Gene Symbol: DYNLT1
Description: dynein light chain Tctex-type 1
Alias: CW-1, TCTEL1, tctex-1, dynein light chain Tctex-type 1, T-complex testis-specific protein 1 homolog, t-complex-associated-testis-expressed 1-like 1
Species: human
Products:     DYNLT1

Top Publications

  1. Palmer K, MacCarthy Morrogh L, Smyllie N, Stephens D. A role for Tctex-1 (DYNLT1) in controlling primary cilium length. Eur J Cell Biol. 2011;90:865-71 pubmed publisher
    ..Compared to individual depletions, double siRNA depletion of DHC2 and Tctex-1 causes an even greater increase in cilia length. Our data show that Tctex-1 is a key regulator of cilia length and most likely functions as part of dynein-2. ..
  2. King S, Dillman J, Benashski S, Lye R, Patel King R, Pfister K. The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein. J Biol Chem. 1996;271:32281-7 pubmed
  3. Nagano F, Orita S, Sasaki T, Naito A, Sakaguchi G, Maeda M, et al. Interaction of Doc2 with tctex-1, a light chain of cytoplasmic dynein. Implication in dynein-dependent vesicle transport. J Biol Chem. 1998;273:30065-8 pubmed
    ..Because dynein is a minus-end-directed microtubule-based motor protein, these results suggest that Doc2, especially Doc2beta, plays a role in dynein-dependent intracellular vesicle transport. ..
  4. Pfister K, Fisher E, Gibbons I, Hays T, Holzbaur E, McIntosh J, et al. Cytoplasmic dynein nomenclature. J Cell Biol. 2005;171:411-3 pubmed
    ..This nomenclature recognizes the two distinct cytoplasmic dynein complexes and has the flexibility to accommodate the discovery of new subunits and isoforms. ..
  5. Chuang J, Yeh T, Bollati F, Conde C, Canavosio F, Caceres A, et al. The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth. Dev Cell. 2005;9:75-86 pubmed
    ..Finally, our data suggest that the selective high level of Tctex-1 at the growth cone of growing axons drives fast neurite extension by modulating actin dynamics and also Rac1 activity. ..
  6. Schwarzer C, Barnikol Watanabe S, Thinnes F, Hilschmann N. Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74. Int J Biochem Cell Biol. 2002;34:1059-70 pubmed
  7. Nadano D, Nakayama J, Matsuzawa S, Sato T, Matsuda T, Fukuda M. Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton. Biochem J. 2002;364:669-77 pubmed
    ..As Tctex-1 is a component of a MT-based molecular motor, these results suggest that tastin plays an important role in mammalian cells by associating with the microtubular cytoskeleton. ..
  8. Machado R, Rudarakanchana N, Atkinson C, Flanagan J, Harrison R, Morrell N, et al. Functional interaction between BMPR-II and Tctex-1, a light chain of Dynein, is isoform-specific and disrupted by mutations underlying primary pulmonary hypertension. Hum Mol Genet. 2003;12:3277-86 pubmed
    ..Taken together, these data demonstrate a discrete function for the cytoplasmic domain of BMPR-II and justify further investigation of whether the interaction with and phosphorylation of Tctex-1 contributes to the pathogenesis of PPH. ..
  9. Hamada Y, Tsurumi Y, Nozaki S, Katoh Y, Nakayama K. Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. Mol Biol Cell. 2018;29:1628-1639 pubmed publisher
    ..the dynein-2 subunits, including previously undefined interactions, such as that between WDR60 and the TCTEX1D2-DYNLT1/DYNLT3 dimer...

More Information


  1. Varma D, Dawn A, Ghosh Roy A, Weil S, Ori McKenney K, Zhao Y, et al. Development and application of in vivo molecular traps reveals that dynein light chain occupancy differentially affects dynein-mediated processes. Proc Natl Acad Sci U S A. 2010;107:3493-8 pubmed publisher
    ..specificity and efficacy of these inducible, dimeric "traps" for the dynein light chains LC8 (Dynll1) and TcTex1 (Dynlt1)...
  2. Ismail O, Sriranganathan S, Zhang X, Bonventre J, Zervos A, Gunaratnam L. Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis. J Cell Physiol. 2018;233:6877-6895 pubmed publisher
    ..Surprisingly, expression of Tctex-1 T94E did not influence KIM-1-mediated efferocytosis. Our studies uncover a previously unknown role for Tctex-1 in KIM-1-dependent efferocytosis in epithelial cells. ..
