DGCR8

Summary

Gene Symbol: DGCR8
Description: DGCR8, microprocessor complex subunit
Alias: C22orf12, DGCRK6, Gy1, pasha, microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region gene 8
Species: human
Products:     DGCR8

Top Publications

  1. Gregory R, Yan K, Amuthan G, Chendrimada T, Doratotaj B, Cooch N, et al. The Microprocessor complex mediates the genesis of microRNAs. Nature. 2004;432:235-40 pubmed
    ..The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8, the product of a gene deleted in DiGeorge syndrome...
  2. Landthaler M, Yalcin A, Tuschl T. The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Curr Biol. 2004;14:2162-7 pubmed
    ..Here, we characterize the human DGCR8, the DiGeorge syndrome critical region gene 8, and its Drosophila melanogaster homolog...
  3. Sohn S, Bae W, Kim J, Yeom K, Kim V, Cho Y. Crystal structure of human DGCR8 core. Nat Struct Mol Biol. 2007;14:847-53 pubmed
    A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process...
  4. Shiohama A, Sasaki T, Noda S, Minoshima S, Shimizu N. Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins. Exp Cell Res. 2007;313:4196-207 pubmed
    We identified 11 proteins that are associated with DGCR8 by immunoprecipitation assay and mass spectrometry...
  5. Macias S, Plass M, Stajuda A, Michlewski G, Eyras E, Cáceres J. DGCR8 HITS-CLIP reveals novel functions for the Microprocessor. Nat Struct Mol Biol. 2012;19:760-6 pubmed publisher
    The Drosha-DGCR8 complex (Microprocessor) is required for microRNA (miRNA) biogenesis. DGCR8 recognizes the RNA substrate, whereas Drosha functions as the endonuclease...
  6. Han J, Lee Y, Yeom K, Nam J, Heo I, Rhee J, et al. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell. 2006;125:887-901 pubmed
    The Drosha-DGCR8 complex initiates microRNA maturation by precise cleavage of the stem loops that are embedded in primary transcripts (pri-miRNAs)...
  7. Faller M, Matsunaga M, Yin S, Loo J, Guo F. Heme is involved in microRNA processing. Nat Struct Mol Biol. 2007;14:23-9 pubmed
    ..Here we show that the RNA-binding protein DiGeorge critical region-8 (DGCR8), which is essential for the first processing step, is a heme-binding protein...
  8. Han J, Lee Y, Yeom K, Kim Y, Jin H, Kim V. The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev. 2004;18:3016-27 pubmed
    ..the action mechanism of human Drosha by generating mutants and by characterizing its new interacting partner, DGCR8. The basic action mechanism of Drosha was found to be similar to that of human Dicer; the RNase III domains A and ..
  9. Shenoy A, Blelloch R. Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8. PLoS ONE. 2009;4:e6971 pubmed publisher
    The Microprocessor, containing the RNA binding protein Dgcr8 and RNase III enzyme Drosha, is responsible for processing primary microRNAs to precursor microRNAs...

More Information

Publications109 found, 100 shown here

  1. Barr I, Smith A, Senturia R, Chen Y, Scheidemantle B, Burstyn J, et al. DiGeorge critical region 8 (DGCR8) is a double-cysteine-ligated heme protein. J Biol Chem. 2011;286:16716-25 pubmed publisher
    ..We previously found that DiGeorge Critical Region 8 (DGCR8), an essential microRNA processing factor, associates with heme of unknown redox state when overexpressed in ..
  2. Sand M, Skrygan M, Georgas D, Arenz C, Gambichler T, Sand D, et al. Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer. Mol Carcinog. 2012;51:916-22 pubmed publisher
    ..In this study, we investigated the expression profiles of the microprocessor complex subunit DiGeorge syndrome critical region gene 8 (DGCR8) and the RISC components argonaute-1 (AGO1), argonaute-2 (AGO2), as well as double-..
  3. Wada T, Kikuchi J, Furukawa Y. Histone deacetylase 1 enhances microRNA processing via deacetylation of DGCR8. EMBO Rep. 2012;13:142-9 pubmed publisher
    Relatively little is known about the regulatory mechanisms of the Drosha/DGCR8 complex, which processes miRNAs at the initial step of biogenesis...
  4. Han J, Pedersen J, Kwon S, BELAIR C, Kim Y, Yeom K, et al. Posttranscriptional crossregulation between Drosha and DGCR8. Cell. 2009;136:75-84 pubmed publisher
    The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate...
  5. Shiohama A, Sasaki T, Noda S, Minoshima S, Shimizu N. Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region. Biochem Biophys Res Commun. 2003;304:184-90 pubmed
    We have identified and cloned a novel gene (DGCR8) from the human chromosome 22q11.2. This gene is located in the DiGeorge syndrome chromosomal region (DGCR)...
  6. Gong M, Chen Y, Senturia R, Ulgherait M, Faller M, Guo F. Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8. Protein Sci. 2012;21:797-808 pubmed publisher
    b>DGCR8 (DiGeorge Critical Region 8) is an essential microRNA (miRNA) processing protein that recognizes primary transcripts of miRNAs (pri-miRNAs) and triggers their cleavage by the Drosha nuclease...
