DDX6

Summary

Gene Symbol: DDX6
Description: DEAD-box helicase 6
Alias: HLR2, P54, RCK, ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box helicase 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), oncogene RCK
Species: human
Products:     DDX6

Top Publications

  1. Lin F, Wang R, Shen J, Wang X, Gao P, Dong K, et al. Knockdown of RCK/p54 expression by RNAi inhibits proliferation of human colorectal cancer cells in vitro and in vivo. Cancer Biol Ther. 2008;7:1669-76 pubmed
    ..The overexpression of RCK/p54 gene, a member of the DEAD box protein/RNA helicase family, has been found in this malignancy...
  2. Ernoult Lange M, Baconnais S, Harper M, Minshall N, Souquere S, Boudier T, et al. Multiple binding of repressed mRNAs by the P-body protein Rck/p54. RNA. 2012;18:1702-15 pubmed publisher
    ..We demonstrate here that the human DEAD-box protein Rck/p54, which is a component of such complexes and central to P-body assembly, is in considerable molecular excess with ..
  3. de Vries S, Naarmann de Vries I, Urlaub H, Lue H, Bernhagen J, Ostareck D, et al. Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia. J Biol Chem. 2013;288:5815-27 pubmed publisher
    ..Interestingly, mass spectrometry analysis identified the DEAD-box RNA helicase 6 (DDX6) that interacts with the VEGF mRNA 5'-UTR...
  4. Tritschler F, Braun J, Motz C, Igreja C, Haas G, Truffault V, et al. DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa. Proc Natl Acad Sci U S A. 2009;106:21591-6 pubmed publisher
    ..Our results reveal an unexpected connectivity and complexity of the mRNA decapping network in multicellular eukaryotes, which likely enhances opportunities for regulating mRNA degradation. ..
  5. Nathans R, Chu C, Serquina A, Lu C, Cao H, Rana T. Cellular microRNA and P bodies modulate host-HIV-1 interactions. Mol Cell. 2009;34:696-709 pubmed publisher
    ..Thus we provide an example of a single host miRNA regulating HIV-1 production and infectivity. These studies highlight the significance of miRNAs and P bodies in modulating host cell interactions with HIV-1 and possibly other viruses. ..
  6. Tritschler F, Braun J, Eulalio A, Truffault V, Izaurralde E, Weichenrieder O. Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B. Mol Cell. 2009;33:661-8 pubmed publisher
    The DEAD box helicase DDX6/Me31B functions in translational repression and mRNA decapping. How particular RNA helicases are recruited specifically to distinct functional complexes is poorly understood...
  7. Chen Y, Boland A, Kuzuoğlu Öztürk D, Bawankar P, Loh B, Chang C, et al. A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Mol Cell. 2014;54:737-50 pubmed publisher
    ..We further show that the CNOT1 MIF4G domain interacts with the C-terminal RecA domain of DDX6, a translational repressor and decapping activator...
  8. Chu C, Rana T. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol. 2006;4:e210 pubmed
    ..Here, we show that RCK/p54, a DEAD box helicase, interacts with argonaute proteins, Ago1 and Ago2, in affinity-purified active siRISC or ..
  9. Matsui T, Hogetsu K, Usukura J, Sato T, Kumasaka T, Akao Y, et al. Structural insight of human DEAD-box protein rck/p54 into its substrate recognition with conformational changes. Genes Cells. 2006;11:439-52 pubmed
    Human rck/p54, a product of the gene cloned at the breakpoint of t(11; 14) (q23;q32) chromosomal translocation on 11q23 in B-cell lymphoma, is a member of the DEAD-box RNA helicase family...

More Information

Publications92

  1. Rouya C, Siddiqui N, Morita M, Duchaine T, Fabian M, Sonenberg N. Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1. RNA. 2014;20:1398-409 pubmed publisher
    ..the large scaffolding subunit of CCR4-NOT, CNOT1, with the translational repressor and decapping activator protein, DDX6. DDX6 binds to a conserved CNOT1 subdomain in a manner resembling the interaction of the translation initiation ..
  2. Akao Y, Marukawa O, Morikawa H, Nakao K, Kamei M, Hachiya T, et al. The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues. Cancer Res. 1995;55:3444-9 pubmed
    ..Two different polyclonal anti-rck antibodies detected a specific 54-kilodalton product named rck/p54 in the majority of human and mouse tissues tested by Western blot analysis...
  3. Smillie D, Sommerville J. RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles. J Cell Sci. 2002;115:395-407 pubmed
    ..Xp54 belongs to small family of helicases (DDX6) that associate with mRNA molecules encoding proteins required for progress through meiosis...
