DNA polymerase lambda


Gene Symbol: DNA polymerase lambda
Description: DNA polymerase lambda
Alias: BETAN, POLKAPPA, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, polymerase (DNA directed), lambda, polymerase (DNA) lambda
Species: human
Products:     DNA polymerase lambda

Top Publications

  1. Fiala K, Abdel Gawad W, Suo Z. Pre-steady-state kinetic studies of the fidelity and mechanism of polymerization catalyzed by truncated human DNA polymerase lambda. Biochemistry. 2004;43:6751-62 pubmed
    b>DNA polymerase lambda (Pollambda), a member of the X-family DNA polymerases, possesses an N-terminal BRCT domain, a proline-rich domain, and a C-terminal polymerase beta-like domain (tPollambda)...
  2. Garcia Diaz M, Dominguez O, López Fernández L, de Lera L, Saníger M, Ruiz J, et al. DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA polymerase with a potential role in meiosis. J Mol Biol. 2000;301:851-67 pubmed
    ..These first evidences, although indirect, suggest a potential role of Pol lambda in DNA repair synthesis associated with meiosis. ..
  3. Braithwaite E, Kedar P, Stumpo D, Bertocci B, Freedman J, Samson L, et al. DNA polymerases beta and lambda mediate overlapping and independent roles in base excision repair in mouse embryonic fibroblasts. PLoS ONE. 2010;5:e12229 pubmed publisher
    ..One such polymerase, DNA polymerase lambda (pol lambda), was shown to be important in BER of oxidative DNA damage...
  4. Derose E, Kirby T, Mueller G, Bebenek K, Garcia Diaz M, Blanco L, et al. Solution structure of the lyase domain of human DNA polymerase lambda. Biochemistry. 2003;42:9564-74 pubmed
    b>DNA polymerase lambda (pol lambda) is a recently discovered nuclear enzyme belonging to the pol X family of DNA polymerases that exhibits a 32% sequence identity with the nuclear DNA repair protein, pol beta...
  5. Maga G, Crespan E, Wimmer U, van Loon B, Amoroso A, Mondello C, et al. Replication protein A and proliferating cell nuclear antigen coordinate DNA polymerase selection in 8-oxo-guanine repair. Proc Natl Acad Sci U S A. 2008;105:20689-94 pubmed publisher
  6. Maga G, Villani G, Ramadan K, Shevelev I, Tanguy Le Gac N, Blanco L, et al. Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis. J Biol Chem. 2002;277:48434-40 pubmed
    ..Our results suggest that the complex between PCNA and pol lambda may play an important role in the bypass of abasic sites in human cells. ..
  7. Maga G, Ramadan K, Locatelli G, Shevelev I, Spadari S, Hubscher U. DNA elongation by the human DNA polymerase lambda polymerase and terminal transferase activities are differentially coordinated by proliferating cell nuclear antigen and replication protein A. J Biol Chem. 2005;280:1971-81 pubmed
    b>DNA polymerase lambda contains template-dependent (DNA polymerase) and template-independent (terminal transferase) activities...
  8. Picher A, Blanco L. Human DNA polymerase lambda is a proficient extender of primer ends paired to 7,8-dihydro-8-oxoguanine. DNA Repair (Amst). 2007;6:1749-56 pubmed
  9. Kelley J, Turkheimer K, Haney M, Swanson W. Targeted resequencing of two genes, RAGE and POLL, confirms findings from a genome-wide scan for adaptive evolution and provides evidence for positive selection in additional populations. Hum Mol Genet. 2009;18:779-84 pubmed publisher
    ..In addition to confirming the signatures of selection from the genome-wide scan, direct resequencing reveals more extensive patterns of selection than the genotype data. ..

More Information


  1. Cisneros G, Perera L, Garcia Diaz M, Bebenek K, Kunkel T, Pedersen L. Catalytic mechanism of human DNA polymerase lambda with Mg2+ and Mn2+ from ab initio quantum mechanical/molecular mechanical studies. DNA Repair (Amst). 2008;7:1824-34 pubmed publisher
    ..Recently, the crystal structures of human DNA polymerase lambda (Pollambda) have been reported both for pre- and post-catalytic complexes [García-Díaz et al...