  7. Sundermeier T, Sakami S, Sahu B, Howell S, Gao S, Dong Z, et al. MicroRNA-processing Enzymes Are Essential for Survival and Function of Mature Retinal Pigmented Epithelial Cells in Mice. J Biol Chem. 2017;292:3366-3378 pubmed publisher
    ..mature RPE cell-specific Cre recombinase drivers to inactivate either Dicer1 or DiGeorge syndrome critical region 8 (Dgcr8), thus removing RPE miRNA regulatory activity in mice by disrupting two independent ..
  8. Mohanty V, Shah A, Allender E, Siddiqui M, Monick S, Ichi S, et al. Folate Receptor Alpha Upregulates Oct4, Sox2 and Klf4 and Downregulates miR-138 and miR-let-7 in Cranial Neural Crest Cells. Stem Cells. 2016;34:2721-2732 pubmed publisher
    ..Co-immunoprecipitation data suggests that FRα interacts with the Drosha-DGCR8 complex to affect pre-miRNA processing...
  9. Ohana R, Weiman Kelman B, Raviv S, Tamm E, Pasmanik Chor M, Rinon A, et al. MicroRNAs are essential for differentiation of the retinal pigmented epithelium and maturation of adjacent photoreceptors. Development. 2015;142:2487-98 pubmed publisher
    ..Here, through RPE-specific conditional mutagenesis of Dicer1 or Dgcr8 in mice, the importance of miRNAs for RPE differentiation was uncovered...
  10. Keverne E. Genomic imprinting, action, and interaction of maternal and fetal genomes. Proc Natl Acad Sci U S A. 2015;112:6834-40 pubmed publisher
    ..SRY is a hybrid gene of Dgcr8 expressed in the developing placenta and Sox3 expressed in hypothalamic development...
  11. Wang J, Zhang Y, Guo Z, Li R, Xue X, Sun Z, et al. Effects of perinatal fluoride exposure on the expressions of miR-124 and miR-132 in hippocampus of mouse pups. Chemosphere. 2018;197:117-122 pubmed publisher
    ..NaF significantly elevated the expression levels of miR-124, miR-132, and DiGeorge syndrome chromosomal region 8 (DGCR8) in hippocampus of mouse pups at postnatal day (PND) 21...
  12. Zhu L, Gao J, Huang K, Luo Y, Zhang B, Xu W. miR-34a screened by miRNA profiling negatively regulates Wnt/β-catenin signaling pathway in Aflatoxin B1 induced hepatotoxicity. Sci Rep. 2015;5:16732 pubmed publisher
    ..Down-regulated of Drosha, DGCR8 and Dicer 1 indicated an impairment of miRNA biogenesis in response to AFB1...
  13. Nowotny M, Yang W. Structural and functional modules in RNA interference. Curr Opin Struct Biol. 2009;19:286-93 pubmed publisher
    ..nucleic acid complexes involved in RNA biogenesis, for example, Argonaute, PIWI, RNase III, Dicer, Drosha, and DGCR8. While quite a few questions remain, an excellent structural and mechanistic overview of RNAi processes has ..
  14. Son D, Jung Y, Seo Y, Park H, Lee D, Kim S, et al. Interleukin-32α Inhibits Endothelial Inflammation, Vascular Smooth Muscle Cell Activation, and Atherosclerosis by Upregulating Timp3 and Reck through suppressing microRNA-205 Biogenesis. Theranostics. 2017;7:2186-2203 pubmed publisher
    ..the atheroprotective genes Timp3 and Reck by downregulating microRNA-205 through regulation of the Rprd2-Dgcr8/Ddx5-Dicer1 biogenesis pathway...
  15. Cordiner R, Macias S. Purification of Microprocessor-Associated Factors. Methods Mol Biol. 2018;1823:51-62 pubmed publisher
    ..The minimal catalytically active complex is formed by two essential factors, the dsRNA binding protein DGCR8, and the RNase III endonuclease Drosha...
  16. Zhang X, Gee H, Rose B, Lee C, Clark J, Elliott M, et al. Regulation of the tumour suppressor PDCD4 by miR-499 and miR-21 in oropharyngeal cancers. BMC Cancer. 2016;16:86 pubmed publisher
    ..expression of the miRNA machinery, Dicer1, Drosha, DDX5 (Dead Box Helicase 5) and DGCR8 (DiGeorge Syndrome Critical Region Gene 8) were all elevated by greater than 2 fold in the tonsil SCC tissue...
  17. Earls L, Fricke R, Yu J, Berry R, Baldwin L, Zakharenko S. Age-dependent microRNA control of synaptic plasticity in 22q11 deletion syndrome and schizophrenia. J Neurosci. 2012;32:14132-44 pubmed publisher
    ..Screening of multiple mutant mouse lines revealed that haploinsufficiency of Dgcr8, a microRNA (miRNA) biogenesis gene in the 22q11DS disease-critical region, causes age-dependent, synaptic SERCA2 ..
  18. Lee H, Han S, Kwon C, Lee D. Biogenesis and regulation of the let-7 miRNAs and their functional implications. Protein Cell. 2016;7:100-13 pubmed publisher
    ..a let-7 miRNA, a primary transcript is produced by RNA polymerase II and then subsequently processed by Drosha/DGCR8, TUTase, and Dicer...
