Gene Symbol: DFF45
Description: DNA fragmentation factor subunit alpha
Alias: DFF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, inhibitor of CAD
Species: human
Products:     DFF45

Top Publications

  1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell. 1997;89:175-84 pubmed
    ..In cells undergoing apoptosis, the 45 kDa subunit is cleaved in the same pattern as it is cleaved by caspase-3 in vitro. These data delineate a direct signal transduction pathway during apoptosis: caspase-3 to DFF to DNA fragmentation. ..
  2. Sharif Askari E, Alam A, Rheaume E, Beresford P, Scotto C, Sharma K, et al. Direct cleavage of the human DNA fragmentation factor-45 by granzyme B induces caspase-activated DNase release and DNA fragmentation. EMBO J. 2001;20:3101-13 pubmed
    ..this study, we demonstrate caspase-independent and direct cleavage of the 45 kDa unit of DNA fragmentation factor (DFF45) by GrB both in vitro and in vivo...
  3. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature. 1998;391:43-50 pubmed
    ..A caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD) have now been identified in the cytoplasmic fraction of mouse lymphoma cells...
  4. Leek J, Carr I, Bell S, Markham A, Lench N. Assignment of the DNA fragmentation factor gene (DFFA) to human chromosome bands 1p36.3-->p36.2 by in situ hybridization. Cytogenet Cell Genet. 1997;79:212-3 pubmed
  5. Liu X, Zou H, Widlak P, Garrard W, Wang X. Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1. J Biol Chem. 1999;274:13836-40 pubmed
    DNA fragmentation factor (DFF) is a heterodimeric protein composed of 45-kDa (DFF45) and 40-kDa (DFF40) subunits, a protein that mediates regulated DNA fragmentation and chromatin condensation in response to apoptotic signals...
  6. Gu J, Dong R, Zhang C, McLaughlin D, Wu M, Schlossman S. Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. J Biol Chem. 1999;274:20759-62 pubmed
    ..Here we described the identification and characterization of DFF35, an isoform of DFF45 comprised of 268 amino acids...
  7. Lugovskoy A, Zhou P, Chou J, McCarty J, Li P, Wagner G. Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis. Cell. 1999;99:747-55 pubmed
    ..condensation are mediated by the caspase-activated DFF40/ CAD nuclease, which is chaperoned and inhibited by DFF45/ICAD. CIDE proteins share a homologous regulatory CIDE-N domain with DFF40/CAD and DFF45/ ICAD...
  8. Otomo T, Sakahira H, Uegaki K, Nagata S, Yamazaki T. Structure of the heterodimeric complex between CAD domains of CAD and ICAD. Nat Struct Biol. 2000;7:658-62 pubmed
    ..complex between the CAD domain of caspase activated deoxyribonuclease (CAD) and the CAD domain of its inhibitor (ICAD), determined by nuclear magnetic resonance spectroscopy...
  9. Abel F, Sjöberg R, Ejeskär K, Krona C, Martinsson T. Analyses of apoptotic regulators CASP9 and DFFA at 1P36.2, reveal rare allele variants in human neuroblastoma tumours. Br J Cancer. 2002;86:596-604 pubmed
    The genes encoding Caspase-9 and DFF45 have both recently been mapped to chromosome region 1p36.2, that is a region alleged to involve one or several tumour suppressor genes in neuroblastoma tumours...

More Information

Publications106 found, 100 shown here

  1. Charrier L, Jarry A, Toquet C, Bou Hanna C, Chedorge M, Denis M, et al. Growth phase-dependent expression of ICAD-L/DFF45 modulates the pattern of apoptosis in human colonic cancer cells. Cancer Res. 2002;62:2169-74 pubmed
    ..ICAD expression was restricted to the functional ICAD-L/DFF45 isoform in colonic cancer cells as well as in human normal colonocytes...
  2. Konishi S, Ishiguro H, Shibata Y, Kudo J, Terashita Y, Sugiura H, et al. Decreased expression of DFF45/ICAD is correlated with a poor prognosis in patients with esophageal carcinoma. Cancer. 2002;95:2473-8 pubmed
    DNA fragmentation factor 45 (DFF45)/inhibotor of caspase activated DNAse (ICAD) forms a complex with DFF40/CAD and inhibits its DNA cleaving function during apoptosis...
  3. Sacco R, Tsutsumi T, Suzuki R, Otsuka M, Aizaki H, Sakamoto S, et al. Antiapoptotic regulation by hepatitis C virus core protein through up-regulation of inhibitor of caspase-activated DNase. Virology. 2003;317:24-35 pubmed
    ..genes, some of which are involved in cell growth or apoptosis such as inhibitor of caspase-activated DNase (ICAD), defender against cell death 1, tumor necrosis factor (TNF) receptor 1, and cytochrome c oxidase subunit VIII...
  4. Yan B, Wang H, Peng Y, Hu Y, Wang H, Zhang X, et al. A unique role of the DNA fragmentation factor in maintaining genomic stability. Proc Natl Acad Sci U S A. 2006;103:1504-9 pubmed
  5. Yan B, Wang H, Zhuo D, Li F, Kon T, Dewhirst M, et al. Apoptotic DNA fragmentation factor maintains chromosome stability in a P53-independent manner. Oncogene. 2006;25:5370-6 pubmed
    ..In this study, we constructed cell lines expressing a mutant ICAD (inhibitor of CAD) protein that is resistant to caspase cleavage and therefore constantly binds to DFF/CAD and inhibits DNA ..
