen Macarena University Hospital, Seville, Spain
    Mult Scler 13:424-7. 2007
    ..Our report does not exclude the presence in MS chromosomes of other mutations in the eIF2B gene family.
  85. doi Exon deletion in the non-catalytic domain of eIF2Bepsilon due to a splice site mutation leads to infantile forms of CACH/VWM with severe decrease of eIF2B GEF activity
    L Horzinski
    INSERM UMR384, Faculte de Medecine, 28 Place Henri Dunant, F 63003 Clermont Ferrand, France
    Ann Hum Genet 72:410-5. 2008
    ..mutations occurring in the five genes (EIF2B1-5) that encode the translation eukaryotic initiation factor eIF2B. We found that a patient with infantile CACH/VWM carries a mutation in the acceptor splice site of EIF2B5 exon 6...
  86. doi Genetic and clinical heterogeneity in eIF2B-related disorder
    Jelena Maletkovic
    Children s National Medical Center, Children s Research Institute, Center for Genetic Medicine, Washington, DC 20010, USA
    J Child Neurol 23:205-15. 2008
    Eukaryotic initiation factor 2B (eIF2B)-related disorders are heritable white matter disorders with a variable clinical phenotype (including vanishing white matter disease and ovarioleukodystrophy) and an equally heterogeneous genotype...
  87. doi Conversion of a normal MRI into an MRI showing a cystic leukoencephalopathy is not a known feature of vanishing white matter
    M S van der Knaap
    Neuropediatrics 38:264. 2007
  88. pmc Simvastatin represses protein synthesis in the muscle-derived C₂C₁₂ cell line with a concomitant reduction in eukaryotic initiation factor 2B expression
    Alexander P Tuckow
    Dept of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 300:E564-70. 2011
    ..synthesis occurred with a concomitant decrease in expression and activity of eukaryotic initiation factor 2B (eIF2B), a regulated and rate-controlling guanine nucleotide exchange factor known to affect global rates of protein ..
  89. pmc Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis
    Neil Kubica
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, United States
    Int J Biochem Cell Biol 40:2522-33. 2008
    ..and a stimulation of new eIF2Bepsilon protein synthesis, but had no effect on mRNAs encoding the other four eIF2B subunits...
  90. doi Functional analysis of recently identified mutations in eukaryotic translation initiation factor 2Bɛ (eIF2Bɛ) identified in Chinese patients with vanishing white matter disease
    Xuerong Leng
    Pediatric Department, Peking University First Hospital, Beijing, China
    J Hum Genet 56:300-5. 2011
    ..Gene defects in each of the five subunits of eukaryotic translation initiation factor 2B (eIF2B α-ɛ) are responsible for the disease, although the mechanism of the pathogenesis is not well understood...
  91. doi Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics
    Keiji Kito
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan
    Proteomics 8:2366-70. 2008
    ..We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.
  92. doi Clues to the mechanism of action of eIF2B, the guanine-nucleotide-exchange factor for translation initiation
    Sarah S Mohammad-Qureshi
    Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester, UK
    Biochem Soc Trans 36:658-64. 2008
    ..eukaryotic translation initiation between eIF (eukaryotic initiation factor) 2, a G-protein, its five-subunit GEF, eIF2B, and its GAP, eIF5...
  93. ncbi Acute neurological deterioration in ovarioleukodystrophy related to EIF2B mutations: pregnancy with oocyte donation is a potentially precipitating factor
    L Peter
    CHU de Lyon, Service d Epileptologie, Hopital Neurologique, Lyon Cedex 3, France
    Eur J Neurol 15:94-7. 2008
    Mutations in the eukaryotic translation initiation factor 2B (eIF2B) represent a heterogenous group of autosomal recessive leucodystrophy characterized by a diffuse CSF-like aspect of the white matter at MRI designed as vanishing white ..
  94. pmc Change in nutritional status modulates the abundance of critical pre-initiation intermediate complexes during translation initiation in vivo
    Chingakham Ranjit Singh
    Molecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    J Mol Biol 370:315-30. 2007
    ..TC) interacts with eIF3-eIF1-eIF5 complex to form the multifactor complex (MFC), while eIF2GDP associates with eIF2B for guanine nucleotide exchange. Gcn2p phosphorylates eIF2 to inhibit eIF2B...