  19. Eom T, Bayazitov I, Anderson K, Yu J, Zakharenko S. Schizophrenia-Related Microdeletion Impairs Emotional Memory through MicroRNA-Dependent Disruption of Thalamic Inputs to the Amygdala. Cell Rep. 2017;19:1532-1544 pubmed publisher
    ..This synaptic deficit is caused by haploinsufficiency of the 22q11DS gene Dgcr8, which is involved in microRNA processing, and is mediated by the increased dopamine receptor Drd2 levels in the ..
  20. Pedrosa E, Sandler V, Shah A, CARROLL R, Chang C, Rockowitz S, et al. Development of patient-specific neurons in schizophrenia using induced pluripotent stem cells. J Neurogenet. 2011;25:88-103 pubmed publisher
    ..Constitutive expression of OCT4 has been observed in Dgcr8-deficient mouse embryonic stem cells (mESCs); DGCR8 maps to the 22q11.2-deleted region...
  21. Moura R, Vaz Cunha P, Silva Gonçalves C, Correia Pinto J. Characterization of miRNA processing machinery in the embryonic chick lung. Cell Tissue Res. 2015;362:569-75 pubmed publisher
    ..The present work characterizes the expression pattern of drosha, dgcr8, exportin-5 and dicer1 in early stages of the embryonic chick lung by whole mount in situ hybridization and cross-..
  22. Wang F, Song W, Zhao H, Ma Y, Li Y, Zhai D, et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res. 2017;27:416-439 pubmed publisher
    ..QKI5 recognizes a distal QKI response element and recruits Microprocessor through interaction with DGCR8. Furthermore, the recruited Microprocessor is brought to pri-124-1 stem loops by a spatial RNA-RNA interaction ..
  23. Sisti F, Wang S, Brandt S, Glosson Byers N, Mayo L, Son Y, et al. Nuclear PTEN enhances the maturation of a microRNA regulon to limit MyD88-dependent susceptibility to sepsis. Sci Signal. 2018;11: pubmed publisher
    ..that PTEN induced miRNA production by associating with and facilitating the nuclear localization of Drosha-Dgcr8, part of the miRNA-processing complex...
  24. Hube F, Ulveling D, Sureau A, Forveille S, Francastel C. Short intron-derived ncRNAs. Nucleic Acids Res. 2017;45:4768-4781 pubmed publisher
    ..by splicing, and tested their dependence on each key factor in canonical or alternative miRNAs biogenesis (Drosha, DGCR8, DBR1, snRNP70, U2AF65, PRP8, Dicer, Ago2)...
  25. Carraco G, Gonçalves A, Serra C, Andrade R. MicroRNA processing machinery in the developing chick embryo. Gene Expr Patterns. 2014;16:114-21 pubmed publisher
    ..Herein, we report a detailed characterization of the expression patterns of DROSHA, DGCR8, XPO5 and DICER1 in the developing chick embryo, from HH1 (when the egg is laid) to HH25 (5-days incubation), ..
  26. Fernandez Perez D, Brieño Enriquez M, Isoler Alcaraz J, Larriba E, del Mazo J. MicroRNA dynamics at the onset of primordial germ and somatic cells sex differentiation during mouse embryonic gonad development. RNA. 2017;: pubmed publisher
    ..addition uncovered sexually and developmentally specific expression, char-acterized by the decay of Drosha, Dgcr8 and Xpo5 expression along gonadal develop-ment...
  27. Meseure D, Vacher S, Lallemand F, Alsibai K, Hatem R, Chemlali W, et al. Prognostic value of a newly identified MALAT1 alternatively spliced transcript in breast cancer. Br J Cancer. 2016;114:1395-404 pubmed publisher
    ..expression was associated with alterations of the pre-mRNAs alternative splicing machinery, and of the Drosha-DGCR8 complex required for non-coding RNA biogenesis...
  28. Bråte J, Neumann R, Fromm B, Haraldsen A, Tarver J, Suga H, et al. Unicellular Origin of the Animal MicroRNA Machinery. Curr Biol. 2018;28:3288-3295.e5 pubmed publisher
    ..We identify in Ichthyosporea both Drosha and Pasha (DGCR8 in vertebrates), indicating that the Microprocessor complex evolved long before the last common ancestor of ..
  29. Candi E, Amelio I, Agostini M, Melino G. MicroRNAs and p63 in epithelial stemness. Cell Death Differ. 2015;22:12-21 pubmed publisher
    ..and indirectly regulates their processing by regulating the expression of the miR processing components Dicer and DGCR8. In this review, we will discuss the recent findings on the miR-p63 interaction in epidermal biology, particularly ..
  30. Ratnadiwakara M, Mohenska M, Ankö M. Splicing factors as regulators of miRNA biogenesis - links to human disease. Semin Cell Dev Biol. 2018;79:113-122 pubmed publisher
    ..In addition to Drosha/DGCR8 and Dicer that are the essential components of the miRNA processing pathway, a range of other RNA binding proteins ..
