EIF2B2

Summary

Gene Symbol: EIF2B2
Description: eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Alias: EIF-2Bbeta, EIF2B, S20I15, S20III15, eIF-2B GDP-GTP exchange factor subunit beta, translation initiation factor eIF-2B subunit beta
Species: human

Top Publications

  1. doi Lithium chloride and staurosporine potentiate the accumulation of phosphorylated glycogen synthase kinase 3β/Tyr216, resulting in glycogen synthase kinase 3β activation in SH-SY5Y human neuroblastoma cell lines
    Anastasia Noël
    GReViC EA3808, PBS, bat IBMIG, Poitiers, France
    J Neurosci Res 89:755-63. 2011
  2. pmc Ovarian failure related to eukaryotic initiation factor 2B mutations
    Anne Fogli
    INSERM Unité Mixte de Recherche 384, Faculte de Medecine, Clermont Ferrand, France
    Am J Hum Genet 72:1544-50. 2003
  3. pmc Possible mechanisms involved in the down-regulation of translation during transient global ischaemia in the rat brain
    C Martín De La Vega
    Departamento de Investigacion, Hospital Ramon y Cajal, Ctra Colmenar Km 9, 28034 Madrid, Spain
    Biochem J 357:819-26. 2001
  4. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
  5. ncbi Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B
    Yoshimitsu Kakuta
    Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812 8581, Japan
    Biochem Biophys Res Commun 319:725-32. 2004
  6. ncbi Expression and purification of the subunits of human translational initiation factor 2 (eIF2): phosphorylation of eIF2 alpha and beta
    Rajasekhar N V S Suragani
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India
    Protein Expr Purif 47:225-33. 2006
  7. ncbi Deficiency of dietary EAA preferentially inhibits mRNA translation of ribosomal proteins in liver of meal-fed rats
    T G Anthony
    Department of Cellular and Molecular Physiology, Pennsylvania State College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 281:E430-9. 2001
  8. pmc Acute and chronic ethanol consumption differentially impact pathways limiting hepatic protein synthesis
    Anne M Karinch
    Department of Cellular and Molecular Physiology, MC H166, Penn State Univ College of Medicine, 500 Univ Drive, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 295:E3-9. 2008
  9. pmc Minimum requirements for the function of eukaryotic translation initiation factor 2
    F L Erickson
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75083 0688, USA
    Genetics 158:123-32. 2001
  10. pmc Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 17:1298-313. 1997

Research Grants

Scientific Experts

Detail Information

Publications142 found, 100 shown here

  1. doi Lithium chloride and staurosporine potentiate the accumulation of phosphorylated glycogen synthase kinase 3β/Tyr216, resulting in glycogen synthase kinase 3β activation in SH-SY5Y human neuroblastoma cell lines
    Anastasia Noël
    GReViC EA3808, PBS, bat IBMIG, Poitiers, France
    J Neurosci Res 89:755-63. 2011
    ..and regulates in turn through phosphorylation several proteins, including eukaryotic initiation factor 2B (eIF2B)...
  2. pmc Ovarian failure related to eukaryotic initiation factor 2B mutations
    Anne Fogli
    INSERM Unité Mixte de Recherche 384, Faculte de Medecine, Clermont Ferrand, France
    Am J Hum Genet 72:1544-50. 2003
    ..In seven patients, we report for the first time mutations in three of the five EIF2B genes (EIF2B2, -4, and -5) that were recently shown to cause childhood ataxia with central nervous system hypomyelination/..
  3. pmc Possible mechanisms involved in the down-regulation of translation during transient global ischaemia in the rat brain
    C Martín De La Vega
    Departamento de Investigacion, Hospital Ramon y Cajal, Ctra Colmenar Km 9, 28034 Madrid, Spain
    Biochem J 357:819-26. 2001
    ..eIF)2, eIF4E and eIF4E-binding protein (4E-BP1) phosphorylation levels, eIF4F complex formation, as well as eIF2B and ribosomal protein S6 kinase (p70(S6K)) activities, were determined in different subcellular fractions from the ..
  4. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
    Eukaryotic translation initiation factor 2B (eIF2B) is the guanine nucleotide exchange factor (GEF) for eukaryotic translation initiation factor 2, which stimulates formation of the eIF2-GTP-Met-tRNA(i)(Met) ternary complex (TC) in a ..
  5. ncbi Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B
    Yoshimitsu Kakuta
    Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812 8581, Japan
    Biochem Biophys Res Commun 319:725-32. 2004
    Eukaryotic translation initiation factor 2B (eIF2B) is the guanine-nucleotide exchange factor for eukaryotic initiation factor 2 (eIF2). eIF2B is a heteropentameric protein composed of alpha- subunits...
  6. ncbi Expression and purification of the subunits of human translational initiation factor 2 (eIF2): phosphorylation of eIF2 alpha and beta
    Rajasekhar N V S Suragani
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India
    Protein Expr Purif 47:225-33. 2006
    ..alpha-subunit of eIF2 (eIF2alpha) and modulation of its interaction with a rate-limiting heteropentameric protein eIF2B. To understand the structural, functional, and regulatory roles of each of these subunits in the various ..
  7. ncbi Deficiency of dietary EAA preferentially inhibits mRNA translation of ribosomal proteins in liver of meal-fed rats
    T G Anthony
    Department of Cellular and Molecular Physiology, Pennsylvania State College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 281:E430-9. 2001
    ..resulted in significant reductions in serum insulin, hepatic protein synthesis, eukaryotic initiation factor 2B (eIF2B) activity, and phosphorylation of eIF4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase (S6K1)...
  8. pmc Acute and chronic ethanol consumption differentially impact pathways limiting hepatic protein synthesis
    Anne M Karinch
    Department of Cellular and Molecular Physiology, MC H166, Penn State Univ College of Medicine, 500 Univ Drive, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 295:E3-9. 2008
    ..steps: formation of the 43S preinitiation complex [controlled by eukaryotic initiation factors 2 (eIF2) and 2B (eIF2B)] and the binding of mRNA to the 40S ribosome (controlled by the eIF4F complex)...
