DGKE

Summary

Gene Symbol: DGKE
Description: diacylglycerol kinase, epsilon 64kDa
Alias: DAGK5, DAGK6, DGK, NPHS7, DAG kinase epsilon, DGK-epsilon, diacylglycerol kinase epsilon, diglyceride kinase epsilon
Species: human

Top Publications

  1. ncbi The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain
    F Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    Biochem J 318:583-90. 1996
  2. ncbi Phospholipase C/diacylglycerol kinase-mediated signalling is required for benzothiadiazole-induced oxidative burst and hypersensitive cell death in rice suspension-cultured cells
    Jie Chen
    Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang
    Protoplasma 230:13-21. 2007
  3. ncbi Phosphatidic acid regulates signal output by G protein coupled receptors through direct interaction with phospholipase C-beta(1)
    Irene Litosch
    Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, Florida 33101, USA
    Cell Signal 21:1379-84. 2009
  4. ncbi Cloning and characterization of diacylglycerol kinase iota splice variants in rat brain
    Tsukasa Ito
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Japan
    J Biol Chem 279:23317-26. 2004
  5. ncbi The pattern of deoxycytidine- and deoxyguanosine kinase activity in relation to messenger RNA expression in blood cells from untreated patients with B-cell chronic lymphocytic leukemia
    Kourosh Lotfi
    Department of Medicine and Care, Clinical Pharmacology, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    Biochem Pharmacol 71:882-90. 2006
  6. ncbi EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes
    K Yamada
    Department of Liberal Arts and Sciences, School of Health Sciences, Sapporo Medical University, Japan
    Biochem J 321:59-64. 1997
  7. ncbi The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase
    Elizabeth H Clark
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biochemistry 42:11065-73. 2003
  8. ncbi Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo
    Antony M Jose
    Department of Molecular, Cellular, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 280:2730-6. 2005
  9. ncbi Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats
    I Masai
    Molecular Genetics Research Laboratory, Faculty of Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 90:11157-61. 1993
  10. ncbi Regulation of neurite outgrowth in N1E-115 cells through PDZ-mediated recruitment of diacylglycerol kinase zeta
    Yury Yakubchyk
    Department of Cellular and Molecular Medicine, University of Ottawa, Ontario, Canada
    Mol Cell Biol 25:7289-302. 2005

Research Grants

  1. ENZYMOLOGY OF GOLGI STACK FORMATION
    Vivek Malhotra; Fiscal Year: 2007
  2. Molecular Dissection of T Cell Anergy
    Thomas Gajewski; Fiscal Year: 2005
  3. DGK in T Cell Regulation and Tolerance
    Thomas F Gajewski; Fiscal Year: 2010
  4. DISSECTING G PROTEIN PATHWAYS
    Joshua Kaplan; Fiscal Year: 2000
  5. Regulatory Pathways in Osteoclasts and Immune Cells during Inflammatory Arthritis
    Roberta Faccio; Fiscal Year: 2010
  6. Mechanistic Analysis of T Cell Polarity by Photoactivation of Single Cells
    MORGAN A HUSE; Fiscal Year: 2010
  7. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2005
  8. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2007
  9. Vitamin D Hormone: Function and Mechanism of Action
    Sylvia Christakos; Fiscal Year: 2007
  10. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009

Scientific Experts

Detail Information

Publications118 found, 100 shown here

  1. ncbi The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain
    F Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    Biochem J 318:583-90. 1996
    All mammalian diacylglycerol kinase (DGK) isoenzymes so far cloned consist of four conserved regions, namely, C1, C2 (tandem EF-hand structures), C3 (tandem cysteine-rich zinc finger sequences) and the C-terminal C4 domains...
  2. ncbi Phospholipase C/diacylglycerol kinase-mediated signalling is required for benzothiadiazole-induced oxidative burst and hypersensitive cell death in rice suspension-cultured cells
    Jie Chen
    Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang
    Protoplasma 230:13-21. 2007
    The involvement of phospholipase C/diacylglycerol kinase (PLC/DGK)-mediated signalling in oxidative burst and hypersensitive cell death was studied in rice suspension-cultured cells treated with benzothiadiazole (BTH) and infected by ..
  3. ncbi Phosphatidic acid regulates signal output by G protein coupled receptors through direct interaction with phospholipase C-beta(1)
    Irene Litosch
    Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, Florida 33101, USA
    Cell Signal 21:1379-84. 2009
    ..generated downstream of monomeric Rho GTPases via phospholipase D (PLD) and additionally by diacylglycerol kinases (DGK), both stimulates phospholipase C-beta(1) (PLC-beta(1)) and potentiates stimulation of PLC-beta(1) activity by ..
  4. ncbi Cloning and characterization of diacylglycerol kinase iota splice variants in rat brain
    Tsukasa Ito
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Japan
    J Biol Chem 279:23317-26. 2004
    Diacylglycerol kinase (DGK) catalyzes phosphorylation of a second messenger diacylglycerol (DG) to phosphatidic acid in cellular signal transduction...
  5. ncbi The pattern of deoxycytidine- and deoxyguanosine kinase activity in relation to messenger RNA expression in blood cells from untreated patients with B-cell chronic lymphocytic leukemia
    Kourosh Lotfi
    Department of Medicine and Care, Clinical Pharmacology, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    Biochem Pharmacol 71:882-90. 2006
    Deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK) catalyze the first step in the intracellular cascade of fludarabine (2-fluoroadenine-beta-D-arabinofuranoside) and cladribine (2-chlorodeoxyadenosine) phosphorylation, which ..
  6. ncbi EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes
    K Yamada
    Department of Liberal Arts and Sciences, School of Health Sciences, Sapporo Medical University, Japan
    Biochem J 321:59-64. 1997
    The three diacylglycerol kinase isoenzymes (DGK alpha, DGK beta and DGK gamma) cloned so far contain in common a tandem repeat of EF-hand motifs...
  7. ncbi The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase
    Elizabeth H Clark
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biochemistry 42:11065-73. 2003
    ..We have studied the effect of mutating the five Trp residues in the diacylglycerol kinase (DGK) of Escherichia coli to Leu residues...
  8. ncbi Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo
    Antony M Jose
    Department of Molecular, Cellular, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 280:2730-6. 2005
    Diacylglycerol kinases (DGKs) inhibit diacylglycerol (DAG) signaling by phosphorylating DAG. DGK-1, the Caenorhabditis elegans ortholog of human neuronal DGK, inhibits neurotransmission to control behavior...
