DNM1L

Summary

Gene Symbol: DNM1L
Description: dynamin 1 like
Alias: DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5, dynamin-1-like protein, Dnm1p/Vps1p-like protein, dynamin family member proline-rich carboxyl-terminal domain less, dynamin-like protein 4, dynamin-like protein IV, dynamin-related protein 1
Species: human
Products:     DNM1L

Top Publications

  1. Palmer C, Osellame L, Laine D, Koutsopoulos O, Frazier A, Ryan M. MiD49 and MiD51, new components of the mitochondrial fission machinery. EMBO Rep. 2011;12:565-73 pubmed publisher
    ..MiD49/51 form foci and rings around mitochondria similar to the fission mediator dynamin-related protein 1 (Drp1)...
  2. Chang C, Manlandro C, Arnoult D, Stadler J, Posey A, Hill R, et al. A lethal de novo mutation in the middle domain of the dynamin-related GTPase Drp1 impairs higher order assembly and mitochondrial division. J Biol Chem. 2010;285:32494-503 pubmed publisher
    ..b>Drp1 is a dynamin-related GTPase required for mitochondrial fission in mammalian cells...
  3. Karbowski M, Neutzner A, Youle R. The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division. J Cell Biol. 2007;178:71-84 pubmed
    ..The aberrant mitochondrial phenotypes in MARCH5 RING mutant-expressing cells are reversed by ectopic expression of Drp1, but not another mitochondrial fission protein Fis1...
  4. Santel A, Frank S. Shaping mitochondria: The complex posttranslational regulation of the mitochondrial fission protein DRP1. IUBMB Life. 2008;60:448-55 pubmed publisher
    ..Here we specifically focus on recent advances in our understanding of the regulation, activity, and function of dynamin-related protein 1, the main factor for controlled mitochondrial fission. ..
  5. Nakamura N, Kimura Y, Tokuda M, Honda S, Hirose S. MARCH-V is a novel mitofusin 2- and Drp1-binding protein able to change mitochondrial morphology. EMBO Rep. 2006;7:1019-22 pubmed
    Mitofusins and Drp1 are key components in mitochondrial membrane fusion and division, but the molecular mechanism underlying the regulation of their activities remains to be clarified...
  6. Palmer C, Elgass K, Parton R, Osellame L, Stojanovski D, Ryan M. Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission. J Biol Chem. 2013;288:27584-93 pubmed publisher
    b>Drp1 (dynamin-related protein 1) is recruited to both mitochondrial and peroxisomal membranes to execute fission. Fis1 and Mff are Drp1 receptor/effector proteins of mitochondria and peroxisomes...
  7. Zhao J, Liu T, Jin S, Wang X, Qu M, Uhlen P, et al. Human MIEF1 recruits Drp1 to mitochondrial outer membranes and promotes mitochondrial fusion rather than fission. EMBO J. 2011;30:2762-78 pubmed publisher
    Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Drp1 (dynamin-related protein 1) and hFis1 are two key players of mitochondrial fission, but how Drp1 is recruited to mitochondria and how Drp1-mediated ..
  8. Möpert K, Hajek P, Frank S, Chen C, Kaufmann J, Santel A. Loss of Drp1 function alters OPA1 processing and changes mitochondrial membrane organization. Exp Cell Res. 2009;315:2165-80 pubmed publisher
    RNAi mediated loss of Drp1 function changes mitochondrial morphology in cultured HeLa and HUVEC cells by shifting the balance of mitochondrial fission and fusion towards unopposed fusion...
  9. Shin H, Shinotsuka C, Torii S, Murakami K, Nakayama K. Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p. J Biochem. 1997;122:525-30 pubmed
    ..In this study, we identified a novel human protein, named Dnm1p/Vps1p-like protein (DVLP)...

More Information

Publications68

  1. Korobova F, Ramabhadran V, Higgs H. An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2. Science. 2013;339:464-7 pubmed publisher
    Mitochondrial fission is fundamentally important to cellular physiology. The dynamin-related protein Drp1 mediates fission, and interaction between mitochondrion and endoplasmic reticulum (ER) enhances fission...