  4. Nakagawa Y, Morikawa H, Hirata I, Shiozaki M, Matsumoto A, Maemura K, et al. Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours. Br J Cancer. 1999;80:914-7 pubmed
    ..chromosomal translocation observed in human B-cell lymphoma, and the overexpression of its protein (rck/p54) by the translocation was shown to cause malignant transformation...
  5. Fenger Grøn M, Fillman C, Norrild B, Lykke Andersen J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Mol Cell. 2005;20:905-15 pubmed
    ..Here, we identify three proteins that exist in complex with the decapping enzyme subunits hDcp2 and hDcp1: hEdc3, Rck/p54, and a protein in decapping we name Hedls...
  6. Phalora P, Sherer N, Wolinsky S, Swanson C, Malim M. HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies. J Virol. 2012;86:11712-24 pubmed publisher
    ..between APOBEC3 protein incorporation into virions and localization to P bodies, depletion of the P-body components DDX6 or Lsm1 did not affect HIV-1 replication, APOBEC3 packaging into virions or APOBEC3 protein mediated inhibition of ..
  7. Ozgur S, Chekulaeva M, Stoecklin G. Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies. Mol Cell Biol. 2010;30:4308-23 pubmed publisher
    ..In addition, the RNA helicase Rck and Lsm1 proteins interact with human Pat1b...
  8. Jangra R, Yi M, Lemon S. DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation. J Virol. 2010;84:6810-24 pubmed publisher
    b>DDX6 (Rck/p54) is an evolutionarily conserved member of the SF2 DEAD-box RNA helicase family that contributes to the regulation of translation and storage and the degradation of cellular mRNAs...
  9. Reed J, Molter B, Geary C, McNevin J, McElrath J, Giri S, et al. HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. J Cell Biol. 2012;198:439-56 pubmed publisher
    ..contain endogenous processing body (PB)-related proteins, including AGO2 and the ribonucleic acid (RNA) helicase DDX6. Moreover, we found a similar complex containing ABCE1 and PB proteins in uninfected cells...
  10. Naarmann I, Harnisch C, Muller Newen G, Urlaub H, Ostareck Lederer A, Ostareck D. DDX6 recruits translational silenced human reticulocyte 15-lipoxygenase mRNA to RNP granules. RNA. 2010;16:2189-204 pubmed publisher
    ..the DICE as bait combined with hnRNP K immunoprecipitation, we specifically purified the DEAD-box RNA helicase 6 (DDX6) that interacts with hnRNP K and hnRNP E1 in a DICE-dependent manner...
  11. Minshall N, Kress M, Weil D, Standart N. Role of p54 RNA helicase activity and its C-terminal domain in translational repression, P-body localization and assembly. Mol Biol Cell. 2009;20:2464-72 pubmed publisher
    The RNA helicase p54 (DDX6, Dhh1, Me31B, Cgh-1, RCK) is a prototypic component of P-(rocessing) bodies in cells ranging from yeast to human...
  12. Nonhoff U, Ralser M, Welzel F, Piccini I, Balzereit D, Yaspo M, et al. Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules. Mol Biol Cell. 2007;18:1385-96 pubmed
    ..a function of ataxin-2 in such pathways by demonstrating that ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6, a component of P-bodies and stress granules, representing cellular structures of mRNA triage...
  13. Miyaji K, Nakagawa Y, Matsumoto K, Yoshida H, Morikawa H, Hongou Y, et al. Overexpression of a DEAD box/RNA helicase protein, rck/p54, in human hepatocytes from patients with hepatitis C virus-related chronic hepatitis and its implication in hepatocellular carcinogenesis. J Viral Hepat. 2003;10:241-8 pubmed
    ..The RCK protein, rck/p54, which is a 54-kDa cytoplasmic protein belonging to the DEAD box/RNA helicase family, is considered to facilitate ..
  14. Wichroski M, Robb G, Rana T. Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. PLoS Pathog. 2006;2:e41 pubmed
    ..which have established roles in cap-dependent translation (eIF4E and eIF4E-T), translation suppression (RCK/p54), RNA interference-mediated post-transcriptional gene silencing (AGO2), and decapping of mRNA (DCP2)...
  15. Ward A, Bidet K, Yinglin A, Ler S, Hogue K, Blackstock W, et al. Quantitative mass spectrometry of DENV-2 RNA-interacting proteins reveals that the DEAD-box RNA helicase DDX6 binds the DB1 and DB2 3' UTR structures. RNA Biol. 2011;8:1173-86 pubmed publisher
    ..We identified several proteins, including DDX6, G3BP1, G3BP2, Caprin1, and USP10, implicated in P body (PB) and stress granule (SG) function, and not previously ..
