EEA1

Summary

Gene Symbol: EEA1
Description: early endosome antigen 1
Alias: MST105, MSTP105, ZFYVE2, early endosome antigen 1, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2
Species: human
Products:     EEA1

Top Publications

  1. Simonsen A, Lippe R, Christoforidis S, Gaullier J, Brech A, Callaghan J, et al. EEA1 links PI(3)K function to Rab5 regulation of endosome fusion. Nature. 1998;394:494-8 pubmed
    ..Here we identify the early-endosomal autoantigen EEA1 which binds the PI(3)K product phosphatidylinositol-3-phosphate, as a new Rab5 effector that is required for ..
  2. Kutateladze T, Ogburn K, Watson W, de Beer T, Emr S, Burd C, et al. Phosphatidylinositol 3-phosphate recognition by the FYVE domain. Mol Cell. 1999;3:805-11 pubmed
    ..PtdIns(3)P binding by the FYVE domain of human early endosome autoantigen 1 (EEA1), a protein implicated in endosome fusion, involves two beta hairpins and an alpha helix...
  3. McBride H, Rybin V, Murphy C, Giner A, Teasdale R, Zerial M. Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13. Cell. 1999;98:377-86 pubmed
    ..We now demonstrate that the Rab5 effectors EEA1 and Rabaptin-5/Rabex-5 exist on the membrane in high molecular weight oligomers, which also contain NSF...
  4. Christoforidis S, Zerial M. Purification and identification of novel Rab effectors using affinity chromatography. Methods. 2000;20:403-10 pubmed
    ..We expect this strategy to provide fundamental insights into the molecular mechanism of membrane transport but also to be applicable to several other GTPase-dependent biological functions. ..
  5. Pons V, Luyet P, Morel E, Abrami L, van der Goot F, Parton R, et al. Hrs and SNX3 functions in sorting and membrane invagination within multivesicular bodies. PLoS Biol. 2008;6:e214 pubmed publisher
    ..PtdIns3P thus controls the complementary functions of Hrs and SNX3 in sorting and multivesicular body biogenesis. ..
  6. Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, et al. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol Biol Cell. 2000;11:747-63 pubmed
    ..The early endosome antigen EEA1 was also redistributed to these aberrant membranes...
  7. Chang J, Lee S, Blackstone C. Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation. Proc Natl Acad Sci U S A. 2013;110:14954-9 pubmed publisher
    ..Protrudin also interacts with KIF5 and harbors a Rab-binding domain, a noncanonical FYVE (Fab-1, YGL023, Vps27, and EEA1) domain, and a two phenylalanines in an acidic tract (FFAT) domain and, thus, may also function in the distribution ..
  8. Chaubey P, Hofstetter L, Roschitzki B, Stieger B. Proteomic Analysis of the Rat Canalicular Membrane Reveals Expression of a Complex System of P4-ATPases in Liver. PLoS ONE. 2016;11:e0158033 pubmed publisher
    ..ATP11C at the bLPM and ATP11A in an intracellular vesicular compartment partially colocalizing with RAB7A and EEA1 as markers of the endosomal compartment...
  9. Takahashi K, Mashima H, Miura K, Maeda D, Goto A, Goto T, et al. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models. Sci Rep. 2017;7:2817 pubmed publisher
    ..In addition, larger autophagic vacuoles that colocalize with early endosome antigen 1 (EEA1) but not with lysosomal-associated membrane protein (LAMP)-1 were much more frequently formed in ..

More Information

Publications164 found, 100 shown here

  1. Inoue H, Matsuzaki Y, Tanaka A, Hosoi K, Ichimura K, Arasaki K, et al. γ-SNAP stimulates disassembly of endosomal SNARE complexes and regulates endocytic trafficking pathways. J Cell Sci. 2015;128:2781-94 pubmed publisher
    ..Moreover, the depletion partially suppressed the exit of EGFR and transferrin from EEA1-positive early endosomes to delay their degradation and uptake...
  2. Geumann U, Barysch S, Hoopmann P, Jahn R, Rizzoli S. SNARE function is not involved in early endosome docking. Mol Biol Cell. 2008;19:5327-37 pubmed publisher
    ..phosphatidyl inositol (PI)-3 phosphate, with the function of Rab-GTPases, as well as with early endosomal autoantigen 1 (EEA1), an essential tethering factor. We conclude that docking of early endosomes is independent of SNARE function.
  3. Sung Y, Winkler T, De Las Fuentes L, Bentley A, Brown M, Kraja A, et al. A Large-Scale Multi-ancestry Genome-wide Study Accounting for Smoking Behavior Identifies Multiple Significant Loci for Blood Pressure. Am J Hum Genet. 2018;102:375-400 pubmed publisher
    ..for biological candidates such as modulators of vascular structure and function (CDKN1B, BCAR1-CFDP1, PXDN, EEA1), ciliopathies (SDCCAG8, RPGRIP1L), telomere maintenance (TNKS, PINX1, AKTIP), and central dopaminergic signaling (..
