DHRS9

Summary

Gene Symbol: DHRS9
Description: dehydrogenase/reductase 9
Alias: 3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4, dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, NADP-dependent retinol dehydrogenase/reductase, dehydrogenase/reductase (SDR family) member 9, retinol dehydrogenase 15, retinol dehydrogenase homolog, short chain dehydrogenase/reductase family 9C member 4, short-chain dehydrogenase/reductase retSDR8, tracheobronchial epithelial cell-specific retinol dehydrogenase
Species: human

Top Publications

  1. ncbi Molecular and metabolic retinoid pathways in the human ocular surface
    Hachemi Nezzar
    Universite d Auvergne, JE 2447, ARDEMO, France
    Mol Vis 13:1641-50. 2007
  2. ncbi Tetrahydrobiopterin is synthesized from 6-pyruvoyl-tetrahydropterin by the human aldo-keto reductase AKR1 family members
    Teruhiko Iino
    Department of General Studies, Nihon University, Sakurajosui 3 25 40, Setagaya Ku, Tokyo 156 8550, Japan
    Arch Biochem Biophys 416:180-7. 2003
  3. ncbi Understanding oligomerization in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni: an in silico approach and evidence for an active protein
    Frank Hoffmann
    Department of Pharmacology and Toxicology, Philipps University Marburg, Karl v Frisch Str 1, D 35033 Marburg, Germany
    J Biotechnol 129:131-9. 2007
  4. ncbi Purification and properties of 3 alpha-hydroxysteroid dehydrogenase as a 3-keto bile acid reductase from human liver cytosol
    K Kudo
    Third Department of Internal Medicine, Hyogo College of Medicine, Japan
    Biochim Biophys Acta 1046:12-8. 1990
  5. ncbi Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Chi Ching Hwang
    Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan
    J Biol Chem 280:3522-8. 2005
  6. ncbi 5 alpha-reductase activity in the human hair follicle concentrates in the dermal papilla
    W Eicheler
    Philipp University, Department of Dermatology, Marburg, Germany
    Arch Dermatol Res 290:126-32. 1998
  7. ncbi Kinetics of allopregnanolone formation catalyzed by human 3 alpha-hydroxysteroid dehydrogenase type III (AKR1C2)
    John W Trauger
    Department of Molecular Neuroscience, Merck Research Laboratories, 3535 General Atomics Court, San Diego, California 92121, USA
    Biochemistry 41:13451-9. 2002
  8. ncbi Alanine scanning mutagenesis of the testosterone binding site of rat 3 alpha-hydroxysteroid dehydrogenase demonstrates contact residues influence the rate-determining step
    Vladi V Heredia
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Biochemistry 43:5832-41. 2004
  9. ncbi Dissection of the physiological interconversion of 5alpha-DHT and 3alpha-diol by rat 3alpha-HSD via transient kinetics shows that the chemical step is rate-determining: effect of mutating cofactor and substrate-binding pocket residues on catalysis
    Vladi V Heredia
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Biochemistry 43:12028-37. 2004
  10. ncbi Identification of the oxidative 3alpha-hydroxysteroid dehydrogenase activity of rat Leydig cells as type II retinol dehydrogenase
    D O Hardy
    Population Council and The Rockefeller University, New York, New York 10021, USA
    Endocrinology 141:1608-17. 2000

Research Grants

  1. INHIBITION OF 3A HSD BY ANTI-INFLAMMATORY DRUGS
    TREVOR PENNING; Fiscal Year: 1991
  2. Symposium on Aldo-Keto Reductases & Toxicant Metabolism
    TREVOR PENNING; Fiscal Year: 2002
  3. Molecular Mechanisms of Multistage Carcinogenesis
    TREVOR PENNING; Fiscal Year: 2006
  4. Northeast Under/Grad Research Organization for Neuroscience
    Cheryl Frye; Fiscal Year: 2008
  5. PHARMACOLOGY--ALLOSTERIC MODULATORS OF GABAA RECEPTORS
    Alessandro Guidotti; Fiscal Year: 2002
  6. REELIN DEFICIENCY--MODEL FOR SCHIZOPHRENIA VULNERABILITY
    Alessandro Guidotti; Fiscal Year: 2004
  7. REGULATION OF REPRODUCTION
    Synthia Mellon; Fiscal Year: 2005
  8. PATHOLOGY OF THE LABORATORY MOUSE
    John Sundberg; Fiscal Year: 2006
  9. RETINOID METABOLISM
    JOSEPH NAPOLI; Fiscal Year: 2007
  10. Biosynthesis of 9-cis-retinoic acid/retinol homeostasis
    JOSEPH NAPOLI; Fiscal Year: 2007

Scientific Experts

Detail Information

Publications140 found, 100 shown here

  1. ncbi Molecular and metabolic retinoid pathways in the human ocular surface
    Hachemi Nezzar
    Universite d Auvergne, JE 2447, ARDEMO, France
    Mol Vis 13:1641-50. 2007
    ..The second objective was to demonstrate the presence of different enzymes transforming vitamin A to retinoic acid and the functionality of this metabolic pathway in the corneal epithelium...
  2. ncbi Tetrahydrobiopterin is synthesized from 6-pyruvoyl-tetrahydropterin by the human aldo-keto reductase AKR1 family members
    Teruhiko Iino
    Department of General Studies, Nihon University, Sakurajosui 3 25 40, Setagaya Ku, Tokyo 156 8550, Japan
    Arch Biochem Biophys 416:180-7. 2003
    ....
  3. ncbi Understanding oligomerization in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni: an in silico approach and evidence for an active protein
    Frank Hoffmann
    Department of Pharmacology and Toxicology, Philipps University Marburg, Karl v Frisch Str 1, D 35033 Marburg, Germany
    J Biotechnol 129:131-9. 2007
    ..In addition, despite lacking the extra loop domain, mutant 3alpha-HSD/CR was shown to be active towards a variety of standard substrates...
  4. ncbi Purification and properties of 3 alpha-hydroxysteroid dehydrogenase as a 3-keto bile acid reductase from human liver cytosol
    K Kudo
    Third Department of Internal Medicine, Hyogo College of Medicine, Japan
    Biochim Biophys Acta 1046:12-8. 1990
    ..The enzyme activity was inhibited by p-chloromercuribenzoate; however, the inhibition was prevented by addition of dithiothreitol to the reaction mixture, indicating that the enzyme required a sulfhydryl group for activity...