  31. Wei L, Wang Y, Chang Y, An N, Wang X, Zhou P, et al. Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing. Am J Cancer Res. 2018;8:226-244 pubmed
    ..Yes associated protein (YAP) nuclear translocation mediates sequestration of p72, a co-factor of the Drosha/DGCR8 complex for pri-miR-7s processing, leading to attenuation of microprocessors' efficiency...
  32. Zhao J, Qi X, Dai Q, He X, Dweep H, Guo M, et al. Toxicity study of ochratoxin A using HEK293 and HepG2 cell lines based on microRNA profiling. Hum Exp Toxicol. 2016;: pubmed
    ..b>DGCR8, Dicer1, and Drosha were significantly suppressed in HEK293 cells, indicating an impairment of miRNA biogenesis...
  33. Mori M, Triboulet R, Mohseni M, Schlegelmilch K, Shrestha K, Camargo F, et al. Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer. Cell. 2014;156:893-906 pubmed publisher
    ..Thus, the Hippo pathway links contact-inhibition regulation to miRNA biogenesis and may be responsible for the widespread miRNA repression observed in cancer. ..
  34. BELAIR C, Paikari A, Moltzahn F, Shenoy A, Yau C, Dall Era M, et al. DGCR8 is essential for tumor progression following PTEN loss in the prostate. EMBO Rep. 2015;16:1219-32 pubmed publisher
    ..Here, we show that deletion of the Dgcr8 gene, a critical component of this complex, inhibits tumor progression in a Pten-knockout mouse model of prostate ..
  35. Kourtidis A, Ngok S, Pulimeno P, Feathers R, Carpio L, Baker T, et al. Distinct E-cadherin-based complexes regulate cell behaviour through miRNA processing or Src and p120 catenin activity. Nat Cell Biol. 2015;17:1145-57 pubmed publisher
    ..defined by the p120 partner PLEKHA7 and a non-nuclear subset of the core microprocessor components DROSHA and DGCR8, and one growth promoting at basolateral areas of cell-cell contact containing tyrosine-phosphorylated p120 and ..
  36. Zhang Z, Guo X, Ge C, Ma Z, Jiang M, Li T, et al. KETCH1 imports HYL1 to nucleus for miRNA biogenesis in Arabidopsis. Proc Natl Acad Sci U S A. 2017;114:4011-4016 pubmed publisher
    ..leaves 1 (HYL1) protein, a core component of microprocessor in Arabidopsis and functional counterpart of DGCR8/Pasha in animals...
  37. Barr I, Weitz S, Atkin T, Hsu P, Karayiorgou M, Gogos J, et al. Cobalt(III) Protoporphyrin Activates the DGCR8 Protein and Can Compensate microRNA Processing Deficiency. Chem Biol. 2015;22:793-802 pubmed publisher
    ..In 22q11.2 deletion syndrome (22q11.2DS), heterozygous deletion of DiGeorge critical region gene 8 (DGCR8) causes a processing deficiency, which contributes to abnormal brain development...
  38. Link S, Grund S, Diederichs S. Alternative splicing affects the subcellular localization of Drosha. Nucleic Acids Res. 2016;44:5330-43 pubmed publisher
    ..Together with its co-factor DGCR8, it converts the primary transcript (pri-miRNA) into the precursor hairpin (pre-miRNA) in the nucleus...
  39. Castillo J, Urcuqui Inchima S. Letter to the Editor "Drosha, DGCR8, and Dicer mRNAs are downregulated in human cells infected with dengue virus 4" - Genet. Mol. Res. 15 (2): gmr.15027891 - Drosha, Dicer, and TRBP mRNA are downregulated in Vero cells with the 3'UTR of Dengue virus. Genet Mol Res. 2016;15: pubmed publisher
    ..to the downregulation of expression of key components of microRNA (miRNA) biogenesis, such as Drosha, Dicer, and DGCR8. For this, the authors performed a time course infection of A-549 cells for 5 days...
  40. Napoli E, Tassone F, Wong S, Angkustsiri K, Simon T, Song G, et al. Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome. J Biol Chem. 2015;290:23240-53 pubmed publisher
    ..Nine of ∼30 genes involved in 22qDS have the potential of disrupting mitochondrial metabolism (COMT, UFD1L, DGCR8, MRPL40, PRODH, SLC25A1, TXNRD2, T10, and ZDHHC8)...
  41. Liu Z, Skamagki M, Kim K, Zhao R. Canonical MicroRNA Activity Facilitates but May Be Dispensable for Transcription Factor-Mediated Reprogramming. Stem Cell Reports. 2015;5:1119-1127 pubmed publisher
    ..Here we report on successful reprogramming of mouse fibroblasts and neural stem cells (NSCs) lacking Dgcr8, a factor required for the biogenesis of canonical miRNAs, by Yamanaka factors, albeit at decreased efficiencies...
  42. Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
    ..They are generated following transcription and cleavage by the DROSHA/DGCR8 and DICER/TRBP/PACT complexes...
  43. Jiang Y, Chen J, Wu J, Hu Z, Qin Z, Liu X, et al. Evaluation of genetic variants in microRNA biosynthesis genes and risk of breast cancer in Chinese women. Int J Cancer. 2013;133:2216-24 pubmed publisher
    ..study, we systematically selected 24 functional SNPs located in eight key biosynthesis genes of miRNA (DROSHA, DGCR8, RAN, DICER, AGO2, GEMIN3, GEMIN4 and HIWI) and investigated the association between these SNPs and the risk of ..