  4. Furniss R, Slater S, Frankel G, Clements A. Enterohaemorrhagic E. coli modulates an ARF6:Rab35 signaling axis to prevent recycling endosome maturation during infection. J Mol Biol. 2016;428:3399-407 pubmed publisher
    ..uncoating of newly formed endosomes, and instead leads to the formation of enlarged EspG/TfR/Rab11 positive, EEA1/Clathrin negative stalled recycling structures...
  5. Das S, Lambright D. Membrane Trafficking: An Endosome Tether Meets a Rab and Collapses. Curr Biol. 2016;26:R927-R929 pubmed publisher
    ..A new study shows that Rab GTPase binding causes 'entropic collapse' of the coiled-coil endosome tether EEA1, driving membrane apposition and facilitating short-range interactions required for fusion.
  6. St Denis N, Gupta G, Lin Z, Gonzalez Badillo B, Pelletier L, Gingras A. Myotubularin-related proteins 3 and 4 interact with polo-like kinase 1 and centrosomal protein of 55 kDa to ensure proper abscission. Mol Cell Proteomics. 2015;14:946-60 pubmed publisher
    ..protein 3 and polo-like kinase 1 relies on the divergent, nonlipid binding Fab1, YOTB, Vac1, and EEA1 domain of myotubularin-related protein 3, and myotubularin-related protein 4 interacts with CEP55 through a short ..
  7. Jo Y, Lee I, Jung S, Kwon J, Kim N, Namgoong S. Spire localization via zinc finger-containing domain is crucial for the asymmetric division of mouse oocyte. FASEB J. 2019;33:4432-4447 pubmed publisher
    ..Spire is colocalized with zinc-containing vesicles via its zinc finger-containing Fab1, YOTB, Vac 1, EEA1 (FYVE) domain...
  8. Mohan R, Tripathi R, Sharma A, Sinha P, Giuliano E, Hesemann N, et al. Decorin antagonizes corneal fibroblast migration via caveolae-mediated endocytosis of epidermal growth factor receptor. Exp Eye Res. 2019;180:200-207 pubmed publisher
    ..Next, we found that Dcn-EGFR complex does not follow canonical early endosome internalization as revealed by the EEA1 antibody instead binds to the CD63 antibody directed for degradation by the late endosome...
  9. Snyder J, Rochelle L, Lyerly H, Caron M, BARAK L. Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi network. J Biol Chem. 2013;288:10286-97 pubmed publisher
    ..is dependent upon dynamin GTPase activity and find that wild-type full-length LGR5 rapidly internalizes into EEA1- and Rab5-positive endosomes...
  10. Cervantes J, La Vake C, Weinerman B, Luu S, O Connell C, Verardi P, et al. Human TLR8 is activated upon recognition of Borrelia burgdorferi RNA in the phagosome of human monocytes. J Leukoc Biol. 2013;94:1231-41 pubmed publisher
    ..Using confocal microscopy we provide evidence that TLR8 colocalizes with internalized Bb RNA in both early (EEA1) and late endosomes (LAMP1)...
  11. Bahnan W, Boettner D, Westermark L, Fällman M, Schesser K. Pathogenic Yersinia Promotes Its Survival by Creating an Acidic Fluid-Accessible Compartment on the Macrophage Surface. PLoS ONE. 2015;10:e0133298 pubmed publisher
    ..These Yersinia-containing acidic compartments (YACs) are bereft of the early endosomal marker EEA1 and the lysosomal antigen LAMP1 and readily form on primary macrophages as well as macrophage-like cell lines...
  12. Zhao P, Chen B, Li L, Wu H, Li Y, Shaneen B, et al. Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7. Biochim Biophys Acta Gen Subj. 2018;: pubmed publisher
    ..This new pathway may allow us to control the uptake of MNPs within cells by targeting MIM, thereby improving their medical applications. ..
  13. Gioria M, Pasini M, Berruti G. Dynamic of contribution of UBPy-sorted cargo to acrosome biogenesis: effects of its derailment in a mouse model of globozoospermia, the infertile Vps54 (L967Q) mutant. Cell Tissue Res. 2017;369:413-427 pubmed publisher
    ..The chosen proteins of this study were UBPy (ESCRT-DUB), together with endosome compartment markers EEA1 and pallidin. The latter is described here for the first time in male germ cells...
  14. Ito A, Fukaya M, Saegusa S, Kobayashi E, Sugawara T, Hara Y, et al. Pallidin is a novel interacting protein for cytohesin-2 and regulates the early endosomal pathway and dendritic formation in neurons. J Neurochem. 2018;147:153-177 pubmed publisher
    ..demonstrated that cytohesin-2 and pallidin partially colocalized in various subsets of endosomes immunopositive for EEA1, syntaxin 12, and LAMP2 in hippocampal neurons...
  15. Perini E, SCHAEFER R, Stöter M, Kalaidzidis Y, Zerial M. Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations. Traffic. 2014;15:1366-89 pubmed publisher
    ..the mammalian endocytic system, distinct subpopulations of early endosomes labelled by the Rab5 effectors APPL1 and EEA1 are present. Here, the function of mammalian CORVET with respect to these endosomal subpopulations was investigated...