  5. ncbi Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Chi Ching Hwang
    Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan
    J Biol Chem 280:3522-8. 2005
    ....
  6. ncbi 5 alpha-reductase activity in the human hair follicle concentrates in the dermal papilla
    W Eicheler
    Philipp University, Department of Dermatology, Marburg, Germany
    Arch Dermatol Res 290:126-32. 1998
    ..These results indicate that the androgen response of hair follicles depends on a differentiated intrafollicular androgen metabolism and that the dermal papilla might be a primary target in this process...
  7. ncbi Kinetics of allopregnanolone formation catalyzed by human 3 alpha-hydroxysteroid dehydrogenase type III (AKR1C2)
    John W Trauger
    Department of Molecular Neuroscience, Merck Research Laboratories, 3535 General Atomics Court, San Diego, California 92121, USA
    Biochemistry 41:13451-9. 2002
    ..These results characterize the role of 3alpha-HSD type III in allopregnanolone formation and suggest that activation of this enzyme by fluoxetine is likely not the mechanism by which fluoxetine increases allopregnanolone concentrations...
  8. ncbi Alanine scanning mutagenesis of the testosterone binding site of rat 3 alpha-hydroxysteroid dehydrogenase demonstrates contact residues influence the rate-determining step
    Vladi V Heredia
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Biochemistry 43:5832-41. 2004
    ..These findings support the concept of enzyme catalysis in which the correct positioning of reactants is essential; otherwise, k(cat) will be limited by the chemical event...
  9. ncbi Dissection of the physiological interconversion of 5alpha-DHT and 3alpha-diol by rat 3alpha-HSD via transient kinetics shows that the chemical step is rate-determining: effect of mutating cofactor and substrate-binding pocket residues on catalysis
    Vladi V Heredia
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Biochemistry 43:12028-37. 2004
    ..Thus, residues in the cofactor and steroid-binding site can alter the rate-determining step in the NADP(+)-dependent oxidation of 3alpha-diol to make chemistry rate-limiting overall...
  10. ncbi Identification of the oxidative 3alpha-hydroxysteroid dehydrogenase activity of rat Leydig cells as type II retinol dehydrogenase
    D O Hardy
    Population Council and The Rockefeller University, New York, New York 10021, USA
    Endocrinology 141:1608-17. 2000
    ..We conclude that type I 3alpha-HSD and RoDH2 have distinct 3alpha-HSD activities with opposing catalytic directions, thereby controlling the rates of DHT production by Leydig cells...
  11. ncbi 3(20)alpha-hydroxysteroid dehydrogenase activity of monkey liver indanol dehydrogenase
    A Hara
    Department of Biochemistry, Gifu Pharmaceutical University
    J Biochem 106:900-3. 1989
    ..The results strongly suggest that indanol dehydrogenase acts as a 3(20)alpha-hydroxysteroid dehydrogenase in the metabolism of certain steroid hormones and bile acids...
  12. ncbi Sex, drugs and sports: prostaglandins, epitestosterone and sexual development
    Bryan K Sanders
    College of Letters and Science, University of California, Berkeley, CA 94720, USA
    Med Hypotheses 69:829-35. 2007
    ..Given the devastating personal and career consequences that may result from false positive drug tests, substantive research on the effects of PGE2 manipulations on EpiT is warranted...
  13. doi New therapeutic approaches for epilepsies, focusing on reorganization of the GABAA receptor subunits by neurosteroids
    Yoshiya L Murashima
    Graduate School of Human Health Science, Tokyo Metropolitan University, Tokyo, Japan
    Epilepsia 51:131-4. 2010
    ..Therefore, GABAA receptor-regulating neurosteroids may be a promising target for the development of novel antiepileptic agents...
  14. ncbi Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor. Role of recombinant cytochrome b5 and P450 reductase
    Penny Soucy
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Center CHUL and Laval University, Quebec, Canada
    Eur J Biochem 270:1349-55. 2003
    ..Our results therefore demonstrate that human P450c17, as other enzymes of the classical steroidogenic pathway, is involved in the biosynthetic pathway leading to the formation of androstenol...
  15. ncbi Proteome analysis of rat hepatomas: carcinogen-dependent tumor-associated protein variants
    E Zeindl-Eberhart
    Institute of Pathology, University of Munich, Germany
    Electrophoresis 22:3009-18. 2001
    ..The hypothesis is proposed that carcinogens leave specific fingerprints at the proteome level of manifest liver tumors...
  16. ncbi Isolation and partial characterization of a full-length cDNA clone for 3 alpha-hydroxysteroid dehydrogenase: a potential target enzyme for nonsteroidal anti-inflammatory drugs
    J Pawlowski
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Agents Actions 34:289-93. 1991
    ..These findings support the role 3 alpha-HSD in inflammation and suggest that hydroxysteroid dehydrogenases, hydroxyprostaglandin dehydrogenases and prostaglandin F2 alpha synthase may be members of a common gene family...
  17. ncbi Fluorescence high performance liquid chromatographic determination of 3 alpha-hydroxysteroids in urine of 21-hydroxylase deficiency
    T Kawasaki
    School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Biomed Chromatogr 1:1-6. 1986
    ..Each steroid was measured at the 2.5 micrograms/dl at the highest sensitivity of the detector. The mean recoveries and reproducibilities were 91.5-108.2% with 0.9-6.5% (CV%)...
  18. ncbi Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3 alpha-hydroxysteroid dehydrogenases
    M Khanna
    Department of Pediatrics, Cornell University Medical College, New York, New York 10021, USA
    J Biol Chem 270:20162-8. 1995
    ..The type I gene contains a TATA box that is located 27 bp upstream of multiple transcription start sites. In contrast, the type II gene contains two tandem AP2 sequences juxtaposed to a single transcription start site...
  19. ncbi Genomic structure of rat 3alpha-hydroxysteroid/dihydrodiol dehydrogenase (3alpha-HSD/DD, AKR1C9)
    H K Lin
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084, USA
    J Steroid Biochem Mol Biol 71:29-39. 1999
    ..It is unknown whether the type 2 and type 3 3alpha-HSD/DD genes represent pseudo-genes or whether they represent genes that are differentially expressed in other rat tissues...