  2. Shevelev I, Blanca G, Villani G, Ramadan K, Spadari S, Hubscher U, et al. Mutagenesis of human DNA polymerase lambda: essential roles of Tyr505 and Phe506 for both DNA polymerase and terminal transferase activities. Nucleic Acids Res. 2003;31:6916-25 pubmed
    ..Our results suggest the existence of a common nucleotide-binding site for the polymerase and terminal transferase activities of pol lambda, as well as distinct roles of the amino acids Tyr505 and Phe506 in these two catalytic functions. ..
  3. Maga G, Villani G, Crespan E, Wimmer U, Ferrari E, Bertocci B, et al. 8-oxo-guanine bypass by human DNA polymerases in the presence of auxiliary proteins. Nature. 2007;447:606-8 pubmed
  4. Markkanen E, Hubscher U, van Loon B. Regulation of oxidative DNA damage repair: the adenine:8-oxo-guanine problem. Cell Cycle. 2012;11:1070-5 pubmed publisher
    ..In contrast, phosphorylation of Pol ? by Cdk2/cyclinA counteracts this degradation by recruiting it to MutYH on chromatin to form active 8-oxo-G repair complexes. ..
  5. Garcia Diaz M, Bebenek K, LARREA A, Havener J, Perera L, Krahn J, et al. Template strand scrunching during DNA gap repair synthesis by human polymerase lambda. Nat Struct Mol Biol. 2009;16:967-72 pubmed publisher
    Family X polymerases such as DNA polymerase lambda (Pol lambda) are well suited for filling short gaps during DNA repair because they simultaneously bind both the 5' and 3' ends of short gaps...
  6. Crespan E, Hubscher U, Maga G. Error-free bypass of 2-hydroxyadenine by human DNA polymerase lambda with Proliferating Cell Nuclear Antigen and Replication Protein A in different sequence contexts. Nucleic Acids Res. 2007;35:5173-81 pubmed
    ..Our data show, for the first time, that the 2-OH-A lesion can be efficiently and faithfully bypassed by a human DNA pol lambda in combination with PCNA and RP-A. ..
  7. Lee J, Blanco L, Zhou T, Garcia Diaz M, Bebenek K, Kunkel T, et al. Implication of DNA polymerase lambda in alignment-based gap filling for nonhomologous DNA end joining in human nuclear extracts. J Biol Chem. 2004;279:805-11 pubmed
    ..The results suggest that polymerase lambda is the primary gap-filling polymerase for accurate nonhomologous end joining, and that the Brca1 C-terminal domain is required for this activity. ..
  8. Nagasawa K, Kitamura K, Yasui A, Nimura Y, Ikeda K, Hirai M, et al. Identification and characterization of human DNA polymerase beta 2, a DNA polymerase beta -related enzyme. J Biol Chem. 2000;275:31233-8 pubmed
    ..These results indicate that Pol beta2 is a Pol beta-related DNA polymerase with a BRCT motif that is dispensable for its polymerase activity. ..
  9. Garcia Diaz M, Bebenek K, Krahn J, Kunkel T, Pedersen L. A closed conformation for the Pol lambda catalytic cycle. Nat Struct Mol Biol. 2005;12:97-8 pubmed
  10. van Loon B, Hubscher U. An 8-oxo-guanine repair pathway coordinated by MUTYH glycosylase and DNA polymerase lambda. Proc Natl Acad Sci U S A. 2009;106:18201-6 pubmed publisher
    ..These results reveal a cellular response pathway to ROS, important to sustain genomic stability and modulate carcinogenesis. ..
  11. Brown J, Fiala K, Fowler J, Sherrer S, Newmister S, Duym W, et al. A novel mechanism of sugar selection utilized by a human X-family DNA polymerase. J Mol Biol. 2010;395:282-90 pubmed publisher
    ..In this study, we demonstrated that human DNA polymerase lambda used a novel sugar selection mechanism to discriminate against ribonucleotides, whereby the ribose 2'-..