  2. Cho D, Nakamura T, Fang J, Cieplak P, Godzik A, Gu Z, et al. S-nitrosylation of Drp1 mediates beta-amyloid-related mitochondrial fission and neuronal injury. Science. 2009;324:102-5 pubmed publisher
    ..fission, synaptic loss, and neuronal damage, in part via S-nitrosylation of dynamin-related protein 1 (forming SNO-Drp1). Preventing nitrosylation of Drp1 by cysteine mutation abrogated these neurotoxic events...
  3. Koch A, Thiemann M, Grabenbauer M, Yoon Y, McNiven M, Schrader M. Dynamin-like protein 1 is involved in peroxisomal fission. J Biol Chem. 2003;278:8597-605 pubmed
    The mammalian dynamin-like protein 1 (DLP1), a member of the dynamin family of large GTPases, possesses mechanochemical properties known to constrict and tubulate membranes...
  4. Song W, Chen J, Petrilli A, Liot G, Klinglmayr E, Zhou Y, et al. Mutant huntingtin binds the mitochondrial fission GTPase dynamin-related protein-1 and increases its enzymatic activity. Nat Med. 2011;17:377-82 pubmed publisher
    ..Mutant huntingtin abnormally interacts with the mitochondrial fission GTPase dynamin-related protein-1 (DRP1) in mice and humans with Huntington's disease, which, in turn, stimulates its enzymatic activity...
  5. Han X, Lu Y, Li S, Kaitsuka T, Sato Y, Tomizawa K, et al. CaM kinase I alpha-induced phosphorylation of Drp1 regulates mitochondrial morphology. J Cell Biol. 2008;182:573-85 pubmed publisher
    ..VDCC-associated Ca2+ signaling stimulates phosphorylation of dynamin-related protein 1 (Drp1) at serine 600 via activation of Ca2+/calmodulin-dependent protein kinase Ialpha (CaMKIalpha)...
  6. Imoto M, Tachibana I, Urrutia R. Identification and functional characterization of a novel human protein highly related to the yeast dynamin-like GTPase Vps1p. J Cell Sci. 1998;111 ( Pt 10):1341-9 pubmed
    ..study, we report the identification and characterization of a novel gene encoding a human dynamin-related protein, DRP1, displaying high similarity to the Golgi dynamin-like protein Vps1p from yeast and to a Caenorhabditis elegans ..
  7. Wang S, Song J, Tan M, Albers K, Jia J. Mitochondrial fission proteins in peripheral blood lymphocytes are potential biomarkers for Alzheimer's disease. Eur J Neurol. 2012;19:1015-22 pubmed publisher
    Expression of the mitochondrial fission proteins dynamin-related protein 1 (Drp1), S-nitrosylated Drp1 (SNO-Drp1), and Fis1 has been found to be altered in brain tissues and skin fibroblasts from patients with Alzheimer's disease (AD)...
  8. Gandre Babbe S, van der Bliek A. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Mol Biol Cell. 2008;19:2402-12 pubmed publisher
    ..to the mitochondrial networks formed when cells are transfected with siRNA for two established fission proteins, Drp1 and Fis1...
  9. Kamimoto T, Nagai Y, Onogi H, Muro Y, Wakabayashi T, Hagiwara M. Dymple, a novel dynamin-like high molecular weight GTPase lacking a proline-rich carboxyl-terminal domain in mammalian cells. J Biol Chem. 1998;273:1044-51 pubmed
    We have cloned human dymple, a novel dynamin family member. The full-length cDNA sequence encodes a protein composed of 736 amino acids with a molecular mass of 80 kDa. This amino acid sequence most resembles yeast DNM1P and VPS1P...
  10. Chang C, Blackstone C. Dynamic regulation of mitochondrial fission through modification of the dynamin-related protein Drp1. Ann N Y Acad Sci. 2010;1201:34-9 pubmed publisher
    ..The mammalian Drp1 protein plays a key role in fission, while Mfn1, Mfn2, and OPA1 are required for fusion...