  3. Jayappa K, Ao Z, Wang X, Mouland A, Shekhar S, Yang X, et al. Human immunodeficiency virus type 1 employs the cellular dynein light chain 1 protein for reverse transcription through interaction with its integrase protein. J Virol. 2015;89:3497-511 pubmed publisher
    ..for host dynein adapter proteins such as dynein light chain 1 (DYNLL1), dynein light chain Tctex-type 1 (DYNLT1), and p150(Glued) in early steps of human immunodeficiency virus type 1 (HIV-1) replication...
  4. Meiri D, Marshall C, Mokady D, LaRose J, Mullin M, Gingras A, et al. Mechanistic insight into GPCR-mediated activation of the microtubule-associated RhoA exchange factor GEF-H1. Nat Commun. 2014;5:4857 pubmed publisher
    ..The coordinated displacement of GEF-H1 from microtubules by G-proteins and its dephosphorylation by PP2A demonstrate a multistep GEF-H1 activation and present a unique mechanism coupling GPCR signalling to Rho activation. ..
  5. Shi W, Xie Y, He J, Zhou J, Gao Y, Wei W, et al. Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia. Sci Rep. 2017;7:1866 pubmed publisher
    ..cilia was prevented by treating ROBs with cytochalasin D, but not with LiCl or dynein light chain Tctex-type 1 (Dynlt1) siRNA...
  6. Nagler M, Palkowitsch L, Rading S, Moepps B, Karsak M. Cannabinoid receptor 2 expression modulates Gβ(1)γ(2) protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1. Biochem Pharmacol. 2016;99:60-72 pubmed publisher
    ..These findings implicate that CB2 receptor expression modifies the stability of intracellular protein complexes by a non-canonical pathway. ..
  7. Ochiai K, Morimatsu M, Kato Y, Ishiguro Oonuma T, Udagawa C, Rungsuriyawiboon O, et al. Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity. Oncotarget. 2016;7:3283-96 pubmed publisher
  8. Cervantes Anaya N, Ponciano Gómez A, López Álvarez G, Gonzalez Reyes C, Hernández García S, Cabañas Cortés M, et al. Downregulation of sorting nexin 10 is associated with overexpression of miR-30d during liver cancer progression in rats. Tumour Biol. 2017;39:1010428317695932 pubmed publisher
    ..Based on a microarray analysis performed in our laboratory, we selected six genes-namely, ANXA2, DYNLT1, PFKP, PLA2G7, KRT19, and SNX10-as candidates for validation as tumor markers of liver cancer in a rat model...
  9. Li G, Huang J, Yang J, He D, Wang C, Qi X, et al. Structure based function-annotation of hypothetical protein MGG_01005 from Magnaporthe oryzae reveals it is the dynein light chain orthologue of dynlt1/3. Sci Rep. 2018;8:3952 pubmed publisher
    ..of the hypothetical protein, MGG_01005 and show that it is the Magnaporthe oryzae Dynein light chain Tctex-type 1 (dynlt1/3), demonstrated by its structural similarity to other orthologous dynlt1 proteins and its conserved interaction ..
  10. Houy S, Groffen A, Ziomkiewicz I, Verhage M, Pinheiro P, Sørensen J. Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs. elife. 2017;6: pubmed publisher
    ..We conclude that Doc2B acts as a vesicle priming protein. ..
  11. Lo K, Kogoy J, Rasoul B, King S, Pfister K. Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex. J Biol Chem. 2007;282:36871-8 pubmed
    ..Thus, whereas all combinations of DYNLT light chain dimers can be made, not all of the possible combinations of the isoforms are utilized during the assembly of the dynein complex. ..
  12. Caly L, Kassouf V, Moseley G, Diefenbach R, Cunningham A, Jans D. Fast track, dynein-dependent nuclear targeting of human immunodeficiency virus Vpr protein; impaired trafficking in a clinical isolate. Biochem Biophys Res Commun. 2016;470:735-740 pubmed publisher
    ..after photobleaching and other approaches to document interaction between Vpr and the dynein light chain protein, DYNLT1, and impaired interaction of the F72L mutant with DYNLT1...