  12. Maga G, Blanca G, Shevelev I, Frouin I, Ramadan K, Spadari S, et al. The human DNA polymerase lambda interacts with PCNA through a domain important for DNA primer binding and the interaction is inhibited by p21/WAF1/CIP1. FASEB J. 2004;18:1743-5 pubmed
    In this paper we show that DNA polymerase lambda (pol lambda) interacts with proliferating cell nuclear antigen (PCNA) in vivo in human cells...
  13. Garcia Diaz M, Bebenek K, Krahn J, Blanco L, Kunkel T, Pedersen L. A structural solution for the DNA polymerase lambda-dependent repair of DNA gaps with minimal homology. Mol Cell. 2004;13:561-72 pubmed
    Human DNA polymerase lambda (Pol lambda) is a family X member with low frameshift fidelity that has been suggested to perform gap-filling DNA synthesis during base excision repair and during repair of broken ends with limited homology...
  14. Garcia Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, et al. DNA polymerase lambda, a novel DNA repair enzyme in human cells. J Biol Chem. 2002;277:13184-91 pubmed
    b>DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair...
  15. Bebenek K, Garcia Diaz M, Blanco L, Kunkel T. The frameshift infidelity of human DNA polymerase lambda. Implications for function. J Biol Chem. 2003;278:34685-90 pubmed
    b>DNA polymerase lambda (Pol lambda) is a member of the Pol X family having properties in common with several other mammalian DNA polymerases...
  16. Blanca G, Shevelev I, Ramadan K, Villani G, Spadari S, Hubscher U, et al. Human DNA polymerase lambda diverged in evolution from DNA polymerase beta toward specific Mn(++) dependence: a kinetic and thermodynamic study. Biochemistry. 2003;42:7467-76 pubmed
    The recently discovered human DNA polymerase lambda (DNA pol lambda) has been implicated in translesion DNA synthesis across abasic sites...
  17. Blanca G, Villani G, Shevelev I, Ramadan K, Spadari S, Hubscher U, et al. Human DNA polymerases lambda and beta show different efficiencies of translesion DNA synthesis past abasic sites and alternative mechanisms for frameshift generation. Biochemistry. 2004;43:11605-15 pubmed
  18. Aoufouchi S, Flatter E, Dahan A, Faili A, Bertocci B, Storck S, et al. Two novel human and mouse DNA polymerases of the polX family. Nucleic Acids Res. 2000;28:3684-93 pubmed
    ..This suggests that their biological function may differ from DNA translesion synthesis, for which several DNA polymerase activities have been recently described. Possible functions are discussed. ..
  19. Bebenek K, Garcia Diaz M, Zhou R, Povirk L, Kunkel T. Loop 1 modulates the fidelity of DNA polymerase lambda. Nucleic Acids Res. 2010;38:5419-31 pubmed publisher
  20. Garcia Diaz M, Bebenek K, Kunkel T, Blanco L. Identification of an intrinsic 5'-deoxyribose-5-phosphate lyase activity in human DNA polymerase lambda: a possible role in base excision repair. J Biol Chem. 2001;276:34659-63 pubmed
    ..b>DNA polymerase lambda (Pol lambda) is a recently identified eukaryotic DNA polymerase that is homologous to Pol beta...
  21. Haracska L, Unk I, Johnson R, Phillips B, Hurwitz J, Prakash L, et al. Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA. Mol Cell Biol. 2002;22:784-91 pubmed
    Humans have three DNA polymerases, Poleta, Polkappa, and Poliota, which are able to promote replication through DNA lesions...
  22. Ramadan K, Maga G, Shevelev I, Villani G, Blanco L, Hubscher U. Human DNA polymerase lambda possesses terminal deoxyribonucleotidyl transferase activity and can elongate RNA primers: implications for novel functions. J Mol Biol. 2003;328:63-72 pubmed
    b>DNA polymerase lambda is a novel enzyme of the family X of DNA polymerases...
  23. Maga G, Shevelev I, Villani G, Spadari S, Hubscher U. Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda. Nucleic Acids Res. 2006;34:1405-15 pubmed
    b>DNA polymerase lambda (pol lambda) is a member of the X family DNA polymerases and is endowed with multiple enzymatic activities...