  9. ncbi Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats
    I Masai
    Molecular Genetics Research Laboratory, Faculty of Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 90:11157-61. 1993
    ..Biochemically, the rdgA mutant lacks an eye-specific and membrane-associated diacylglycerol kinase (DGK; EC 2.7.1.107) activity in a gene-dosage-dependent manner, suggesting that rdgA gene encodes a DGK...
  10. ncbi Regulation of neurite outgrowth in N1E-115 cells through PDZ-mediated recruitment of diacylglycerol kinase zeta
    Yury Yakubchyk
    Department of Cellular and Molecular Medicine, University of Ottawa, Ontario, Canada
    Mol Cell Biol 25:7289-302. 2005
    Syntrophins are scaffold proteins that regulate the subcellular localization of diacylglycerol kinase zeta (DGK-zeta), an enzyme that phosphorylates the lipid second-messenger diacylglycerol to yield phosphatidic acid...
  11. ncbi Diacylglycerol kinase delta associates with receptor for activated C kinase 1, RACK1
    Shin ichi Imai
    Department of Biochemistry, Sapporo Medical University School of Medicine, South 1, West 17, Chuo Ku, Sapporo, 060 8556, Japan
    Biochim Biophys Acta 1791:246-53. 2009
    The delta-isozyme (type II) of diacylglycerol kinase (DGK) is known to positively regulate growth factor receptor signaling...
  12. ncbi Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway
    Ana Ramos Díaz
    Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología Molecular de Plantas, Calle 43, 130 Col Chuburná de Hidalgo, Merida Yucatan, Mexico
    Planta 225:393-401. 2007
    ..1.4.4) or via Phospholipase C (PLC; EC: 3.1.4.3), and diacylglycerol kinase (DGK; EC 2.7.1.107)...
  13. ncbi Molecular species of phosphatidylinositol-cycle intermediates in the endoplasmic reticulum and plasma membrane
    Yulia V Shulga
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 49:312-7. 2010
    ..by comparing the compositions of the two membranes isolated from embryonic fibroblasts obtained from diacylglycerol kinase epsilon (DGKepsilon) knockout (KO) and wild-type (WT) mice...
  14. ncbi The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding
    S Ahmed
    Institute of Molecular and Cell Biology, National University of Singapore
    Biochem J 280:233-41. 1991
    ..DG is produced transiently as it is rapidly metabolized by DG kinase (DGK) to phosphatidic acid...
  15. ncbi A 104-kDa diacylglycerol kinase containing ankyrin-like repeats localizes in the cell nucleus
    K Goto
    Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan
    Proc Natl Acad Sci U S A 93:11196-201. 1996
    ..This cDNA encoded a 929-aa, 104-kDa polypeptide termed DGK-IV...
  16. ncbi Diacylglycerol kinase beta accumulates on the perisynaptic site of medium spiny neurons in the striatum
    Yasukazu Hozumi
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Eur J Neurosci 28:2409-22. 2008
    ..The former activates protein kinase C and the latter mobilizes Ca(2+) from intracellular store. DAG kinase (DGK) then phosphorylates DAG to produce another second messenger (phosphatidic acid)...
  17. ncbi Nod factor-induced phosphatidic acid and diacylglycerol pyrophosphate formation: a role for phospholipase C and D in root hair deformation
    M den Hartog
    Swammerdam Institute for Life Sciences, Department of Plant Physiology, University of Amsterdam, Kruislaan 318, NL 1098 SM, Amsterdam, The Netherlands
    Plant J 25:55-65. 2001
    ..PA) and diacylglycerol pyrophosphate, indicative of PLD or PLC activity in combination with diacylglycerol kinase (DGK) and PA kinase. Treatment with Nod factor had similar effects, although less pronounced...
  18. ncbi Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26
    G T Ma
    Department of Biochemistry, Ohio State University, Columbus 43210 1292
    J Biol Chem 270:6595-601. 1995
    ..kinases, deoxycytidine kinase/deoxyadenosine kinase (dCK/dAK) and deoxyguanosine kinase/deoxyadenosine kinase (dGK/dAK) are required, together with thymidine kinase (TK), for deoxynucleotide synthesis in Lactobacillus acidophilus ..
  19. ncbi Low-intensity exercise increases skeletal muscle protein expression of PPARdelta and UCP3 in type 2 diabetic patients
    Tomas Fritz
    Centre of Family Medicine, Karolinska University Hospital, Karolinska Institutet, 171 11 Stockholm, Sweden
    Diabetes Metab Res Rev 22:492-8. 2006
    ..We tested the hypothesis that changes in expression of key genes in skeletal muscles relate to exercise-induced improvements in type 2 diabetic patients...
  20. ncbi [RasGRP proteins--Ras-activating factors]
    Mateusz Szamałek
    Katedra Biochemii Farmaceutycznej, Uniwersytet Medyczny im K Marcinkowskiego, Poznan
    Postepy Biochem 53:112-20. 2007
    ..signal transmitted by RasGRP is terminated as a result of DAG phosphorylation catalyzed by diacylglycerol kinase (DGK)...
  21. ncbi Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity
    Martin Welin
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Box 590, Biomedical Center, S 751 24 Uppsala, Sweden
    J Mol Biol 366:1615-23. 2007
    ..Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known...
  22. ncbi Protein kinase C alpha phosphorylates and negatively regulates diacylglycerol kinase zeta
    Bai Luo
    Huntsman Cancer Institute, and Department of Oncological Sciences, University of Utah, Salt Lake City, Utah 84112, USA
    J Biol Chem 278:39542-7. 2003
    Diacylglycerol kinase (DGK) terminates diacylglycerol (DAG) signaling by phosphorylating DAG to produce phosphatidic acid, which also has signaling properties...
  23. ncbi Nuclear localization of diacylglycerol kinase zeta in neurons
    Yasukazu Hozumi
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Eur J Neurosci 18:1448-57. 2003
    Diacylglycerol kinase (DGK) is involved in intracellular signal transduction as a regulator of levels of diacylglycerol which leads to protein kinase C activation...