  13. Gamerdinger M, Kaya A, Wolfrum U, Clement A, Behl C. BAG3 mediates chaperone-based aggresome-targeting and selective autophagy of misfolded proteins. EMBO Rep. 2011;12:149-56 pubmed publisher
    ..Notably, aggresome-targeting by BAG3 is distinct from previously described mechanisms, as it does not depend on substrate ubiquitination...
  14. Strovel E, Wu D, Sussman D. Protein phosphatase 2Calpha dephosphorylates axin and activates LEF-1-dependent transcription. J Biol Chem. 2000;275:2399-403 pubmed
    ..PP2C utilizes Axin as a substrate both in vitro and in vivo and decreases its half-life. These results indicate that PP2C is a positive regulator of Wnt signal transduction and mediates its effects through the dephosphorylation of Axin. ..
  15. Yeh C, Li A, Chuang J, Saito M, Caceres A, Sung C. IGF-1 activates a cilium-localized noncanonical G?? signaling pathway that regulates cell-cycle progression. Dev Cell. 2013;26:358-68 pubmed publisher
    ..These data suggest that during corticogenesis, a cilium-transduced, noncanonical IGF-1R-G??-phospho(T94)Tctex-1 signaling pathway promotes the proliferation of neural progenitors through modulation of ciliary resorption and G1 length. ..
  16. Fang Y, Xu X, Dang Y, Zhang Y, Zhang J, Hu J, et al. MAP4 mechanism that stabilizes mitochondrial permeability transition in hypoxia: microtubule enhancement and DYNLT1 interaction with VDAC1. PLoS ONE. 2011;6:e28052 pubmed publisher
    ..a yeast two-hybrid system to tag a protein interacting with mitochondria, dynein light chain Tctex-type 1 (DYNLT1), by hVDAC1 bait...
  17. Merino Gracia J, Zamora Carreras H, Bruix M, Rodríguez Crespo I. Molecular Basis for the Protein Recognition Specificity of the Dynein Light Chain DYNLT1/Tctex1: CHARACTERIZATION OF THE INTERACTION WITH ACTIVIN RECEPTOR IIB. J Biol Chem. 2016;291:20962-20975 pubmed
    It has been suggested that DYNLT1, a dynein light chain known to bind to various cellular and viral proteins, can function both as a molecular clamp and as a microtubule-cargo adapter...
  18. Watanabe T, Fujiwara T, Shimizu F, Okuno S, Suzuki M, Takahashi E, et al. Cloning, expression, and mapping of TCTEL1, a putative human homologue of murine Tcte1, to 6q. Cytogenet Cell Genet. 1996;73:153-6 pubmed
    ..The cDNA, designated TCTEL1, contained an open reading frame of 339 nucleotides encoding 113 amino acids...
  19. Duguay D, Bélanger Nelson E, Mongrain V, Beben A, Khatchadourian A, Cermakian N. Dynein light chain Tctex-type 1 modulates orexin signaling through its interaction with orexin 1 receptor. PLoS ONE. 2011;6:e26430 pubmed publisher
    ..experiments, we found interactions between OX1R and the dynein light chains Tctex-type 1 and 3 (Dynlt1, Dynlt3)...
  20. Brault J, Kudelko M, Vidalain P, Tangy F, Despr s P, Pardigon N. The interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication. Virology. 2011;417:369-78 pubmed publisher
    ..Silencing Tctex-1 inhibited the production of recombinant dengue subviral particles (RSPs). Thus Tctex-1 may play a role in late stages of viral replication through its interaction with the membrane protein...
  21. Tete R, de Montgolfier R, Piante M. [Leiomyoma and leiomyosarcoma of the small intestine. Value of selective angiography]. J Med Lyon. 1970;52:1523-6 passim pubmed
  22. Diefenbach R, Diefenbach E, Douglas M, Cunningham A. The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23. Biochemistry. 2002;41:14906-15 pubmed
    ..In support of separate binding sites on heavy chain for light chain and SNAPs, a complex of heavy and light chains was observed to interact with SNAP25 and SNAP23. ..
  23. Ohka S, Matsuda N, Tohyama K, Oda T, Morikawa M, Kuge S, et al. Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome. J Virol. 2004;78:7186-98 pubmed
    ..These results suggest that direct efficient interaction between the cytoplasmic domain and Tctex-1 is essential for the efficient retrograde transport of PV-containing vesicles along microtubules in vivo. ..