  24. Wimmer U, Ferrari E, Hunziker P, Hubscher U. Control of DNA polymerase lambda stability by phosphorylation and ubiquitination during the cell cycle. EMBO Rep. 2008;9:1027-33 pubmed publisher
    ..In particular, Pol lambda is stabilized during cell cycle progression in the late S and G2 phases. This most likely allows Pol lambda to correctly conduct repair of damaged DNA during and after S phase. ..
  25. Fiala K, Duym W, Zhang J, Suo Z. Up-regulation of the fidelity of human DNA polymerase lambda by its non-enzymatic proline-rich domain. J Biol Chem. 2006;281:19038-44 pubmed
    ..DNA polymerase beta plays a critical role in base-excision repair in vivo. DNA polymerase lambda, a recently identified X-family homolog of DNA polymerase beta, is hypothesized to be a second polymerase ..
  26. Lebedeva N, Rechkunova N, Dezhurov S, Khodyreva S, Favre A, Blanco L, et al. Comparison of functional properties of mammalian DNA polymerase lambda and DNA polymerase beta in reactions of DNA synthesis related to DNA repair. Biochim Biophys Acta. 2005;1751:150-8 pubmed
    b>DNA polymerase lambda (Pol lambda) is a novel enzyme of the family X of DNA polymerases. Pol lambda has some properties in common with DNA polymerase beta (Pol beta)...
  27. Lapadula G, Marchesoni A, Salaffi F, Ramonda R, Salvarani C, Punzi L, et al. Evidence-based algorithm for diagnosis and assessment in psoriatic arthritis: results by Italian DElphi in psoriatic Arthritis (IDEA). Reumatismo. 2016;68:126-136 pubmed publisher
    ..However, the elaborated algorithm needs to be further investigated in daily practice, for evidencing and proving its eventual efficacy in detecting and staging PsA and its heterogeneous spectrum appropriately. ..
  28. Burak M, Guja K, Hambardjieva E, Derkunt B, Garcia Diaz M. A fidelity mechanism in DNA polymerase lambda promotes error-free bypass of 8-oxo-dG. EMBO J. 2016;35:2045-59 pubmed publisher
    ..In order to maintain genomic integrity, post-replicative 8-oxo-dG:dA mispairs are removed through DNA polymerase lambda (Pol ?)-dependent MUTYH-initiated base excision repair (BER)...
  29. Gosavi R, Moon A, Kunkel T, Pedersen L, Bebenek K. The catalytic cycle for ribonucleotide incorporation by human DNA Pol ?. Nucleic Acids Res. 2012;40:7518-27 pubmed publisher
    ..The results are relevant to how ribonucleotides are incorporated into DNA in vivo, during replication and during repair, perhaps especially in non-proliferating cells when rNTP:dNTP ratios are high. ..
  30. Whaley Connell A, Habibi J, Nistala R, Hayden M, Pulakat L, Sinak C, et al. Combination of direct renin inhibition with angiotensin type 1 receptor blockade improves aldosterone but does not improve kidney injury in the transgenic Ren2 rat. Regul Pept. 2012;176:36-44 pubmed publisher
    ..Further, combination therapy did not improve markers of oxidative stress and podocyte and proximal tubule integrity in this transgenic model of RAAS-mediated kidney damage despite greater reductions in serum aldosterone and BP levels. ..
  31. Moghaddar N, Swan A, van der Werf J. Genomic prediction from observed and imputed high-density ovine genotypes. Genet Sel Evol. 2017;49:40 pubmed publisher
    ..2%) was observed for animals that were genetically lowly related to the reference set while it ranged from 0.0 to 5.0% for across-breed prediction. On average, no significant advantage was observed with BayesR compared to GBLUP. ..
  32. Hamilton A, Kandala N. Geography and correlates of attitude toward Female Genital Mutilation (FGM) in Sudan: What can we learn from successive Sudan opinion poll data?. Spat Spatiotemporal Epidemiol. 2016;16:59-76 pubmed publisher
    ..The decrease in the practice of cutting observed between the 2006 and 2010 Sudanese Household Surveys and the resulting shift in attitude make a compelling case for public health policy to eradicate the FGM practice. ..