  6. Fernández Galilea M, Pérez Matute P, Prieto Hontoria P, Houssier M, Burrell M, Langin D, et al. α-Lipoic acid treatment increases mitochondrial biogenesis and promotes beige adipose features in subcutaneous adipocytes from overweight/obese subjects. Biochim Biophys Acta. 2015;1851:273-81 pubmed publisher
    ..Therefore, our study suggests the ability of α-Lip to promote mitochondrial biogenesis and brown-like remodeling in cultured white subcutaneous adipocytes from overweight/obese donors. ..
  7. Yang X, Qian S, Yao K, Wang L, Liu Y, Dong F, et al. Multiresistant ST59-SCCmec IV-t437 clone with strong biofilm-forming capacity was identified predominantly in MRSA isolated from Chinese children. BMC Infect Dis. 2017;17:733 pubmed publisher
    ..0001). The prevalence of the biofilm associated genes among ST59-SCCmec IV-t437 clone was: icaA (100.0%), icaD (97.3%), fnbpA (100.0%), fnbpB (0), clfA (100%), clfB (100%), cna (2.7%), bbp (0), ebpS (88.5%), sdrC (78...
  8. Aslantas O, Demir C. Investigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis cases. J Dairy Sci. 2016;99:8607-8613 pubmed publisher
    ..5, 67, and 62.5% of the isolates were found to be biofilm producers, respectively. The percentage rate of icaA, icaD, and bap genes in Staph. aureus isolates were 86.6, 86.6, and 13.4%, respectively...
  9. Chen L, Chen Y, Cheng K, Chien T, Chan C, Tsao S, et al. Antiobesity effect of Lactobacillus reuteri 263 associated with energy metabolism remodeling of white adipose tissue in high-energy-diet-fed rats. J Nutr Biochem. 2018;54:87-94 pubmed publisher
    ..The results suggest that L. reuteri 263 may treat obesity through energy metabolism remodeling of WAT in the high-energy-diet-induced obese rats. ..
  10. Valero Muñoz M, Li S, Wilson R, Hulsmans M, Aprahamian T, Fuster J, et al. Heart Failure With Preserved Ejection Fraction Induces Beiging in Adipose Tissue. Circ Heart Fail. 2016;9:e002724 pubmed publisher
    ..These findings show that HFpEF is associated with beiging in white AT and with dysfunctional brown AT. ..
  11. Costa F, Belo N, Costa E, Andrade G, Pereira L, Carvalho I, et al. Frequency of enterotoxins, toxic shock syndrome toxin-1, and biofilm formation genes in Staphylococcus aureus isolates from cows with mastitis in the Northeast of Brazil. Trop Anim Health Prod. 2018;50:1089-1097 pubmed publisher
    ..aureus isolates, from which 25 (65.78%) carried toxin genes, including seb, sec, sed, tst, and icaD adhesion gene. These S. aureus isolates belong to 21 ribotypes and three S...
  12. Mottola C, Semedo Lemsaddek T, Mendes J, Melo Cristino J, Tavares L, Cavaco Silva P, et al. Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci. J Biomed Sci. 2016;23:33 pubmed publisher
    ..Staphylococcal isolates were positive for intercellular adhesins icaA and icaD, negative for biofilm associated protein bap and pantone-valentine leucocidin pvl. S...
  13. Wang S, Liang X, Yang Q, Fu X, Rogers C, Zhu M, et al. Resveratrol induces brown-like adipocyte formation in white fat through activation of AMP-activated protein kinase (AMPK) α1. Int J Obes (Lond). 2015;39:967-76 pubmed publisher
    ..Resveratrol induces brown-like adipocyte formation in iWAT via AMPKα1 activation and suggest that its beneficial antiobesity effects may be partly due to the browning of WAT and, as a consequence, increased oxygen consumption. ..
  14. Chajęcka Wierzchowska W, Zadernowska A, Gajewska J. S. epidermidis strains from artisanal cheese made from unpasteurized milk in Poland - Genetic characterization of antimicrobial resistance and virulence determinants. Int J Food Microbiol. 2019;294:55-59 pubmed publisher
    ..epidermidis isolates were found to be unable to form a biofilm. All strains producing strong biofilm harbored the icaD gene which occurred independently or in combination with the icaA...
  15. Emaneini M, Khoramrooz S, Shahsavan S, Dabiri H, Jabalameli F. Prevalence of Panton-Valentine leucocidin and phenotypic and genotypic characterization of biofilm formation among Staphylococcus aureus strains isolated from children with adenoid hypertrophy. Microb Pathog. 2015;89:150-3 pubmed publisher
    ..1%) isolates were positive for the gene eno, 13(76.4%) for icaA, 13 (76.4%) for icaD, 10 (58.8%) for fib, 10 (58.8%) for fnbB, 4(23.5%) for can, and 1(5.8%) for fnbA...