  9. pmc Minimum requirements for the function of eukaryotic translation initiation factor 2
    F L Erickson
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75083 0688, USA
    Genetics 158:123-32. 2001
    ..b>eIF2B, the nucleotide exchange factor for eIF2, is a heteropentamer that, in yeast, is encoded by four essential genes ..
  10. pmc Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 17:1298-313. 1997
    b>eIF2B is a five-subunit guanine nucleotide exchange factor that is negatively regulated by phosphorylation of the alpha subunit of its substrate, eIF2, leading to inhibition of translation initiation...
  11. ncbi Inactivation of eIF2B and phosphorylation of PHAS-I in heat-shocked rat hepatoma cells
    G C Scheper
    Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 272:26850-6. 1997
    ..In this respect, the activity of eIF2B was measured during and after heat shock...
  12. ncbi Study of translational control of eukaryotic gene expression using yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Division of Intramural Research, NICHD, NIH, Bldg 18T, Rm 106, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 1038:60-74. 2004
    ..of translation initiation factor 2 (eIF2) and the attendant inhibition of its nucleotide exchange factor eIF2B-decreasing the binding to 40S ribosomes of methionyl initiator tRNA in the ternary complex (TC) with eIF2 and GTP...
  13. ncbi Identification of caspase 3-mediated cleavage and functional alteration of eukaryotic initiation factor 2alpha in apoptosis
    W E Marissen
    Department of Microbiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Biol Chem 275:9314-23. 2000
    ..showed that after caspase cleavage, exchange of GDP bound to eIF2 was very rapid and no longer dependent upon eIF2B. Furthermore, in vitro translation experiments indicated that cleavage of eIF2alpha results in functional ..
  14. pmc Fusel alcohols regulate translation initiation by inhibiting eIF2B to reduce ternary complex in a mechanism that may involve altering the integrity and dynamics of the eIF2B body
    Eleanor J Taylor
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Mol Biol Cell 21:2202-16. 2010
    ..This reaction is mediated by eIF2B, a heteropentameric factor with important links to human disease...
  15. ncbi Regulation of guanine nucleotide exchange through phosphorylation of eukaryotic initiation factor eIF2alpha. Role of the alpha- and delta-subunits of eiF2b
    S R Kimball
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 273:12841-5. 1998
    The guanine nucleotide exchange activity of eIF2B plays a key regulatory role in the translation initiation phase of protein synthesis. The activity is markedly inhibited when the substrate, i. e...
  16. pmc Inhibition of glycogen synthase kinase-3beta is sufficient for airway smooth muscle hypertrophy
    Huan Deng
    Department of Pediatrics and Communicable Diseases, University of Michigan, 1150 W Medical Center Drive, Ann Arbor, MI 48109, USA
    J Biol Chem 283:10198-207. 2008
    ..attenuated LiCl- and SB216763-induced protein synthesis and expression of alpha-actin and SM22, indicating that eIF2B is required for GSK-3beta-mediated airway smooth muscle hypertrophy...
  17. pmc An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation
    Chingakham Ranjit Singh
    Molecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, 66506, USA
    EMBO J 25:4537-46. 2006
    ..GDP binds the pentameric factor eIF2B for guanine nucleotide exchange...
  18. pmc Discovery of chemical modulators of a conserved translational control pathway by parallel screening in yeast
    Nuzhat Motlekar
    Penn Center for Molecular Discovery, Institute for Medicine and Engineering, and Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Assay Drug Dev Technol 7:479-94. 2009
    ..In addition, inherited mutations in human eIF2B subunits cause a fatal brain disorder commonly called childhood ataxia with central nervous system hypomyelination ..
  19. pmc Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2
    K Asano
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    EMBO J 18:1673-88. 1999
    ..complex, and the resultant GDP on eIF2 is replaced with GTP by the complex nucleotide exchange factor, eIF2B. Bipartite motifs rich in aromatic and acidic residues are conserved at the C-termini of eIF5 and the catalytic (..
  20. ncbi Interaction of recombinant human eIF2 subunits with eIF2B and eIF2alpha kinases
    Rajasekhar N V S Suragani
    Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India
    Biochem Biophys Res Commun 338:1766-72. 2005
    ..Consistent with this observation, the beta-subunit specifically interacts with the purified eIF2B in ELISA studies and this interaction is enhanced when wt eIF2alpha in the recombinant trimeric complex is ..
  21. pmc Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2
    N T Price
    Department of Biochemistry, University of Bristol, U K
    Biochem J 318:631-6. 1996
    Peptide sequence data were obtained from rabbit protein synthesis initiation factor subunit eIF2B gamma...
  22. pmc Conserved sequences in the beta subunit of archaeal and eukaryal translation initiation factor 2 (eIF2), absent from eIF5, mediate interaction with eIF2gamma
    G M Thompson
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Botucatu, 862, Sao Paulo, 04023 062, Brasil
    Biochem J 347:703-9. 2000
    ..aIF2beta differs from eIF2beta in not possessing an N-terminal extension implicated in binding RNA, eIF5 and eIF2B. The remaining sequences are highly conserved, and are shared with eIF5...
  23. pmc eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Genes Dev 12:514-26. 1998
    b>eIF2B is a heteropentameric guanine-nucleotide exchange factor essential for protein synthesis initiation in eukaryotes...
  24. ncbi Biochemical analysis of the eIF2beta gamma complex reveals a structural function for eIF2alpha in catalyzed nucleotide exchange
    J Nika
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75083, USA
    J Biol Chem 276:1051-6. 2001
    ..GDP complex that is recycled to the GTP form by the nucleotide exchange factor eIF2B. The alpha-subunit of eIF2 plays a critical role in regulating nucleotide exchange via phosphorylation at serine ..
  25. ncbi Thermal injury impairs cardiac protein synthesis and is associated with alterations in translation initiation
    Charles H Lang
    Department of Cellular and Molecular Physiology H166, Penn State College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Regul Integr Comp Physiol 286:R740-50. 2004
    ..Burn failed to alter eIF2B activity or the total amount or phosphorylation status of either eIF2 alpha or eIF2B epsilon in heart...