  33. Ma X, Pan Q, Feng Y, Choudhury B, Ma Q, Gagneux P, et al. Sialylation Facilitates the Maturation of Mammalian Sperm and Affects Its Survival in Female Uterus. Biol Reprod. 2016;94:123 pubmed publisher
    ..Our results highlight the different mechanisms of increasing sialylation, which lead to the formation of the mature sperm sialome, as well as reveal the sialome's function in sperm survival within the female genital tract. ..
  34. Liu S, Gong H, Wang Y, Wang L. Label-free electrochemical nucleic acid biosensing by tandem polymerization and cleavage-mediated cascade target recycling and DNAzyme amplification. Biosens Bioelectron. 2016;77:818-23 pubmed publisher
  35. Rothman R, France C, Bykov V, de Costa B, Jacobson A, Woods J, et al. Pharmacological activities of optically pure enantiomers of the kappa opioid agonist, U50,488, and its cis diastereomer: evidence for three kappa receptor subtypes. Eur J Pharmacol. 1989;167:345-53 pubmed
    ..The order of potency was (-)-(1S,2S)-U50,488 greater than (+/-)-U50,488 greater than (+/-)-cis diastereomer of U50,488 greater than (+)-(1R,2R)-U50,488.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  36. Cranney S. Sex Life Satisfaction in Sub-Saharan Africa: A Descriptive and Exploratory Analysis. Arch Sex Behav. 2017;46:1961-1972 pubmed publisher
    ..In general, religiosity and perceived gravity of the HIV/AIDS problem in one's country were not significantly related to sexual satisfaction. ..
  37. van Loon B, Hubscher U, Maga G. Living on the Edge: DNA Polymerase Lambda between Genome Stability and Mutagenesis. Chem Res Toxicol. 2017;30:1936-1941 pubmed publisher
    ..Its multiple roles in DNA damage tolerance and its ability in promoting tumor progression make it also a possible target for novel anticancer approaches. ..
  38. Bruns N, Glenn I, McNinch N, Rosen M, Ponsky T. Treatment of routine adolescent inguinal hernia vastly differs between pediatric surgeons and general surgeons. Surg Endosc. 2017;31:912-916 pubmed publisher
  39. Partridge A, Carey L. Unmet Needs in Clinical Research in Breast Cancer: Where Do We Need to Go?. Clin Cancer Res. 2017;23:2611-2616 pubmed publisher
    ..i>Clin Cancer Res; 23(11); 2611-6. ©2017 AACRSee all articles in this CCR Focus section, "Breast Cancer Research: From Base Pairs to Populations." ..
  40. Shtygasheva A, Belousova E, Rechkunova N, Lebedeva N, Lavrik O. DNA polymerases beta and lambda as potential participants of TLS during genomic DNA replication on the lagging strand. Biochemistry (Mosc). 2008;73:1207-13 pubmed
    ..dATP was a preferential substrate for synthesis catalyzed by DNA polymerase beta. As for DNA polymerase lambda, dGMP was the only incorporated nucleotide out of four investigated...
  41. Zucca E, Bertoletti F, Wimmer U, Ferrari E, Mazzini G, Khoronenkova S, et al. Silencing of human DNA polymerase ? causes replication stress and is synthetically lethal with an impaired S phase checkpoint. Nucleic Acids Res. 2013;41:229-41 pubmed publisher
    ..Our results provide the first evidence, to our knowledge, that DNA pol ? is required for cell cycle progression and is functionally connected to the S phase DNA damage response machinery in cancer cells. ..
  42. Park Y, Ellis D, Mueller B, Stankowska D, Yorio T. Principles of Ocular Pharmacology. Handb Exp Pharmacol. 2017;242:3-30 pubmed publisher
    ..This chapter focuses on ocular pharmacology with specific emphasis on basic principles and outlining where in the various ocular sites are drug targets currently in use with effective drugs but also on future drug targets. ..
  43. Burak M, Guja K, Garcia Diaz M. Nucleotide binding interactions modulate dNTP selectivity and facilitate 8-oxo-dGTP incorporation by DNA polymerase lambda. Nucleic Acids Res. 2015;43:8089-99 pubmed publisher
    ..Because of its roles in oxidative DNA damage repair and non-homologous end joining, DNA polymerase lambda (Pol λ) may frequently encounter 8-oxo-dGTP...