  20. ncbi Inhibition of 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD) activity of human lung microsomes by genistein, daidzein, coumestrol and C(18)-, C(19)- and C(21)-hydroxysteroids and ketosteroids
    Charles H Blomquist
    Department of Obstetrics and Gynecology, HealthPartners Regions Hospital, St Paul, MN 55101, USA
    Steroids 70:507-14. 2005
    ..The observation of a mixed inhibition pattern suggests these compounds bind to more than one form of the enzyme within the catalytic pathway...
  21. ncbi Molecular cloning and expression of rat liver 3 alpha-hydroxysteroid dehydrogenase
    K C Cheng
    Department of Pediatrics Cornell University Medical College, New York, New York 10021
    Mol Endocrinol 5:823-8. 1991
    Complementary DNA clones encoding 3 alpha-hydroxysteroid dehydrogenase (3 alpha HSD) were isolated from a rat liver cDNA lambda gt11 expression library using monoclonal antibodies as probes. The sizes of the cDNA inserts ranged from 1...
  22. ncbi Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenases from rat and human prostate
    M G Biswas
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9046, USA
    J Biol Chem 272:15959-66. 1997
    ..The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated...
  23. ncbi Isolation and characterization of a cDNA encoding mouse 3alpha-hydroxysteroid dehydrogenase: an androgen-inactivating enzyme selectively expressed in female tissues
    Véronique Bellemare
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL, 2705 Laurier Boulevard, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 98:18-24. 2006
    ..This role could be related to the inactivation of excess of androgen and progesterone that are more severely regulated than in man...
  24. ncbi Enzymatic properties of a member (AKR1C20) of the aldo-keto reductase family
    Kengo Matsumoto
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Japan
    Biol Pharm Bull 29:539-42. 2006
    ..Thus, AKR1C20 is a novel 3alpha(17beta)-HSD, which may also function as a reductase for xenobiotic alpha-dicarbonyl compounds...
  25. ncbi 5 alpha-reduced androgens play a key role in murine parturition
    M S Mahendroo
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235 9046, USA
    Mol Endocrinol 10:380-92. 1996
    ..The results document a role for 5 alpha-reduced androgens synthesized by the type 1 isozyme in normal female physiology, and they suggest that 3 alpha-Adiol is a new hormone required for parturition in mice...
  26. doi Cis- and trans-regulatory elements of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase as biosensor system for steroid determination in the environment
    Guangming Xiong
    Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig Holstein, Kiel, Germany
    Chem Biol Interact 178:215-20. 2009
    ..testosteroni strain that can be used as a sensitive biosensor system for steroid determination in the environment...
  27. ncbi Molecular structure of rat hepatic 3 alpha-hydroxysteroid dehydrogenase. A member of the oxidoreductase gene family
    A Stolz
    Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine 90033
    J Biol Chem 266:15253-7. 1991
    ..The significant homology of 3 alpha-HSD with both prostaglandin F synthetase and aldose reductases suggest a subdivision of monomeric, NADPH reductases within the larger oxidoreductases superfamily...
  28. ncbi Affinity labeling of 3 alpha-hydroxysteroid dehydrogenase with 3 alpha-bromoacetoxyandrosterone and 11 alpha-bromoacetoxyprogesterone. Isolation and sequence of active site peptides containing reactive cysteines; sequence confirmation using nucleotide seq
    T M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104
    J Biol Chem 266:8826-34. 1991
    ..By contrast the 11 alpha-bromoacetoxy group in Br11P labeled several reactive cysteines which may be involved in the binding of glucocorticoids and NSAIDs...
  29. ncbi Effect of the bile-acid sequestrant colestipol on postprandial serum bile-acid concentration: evaluation by bioluminescent enzymic analysis
    S S Rossi
    Department of Medicine, UC San Diego, La Jolla 92093
    Aliment Pharmacol Ther 3:41-6. 1989
    ..The data suggest that measurement of serum bile-acid concentrations by bioluminescence is a rapid, simple way to document the efficacy of bile-acid sequestrants...
  30. ncbi Potentiation of myeloid differentiation by anti-inflammatory agents, by steroids and by retinoic acid involves a single intracellular target, probably an enzyme of the aldoketoreductase family
    C M Bunce
    Department of Immunology, University of Birmingham, Edgbaston, UK
    Biochim Biophys Acta 1311:189-98. 1996
    ....
  31. pmc Selective serotonin reuptake inhibitors directly alter activity of neurosteroidogenic enzymes
    L D Griffin
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, Box 0556, San Francisco, CA 94143 0556, USA
    Proc Natl Acad Sci U S A 96:13512-7. 1999
    ..Our results may thus help explain the rapid alleviation of the anxiety and dysphoria associated with late luteal phase dysphoria disorder and major unipolar depression by these SSRIs...
  32. ncbi Interactions between type 1 astrocytes and LHRH-secreting neurons (GT1-1 cells): modification of steroid metabolism and possible role of TGFbeta1
    I Cavarretta
    Department of Endocrinology, University of Milan, Italy
    J Steroid Biochem Mol Biol 71:41-7. 1999
    ..The present findings underline once more the importance of the physiological cross-talk between A1 and neurons...
  33. ncbi Pathogenesis of diabetic neuropathy--do hyperglycemia and aldose reductase inhibitors affect neuroactive steroid formation in the rat sciatic nerves?
    A Colciago
    Department of Endocrinology, University of Milano, Italy
    Exp Clin Endocrinol Diabetes 110:22-6. 2002
    ..We conclude that hyperglycemia or the administration of the AR inhibitors do not affect 3alpha-HSD activity in peripheral nerves and therefore do not reduce the formation of steroid metabolites possibly endowed with neurotrophic action...
  34. ncbi Androgenic and estrogenic 17beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase in human ovarian epithelial tumors: evidence for the type 1, 2 and 5 isoforms
    Charles H Blomquist
    Department of Obstetrics and Gynecology, Health Partners Regions Hospital, 640 Jackson Street, St Paul, MN 55101 2595, USA
    J Steroid Biochem Mol Biol 81:343-51. 2002
    ....
  35. ncbi Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4
    Elena A Lapshina
    Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, Kansas City, Missouri 64110, USA
    Biochemistry 42:776-84. 2003
    ....
  36. ncbi 13-cis-retinoic acid competitively inhibits 3 alpha-hydroxysteroid oxidation by retinol dehydrogenase RoDH-4: a mechanism for its anti-androgenic effects in sebaceous glands?