  26. ncbi Direct binding of translation initiation factor eIF2gamma-G domain to its GTPase-activating and GDP-GTP exchange factors eIF5 and eIF2B epsilon
    Pankaj V Alone
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:12636-44. 2006
    ..The factor eIF5 stimulates hydrolysis of GTP by eIF2 upon AUG codon recognition, whereas the factor eIF2B promotes guanine nucleotide exchange on eIF2 to recycle the factor for additional rounds of translation initiation...
  27. ncbi Glycogen synthase kinase-3 is the predominant insulin-regulated eukaryotic initiation factor 2B kinase in skeletal muscle
    L S Jefferson
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey 17033, USA
    Int J Biochem Cell Biol 31:191-200. 1999
    Eukaryotic initiation factor eIF2B is a guanine nucleotide exchange protein involved in regulation of translation initiation. Phosphorylation of the epsilon-subunit is thought to be important in insulin-mediated changes in eIF2B activity...
  28. ncbi Developmental changes in the feeding-induced stimulation of translation initiation in muscle of neonatal pigs
    T A Davis
    Department of Pediatrics, United States Department of Agriculture Agricultural Research Service, Children s Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Endocrinol Metab 279:E1226-34. 2000
    ..Each of these feeding-induced responses was blunted in muscle of 26-day-old pigs. eIF2B activity in muscle was unaffected by feeding status but decreased with development...
  29. pmc A novel mechanism for the control of translation initiation by amino acids, mediated by phosphorylation of eukaryotic initiation factor 2B
    Xuemin Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Life Sciences Centre, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada
    Mol Cell Biol 28:1429-42. 2008
    Eukaryotic initiation factor 2B (eIF2B) plays a key role in controlling the initiation of mRNA translation. eIF2B is heteropentamer whose catalytic (epsilon) subunit promotes GDP/GTP exchange on eIF2...
  30. ncbi Reducing agents mitigate protein synthesis inhibition mediated by vanadate and vanadyl compounds in reticulocyte lysates
    T Krishnamoorthy
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, India
    Arch Biochem Biophys 349:122-8. 1998
    ..of the alpha (alpha) subunit of initiation factor 2 (eIF2) or decrease the guanine nucleotide exchange activity of eIF2B, which is required to exchange GDP for GTP in eIF2.GDP binary complex...
  31. pmc Reduced eukaryotic initiation factor 2Bepsilon-subunit expression suppresses the transformed phenotype of cells overexpressing the protein
    James W Gallagher
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Cancer Res 68:8752-60. 2008
    Eukaryotic initiation factor 2B (eIF2B), a five-subunit guanine nucleotide exchange factor, plays a key role in the regulation of mRNA translation...
  32. doi Crystal structure of the C-terminal domain of the ɛ subunit of human translation initiation factor eIF2B
    Jia Wei
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Protein Cell 1:595-603. 2010
    Eukaryotic translation initiation factor eIF2B, the guanine nucleotide exchange factor (GEF) for eIF2, catalyzes conversion of eIF2·GDP to eIF2·GTP...
  33. pmc Eukaryotic initiation factor 2B (eIF2B) GEF activity as a diagnostic tool for EIF2B-related disorders
    Laetitia Horzinski
    INSERM U931 CNRS 6247 Université Clermont, GReD, Clermont Ferrand, France
    PLoS ONE 4:e8318. 2009
    ..forms with an onset after 16 years old and restricted to slow cognitive impairment have lead to the concept of eIF2B-related disorders...
  34. ncbi eIF2 and the control of cell physiology
    Christopher G Proud
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dunde DD15EH, United Kingdom
    Semin Cell Dev Biol 16:3-12. 2005
    Eukaryotic initiation factor eIF2 and its 'exchange factor' eIF2B play a key role in the regulation of protein synthesis in eukaryotes from yeast to mammals. Phosphorylation of eIF2 inhibits eIF2B and thus translation initiation...
  35. ncbi Impaired protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4E in muscle and eIF2B in liver
    C H Lang
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033 0850, USA
    Alcohol Clin Exp Res 24:322-31. 2000
    ..Acute alcohol intoxication in rats decreases protein synthesis in skeletal muscle and, to a lesser extent, in liver. The purpose of the present study was to examine potential mechanisms for the inhibitory effect of acute ethanol exposure...
  36. ncbi Endotoxin-induced decrease in muscle protein synthesis is associated with changes in eIF2B, eIF4E, and IGF-I
    C H Lang
    Departments of Cellular and Molecular Physiology, and Surgery, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Endocrinol Metab 278:E1133-43. 2000
    ..The content of eIF2alpha or the amount of eIF2alpha in the phosphorylated form did not change in response to LPS. eIF2B activity was decreased in muscle 4 h post-LPS but activity returned to control values by 24 h...
  37. ncbi Eukaryotic initiation factors and protein synthesis after resistance exercise in rats
    P A Farrell
    Noll Physiological Research Center and Graduate Program in Physiology, Pennsylvania State University, University Park 16802, USA
    J Appl Physiol (1985) 88:1036-42. 2000
    ..J. Physiol. Endocrinol. Metab. 276: E721-E727, 1999) that increases in one factor, eIF2B, are associated with increases in rates of protein synthesis after resistance exercise in rats...
  38. ncbi Mammalian stress granules represent sites of accumulation of stalled translation initiation complexes
    Scot R Kimball
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Cell Physiol 284:C273-84. 2003
    ..fibroblasts that stress granules contain not only eIF2 but also the guanine nucleotide exchange factor for eIF2, eIF2B. Moreover, we show that phosphorylation of the alpha-subunit of eIF2 is necessary and sufficient for stress ..
  39. ncbi Phosphorylated seryl and threonyl, but not tyrosyl, residues are efficient specificity determinants for GSK-3beta and Shaggy
    D D Williams
    Department of Anatomy and Physiology, University of Dundee, UK
    FEBS Lett 448:86-90. 1999
    ..peptides derived from a putative glycogen synthase kinase-3 site in the Drosophila translation initiation factor eIF2B epsilon to investigate the efficacy of residues other than Ser(P) as priming residues for glycogen synthase kinase-..