  44. Wang S, He Z, Li D, Zhang B, Li M, Li W, et al. Aberrant methylation of RUNX3 is present in Aflatoxin B1-induced transformation of the L02R cell line. Toxicology. 2017;385:1-9 pubmed publisher
    ..Taken together, these observations suggest that aberrant methylation of RUNX3 and LINE-1 might be involved in AFB1-induced carcinogenesis. ..
  45. Salmela A, Lappalainen V, Reponen T, Pasanen P. Retention of fungal enzyme activity in environmental liquid and filter samples. Environ Sci Process Impacts. 2017;19:134-140 pubmed publisher
    ..Cladosporium spores had highest NAHA content per spore, whereas Acremonium did not exhibit any detectable enzyme activity even when viability was detected. The results indicated that samples should be stored as dry filter samples. ..
  46. Hyncicova E, Vyhnálek M, Kalina A, Martinkovic L, Nikolai T, Lisy J, et al. Cognitive impairment and structural brain changes in patients with clinically isolated syndrome at high risk for multiple sclerosis. J Neurol. 2017;264:482-493 pubmed publisher
  47. Crespan E, Hübscher U, Maga G. Expansion of CAG triplet repeats by human DNA polymerases λ and β in vitro, is regulated by flap endonuclease 1 and DNA ligase 1. DNA Repair (Amst). 2015;29:101-11 pubmed publisher
    ..These results may suggest a novel mechanism for somatic TNR expansion in HD. ..
  48. Nie Z, Li Z, Huang R, Liu Y, Li J, Wang L. A statistical frame based TDMA protocol for human body communication. Biomed Eng Online. 2015;14:65 pubmed publisher
    ..We also believe that our S-TDMA protocol will promote development of HBC in wearable applications. ..
  49. Rosenkrantz A, Hawkins C. Use of Twitter Polls to Determine Public Opinion Regarding Content Presented at a Major National Specialty Society Meeting. J Am Coll Radiol. 2017;14:177-182 pubmed publisher
    ..Twitter polls provide a free and easy infrastructure to potentially capture global public sentiment during the course of a medical society meeting. Their use may enrich and promote discussions of key session content. ..
  50. Nemec A, Bush K, Towle Weicksel J, Taylor B, Schulz V, Weidhaas J, et al. Estrogen Drives Cellular Transformation and Mutagenesis in Cells Expressing the Breast Cancer-Associated R438W DNA Polymerase Lambda Protein. Mol Cancer Res. 2016;14:1068-1077 pubmed
    Repair of DNA damage is critical for maintaining the genomic integrity of cells. DNA polymerase lambda (POLL/Pol λ) is suggested to function in base excision repair (BER) and nonhomologous end-joining (NHEJ), and is likely to play a ..
  51. Brown J, Duym W, Fowler J, Suo Z. Single-turnover kinetic analysis of the mutagenic potential of 8-oxo-7,8-dihydro-2'-deoxyguanosine during gap-filling synthesis catalyzed by human DNA polymerases lambda and beta. J Mol Biol. 2007;367:1258-69 pubmed
    ..kinetic methods, we examined human DNA polymerase beta and its novel X-family homolog, human DNA polymerase lambda, to determine which nucleotide and template base was preferred when encountering 8-oxodG and 8-oxodGTP, ..
  52. Chen Y, Jin L, Xue B, Jin D, Sun F, Wen C. NRAGE induces ?-catenin/Arm O-GlcNAcylation and negatively regulates Wnt signaling. Biochem Biophys Res Commun. 2017;487:433-437 pubmed publisher
    ..Therefore, our findings suggest a novel mechanism for regulating Wnt signaling. ..
  53. Diaz Parga P, Goto J, Krishnan V. Chemistry and Chemical Equilibrium Dynamics of BMAA and Its Carbamate Adducts. Neurotox Res. 2018;33:76-86 pubmed publisher
    ..The formation and the equilibrium process of carbamate adducts of BMAA may answer questions on their neuroactive potency and provide strong motivation for further investigations into other toxic mechanisms. ..