  16. Kędzierska H, Piekiełko Witkowska A. Splicing factors of SR and hnRNP families as regulators of apoptosis in cancer. Cancer Lett. 2017;396:53-65 pubmed publisher
    ..regulators of the extrinsic (Fas, caspase-8, caspase-2, c-FLIP) and the intrinsic pathway (Apaf-1, caspase-9, ICAD), genes encoding Bcl-2 proteins, IAPs, and p53 tumor suppressor...
  17. Wang J, Xu M, Wang X, Yang J, Gao L, Zhang Y, et al. Comparative Proteome Analysis Reveals Lipid Metabolism-Related Protein Networks in Response to Rump Fat Mobilization. Int J Mol Sci. 2018;19: pubmed publisher
    ..Our findings provide a reference for elucidating the complex molecular mechanisms underlying rump fat mobilization. ..
  18. Goswami A, Shah B, Kumar A, Rizvi M, Kumar S, Bhushan S, et al. Antiproliferative potential of a novel parthenin analog P16 as evident by apoptosis accompanied by down-regulation of PI3K/AKT and ERK pathways in human acute lymphoblastic leukemia MOLT-4 cells. Chem Biol Interact. 2014;222:60-7 pubmed publisher
    ..Further, activation of caspase-3 led to cleavage of its target proteins PARP-1 and ICAD, which resulted in apoptotic DNA damage...
  19. Wu C, Zhang H, Fan C, Wu P, Wei Q, Cai Y, et al. [Expressions of inflammatory and fibrogenic factors in perinephric and subcutaneous adipose tissues of patients with adrenocorticotropic hormone-independent Cushing's syndrome]. Nan Fang Yi Ke Da Xue Xue Bao. 2016;37:563-566 pubmed
  20. Ni C, Li C, Dong Y, Guo X, Zhang Y, Xie Z. Anesthetic Isoflurane Induces DNA Damage Through Oxidative Stress and p53 Pathway. Mol Neurobiol. 2017;54:3591-3605 pubmed publisher
    ..X, cleaved caspase-3, and nucleus translocation of CAD and decreased levels of inhibitor of CAD (ICAD) and p53. Isoflurane enhanced the nucleus level of ?H2A.X...
  21. Laiglesia L, Lorente Cebrián S, Prieto Hontoria P, Fernández Galilea M, Ribeiro S, Sainz N, et al. Eicosapentaenoic acid promotes mitochondrial biogenesis and beige-like features in subcutaneous adipocytes from overweight subjects. J Nutr Biochem. 2016;37:76-82 pubmed publisher
  22. Langhi C, Arias N, Rajamoorthi A, Basta J, Lee R, Baldán A. Therapeutic silencing of fat-specific protein 27 improves glycemic control in mouse models of obesity and insulin resistance. J Lipid Res. 2017;58:81-91 pubmed publisher
    ..These data suggest that partial reduction of FSP27 activity (e.g., using ASOs) might be exploited therapeutically in insulin-resistant obese or overweight patients. ..
  23. Qu Y, Zhao H, Nobrega D, Cobo E, Han B, Zhao Z, et al. Molecular epidemiology and distribution of antimicrobial resistance genes of Staphylococcus species isolated from Chinese dairy cows with clinical mastitis. J Dairy Sci. 2019;102:1571-1583 pubmed publisher
    ..aureus. Biofilm formation genes (icaA and icaD) were frequently detected in staphylococci isolated from bovine clinical mastitis...
  24. Qian H, Chen Y, Nian Z, Su L, Yu H, Chen F, et al. HDAC6-mediated acetylation of lipid droplet-binding protein CIDEC regulates fat-induced lipid storage. J Clin Invest. 2017;127:1353-1369 pubmed publisher
    ..Thus, we have revealed a signaling axis that is involved in the coordination of nutrient availability, protein acetylation, and cellular lipid metabolic responses. ..
  25. Thomas L, Makaroff A, Oldmeadow C, Attia J, Levi C. Seasonal variation in cervical artery dissection in the Hunter New England region, New South Wales, Australia: A retrospective cohort study. Musculoskelet Sci Pract. 2017;27:106-111 pubmed publisher
    ..7%; p = 0.014). A history of mild trauma was more common in VAD than internal carotid artery dissection (ICAD) (14, 41.2% vs 3, 13.0%; p = 0.023)...
  26. Lee S, Parks J, Kang H. Quercetin, a functional compound of onion peel, remodels white adipocytes to brown-like adipocytes. J Nutr Biochem. 2017;42:62-71 pubmed publisher
    ..This result indicates that onion peel has the potential to remodel the characteristics of white adipocytes to those of brown-like adipocytes. ..
  27. da Costa Krewer C, Santos Amanso E, Veneroni Gouveia G, de Lima Souza R, da Costa M, Aparecido Mota R. Resistance to antimicrobials and biofilm formation in Staphylococcus spp. isolated from bovine mastitis in the Northeast of Brazil. Trop Anim Health Prod. 2015;47:511-8 pubmed publisher
    ..7 and 96.3% of samples, respectively, on Congo red agar and on the microplate adhesion test, while the icaD gene was present in 92.2% of Staphylococcus spp...