  40. ncbi Chronic alcohol feeding impairs hepatic translation initiation by modulating eIF2 and eIF4E
    C H Lang
    Department of Cellular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol 277:E805-14. 1999
    ..Alcohol feeding decreased hepatic eIF2B activity by 36%...
  41. ncbi Regulation of protein synthesis by insulin
    C G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, Canada V6T 1Z3
    Biochem Soc Trans 34:213-6. 2006
    ..PKB acts to phosphorylate and inactivate glycogen synthase kinase 3, which in turn phosphorylates and inhibits eIF2B. Insulin elicits the dephosphorylation and activation of eIF2B...
  42. ncbi Interplay between insulin and nutrients in the regulation of translation factors
    C G Proud
    Division of Molecular Physiology, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Biochem Soc Trans 29:541-7. 2001
    ..Insulin activates several of these proteins including the following: the guanine-nucleotide exchange factor eIF2B; the eIF4F complex, which (through eIF4E) interacts with the cap of the mRNA; p70 S6 kinase; and elongation factor ..
  43. pmc Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation
    Edith Gomez
    Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester M60 1QD, UK
    EMBO J 21:5292-301. 2002
    For protein synthesis initiation in eukaryotes, eIF2B is the guanine-nucleotide exchange factor for eIF2. eIF2B is an essential multi-subunit factor and a major target for translational control in both yeast and mammalian cells...
  44. ncbi Initiation factor 2B activity is regulated by protein phosphatase 1, which is activated by the mitogen-activated protein kinase-dependent pathway in insulin-like growth factor 1-stimulated neuronal cells
    Celia Quevedo
    Servicio de Bioquímica Investigación, Hospital Ramon y Cajal, 28034 Madrid, Spain
    J Biol Chem 278:16579-86. 2003
    We have previously demonstrated that insulin-like growth factor 1 (IGF1) induces eukaryotic initiation factor 2B (eIF2B) activation in neuronal cells through the phosphatidylinositol 3 kinase/glycogen synthase kinase 3 pathway as well as ..
  45. ncbi Characterization of the initiation factor eIF2B and its regulation in Drosophila melanogaster
    D D Williams
    School of Life Sciences, Medical Sciences Institute Wellcome Trust Biocentre Complex, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 276:3733-42. 2001
    Eukaryotic initiation factor (eIF) 2B catalyzes a key regulatory step in the initiation of mRNA translation. eIF2B is well characterized in mammals and in yeast, although little is known about it in other eukaryotes...
  46. doi Differentiation of rat iPS cells and ES cells into granulosa cell-like cells in vitro
    Juan Zhang
    Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha 410083, China
    Acta Biochim Biophys Sin (Shanghai) 45:289-95. 2013
    ..to analyze the expression level of marker genes in POF, including BMP15, FMR1, FSHR, INHA, AMH, NOBOX, FOXO3, EIF2B, FIGLA, and GDF9...
  47. pmc Intra-axonal synthesis of eukaryotic translation initiation factors regulates local protein synthesis and axon growth in rat sympathetic neurons
    Amar N Kar
    Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892 1381, USA
    J Neurosci 33:7165-74. 2013
    ..hybridization and metabolic labeling, we show that the mRNAs encoding eukaryotic translation initiation factors eIF2B2 and eIF4G2 are present in the axons of rat sympathetic neurons and are locally translated...
  48. ncbi The effect of genotype on the natural history of eIF2B-related leukodystrophies
    A Fogli
    INSERM UMR 384, Faculte de Medecine, Clermont Ferrand, France
    Neurology 62:1509-17. 2004
    Recessive mutations in the five eucaryotic initiation factor 2B (eIF2B) subunits have been found in leukodystrophies of variable age at onset and severity.
  49. ncbi Leucine activates pancreatic translational machinery in rats and mice through mTOR independently of CCK and insulin
    Maria Dolors Sans
    Department of Cellular and Molecular Physiology, Penn State College of Medicine, Hershey, PA 17033, USA
    J Nutr 136:1792-9. 2006
    ..the initiation factors eIF4E and eIF4G, but did not affect the activity of the guanine nucleotide exchange factor eIF2B, nor total protein synthesis...
  50. ncbi Defective translational control facilitates vesicular stomatitis virus oncolysis
    Siddharth Balachandran
    Department of Microbiology and Immunology and Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, Miami, FL 33136, USA
    Cancer Cell 5:51-65. 2004
    ..Nevertheless, eIF2B-mediated guanine nucleotide exchange activity downstream of eIF2 was frequently aberrant in transformed cells, ..
  51. doi A viral factor, CpBV15α, interacts with a translation initiation factor, eIF2, to suppress host gene expression at a post-transcriptional level
    Rahul Hepat
    Department of Bioresource Sciences, Andong National University, Andong 760 749, Republic of Korea
    J Invertebr Pathol 114:34-41. 2013
    ..Immunoprecipitation (IP) of CpBV15α co-precipitated eIF2 and eIF2B (a guanine nucleotide exchange factor of eIF2) in parasitized P. xylostella...
  52. ncbi Assessment of biomarkers of protein anabolism in skeletal muscle during the life span of the rat: sarcopenia despite elevated protein synthesis
    Scot R Kimball
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    Am J Physiol Endocrinol Metab 287:E772-80. 2004
    ..in the binding of initiator methionyl-tRNA to the 40S ribosomal subunit, eukaryotic initiation factors (eIF)2 and eIF2B, as well as the 70-kDa ribosomal protein S6 kinase, S6K1, was lower at 6 mo compared with 1 mo of age...
  53. ncbi Non-eIF2B-related cystic leukoencephalopathy of unknown origin
    Marjo S van der Knaap
    Ann Neurol 59:724. 2006
  54. pmc Developmental splicing deregulation in leukodystrophies related to EIF2B mutations
    Aurélia Huyghe
    Genetique, reproduction et développement GReD, Faculte de Medecine, Clermont Ferrand, France
    PLoS ONE 7:e38264. 2012
    ..with cystic myelin breakdown including those related to mutations in the ubiquitous translation initiation factor eIF2B. EIF2B is involved in global protein synthesis and its regulation under normal and stress conditions...