  11. Cribbs J, Strack S. Reversible phosphorylation of Drp1 by cyclic AMP-dependent protein kinase and calcineurin regulates mitochondrial fission and cell death. EMBO Rep. 2007;8:939-44 pubmed
    ..Dynamin-related protein 1 (Drp1) is an ancient mechanoenzyme that uses GTP hydrolysis to power the constriction and division of mitochondria...
  12. Reddy P, Reddy T, Manczak M, Calkins M, Shirendeb U, Mao P. Dynamin-related protein 1 and mitochondrial fragmentation in neurodegenerative diseases. Brain Res Rev. 2011;67:103-18 pubmed publisher
    ..The GTPase family of proteins, including fission proteins, dynamin related protein 1 (Drp1), mitochondrial fission 1 (Fis1), and fusion proteins (Mfn1, Mfn2 and Opa1) are essential to ..
  13. Waterham H, Koster J, van Roermund C, Mooyer P, Wanders R, Leonard J. A lethal defect of mitochondrial and peroxisomal fission. N Engl J Med. 2007;356:1736-41 pubmed
    ..and peroxisomes, as well as a heterozygous, dominant-negative mutation in the dynamin-like protein 1 gene (DLP1). The DLP1 protein has previously been implicated, in vitro, in the fission of both these organelles...
  14. Taguchi N, Ishihara N, Jofuku A, Oka T, Mihara K. Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission. J Biol Chem. 2007;282:11521-9 pubmed
    ..are dynamic entities, frequently dividing and fusing with each other, during which dynamin-related GTPase Drp1 is required for the fission reaction. In this study, we analyzed mitochondrial dynamics in mitotic mammalian cells...
  15. Wang H, Song P, Du L, Tian W, Yue W, Liu M, et al. Parkin ubiquitinates Drp1 for proteasome-dependent degradation: implication of dysregulated mitochondrial dynamics in Parkinson disease. J Biol Chem. 2011;286:11649-58 pubmed publisher
    ..Here, we demonstrate that Parkin interacts with and subsequently ubiquitinates dynamin-related protein 1 (Drp1), for promoting its proteasome-dependent degradation...
  16. Wang X, Su B, Liu W, He X, Gao Y, Castellani R, et al. DLP1-dependent mitochondrial fragmentation mediates 1-methyl-4-phenylpyridinium toxicity in neurons: implications for Parkinson's disease. Aging Cell. 2011;10:807-23 pubmed publisher
    ..fragmentation followed by a second wave of increase in mitochondrial fragmentation, along with increased DLP1 expression and mitochondrial translocation...
  17. Zhang B, Davidson M, Zhou H, Wang C, Walker W, Hei T. Cytoplasmic irradiation results in mitochondrial dysfunction and DRP1-dependent mitochondrial fission. Cancer Res. 2013;73:6700-10 pubmed publisher
    ..mitochondrial fragmentation or fission was associated with increased expression of the dynamin-like protein DRP1, which promotes mitochondrial fission...
  18. Koirala S, Guo Q, Kalia R, Bui H, Eckert D, Frost A, et al. Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission. Proc Natl Acad Sci U S A. 2013;110:E1342-51 pubmed publisher
    Mitochondrial fission is mediated by the dynamin-related GTPases Dnm1/Drp1 (yeast/mammals), which form spirals around constricted sites on mitochondria...
  19. Karbowski M, Lee Y, Gaume B, Jeong S, Frank S, Nechushtan A, et al. Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis. J Cell Biol. 2002;159:931-8 pubmed
    ..A dominant negative mutant of Drp1, Drp1K38A, inhibits apoptotic scission of mitochondria, but does not inhibit Bax translocation or coalescence into ..
  20. Hong Y, Chen C, Cheng D, Howng S, Chow C. Human dynamin-like protein interacts with the glycogen synthase kinase 3beta. Biochem Biophys Res Commun. 1998;249:697-703 pubmed
    ..3 beta (Gsk-3 beta) as bait in the yeast two-hybrid system, we identified a novel human dynamin-like protein IV (HdynIV)...