    Teresa Karlsson
    Department of Medical Sciences Dermatology and Venereology, University Hospital, Uppsala, Sweden
    Biochem Biophys Res Commun 303:273-8. 2003
    ..e., reduced the formation of dihydrotestosterone and androstandione in vitro. Extrapolated to the in vivo situation, this effect might explain the unique sebosuppressive effect of isotretinoin when treating acne...
  37. ncbi The 3alpha-hydroxy-steroid-dehydrogenase-mRNA and -protein are more prevalent in pericentral than in periportal hepatocytes
    Ulrich Baumgartner
    Department of General Surgery, University of Freiburg, Germany
    Eur J Gastroenterol Hepatol 15:509-13. 2003
    ..Because bile acid processing is greater in the pericentral than in the periportal region, we investigated whether 3alpha-HSD is more prevalent in the pericentral than in the periportal area...
  38. ncbi Kinetic study of the enzymatic cycling reaction conducted with 3alpha-hydroxysteroid dehydrogenase in the presence of excessive thio-NAD(+) and NADH
    Shigeru Ueda
    Department of Diagnostics Research and Development, Division of Fine Chemicals and Diagnostics, Asahi Kasei Pharma Corporation, 632 1, Mifuku, Ohito cho, Shizuoka 410 2321, Japan
    Anal Biochem 332:84-9. 2004
    ..The cycling rate was verified by a comparison with the experimental data using 3alpha-HSD from Pseudomonas sp. B-0831. The results showed that the experimental data corresponded well with the results obtained from the kinetic model...
  39. ncbi Selective loss of AKR1C1 and AKR1C2 in breast cancer and their potential effect on progesterone signaling
    Qing Ji
    Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Cancer Res 64:7610-7. 2004
    ..We speculate that loss of AKR1C1 and AKR1C2 in breast cancer results in decreased progesterone catabolism, which, in combination with increased PR expression, may augment progesterone signaling by its nuclear receptors...
  40. ncbi Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Dept of Nutrition, Laval University Medical Center, 2705 Laurier Boulevard, Rm T3 67, Quebec City, Prov Quebec, Canada G1V 4G2
    Am J Physiol Endocrinol Metab 288:E398-404. 2005
    ..Increased progesterone and/or dihydrotestosterone reduction in abdominal adipose tissue may impact locally on fat cell metabolism...
  41. ncbi Increased expression of type 2 3alpha-hydroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase (AKR1C3) and its relationship with androgen receptor in prostate carcinoma
    K M Fung
    Department of Urology, University of Oklahoma Health Sciences Center, 920 Stanton L Young Blvd, WP3150, Oklahoma City, OK 73104, USA
    Endocr Relat Cancer 13:169-80. 2006
    ..Although the biological significance of elevated AKR1C3 in prostatic carcinoma is uncertain, AKR1C3 may be responsible for the trophic effects of androgens and/or PGs on prostatic epithelial cells...
  42. pmc Crystallization and preliminary X-ray analysis of the complex of NADH and 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831
    Sachiyo Kataoka
    Graduate School of Agriculture, Kyoto Prefectural University, 1 5 Hangi cho, Shimogamo, Sakyo ku, Kyoto, Kyoto 606 8522, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:569-71. 2006
    ..8 A. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 62.46, b = 82.25, c = 86.57 A, and contained two molecules, reflecting dimer formation of 3alpha-HSD, in the asymmetric unit...
  43. doi Neurosteroid biosynthesis regulates sexually dimorphic fear and aggressive behavior in mice
    Graziano Pinna
    Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, 1601W Taylor Street, Chicago, IL 60612, USA
    Neurochem Res 33:1990-2007. 2008
    ....
  44. pmc MN1 affects expression of genes involved in hematopoiesis and can enhance as well as inhibit RAR/RXR-induced gene expression
    Magda A Meester-Smoor
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Carcinogenesis 29:2025-34. 2008
    ..g. MEIS1 and BMI1). Another interesting target is DHRS9. DHRS9 is involved in the synthesis of ATRA from vitamin A...
  45. pmc Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture
    Seung Ah Lee
    Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 286:13550-60. 2011
    ..4 (RoDH4, SDR9C8), RoDH-like 3α-hydroxysteroid dehydrogenase (RL-HSD, SDR9C6), and RDH-like SDR (RDHL, SDR9C4) do not affect the expression of retinoic acid-inducible genes but alter the expression levels of several ..
  46. pmc The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic genes in Leydig cells
    María Eugenia Matzkin
    Department of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, IA, United States
    Mol Cell Endocrinol 370:130-7. 2013
    ..of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic ..
  47. pmc Downregulation of DHRS9 expression in colorectal cancer tissues and its prognostic significance
    Liang Hu
    Anal Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China
    Tumour Biol 37:837-45. 2016
    Dehydrogenase/reductase (SDR family) member 9 (DHRS9) is aberrantly expressed in colorectal cancer (CRC), but its prognostic value is unknown. The aim of the work was to investigate the prognostic significance of DHRS9 expression in CRC...
  48. ncbi Testosterone and progesterone metabolism in the human neuroblastoma cell line SH-SY5Y
    R C Melcangi
    Department of Endocrinology, University of Milano, Italy
    J Steroid Biochem Mol Biol 46:811-8. 1993
    ..It is suggested that the SH-SY5Y cell line may represent a useful "in vitro" model for the study of the mechanisms involved in the control of androgen and P metabolism in nervous cells...
  49. ncbi cDNA cloning and expression of the human hepatic bile acid-binding protein. A member of the monomeric reductase gene family
    A Stolz
    Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine, Los Angeles 90033
    J Biol Chem 268:10448-57. 1993
    ..Future studies will define the physiologic significance of this novel, multifunctional protein in bile acid transport and xenobiotic metabolism...
  50. ncbi Expression and characterization of recombinant type 2 3 alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3 alpha/17 beta-HSD activity and cellular distribution
    H K Lin
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084, USA
    Mol Endocrinol 11:1971-84. 1997
    ..This study is the first complete characterization of recombinant type 2 3alpha-HSD demonstrating dual activity and cellular distribution in the human prostate...
  51. pmc Proposed mechanism for sperm chromatin condensation/decondensation in the male rat
    John C Chapman
    Dept Biological Sciences, Binghamton University, Binghamton, NY 13902 6000, USA
    Reprod Biol Endocrinol 1:20. 2003
    ..If our proposed mechanism for chromatin condensation/decondensation is borne out by further research, IVF clinics might consider preincubating spermatozoa with 5alpha-DHT in order to increase the efficiency of fertilization...