  95. ncbi A role for eukaryotic translation initiation factor 2B (eIF2B) in taste memory consolidation and in thermal control establishment during the critical period for sensory development
    Sharon Tirosh
    Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
    Dev Neurobiol 67:728-39. 2007
    ..It was established that eIF2Bepsilon-mRNA was induced in the preopotic anterior hypothalamus during heat conditioning. Taken together, these results correlate eIF2Bepsilon with sensory development...
  96. ncbi Eukaryotic type translation initiation factor 2: structure-functional aspects
    E A Stolboushkina
    Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
    Biochemistry (Mosc) 76:283-94. 2011
    ..There are also new data on initiation factors eIF5 and eIF2B that directly interact with eIF2 and control its participation in nucleotide exchange.
  97. ncbi Reduced eIF2alpha phosphorylation and increased proapoptotic proteins in aging
    Syed G Hussain
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, AP, India
    Biochem Biophys Res Commun 355:365-70. 2007
    ..Concomitant with increased eIF2alpha phosphorylation, young tissues displayed a higher level of eIF2B to tolerate the toxic effect of eIF2alpha phosphorylation on translation, ATF4, a b-zip transcriptional factor ..
  98. pmc eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast
    N T Price
    Department of Biochemistry, University of Bristol, U K
    Biochem J 318:637-43. 1996
    The guanine nucleotide-exchange factor eIF2B mediates the exchange of GDP bound to translation initiation factor eIF2 for GTP...
  99. pmc Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae
    J L Bushman
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:1920-32. 1993
    ..Together, our results provide evidence that GCD6 and GCD7 are subunits of eIF-2B in S. cerevisiae and further implicate this GDP-GTP exchange factor in gene-specific translational control...
  100. ncbi Regulation of eukaryotic initiation factor-2B activity in muscle of diabetic rats
    A M Karinch
    Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey 17033
    Am J Physiol 264:E101-8. 1993
    ..Modulation of casein kinase II activity may be a factor in the regulation of protein synthesis in muscle during acute diabetes. The activity of eIF-2B in heart might be maintained by the increased NADPH/NADP+...
  101. ncbi AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling
    Douglas R Bolster
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 277:23977-80. 2002
    ..A reduction in eIF4E associated with eIF4G to 10% of the control value was also noted. In contrast, eIF2B activity remained unchanged in response to AICAR treatment and therefore would not appear to contribute to the ..

Research Grants75

  1. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    Jeffrey Bonanno; Fiscal Year: 2002
    ..of an important subset of the remaining translation initiation factors [eIF4G, eIF4A, eIF2, IF2, eIF3, eIF1A, eIF2B, eIF5, the poly (A)-binding protein (PABP), and a PABP-interacting protein]...
  2. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..of an important subset of the remaining translation initiation factors [eIF4G, eIF4A, eIF2, IF2, eIF3, eIF1A, eIF2B, eIF5, the poly (A)-binding protein (PABP), and a PABP-interacting protein]...
  3. Amino Acids and Muscle Protein Synthesis
    Robert Wolfe; Fiscal Year: 2006
    ..to result in increased muscle protein synthesis unless there is sufficient charging of tRNA and activity of eIF2B. We propose that in the circumstance of an adequate basal amount of amino acids the transient stimulation of ..
  4. Cell biological analysis of CACH/VWM leukodystrophy
    Christoph Proschel; Fiscal Year: 2005
    ..has not been the case for the recently identified mutations in the subunits of translation initiation factor 2B (eIF2B) in patients diagnosed with autosomal recessive Childhood Ataxia with Diffuse CNS Hypomyelination (CACH)/Vanishing ..
  5. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    THOMAS VARY; Fiscal Year: 2002
    ..at two steps: formation of the 43S pre-initiation complex (controlled by eukaryotic initiation factor 2 (eIF2) and eIF2B); and the binding of mRNA to the 40S ribosome (controlled by elF4E)...
  6. REGULATION OF LIVER GROWTH AND FUNCTION
    Leonard Jefferson; Fiscal Year: 2004
    ..insulin, alter the synthesis of both total and specific hepatic routines via coordinated modulation of the eIF2/eIF2B, eIF4F and P70 S 6 K regulatory steps in translation initiation and the RNA and polymerase 1 step in ribosome bio ..