  44. Bruzgielewicz A, Osuch Wójcikiewicz E, Walczak A, Nowak A, Uczkowski H, Majsterek I. Evaluation of polymorphisms in microRNA biosynthesis genes and risk of laryngeal cancer in the Polish population. Pol J Pathol. 2016;67:283-290 pubmed publisher
    ..Therefore, the aim of this study was to evaluate the relationship between DROSHA (rs6877842) and DGCR8 (rs417309, rs1640299) gene polymorphisms with risk of occurrence of laryngeal cancer...
  45. Chakraborty S, Krishnan Y. A structural map of oncomiR-1 at single-nucleotide resolution. Nucleic Acids Res. 2017;45:9694-9705 pubmed publisher
    ..regions of oncomiR-1 reveal that most of its primary microRNA domains are suboptimal substrates for Drosha-DGCR8, and therefore resistant to microprocessing...
  46. Krawczynski K, Bauersachs S, Reliszko Z, Graf A, Kaczmarek M. Expression of microRNAs and isomiRs in the porcine endometrium: implications for gene regulation at the maternal-conceptus interface. BMC Genomics. 2015;16:906 pubmed publisher
    ..Moreover, expression profiles of ten genes related to miRNA synthesis and transport such as DROSHA, DGCR8, XPO5, DICER, TARBP2, TNRC6A, and AGO1-4 were determined...
  47. Sellier C, Hwang V, Dandekar R, Durbin Johnson B, Charlet Berguerand N, Ander B, et al. Decreased DGCR8 expression and miRNA dysregulation in individuals with 22q11.2 deletion syndrome. PLoS ONE. 2014;9:e103884 pubmed publisher
    ..Here, we report on decreased expression levels of genes within the deletion region of chromosome 22, including DGCR8, in peripheral leukocytes derived from individuals with 22q11DS compared to healthy controls...
  48. Lin H, Oksuz I, Hurley E, Wrabetz L, Awatramani R. Microprocessor complex subunit DiGeorge syndrome critical region gene 8 (Dgcr8) is required for schwann cell myelination and myelin maintenance. J Biol Chem. 2015;290:24294-307 pubmed publisher
    We investigated the role of a key component of the Microprocessor complex, DGCR8, in the regulation of myelin formation and maintenance...
  49. Heras S, Macias S, Plass M, Fernandez N, Cano D, Eyras E, et al. The Microprocessor controls the activity of mammalian retrotransposons. Nat Struct Mol Biol. 2013;20:1173-81 pubmed publisher
    ..Here, we show that the Microprocessor (Drosha-DGCR8), which is required for microRNA biogenesis, also recognizes and binds RNAs derived from human long interspersed ..
  50. Liu Y, Zhu Z, Ho I, Shi Y, Xie Y, Li J, et al. Germline-specific dgcr8 knockout in zebrafish using a BACK approach. Cell Mol Life Sci. 2017;74:2503-2511 pubmed publisher
    ..We have successfully deleted the DiGeorge syndrome critical region gene 8 (dgcr8) in zebrafish germ line and demonstrated that the maternal-zygotic dgcr8 (MZdgcr8) embryos ..
  51. Yuan H, Deng R, Zhao X, Chen R, Hou G, Zhang H, et al. SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis. Mol Cancer. 2017;16:157 pubmed publisher
    ..We recently found that SUMOylation of TARBP2 and DGCR8 is involved in the regulation of the miRNA pathway...
  52. Lin H, Oksuz I, Svaren J, Awatramani R. Egr2-dependent microRNA-138 is dispensable for peripheral nerve myelination. Sci Rep. 2018;8:3817 pubmed publisher
    ..To fill this gap, we focused this study on miR-138, which was shown to be drastically reduced in Dicer1 and Dgcr8 knockout mice with hypomyelinating phenotypes and to potentially target the negative regulators of Schwann cell ..
  53. Ma J, Yang F, Zhou C, Liu F, Yuan J, Wang F, et al. METTL14 suppresses the metastatic potential of hepatocellular carcinoma by modulating N6 -methyladenosine-dependent primary MicroRNA processing. Hepatology. 2017;65:529-543 pubmed publisher
    ..We confirm that METTL14 interacts with the microprocessor protein DGCR8 and positively modulates the primary microRNA 126 process in an m6 A-dependent manner...
  54. Marinaro F, Marzi M, Hoffmann N, Amin H, Pelizzoli R, Niola F, et al. MicroRNA-independent functions of DGCR8 are essential for neocortical development and TBR1 expression. EMBO Rep. 2017;18:603-618 pubmed publisher
    Recent evidence indicates that the miRNA biogenesis factors DROSHA, DGCR8, and DICER exert non-overlapping functions, and have also roles in miRNA-independent regulatory mechanisms...
  55. Bartram M, Amendola E, Benzing T, Schermer B, De Vita G, Muller R. Mice lacking microRNAs in Pax8-expressing cells develop hypothyroidism and end-stage renal failure. BMC Mol Biol. 2016;17:11 pubmed publisher
    ..The miRNA machinery was disrupted by crossing conditional DiGeorge syndrome critical region 8 (Dgcr8) fl/fl mice to Pax8Cre mice...