  16. Canfrán Duque A, Barrio L, Lerma M, de la Peña G, Serna J, Pastor O, et al. First-Generation Antipsychotic Haloperidol Alters the Functionality of the Late Endosomal/Lysosomal Compartment in Vitro. Int J Mol Sci. 2016;17:404 pubmed publisher
    ..and the Golgi apparatus appeared to be unaffected by the antipsychotic as the distribution of both early endosome antigen 1 (EEA1) and coatomer subunit ? (?-COP) were not perturbed...
  17. Wilson J, de Hoop M, Zorzi N, Toh B, Dotti C, Parton R. EEA1, a tethering protein of the early sorting endosome, shows a polarized distribution in hippocampal neurons, epithelial cells, and fibroblasts. Mol Biol Cell. 2000;11:2657-71 pubmed
    b>EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes...
  18. Mani M, Lee U, Yoon N, Kim H, Ko M, Seol W, et al. Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling. Mol Biol Cell. 2016;27:334-48 pubmed publisher
    ..DRG2 was associated with phosphatidylinositol 3-phosphate-containing endosomes. It colocalized and interacted with EEA1 and Rab5 on endosomes in a phosphatidylinositol 3-kinase-dependent manner...
  19. Mu F, Callaghan J, Steele Mortimer O, Stenmark H, Parton R, Campbell P, et al. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. J Biol Chem. 1995;270:13503-11 pubmed
    ..We have cloned a human cDNA encoding an evolutionarily conserved 180-kDa protein on early endosomes named EEA1 (Early Endosome Antigen1)...
  20. Mukherjee J, Mahato B, Adhya S. Vesicular transport of a ribonucleoprotein to mitochondria. Biol Open. 2014;3:1083-91 pubmed publisher
    ..vesicles showed the sorting of the RNP away from Cav1 in a process that was independent of the endosomal effector EEA1 but sensitive to nocodazole...
  21. Reventun P, Alique M, Cuadrado I, Marquez S, Toro R, Zaragoza C, et al. iNOS-Derived Nitric Oxide Induces Integrin-Linked Kinase Endocytic Lysosome-Mediated Degradation in the Vascular Endothelium. Arterioscler Thromb Vasc Biol. 2017;37:1272-1281 pubmed publisher
    ..Likewise, ILK colocalized with the early endosome marker EEA1 (early endosome antigen 1)...
  22. English E, Mahn S, Marchese A. Endocytosis is required for CXC chemokine receptor type 4 (CXCR4)-mediated Akt activation and antiapoptotic signaling. J Biol Chem. 2018;293:11470-11480 pubmed publisher
    ..Here, we show that endocytosis and early endosome antigen 1 (EEA1), which is part of the endosome fusion machinery, are required for CXCL12-mediated AKT Ser/Thr ..
  23. Yap C, Digilio L, McMahon L, Garcia A, Winckler B. Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes. J Cell Biol. 2018;217:3141-3159 pubmed publisher
    ..We discovered a spatial heterogeneity of endolysosomal compartments in dendrites. Early EEA1-positive and late Rab7-positive endosomes are found throughout dendrites, whereas the density of degradative LAMP1- ..
  24. Chua C, Tang B. Engagement of the small GTPase Rab31 protein and its effector, early endosome antigen 1, is important for trafficking of the ligand-bound epidermal growth factor receptor from the early to the late endosome. J Biol Chem. 2014;289:12375-89 pubmed publisher
    ..that Rab31 is sequestered into a high molecular weight complex after stimulation with EGF, as was early endosome antigen 1 (EEA1), a factor responsible for endosomal tethering and fusion events...
  25. Wang Y, Chen X, Chen X, Chen Q, Huo K. Transcriptional profiling and dynamical regulation analysis identify potential kernel target genes of SCYL1-BP1 in HEK293T cells. Mol Cells. 2014;37:691-8 pubmed publisher
    ..Importantly, we showed the diminished SCYL1-BP1 dramatically reduced the expression levels of EEA1, BMPR2 and BRCA2 in HEK293T cells...
  26. Salova A, Belyaeva T, Leontieva E, Zlobina M, Kharchenko M, Kornilova E. Quantum dots implementation as a label for analysis of early stages of EGF receptor endocytosis: a comparative study on cultured cells. Oncotarget. 2016;7:6029-47 pubmed publisher
    ..the bEGF-savQD demonstrated similar to native EGF and bEGF-savCy3 co-localization dynamics with tethering protein EEA1 and HRS, the key component of sorting ESCRT0 complex...
  27. Chu J, Smelkinson M, Dorward D, Zarember K, Gallin J. Early Intracellular Trafficking of Granulibacter bethesdensis in Human Macrophages. Infect Immun. 2017;85: pubmed publisher
    ..We observed colocalization of G. bethesdensis with an early endosome antigen 1 (EEA1)-positive compartment, followed by colocalization with lysosome-associated membrane protein 1 (..
  28. Kolb C, Nagel M, Kalinowska K, Hagmann J, Ichikawa M, Anzenberger F, et al. FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis. Plant Physiol. 2015;167:1361-73 pubmed publisher
    ..VFD1 encodes a Fab1, YOTB, Vac1, and EEA1 (FYVE) domain-containing protein, FYVE1, that has been implicated in intracellular trafficking...