  24. ncbi Localization of diacylglycerol kinase epsilon on stress fibers in vascular smooth muscle cells
    Tomoyuki Nakano
    Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Mukogawa cho 1 1, Nishinomiya, Hyogo, 663 8501, Japan
    Cell Tissue Res 337:167-75. 2009
    The expression pattern of diacylglycerol kinase (DGK) and the biological significance of DGKepsilon in vascular smooth muscle cells were investigated...
  25. ncbi Kinase activity of the dgk gene product is involved in the virulence of Streptococcus mutans
    Yukie Shibata
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Microbiology 155:557-65. 2009
    C-terminal deletion of the diacylglycerol kinase (Dgk) homologue of the cariogenic oral bacterium Streptococcus mutans resulted in loss of aciduricity...
  26. ncbi Morphological changes and spatial regulation of diacylglycerol kinase-zeta, syntrophins, and Rac1 during myoblast fusion
    Hanan Abramovici
    Department of Cellular and Molecular Medicine, Center for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada
    Cell Motil Cytoskeleton 64:549-67. 2007
    ..Our recent studies suggest diacylglycerol kinase-zeta (DGK-zeta), an enzyme that metabolizes diacylglycerol to yield phosphatidic acid, plays an important role in actin ..
  27. ncbi Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin-binding isoform
    W A Snedden
    Department of Botany, University of Toronto, Toronto, Ontario, Canada, M5S 3B2
    Plant J 24:317-26. 2000
    ..Both recombinant LeCBDGK and LeDGK1 were catalytically active in vitro. Anti-DGK antiserum detected two immunoreactive proteins associated with microsomal and plasma membrane fractions from cell ..
  28. ncbi Measurement of mammalian diacylglycerol kinase activity in vitro and in cells
    Richard M Epand
    Department of Biochemistry and Biomedical Sciences, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada
    Methods Enzymol 434:293-304. 2007
    Diacylglycerol kinase (DGK) catalyzes the conversion of diacylglycerol to phosphatidic acid. Because both the lipid substrate and the product are important in regulation, this enzyme plays an important role in signal transduction...
  29. ncbi Determination of the topology of the hydrophobic segment of mammalian diacylglycerol kinase epsilon in a cell membrane and its relationship to predictions from modeling
    Marc Decaffmeyer
    Faculte Universitaire des Sciences Agronomiques de Gembloux, Centre de Biophysique Moleculaire Numerique, Passage des Deportes, 2, 5030 Gembloux, Belgium
    J Mol Biol 383:797-809. 2008
    ..The work also demonstrates that the wild-type DGKepsilon is a monotopic protein...
  30. ncbi Dramatic differences in the roles in lipid metabolism of two isoforms of diacylglycerol kinase
    Stephen B Milne
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Biochemistry 47:9372-9. 2008
    ....
  31. ncbi AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression
    Fernando C Gómez-Merino
    University of Potsdam, Institute of Biochemistry and Biology, Karl Liebknecht Strasse 24 25, Haus 20, D 14476 Golm Potsdam, Germany
    J Biol Chem 279:8230-41. 2004
    Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Both DAG and PA are implicated in signal transduction pathways...
  32. ncbi Cardiac-specific overexpression of diacylglycerol kinase zeta attenuates left ventricular remodeling and improves survival after myocardial infarction
    Takeshi Niizeki
    Department of Cardiology, Yamagata University School of Medicine, 2 2 2 Iida nishi, Yamagata, Japan 990 9585
    Am J Physiol Heart Circ Physiol 292:H1105-12. 2007
    ..DAG kinase (DGK) phosphorylates DAG and controls cellular DAG levels, thus acting as a negative regulator of PKC and subsequent ..
  33. ncbi A traditional herbal medicine, rikkunshi-to (TJ-43), prevents intracellular signaling disorders in gastric smooth muscle of diabetic rats
    Yasushi Sakai
    Division of Physiology, Department of Occupational Therapy, School of Nursing and Rehabilitation Sciences, Showa University, Yokohama 226, Japan
    Am J Chin Med 32:245-56. 2004
    ..The present study demonstrated that diacylglycerol kinase (DGK) activity in diabetic gastric smooth muscle in the resting state was approximately 3...
  34. ncbi Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism
    M K Topham
    The Huntsman Cancer Institute and Department of Internal Medicine, University of Utah, Salt Lake City, Utah 84112, USA
    J Cell Biol 152:1135-43. 2001
    ..b>DGK zeta, but not other DGKs, completely eliminated Ras activation induced by RasGRP, and DGK activity was required for ..
  35. ncbi Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy
    Hirokazu Yahagi
    Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai 980 8574, Japan
    Biochem Biophys Res Commun 332:101-8. 2005
    To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation...
  36. ncbi Role of diacylglycerol kinase alpha in the attenuation of receptor signaling
    M A Sanjuan
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    J Cell Biol 153:207-20. 2001
    Diacylglycerol kinase (DGK) is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid...
  37. ncbi Pharmacological evidence for the involvement of diacylglycerol lipase in depolarization-induced endocanabinoid release
    Yuki Hashimotodani
    Department of Neurophysiology, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Neuropharmacology 54:58-67. 2008
    ..The chemicals we tested include inhibitors for phospholipase C (U73122 and ET-18), diacylglycerol kinase (DGK inhibitor 1), phosphatidic acid phosphohydrolase (propranolol), and diacylglycerol lipase (DGL; RHC-80267 and ..
  38. ncbi Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides
    L Wang
    Department of Veterinary Medical Chemistry, The Swedish University of Agricultural Sciences, The Biomedical Centre, SE 751 23 Uppsala, Sweden
    Mol Microbiol 42:1065-73. 2001
    ..High levels of thymidine kinase (TK), deoxycytidine kinase (dCK), deoxyguanosine kinase (dGK) and deoxyadenosine kinase (dAK) activities were detected in extracts from Mycoplasma pneumoniae, Mycoplasma ..
  39. ncbi The alpha isoform of diacylglycerol kinase exhibits arachidonoyl specificity with alkylacylglycerol
    Richard M Epand
    Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 43:14778-83. 2004
    We compared the diacylglycerol kinase (DGK) catalyzed phosphorylation of 1-O-hexanoyl-2-oleoylglycerol (HOG) with 1-O-hexanoyl-2-arachidonoylglycerol (HAG)...