  24. Apcarian A, Cunningham A, Diefenbach R. Identification of binding domains in the herpes simplex virus type 1 small capsid protein pUL35 (VP26). J Gen Virol. 2010;91:2659-63 pubmed publisher
    ..Analysis of the binding of dynein light chain subunits, DYNLT1 and DYNLT3, as well the HSV-1 structural proteins pUL19 (VP5) and pUL37 was then undertaken using the LexA yeast ..
  25. Pavlos N, Cheng T, Qin A, Ng P, Feng H, Ang E, et al. Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption. Mol Cell Biol. 2011;31:1551-64 pubmed publisher
  26. Xu X, Zhang Q, Hu J, Zhang D, Jiang X, Jia J, et al. Phosphorylation of DYNLT1 at serine 82 regulates microtubule stability and mitochondrial permeabilization in hypoxia. Mol Cells. 2013;36:322-32 pubmed publisher
    ..Dynein light chain Tctex-type 1 (DYNLT1) is thought to be associated with MTs and mitochondria...
  27. Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, et al. Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1. Biochem Biophys Res Commun. 2011;412:391-5 pubmed publisher
    ..The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively. ..
  28. Mueller S, Cao X, Welker R, Wimmer E. Interaction of the poliovirus receptor CD155 with the dynein light chain Tctex-1 and its implication for poliovirus pathogenesis. J Biol Chem. 2002;277:7897-904 pubmed
    ..Association with Tctex-1 and, hence, with the dynein motor complex may offer an explanation for how poliovirus hijacks the cellular transport machinery to retrogradely ascend along the axon to the neuronal cell body. ..
  29. Baciu C, Thompson K, Mougeot J, Brooks B, Weller J. The LO-BaFL method and ALS microarray expression analysis. BMC Bioinformatics. 2012;13:244 pubmed publisher
    ..Control and six sALS samples yielded the predicted differential expression for 7 genes: TARDBP, SKIV2L2, C12orf35, DYNLT1, ACTG1, B2M, and ILKAP...
  30. Douglas M, Diefenbach R, Homa F, Miranda Saksena M, Rixon F, Vittone V, et al. Herpes simplex virus type 1 capsid protein VP26 interacts with dynein light chains RP3 and Tctex1 and plays a role in retrograde cellular transport. J Biol Chem. 2004;279:28522-30 pubmed
    ..We propose that VP26 mediates binding of incoming herpes simplex virus capsids to cytoplasmic dynein during cellular infection, through interactions with dynein light chains. ..
  31. Sarma N, Yaseen N. Dynein Light Chain 1 (DYNLT1) Interacts with Normal and Oncogenic Nucleoporins. PLoS ONE. 2013;8:e67032 pubmed publisher
    ..We identified Dynein Light Chain 1 (DYNLT1), an integral 14 KDa protein subunit of the large microtubule-based cytoplasmic dynein complex, as an interaction ..
  32. Vlach J, Lipov J, Rumlova M, Veverka V, Lang J, Srb P, et al. D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein. Proc Natl Acad Sci U S A. 2008;105:10565-70 pubmed publisher
    ..This conformational change buries a putative intracellular targeting motif located between both domains in the hydrophobic pocket of the MA molecule, thereby preventing the interaction with cellular transport mechanisms...
  33. Indu S, Sekhar S, Sengottaiyan J, Kumar A, Pillai S, Laloraya M, et al. Aberrant Expression of Dynein light chain 1 (DYNLT1) is Associated with Human Male Factor Infertility. Mol Cell Proteomics. 2015;14:3185-95 pubmed publisher
    b>DYNLT1 is a member of a gene family identified within the t-complex of the mouse, which has been linked with male germ cell development and function in the mouse and the fly...
  34. Li A, Saito M, Chuang J, Tseng Y, Dedesma C, Tomizawa K, et al. Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors. Nat Cell Biol. 2011;13:402-11 pubmed publisher
  35. Merino Gracia J, García Mayoral M, Rapali P, Valero R, Bruix M, Rodríguez Crespo I. DYNLT (Tctex-1) forms a tripartite complex with dynein intermediate chain and RagA, hence linking this small GTPase to the dynein motor. FEBS J. 2015;282:3945-58 pubmed publisher
    ..In summary, our results shed light on the mechanisms adopted by DYNLT when binding to protein cargoes that become transported alongside microtubules bound to the dynein motor. ..