  29. Lee E, Park M, Kim H, Kim E, Kang G, Byun H, et al. Epithelial membrane protein 2 regulates sphingosylphosphorylcholine-induced keratin 8 phosphorylation and reorganization: Changes of PP2A expression by interaction with alpha4 and caveolin-1 in lung cancer cells. Biochim Biophys Acta. 2016;1863:1157-69 pubmed publisher
    ..SPC induced caveolin-1 (cav-1) expression and EEA1 endosome marker protein but not cav-2...
  30. Heidl S, Ellinger I, Niederberger V, Waltl E, Fuchs R. Localization of the human neonatal Fc receptor (FcRn) in human nasal epithelium. Protoplasma. 2016;253:1557-1564 pubmed
    ..Co-localization of FcRn α-chain with IgG or with early sorting endosomes (EEA1-positive) but not with late endosomes/lysosomes (LAMP-2-positive) in ciliated cells was observed...
  31. Liu Y, Zhuang J, Zhang X, Yue C, Zhu N, Yang L, et al. Autophagy associated cytotoxicity and cellular uptake mechanisms of bismuth nanoparticles in human kidney cells. Toxicol Lett. 2017;275:39-48 pubmed publisher
    ..Internalized BiNP did not co-localize with EEA1, but co-localized with Lysotracker/LAMP1/LAMP2 at late time points, indicating BiNP may be retained in the non-..
  32. Chen N, Chyau C, Lee Y, Tseng H, Chou F. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells. Sci Rep. 2016;6:20417 pubmed publisher
    ..Paclitaxel/MWE decreased EEA1 immunofluorescence staining and increased the expression of PTEN, indicating that the regimen inhibited the ..
  33. Jiang T, Pan C, Low B. BPGAP1 spatially integrates JNK/ERK signaling crosstalk in oncogenesis. Oncogene. 2017;36:3178-3192 pubmed publisher
    ..we found that the mRNA expression levels of both JNK1 and ERK2 are positively correlated with the mRNA level of EEA1, an endosome associated protein, indicating the potential JNK/ERK crosstalk at endosome...
  34. Henkels K, Miller T, Ganesan R, Wilkins B, Fite K, Gomez Cambronero J. A Phosphatidic Acid (PA) conveyor system of continuous intracellular transport from cell membrane to nucleus maintains EGF receptor homeostasis. Oncotarget. 2016;7:47002-47017 pubmed publisher
    ..D (PLD) causes a spike in intracellular PA production that forms vesicles transporting EGFR from early endosomes (EEA1 marker) and prolonged internalization in late endosomes and Golgi (RCAS marker)...
  35. Verma J, Rastogi R, Mukhopadhyay A. Leishmania donovani resides in modified early endosomes by upregulating Rab5a expression via the downregulation of miR-494. PLoS Pathog. 2017;13:e1006459 pubmed publisher
    ..Subsequently, L. donovani recruits and retains Rab5a and EEA1 on PV to reside in early endosomes and inhibits transport to lysosomes in human macrophages...
  36. Yoon H, Lee J, Randazzo P. ARAP1 regulates endocytosis of EGFR. Traffic. 2008;9:2236-52 pubmed publisher
    ..edge of the cell and punctate structures containing Rab5, rabaptin 5 and EGFR but not early embryonic antigen 1 (EEA1). EGF associated with the ARAP1-positive punctate structures prior to EEA1-positive early endosomes...
  37. Koumanov F, Pereira V, Whitley P, Holman G. GLUT4 traffic through an ESCRT-III-dependent sorting compartment in adipocytes. PLoS ONE. 2012;7:e44141 pubmed publisher
    ..compartment is produced in which GLUT4 co-localises more extensively with the endosomal markers including EEA1 and transferrin receptors but also with the TGN marker syntaxin6...
  38. Gallo L, Liao Y, Ruiz W, Clayton D, Li M, Liu Y, et al. TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. Mol Biol Cell. 2014;25:3779-97 pubmed publisher
    ..canine kidney cells, endogenous TBC1D9B colocalized with Rab11a-positive recycling endosomes but less so with EEA1-positive early endosomes, transferrin-positive recycling endosomes, or late endosomes...
  39. Shu Q, Lennemann N, Sarkar S, Sadovsky Y, Coyne C. ADAP2 Is an Interferon Stimulated Gene That Restricts RNA Virus Entry. PLoS Pathog. 2015;11:e1005150 pubmed publisher
    ..and that ADAP2 strongly associates with actin-enriched membrane ruffles and with Rab8a- and LAMP1-, but not EEA1- or Rab7-, positive vesicles...
  40. Gosney J, Wilkey D, Merchant M, Ceresa B. Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner. J Biol Chem. 2018;293:5895-5908 pubmed publisher
    ..The gradient was fractionated, and early endosomal fractions were pooled and immunoisolated with an EEA1 mAb...
  41. Niazy N, Temme S, Bocuk D, Giesen C, König A, Temme N, et al. Misdirection of endosomal trafficking mediated by herpes simplex virus-encoded glycoprotein B. FASEB J. 2017;31:1650-1667 pubmed publisher
    ..expression of gB induces giant vesicles of early endosomal origin, which contained Rab5, early endosomal antigen 1 (EEA1), and large amounts of MHC-II molecules [human leukocyte antigen (HLA)-DR, and HLA-DM], but no CD63...