  55. doi The binding mechanism of eIF2β with its partner proteins, eIF5 and eIF2Bε
    Zuoqi Gai
    Graduate School of Life Science, Faculty of Advanced Life Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochem Biophys Res Commun 423:515-9. 2012
    ..The inactive form eIF2-GDP is then exchanged for the active form eIF2-GTP by eIF2B for further initiation cycle...
  56. pmc DYRK2 negatively regulates cardiomyocyte growth by mediating repressor function of GSK-3β on eIF2Bε
    Celine S Weiss
    Internal Medicine III, University Hospital Heidelberg and DZHK German Center for Cardiovascular Research, Heidelberg, Germany
    PLoS ONE 8:e70848. 2013
    ..A central regulator of translation initiation is Eukaryotic initiation factor 2B (eIF2B)...
  57. pmc Dynamic cycling of eIF2 through a large eIF2B-containing cytoplasmic body: implications for translation control
    Susan G Campbell
    Faculty of Life Science, The University of Manchester, Manchester, M13 9PT, England, UK
    J Cell Biol 170:925-34. 2005
    The eukaryotic translation initiation factor 2B (eIF2B) provides a fundamental controlled point in the pathway of protein synthesis...
  58. ncbi Early MR imaging and spectroscopy appearance of eIF2B-related leukoencephalopathy
    M Mascalchi
    Radidodiagnostic Section, Department of Clinical Physiopathology, University of Florence, Viale Morgagni 85, 50134 Florence, Italy
    Neurology 67:537-8. 2006
  59. ncbi Vanishing white matter disease: a review with focus on its genetics
    Jan C Pronk
    Department of Human Genetics, VU University Medical Center, Amsterdam, The Netherlands
    Ment Retard Dev Disabil Res Rev 12:123-8. 2006
    ..The process of localization and identification of the first two genes related to VWM, EIF2B5 and EIF2B2, was facilitated by two founder effects in the Dutch population...
  60. ncbi Regulation of muscle protein synthesis in neonatal pigs during prolonged endotoxemia
    Renan A Orellana
    USDA ARS Children s Nutrition Research Center, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USA
    Pediatr Res 55:442-9. 2004
    ..In muscle and liver, LPS did not alter eIF2B activity, an event that regulates initiator met-tRNA(i) binding to the 40S ribosomal complex...
  61. ncbi Regulation of translation initiation by insulin and amino acids in skeletal muscle of neonatal pigs
    Pamela M J O'Connor
    United States Department of Agriculture Agricultural Research Service, Children s Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Endocrinol Metab 285:E40-53. 2003
    ..to eIF4E, increased eIF4E binding to eIF4G, and increased fractional protein synthesis rates but did not affect eIF2B activity...
  62. ncbi Membrane phospholipids and high-energy metabolites in childhood ataxia with CNS hypomyelination
    S Bluml
    Magnetic Resonance Spectroscopy Unit, Huntington Medical Research Institutes, Pasadena, CA 91105, USA
    Neurology 61:648-54. 2003
    Childhood ataxia with CNS hypomyelination (CACH) is a leukodystrophy with extreme rarefaction of white matter caused by mutations in one of the five subunits of the translation initiation factor 2B (eIF2B).
  63. ncbi [eIF2B and Cree Indian leukodystrophies]
    Anne Fogli
    Med Sci (Paris) 19:283-4. 2003
  64. doi Sensitivity and specificity of decreased CSF asialotransferrin for eIF2B-related disorder
    A Vanderver
    Children s National Medical Center, Children s Research Institute, Center for Genetic Medicine, Washington, DC 20010, USA
    Neurology 70:2226-32. 2008
    This is a study estimating diagnostic accuracy of CSF asialotransferrin to transferrin ratio measurement in eIF2B related disorders by using clinical evaluation and EIF2B mutation analysis as the reference standard...
  65. doi Intersubunit and interprotein interactions of alpha- and beta-subunits of human eIF2: Effect of phosphorylation
    Kamindla Rajesh
    Department of Biochemistry, University of Hyderabad, Gachibowli, Hyderabad 500 046, Andhra Pradesh, India
    Biochem Biophys Res Commun 374:336-40. 2008
    ..Recombinant phosphorylated human eIF2alpha that cannot interact with purified eIF2B, the GDP/GTP exchange factor of eIF2, however interacts efficiently with eIF2B along with the beta-subunit of eIF2 ..
  66. doi The ovarioleukodystrophy
    Stéphane Mathis
    University of Poitiers, CHU Poitiers, Department of Neurology, 2 rue de la Miletrie, 86021 Poitiers cedex 05, France
    Clin Neurol Neurosurg 110:1035-7. 2008
    ..have a variant of "vanishing white matter disease" with mutations in subunits of eukaryotic initiation factor 2B (EIF2B)...
  67. ncbi Impaired myocardial protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4F
    C H Lang
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Endocrinol Metab 279:E1029-38. 2000
    ..Acute alcohol intoxication did not significantly alter the myocardial content of eIF2 alpha or eIF2B epsilon, the extent of eIF2 alpha phosphorylation, or the activity of eIF2B...
  68. doi Identification of novel EIF2B mutations in Chinese patients with vanishing white matter disease
    Ye Wu
    Department of Pediatrics, Peking University First Hospital, Beijing, China
    J Hum Genet 54:74-7. 2009
    ..in EIF2B1-5 (identified in 2001-2002) encoding the five subunits of eukaryotic translation initiation factor (eIF2B alpha, beta, gamma, delta and epsilon), respectively...
  69. pmc Translation initiation factors are not required for Dicistroviridae IRES function in vivo
    Nilsa Deniz
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    RNA 15:932-46. 2009
    ..CrPV IGR IRES activity does not require the eukaryotic initiation factors eIF4G1 or eIF5B, and it is enhanced when eIF2B, the eIF3b subunit of eIF3, or eIF4E are impaired...