  28. Rodor J, Pan Q, Blencowe B, Eyras E, Caceres J. The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation. RNA. 2016;22:1411-26 pubmed publisher
    ..In particular, Acinus regulates the splicing of DFFA/ICAD transcript, a major regulator of DNA fragmentation...
  29. Park M, Shin J, Lee S, Park H, Park K. RNF213 rs112735431 polymorphism in intracranial artery steno-occlusive disease and moyamoya disease in Koreans. J Neurol Sci. 2017;375:331-334 pubmed publisher
    ..disease (MMD), may be associated with non-MMD intracranial artery steno-occlusive disease of non-MMD type (non-MMD ICAD) in Asian...
  30. Li J, Liu G, Zhang F, Zhang Z, Xu Y, Li Q. Role of glycoprotein 78 and cidec in hepatic steatosis. Mol Med Rep. 2017;16:1871-1877 pubmed publisher
    ..Based on these data, it was hypothesized that gp78 is a regulator of hepatic steatosis, and that it may be a putative molecular mediator in metabolic diseases. ..
  31. Rahimi F, Katouli M, Karimi S. Biofilm production among methicillin resistant Staphylococcus aureus strains isolated from catheterized patients with urinary tract infection. Microb Pathog. 2016;98:69-76 pubmed publisher
    ..Eighty three (77%) strains were positive for biofilm formation and also carried icaA and icaD genes...
  32. Giannini N, Ulivi L, Maccarrone M, Montano V, Orlandi G, Ferrari E, et al. Epidemiology and cerebrovascular events related to cervical and intracranial arteries dissection: the experience of the city of Pisa. Neurol Sci. 2017;38:1985-1991 pubmed publisher
    ..7 to 3/100,000/year for extracranial internal carotid artery (ICAD) and 1 to 1.9/100,000/year for extracranial vertebral artery (VAD)...
  33. Morgan C, Diaz J, Zeitlin S, Gray D, Wells J. Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling. Proc Natl Acad Sci U S A. 2015;112:8344-9 pubmed publisher
    ..CAD is activated when caspases cleave its endogenous inhibitor ICAD, resulting in the characteristic DNA laddering of apoptosis...
  34. Agarwala M, Asad A, Gummadi N, Chidambaram S, Venkateswaralu J. Bilateral spontaneous internal carotid artery dissection managed with endovascular stenting - A case report. Indian Heart J. 2016;68 Suppl 2:S69-S71 pubmed publisher
    ..sup>4 Bilateral internal carotid artery dissection (ICAD) has been rarely reported.1,4 Antiplatelets and anticoagulation remain standard therapy for CAD...
  35. Kurre W, Bansemir K, Aguilar Perez M, Martinez Moreno R, Schmid E, Bazner H, et al. Endovascular treatment of acute internal carotid artery dissections: technical considerations, clinical and angiographic outcome. Neuroradiology. 2016;58:1167-1179 pubmed
    In acute internal carotid artery dissection (a-ICAD) with concomitant intracranial large vessel occlusion or haemodynamic impairment, the effectiveness of medical treatment is limited and endovascular therapy (EVT) can be considered...
  36. Sun J, Luo H, Nie W, Xu X, Miao X, Huang F, et al. Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL. Int J Clin Exp Pathol. 2015;8:6519-25 pubmed
    ..Western blot was applied to test c-FLIP, RIP, DR4, DR5, FADD, Caspase-3/8/9, ERK1/2, and DFF45 protein expression. Caspase-8 kit was used to detect Caspase-8 expression...
  37. Castilho I, Dantas S, Langoni H, Araújo J, Fernandes A, Alvarenga F, et al. Host-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis. J Dairy Sci. 2017;100:6414-6421 pubmed publisher
    ..collected from the milk of cows with subclinical mastitis that had 8 possible combinations of bap, icaA, and icaD genes, to determine their capacity to produce biofilm on biotic (bovine primary mammary epithelial cells and HeLa ..
  38. Xu M, Cai Y, Wang H, Altamirano J, Chang B, Bertola A, et al. Fat-Specific Protein 27/CIDEC Promotes Development of Alcoholic Steatohepatitis in Mice and Humans. Gastroenterology. 2015;149:1030-41.e6 pubmed publisher
    ..with ASH progression and focused on the mouse fat-specific protein 27 (FSP-27)/human cell death-inducing DFF45-like effector C (CIDEC) protein, which is expressed in white adipose tissues and promotes formation of fat ..
  39. Wu J, Jiang Y, Cao Z, Li Z, Hu Y, Li H, et al. Enhanced anaerobic digestion of waste activated sludge of low organic content in a novel digester. Water Sci Technol. 2015;72:966-73 pubmed publisher
    A novel digester, termed an internal circulation anaerobic digester (ICAD), was developed to intensify sludge digestion. It consists of reaction zone, settling zone, thickening zone, riser and downcomer...
  40. Toraih E, Aly N, Abdallah H, Al Qahtani S, Shaalan A, Hussein M, et al. MicroRNA-target cross-talks: Key players in glioblastoma multiforme. Tumour Biol. 2017;39:1010428317726842 pubmed publisher
    ..Future translational and functional studies are highly recommended to better understand the complex bio-molecular signature of this difficult-to-treat tumor...