  54. Brown J, Pack L, Sherrer S, Kshetry A, Newmister S, Fowler J, et al. Identification of critical residues for the tight binding of both correct and incorrect nucleotides to human DNA polymerase ?. J Mol Biol. 2010;403:505-15 pubmed publisher
    ..The fidelity of Pol ? was maintained predominantly by a single residue, R517, which has minor groove interactions with the DNA template. ..
  55. Zhang Z, Tang R, Zhu D, Wang W, Yi L, Ma L. Non-peptide guided auto-secretion of recombinant proteins by super-folder green fluorescent protein in Escherichia coli. Sci Rep. 2017;7:6990 pubmed publisher
    ..This is the first report that sfGFP can guide the secretion of recombinant proteins out of the cells from cytoplasm in E. coli without affecting their conformation and function. ..
  56. Clift C, Salisbury D. Enhancing the role of vaccines in combatting antimicrobial resistance. Vaccine. 2017;35:6591-6593 pubmed publisher
    ..More evidence is needed to convince policymakers but that vaccine development projects should be considered by funders on the same basis as those for new antibiotics or diagnostics. ..
  57. Niida H, Nakanishi M. [A novel mammalian DNA polymerase: DNA polymerase lambda (pol lambda)]. Seikagaku. 2002;74:224-6 pubmed
  58. Pande P, Haraguchi K, Jiang Y, Greenberg M, Basu A. Unlike catalyzing error-free bypass of 8-oxodGuo, DNA polymerase λ is responsible for a significant part of Fapy·dG-induced G → T mutations in human cells. Biochemistry. 2015;54:1859-62 pubmed publisher
  59. Starostenko L, Rechkunova N, Lebedeva N, Kolbanovskiy A, Geacintov N, Lavrik O. Human DNA polymerases catalyze lesion bypass across benzo[a]pyrene-derived DNA adduct clustered with an abasic site. DNA Repair (Amst). 2014;24:1-9 pubmed publisher
    ..The results suggest that Polλ is a specialized polymerase that can process these kinds of lesions. ..
  60. Hoeller D, Hecker C, Wagner S, Rogov V, Dötsch V, Dikic I. E3-independent monoubiquitination of ubiquitin-binding proteins. Mol Cell. 2007;26:891-8 pubmed
    ..This modification is mechanistically and functionally distinct from E3-mediated and growth factor-dependent monoubiquitination. ..
  61. De Matteis L, Martín Rapún R, de la Fuente J. Nanotechnology in Personalized Medicine: A Promising Tool for Alzheimer's Disease Treatment. Curr Med Chem. 2017;: pubmed publisher
  62. Martín M, García Ortiz M, Gomez Bedoya A, Esteban V, Guerra S, Blanco L. A specific N-terminal extension of the 8 kDa domain is required for DNA end-bridging by human Pol? and Pol?. Nucleic Acids Res. 2013;41:9105-16 pubmed publisher
  63. Belousova E, Maga G, Fan Y, Kubareva E, Romanova E, Lebedeva N, et al. DNA polymerases beta and lambda bypass thymine glycol in gapped DNA structures. Biochemistry. 2010;49:4695-704 pubmed publisher
    ..The results show that (i) DNA polymerase lambda is able to catalyze DNA synthesis across Tg, (ii) the ability of DNA polymerase lambda to elongate from a ..
  64. Crespan E, Furrer A, Rösinger M, Bertoletti F, Mentegari E, Chiapparini G, et al. Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair. Nat Commun. 2016;7:10805 pubmed publisher
    ..Studies in Pol β- and λ-deficient cell extracts suggest that Pol β levels can greatly affect rNMP incorporation opposite oxidative DNA lesions. ..
  65. Goldman M, Fee M. Computational training for the next generation of neuroscientists. Curr Opin Neurobiol. 2017;46:25-30 pubmed publisher
    ..Motivated by this survey, we suggest steps to facilitate quantitative and computational training for future neuroscientists. ..
  66. Demogines A, East A, Lee J, Grossman S, Sabeti P, Paull T, et al. Ancient and recent adaptive evolution of primate non-homologous end joining genes. PLoS Genet. 2010;6:e1001169 pubmed publisher
    ..We propose that an ongoing evolutionary arms race between viruses and NHEJ genes may be driving the surprisingly rapid evolution of these critical genes...