  40. ncbi Diacylglycerol kinase-theta is localized in the speckle domains of the nucleus
    Giovanna Tabellini
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Sezione di Anatomia Umana, Cell Signalling Laboratory, Universita di Bologna, 40126, Bologna, Italy
    Exp Cell Res 287:143-54. 2003
    ..Here, we show, by immunofluorescence staining and confocal analysis, that DGK-theta localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12, and HeLa...
  41. ncbi Epileptogenesis in diacylglycerol kinase epsilon deficiency up-regulates COX-2 and tyrosine hydroxylase in hippocampus
    Walter J Lukiw
    Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, School of Medicine in New Orleans, LA 70112, USA
    Biochem Biophys Res Commun 338:77-81. 2005
    ..Of the nine mammalian DGK isotypes known, DGK epsilon (DGKepsilon) shows specificity for arachidonoyldiacylglycerol (20:4-DAG) and selectively contributes to ..
  42. ncbi Anabolism of amdoxovir: phosphorylation of dioxolane guanosine and its 5'-phosphates by mammalian phosphotransferases
    Joy Y Feng
    Gilead Sciences, 4 University Place, 4611 University Drive, Durham, NC 27707, USA
    Biochem Pharmacol 68:1879-88. 2004
    ..DXG using 15 purified enzymes from human (8), animal (6), and yeast (1) sources, including deoxyguanosine kinase (dGK), deoxycytidine kinase (dCK), high Km 5'-nucleotidase (5'-NT), guanylate (GMP) kinase, nucleoside monophosphate (..
  43. ncbi Nitric oxide is critical for inducing phosphatidic acid accumulation in xylanase-elicited tomato cells
    Ana M Laxalt
    Instituto de Investigaciones Biologicas, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina
    J Biol Chem 282:21160-8. 2007
    ..second messenger phosphatidic acid (PA) via the activation of the phospholipase C (PLC) and diacylglycerol kinase (DGK) pathway. Defense-related responses downstream of PA were studied...
  44. ncbi Does aluminum generate a bonafide phospholipd signal cascade?
    Ana Ramos-Díaz
    Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán Mérida Yucatán, Mexico
    Plant Signal Behav 2:263-4. 2007
    ..The most important PA formation routes in plant signalling are: phospholipase C (PLC)/diacylglycerol kinase (DGK) and phospholipase D (PLD)...
  45. ncbi Phosphatidic acid plays a regulatory role in clathrin-mediated endocytosis
    Costin N Antonescu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA
    Mol Biol Cell 21:2944-52. 2010
    ..by manipulating the activities and/or levels of either phospholipase D (PLD1 and PLD2) or diacylglycerol kinase (DGK), two enzyme classes involved in PA production...
  46. ncbi HCN pacemaker channel activation is controlled by acidic lipids downstream of diacylglycerol kinase and phospholipase A2
    Keri J Fogle
    Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
    J Neurosci 27:2802-14. 2007
    ..that 4betaPMA exerts its effects on HCN gating via sequential activation of PKC and diacylglycerol kinase (DGK) coupled with upregulation of MAPK (mitogen-activated protein kinase) and phospholipase A2 (PLA2), but its action ..
  47. ncbi Phosphatidic acid signaling regulation of Ras superfamily of small guanosine triphosphatases
    Yueqiang Zhang
    Department of Integrative Biology and Pharmacology University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    Biochim Biophys Acta 1791:850-5. 2009
    ..Recent studies indicate that the signaling PA generated from phospholipase D (PLD) and diacylglycerol kinase (DGK) plays critical roles in regulating the activity of some members of Ras superfamily of small guanosine ..
  48. ncbi Activation of diacylglycerol kinase alpha is required for VEGF-induced angiogenic signaling in vitro
    Gianluca Baldanzi
    Department of Medical Sciences, University Amedeo Avogadro of Piemonte Orientale, v Solaroli 17, 28100, Novara, Italy
    Oncogene 23:4828-38. 2004
    ..As we had previously shown that Src-mediated activation of diacylglycerol kinase-alpha (Dgk-alpha) is required for hepatocytes growth factor-stimulated cell migration, we asked whether Dgk-alpha is involved ..
  49. ncbi Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells
    M Kai
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    J Biol Chem 269:18492-8. 1994
    In order to clone novel diacylglycerol kinase (DGK) isozymes, we first obtained a DGK-related cDNA fragment by polymerase chain reaction using the human hepatoma cell line HepG2 mRNA and degenerated primers...
  50. ncbi Diacylglycerol kinase zeta inhibits myocardial atrophy and restores cardiac dysfunction in streptozotocin-induced diabetes mellitus
    Olga Bilim
    Department of Cardiology, Pulmonology and Nephrology, Yamagata University School of Medicine, Yamagata, Japan
    Cardiovasc Diabetol 7:2. 2008
    ..Diacylglycerol kinase (DGK) converts DAG to phosphatidic acid and acts as an endogenous regulator of PKC activity...
  51. ncbi Determinants of sensitivity of human T-cell leukemia CCRF-CEM cells to immucillin-H
    Min Huang
    Department of Medicine, Stanford University, Stanford, CA 94305, USA
    Leuk Res 32:1268-78. 2008
    ..demonstrated undetectable deoxycytidine kinase (dCK) activity, suggesting that dCK and not deoxyguanosine kinase (dGK) was the rate-limiting enzyme for phosphorylation of dGuo in these cells...
  52. ncbi Bovine viral diarrhea viruses differentially alter the expression of the protein kinases and related proteins affecting the development of infection and anti-viral mechanisms in bovine monocytes
    George V Pinchuk
    Department of Sciences and Mathematics, Mississippi University for Women, 1100 College St, W 100, Columbus, MS 39701, USA
    Biochim Biophys Acta 1784:1234-47. 2008
    ..the expression of the receptor of activated C kinase (RACK), of pyridoxal kinase (PK), diacyglycerol kinase (DGK) and Brutons tyrosine kinase (BTK) was decreased in monocytes infected with cp BVDV compared to ncp BVDV, possibly ..
  53. ncbi Diacylglycerol kinase zeta rescues G alpha q-induced heart failure in transgenic mice
    Takeshi Niizeki
    Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, 2 2 2 Iida nishi, Yamagata 990 9585, Japan
    Circ J 72:309-17. 2008
    ..DAG kinase (DGK) catalyzes DAG and controls its cellular levels, thus acting as a regulator of GPCR signaling...