  42. Ramm G, Slot J, James D, Stoorvogel W. Insulin recruits GLUT4 from specialized VAMP2-carrying vesicles as well as from the dynamic endosomal/trans-Golgi network in rat adipocytes. Mol Biol Cell. 2000;11:4079-91 pubmed
    ..positive vesicles also contained IRAP (88%) and the v-SNARE, VAMP2 (57%) but not markers of sorting endosomes (EEA1), late endosomes, or lysosomes (lgp120)...
  43. Lasiecka Z, Yap C, Katz J, Winckler B. Maturational conversion of dendritic early endosomes and their roles in L1-mediated axon growth. J Neurosci. 2014;34:14633-43 pubmed publisher
    ..these neuronal proteins interface with canonical endosomes and ubiquitously expressed endosomal components, such as EEA1 (Early Endosomal Antigen 1)...
  44. Kumar A, Dumasia K, Gaonkar R, Sonawane S, Kadam L, Balasinor N. Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation. Mol Cell Endocrinol. 2015;404:91-101 pubmed publisher
    ..with deregulation of genes involved in cytoskeleton remodeling (Arpc1b, Evl and Capg) and endocytosis (Picalm, Eea1 and Stx5a)...
  45. Oliveira Nunes M, Assad Kahn S, de Oliveira Barbeitas A, E Spohr T, Dubois L, Ventura Matioszek G, et al. The availability of the embryonic TGF-? protein Nodal is dynamically regulated during glioblastoma multiforme tumorigenesis. Cancer Cell Int. 2016;16:46 pubmed publisher
    ..and more differentiated GBM cells through morphological analysis, immunofluorescence of Nodal protein and of early (EEA1 and Rab5) and late (Rab7 and Rab11) endocytic markers and Western Blot...
  46. Compton L, Ikonomov O, Sbrissa D, Garg P, Shisheva A. Active vacuolar H+ ATPase and functional cycle of Rab5 are required for the vacuolation defect triggered by PtdIns(3,5)P2 loss under PIKfyve or Vps34 deficiency. Am J Physiol Cell Physiol. 2016;311:C366-77 pubmed publisher
    ..Bafilomycin A1 also restored the aberrant EEA1-positive endosomes, enlarged upon short PIKfyve inhibition with YM201636...
  47. Sánchez E, Pérez Núñez D, Revilla Y. Mechanisms of Entry and Endosomal Pathway of African Swine Fever Virus. Vaccines (Basel). 2017;5: pubmed publisher
    ..capsid and inner envelope are found in early endosomes or macropinosomes early after infection, colocalizing with EEA1 and Rab5, while at later times they co-localize with markers of late endosomes and lysosomes, such as Rab7 or Lamp ..
  48. Kauppi M, Simonsen A, Bremnes B, Vieira A, Callaghan J, Stenmark H, et al. The small GTPase Rab22 interacts with EEA1 and controls endosomal membrane trafficking. J Cell Sci. 2002;115:899-911 pubmed
    ..Rab22a caused formation of abnormally large vacuole-like structures containing the early-endosomal antigen EEA1 but not Rab11, a marker of recycling endosomes or the late-endosomal/lysosomal markers LAMP-1 and lyso-bis-..
  49. Chen P, Luo R, Jian X, Randazzo P. The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic. J Biol Chem. 2014;289:30237-48 pubmed publisher
    ..homology (PH) domain, phosphotyrosine-binding (PTB) domain, and leucine zipper motif (APPL)-positive endosomes and EEA1-positive endosomes was affected by ARAP2 knockdown and depended on ARAP2 GAP activity...
  50. Han Z, Lv M, Shi Y, Yu J, Niu J, Yu X, et al. Mutation of Glycosylation Sites in BST-2 Leads to Its Accumulation at Intracellular CD63-Positive Vesicles without Affecting Its Antiviral Activity against Multivesicular Body-Targeted HIV-1 and Hepatitis B Virus. Viruses. 2016;8:62 pubmed publisher
    ..of multivesicular body-targeted HIV-1 and hepatitis B virus, without affecting the co-localization of BST-2 with EEA1 and LAMP1...
  51. Clifford R, Maryon E, Kaplan J. Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cu⁺ uptake system. J Cell Sci. 2016;129:1711-21 pubmed publisher
    ..show that internalized CTR1 accumulates in early sorting endosomes and recycling compartments (containing Rab5 and EEA1), but not in late endosomes or lysosomal pathways...
  52. Wang M, Zhao D, Yang Y, Liu J, Wang J, Yin X, et al. The cellular prion protein negatively regulates phagocytosis and cytokine expression in murine bone marrow-derived macrophages. PLoS ONE. 2014;9:e102785 pubmed publisher
    ..Knockout of PrP(C) promoted bacterial uptake; upregulated Rab5, Rab7, and Eea1 mRNA expression; and increased the recruitment of lysosomal-associated membrane protein-2 to phagosomes, suggesting ..