  56. Brandl A, Daum P, Brenner S, Schulz S, Yap D, Bösl M, et al. The microprocessor component, DGCR8, is essential for early B-cell development in mice. Eur J Immunol. 2016;46:2710-2718 pubmed publisher
    ..in two steps by the microprocessor complex, consisting of Drosha and its partner DiGeorge Critical Region 8 (DGCR8), and the RNAse III enzyme, Dicer...
  57. Patne K, Rakesh R, Arya V, Chanana U, Sethy R, Swer P, et al. BRG1 and SMARCAL1 transcriptionally co-regulate DROSHA, DGCR8 and DICER in response to doxorubicin-induced DNA damage. Biochim Biophys Acta Gene Regul Mech. 2017;1860:936-951 pubmed publisher
    ..members of the ATP-dependent chromatin remodelling family, are shown to co-regulate the transcription of DROSHA, DGCR8, and DICER in response to double-strand DNA breaks induced by doxorubicin...
  58. Zheng Q, Yang H, Yuan Y. Autoantigen La Regulates MicroRNA Processing from Stem-Loop Precursors by Association with DGCR8. Biochemistry. 2017;56:6098-6110 pubmed publisher
    ..microRNA (pri-miRNA) processing starts from precise cleavage of the stem loop, which is catalyzed by the Drosha-DGCR8 complex...
  59. Partin A, Ngo T, Herrell E, Jeong B, Hon G, Nam Y. Heme enables proper positioning of Drosha and DGCR8 on primary microRNAs. Nat Commun. 2017;8:1737 pubmed publisher
    ..pri-miRs) are cleaved by Microprocessor, a complex containing the RNase Drosha and its partner protein, DGCR8. Although DGCR8 is known to bind heme, the molecular role of heme in pri-miR processing is unknown...
  60. Xiong Q, Ma B, Lu X, Huang Y, He S, Yang C, et al. Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings. Plant Cell. 2017;29:1053-1072 pubmed publisher
    ..Here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a gaoyao1 (gy1) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice...
  61. Senturia R, Faller M, Yin S, Loo J, Cascio D, Sawaya M, et al. Structure of the dimerization domain of DiGeorge critical region 8. Protein Sci. 2010;19:1354-65 pubmed publisher
    ..the Microprocessor complex containing the Drosha nuclease and the RNA-binding protein DiGeorge critical region 8 (DGCR8)...
  62. Bellemer C, Bortolin Cavaillé M, Schmidt U, Jensen S, Kjems J, Bertrand E, et al. Microprocessor dynamics and interactions at endogenous imprinted C19MC microRNA genes. J Cell Sci. 2012;125:2709-20 pubmed publisher
    Nuclear primary microRNA (pri-miRNA) processing catalyzed by the DGCR8-Drosha (Microprocessor) complex is highly regulated. Little is known, however, about how microRNA biogenesis is spatially organized within the mammalian nucleus...
  63. Casseb S, Simith D, Melo K, Mendonca M, Santos A, Carvalho V, et al. Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis. Genet Mol Res. 2016;15: pubmed publisher
    ..This study aims to investigate Drosha, DGCR8, and Dicer expression levels in human A-549 cells following DENV4 infection...
  64. Daugaard I, Hansen T. Biogenesis and Function of Ago-Associated RNAs. Trends Genet. 2017;33:208-219 pubmed publisher
    ..class of post-transcriptional regulators that follow a specific biogenesis pathway characterized by Drosha/DGCR8 and Dicer processing...
  65. Beveridge N, Gardiner E, Carroll A, Tooney P, Cairns M. Schizophrenia is associated with an increase in cortical microRNA biogenesis. Mol Psychiatry. 2010;15:1176-89 pubmed publisher
    ..with an elevation of primary microRNA processing and corresponded with an increase in the microprocessor component DGCR8. The biological implications for this extensive increase in gene silencing are profound, and were exemplified by ..
  66. Sato Y, Shinka T, Sakamoto K, Ewis A, Nakahori Y. The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2. Mol Cell Biochem. 2010;337:267-75 pubmed publisher
    ..amino acid homology, we realized that SRY is a hybrid gene between a portion of the first exon of DiGeorge syndrome critical region gene 8 (DGCR8) and the high-mobility group (HMG) box of SRY box-3 (SOX3) gene...
  67. Roth B, Ishimaru D, Hennig M. The core microprocessor component DiGeorge syndrome critical region 8 (DGCR8) is a nonspecific RNA-binding protein. J Biol Chem. 2013;288:26785-99 pubmed publisher
    ..Microprocessor, which consists of the nuclear RNase III Drosha and the double-stranded RNA-binding domain protein DGCR8 (DiGeorge syndrome critical region protein 8)...
  68. Herbert K, Sarkar S, Mills M, Delgado De la Herran H, Neuman K, Steitz J. A heterotrimer model of the complete Microprocessor complex revealed by single-molecule subunit counting. RNA. 2016;22:175-83 pubmed publisher
    ..miRNA) biogenesis, the Microprocessor complex (MC), composed minimally of Drosha, an RNaseIII enzyme, and DGCR8, a double-stranded RNA-binding protein, cleaves the primary-miRNA (pri-miRNA) to release the pre-miRNA stem-loop ..