  21. Sandebring A, Thomas K, Beilina A, Van Der Brug M, Cleland M, Ahmad R, et al. Mitochondrial alterations in PINK1 deficient cells are influenced by calcineurin-dependent dephosphorylation of dynamin-related protein 1. PLoS ONE. 2009;4:e5701 pubmed publisher
    ..Expression of dynamin-related protein 1 (Drp1) exaggerates PINK1 deficiency phenotypes and Drp1 RNAi rescues them...
  22. Chiang Y, Chen S, Hsiao Y, Huang C, Lin T, Chiang I, et al. Nuclear expression of dynamin-related protein 1 in lung adenocarcinomas. Mod Pathol. 2009;22:1139-50 pubmed publisher
    Dynamin-related protein 1 (DRP1), an 80 kDa GTPase, is involved in mitochondrial fission and anticancer drug-mediated cytotoxicity, which implicate an association with disease progression of cancer...
  23. Figueroa Romero C, INIGUEZ LLUHI J, Stadler J, Chang C, Arnoult D, Keller P, et al. SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle. FASEB J. 2009;23:3917-27 pubmed publisher
    ..b>Drp1 associates with mitochondrial fission sites and promotes membrane constriction through its intrinsic GTPase ..
  24. Fröhlich C, Grabiger S, Schwefel D, Faelber K, Rosenbaum E, Mears J, et al. Structural insights into oligomerization and mitochondrial remodelling of dynamin 1-like protein. EMBO J. 2013;32:1280-92 pubmed publisher
    Dynamin 1-like protein (DNM1L) mediates fission of mitochondria and peroxisomes, and dysfunction of DNM1L has been implicated in several neurological disorders...
  25. Montessuit S, Somasekharan S, Terrones O, Lucken Ardjomande S, Herzig S, Schwarzenbacher R, et al. Membrane remodeling induced by the dynamin-related protein Drp1 stimulates Bax oligomerization. Cell. 2010;142:889-901 pubmed publisher
    ..These events are accompanied by mitochondrial fission that appears to require Drp1, a large GTPase of the dynamin superfamily...
  26. Losón O, Song Z, Chen H, Chan D. Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission. Mol Biol Cell. 2013;24:659-67 pubmed publisher
    ..been proposed to promote mitochondrial fission by recruiting the GTPase dynamin-related protein 1 (Drp1), but fundamental issues remain concerning their function...
  27. Braschi E, Zunino R, McBride H. MAPL is a new mitochondrial SUMO E3 ligase that regulates mitochondrial fission. EMBO Rep. 2009;10:748-54 pubmed publisher
    ..SUMOylation of the mitochondrial fission GTPase dynamin-related protein 1 (DRP1) stimulates mitochondrial fission, suggesting that SUMOylation has an important function in mitochondrial dynamics...
  28. Chang C, Blackstone C. Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology. J Biol Chem. 2007;282:21583-7 pubmed
    ..The multimeric dynamin-like GTPase Drp1 is a critical protein mediating mitochondrial division...
  29. Lee Y, Jeong S, Karbowski M, Smith C, Youle R. Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis. Mol Biol Cell. 2004;15:5001-11 pubmed
    ..Using short hairpin RNAs for RNA interference, we manipulated the expression levels of the proteins hFis1, Drp1, and Opa1 that are involved in mitochondrial fission and fusion in mammalian cells, and we characterized their ..
  30. Yoon Y, Krueger E, Oswald B, McNiven M. The mitochondrial protein hFis1 regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1. Mol Cell Biol. 2003;23:5409-20 pubmed
    ..In mammalian cells, the dynamin-like protein DLP1/Drp1 functions as a mitochondrial fission protein, but the mechanisms by which DLP1/Drp1 and the mitochondrial membrane ..