  7. REGULATION OF LIVER GROWTH AND FUNCTION
    Leonard Jefferson; Fiscal Year: 1999
    ..They should also provide new insights into the biochemical and molecular mechanisms involved in the initiation of protein synthesis. ..
  8. REGULATION OF SKELETAL MUSCLE METABOLISM
    Leonard Jefferson; Fiscal Year: 1999
    ..They should also provide new insights into the biochemical and molecular mechanisms involved in translation initiation. ..
  9. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  10. Cytokine Regulation of Muscle Protein Synthesis During Infection
    Charles Lang; Fiscal Year: 2009
    ..Our research will determine the mechanism for this muscle atrophy which will ultimately lead to development of novel therapeutic treatment and improve patient outcome. ..
  11. MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATION
    Charles H Lang; Fiscal Year: 2010
    ..Overall, the research design will establish the mechanism by which myocardial protein synthesis is reduced in response to alcohol abuse. ..
  12. Myocardial Protein Synthesis After Thermal Injury
    Charles Lang; Fiscal Year: 2005
    ..these changes are mediated by TNFa; (2) to determine the mechanism by which thermal injury decreases activity of eIF2B in heart, by assessing the phosphorylation of elF2Be, content of p67, and the importance of alterations in kinase ..
  13. Regulation of Nutrient Sensing and Muscle Wasting by Alcohol
    Charles Lang; Fiscal Year: 2009
    ..The proposed studies have a translational underpinning in that the data may lead to development of novel clinical interventions designed to reverse alcoholic myopathy during rehabilitation. ..
  14. Regulation of Nutrient Sensing and Muscle Wasting by Alcohol
    Charles H Lang; Fiscal Year: 2010
    ..The proposed studies have a translational underpinning in that the data may lead to development of novel clinical interventions designed to reverse alcoholic myopathy during rehabilitation. ..
  15. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  16. CYTOKINE REGULATION OF IGF SYSTEM DURING INFECTION
    Charles Lang; Fiscal Year: 2007
    ..abstract_text> ..
  17. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles Lang; Fiscal Year: 2007
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  18. AntiretroviraI induced Defects in Muscle Protein Synthes
    Charles Lang; Fiscal Year: 2009
    ..Such data are needed to both realize the full potential and avoid possible pitfalls of this drug class in long-term treatment of HIV infection. ..
  19. Regulation of Nutrient Sensing and Muscle Wasting by Alcohol
    Charles Lang; Fiscal Year: 2007
    ..The proposed studies have a translational underpinning in that the data may lead to development of novel clinical interventions designed to reverse alcoholic myopathy during rehabilitation. ..
  20. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles Lang; Fiscal Year: 2009
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  21. Cytokine Regulation of Muscle Protein Synthesis During Infection
    Charles H Lang; Fiscal Year: 2010
    ..Our research will determine the mechanism for this muscle atrophy which will ultimately lead to development of novel therapeutic treatment and improve patient outcome. ..
  22. REGULATION OF IGF SYSTEM AND MUSCLE WASTING BY ALCOHOL
    Charles Lang; Fiscal Year: 2001
    ....
  23. CYTOKINE REGULATION OF IGF SYSTEM DURING INFECTION
    Charles Lang; Fiscal Year: 1999
    ..These experiments will be conducted on cultured L6 myotubes, and protein synthesis, breakdown and amino acid transport will be quantitated. ..
  24. CYTOKINE REGULATION OF IGF SYSTEM DURING INFECTION
    Charles Lang; Fiscal Year: 2003
    ..abstract_text> ..
  25. REGULATION OF SKELETAL MUSCLE METABOLISM
    Leonard Jefferson; Fiscal Year: 1993
    ..They should also provide new insights into the biochemical and molecular mechanisms involved in the initiation of protein synthesis...
  26. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    Scot R Kimball; Fiscal Year: 2010
    ....
  27. INSULIN & PULSATILITY
    PETER FARRELL; Fiscal Year: 2000
    ..We will document whether part of the cause for increased insulin resistance in older persons is partially due to an altered insulin pulse profile. ..
  28. INSULIN & PULSATILITY
    PETER FARRELL; Fiscal Year: 2002
    ..We will document whether part of the cause for increased insulin resistance in older persons is partially due to an altered insulin pulse profile. ..