  16. Yu S, Lujan P, Jackson D, Emerman M, Linial M. The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome. PLoS Pathog. 2011;7:e1002303 pubmed publisher
    ..Here we find that the cellular DEAD-box RNA helicase DDX6 is required for efficient genome packaging of foamy virus, a spumaretrovirus...
  17. Iio A, Takagi T, Miki K, Naoe T, Nakayama A, Akao Y. DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells. Biochim Biophys Acta. 2013;1829:1102-10 pubmed publisher
    ..Here, we show that DEAD-box RNA helicase 6, DDX6 (p54/RCK), post-transcriptionally down-regulated miR-143/145 expression by prompting the degradation of its host ..
  18. Yurchenko M, Kovalevska L, Shlapatska L, Berdova G, Clark E, Sidorenko S. CD150 regulates JNK1/2 activation in normal and Hodgkin's lymphoma B cells. Immunol Cell Biol. 2010;88:565-74 pubmed publisher
    ..CD150 mediated activation of JNK1/2 p54 and JNK2-gamma kinase isoforms in all CD150(+) B cell lines we tested...
  19. Kahl A, Blanco I, Jackman K, Baskar J, Milaganur Mohan H, Rodney Sandy R, et al. Cerebral ischemia induces the aggregation of proteins linked to neurodegenerative diseases. Sci Rep. 2018;8:2701 pubmed publisher
    ..These include TDP43, FUS, hnRNPA1, PSF/SFPQ and p54/NONO, all of which have been linked to neurodegeneration associated with amyotrophic lateral sclerosis and ..
  20. Alessi A, Khivansara V, Han T, Freeberg M, Moresco J, Tu P, et al. Casein kinase II promotes target silencing by miRISC through direct phosphorylation of the DEAD-box RNA helicase CGH-1. Proc Natl Acad Sci U S A. 2015;112:E7213-22 pubmed publisher
    ..Importantly, we identify the conserved DEAD-box RNA helicase, CGH-1/DDX6, as a key CK2 substrate within miRISC and demonstrate phosphorylation of a conserved N-terminal serine is required ..
  21. Zhang X, Lui W. Transforming growth factor-β3 regulates cell junction restructuring via MAPK-mediated mRNA destabilization and Smad-dependent protein degradation of junctional adhesion molecule B (JAM-B). Biochim Biophys Acta. 2015;1849:601-11 pubmed publisher
    ..degradation of JAM-B transcript and TGF-β3-mediated mRNA destabilization requires the activation of ERK1/2 and p54 JNK signal cascades...
  22. Le Niculescu H, ROSEBERRY K, Levey D, Rogers J, Kosary K, Prabha S, et al. Towards precision medicine for stress disorders: diagnostic biomarkers and targeted drugs. Mol Psychiatry. 2019;: pubmed publisher
    ..the biomarkers with the best overall convergent functional evidence (CFE) for involvement in stress were FKBP5, DDX6, B2M, LAIR1, RTN4, and NUB1...
  23. Lopes G, Michael G, Cardoso M, Schwarz S. Antimicrobial resistance and class 1 integron-associated gene cassettes in Salmonella enterica serovar Typhimurium isolated from pigs at slaughter and abattoir environment. Vet Microbiol. 2016;194:84-92 pubmed publisher
    ..Salmonella plasmid-located virulence genes spvR, spvA, spvB, rck and pefA were found on an IncFIB resistance plasmid...
  24. Birhanu B, Park N, Lee S, Hossain M, Park S. Inhibition of Salmonella Typhimurium adhesion, invasion, and intracellular survival via treatment with methyl gallate alone and in combination with marbofloxacin. Vet Res. 2018;49:101 pubmed publisher
    ..Quorum sensing genes including sdiA, srgE, and rck were downregulated by 52.8%, 61.7%, and 22.2%, respectively. Moreover, rac-1 was downregulated by 56.9% and 71...
  25. Paul J, Naskar K, Chowdhury S, Alam M, Chakraborti T, De T. TLR4-mediated activation of MyD88 signaling induces protective immune response and IL-10 down-regulation in Leishmania donovani infection. Indian J Biochem Biophys. 2014;51:531-41 pubmed
    ..the degradation of IKB and the nuclear translocation of NF-κB, as well as rapid phosphorylation of p38 MAPK and p54/56 JNK. Knockdown of TLR4 or MYD88 using siRNA showed reduced inflammatory response to GP29 in LD-infected cells...
  26. Renda A, Penty N, Komal P, Nashmi R. Vulnerability to nicotine self-administration in adolescent mice correlates with age-specific expression of ?4* nicotinic receptors. Neuropharmacology. 2016;108:49-59 pubmed publisher
    ..Nicotine self-administration was elevated in the P44 group, peaked at P54-60 and was drastically lower in the P66 through P86 groups...