  53. Gaullier J, Ronning E, Gillooly D, Stenmark H. Interaction of the EEA1 FYVE finger with phosphatidylinositol 3-phosphate and early endosomes. Role of conserved residues. J Biol Chem. 2000;275:24595-600 pubmed
    ..have investigated the structural requirements for the interaction of the FYVE finger of the early endosome antigen EEA1 with PtdIns(3)P and early endosomes...
  54. Mukai A, Mizuno E, Kobayashi K, Matsumoto M, Nakayama K, Kitamura N, et al. Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis. J Cell Sci. 2008;121:1325-33 pubmed publisher
    ..Our results thus implicate the ubiquitylation/deubiquitylation of proteins including VAMP8 in cytokinesis. ..
  55. Qi Y, Marlin M, Liang Z, Berry W, Janknecht R, Zhou J, et al. Distinct biochemical and functional properties of two Rab5 homologs from the rice blast fungus Magnaporthe oryzae. J Biol Chem. 2014;289:28299-309 pubmed publisher
    ..Like mammalian Rab5, MoRab5A and MoRab5B can bind to several Rab5 effectors in a GTP-dependent manner, including EEA1, Rabenosyn-5, and Rabaptin-5...
  56. Flores Rodriguez N, Kenwright D, Chung P, Harrison A, Stefani F, Waigh T, et al. ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome. J Cell Sci. 2015;128:755-67 pubmed publisher
    ..acts solely upon EGFR that has already entered the vacuolar early endosome (characterised by the presence of EEA1) or engages EGFR within earlier compartments...
  57. Kalaidzidis I, Miaczynska M, Brewińska Olchowik M, Hupalowska A, Ferguson C, Parton R, et al. APPL endosomes are not obligatory endocytic intermediates but act as stable cargo-sorting compartments. J Cell Biol. 2015;211:123-44 pubmed publisher
    ..a series of specialized endosomal compartments, beginning with early endosomes harboring Rab5 and its effector EEA1. There are, however, additional structures labeled by the Rab5 effector APPL1 whose role in endocytic transport ..
  58. Adams A, Wayne Vogl A. High Resolution Localization of Rab5, EEA1, and Nectin-3 to Tubulobulbar Complexes in the Rat Testis. Anat Rec (Hoboken). 2017;300:1160-1170 pubmed publisher
    ..Previous immunofluorescence studies have demonstrated that markers for early endosomes (Rab5 and EEA1) are associated with tubulobulbar complexes and are localized at or near the ends of the structures...
  59. Balogh P, Magyar M, Szabó A, Müllner N, Likó I, Patócs A, et al. The subcellular compartmentalization of TGFβ-RII and the dynamics of endosomal formation during the signaling events: An in vivo study on rat mesothelial cells. Eur J Cell Biol. 2015;94:204-13 pubmed publisher
    ..Using immunocytochemical approach, we could detect TβRII in EEA1 positive compartments and as the inflammation progressed, at D3, the receptor appeared in caveolin-1 positive ..
  60. Sandgren K, Smed Sörensen A, Forsell M, Soldemo M, Adams W, Liang F, et al. Human plasmacytoid dendritic cells efficiently capture HIV-1 envelope glycoproteins via CD4 for antigen presentation. J Immunol. 2013;191:60-9 pubmed publisher
    ..CD4-mediated uptake delivered Env to EEA1(+) endosomes that progressed to Lamp1(+) and MHC class II(+) lysosomes where internalized Env was degraded rapidly...
  61. McKnight N, Zhong Y, Wold M, Gong S, Phillips G, Dou Z, et al. Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex. PLoS Genet. 2014;10:e1004626 pubmed publisher
    ..We find that neuronal beclin 1 associates with endosomes and regulates EEA1/early endosome localization and late endosome formation...
  62. Jensen T, Cheema M, Szymiczek A, Damkier H, Praetorius J. Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance. PLoS ONE. 2015;10:e0124902 pubmed publisher
    ..Albumin did not colocalize with markers for early endosomes (EEA1), late endosomes/lysosomes (cathepsin D) or recycling endosomes (Rab11)...
  63. Venè R, Tosetti F, Minghelli S, Poggi A, Ferrari N, Benelli R. Celecoxib increases EGF signaling in colon tumor associated fibroblasts, modulating EGFR expression and degradation. Oncotarget. 2015;6:12310-25 pubmed
    ..This delay corresponded to a deferred dissociation of EEA1 from EGFR positive endosomes and the accumulation of Rab7, pro Cathepsin-D and SQSTM1/p62, suggesting a shared ..
  64. Faller E, Ghazawi F, Cavar M, MacPherson P. IL-7 induces clathrin-mediated endocytosis of CD127 and subsequent degradation by the proteasome in primary human CD8 T cells. Immunol Cell Biol. 2016;94:196-207 pubmed publisher
    ..and within 15-30?min is seen in multiple intracellular punctae co-localizing with the early endosomal marker EEA1. By 2?h after addition of IL-7, CD127 staining associates with the late endosomal marker RAB7 and with the ..
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    ..In K8-null hepatocytes, IR trafficking vesicles that are labeled by Rab5/EEA1/phosphatidylinositol 3-phosphate accumulate at a juxtanuclear region via a microtubule-dependent process...