  54. ncbi Diacylglycerol kinases: at the hub of cell signalling
    Isabel Merida
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, Darwin, 3, UAM Campus Cantoblanco, E 28049 Madrid, Spain
    Biochem J 409:1-18. 2008
    ..The product of the DGK reaction, PA, is also linked to the regulation of diverse functions, including cell growth, membrane trafficking, ..
  55. ncbi Nuclear diacylglycerol kinases: regulation and roles
    Daniel M Raben
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe St, Baltimore, MD 21205, USA
    Front Biosci 13:590-7. 2008
    ..localization, modulation of intrinsic enzymatic activity, and the potential physiological roles of the six diacylglycerol kinases that have been found in the nucleus: DGK-alpha, DGK-gamma, DGK-delta, DGK-zeta, DGK-iota, and DGK-theta.
  56. ncbi Substrate specificity of diacylglycerol kinase-epsilon and the phosphatidylinositol cycle
    Yulia V Shulga
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    FEBS Lett 585:4025-8. 2011
    We show that diacylglycerol kinase-ε (DGKε) has less preference for the acyl chain at the sn-1 position of diacylglycerol (DAG) than the one at the sn-2 position...
  57. ncbi Regulation of clathrin-dependent endocytosis by diacylglycerol kinase delta: importance of kinase activity and binding to AP2alpha
    Takumi Kawasaki
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Biochem J 409:471-9. 2008
    ..These results demonstrate that binding between DGKdelta2 and AP2alpha is involved in the transferrin internalization and that DGK activity is also necessary for the regulation of the endocytic process.
  58. ncbi A fluorescence method to define transmembrane alpha-helices in membrane proteins: studies with bacterial diacylglycerol kinase
    Jiraphun Jittikoon
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochemistry 46:10950-9. 2007
    ..Diacylglycerol kinase (DGK) from Escherichia coli contains three transmembrane (TM) alpha-helices per monomer...
  59. ncbi Regulation and roles of neuronal diacylglycerol kinases: a lipid perspective
    Becky Tu-Sekine
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, 21205 MD, USA
    Crit Rev Biochem Mol Biol 46:353-64. 2011
    ..studies have identified eight of these isoforms in various regions of the mammalian central nervous system (CNS): DGK-α, DGK-β, DGK-γ, DGK-η, DGK-ζ, DGK-ι, DGK-ϵ, and DGK-θ...
  60. ncbi Molecular mechanisms of mitochondrial DNA depletion diseases caused by deficiencies in enzymes in purine and pyrimidine metabolism
    Staffan Eriksson
    Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Nucleosides Nucleotides Nucleic Acids 27:800-8. 2008
    ..metabolism give MDS, for example, the mitochondrial thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK) genes. Sixteen TK2 and 22 dGK alterations are known. Their characteristics and symptoms are described...
  61. ncbi Roles for class IIA phosphatidylinositol transfer protein in neurotransmission and behavioral plasticity at the sensory neuron synapses of Caenorhabditis elegans
    Ryo Iwata
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 108:7589-94. 2011
    ..in pitp-1 mutants by expression of the phosphatidylinositol transfer domain alone, and also by mutations of a DGK gene that cause accumulation of DAG, suggesting that PITP-1 serves for DAG production via phosphatidylinositol ..
  62. ncbi Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta
    Camilla Evangelisti
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Cell Signaling Laboratory, Universita di Bologna, Bologna, Italy
    Cell Cycle 9:384-8. 2010
    Diacylglycerol kinases (DGKs) are key regulators of diacylglycerol-dependent signaling pathways. Among the 10 DGK isoforms, DGK-zeta is the only nuclear form that contains a nuclear localization signal...
  63. ncbi Deoxynucleoside salvage enzymes and tissue specific mitochondrial DNA depletion
    L Wang
    Department of Anatomy, Physiology and Biochemistry, Section of Veterinary Medical Biochemistry, Uppsala, Sweden
    Nucleosides Nucleotides Nucleic Acids 29:370-81. 2010
    ..The question why MDS caused by TK2 and p53R2 mutations are predominantly muscle specific while dGK deficiency affected mainly liver will be addressed.
  64. ncbi Phospholipid signaling responses in salt-stressed rice leaves
    Essam Darwish
    Section of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
    Plant Cell Physiol 50:986-97. 2009
    ..PA signaling can be generated via the combined action of phospholipase C (PLC) and diacylglycerol kinase (DGK) or directly via phospholipase D (PLD). The latter can be measured in vivo, using a transphosphatidylation assay...
  65. ncbi Diacylglycerol kinase eta augments C-Raf activity and B-Raf/C-Raf heterodimerization
    Satoshi Yasuda
    Department of Biochemistry, Sapporo Medical University School of Medicine, South 1, West 17, Chuo Ku, Sapporo 060 8556
    J Biol Chem 284:29559-70. 2009
    ..Conversely, the overexpression of DGKeta1 could activate the Ras/B-Raf/C-Raf/MEK/ERK pathway in a DGK activity-independent manner, suggesting that DGKeta serves as a scaffold/adaptor protein...
  66. ncbi Altered gene transcription profiles in fibroblasts harboring either TK2 or DGUOK mutations indicate compensatory mechanisms
    Joan Villarroya
    Centre d Investigacions en Bioquimica i Biologia Molecular CIBBIM, Hospital Universitari Vall d Hebron, Institut de Recerca, Plta 1 Lab 117, Pg Vall d Hebron, 119 129, 08035 Barcelona, Spain
    Exp Cell Res 315:1429-38. 2009
    ..Real-time PCR revealed mtDNA depletion in dGK-deficient fibroblasts (dGK-) but not in TK2-deficient cells (TK2-)...
  67. ncbi Hepatic expression profiling shows involvement of PKC epsilon, DGK eta, Tnfaip, and Rho kinase in type 2 diabetic nephropathy rats
    Jeena Gupta
    Laboratory of Chromatin Biology, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research NIPER, S A S Nagar, Punjab, India
    J Cell Biochem 111:944-54. 2010
    ..in expression of PKC epsilon, TNF-alpha-induced protein (four- to seven-folds), and decrease in the expression of DGK eta in the liver of diabetic nephropathic rats...