  36. Mital J, Lutter E, Barger A, Dooley C, Hackstadt T. Chlamydia trachomatis inclusion membrane protein CT850 interacts with the dynein light chain DYNLT1 (Tctex1). Biochem Biophys Res Commun. 2015;462:165-70 pubmed publisher
    ..membrane protein CT850 against a HeLa cell cDNA library and identified an interaction with the dynein light chain DYNLT1 (Tctex1)...
  37. Takizawa N, Smith T, Nebl T, Crowley J, Palmieri S, Lifshitz L, et al. Supervillin modulation of focal adhesions involving TRIP6/ZRP-1. J Cell Biol. 2006;174:447-58 pubmed
    ..Thus, SV interactions with TRIP6 at FAs promote loss of FA structure and function. SV and TRIP6 binding partners suggest several specific mechanisms through which the SV-TRIP6 interaction may regulate FA maturation and/or disassembly. ..
  38. de Wit N, Verschuure P, Kappé G, King S, de Jong W, Van Muijen G, et al. Testis-specific human small heat shock protein HSPB9 is a cancer/testis antigen, and potentially interacts with the dynein subunit TCTEL1. Eur J Cell Biol. 2004;83:337-45 pubmed
    ..b>TCTEL1, a light chain component of cytoplasmic and flagellar dynein, interacted in both the yeast two-hybrid system and ..
  39. Saito M, Otsu W, Hsu K, Chuang J, Yanagisawa T, Shieh V, et al. Tctex-1 controls ciliary resorption by regulating branched actin polymerization and endocytosis. EMBO Rep. 2017;18:1460-1472 pubmed publisher
  40. Midoux P, Pigeon L, Gonçalves C, Pichon C. Peptides mediating DNA transport on microtubules and their impact on non-viral gene transfer efficiency. Biosci Rep. 2017;37: pubmed publisher
    ..interaction feature is presented followed by a summary of the results obtained by exploitation of LC8 and TCTEL1 dynein light chain association sequence (DLC-AS) for non-viral transfection...
  41. Sandi M, Marshall C, Balan M, Coyaud E, Zhou M, Monson D, et al. MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity. Sci Signal. 2017;10: pubmed publisher
    ..microtubule network and sequestered in an inhibited state through binding to dynein light chain Tctex-1 type 1 (DYNLT1)...
  42. Nekrasova O, Harmon R, Broussard J, Koetsier J, Godsel L, Fitz G, et al. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat Commun. 2018;9:1053 pubmed publisher
    ..Moreover, Dsg1 is sufficient to enable simple epithelial cells to exit a monolayer to form a second layer, highlighting its morphogenetic potential. ..
  43. Schneider M, Spoden G, Florin L, Lambert C. Identification of the dynein light chains required for human papillomavirus infection. Cell Microbiol. 2011;13:32-46 pubmed publisher
    ..We have performed yeast two-hybrid screening and identified the dynein light chain DYNLT1 (previously called Tctex1) as interaction partner of HPV16 L2...
  44. Asante D, Stevenson N, Stephens D. Subunit composition of the human cytoplasmic dynein-2 complex. J Cell Sci. 2014;127:4774-87 pubmed publisher
    ..We define several subunits common to both dynein-1 and dynein-2, including TCTEX-1 (also known as DYNLT1) and TCTEX-3 (also known as DYNLT3), roadblock-1 (also known as DYNLRB1) and roadblock-2 (also known as DYNLRB2), ..
  45. Yeh T, Peretti D, Chuang J, Rodriguez Boulan E, Sung C. Regulatory dissociation of Tctex-1 light chain from dynein complex is essential for the apical delivery of rhodopsin. Traffic. 2006;7:1495-502 pubmed
    ..Taken together, these results suggest that the dynein complex disassembles to release cargo due to the specific phosphorylation of Tctex-1 at the S82 residue and that this process is critical for the apical delivery of membrane cargoes. ..
  46. Liu C, Chuang J, Sung C, Mao Y. A dynein independent role of Tctex-1 at the kinetochore. Cell Cycle. 2015;14:1379-88 pubmed publisher
    ..Thus, our findings reveal a dynein independent role of Tctex-1 at the kinetochore to enhance the stability of kinetochore-microtubule attachment. ..