  27. Kubasova T, Matiasovicova J, Rychlik I, Juricova H. Complete sequence of multidrug resistance p9134 plasmid and its variants including natural recombinant with the virulence plasmid of Salmonella serovar Typhimurium. Plasmid. 2014;76:8-14 pubmed publisher
    ..During the first one, the tetracycline resistance locus was replaced with rck, srgB, srgA, orf7 and pefI originating from Salmonella virulence plasmid pSLT...
  28. Mulumba Mfumu L, Achenbach J, Mauldin M, Dixon L, Tshilenge C, Thiry E, et al. Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants. Viruses. 2017;9: pubmed publisher
    ..gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32...
  29. James V, Zhang Y, Foxler D, de Moor C, Kong Y, Webb T, et al. LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proc Natl Acad Sci U S A. 2010;107:12499-504 pubmed publisher
    ..Furthermore, we reveal that LIMD1, Ajuba, and WTIP bind to Ago1/2, RCK, Dcp2, and eIF4E in vivo, that they are required for miRNA-mediated, but not siRNA-mediated gene silencing and that ..
  30. Semple S, Eshaque S, Fujiki K, Tang C, Mitchell L, Bols N, et al. CK-2 of rainbow trout (Oncorhynchus mykiss) has two differentially regulated alleles that encode a functional chemokine. Vet Immunol Immunopathol. 2018;198:26-36 pubmed publisher
    ..Upon the development of a polyclonal antibody to rCK-2, CK-2 was only observed in the brain, liver and head kidney of PHA stimulated rainbow trout tissues...
  31. Braun J, Tritschler F, Haas G, Igreja C, Truffault V, Weichenrieder O, et al. The C-terminal alpha-alpha superhelix of Pat is required for mRNA decapping in metazoa. EMBO J. 2010;29:2368-80 pubmed publisher
  32. Zhao S, Fang F, Tang X, Dou J, Wang W, Zheng X, et al. An in-depth analysis identifies two new independent signals in 11q23.3 associated with vitiligo in the Chinese Han population. J Dermatol Sci. 2017;88:103-109 pubmed publisher
    ..90×10-4, respectively). The C allele of rs638893 (a previously reported one) located upstream of DDX6 was also significantly associated with vitiligo (OR=1.25, 95% CI: 1.12-1.38, P=3.04×10-5)...
  33. Jung D, Ahn J, Rhee B, Kim J. Mutational analysis of the RNA helicase Dhh1 in Ste12 expression and yeast mating. J Microbiol. 2017;55:373-378 pubmed publisher
    Dhh1 and Dhh1 homologues (RCK/p54/DDX6) are members of the DEAD-box protein family of RNA helicases. These proteins display conserved sequence motifs for ATPase and RNA binding activities...
  34. Udayakumar D, Dynan W. Characterization of DNA binding and pairing activities associated with the native SFPQ·NONO DNA repair protein complex. Biochem Biophys Res Commun. 2015;463:473-8 pubmed publisher
    ..We have previously shown that a complex of SFPQ (PSF) and NONO (p54(nrb)) cooperates with Ku protein at an early step of NHEJ, forming a committed preligation complex and stimulating ..
  35. Bridge K, Shah K, Li Y, Foxler D, Wong S, Miller D, et al. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell Rep. 2017;20:173-187 pubmed publisher
    ..Ser 387 by Akt3 induces LIMD1 binding, which in turn enables AGO2 to interact with TNRC6A and downstream effector DDX6. Conservation of this serine in AGO1 and 4 indicates this mechanism may be a fundamental requirement for AGO ..
  36. Ho T, Huang J, Zhou N, Zhang Z, Koirala P, Zhou X, et al. Regulation of PCGEM1 by p54/nrb in prostate cancer. Sci Rep. 2016;6:34529 pubmed publisher
    ..In the present study, we show transcriptional regulation of PCGEM1 in response to androgen deprivation by p54/nrb. While ectopic expression of p54/nrb increases, suppression of p54/nrb by RNAi or knockout (KO) reduces PCGEM1...
  37. Sharma A, Shrivastava B, Kuhad R. Reduced toxicity of malachite green decolorized by laccase produced from Ganoderma sp. rckk-02 under solid-state fermentation. 3 Biotech. 2015;5:621-631 pubmed publisher
    ..Crinipellis sp. RCK-1, Ganoderma sp...
  38. Qi M, Wang Z, Zhang Z, Shao Q, Zeng A, Li X, et al. AU-rich-element-dependent translation repression requires the cooperation of tristetraprolin and RCK/P54. Mol Cell Biol. 2012;32:913-28 pubmed publisher
    ..Finally, we demonstrate that the general translational repressor RCK may cooperate with TTP to regulate ARE-mRNA translation...