  68. ncbi Zinc binding drives sheet formation by the SAM domain of diacylglycerol kinase δ
    Mary Jane Knight
    Department of Chemistry and Biochemistry, Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095 1570, USA
    Biochemistry 49:9667-76. 2010
    The diacylglycerol kinase (DGK) family of enzymes plays critical roles in lipid signaling pathways by converting diacylglycerol to phosphatidic acid, thereby downregulating signaling by the former and upregulating signaling by the latter ..
  69. ncbi [Pulmonary hypertension due to left heart disease: recommendations of the Cologne Consensus Conference 2010]
    S Rosenkranz
    Klinik III fur Innere Medizin, Herzzentrum der Universität zu Köln, Koln
    Dtsch Med Wochenschr 135:S102-14. 2010
    ..In June 2010, a Consensus Conference organized by the PH working groups of the German Society of Cardiology (DGK), the German Society of Respiratory Medicine (DGP) and the German Society of Pediatric Cardiology (DGPK) was held ..
  70. ncbi [Non-invasive diagnosis of pulmonary hypertension: ESC/ERS Guidelines with commentary of the Cologne Consensus Conference 2010]
    E Grunig
    Zentrum für pulmonale Hypertonie, Thoraxklinik am Universitatsklinikum Heidelberg, Heidelberg
    Dtsch Med Wochenschr 135:S67-77. 2010
    ..In June 2010, a Consensus Conference organized by the PH working groups of the German Society of Cardiology (DGK), the German Society of Respiratory Medicine (DGP) and the German Society of Pediatric Cardiology (DGPK) was held ..
  71. ncbi [Pulmonary hypertension: hemodynamic evaluation: hemodynamic evaluation – recommendations of the Cologne Consensus Conference 2010]
    C F Opitz
    Klinik fur Innere Medizin, Schwerpunkt Kardiologie, DRK Kliniken Berlin Köpenick, Berlin
    Dtsch Med Wochenschr 135:S78-86. 2010
    ..In June 2010, a Consensus Conference organized by the PH working groups of the German Society of Cardiology (DGK), the German Society of Respiratory Medicine (DGP) and the German Society of Paediatric Cardiology (DGPK) was held ..
  72. ncbi Diacylglycerol kinase zeta is associated with chromatin, but dissociates from condensed chromatin during mitotic phase in NIH3T3 cells
    Hiroshi Hasegawa
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990 9585, Japan
    J Cell Biochem 105:756-65. 2008
    Diacylglycerol kinase (DGK) converts diacylglycerol (DG) to phosphatidic acid, both of which act as second messengers to mediate a variety of cellular mechanisms...
  73. ncbi Endocannabinoids and diacylglycerol kinase activity
    Arpita Gantayet
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    Biochim Biophys Acta 1808:1050-3. 2011
    ....
  74. ncbi Overexpression of a rice diacylglycerol kinase gene OsBIDK1 enhances disease resistance in transgenic tobacco
    Weidong Zhang
    State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University at Huajiachi Campus, Hangzhou, Zhejiang 310029, People s Republic of China
    Mol Cells 26:258-64. 2008
    A rice diacylglycerol kinase (DGK) gene, OsBIDK1, which encodes a 499-amino acid protein, was cloned and characterized...
  75. ncbi Effect of phosphate on aluminium-inhibited growth and signal transduction pathways in Coffea arabica suspension cells
    Leticia Chee-González
    Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Merida, Yucatan, Mexico
    J Inorg Biochem 103:1497-503. 2009
    ..and phosphatidic acid (PA) by blocking PLC activity; however, phospholipase D (PLD) and diacylglycerol kinase (DGK) activities are stimulated by Al, a response probably aimed at counteracting Al effects on PA formation...
  76. ncbi Diacylglycerol kinase-alpha phosphorylation by Src on Y335 is required for activation, membrane recruitment and Hgf-induced cell motility
    G Baldanzi
    Department of Medical Sciences, University Amedeo Avogadro of Piemonte Oriental, Novara, Italy
    Oncogene 27:942-56. 2008
    Diacylglycerol (DAG) kinases (Dgk), which phosphorylate DAG to generate phosphatidic acid, act as either positive or negative key regulators of cell signaling...
  77. ncbi Cloning and expression of human mitochondrial deoxyguanosine kinase cDNA
    L Wang
    Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden
    FEBS Lett 390:39-43. 1996
    Mammalian mitochondrial deoxyguanosine kinase (dGK) is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix...
  78. ncbi Adenovirus-mediated overexpression of diacylglycerol kinase-zeta inhibits endothelin-1-induced cardiomyocyte hypertrophy
    Hiroki Takahashi
    First Department of Internal Medicine, Yamagata University School of Medicine, Yamagata, Japan
    Circulation 111:1510-6. 2005
    ..Diacylglycerol kinase (DGK) is thought to be an enzyme that controls the cellular levels of DAG by converting it to phosphatidic acid; however,..
  79. ncbi Translocation of diacylglycerol kinase theta from cytosol to plasma membrane in response to activation of G protein-coupled receptors and protein kinase C
    Jürgen van Baal
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands
    J Biol Chem 280:9870-8. 2005
    Diacylglycerol kinase (DGK) phosphorylates the second messenger diacylglycerol (DAG) to phosphatidic acid...
  80. ncbi Modulation of the mammalian target of rapamycin pathway by diacylglycerol kinase-produced phosphatidic acid
    Antonia Avila-Flores
    Department of Immunology and Oncology, National Centre for Biotechnology, Consejo Superior de Investigaciones Cientificas, Campus Cantoblanco, E 28049 Madrid, Spain
    J Biol Chem 280:10091-9. 2005
    ..Here we explored the contribution to this pathway of the enzyme diacylglycerol kinase (DGK), which produces PA through phosphorylation of diacylglycerol...
  81. ncbi Diacylglycerol kinase zeta regulates phosphatidylinositol 4-phosphate 5-kinase Ialpha by a novel mechanism
    Bai Luo
    Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    Cell Signal 16:891-7. 2004
    ..that expression of DGKzeta significantly enhances PIP5KIalpha activity in thrombin-stimulated HEK293 cells, and DGK activity is required for this stimulation...
  82. ncbi Secondary structure and backbone dynamics of Escherichia coli diacylglycerol kinase, as revealed by site-directed solid-state 13C NMR
    Satoru Yamaguchi
    Department of Life Science, Himeji Institute of Technology, Harima Science Garden City, Kamigori, Hyogo 678 1297, Japan
    Biochim Biophys Acta 1698:97-105. 2004
    ..we have recorded (13)C NMR spectra of [3-(13)C]Ala-, [1-(13)C]Val-labeled Escherichia coli diacylglycerol kinase (DGK), using cross-polarization-magic angle spinning (CP-MAS) and single-pulse excitation with dipolar decoupled-magic ..