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    ..pathway, in particular the rapid recycling pathway, shown by an increase in early endosomal antigen-1 positive (EEA1+), Rab4+, Rab11+ , and Rab7+ vesicles...
  67. Yokogawa M, Kobashigawa Y, Yoshida N, Ogura K, Harada K, Inagaki F. NMR analyses of the interaction between the FYVE domain of early endosome antigen 1 (EEA1) and phosphoinositide embedded in a lipid bilayer. J Biol Chem. 2012;287:34936-45 pubmed publisher
    ..the residue-specific level between PI-binding effector protein and PI-nanodisc, using the FYVE domain of early endosome antigen 1 (EEA1), denoted as EEA1 FYVE, and PI(3)P-nanodisc as a model system...
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    ..VAMP8-mediated secretion is dependent on expression of the early endosomal proteins Rab5, D52, and EEA1. Supramaximal CCK-8 (60 min) caused a 60% reduction in the expression of D52 followed by Rab5 and EEA1 in isolated ..
  69. Svensson L, Stanley P, Willenbrock F, Hogg N. The Gαq/11 proteins contribute to T lymphocyte migration by promoting turnover of integrin LFA-1 through recycling. PLoS ONE. 2012;7:e38517 pubmed publisher
    ..Additionally Gαq co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles...
  70. Johnson I, Parkinson Lawrence E, Keegan H, Spillane C, Barry O Crowley J, Watson W, et al. Endosomal gene expression: a new indicator for prostate cancer patient prognosis?. Oncotarget. 2015;6:37919-29 pubmed publisher
    ..The expression of APPL1, RAB5A, EEA1, PDCD6IP, NOX4 and SORT1 were altered in malignant patient tissue, when compared to indolent and normal prostate ..
  71. Nguyen O, Grimm C, Schneider L, Chao Y, Atzberger C, Bartel K, et al. Two-Pore Channel Function Is Crucial for the Migration of Invasive Cancer Cells. Cancer Res. 2017;77:1427-1438 pubmed publisher
    ..Disrupting TPC function halted trafficking of β1-integrin, leading to its accumulation in EEA1-positive early endosomes...
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    ..Expression of the genes CYCS, EEA1, SLC16A7, MEPCE, and TFB2M was validated in three new pairs of biological replicates of embryos...
  73. Ramanathan H, Ye Y. The p97 ATPase associates with EEA1 to regulate the size of early endosomes. Cell Res. 2012;22:346-59 pubmed publisher
    ..we identify a novel function of p97 in endocytic trafficking by establishing the early endosomal autoantigen 1 (EEA1) as a new p97 substrate...
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    ..We also found that Rab5 and EEA1 were accumulated in membranes by GFP-Rab11-FIP1A but Rab4 was unaffected, suggesting that a direct pathway may ..
  75. Pons V, Ustunel C, Rolland C, Torti E, Parton R, Gruenberg J. SNX12 role in endosome membrane transport. PLoS ONE. 2012;7:e38949 pubmed publisher
    ..Altogether, our data show that despite lower expression level, SNX12 shares redundant functions with SNX3 in the biogenesis of multivesicular endosomes. ..
  76. Ashjaei K, Palmberger D, Bublin M, Bajna E, Breiteneder H, Grabherr R, et al. Atopic donor status does not influence the uptake of the major grass pollen allergen, Phl p 5, by dendritic cells. J Immunol Methods. 2015;424:120-30 pubmed publisher
    ..of the allergens in early (EE) and late endosomes (LE) was detected by co-staining for early endosome antigen (EEA1) and lysosomal-associated membrane protein 1 (LAMP1)...
  77. Chrifi I, Louzao Martinez L, Brandt M, van Dijk C, Burgisser P, Zhu C, et al. CMTM3 (CKLF-Like Marvel Transmembrane Domain 3) Mediates Angiogenesis by Regulating Cell Surface Availability of VE-Cadherin in Endothelial Adherens Junctions. Arterioscler Thromb Vasc Biol. 2017;37:1098-1114 pubmed publisher
    ..Intracellular staining demonstrated that CMTM3 colocalizes with early endosome markers EEA1 (early endosome marker 1) and Clathrin+ vesicles and with cytosolic VE-cadherin in human umbilical vein ..
  78. Allan B, Moyer B, Balch W. Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science. 2000;289:444-8 pubmed
    ..We propose that Rab1-regulated assembly of functional effector-SNARE complexes defines a conserved molecular mechanism to coordinate recognition between subcellular compartments. ..
  79. Tanaka D, Nagashima K, Sasaki M, Funakoshi S, Kondo Y, Yasuda K, et al. Exome sequencing identifies a new candidate mutation for susceptibility to diabetes in a family with highly aggregated type 2 diabetes. Mol Genet Metab. 2013;109:112-7 pubmed publisher
    ..more frequent in the diabetes subjects than in the normal subjects was the N1072K variant of the early endosome antigen 1 (EEA1) gene (0 in normal subjects and 4 in diabetes subjects, p=0.022)...
  80. Castagnola P, Bellese G, Birocchi F, Gagliani M, Tacchetti C, Cortese K. Identification of an HSP90 modulated multi-step process for ERBB2 degradation in breast cancer cells. Oncotarget. 2016;7:85411-85429 pubmed publisher
    ..of the full-length p185-ERBB2, and promotes its cleavage, with the formation of a p116-ERBB2 form in EEA1-positive endosomes (EE)...