  70. pmc Archaeal aIF2B interacts with eukaryotic translation initiation factors eIF2alpha and eIF2Balpha: Implications for aIF2B function and eIF2B regulation
    Kamal Dev
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Mol Biol 392:701-22. 2009
    ..the alpha-subunit of eIF2 (eukaryotic initiation factor 2), which inhibits its guanine nucleotide exchange factor, eIF2B. The N-terminal S1 domain of phosphorylated eIF2alpha interacts with a subcomplex of eIF2B formed by the three ..
  71. doi Clues to the mechanism of action of eIF2B, the guanine-nucleotide-exchange factor for translation initiation
    Sarah S Mohammad-Qureshi
    Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester, UK
    Biochem Soc Trans 36:658-64. 2008
    ..eukaryotic translation initiation between eIF (eukaryotic initiation factor) 2, a G-protein, its five-subunit GEF, eIF2B, and its GAP, eIF5...
  72. doi Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics
    Keiji Kito
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan
    Proteomics 8:2366-70. 2008
    ..We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.
  73. pmc Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis
    Neil Kubica
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, United States
    Int J Biochem Cell Biol 40:2522-33. 2008
    ..and a stimulation of new eIF2Bepsilon protein synthesis, but had no effect on mRNAs encoding the other four eIF2B subunits...
  74. ncbi Effect of sepsis on eIE4E availability in skeletal muscle
    T C Vary
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
    Am J Physiol Endocrinol Metab 279:E1178-84. 2000
    ..Instead, on the basis of our previous studies, changes in eIF2B appear to be responsible for limiting protein synthesis in skeletal muscle during sepsis.
  75. doi Exon deletion in the non-catalytic domain of eIF2Bepsilon due to a splice site mutation leads to infantile forms of CACH/VWM with severe decrease of eIF2B GEF activity
    L Horzinski
    INSERM UMR384, Faculte de Medecine, 28 Place Henri Dunant, F 63003 Clermont Ferrand, France
    Ann Hum Genet 72:410-5. 2008
    ..mutations occurring in the five genes (EIF2B1-5) that encode the translation eukaryotic initiation factor eIF2B. We found that a patient with infantile CACH/VWM carries a mutation in the acceptor splice site of EIF2B5 exon 6...
  76. doi Genetic and clinical heterogeneity in eIF2B-related disorder
    Jelena Maletkovic
    Children s National Medical Center, Children s Research Institute, Center for Genetic Medicine, Washington, DC 20010, USA
    J Child Neurol 23:205-15. 2008
    Eukaryotic initiation factor 2B (eIF2B)-related disorders are heritable white matter disorders with a variable clinical phenotype (including vanishing white matter disease and ovarioleukodystrophy) and an equally heterogeneous genotype...
  77. ncbi Characterization of the mammalian initiation factor eIF2B complex as a GDP dissociation stimulator protein
    D D Williams
    School of Life Sciences, Medical Sciences Institute Wellcome Trust Biocentre Complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 276:24697-703. 2001
    Initiation factor eIF2B mediates a key regulatory step in the initiation of mRNA translation, i.e. the regeneration of active eIF2.GTP complexes. It is composed of five subunits, alpha-epsilon...
  78. ncbi Acute neurological deterioration in ovarioleukodystrophy related to EIF2B mutations: pregnancy with oocyte donation is a potentially precipitating factor
    L Peter
    CHU de Lyon, Service d Epileptologie, Hopital Neurologique, Lyon Cedex 3, France
    Eur J Neurol 15:94-7. 2008
    Mutations in the eukaryotic translation initiation factor 2B (eIF2B) represent a heterogenous group of autosomal recessive leucodystrophy characterized by a diffuse CSF-like aspect of the white matter at MRI designed as vanishing white ..
  79. pmc Change in nutritional status modulates the abundance of critical pre-initiation intermediate complexes during translation initiation in vivo
    Chingakham Ranjit Singh
    Molecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    J Mol Biol 370:315-30. 2007
    ..TC) interacts with eIF3-eIF1-eIF5 complex to form the multifactor complex (MFC), while eIF2GDP associates with eIF2B for guanine nucleotide exchange. Gcn2p phosphorylates eIF2 to inhibit eIF2B...
  80. doi Crystal structure of the alpha subunit of human translation initiation factor 2B
    Takuya B Hiyama
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Mol Biol 392:937-51. 2009
    Eukaryotic translation initiation factor 2B (eIF2B) is the heteropentameric guanine-nucleotide exchange factor specific for eukaryotic initiation factor 2 (eIF2)...
  81. ncbi [Eukaryotic translation initiation factor 2B and leukoencephalopathy with vanishing white matter]
    Yan Xia Pan
    Department of Pediatric, Peking University First Hospital, Beijing 100034, China
    Beijing Da Xue Xue Bao 41:608-10. 2009
    ..with the gene defects in EIF2B1-5, encoding the five subunits of eukaryotic translation initiation factor (eIF2B alpha, beta, gamma, delta and epsilon ) respectively...
  82. doi Protein synthesis and its control in neuronal cells with a focus on vanishing white matter disease
    Graham D Pavitt
    Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, UK
    Biochem Soc Trans 37:1298-310. 2009
    ..VWM/CACH is caused by mutations in the translation initiation factor, eIF2B, a component of the basal translational machinery in all cells.
  83. pmc eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast
    N T Price
    Department of Biochemistry, University of Bristol, U K
    Biochem J 318:637-43. 1996
    The guanine nucleotide-exchange factor eIF2B mediates the exchange of GDP bound to translation initiation factor eIF2 for GTP...
  84. ncbi Implication of eIF2B rather than eIF4E in the regulation of global protein synthesis by amino acids in L6 myoblasts
    S R Kimball
    Department of Cellular and Molecular Physiology, The Pennsylvania State University, College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 273:30945-53. 1998
    ..that both amino acids stimulate initiation and coordinately regulate the activity of eukaryotic initiation factor eIF2B. The changes in eIF2B activity could be explained in part by modulation of the phosphorylation state of the alpha-..
  85. ncbi Eukaryotic type translation initiation factor 2: structure-functional aspects
    E A Stolboushkina
    Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
    Biochemistry (Mosc) 76:283-94. 2011
    ..There are also new data on initiation factors eIF5 and eIF2B that directly interact with eIF2 and control its participation in nucleotide exchange.