  83. ncbi T cell activation in vivo targets diacylglycerol kinase alpha to the membrane: a novel mechanism for Ras attenuation
    Miguel A Sanjuan
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, Madrid, Spain
    J Immunol 170:2877-83. 2003
    Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid, leading to decreased and increased levels, respectively, of these two lipid messengers that play a central role in T cell activation...
  84. ncbi Phorbol ester-regulated oligomerization of diacylglycerol kinase delta linked to its phosphorylation and translocation
    Shin ichi Imai
    Department of Biochemistry, Sapporo Medical University School of Medicine, South 1, West 17, Chuo Ku, Sapporo 060 8556, Japan
    J Biol Chem 277:35323-32. 2002
    Diacylglycerol kinase (DGK) plays an important role in signal transduction through modulating the balance between two signaling lipids, diacylglycerol and phosphatidic acid...
  85. ncbi The deoxyguanosine kinase gene is mutated in individuals with depleted hepatocerebral mitochondrial DNA
    H Mandel
    Metabolic Disease Unit, Department of Pediatrics, Rambam Medical Center, Technion Israel Institute of Technology, Bruce Rappaport Faculty of Medicine, Haifa, Israel
    Nat Genet 29:337-41. 2001
    ..This interval encompasses the gene (DGUOK) encoding the mitochondrial deoxyguanosine kinase (dGK)...
  86. ncbi Nuclear diacylglycerol kinase-theta is activated in response to alpha-thrombin
    L Bregoli
    Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 276:23288-95. 2001
    ..Diacylglycerol kinase (DGK) catalyzes the conversion of diacylglycerol to phosphatidic acid, making it an attractive candidate for a signal ..
  87. ncbi Apoptosis induces efflux of the mitochondrial matrix enzyme deoxyguanosine kinase
    M Jullig
    Department of Veterinary Medical Chemistry, University of Agricultural Sciences, The Biomedical Centre, Box 575, S 751 23 Uppsala, Sweden
    J Biol Chem 276:24000-4. 2001
    Deoxyguanosine kinase (dGK) initiates the salvage of purine deoxynucleosides in mitochondria and is a key enzyme in mitochondrial DNA precursor synthesis...
  88. ncbi Serotonin inhibition of synaptic transmission: Galpha(0) decreases the abundance of UNC-13 at release sites
    S Nurrish
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 24:231-42. 1999
    ..The effects of serotonin on synaptic transmission were mediated by GOA-1 (a Galpha0 subunit) and DGK-1 (a diacylglycerol [DAG] kinase), both of which act in the ventral cord motor neurons...
  89. ncbi An N-terminal fragment of ProSAAS (a granin-like neuroendocrine peptide precursor) is associated with tau inclusions in Pick's disease
    Kenji Kikuchi
    Third Department of Internal Medicine, Yamagata University School of Medicine, Japan
    Biochem Biophys Res Commun 308:646-54. 2003
    ..analysis of the composition of tau inclusions, we detected the intense binding of anti-diacylglycerol kinase-zeta (DGK-zeta) antibodies to Pick bodies (PBs), which represent tau inclusions in Pick's disease...
  90. ncbi Diacylglycerol kinase theta binds to and is negatively regulated by active RhoA
    B Houssa
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 274:6820-2. 1999
    Diacylglycerol kinase (DGK) phosphorylates the second messenger diacylglycerol to yield phosphatidic acid. To date, very little is known about the regulation of DGK activity...
  91. ncbi Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases
    F Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, South 1, West 17, Sapporo 060, Japan
    J Biol Chem 271:8394-401. 1996
    A fourth member of the diacylglycerol kinase (DGK) gene family termed DGK delta was cloned from the human testis cDNA library...
  92. ncbi The cloning and characterization of a novel human diacylglycerol kinase, DGKiota
    L Ding
    Huntsman Cancer Institute, Eccles Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City, Utah, USA
    J Biol Chem 273:32746-52. 1998
    ..plays a central role in both the synthesis of complex lipids and in intracellular signaling; diacylglycerol kinase (DGK) catalyzes the phosphorylation of DAG, which yields phosphatidic acid...
  93. ncbi Immunolocalization of Drosophila eye-specific diacylgylcerol kinase, rdgA, which is essential for the maintenance of the photoreceptor
    I Masai
    Molecular Genetics Research Laboratory, University of Tokyo, Japan
    J Neurobiol 32:695-706. 1997
    ..biochemical and molecular studies suggested that the rdgA gene encodes an eye-specific diacylglycerol kinase (DGK)...
  94. ncbi Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alte
    Fernando C Gómez-Merino
    University of Potsdam, Institute of Biochemistry and Biology, Karl Liebknecht Strasse 24 25, Haus 20, Golm Potsdam D 14476, Germany
    J Biol Chem 280:34888-99. 2005
    Diacylglycerol kinase (DGK) regulates the level of the second messenger diacylglycerol and produces phosphatidic acid (PA), another signaling molecule...
  95. ncbi Cardiac-specific overexpression of diacylglycerol kinase zeta prevents Gq protein-coupled receptor agonist-induced cardiac hypertrophy in transgenic mice
    Takanori Arimoto
    First Department of Internal Medicine, Yamagata University School of Medicine, Yamagata, Japan
    Circulation 113:60-6. 2006
    ..Diacylglycerol functions as a potent activator of protein kinase C (PKC) and is catalyzed by diacylglycerol kinase (DGK) to form phosphatidic acid and inactivated...
  96. ncbi Targeting of diacylglycerol degradation to M1 muscarinic receptors by beta-arrestins
    Christopher D Nelson
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Science 315:663-6. 2007
    ..of DAG to phosphatidic acid after agonist stimulation, and this activity requires recruitment of the beta-arrestin-DGK complex to activated 7TMRs...