  41. Osman K, Abd El Razik K, Marie H, Arafa A. Relevance of biofilm formation and virulence of different species of coagulase-negative staphylococci to public health. Eur J Clin Microbiol Infect Dis. 2015;34:2009-16 pubmed publisher
    ..slime production using the tube test (TA) and the Congo red agar plate test (CRA), while the presence of icaA and icaD genes were evaluated by d-PCR...
  42. Mei C, Gui L, Fu C, Wang H, Wang J, Cheng G, et al. Polymorphisms in the bovine CIDEC gene are associated with body measurement traits and meat quality traits in Qinchuan cattle. Genet Mol Res. 2015;14:9013-23 pubmed publisher
    Previous studies have shown that the cell death-inducing DFF45-like effector-C (CIDEC) gene is involved in lipid storage and energy metabolism, suggesting that it is a potential candidate gene that affects body measurement traits (BMTs) ..
  43. Yu G, Kwon S, Hwang J, An S, Park D, Kang D, et al. Effects of cell death-inducing DFF45-like effector B on meat quality traits in Berkshire pigs. Genet Mol Res. 2017;16: pubmed publisher
    Cell death-inducing DFF45-like effector (CIDE) B is a member of the CIDE family of apoptosis-inducing factors. In the present study, we detected a single nucleotide polymorphism (SNP), c...
  44. Sunatani Y, Kamdar R, Sharma M, Matsui T, Sakasai R, Hashimoto M, et al. Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase. Exp Cell Res. 2018;362:450-460 pubmed publisher
    ..CAD activity is reportedly regulated by the ratio of two inhibitor of CAD (ICAD) splice variants, ICAD-L and ICAD-S mRNA, which, respectively, produce proteins with and without the ..
  45. Al Kasab S, Derdeyn C, Guerrero W, Limaye K, Shaban A, Adams H. Intracranial Large and Medium Artery Atherosclerotic Disease and Stroke. J Stroke Cerebrovasc Dis. 2018;27:1723-1732 pubmed publisher
    Intracranial atherosclerotic disease (ICAD) is one of the most common causes of stroke worldwide and is associated with a high risk of stroke recurrence...
  46. Oh J, Grosshans D, Wong S, Slamon D. Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells. Nucleic Acids Res. 1999;27:4008-17 pubmed
    ..Further characterization of these genes may yield insight into the fundamental biology and pathogenetic effects of HER-2/ neu overexpression in human breast and ovarian cancer cells. ..
  47. Cai H, Yao W, Li L, Li X, Hu L, Mai R, et al. Cell-death-inducing DFFA-like Effector B Contributes to the Assembly of Hepatitis C Virus (HCV) Particles and Interacts with HCV NS5A. Sci Rep. 2016;6:27778 pubmed publisher
    ..Collectively, these results indicate that CIDEB is a new host factor that is involved in HCV assembly, presumably by interacting with viral protein, providing new insight into the exploitation of the VLDL regulator CIDEB by HCV. ..
  48. Martin J, de Oliveira E Silva G, da Fonseca C, Morales C, Souza Pamplona Silva C, Miquelluti D, et al. Efficiency of a cleaning protocol for the removal of enterotoxigenic Staphylococcus aureus strains in dairy plants. Int J Food Microbiol. 2016;238:295-301 pubmed publisher
    ..For genotypic analyses, the presence of icaA and icaD in strains was evaluated...
  49. Ocal D, Dolapci I, Karahan Z, Tekeli A. [Investigation of biofilm formation properties of staphylococcus isolates]. Mikrobiyol Bul. 2017;51:10-19 pubmed
    ..production of 166 staphylococci was phenotypically investigated on Congo Red Agar (CRA); the presence of icaA, icaD and IS256 genes were investigated by polymerase chain reaction (PCR). 74 of the isolates (44...
  50. Tan X, Cao Z, Li M, Xu E, Wang J, Xiao Y. TNF-? downregulates CIDEC via MEK/ERK pathway in human adipocytes. Obesity (Silver Spring). 2016;24:1070-80 pubmed publisher
    Cell death-inducing DFF45-like effector C (CIDEC) is a lipid droplet-coating protein that promotes triglyceride accumulation and inhibits lipolysis...
  51. McCarty J, Toh S, Li P. Multiple domains of DFF45 bind synergistically to DFF40: roles of caspase cleavage and sequestration of activator domain of DFF40. Biochem Biophys Res Commun. 1999;264:181-5 pubmed
    CAD/DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45/ICAD...
  52. Xerri L, Palmerini F, Devilard E, Defrance T, Bouabdallah R, Hassoun J, et al. Frequent nuclear localization of ICAD and cytoplasmic co-expression of caspase-8 and caspase-3 in human lymphomas. J Pathol. 2000;192:194-202 pubmed
    ..activates caspase-3, which in turn activates the caspase-activated DNAse (CAD) by proteolysis of its inhibitor (ICAD)...
  53. Dahal G, Karki P, Thapa A, Shahnawaz M, Shin S, Lee J, et al. Caspase-2 cleaves DNA fragmentation factor (DFF45)/inhibitor of caspase-activated DNase (ICAD). Arch Biochem Biophys. 2007;468:134-9 pubmed
    ..Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of ..