  52. ncbi Gender differences in the regulation of 3 alpha-hydroxysteroid dehydrogenase in rat brain and sensitivity to neurosteroid-mediated stress protection
    Y A Mitev
    Male Health Care II, Schering AG Jenapharm, Otto Schott Strasse 15, 07745 Jena, Germany
    Neuroscience 120:541-9. 2003
    ....
  53. ncbi Characterization and modulation of sex steroid metabolizing activity in normal human keratinocytes in primary culture and HaCaT cells
    Sebastien Gingras
    Molecular Endocrinology and Oncology Research Center, CHUL Research Center and Laval University, Quebec City, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 87:167-79. 2003
    ..The present data thus indicate that HaCaT cells are a useful model to further study the regulation of the enzymes involved in the metabolism of sex steroids in keratinocytes...
  54. ncbi Crystal structure of human prostaglandin F synthase (AKR1C3)
    Junichi Komoto
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045 7534, USA
    Biochemistry 43:2188-98. 2004
    ..Since the substrate binding cavity of PGFS is relatively large in comparison with those of AKR1C1 and AKR1C2, PGFS (AKR1C3) could catalyze the reduction and/or oxidation reactions of various compounds over a relatively wide pH range...
  55. ncbi New tools for molecular imaging of redox metabolism: development of a fluorogenic probe for 3 alpha-hydroxysteroid dehydrogenases
    Dominic J Yee
    Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027, USA
    J Am Chem Soc 126:2282-3. 2004
    ..25 min-1, Figure 4), a likely physiological substrate in prostate, revealed that synthetic probe 5 is in fact a far better substrate for this enzyme. Structure 5 represents an exciting lead for the development of a redox imaging probe...
  56. ncbi Anatomical and cellular localization of neuroactive 5 alpha/3 alpha-reduced steroid-synthesizing enzymes in the spinal cord
    Christine Patte-Mensah
    Laboratoire de Neurophysiologie Cellulaire et Intégrée, Unité Mixte de Recherche 7519 Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67084 Strasbourg, France
    J Comp Neurol 477:286-99. 2004
    ..These results are the first demonstrating that oligodendrocytes and neurons of the SC possess the key enzymatic complex for synthesizing potent neuroactive steroids that may control spinal sensorimotor processes...
  57. ncbi Adenomatous polyposis coli control of retinoic acid biosynthesis is critical for zebrafish intestinal development and differentiation
    Lincoln D Nadauld
    Department of Oncological Sciences, University of Utah, Salt Lake City 84112, USA
    J Biol Chem 279:51581-9. 2004
    ..colon adenomas and carcinomas lack retinol dehydrogenases (RDHs) and that APC regulates the expression of human RDHL. These data suggest a model wherein APC controls enterocyte differentiation by controlling retinoic acid production...
  58. ncbi Discrete analysis of bile acid in serum and bile with 3 alpha-hydroxysteroid dehydrogenase and diaphorase immobilized onto alkylamine glass beads
    Kirti Rani
    Biochemistry Research Laboratory, Department of Bio Sciences, M D University, Rohtak 124001, Haryana
    Indian J Biochem Biophys 43:98-104. 2006
    ..The cost of bile acid determination for 100 the serum and bile samples by the present method was found to be lower than by a commercially available method (Sigma kit 450-A)...
  59. ncbi Transcript profiling of the androgen signal in normal prostate, benign prostatic hyperplasia, and prostate cancer
    David R Bauman
    Department of Pharmacology, Center of Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, 130C John Morgan Building, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104 6084, USA
    Endocrinology 147:5806-16. 2006
    ..280 in normal PSC) were maintained in PEC and PSC in diseased prostate. These data suggest that CaP may be more responsive to an ERbeta agonist and BPH may be more responsive to androgen ablation...
  60. pmc Characterization of brain neurons that express enzymes mediating neurosteroid biosynthesis
    Roberto C Agis-Balboa
    Department of Psychiatry, Psychiatric Institute, University of Illinois, 1601 Taylor Street, Chicago, IL 60612, USA
    Proc Natl Acad Sci U S A 103:14602-7. 2006
    ....
  61. ncbi Steroid metabolism and profile of steroidogenic gene expression in Episkin: high similarity with human epidermis
    Van Luu-The
    Laboratory of Molecular Endocrinology and Oncology, Laval University Hospital Research Center CRCHUL and Laval University, 2705 Laurier Boulevard, Quebec G1V 4G2, Canada
    J Steroid Biochem Mol Biol 107:30-6. 2007
    ..Except for UGT-glucuronosyl transferase, similar mRNA expression profiles between Episkin and human epidermis are observed...
  62. ncbi Characterization of rat and mouse NAD+-dependent 3alpha/17beta/20alpha-hydroxysteroid dehydrogenases and identification of substrate specificity determinants by site-directed mutagenesis
    Satoshi Endo
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora Higashi, Gifu 502 8585, Japan
    Arch Biochem Biophys 467:76-86. 2007
    ..The replacement of Asp128, Phe129 and Ser137 of AKR1C16 with the corresponding residues (Glu, Ser and Phe, respectively) of AKR1C24 increased the catalytic efficiency for 17beta- and 20alpha-hydroxysteroids...
  63. pmc An indomethacin analogue, N-(4-chlorobenzoyl)-melatonin, is a selective inhibitor of aldo-keto reductase 1C3 (type 2 3alpha-HSD, type 5 17beta-HSD, and prostaglandin F synthase), a potential target for the treatment of hormone dependent and hormone indepe
    Michael C Byrns
    Center for Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, PA 19104 6084, United States
    Biochem Pharmacol 75:484-93. 2008
    ..The identification of CBM as a specific inhibitor of AKR1C3 will aid the investigation of its roles in steroid hormone and prostaglandin signaling and the resultant effects on cancer development...
  64. ncbi Electronic effects of para-substitution on acetophenones in the reaction of rat liver 3alpha-hydroxysteroid dehydrogenase
    Koji Uwai
    Department of Pharmaceutics, Tohoku Pharmaceutical University, 4 4 1 Komatsushima, Sendai 981 8558, Japan
    Bioorg Med Chem 16:1084-9. 2008
    ....