  86. doi Epstein-Barr and other viral mimicry of autoantigens, myelin and vitamin D-related proteins and of EIF2B, the cause of vanishing white matter disease: massive mimicry of multiple sclerosis relevant proteins by the Synechococcus phage
    C J Carter
    PolygenicPathways, Saint Leonards on Sea, East Sussex, UK
    Immunopharmacol Immunotoxicol 34:21-35. 2012
    ..align with known autoantigens, other myelin and vitamin D-related proteins and the translation initiation factor EIF2B, which is implicated in vanishing white matter disease...
  87. ncbi Eukaryotic initiation factor eIF2
    S R Kimball
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033, USA
    Int J Biochem Cell Biol 31:25-9. 1999
    ..of GDP bound to eIF2 for GTP is a prerequisite to binding Met-tRNAi and is mediated by a second initiation factor, eIF2B. In what is probably the best-characterized mechanism for the regulation of mRNA translation, phosphorylation of ..
  88. doi Functional analysis of recently identified mutations in eukaryotic translation initiation factor 2Bɛ (eIF2Bɛ) identified in Chinese patients with vanishing white matter disease
    Xuerong Leng
    Pediatric Department, Peking University First Hospital, Beijing, China
    J Hum Genet 56:300-5. 2011
    ..Gene defects in each of the five subunits of eukaryotic translation initiation factor 2B (eIF2B α-ɛ) are responsible for the disease, although the mechanism of the pathogenesis is not well understood...
  89. pmc Simvastatin represses protein synthesis in the muscle-derived C₂C₁₂ cell line with a concomitant reduction in eukaryotic initiation factor 2B expression
    Alexander P Tuckow
    Dept of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 300:E564-70. 2011
    ..synthesis occurred with a concomitant decrease in expression and activity of eukaryotic initiation factor 2B (eIF2B), a regulated and rate-controlling guanine nucleotide exchange factor known to affect global rates of protein ..
  90. ncbi Inhibition of muscle protein synthesis by alcohol is associated with modulation of eIF2B and eIF4E
    C H Lang
    Departments of Cellular and Molecular Physiology and Surgery, Pennsylvania State College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol 277:E268-76. 1999
    ..Similarly, the relative amount of eIF2Bepsilon in muscle was also not different. In contrast, alcohol decreased eIF2B activity in psoas (fast-twitch) but not in soleus or heart (slow-twitch) muscles...
  91. pmc Inhibition of glycogen synthase kinase 3[beta] activity with lithium in vitro attenuates sepsis-induced changes in muscle protein turnover
    Stephen Bertsch
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA
    Shock 35:266-74. 2011
    ..the sepsis-induced increase in eukaryotic initiation factor (eIF) 2B[varepsilon] phosphorylation, decreased eIF2B activity, or the reduced phosphorylation of FOXO3, but instead was more closely associated with the continued ..
  92. ncbi Amino acid-induced stimulation of translation initiation in rat skeletal muscle
    T C Vary
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol 277:E1077-86. 1999
    ..Amino acid supplementation stimulated protein synthesis twofold. No changes were observed in eIF2B activity, in the amount of eIF4E associated with the eIF4E-binding protein (4E-BP1), or in the phosphorylation of ..
  93. pmc Regulation of the unfolded protein response by eif2Bdelta isoforms
    Leenus Martin
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 285:31944-53. 2010
    ..eIF2α phosphorylation inactivates the eIF2B complex...
  94. doi Roles of GSK3 in metabolic shift toward abnormal anabolism in cell senescence
    You Mie Kim
    Department of Biochemistry and Molecular Biology, The Graduate School, Ajou University, Suwon, Korea
    Ann N Y Acad Sci 1201:65-71. 2010
    ..Anabolism was accomplished through glycogen synthase, eIF2B, and SREBP1...
  95. pmc eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation
    Martin D Jennings
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Nature 465:378-81. 2010
    ..of the eIF2(alphaP) regulatory complex that inhibits the activity of the guanine-nucleotide exchange factor (GEF) eIF2B. Together our studies define a new step in the translation initiation pathway, one that is critical for normal ..
  96. pmc Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis
    Alexander P Tuckow
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 299:E241-8. 2010
    Eukaryotic initiation factor 2B (eIF2B) is a guanine nucleotide exchange factor (GEF) whose activity is both tightly regulated and rate-controlling with regard to global rates of protein synthesis...
  97. pmc Glucose and amino acids modulate translation factor activation by growth factors in PC12 cells
    M Kleijn
    Department of Anatomy and Physiology, MSI Wellcome Trust Building, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 347:399-406. 2000
    ..Glucose, but not amino acids, is required for the activation of eIF2B by EGF and NGF...
  98. ncbi A role for eukaryotic translation initiation factor 2B (eIF2B) in taste memory consolidation and in thermal control establishment during the critical period for sensory development
    Sharon Tirosh
    Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
    Dev Neurobiol 67:728-39. 2007
    ..It was established that eIF2Bepsilon-mRNA was induced in the preopotic anterior hypothalamus during heat conditioning. Taken together, these results correlate eIF2Bepsilon with sensory development...
  99. ncbi Leukoencephalopathy with vanishing white matter: from magnetic resonance imaging pattern to five genes
    Peter A J Leegwater
    Department of Child Neurology, Free University Medical Center, Amsterdam, The Netherlands
    J Child Neurol 18:639-45. 2003
    ..This gene encodes one of the five subunits of the translation factor eIF2B. Patients without mutations in the EIF2B5 gene were found to be mutated in one of the other genes that encode ..
  100. ncbi Vanishing white matter disease in a child presenting with ataxia
    C J Wilson
    National Metabolic Service, Starship Hospital, Auckland, New Zealand
    J Paediatr Child Health 41:65-7. 2005
    ..have been identified for the disease, EIF2B1-5, which encode the five subunits of translation initiation factor eIF2B. Mutations in each of the genes may independently cause the disease...