  54. Park H. Fifty C-terminal amino acid residues are necessary for the chaperone activity of DFF45 but not for the inhibition of DFF40. BMB Rep. 2009;42:713-8 pubmed
    ..DFF40 exists as a heterodimer with DFF45 (ICAD), which is a specific chaperone and inhibitor of DFF40 under normal conditions...
  55. Bagheri F, Safarian S, Eslaminejad M, Sheibani N. siRNA-mediated knock-down of DFF45 amplifies doxorubicin therapeutic effects in breast cancer cells. Cell Oncol (Dordr). 2013;36:515-26 pubmed publisher
    ..Here we examined the effects of siRNA-mediated DNA fragmentation factor 45 (DFF45) gene silencing on breast cancer cell viability, cell cycle arrest, and apoptosis in the presence and absence of ..
  56. Katsogiannou M, Andrieu C, Baylot V, Baudot A, Dusetti N, Gayet O, et al. The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets. Mol Cell Proteomics. 2014;13:3585-601 pubmed publisher
    ..Validation of Hsp27 involvement in both processes in human prostate cancer cells supports our system biology-predicted functions and provides new insights into Hsp27 roles in cancer cells. ..
  57. Yasumoto J, Kasai H, Yoshimura K, Otoguro T, Watashi K, Wakita T, et al. Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors. J Gen Virol. 2017;98:1762-1773 pubmed publisher
    ..Cell death-inducing DFF45-like effectors (CIDEs) B and C (CIDEB and CIDEC), which are involved in LD expansion for the improvement of lipid ..
  58. Tarp J, Bugge A, Andersen L, Sardinha L, Ekelund U, Brage S, et al. Does adiposity mediate the relationship between physical activity and biological risk factors in youth?: a cross-sectional study from the International Children's Accelerometry Database (ICAD). Int J Obes (Lond). 2017;: pubmed publisher
    ..To model the association between accumulating 60 daily minutes of moderate-to-vigorous physical activity and a composite score of biological risk factors into a direct and an indirect effect, using abdominal obesity as the mediator...
  59. Qiao Y, Suri F, Zhang Y, Liu L, Gottesman R, Alonso A, et al. Racial Differences in Prevalence and Risk for Intracranial Atherosclerosis in a US Community-Based Population. JAMA Cardiol. 2017;: pubmed publisher
    Intracranial atherosclerotic disease (ICAD) is an important cause of stroke; however, little is known about racial differences in ICAD prevalence and its risk factors.
  60. Jiang B, Yin S, You B, Gong Y, Huang G, Yang Z, et al. Antimicrobial resistance and virulence genes profiling of methicillin-resistant Staphylococcus aureus isolates in a burn center: A 5-year study. Microb Pathog. 2018;114:176-179 pubmed publisher
    ..showed that most of MRSA isolates in our center carried the virulence factor pattern of cna-clfA-clfB-eno-fib-icaA-icaD-sea-psm?-lukED-hlg-hlgv-hla-hld (214/239, 89.54%)...
  61. Todor A, Sharov V, Tanhehco E, Silverman N, Bernabei A, Sabbah H. Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes. Am J Physiol Heart Circ Physiol. 2002;283:H990-5 pubmed
    ..Expression of caspase-3 and DNA fragmentation factor-45 (DFF45)/inhibitor of caspase-3-activated DNase (ICAD) was detected by Western blot analysis...
  62. Nie Z, Phenix B, Lum J, Alam A, Lynch D, Beckett B, et al. HIV-1 protease processes procaspase 8 to cause mitochondrial release of cytochrome c, caspase cleavage and nuclear fragmentation. Cell Death Differ. 2002;9:1172-84 pubmed
    ..The effect of HIV-1 protease is not seen in T cell extracts which have undetectable levels of procaspase 8, indicating a specificity and requirement for procaspase 8. ..
  63. Korn C, Scholz S, Gimadutdinow O, Lurz R, Pingoud A, Meiss G. Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state. J Biol Chem. 2005;280:6005-15 pubmed
    DNA fragmentation factor (DFF) is a complex of the DNase DFF40 (CAD) and its chaperone/inhibitor DFF45 (ICAD-L) that can be activated during apoptosis to induce DNA fragmentation...
  64. Lu H, Hou Q, Zhao T, Zhang H, Zhang Q, Wu L, et al. Granzyme M directly cleaves inhibitor of caspase-activated DNase (CAD) to unleash CAD leading to DNA fragmentation. J Immunol. 2006;177:1171-8 pubmed
    ..Furthermore, GzmM cleaves the DNA damage sensor enzyme poly(ADP-ribose) polymerase to prevent cellular DNA repair and force apoptosis. ..
  65. Cullen S, Adrain C, Lüthi A, Duriez P, Martin S. Human and murine granzyme B exhibit divergent substrate preferences. J Cell Biol. 2007;176:435-44 pubmed
    ..GzmB exhibit radical differences in their ability to cleave BID, as well as several other key substrates, such as ICAD and caspase-8...
  66. Neimanis S, Albig W, Doenecke D, Kahle J. Sequence elements in both subunits of the DNA fragmentation factor are essential for its nuclear transport. J Biol Chem. 2007;282:35821-30 pubmed
    ..In proliferating cells DFF40 is expressed in the presence of its chaperone and inhibitor DFF45, which results in the formation of the DFF complex...