  31. Wenger J, Klinglmayr E, Fröhlich C, Eibl C, Gimeno A, Hessenberger M, et al. Functional mapping of human dynamin-1-like GTPase domain based on x-ray structure analyses. PLoS ONE. 2013;8:e71835 pubmed publisher
    Human dynamin-1-like protein (DNM1L) is a GTP-driven molecular machine that segregates mitochondria and peroxisomes...
  32. Yoon Y, Pitts K, Dahan S, McNiven M. A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells. J Cell Biol. 1998;140:779-93 pubmed
    ..biological methods were used to isolate a cDNA clone encoding an 80-kilodalton novel dynamin-like protein, DLP1, that shares up to 42% homology with other dynamin-related proteins...
  33. Dagda R, Cherra S, Kulich S, Tandon A, Park D, Chu C. Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission. J Biol Chem. 2009;284:13843-55 pubmed publisher
    ..The dominant negative Drp1 mutant inhibited both fission and mitophagy in PINK1-deficient cells...
  34. Cereghetti G, Stangherlin A, Martins de Brito O, Chang C, Blackstone C, Bernardi P, et al. Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria. Proc Natl Acad Sci U S A. 2008;105:15803-8 pubmed publisher
    ..Here, we show a role for calcineurin-dependent translocation of the profission dynamin related protein 1 (Drp1) to mitochondria in dysfunction-induced fragmentation...
  35. Estaquier J, Arnoult D. Inhibiting Drp1-mediated mitochondrial fission selectively prevents the release of cytochrome c during apoptosis. Cell Death Differ. 2007;14:1086-94 pubmed
    ..Dynamin-related protein 1 (Drp1), a protein of the mitochondrial fission machinery, has been reported to participate in apoptotic mitochondrial ..
  36. Yonashiro R, Ishido S, Kyo S, Fukuda T, Goto E, Matsuki Y, et al. A novel mitochondrial ubiquitin ligase plays a critical role in mitochondrial dynamics. EMBO J. 2006;25:3618-26 pubmed
    ..Indeed, a dominant-negative expression of Drp1 mutant blocked mitochondrial fragmentation induced by MITOL depletion...
  37. Breckenridge D, Stojanovic M, Marcellus R, Shore G. Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol. J Cell Biol. 2003;160:1115-27 pubmed
    ..early release of Ca2+ from the ER, concomitant uptake of Ca2+ into mitochondria, and mitochondrial recruitment of Drp1, a dynamin-related protein that mediates scission of the outer mitochondrial membrane, resulting in dramatic ..
  38. Smirnova E, Griparic L, Shurland D, van der Bliek A. Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells. Mol Biol Cell. 2001;12:2245-56 pubmed
    Mutations in the human dynamin-related protein Drp1 cause mitochondria to form perinuclear clusters. We show here that these mitochondrial clusters consist of highly interconnected mitochondrial tubules...
  39. Wang X, Yan M, Fujioka H, Liu J, Wilson Delfosse A, Chen S, et al. LRRK2 regulates mitochondrial dynamics and function through direct interaction with DLP1. Hum Mol Genet. 2012;21:1931-44 pubmed publisher
    ..WT) LRRK2 expression caused mitochondrial fragmentation along with increased mitochondrial dynamin-like protein (DLP1, also known as DRP1), a fission protein, which was further exacerbated by expression of PD-associated mutants (..
  40. Niu J, Yu M, Wang C, Xu Z. Leucine-rich repeat kinase 2 disturbs mitochondrial dynamics via Dynamin-like protein. J Neurochem. 2012;122:650-8 pubmed publisher
    ..In neurons, endogenous LRRK2 and the mitochondrial fission factor Dynamin like protein 1 (DLP1) interact with and partially co-localize with each other...
  41. Lin H, Lai R, Lin S, Lin R, Wang M, Lin C, et al. Suppressor of cytokine signaling 6 (SOCS6) promotes mitochondrial fission via regulating DRP1 translocation. Cell Death Differ. 2013;20:139-53 pubmed publisher
    ..SOCS6 is targeted to mitochondria and induces mitochondrial fragmentation mediated through an increase in DRP1 fission activity...