  97. ncbi Expression of deoxynucleoside kinases and 5'-nucleotidases in mouse tissues: implications for mitochondrial toxicity
    Svetlana N Rylova
    Department of Anatomy, Physiology and Biochemistry, Section of Veterinary Medical Biochemistry, SLU, The Biomedical Center, P O Box 575, SE 751 23 Uppsala, Sweden
    Biochem Pharmacol 74:169-75. 2007
    ..1.74) and thymidine kinase (TK1; EC 2.7.1.21), the mitochondrial thymidine kinase (TK2) and deoxyguanosine kinase (dGK; EC 2.7.1.113) as well as 5'-deoxynucleotidases (5'-dNT; EC 3.1.3...
  98. ncbi Diacylglycerol kinase zeta attenuates pressure overload-induced cardiac hypertrophy
    Mutsuo Harada
    Department of Cardiology, Pulmonology and Nephrology, Yamagata University School of Medicine, Japan
    Circ J 71:276-82. 2007
    ..DAG kinase (DGK) phosphorylates DAG and controls cellular DAG levels, thus acting as a regulator of GPCR signaling...
  99. ncbi Nuclear diacylglycerol kinase-zeta is a negative regulator of cell cycle progression in C2C12 mouse myoblasts
    Camilla Evangelisti
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Cell Signalling Laboratory, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    FASEB J 21:3297-307. 2007
    ..It has been demonstrated that nuclear DGK-zeta interferes with cell cycle progression...
  100. ncbi Differential subcellular targeting and activity-dependent subcellular localization of diacylglycerol kinase isozymes in transfected cells
    Naoki Kobayashi
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Eur J Cell Biol 86:433-44. 2007
    Diacylglycerol kinase (DGK) plays a pivotal role in cellular signal transduction through regulating levels of the second messenger diacylglycerol (DG)...
  101. ncbi Novel selective human mitochondrial kinase inhibitors: design, synthesis and enzymatic activity
    Nunzia Ciliberti
    Department of Pharmaceutical Sciences, University of Ferrara, Italy
    Bioorg Med Chem 15:3065-81. 2007
    ..the contrary, modifications at 2'-OH position of araC and araG, have provided inactive derivatives against TK2 and dGK, respectively...

Research Grants50

  1. ENZYMOLOGY OF GOLGI STACK FORMATION
    Vivek Malhotra; Fiscal Year: 2007
    ..DAG generated as a result is metabolized, post-fission, by PKD dependent activation of diacylglycerol kinase(DGK)0 (aim#2)...
  2. Molecular Dissection of T Cell Anergy
    Thomas Gajewski; Fiscal Year: 2005
    ..One of these is diacylglycerol kinase (DGK)...
  3. DGK in T Cell Regulation and Tolerance
    Thomas F Gajewski; Fiscal Year: 2010
    ..I cells have been characterized to have a defect in TCR/CD28- L]' (Ti 'D- of the diacylglycerol kinase (DGK) family, in particular DGK-u, participate in the suppression of targeted genes in the post-thymic I cell ..
  4. DISSECTING G PROTEIN PATHWAYS
    Joshua Kaplan; Fiscal Year: 2000
    ..We have cloned three of these downstream genes. dgk-1 and egl-36 are downstream of GOA-1 and encode a diacylglycerol kinase and a Shaw type potassium channel subunit, ..
  5. Regulatory Pathways in Osteoclasts and Immune Cells during Inflammatory Arthritis
    Roberta Faccio; Fiscal Year: 2010
    ..In order to study the role of DAG, we now turned our attention to DGK?, an enzyme that decreases cellular levels of DAG by converting it to phosphatidic acid (PA)...
  6. Mechanistic Analysis of T Cell Polarity by Photoactivation of Single Cells
    MORGAN A HUSE; Fiscal Year: 2010
    ..that are required for stable DAG accumulation and reorientation of the MTOC;2) Determine the roles played by DGK-1 and DGK-6 in shaping the polarizing DAG gradient;and 3) Determine the importance of Marcksl1 dynamics during MTOC ..
  7. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2005
    ..in the genes that we have found to be critical in seizures (arachidonoyl-diglyceride-selective diglyceride kinase (DGK) epsilon and PAF receptor) using several approaches including kindling epileptogenesis...
  8. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2007
    ..aims are to: 1) test the hypothesis that deficiency in PAF-receptor and/or in arachidonyl-diglyceride-selective DGK-e result in decreased susceptibility to epileptogenesis; 2) identify upstream signaling modified as a consequence ..
  9. Vitamin D Hormone: Function and Mechanism of Action
    Sylvia Christakos; Fiscal Year: 2007
    ..TRPV6, as well as the functional significance of intestinal calbindin, using recently generated TRPV6 and calbindin-Dgk null mice...
  10. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009
    ..We believe that an understanding of nuclear lipid signaling is key to our ability to intervene in events such as wound repair and tissue remodeling. ..
  11. SIGNALING CASCADE OF RHOA MEDIATED PLD ACTIVATION
    DANIEL RABEN; Fiscal Year: 2003
    ..This will be accomplished by examining the ability of chimeric RhoA constructs, in which the N-terminus or C- terminus is replaced by the corresponding regions in other small G proteins, on targeting and PLD activation. ..
  12. A Murine Model of Chronic Autoimmune Hepatitis and Liver Fibrosis
    XIAOPING ZHONG; Fiscal Year: 2010
    ..The proposed studies are expected to improve understanding of the pathogenesis of autoimmune hepatitis and provide therapeutic strategies for AIH and other autoimmune diseases. ..
  13. Diacylglycerol Kinase Delta in Growth and Development
    MATTHEW KENT TOPHAM; Fiscal Year: 2010
    ..We made diacylglycerol kinase (DGK) delta knockout mice and discovered that by regulating protein kinase C enzymes, DGKdelta modulates the expression ..
  14. Regulation of peripheral T cell tolerance
    XIAOPING ZHONG; Fiscal Year: 2010
    ..In aim 2, we will investigate mechanisms by which DGK activity contributes to T cell anergy...
  15. Diacylglycerol Kinase Delta in Growth and Development
    MATTHEW TOPHAM; Fiscal Year: 2006
    ..Because cells often express several DGK inhibitors- which can inhibit only three DGKs- it has been very difficult to determine the function of each DGK ..
  16. A Murine Model of Chronic Autoimmune Hepatitis and Liver Fibrosis
    XIAOPING ZHONG; Fiscal Year: 2009
    ..The proposed studies are expected to improve understanding of the pathogenesis of autoimmune hepatitis and provide therapeutic strategies for AIH and other autoimmune diseases. ..