  67. Popiela T, Wicherek L, Radwan M, Sikora J, Banas T, Basta P, et al. The differences in RCAS1 and DFF45 endometrial expression between late proliferative, early secretory, and mid-secretory cycle phases. Folia Histochem Cytobiol. 2007;45 Suppl 1:S157-62 pubmed
    ..b>DFF45 seems to play an important role in the apoptotic process, most likely by acting through the regulation of DNA ..
  68. Omata K, Suzuki R, Masaki T, Miyamura T, Satoh T, Suzuki T. Identification and characterization of the human inhibitor of caspase-activated DNase gene promoter. Apoptosis. 2008;13:929-37 pubmed publisher
    DNA fragmentation factor is a heterodimer complex of the nuclease CAD and its specific inhibitor ICAD, which can be activated during apoptosis to induce DNA fragmentation...
  69. Walsh J, Cullen S, Sheridan C, Lüthi A, Gerner C, Martin S. Executioner caspase-3 and caspase-7 are functionally distinct proteases. Proc Natl Acad Sci U S A. 2008;105:12815-9 pubmed publisher
    ..Our observations provide a molecular basis for the different phenotypes seen in mice lacking either caspase and indicate that these proteases occupy nonredundant roles within the cell death machinery. ..
  70. Chetsawang B, Kooncumchoo P, Govitrapong P, Ebadi M. 1-Methyl-4-phenyl-pyridinium ion-induced oxidative stress, c-Jun phosphorylation and DNA fragmentation factor-45 cleavage in SK-N-SH cells are averted by selegiline. Neurochem Int. 2008;53:283-8 pubmed publisher
  71. Ninios Y, Sekeri Pataryas K, Sourlingas T. Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells. Apoptosis. 2010;15:128-38 pubmed publisher
    ..DFF is a complex of the endonuclease DFF40 and its chaperone/inhibitor, DFF45. In vitro work has shown that histone H1 and HMGB1/2 recruit/target DFF40 to the internucleosomal linker regions ..
  72. Zhang J, Guo H, Qian G, Ge S, Ji H, Hu X, et al. MiR-145, a new regulator of the DNA fragmentation factor-45 (DFF45)-mediated apoptotic network. Mol Cancer. 2010;9:211 pubmed publisher
    ..DNA Fragmentation Factor-45 (DFF45) has been found to be the substrate of Caspase-3, and the cleavage of DFF45 by caspase-3 during apoptosis releases ..
  73. Kutscher D, Pingoud A, Jeltsch A, Meiss G. Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase. FEBS J. 2012;279:2917-28 pubmed publisher
    ..fragmentation factor is a heterodimeric complex that consists of caspase-activated DNase (CAD) and its inhibitor (ICAD)...
  74. Errami Y, Brim H, Oumouna Benachour K, Oumouna M, Naura A, Kim H, et al. ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability. PLoS ONE. 2013;8:e57871 pubmed publisher
    ..showed that DNA fragmentation factor, which comprises a caspase-3-activated DNase (CAD) and its inhibitor (ICAD), may influence the rate of cell death by generating PARP-1-activating DNA breaks...
  75. Bazari P, Honarmand Jahromy S, Zare Karizi S. Phenotypic and genotypic characterization of biofilm formation among Staphylococcus aureus isolates from clinical specimens, an Atomic Force Microscopic (AFM) study. Microb Pathog. 2017;110:533-539 pubmed publisher
    ..The prevalence of icaA and icaD genes was determined by PCR. By CRA method 15% of samples considered as positive slime producers, 44...
  76. Torbus Paluszczak M, Mucha S, Wawrzyńczyk M, Pierzchała K, Bartman W, Adamczyk Sowa M. Hypoglossal nerve palsy in the course of dissection of the internal carotid arteries - Case reports. Neurol Neurochir Pol. 2018;52:267-273 pubmed publisher
    Internal carotid artery dissection (ICAD) has become an increasingly recognized cause of cerebrovascular accidents in young and middle-aged patients...
  77. Adepoju A, Adamo M. Posttraumatic complications in pediatric skull fracture: dural sinus thrombosis, arterial dissection, and cerebrospinal fluid leakage. J Neurosurg Pediatr. 2017;20:598-603 pubmed publisher
    ..study was to determine the rate of fracture associated with venous thrombosis, intracranial arterial dissection (ICAD), and cerebrospinal fluid (CSF) leakage in pediatric patients...
  78. Banas T, Pitynski K, Okon K, Czerw A. DNA fragmentation factors 40 and 45 (DFF40/DFF45) and B-cell lymphoma 2 (Bcl-2) protein are underexpressed in uterine leiomyosarcomas and may predict survival. Onco Targets Ther. 2017;10:4579-4589 pubmed publisher
    DNA fragmentation factors 40 and 45 (DFF40 and DFF45) are responsible for final DNA-laddering during apoptosis, whereas Bcl-2 (B-cell lymphoma 2) is an apoptosis inhibitor...
  79. Sui D, Zhou H, Wang F, Zhong M, Zhang W, Ti Y. CIDEC Gene Silencing Alleviates Pulmonary Vascular Remodeling in a Type 2 Diabetic Rat Model. J Diabetes Investig. 2017;: pubmed publisher
    ..We aimed to investigate whether the CIDEC gene silencing could alleviate pulmonary vascular remodeling in a type 2 diabetic rat model...