  69. Görücü Yilmaz Ş, Erdal M, Avci Özge A, Sungur M. SNP Variation in MicroRNA Biogenesis Pathway Genes as a New Innovation Strategy for Alzheimer Disease Diagnostics: A Study of 10 Candidate Genes in an Understudied Population From the Eastern Mediterranean. Alzheimer Dis Assoc Disord. 2016;30:203-9 pubmed publisher
    ..528, P=0.104) and allele (P=0.035). Functional SNP variations in the other 8 candidate genes (DGCR8, XPO5, RAN, DICER1, AGO1, AGO2, GEMIN3, and GEMIN4) did not associate with AD in our sample...
  70. Jung E, Seong Y, Seo J, Kwon Y, Song H. Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex. Biochem Biophys Res Commun. 2014;446:241-7 pubmed publisher
    ..Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression. ..
  71. Hessam S, Sand M, Skrygan M, Gambichler T, Bechara F. Inflammation induced changes in the expression levels of components of the microRNA maturation machinery Drosha, Dicer, Drosha co-factor DGRC8 and Exportin-5 in inflammatory lesions of hidradenitis suppurativa patients. J Dermatol Sci. 2016;82:166-74 pubmed publisher
    ..Dicer and Exportin-5 may contribute to the later inflammatory process with visible HS lesions. ..
  72. Girvan H, Bradley J, Cheesman M, Kincaid J, Liu Y, Czarnecki K, et al. Analysis of Heme Iron Coordination in DGCR8: The Heme-Binding Component of the Microprocessor Complex. Biochemistry. 2016;55:5073-83 pubmed publisher
    b>DGCR8 is the RNA-binding partner of the nuclease Drosha. Their complex (the "Microprocessor") is essential for processing of long, primary microRNAs (pri-miRNAs) in the nucleus...
  73. Toyoshima M, Akamatsu W, Okada Y, Ohnishi T, Balan S, Hisano Y, et al. Analysis of induced pluripotent stem cells carrying 22q11.2 deletion. Transl Psychiatry. 2016;6:e934 pubmed publisher
    ..As an underlying mechanism, we focused on the role of DGCR8, a key gene for microRNA (miRNA) processing and mapped in the deleted region...
  74. Clague J, Lippman S, Yang H, Hildebrandt M, Ye Y, Lee J, et al. Genetic variation in MicroRNA genes and risk of oral premalignant lesions. Mol Carcinog. 2010;49:183-9 pubmed publisher
  75. Rostami Mogaddam M, Safavi Ardabili N, Shafaeei Y, Maleki N, Jafari N, Jafari A. Overexpression of Drosha, DiGeorge syndrome critical region gene 8 (DGCR8), and Dicer mRNAs in the pathogenesis of psoriasis. J Cosmet Dermatol. 2017;16:e48-e53 pubmed publisher
    ..Recent studies have indicated that microRNAs (miRNAs) play important roles in psoriasis...
  76. Choi Y, Jeong S, Yoon K, Sung H, Cho H, Kim D, et al. Deficiency of DGCR8 increases bone formation through downregulation of miR-22 expression. Bone. 2017;103:287-294 pubmed publisher
    ..Mature miRNAs are generated following sequential cleavage by DROSHA/DGCR8 and DICER. However, recent studies have identified that some miRNAs require only one of these enzymes...
  77. Gomez Cabello D, Adrados I, Gamarra D, Kobayashi H, Takatsu Y, Takatsu K, et al. DGCR8-mediated disruption of miRNA biogenesis induces cellular senescence in primary fibroblasts. Aging Cell. 2013;12:923-31 pubmed publisher
    ..global disruption of miRNA biogenesis in primary fibroblasts of human or murine origin, through the knockdown of DGCR8, an essential mediator of the synthesis of canonical miRNAs...
  78. Rios C, Warren D, Olson B, Abbott A. Functional analysis of microRNA pathway genes in the somatic gonad and germ cells during ovulation in C. elegans. Dev Biol. 2017;426:115-125 pubmed publisher
    ..Worms with reduced miRNA biogenesis due to loss of Drosha or Pasha/DGCR8 activity are sterile and fail to ovulate, indicating that miRNAs are required for the process of oocyte maturation ..
  79. Quick Cleveland J, Jacob J, Weitz S, Shoffner G, Senturia R, Guo F. The DGCR8 RNA-binding heme domain recognizes primary microRNAs by clamping the hairpin. Cell Rep. 2014;7:1994-2005 pubmed publisher
    ..and cleaved by the Microprocessor complex consisting of the Drosha nuclease and its obligate RNA-binding partner DGCR8. It is not well understood how the Microprocessor specifically recognizes pri-miRNA substrates...
  80. Jiang L, Shao C, Wu Q, Chen G, Zhou J, Yang B, et al. NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing. Nat Struct Mol Biol. 2017;24:816-824 pubmed publisher
    ..binds a large number of expressed pri-miRNAs in HeLa cells to globally enhance pri-miRNA processing by the Drosha-DGCR8 Microprocessor. NONO and PSF are key components of paraspeckles organized by the long noncoding RNA (lncRNA) NEAT1...