  65. doi [Abnormal expression of genes that regulate retinoid metabolism and signaling in non-small-cell lung cancer]
    E S Kuznetsova
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia
    Mol Biol (Mosk) 50:255-65. 2016
    ..Even at early stages of malignant transformation, a significant decrease in ADH1B, ADH3, RDHL, and RALDH1 mRNA levels was observed in 82, 79, 73, and 64% of tumor specimens, respectively, and a considerable ..
  66. ncbi Role of human type 3 3alpha-hydroxysteroid dehydrogenase (AKR1C2) in androgen metabolism of prostate cancer cells
    Tea Lanisnik Rizner
    Department of Pharmacology, University of Pennsylvania, School of Medicine, 3620 Hamilton Walk, Philadelphia, PA 19104 6084, USA
    Chem Biol Interact 143:401-9. 2003
    ..Based on these findings AKR1C2 may diminish 5alpha-DHT and prevent this ligand from activating the androgen receptor in situ...
  67. ncbi Transient-phase kinetic studies on the nucleotide binding to 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 using fluorescence stopped-flow procedures
    Shigeru Ueda
    Department Diagnostics Research and Development, Division of Fine Chemicals and Diagnostics, Asahi Kasei Pharma Corporation, Shizuoka, Japan
    Eur J Biochem 271:1774-80. 2004
    ..These results suggest that a certain type of nucleotide can modulate nucleotide-binding mode and further the catalytic function of the enzyme...
  68. ncbi Tibolone metabolism in human liver is catalyzed by 3alpha/3beta-hydroxysteroid dehydrogenase activities of the four isoforms of the aldo-keto reductase (AKR)1C subfamily
    Stephan Steckelbroeck
    Department of Pharmacology, University of Pennsylvania School of Medicine, 130C John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104 6084, USA
    J Pharmacol Exp Ther 316:1300-9. 2006
    ..The low hepatic formation of 3alpha-hydroxytibolone suggests that AKR1C4 is not the primary source of this metabolite and instead it maybe formed by an intestinal or enterobacterial 3alpha-HSD...
  69. ncbi Comigration of two autophagosome-associated dehydrogenases on two-dimensional polyacrylamide gels
    Marianne Lunde Sneve
    Proteomics Section, Department of Cell Biology, Institute for Cancer Research, Rikshospitalet Radiumhospitalet HF, University of Oslo, Oslo, Norway
    Autophagy 1:157-62. 2005
    ..Proteomic analysis by a combination of immunological and mass spectrometric identification methods was thus capable of resolving two comigrating dehydrogenases selectively associated with autophagic organelles...
  70. ncbi Androgen inactivation and steroid-converting enzyme expression in abdominal adipose tissue in men
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Laval University, 2705 Laurier Boulevard T3 67, Quebec, Quebec, Canada G1V 4G2
    J Endocrinol 191:637-49. 2006
    ..Further studies are required to elucidate whether local androgen inactivation in abdominal adipose tissue is involved in the modulation of adipocyte metabolism and regional fat distribution in men...
  71. ncbi Purification, crystallization and preliminary X-ray diffraction results of human 17beta-hydroxysteroid dehydrogenase type 5
    Ming Zhou
    Molecular Endocrinology Laboratory, CHUL Research Center, Laval University, Canada
    Acta Crystallogr D Biol Crystallogr 58:1048-50. 2002
    ..41, b = 77.16, c = 48.67 A, beta = 116.32 degrees. Form II crystals obtained at pH 6.5 diffracted to 2.0 A and belonged to space group P6(3), with unit-cell parameters a = b = 110.58, c = 56.89 A...
  72. ncbi Intestinal and hepatic microsomal metabolism of testosterone and progesterone by a 3 alpha-hydroxysteroid dehydrogenase to the 3 alpha-hydroxy derivatives in the channel catfish, Ictalurus punctatus
    Zhen Lou
    Department of Medicinal Chemistry, College of Pharmacy, University of Florida, P O Box 100485, Gainesville 32610 0485, USA
    J Steroid Biochem Mol Biol 82:413-24. 2002
    ..Thus, 3 alpha-hydroxysteroid dehydrogenase is an important pathway in intestinal microsomes of the channel catfish...
  73. pmc Physiological insights into all-trans-retinoic acid biosynthesis
    Joseph L Napoli
    University of California, Berkeley, CA, USA
    Biochim Biophys Acta 1821:152-67. 2012
    ..and genetic data have identified specific functional outcomes for the retinol dehydrogenases, RDH1, RDH10, and DHRS9, as physiological catalysts of the first step in atRA biosynthesis, and for the retinal dehydrogenases RALDH1, ..
  74. pmc Abnormal expression of genes involved in inflammation, lipid metabolism, and Wnt signaling in the adipose tissue of polycystic ovary syndrome
    Gregorio Chazenbalk
    Department of Obstetrics Gynecology, Cedars Sinai Medical Center, Los Angeles, California 90048, USA
    J Clin Endocrinol Metab 97:E765-70. 2012
    ..Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women...
  75. ncbi Biallelic deletion and loss of expression analysis of genes at FRA2G common fragile site in tumor-derived cell lines
    Zaira M Limongi
    Dipartimento di Genetica e Biologia Molecolare, Universita La Sapienza, P le Aldo Moro 5, 00185 Roma, Italy
    Cancer Genet Cytogenet 161:181-6. 2005
    ..Moreover, we observed loss of expression (LOE) of the DHRS9 gene (alias RDHL), one of the deleted genes in the DAUDI cell line, in MOLT-14 and Raji cell lines derived from ..
  76. ncbi Human hepatic 3 alpha-hydroxysteroid dehydrogenase: possible identity with human hepatic chlordecone reductase
    J M Binstock
    Department of Medicine, New York Medical College, Valhalla 10595
    Biochem Biophys Res Commun 187:760-6. 1992
    ..The results suggest that the natural substrates for chlordecone reductase are 3-keto-5-dihydrosteroids and that these two proteins may be identical...
  77. ncbi [Purification and some properties of 3 alpha-hydroxysteroid dehydrogenase from pig adrenal cytosol]
    S Nakajin
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Yakugaku Zasshi 111:775-82. 1991
    ..These results strongly suggest that 3 alpha-HSD purified from pig adrenal cytosol is a bi-functional enzyme catalyzing 3 alpha- and 20 alpha-HSD activities...