  42. De Palma C, Falcone S, Pisoni S, Cipolat S, Panzeri C, Pambianco S, et al. Nitric oxide inhibition of Drp1-mediated mitochondrial fission is critical for myogenic differentiation. Cell Death Differ. 2010;17:1684-96 pubmed publisher
    ..is due to the enhanced activity, translocation and docking of the pro-fission GTPase dynamin-related protein-1 (Drp1) to mitochondria, leading also to a latent mitochondrial dysfunction that increased sensitivity to apoptotic ..
  43. Otera H, Wang C, Cleland M, Setoguchi K, Yokota S, Youle R, et al. Mff is an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells. J Cell Biol. 2010;191:1141-58 pubmed publisher
    The cytoplasmic dynamin-related guanosine triphosphatase Drp1 is recruited to mitochondria and mediates mitochondrial fission...
  44. Park Y, Lee S, Karbowski M, Neutzner A, Youle R, Cho H. Loss of MARCH5 mitochondrial E3 ubiquitin ligase induces cellular senescence through dynamin-related protein 1 and mitofusin 1. J Cell Sci. 2010;123:619-26 pubmed publisher
    ..has been identified as a molecule that binds mitochondrial fission 1 protein (hFis1), dynamin-related protein 1 (Drp1) and mitofusin 2 (Mfn2), key proteins in the control of mitochondrial fission and fusion...
  45. Pitts K, McNiven M, Yoon Y. Mitochondria-specific function of the dynamin family protein DLP1 is mediated by its C-terminal domains. J Biol Chem. 2004;279:50286-94 pubmed
    ..One of these family members, DLP1/Drp1, is similar to conventional dynamins as it contains an N-terminal GTPase domain followed by a middle region (MID), ..
  46. Smirnova E, Shurland D, Ryazantsev S, van der Bliek A. A human dynamin-related protein controls the distribution of mitochondria. J Cell Biol. 1998;143:351-8 pubmed
    ..We present evidence that a human dynamin-related protein (Drp1) is specifically required to establish this morphology...
  47. Wang Z, Jiang H, Chen S, Du F, Wang X. The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways. Cell. 2012;148:228-43 pubmed publisher
    ..Upon necrosis induction, PGAM5S recruited the mitochondrial fission factor Drp1 and activated its GTPase activity by dephosphorylating the serine 637 site of Drp1...
  48. Rasmussen R, Rusak J, Price G, Robinson P, Simpson R, Dorow D. Mixed-lineage kinase 2-SH3 domain binds dynamin and greatly enhances activation of GTPase by phospholipid. Biochem J. 1998;335 ( Pt 1):119-24 pubmed
    ..These results suggest that synergy between phospholipid and SH3 domain binding might be a general mechanism for the regulation of GTP hydrolysis by dynamin. ..
  49. Cherubini M, Puigdellívol M, Alberch J, Gines S. Cdk5-mediated mitochondrial fission: A key player in dopaminergic toxicity in Huntington's disease. Biochim Biophys Acta. 2015;1852:2145-60 pubmed publisher
    ..mitochondrial fission in mutant huntingtin striatal cells can be a consequence of Cdk5-mediated alterations in Drp1 subcellular distribution and activity since pharmacological or genetic inhibition of Cdk5 normalizes Drp1 function ..
  50. Hering T, Kojer K, Birth N, Hallitsch J, Taanman J, Orth M. Mitochondrial cristae remodelling is associated with disrupted OPA1 oligomerisation in the Huntington's disease R6/2 fragment model. Exp Neurol. 2017;288:167-175 pubmed publisher
    ..microscopy, and transcript and protein levels of factors that play a key role in fission and fusion, including DRP1, mitofusin 1 and 2, mitofilin and OPA1, and cytochrome c and caspase 3 were assessed by RT-qPCR and immunoblotting...