  81. Kim J, Choi Y, Jin G, Kang H, Choi J, Jeon H, et al. Association of a common AGO1 variant with lung cancer risk: a two-stage case-control study. Mol Carcinog. 2010;49:913-21 pubmed publisher
    ..In stage 1 of the study, 24 SNPs in the 11 miRNA biosynthesis genes (DROSHA, DGCR8, RAN, XPO5, DICER, AGO1, AGO2, HIWI, GEMIN3, GEMIN4, and TRBP) were genotyped in 100 lung cancer patients and 100 ..
  82. Liu Z, Zhang C, Skamagki M, Khodadadi Jamayran A, Zhang W, Kong D, et al. Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem Cells. Stem Cell Reports. 2017;9:1604-1617 pubmed publisher
    Pluripotent stem cells (PSCs) deficient for microRNAs (miRNAs), such as Dgcr8-/- or Dicer-/- embryonic stem cells (ESCs), contain no mature miRNA and cannot differentiate into somatic cells...
  83. Nguyen T, Jo M, Choi Y, Park J, Kwon S, Hohng S, et al. Functional Anatomy of the Human Microprocessor. Cell. 2015;161:1374-87 pubmed publisher
    MicroRNA (miRNA) maturation is initiated by Microprocessor composed of RNase III DROSHA and its cofactor DGCR8, whose fidelity is critical for generation of functional miRNAs...
  84. Hajarnis S, Yheskel M, Williams D, Brefort T, Glaudemans B, Debaix H, et al. Suppression of microRNA Activity in Kidney Collecting Ducts Induces Partial Loss of Epithelial Phenotype and Renal Fibrosis. J Am Soc Nephrol. 2018;29:518-531 pubmed publisher
    ..miRNAs, we generated three mouse models with CD-specific inactivation of key miRNA pathway genes Dicer, Dgcr8, and the entire Argonaute gene family (Ago1, 2, 3, and 4)...
  85. Sharma H, Estep M, Birerdinc A, Afendy A, Moazzez A, Elariny H, et al. Expression of genes for microRNA-processing enzymes is altered in advanced non-alcoholic fatty liver disease. J Gastroenterol Hepatol. 2013;28:1410-5 pubmed publisher
    ..For each patient, we profiled mRNA levels for three miRNA processing elements (Drosha, DGCR8, and Dicer1) and seven pri-miRNAs (pri-miR-125b-2, pri-miR-16-2, pri-miR-26a-1, pri-miR-26a-2, pri-miR-7-1, pri-..
  86. Orlowski S, Flees J, Anthony N, Dridi S. Differential expression of water channel- and noncoding RNA biogenesis-related genes in three lines of chickens under a short-term water restriction. Poult Sci. 2017;: pubmed publisher
    ..The expression of DGCR8 and TRBP1 was not affected by WR in any population; however, DGCR8 mRNA levels were significantly lower in RB1995 ..
  87. Weitz S, Gong M, Barr I, Weiss S, Guo F. Processing of microRNA primary transcripts requires heme in mammalian cells. Proc Natl Acad Sci U S A. 2014;111:1861-6 pubmed publisher
    b>DiGeorge syndrome critical region gene 8 (DGCR8) is the RNA-binding partner protein of the nuclease Drosha...
  88. Walz A, Ooms A, Gadd S, Gerhard D, Smith M, Guidry Auvil J, et al. Recurrent DGCR8, DROSHA, and SIX homeodomain mutations in favorable histology Wilms tumors. Cancer Cell. 2015;27:286-97 pubmed publisher
    ..histology Wilms tumors (FHWTs) to occur within SIX1/2 (7% of 534 tumors) and microRNA processing genes (miRNAPGs) DGCR8 and DROSHA (15% of 534 tumors)...
  89. Calses P, Dhillon K, Tucker N, Chi Y, Huang J, Kawasumi M, et al. DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing. Cell Rep. 2017;19:162-174 pubmed publisher
    Ultraviolet (UV) radiation is a carcinogen that generates DNA lesions. Here, we demonstrate an unexpected role for DGCR8, an RNA binding protein that canonically functions with Drosha to mediate microRNA processing, in the repair of UV-..
  90. Hines J, Smith A, Jacob J, Lukat Rodgers G, Barr I, Rodgers K, et al. CO and NO bind to Fe(II) DiGeorge critical region 8 heme but do not restore primary microRNA processing activity. J Biol Inorg Chem. 2016;21:1021-1035 pubmed
    The RNA-binding heme protein DiGeorge critical region 8 (DGCR8) and its ribonuclease partner Drosha cleave primary transcripts of microRNA (pri-miRNA) as part of the canonical microRNA (miRNA) processing pathway...
  91. Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng Q, Minami Y, et al. Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development. Proc Natl Acad Sci U S A. 2017;114:E7479-E7488 pubmed publisher
    ..embryos, CLOCK protein was absent despite the expression of Clock mRNA, which we showed was due to Dicer/Dgcr8-dependent translational suppression of CLOCK...