  39. Zhang J, Zhang Y, Yang J, Zhang L, Sun L, Pan H, et al. Three SNPs in chromosome 11q23.3 are independently associated with systemic lupus erythematosus in Asians. Hum Mol Genet. 2014;23:524-33 pubmed publisher
    ..3 region, including variants in/near PHLDB1 (rs11603023, P(_combined) = 1.25E-08, OR = 1.20), DDX6 (rs638893, P(_combined) = 5.19E-07, OR = 1.22) and CXCR5 (rs10892301, P(_combined) = 2.51E-08, OR = 0.85)...
  40. Lopera Madrid J, Osorio J, He Y, Xiang Z, Adams L, Laughlin R, et al. Safety and immunogenicity of mammalian cell derived and Modified Vaccinia Ankara vectored African swine fever subunit antigens in swine. Vet Immunol Immunopathol. 2017;185:20-33 pubmed publisher
    ..Three HEK-purified antigens [B646L (p72), E183L (p54), and O61R (p12)], and three MVA-vectored antigens [B646L, EP153R, and EP402R (CD2v)] were evaluated using a prime-..
  41. Maldarelli F, Wu X, Su L, Simonetti F, Shao W, Hill S, et al. HIV latency. Specific HIV integration sites are linked to clonal expansion and persistence of infected cells. Science. 2014;345:179-83 pubmed publisher
    ..Our findings show that HIV integration sites can play a critical role in expansion and persistence of HIV-infected cells. ..
  42. Luykx J, Olde Loohuis L, Neeleman M, Strengman E, Bakker S, Lentjes E, et al. Peripheral blood gene expression profiles linked to monoamine metabolite levels in cerebrospinal fluid. Transl Psychiatry. 2016;6:e983 pubmed publisher
    ..8 × 10-8, ?=0.08) and DDX6 (P=2.9 × 10-7, ?=0.07)...
  43. Mugabo Y, Sadeghi M, Fang N, Mayor T, Lim G. Elucidation of the 14-3-3ζ interactome reveals critical roles of RNA-splicing factors during adipogenesis. J Biol Chem. 2018;293:6736-6750 pubmed publisher
    ..siRNA-mediated depletion of RNA-splicing factors within the 14-3-3ζ interactome, that is, of Hnrpf, Hnrpk, Ddx6, and Sfpq, revealed that they have essential roles in adipogenesis and in the alternative splicing of Pparg ..
  44. Chou S, Lai J, Cho C, Lee C. Relationships between surface roughness/stiffness of chitosan coatings and fabrication of corneal keratocyte spheroids: Effect of degree of deacetylation. Colloids Surf B Biointerfaces. 2016;142:105-113 pubmed publisher
    ..g., within 8h). With these stimulations from the substrate interfaces, the size and morphology of RCK spheroids were greatly affected by the DD of chitosan...
  45. Lee J, Yoo E, Lee H, Park K, Hur J, Lim C. LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker Neurons. Mol Cell. 2017;66:129-140.e7 pubmed publisher
    ..Here we employ the posttranscriptional co-activator function of Drosophila ATX2 to demonstrate that LSM12 and ME31B/DDX6 are two ATX2-associating factors crucial for sustaining circadian rhythms...
  46. Cho J, Kim S, Seo Y, Park S, Park B, Kim J, et al. The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3. J Biol Chem. 2017;292:11804-11814 pubmed publisher
    ..in miRNA silencing by recruiting downstream effectors, such as the translation regulator DEAD-box helicase 6 (DDX6) and transducer of ERBB2,1/2,2 (Tob1/2), to the Ago-GW182 complex...
  47. Ainaoui N, Hantelys F, Renaud Gabardos E, Bunel M, Lopez F, Pujol F, et al. Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation. PLoS ONE. 2015;10:e0136466 pubmed publisher
    ..Accordingly, knockdown of either p54 or hnRNPM also blocks endogenous FGF1 induction and myotube formation, demonstrating the physiological relevance of ..
  48. Nishimura T, Padamsi Z, Fakim H, Milette S, Dunham W, Gingras A, et al. The eIF4E-Binding Protein 4E-T Is a Component of the mRNA Decay Machinery that Bridges the 5' and 3' Termini of Target mRNAs. Cell Rep. 2015;11:1425-36 pubmed publisher
    ..Here, we show that 4E-T is a component of the mRNA decay machinery and interacts with factors including DDX6, LSM14, and the LSM1-7-PAT1 complex...