  101. pmc Evidence that the dephosphorylation of Ser(535) in the epsilon-subunit of eukaryotic initiation factor (eIF) 2B is insufficient for the activation of eIF2B by insulin
    Xuemin Wang
    Division of Molecular Physiology, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 367:475-81. 2002
    ..The largest (epsilon) subunit of eIF2B is a substrate for glycogen synthase kinase (GSK)-3 in vitro, and phosphorylation by GSK3 inhibits the activity of ..

Research Grants71

  1. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    Jeffrey Bonanno; Fiscal Year: 2002
    ..of an important subset of the remaining translation initiation factors [eIF4G, eIF4A, eIF2, IF2, eIF3, eIF1A, eIF2B, eIF5, the poly (A)-binding protein (PABP), and a PABP-interacting protein]...
  2. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..of an important subset of the remaining translation initiation factors [eIF4G, eIF4A, eIF2, IF2, eIF3, eIF1A, eIF2B, eIF5, the poly (A)-binding protein (PABP), and a PABP-interacting protein]...
  3. Amino Acids and Muscle Protein Synthesis
    Robert Wolfe; Fiscal Year: 2006
    ..to result in increased muscle protein synthesis unless there is sufficient charging of tRNA and activity of eIF2B. We propose that in the circumstance of an adequate basal amount of amino acids the transient stimulation of ..
  4. Cell biological analysis of CACH/VWM leukodystrophy
    Christoph Proschel; Fiscal Year: 2005
    ..has not been the case for the recently identified mutations in the subunits of translation initiation factor 2B (eIF2B) in patients diagnosed with autosomal recessive Childhood Ataxia with Diffuse CNS Hypomyelination (CACH)/Vanishing ..
  5. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    THOMAS VARY; Fiscal Year: 2002
    ..at two steps: formation of the 43S pre-initiation complex (controlled by eukaryotic initiation factor 2 (eIF2) and eIF2B); and the binding of mRNA to the 40S ribosome (controlled by elF4E)...
  6. REGULATION OF LIVER GROWTH AND FUNCTION
    Leonard Jefferson; Fiscal Year: 2004
    ..insulin, alter the synthesis of both total and specific hepatic routines via coordinated modulation of the eIF2/eIF2B, eIF4F and P70 S 6 K regulatory steps in translation initiation and the RNA and polymerase 1 step in ribosome bio ..
  7. MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATION
    Charles H Lang; Fiscal Year: 2010
    ..Overall, the research design will establish the mechanism by which myocardial protein synthesis is reduced in response to alcohol abuse. ..
  8. Cytokine Regulation of Muscle Protein Synthesis During Infection
    Charles Lang; Fiscal Year: 2009
    ..Our research will determine the mechanism for this muscle atrophy which will ultimately lead to development of novel therapeutic treatment and improve patient outcome. ..
  9. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  10. CYTOKINE REGULATION OF IGF SYSTEM DURING INFECTION
    Charles Lang; Fiscal Year: 2007
    ..abstract_text> ..
  11. Cytokine Regulation of Muscle Protein Synthesis During Infection
    Charles H Lang; Fiscal Year: 2010
    ..Our research will determine the mechanism for this muscle atrophy which will ultimately lead to development of novel therapeutic treatment and improve patient outcome. ..
  12. AntiretroviraI induced Defects in Muscle Protein Synthes
    Charles Lang; Fiscal Year: 2009
    ..Such data are needed to both realize the full potential and avoid possible pitfalls of this drug class in long-term treatment of HIV infection. ..
  13. Regulation of Nutrient Sensing and Muscle Wasting by Alcohol
    Charles Lang; Fiscal Year: 2009
    ..The proposed studies have a translational underpinning in that the data may lead to development of novel clinical interventions designed to reverse alcoholic myopathy during rehabilitation. ..
  14. GENETIC CONTROL OF SUSCEPTIBILITY TO TESTICULAR CANCER
    Joseph Nadeau; Fiscal Year: 2007
    ..modifier known, results from a mutation in the Deadend gene, (b) the TGCT suppressor at the Ay locus is Raly or Eif2b2 but not agouti, and also that the MGF enhancer is located in a 120 kb interval in which MGF is the only ..
  15. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles Lang; Fiscal Year: 2009
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  16. Regulation of Nutrient Sensing and Muscle Wasting by Alcohol
    Charles H Lang; Fiscal Year: 2010
    ..The proposed studies have a translational underpinning in that the data may lead to development of novel clinical interventions designed to reverse alcoholic myopathy during rehabilitation. ..
  17. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  18. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles Lang; Fiscal Year: 2007
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  19. Regulation of Nutrient Sensing and Muscle Wasting by Alcohol
    Charles Lang; Fiscal Year: 2007
    ..The proposed studies have a translational underpinning in that the data may lead to development of novel clinical interventions designed to reverse alcoholic myopathy during rehabilitation. ..
  20. Myocardial Protein Synthesis After Thermal Injury
    Charles Lang; Fiscal Year: 2005
    ..these changes are mediated by TNFa; (2) to determine the mechanism by which thermal injury decreases activity of eIF2B in heart, by assessing the phosphorylation of elF2Be, content of p67, and the importance of alterations in kinase ..
  21. REGULATION OF IGF SYSTEM AND MUSCLE WASTING BY ALCOHOL
    Charles Lang; Fiscal Year: 2001
    ....
  22. CYTOKINE REGULATION OF IGF SYSTEM DURING INFECTION
    Charles Lang; Fiscal Year: 1999
    ..These experiments will be conducted on cultured L6 myotubes, and protein synthesis, breakdown and amino acid transport will be quantitated. ..
  23. CYTOKINE REGULATION OF IGF SYSTEM DURING INFECTION
    Charles Lang; Fiscal Year: 2003
    ..abstract_text> ..
  24. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    Scot R Kimball; Fiscal Year: 2010
    ....
  25. INSULIN & PULSATILITY
    PETER FARRELL; Fiscal Year: 2000
    ..We will document whether part of the cause for increased insulin resistance in older persons is partially due to an altered insulin pulse profile. ..
  26. INSULIN & PULSATILITY
    PETER FARRELL; Fiscal Year: 2002
    ..We will document whether part of the cause for increased insulin resistance in older persons is partially due to an altered insulin pulse profile. ..