  67. Fowler J, Brown J, Kvaratskhelia M, Suo Z. Probing conformational changes of human DNA polymerase lambda using mass spectrometry-based protein footprinting. J Mol Biol. 2009;390:368-79 pubmed publisher
    Crystallographic studies of the C-terminal DNA polymerase-beta-like domain of full-length human DNA polymerase lambda (fPollambda) suggested that the catalytic cycle might not involve a large protein domain rearrangement as observed with ..
  68. Shimazaki N, Yoshida K, Kobayashi T, Toji S, Tamai K, Koiwai O. Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Genes Cells. 2002;7:639-51 pubmed
    b>DNA polymerase lambda (Pol lambda) was recently identified as a new member of the family X of DNA polymerases in eukaryotic cells...
  69. Becker A, Lucka L, Kilian C, Kannicht C, Reutter W. Characterisation of the ATP-dependent taurocholate-carrier protein (gp110) of the hepatocyte canalicular membrane. Eur J Biochem. 1993;214:539-48 pubmed
    ..In contrast, Fab fragments of these antibodies inhibit the aggregation of freshly isolated hepatocytes...
  70. He W, Lin G, Liang P, Cheng D, Hu X, Zhou L, et al. [Genetic analysis of three families affected with split-hand/split-foot malformation]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2017;34:476-480 pubmed publisher
    ..However, next-generation sequencing did not identify the breakpoint. The genetic etiology for three families affected with SHFM have been identified, which has provideda basis for genetic counseling and guidance for reproduction. ..
  71. Conroy S, Courneya K, Brenner D, Shaw E, O Reilly R, Yasui Y, et al. Impact of aerobic exercise on levels of IL-4 and IL-10: results from two randomized intervention trials. Cancer Med. 2016;5:2385-97 pubmed publisher
    ..Results from these two large randomized aerobic exercise intervention trials suggest that aerobic exercise does not alter IL-10 or IL-4 in a manner consistent with chronic disease and cancer prevention. ..
  72. Crespan E, Czabany T, Maga G, Hubscher U. Microhomology-mediated DNA strand annealing and elongation by human DNA polymerases ? and ? on normal and repetitive DNA sequences. Nucleic Acids Res. 2012;40:5577-90 pubmed publisher
    ..We propose a possible novel role of DNA pol ? in MMEJ, promoting (CAG)n triplet repeats instability. ..
  73. Antczak N, Packer M, Lu X, Zhang K, Beuning P. Human Y-Family DNA Polymerase ? Is More Tolerant to Changes in Its Active Site Loop than Its Ortholog Escherichia coli DinB. Chem Res Toxicol. 2017;30:2002-2012 pubmed publisher
    ..Overall we find that pol ? is more tolerant to changes in the active site loop than DinB. ..
  74. Skosareva L, Lebedeva N, Rechkunova N, Kolbanovskiy A, Geacintov N, Lavrik O. Human DNA polymerase ? catalyzes lesion bypass across benzo[a]pyrene-derived DNA adduct during base excision repair. DNA Repair (Amst). 2012;11:367-73 pubmed publisher
    ..The capability of Pol? to introduce the correct dNTP opposite the cis-BP-dG adduct in gap filling reactions suggests that this polymerase may play a specialized role in the process of repair of these kinds of lesions. ..
  75. Stevens A, Guan L, Bebenek K, Kunkel T, Greenberg M. DNA polymerase ? inactivation by oxidized abasic sites. Biochemistry. 2013;52:975-83 pubmed publisher
    ..The ability of DOB and pC4-AP to inactivate Pol ? provides additional evidence that these lesions are significant sources of the cytotoxicity of DNA-damaging agents that produce them. ..
  76. Klindt D, Devaine M, Daunizeau J. Does the way we read others' mind change over the lifespan? Insights from a massive web poll of cognitive skills from childhood to late adulthood. Cortex. 2017;86:205-215 pubmed publisher
    ..In particular, this implies that, over the lifespan, people may rely upon distinct cognitive architectures when reading others' minds. ..