  78. ncbi Cloning and sequencing of the cDNA for rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase
    J E Pawlowski
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    J Biol Chem 266:8820-5. 1991
    ..M., Abrams, W. R., and Pawlowski, J. E. (1991) J. Biol. Chem. 266, 8826-8834]. The sequence data presented suggests that 3 alpha-HSD, prostaglandin F synthase, and aldehyde/aldose reductases are members of a common gene family...
  79. ncbi Metabolism of the contraceptive steroid desogestrel by human liver in vitro
    S Madden
    Department of Pharmacology and Therapeutics, University of Liverpool, England
    J Steroid Biochem 35:281-8. 1990
    ..e. in the conversion of 3 beta-OHDg to 3-ketodesogestrel there is no requirement for isomerization). Kinetic parameters of 3 alpha-HSDH were also determined...
  80. ncbi Characterization of NADP+: 3 beta-hydroxysteroid dehydrogenase from microsomes of rat liver
    T Akao
    Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan
    Biochim Biophys Acta 1046:271-6. 1990
    ..The molecular weight of the enzyme was estimated to be about 43,000 by Sephadex G-200 column chromatography. From these results, it is concluded that the enzyme is a new type of microsomal NADP+:3 beta-hydroxysteroid dehydrogenase...
  81. ncbi Effects of repeated androgen treatments on metabolism and nuclear binding of androgen in the infant murine submandibular gland
    H Katsukawa
    Department of Oral Physiology, Asahi University, School of Dentistry, Gifu, Japan
    Comp Biochem Physiol B 93:187-91. 1989
    ..4. These results suggest that there is parallelism between the androgen responses and amounts of nuclear androgen binding, not androgen responses of 5 alpha-reductase and 3 alpha-HSDase...
  82. ncbi Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme
    K H Kondo
    Department of Clinical Chemistry, Karolinska Institute at Huddinge University Hospital, Sweden
    Eur J Biochem 219:357-63. 1994
    ..The substrate specificity of the human enzyme is considerably narrower than that of the rat enzyme, and the enzyme seems to be more important for bile acid biosynthesis than for metabolism of steroid hormones...
  83. ncbi Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning of multiple cDNAs encoding structurally related proteins in humans
    M Khanna
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA
    J Steroid Biochem Mol Biol 53:41-6. 1995
    ..We are currently investigating the functional relationship between the enzymes encoded by these human cDNAs and 3 alpha-hydroxysteroid dehydrogenase...
  84. pmc Purification of NADPH-dependent dehydroascorbate reductase from rat liver and its identification with 3 alpha-hydroxysteroid dehydrogenase
    B Del Bello
    Instituto di Patologia Generale, Universita di Siena, Italy
    Biochem J 304:385-90. 1994
    ..Internal primary structure data were obtained from CNBr-derived fragments and definitely proved the identity of NADPH-dependent DHAA reductase with 3 alpha-hydroxysteroid dehydrogenase...
  85. ncbi Secosteroid mechanism-based inactivators and site-directed mutagenesis as probes for steroid hormone recognition by 3 alpha-hydroxysteroid dehydrogenase
    B P Schlegel
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Biochemistry 33:10367-74. 1994
    ..In the presence and absence of NAD+, 2 and 4 inactivated > 50% of the enzyme in 10 s or less.(ABSTRACT TRUNCATED AT 250 WORDS)..
  86. pmc Three-dimensional structure of rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily
    S S Hoog
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Proc Natl Acad Sci U S A 91:2517-21. 1994
    ..In the 3 alpha-HSD structure these residues are on the periphery of the barrel and are unlikely to participate in catalysis...
  87. pmc Molecular cloning of two human liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with chlordecone reductase and bile-acid binder
    Y Deyashiki
    Biochemistry Laboratory, Gifu Pharmaceutical University, Japan
    Biochem J 299:545-52. 1994
    ....
  88. pmc Molecular cloning of cDNA for rat ovarian 20 alpha-hydroxysteroid dehydrogenase (HSD1)
    R Miura
    Laboratory of Cellular Biochemistry, University of Tokyo, Japan
    Biochem J 299:561-7. 1994
    ....
  89. ncbi Identification of a major prolactin-regulated protein as 20 alpha-hydroxysteroid dehydrogenase: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression
    C T Albarracin
    Department of Physiology and Biophysics, University of Illinois College of Medicine, Chicago 60612
    Endocrinology 134:2453-60. 1994
    ....
  90. ncbi Molecular cloning of multiple cDNAs encoding human enzymes structurally related to 3 alpha-hydroxysteroid dehydrogenase
    K N Qin
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021
    J Steroid Biochem Mol Biol 46:673-9. 1993
    ..Genomic blot analysis using HAKRb as the probe detected multiple DNA fragments hybridized to the probe and a high degree of restriction fragment length polymorphism, suggesting the complexity of this supergene family...
  91. ncbi Steroid metabolizing enzymes in pluripotential progenitor central nervous system cells: effect of differentiation and maturation
    R C Melcangi
    Department of Endocrinology, University of Milan, Italy
    Neuroscience 72:467-75. 1996
    ..These results show that undifferentiated central nervous system cells possess androgen and progestagen metabolizing enzymes which are strongly influenced by the cellular differentiation/maturation process...
  92. ncbi Molecular cloning of human type 3 3 alpha-hydroxysteroid dehydrogenase that differs from 20 alpha-hydroxysteroid dehydrogenase by seven amino acids
    I Dufort
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada
    Biochem Biophys Res Commun 228:474-9. 1996
    ..1, 78.3, and 67.4% identity with rat 3 alpha-HSD and rabbit and rat 20 alpha-HSD, respectively. 3 alpha-HSD3 belongs to the aldo-keto reductase family and like almost all the members of this family preferred NADPH as cofactor...
  93. ncbi Molecular determinants of steroid recognition and catalysis in aldo-keto reductases. Lessons from 3alpha-hydroxysteroid dehydrogenase
    T M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084, USA
    J Steroid Biochem Mol Biol 69:211-25. 1999
    ..Exploitation of these findings using steroidal and nonsteroidal mechanism based inactivators may lead to selective and broad based AKR inhibitors...
  94. pmc Conversion of mammalian 3alpha-hydroxysteroid dehydrogenase to 20alpha-hydroxysteroid dehydrogenase using loop chimeras: changing specificity from androgens to progestins
    H Ma
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    Proc Natl Acad Sci U S A 96:11161-6. 1999
    ..This study represents an example where sex hormone specificity can be changed at the enzyme level...