  49. Singh B, Krawetz M, De Lima R, Mukherjee R, Chaturvedi P, Lee Chan E, et al. Role of TGF-? in Self-Peptide Regulation of Autoimmunity. Arch Immunol Ther Exp (Warsz). 2018;66:11-19 pubmed publisher
    ..Immunization of NOD mice with the self-MHC class II I-Ag7 ?-chain-derived peptide, I-A?g7 p54-76 also induced large amounts of TGF-? and also protected these mice from diabetes development...
  50. Marnef A, Maldonado M, Bugaut A, Balasubramanian S, Kress M, Weil D, et al. Distinct functions of maternal and somatic Pat1 protein paralogs. RNA. 2010;16:2094-107 pubmed publisher
    ..Pat1a is a translational repressor in oocytes in a CPEB-containing complex, and Pat1b is a component of P-bodies in somatic cells...
  51. Duvignaud J, Bédard M, Nagata T, Muto Y, Yokoyama S, Gagné S, et al. Structure, Dynamics, and Interaction of p54(nrb)/NonO RRM1 with 5' Splice Site RNA Sequence. Biochemistry. 2016;55:2553-66 pubmed publisher
    b>p54(nrb)/NonO is a nuclear RNA-binding protein involved in many cellular events such as pre-mRNA processing, transcription, and nuclear retention of hyper-edited RNAs...
  52. Kim T, Kang Y, Park Z, Kim Y, Hong S, Oh S, et al. SH3RF2 functions as an oncogene by mediating PAK4 protein stability. Carcinogenesis. 2014;35:624-34 pubmed publisher
    ..Taken together, our results suggest that SH3RF2 is an oncogene that may be a definitive regulator of PAK4. Therefore, SH3RF2 may represent an effective therapeutic target for cancer treatment. ..
  53. Bish R, Cuevas Polo N, Cheng Z, Hambardzumyan D, Munschauer M, Landthaler M, et al. Comprehensive Protein Interactome Analysis of a Key RNA Helicase: Detection of Novel Stress Granule Proteins. Biomolecules. 2015;5:1441-66 pubmed publisher
    b>DDX6 (p54/RCK) is a human RNA helicase with central roles in mRNA decay and translation repression...
  54. Matsui T, Hogetsu K, Akao Y, Tanaka M, Sato T, Kumasaka T, et al. Crystallization and X-ray analysis of the N-terminal core domain of a tumour-associated human DEAD-box RNA helicase, rck/p54. Acta Crystallogr D Biol Crystallogr. 2004;60:156-9 pubmed
    The RCK gene was cloned on the basis of the t(11;14)(q23;q32) chromosome translocation observed in human B-cell lymphoma cell line RC-K8. This gene was found to be overexpressed in various kinds of tumours...
  55. Akiyama K, Akao Y, Yokoyama M, Nakagawa Y, Noguchi T, Yagi K, et al. Expression of two dead box genes (DDX1 and DDX6) is independent of that of MYCN in human neuroblastoma cell lines. Biochem Mol Biol Int. 1999;47:563-8 pubmed
    To examine whether two DEAD box genes, DDX1 and DDX6, would have some roles in the progression of tumors, we investigated the correlation of the expression of these genes with that of MYCN in neuroblastomas either with or without MYCN ..
  56. Biegel J, Henderson E, Cox E, Bonenfant G, Netzband R, Kahn S, et al. Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5' untranslated region of hepatitis C virus RNA. Virology. 2017;507:231-241 pubmed publisher
    Hepatitis C virus (HCV) subverts the cellular DEAD-box RNA helicase DDX6 to promote virus infection...
  57. Robinson B, Reed J, Geary C, Swain J, Lingappa J. A temporospatial map that defines specific steps at which critical surfaces in the Gag MA and CA domains act during immature HIV-1 capsid assembly in cells. J Virol. 2014;88:5718-41 pubmed publisher
    ..that all four critical surfaces act while Gag is associated with the cellular facilitators of assembly ABCE1 and DDX6. When correlated with existing structural data, our findings suggest the following model: Gag dimerizes via the CA-..
  58. Mathys H, Basquin J, Ozgur S, Czarnocki Cieciura M, Bonneau F, Aartse A, et al. Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression. Mol Cell. 2014;54:751-65 pubmed publisher
    ..Another domain of CNOT1 with an MIF4G fold recruits the DEAD-box ATPase DDX6, a known translational inhibitor...
  59. Chauhan D, Swain D, Shah N, Yadav H, Sharma A, Yadav B, et al. Modulation of voltage-gated sodium channels induces capacitation in bull spermatozoa through phosphorylation of tyrosine containing proteins. Theriogenology. 2018;108:207-216 pubmed publisher
    ..The immune blot study revealed the presence of five tyrosine phosphorylated proteins, namely-p17, p30, p54, p90 and p100...