  80. Esianor B, Haider A, Engelhardt M, Osumah T, Vayalumkal S, Thakur R, et al. Intracranial Ischemic Infarct Due to Blunt Force Trauma in a High School Football Player. Cureus. 2017;9:e1659 pubmed publisher
    ..Here, we present the case of a 16-year-old male who developed internal carotid artery dissection (ICAD) after a head injury and subsequently developed ischemic stroke and seizures.
  81. Kim C, Jeon S, Choi J, Lee J, Park H. Interaction mode of CIDE family proteins in fly: DREP1 and DREP3 acidic surfaces interact with DREP2 and DREP4 basic surfaces. PLoS ONE. 2017;12:e0189819 pubmed publisher
    Cell death-inducing DNA fragmentation factor 45 (DFF45)-like effector (CIDE) domains were initially identified as protein interaction modules in apoptotic nucleases and are now known to form a highly conserved family with diverse ..
  82. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 1998;17:2526-33 pubmed
    b>DFF45 is a subunit of the DNA fragmentation factor (DFF) that is cleaved by caspase-3 during apoptosis. However, the mechanism by which DFF45 regulates apoptotic cell death remains poorly understood...
  83. McCarty J, Toh S, Li P. Study of DFF45 in its role of chaperone and inhibitor: two independent inhibitory domains of DFF40 nuclease activity. Biochem Biophys Res Commun. 1999;264:176-80 pubmed
    CAD/DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45/ICAD...
  84. Nakanuma Y, Tsuneyama K, Sasaki M, Harada K. Destruction of bile ducts in primary biliary cirrhosis. Baillieres Best Pract Res Clin Gastroenterol. 2000;14:549-70 pubmed
    ..Multiple factors, particularly the Fas system, are stimuli of apoptosis. Anoikis with decreased biliary expression of integrin 6, a ligand for laminin, may also be involved in biliary epithelial apoptosis. ..
  85. Zhou P, Lugovskoy A, McCarty J, Li P, Wagner G. Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45. Proc Natl Acad Sci U S A. 2001;98:6051-5 pubmed
    ..Expression and folding of DFF40 require the presence of DFF45, which also acts as a nuclease inhibitor before DFF40 activation by execution caspases...
  86. Tsukada T, Watanabe M, Yamashima T. Implications of CAD and DNase II in ischemic neuronal necrosis specific for the primate hippocampus. J Neurochem. 2001;79:1196-206 pubmed
    ..The interactions between apoptotic (caspase-3 and CAD) and necrotic (calpain, cathepsin and DNase II) cascades should be studied further. ..
  87. Fukushima K, Kikuchi J, Koshiba S, Kigawa T, Kuroda Y, Yokoyama S. Solution structure of the DFF-C domain of DFF45/ICAD. A structural basis for the regulation of apoptotic DNA fragmentation. J Mol Biol. 2002;321:317-27 pubmed
    b>DFF45/ICAD has dual functions in the final stage of apoptosis, by acting as both a folding chaperone and a DNase inhibitor of DFF40/CAD...
  88. Widlak P, Lanuszewska J, Cary R, Garrard W. Subunit structures and stoichiometries of human DNA fragmentation factor proteins before and after induction of apoptosis. J Biol Chem. 2003;278:26915-22 pubmed
    ..the expression of DFF subunits varied widely among the cell types studied, and the chaperone/inhibitor subunits DFF45 and DFF35 unexpectedly existed primarily as monomers in vast excess of the latent nuclease subunit, DFF40, which ..
  89. Pérez González A, Rodriguez A, Huarte M, Salanueva I, Nieto A. hCLE/CGI-99, a human protein that interacts with the influenza virus polymerase, is a mRNA transcription modulator. J Mol Biol. 2006;362:887-900 pubmed
    ..Collectively these results indicate that hCLE works as a positive modulator of the RNA polymerase II activity. ..
  90. Iglesias Guimarais V, Gil Guiñón E, Gabernet G, García Belinchón M, Sánchez Osuna M, Casanelles E, et al. Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease. J Biol Chem. 2012;287:7766-79 pubmed publisher
    ..The endogenous inhibitor of DFF40/CAD, ICAD, is correctly processed, and dff40/cad cDNA sequence does not reveal mutations altering its amino acid composition...
  91. Banas T, Basta P, Knafel A, Skotniczny K, Jach R, Hajdyla Banaś I, et al. DFF45 expression in human endometrium is associated with menstrual cycle phases and decreases after menopause. Gynecol Obstet Invest. 2012;73:177-82 pubmed publisher
    ..and if no conception occurs menstruation is observed as the result of endometrial cell apoptosis via DFF40/DFF45 complex activation...
  92. Leu S, Lin Y, Wu C, Ouyang P. Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD. J Cell Sci. 2012;125:3164-72 pubmed publisher
    ..RT-PCR analysis showed that alternative splicing patterns of SRSF1 as well as of apoptosis-related genes Bcl-x and ICAD were altered, and expression levels of Bim isoforms were modulated in Pnn-depleted cells...