  95. ncbi Function of human brain short chain L-3-hydroxyacyl coenzyme A dehydrogenase in androgen metabolism
    X Y He
    Department of Pharmacology, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314, USA
    Biochim Biophys Acta 1484:267-77. 2000
    ....
  96. ncbi Functional expression, purification, and characterization of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    E Maser
    Department of Pharmacology and Toxicology, School of Medicine, Philipps University of Marburg, Germany
    Biochem Biophys Res Commun 272:622-8. 2000
    ..testosteroni on the protein level. Upon gel permeation chromatography the purified enzyme elutes as a 49.4 kDa protein revealing for the first time the dimeric nature of 3alpha-HSD/CR of C. testosteroni...
  97. pmc Human 3alpha-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones
    T M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    Biochem J 351:67-77. 2000
    ..These studies are in stark contrast with those in rat where only a single AKR with positional- and stereo-specificity for 3alpha-hydroxysteroids exists...
  98. ncbi Characterization of a human 20alpha-hydroxysteroid dehydrogenase
    Y Zhang
    Oncology and Molecular Endocrinology Research Center, CHUQ PCHUL and Laval University, Quebec, Quebec G1V 4G2, Canada
    J Mol Endocrinol 25:221-8. 2000
    ..The present study clearly indicates that the genuine human 20alpha-HSD belongs to the aldoketo reductase family, like the 20alpha-HSDs from other species...
  99. ncbi Characterization of the 5alpha-reductase-3alpha-hydroxysteroid dehydrogenase complex in the human brain
    S Steckelbroeck
    Department of Clinical Biochemistry, University of Bonn, 53105 Bonn, Germany
    J Clin Endocrinol Metab 86:1324-31. 2001
    ..g. the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids, rather than control of reproductive functions...
  100. ncbi Characterization of a novel type of human microsomal 3alpha -hydroxysteroid dehydrogenase: unique tissue distribution and catalytic properties
    S V Chetyrkin
    Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, 64110, USA
    J Biol Chem 276:22278-86. 2001
    ..Thus, the new short chain dehydrogenase represents a novel type of microsomal NAD(+)-dependent 3alpha-hydroxysteroid dehydrogenase with unique catalytic properties and tissue distribution...
  101. ncbi Characterization of a novel airway epithelial cell-specific short chain alcohol dehydrogenase/reductase gene whose expression is up-regulated by retinoids and is involved in the metabolism of retinol
    C M Soref
    Center for Comparative Respiratory Biology and Medicine and the Department of Nutrition, University of California at Davis, 95616, USA
    J Biol Chem 276:24194-202. 2001
    ..The results suggested that the novel gene represents a new member of the SDR family that may play a critical role in retinol metabolism in airway epithelia as well as in other epithelia of colon, tongue, and esophagus...

Research Grants16

  1. INHIBITION OF 3A HSD BY ANTI-INFLAMMATORY DRUGS
    TREVOR PENNING; Fiscal Year: 1991
    ..These studies may provide information for a rational approach to the design of superior antiinflammatory drugs...
  2. Symposium on Aldo-Keto Reductases & Toxicant Metabolism
    TREVOR PENNING; Fiscal Year: 2002
    ..abstract_text> ..
  3. Molecular Mechanisms of Multistage Carcinogenesis
    TREVOR PENNING; Fiscal Year: 2006
    ..By studying the discrete molecular events responsible for the causation of cancer this Program may lead to the early prevention and intervention of this disease. ..
  4. Northeast Under/Grad Research Organization for Neuroscience
    Cheryl Frye; Fiscal Year: 2008
    ..Both of these outcomes are congruous with the Public Health Service's mission to ensure that there is a resource of well-trained scientists and health-care providers in the United States. [unreadable] [unreadable] [unreadable]..
  5. PHARMACOLOGY--ALLOSTERIC MODULATORS OF GABAA RECEPTORS
    Alessandro Guidotti; Fiscal Year: 2002
    ....
  6. REELIN DEFICIENCY--MODEL FOR SCHIZOPHRENIA VULNERABILITY
    Alessandro Guidotti; Fiscal Year: 2004
    ....
  7. REGULATION OF REPRODUCTION
    Synthia Mellon; Fiscal Year: 2005
    ..abstract_text> ..
  8. PATHOLOGY OF THE LABORATORY MOUSE
    John Sundberg; Fiscal Year: 2006
    ..The Workshop will accommodate 16 participants who will have an opportunity to interact with a group of prominent mouse pathologists and geneticists from both TJL, and other leading institutions. ..
  9. RETINOID METABOLISM
    JOSEPH NAPOLI; Fiscal Year: 2007
    ..This work will contribute to revealing pathology caused or exacerbated by impaired atRA biosynthesis during carcinogenesis, skin disease, birth defects, or diseases of intermediary metabolism. ..
  10. Biosynthesis of 9-cis-retinoic acid/retinol homeostasis
    JOSEPH NAPOLI; Fiscal Year: 2007
    ..This work will provide new insight into retinoid metabolism/ function. ..
  11. Alopecia and Ulcerative Dermatitis in B6 Mice
    John Sundberg; Fiscal Year: 2007
    ..Dehydrogenase/reductase SDR family member 9 (Dhrs9) protein levels are upregulated in B6 substrains with high alopecia frequency further producting retinoic acid...
  12. Xenogen Rodent Imaging System
    JOSEPH NAPOLI; Fiscal Year: 2008
    ..Availability of this instrument would undoubtedly stimulate its use by others on campus and in the area. [unreadable] [unreadable] [unreadable]..
  13. RESEARCH TRAINING FOR VETERINARIANS: ANIMAL MODELS
    John Sundberg; Fiscal Year: 2008
    ..The investigators ask to maintain the three slots currently funded. This program is designed to meet the increasing demand for scientists in functional genomics research. ..
  14. Mouse models for GABA epigenetic dysfunction
    Alessandro Guidotti; Fiscal Year: 2009
    ..abstract_text> ..
  15. RETINAL DEHYDROGENASES
    JOSEPH NAPOLI; Fiscal Year: 2009
    ..The fundamental insight and reagents generated will be useful to identify diseases and age-related degeneration that may be caused or exacerbated by impaired RA biosynthesis. ..