  60. Broytman O, Westmark P, Gurel Z, Malter J. Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines. Neurobiol Aging. 2009;30:1962-74 pubmed publisher
    ..We also present evidence that at least one of these proteins, the DEAD-box helicase rck/p54, is involved in post-transcriptional regulation, as its overexpression in cultured cells results in elevated ..
  61. Rajgor D, Mellad J, Soong D, Rattner J, Fritzler M, Shanahan C. Mammalian microtubule P-body dynamics are mediated by nesprin-1. J Cell Biol. 2014;205:457-75 pubmed publisher
    ..Overexpression of dominant-negative p50(Nesp1) caused Rck/p54, but not GW182, displacement from microtubules, resulting in reduced PB movement and cross talk with stress ..
  62. Sharif H, Ozgur S, Sharma K, Basquin C, Urlaub H, Conti E. Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions. Nucleic Acids Res. 2013;41:8377-90 pubmed publisher
    ..The results suggest a model for how Dhh1-containing messenger ribonucleoprotein particles might be remodeled upon Pat1 and Edc3 binding. ..
  63. Huys A, Thibault P, Wilson J. Modulation of hepatitis C virus RNA accumulation and translation by DDX6 and miR-122 are mediated by separate mechanisms. PLoS ONE. 2013;8:e67437 pubmed publisher
    b>DDX6 and other P-body proteins are required for efficient replication of Hepatitis C Virus (HCV) by unknown mechanisms...
  64. Stary S, Vinatzer U, Müllauer L, Raderer M, Birner P, Streubel B. t(11;14)(q23;q32) involving IGH and DDX6 in nodal marginal zone lymphoma. Genes Chromosomes Cancer. 2013;52:33-43 pubmed publisher
    ..Furthermore, we identified a translocation t(11;14)(q23;q32) involving IGH and DDX6. Chromosomal walking, expression analysis, siRNA-mediated gene knockdown and a yeast two hybrid screen were ..
  65. Onzere C, Bastos A, Okoth E, Lichoti J, Bochere E, Owido M, et al. Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011-2013) reveals rapid B602L central variable region evolution. Virus Genes. 2018;54:111-123 pubmed publisher
    ..Sequencing the p54 gene further discriminates ASFV genotypes that are conserved at the p72 locus...
  66. Ayache J, Bénard M, Ernoult Lange M, Minshall N, Standart N, Kress M, et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell. 2015;26:2579-95 pubmed publisher
    P-bodies are cytoplasmic ribonucleoprotein granules involved in posttranscriptional regulation. DDX6 is a key component of their assembly in human cells...
  67. Baskin R, Woods N, Mendoza Fandiño G, Forsyth P, Egan K, Monteiro A. Functional analysis of the 11q23.3 glioma susceptibility locus implicates PHLDB1 and DDX6 in glioma susceptibility. Sci Rep. 2015;5:17367 pubmed publisher
    ..enhancer scanning assay, we established a subset of 10 functional SNPs in the promoters of PHLDB1 and DDX6, and in a putative enhancer element...
  68. Efthymiou G, Dardiotis E, Liaskos C, Marou E, Tsimourtou V, Rigopoulou E, et al. Immune responses against Helicobacter pylori-specific antigens differentiate relapsing remitting from secondary progressive multiple sclerosis. Sci Rep. 2017;7:7929 pubmed publisher
    ..4%, 48.5%, respectively). Abs against 5 of the 14 antigens were less frequent in RRMS than HCs, including p41, p54-flagellin, p29-UreA, p67-FSH, and p120-CagA. Anti-VacA abs were more frequent in SPMS than in HCs (42.1 vs 12...
  69. Akao Y, Seto M, Yamamoto K, Iida S, Nakazawa S, Inazawa J, et al. The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL-1 gene with t(4;11) and t(11;19) translocations. Cancer Res. 1992;52:6083-7 pubmed
    We previously demonstrated that the 11q23 breakpoint region, designated the RCK locus, of the RC-K8 B-lymphoma cell line with t(11;14)(q23;q32) is centromeric to PBGD, while breakpoints of infantile leukemia cell lines with t(11;19)(q23;..
  70. Bras J, Silva A, Calin G, Barbosa M, Santos S, Almeida M. miR-195 inhibits macrophages pro-inflammatory profile and impacts the crosstalk with smooth muscle cells. PLoS ONE. 2017;12:e0188530 pubmed publisher
    ..In addition, phosphorylated forms of p54 JNK, p46 JNK and p38 MAPK were decreased by miR-195 in macrophages following M1 stimulation...
  71. Lu D, Yunis J. Cloning, expression and localization of an RNA helicase gene from a human lymphoid cell line with chromosomal breakpoint 11q23.3. Nucleic Acids Res. 1992;20:1967-72 pubmed
    A gene encoding a putative human RNA helicase, p54, has been cloned and mapped to the band q23.3 of chromosome 11...