  81. Yuan W, Liu B, Wang X, Li T, Xue H, Mo X, et al. CMTM3 decreases EGFR expression and EGF-mediated tumorigenicity by promoting Rab5 activity in gastric cancer. Cancer Lett. 2017;386:77-86 pubmed publisher
    ..CMTM3 colocalizes with early endosome markers Rab5 and EEA1. Co-immunoprecipitation (Co-IP) assay further confirms that CMTM3 interacts with Rab5...
  82. Connan C, Voillequin M, Chavez C, Mazuet C, Leveque C, Vitry S, et al. Botulinum neurotoxin type B uses a distinct entry pathway mediated by CDC42 into intestinal cells versus neuronal cells. Cell Microbiol. 2017;19: pubmed publisher
    ..Dynasore, a dynamin inhibitor, and reaches a common early endosomal compartment labeled by early endosome antigen (EEA1). In contrast to neuronal cells, HcB uses a Cdc42-dependent pathway to enter intestinal cells...
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    ..GLT-1-RFP was co-localized to the early endosome marker-EEA1 in astrocytes treated with the PPAR? agonists...
  84. Ahtiainen L, Luiro K, Kauppi M, Tyynelä J, Kopra O, Jalanko A. Palmitoyl protein thioesterase 1 (PPT1) deficiency causes endocytic defects connected to abnormal saposin processing. Exp Cell Res. 2006;312:1540-53 pubmed
    ..These data provide new insights into the metabolism of PPT1-deficient cells and offer a basis for further studies on cellular processes causing neuronal death in INCL and other neurodegenerative diseases. ..
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    ..PI3KC2? in regulating physiologically relevant dynamin-independent internalization pathways by recruiting early endosome antigen 1 (EEA1) to vesicular compartments, a step required for the intracellular trafficking of vesicles generated ..
  86. Villella V, Esposito S, Bruscia E, Vicinanza M, Cenci S, Guido S, et al. Disease-relevant proteostasis regulation of cystic fibrosis transmembrane conductance regulator. Cell Death Differ. 2013;20:1101-15 pubmed publisher
    ..CFTR depletion decreased the recruitment of the Rab5 effector early endosome antigen 1 to endosomes, thus reducing the local generation of phosphatidylinositol-3-phosphate...
  87. Rollason R, Dunstan K, Billcliff P, Bishop P, Gleeson P, Wise H, et al. Expression of HIV-1 Vpu leads to loss of the viral restriction factor CD317/Tetherin from lipid rafts and its enhanced lysosomal degradation. PLoS ONE. 2013;8:e75680 pubmed publisher
    ..Our studies underlie the importance of studying endogenous tetherin and let us propose a model in which Vpu intercepts newly internalised tetherin and diverts it for lysosomal destruction rather than recycling to the cell surface. ..
  88. Dutta S, Roy S, Polavaram N, Stanton M, Zhang H, Bhola T, et al. Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology. Cancer Res. 2016;76:418-28 pubmed publisher
    ..We found that NRP2 depletion in human prostate and pancreatic cancer cells resulted in the accumulation of EEA1/Rab5-positive early endosomes concomitant with a decrease in Rab7-positive late endosomes, suggesting a delay in ..
  89. Pires E, D Souza R, Needham M, Herr A, Jazaeri A, Li H, et al. Membrane associated cancer-oocyte neoantigen SAS1B/ovastacin is a candidate immunotherapeutic target for uterine tumors. Oncotarget. 2015;6:30194-211 pubmed publisher
    ..cell line, SNU539, expressed cell surface SAS1B that, after binding polyclonal antibodies, internalized into EEA1/LAMP1-positive early and late endosomes...
  90. Zhou Y, Howell F, Glebov O, Albrecht D, Williams G, Doherty P. Regulated endosomal trafficking of Diacylglycerol lipase alpha (DAGLα) generates distinct cellular pools; implications for endocannabinoid signaling. Mol Cell Neurosci. 2016;76:76-86 pubmed publisher
    ..A similar cycling is seen in COS-7 cells with the internalized DAGLα initially transported to EEA1 and Rab5-positive early endosomes via a clathrin-independent pathway before being transported back to the cell ..
  91. Ly S, Navaroli D, Didiot M, CARDIA J, Pandarinathan L, Alterman J, et al. Visualization of self-delivering hydrophobically modified siRNA cellular internalization. Nucleic Acids Res. 2017;45:15-25 pubmed publisher
    ..Likewise, preferential sd-rxRNA co-localization was demonstrated with EEA1 but not RBSN-containing endosomes, consistent with preferential EGF-like trafficking through EEA1-containing ..
  92. Moyers J, Volk C, Cao J, Zhang C, Ding L, Kiselyov V, et al. Internalization and localization of basal insulin peglispro in cells. Mol Cell Endocrinol. 2017;454:23-38 pubmed publisher
    ..Co-localization studies using antibodies to either insulin or PEG, and the early endosomal marker EEA1 showed that the overall internalization and subcellular localization pattern of BIL was similar to that of human ..