  51. Smaczynska de Rooij I, Marklew C, Allwood E, Palmer S, Booth W, Mishra R, et al. Phosphorylation Regulates the Endocytic Function of the Yeast Dynamin-Related Protein Vps1. Mol Cell Biol. 2015;36:742-55 pubmed publisher
    ..Our data support a model in which phosphorylation and dephosphorylation of Vps1 promote distinct interactions and highlight the importance of such regulatory events in facilitating sequential progression of the endocytic process. ..
  52. Li C, Wang D, Wu W, Yang W, Ali Shah S, Zhao Y, et al. DLP1-dependent mitochondrial fragmentation and redistribution mediate prion-associated mitochondrial dysfunction and neuronal death. Aging Cell. 2018;17: pubmed publisher
    ..Dynamin-like protein 1 (DLP1) is one of the key regulators of mitochondrial fission...
  53. Goud Gadila S, Williams M, Saimani U, Delgado Cruz M, Makaraci P, Woodman S, et al. Yeast dynamin Vps1 associates with clathrin to facilitate vesicular trafficking and controls Golgi homeostasis. Eur J Cell Biol. 2017;96:182-197 pubmed publisher
    ..In addition, we found that Vps1, without the aid of clathrin, plays a role in controlling the number and turnover of late Golgi. ..
  54. Córdova Dávalos L, Carrera Calvo D, Solís Navarrete J, Mercado Gómez O, Arriaga Ávila V, Agredano Moreno L, et al. Status epilepticus triggers early mitochondrial fusion in the rat hippocampus in a lithium-pilocarpine model. Epilepsy Res. 2016;123:11-9 pubmed publisher
    ..and 72h after SE, and the cytoplasmic and mitochondrial fractions of the cells were used to analyze changes in the Drp1 and Fis1 fission proteins and the Mfn1 and Opa1 fusion proteins by western blot analysis...
  55. Ramachandran R. Mitochondrial dynamics: The dynamin superfamily and execution by collusion. Semin Cell Dev Biol. 2018;76:201-212 pubmed publisher
    ..Long believed to be the singular handiwork of dynamin-related protein 1 (Drp1), a cytosolic family member that transiently localizes to the mitochondrial surface, the execution of mitochondrial ..
  56. Pagliuso A, Cossart P, Stavru F. The ever-growing complexity of the mitochondrial fission machinery. Cell Mol Life Sci. 2018;75:355-374 pubmed publisher
    ..In contrast, the soluble dynamin-related GTPase Drp1 is recruited from the cytosol to mitochondria and is key to mitochondrial fission...
  57. Yang Z, Feng Z, Gu J, Li X, Dong Q, Liu K, et al. microRNA-488 inhibits chemoresistance of ovarian cancer cells by targeting Six1 and mitochondrial function. Oncotarget. 2017;8:80981-80993 pubmed publisher
    ..that miR-488 regulated mitochondrial fission/fusion balance and inhibited mitochondrial membrane potential, with p-Drp1, Drp1 and Fis1 downregulation. Luciferase reporter assay showed that Six1 is a target of miR-488...
  58. Ban Ishihara R, Tomohiro Takamiya S, Tani M, Baudier J, Ishihara N, Kuge O. COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1. FEBS Lett. 2015;589:3126-32 pubmed publisher
    ..mitochondria were highly elongated in ccdc56-repressed cells by the defective recruitment of the fission factor Drp1. We also found that mild and chronic inhibition of COX led to mitochondrial elongation, as seen in ccdc56-repressed ..
  59. Tsuyoshi H, Wong V, Han Y, Orisaka M, Yoshida Y, Tsang B. Saikosaponin-d, a calcium mobilizing agent, sensitizes chemoresistant ovarian cancer cells to cisplatin-induced apoptosis by facilitating mitochondrial fission and G2/M arrest. Oncotarget. 2017;8:99825-99840 pubmed publisher
    ..is mediated via calcium signaling, up-regulation of the mitochondrial fission proteins Dynamin-related protein 1 (Drp1) and optic atrophy 1 (Opa1), and loss in mitochondrial membrane potential (MMP)...