  72. Loedige I, Gaidatzis D, Sack R, Meister G, Filipowicz W. The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function. Nucleic Acids Res. 2013;41:518-32 pubmed publisher
  73. Tang X, Zhang Z, Hu D, Xu A, Zhou H, Sun L, et al. Association analyses identify three susceptibility Loci for vitiligo in the Chinese Han population. J Invest Dermatol. 2013;133:403-10 pubmed publisher
    ..They also highlight similarities and differences in the genetic basis of vitiligo in Chinese and Caucasian populations. ..
  74. Cho Y, Kang W, Kim S, Sa J, Kim N, Paddison P, et al. Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma. Int J Oncol. 2016;48:1053-62 pubmed publisher
    ..We found DDX6 as the candidate gene for treatment resistance after screening and establishing DDX6 knock down cells for ..
  75. Ozgur S, Stoecklin G. Role of Rck-Pat1b binding in assembly of processing-bodies. RNA Biol. 2013;10:528-39 pubmed publisher
    The DEAD box RNA helicase Rck and the scaffold protein Pat1b participate in controlling gene expression at the post-transcriptional level by suppressing mRNA translation and promoting mRNA decapping...
  76. Lind P, Macgregor S, Vink J, Pergadia M, Hansell N, de Moor M, et al. A genomewide association study of nicotine and alcohol dependence in Australian and Dutch populations. Twin Res Hum Genet. 2010;13:10-29 pubmed publisher
    ..90 x 10(-9)), rs1784300 near DDX6 on chromosome 11 (p = 2.60 x 10(-9)) and rs12882384 in KIAA1409 on chromosome 14 (p = 4.86 x 10(-8)))...
  77. Giraldez T, Rothberg B. Understanding the conformational motions of RCK gating rings. J Gen Physiol. 2017;149:431-441 pubmed publisher
    Regulator of conduction of K+ (RCK) domains are ubiquitous regulators of channel and transporter activity in prokaryotes and eukaryotes...
  78. Wiedemann A, Mijouin L, Ayoub M, Barilleau E, Canepa S, Teixeira Gomes A, et al. Identification of the epidermal growth factor receptor as the receptor for Salmonella Rck-dependent invasion. FASEB J. 2016;30:4180-4191 pubmed
    The Salmonella Rck outer membrane protein binds to the cell surface, which leads to bacterial internalization via a Zipper mechanism...
  79. Caster S, Castillo K, Sachs M, Bell Pedersen D. Circadian clock regulation of mRNA translation through eukaryotic elongation factor eEF-2. Proc Natl Acad Sci U S A. 2016;113:9605-10 pubmed publisher
    ..phosphorylation of a eukaryotic elongation factor-2 (eEF-2) kinase, radiation sensitivity complementing kinase-2 (RCK-2), and that RCK-2 is necessary for high-level phosphorylation of eEF-2, a key regulator of translation elongation...
  80. Kamenska A, Simpson C, Vindry C, Broomhead H, Bénard M, Ernoult Lange M, et al. The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic Acids Res. 2016;44:6318-34 pubmed publisher
    ..and delineating the interacting sites of its major partners by mass spectrometry and western blotting, including DDX6, UNR, unrip, PAT1B, LSM14A and CNOT4...
  81. Sanna G, Dei Giudici S, Bacciu D, Angioi P, Giammarioli M, De Mia G, et al. Improved Strategy for Molecular Characterization of African Swine Fever Viruses from Sardinia, Based on Analysis of p30, CD2V and I73R/I329L Variable Regions. Transbound Emerg Dis. 2017;64:1280-1286 pubmed publisher
    ..Previous sequencing data of genes that codify for viral protein p54, p72 and the central variable region (CVR) within the B602L gene revealed that Sardinian isolates show a very low ..
  82. Bladt F, Birchmeier C. Characterization and expression analysis of the murine rck gene: a protein kinase with a potential function in sensory cells. Differentiation. 1993;53:115-22 pubmed
    We have characterized a murine protein kinase gene, rck, which was identified by crosshybridization with sequences from the v-ros tyrosine kinase gene under conditions of reduced stringency...
  83. Lumb J, Li Q, Popov L, Ding S, Keith M, Merrill B, et al. DDX6 Represses Aberrant Activation of Interferon-Stimulated Genes. Cell Rep. 2017;20:819-831 pubmed publisher
    ..Through a genome-wide genetic screen, we identified DEAD-box helicase 6 (DDX6) as a suppressor of ISGs. Genetic ablation of DDX6 induced global upregulation of ISGs and other immune genes...