DCP2

Summary

Gene Symbol: DCP2
Description: decapping mRNA 2
Alias: NUDT20, m7GpppN-mRNA hydrolase, DCP2 decapping enzyme homolog, hDpc, mRNA-decapping enzyme 2, nudix (nucleoside diphosphate linked moiety X)-type motif 20
Species: human
Products:     DCP2

Top Publications

  1. van Dijk E, Cougot N, Meyer S, Babajko S, Wahle E, Seraphin B. Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures. EMBO J. 2002;21:6915-24 pubmed
    We have cloned cDNAs for the human homologues of the yeast Dcp1 and Dcp2 factors involved in the major (5'-3') and NMD mRNA decay pathways...
  2. Li Y, Song M, Kiledjian M. Transcript-specific decapping and regulated stability by the human Dcp2 decapping protein. Mol Cell Biol. 2008;28:939-48 pubmed
    mRNA decapping is a critical step in the control of mRNA stability and gene expression and is carried out by the Dcp2 decapping enzyme. Dcp2 is an RNA binding protein that must bind RNA in order to recognize the cap for hydrolysis...
  3. Lai T, Cho H, Liu Z, Bowler M, Piao S, Parker R, et al. Structural basis of the PNRC2-mediated link between mrna surveillance and decapping. Structure. 2012;20:2025-37 pubmed publisher
    ..PNRC2 acts in synergy with Dcp1a to stimulate the decapping activity of Dcp2 by bridging the interaction between Dcp1a and Dcp2, suggesting that PNRC2 is a decapping coactivator in addition to ..
  4. Wichroski M, Robb G, Rana T. Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. PLoS Pathog. 2006;2:e41 pubmed
    ..suppression (RCK/p54), RNA interference-mediated post-transcriptional gene silencing (AGO2), and decapping of mRNA (DCP2)...
  5. Ingelfinger D, Arndt Jovin D, Luhrmann R, Achsel T. The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci. RNA. 2002;8:1489-501 pubmed
    ..Therefore, the foci contain a partially or fully assembled machinery for the degradation of mRNA. ..
  6. Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol. 2004;165:31-40 pubmed
    ..The occurrence of 5'-3' mRNA decay in specific subcellular locations in human cells suggests that the cytoplasm of eukaryotic cells may be more organized than previously anticipated. ..
  7. Fenger Grøn M, Fillman C, Norrild B, Lykke Andersen J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Mol Cell. 2005;20:905-15 pubmed
    ..These observations suggest that multiple proteins involved in human decapping are important subunits of PBs and are activated on ARE-mRNAs by the protein TTP. ..
  8. Wang Z, Jiao X, Carr Schmid A, Kiledjian M. The hDcp2 protein is a mammalian mRNA decapping enzyme. Proc Natl Acad Sci U S A. 2002;99:12663-8 pubmed
    ..We identified the human Dcp2 protein (hDcp2) as an enzyme containing intrinsic decapping activity...
  9. Lykke Andersen J. Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay. Mol Cell Biol. 2002;22:8114-21 pubmed
    ..These data suggest that a human decapping complex may be recruited to mRNAs containing premature termination codons by the hUpf proteins...

More Information

Publications87

  1. Liu J, Valencia Sanchez M, Hannon G, Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat Cell Biol. 2005;7:719-23 pubmed
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  2. Tritschler F, Braun J, Motz C, Igreja C, Haas G, Truffault V, et al. DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa. Proc Natl Acad Sci U S A. 2009;106:21591-6 pubmed publisher
    DCP1 stimulates the decapping enzyme DCP2, which removes the mRNA 5' cap structure committing mRNAs to degradation. In multicellular eukaryotes, DCP1-DCP2 interaction is stabilized by additional proteins, including EDC4...
  3. Piccirillo C, Khanna R, Kiledjian M. Functional characterization of the mammalian mRNA decapping enzyme hDcp2. RNA. 2003;9:1138-47 pubmed
  4. Lejeune F, Li X, Maquat L. Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities. Mol Cell. 2003;12:675-87 pubmed
    ..To date, the polarity and enzymology of NMD in mammalian cells is unknown. We show here that downregulating the Dcp2 decapping protein or the PM/Scl100 component of the exosome (1) significantly increases the abundance of steady-..
  5. Bloch D, Nobre R, Bernstein G, Yang W. Identification and characterization of protein interactions in the mammalian mRNA processing body using a novel two-hybrid assay. Exp Cell Res. 2011;317:2183-99 pubmed publisher
    ..The assay was used to investigate interactions between P-body components Ge-1, DCP2, DCP1, EDC3, RAP55, and RCK...
  6. Aglietti R, Floor S, McClendon C, Jacobson M, Gross J. Active site conformational dynamics are coupled to catalysis in the mRNA decapping enzyme Dcp2. Structure. 2013;21:1571-80 pubmed publisher
    Removal of the 5' cap structure by Dcp2 is a major step in several 5'-3' mRNA decay pathways...
  7. Mikuda N, Kolesnichenko M, Beaudette P, Popp O, Uyar B, Sun W, et al. The IκB kinase complex is a regulator of mRNA stability. EMBO J. 2018;37: pubmed publisher
    ..manner, leading to co-recruitment of P body components, mRNA decapping proteins 1a and 2 (DCP1a and DCP2) and to an increase in P body numbers...
  8. Nishimura T, Fakim H, Brandmann T, Youn J, Gingras A, Jinek M, et al. Human MARF1 is an endoribonuclease that interacts with the DCP1:2 decapping complex and degrades target mRNAs. Nucleic Acids Res. 2018;46:12008-12021 pubmed publisher
    ..MARF1 physically interacts with the DCP1:DCP2 mRNA decapping complex but not with deadenylation machineries. Importantly, we provide a 1...
  9. He F, Li C, Roy B, Jacobson A. Yeast Edc3 targets RPS28B mRNA for decapping by binding to a 3' untranslated region decay-inducing regulatory element. Mol Cell Biol. 2014;34:1438-51 pubmed publisher
    ..In yeast, general mRNA decapping requires the Dcp1/Dcp2 decapping enzyme and a set of decapping activators, including Pat1, Dhh1, Edc3, and the Lsm1-7 complex...
  10. Bednarek S, Madan V, Sikorski P, Bartenschlager R, Kowalska J, Jemielity J. mRNAs biotinylated within the 5' cap and protected against decapping: new tools to capture RNA-protein complexes. Philos Trans R Soc Lond B Biol Sci. 2018;373: pubmed publisher
    ..quantification of cap-dependent translation efficiency, capping efficiency and the susceptibility to decapping by Dcp2. The utility of such cap-biotinylated RNAs as molecular tool was demonstrated by ultraviolet-cross-linking and ..
  11. Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido B, et al. Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. EMBO J. 2018;37: pubmed publisher
    ..We found that PNRC1 interacts with the cytoplasmic DCP1α/DCP2 decapping machinery and hauls it inside the nucleolus...
  12. Charenton C, Graille M. mRNA decapping: finding the right structures. Philos Trans R Soc Lond B Biol Sci. 2018;373: pubmed publisher
    ..The decapping reaction is catalysed by a multi-protein complex formed by the Dcp2 catalytic subunit and its Dcp1 cofactor, a holoenzyme that is poorly active on its own and needs several accessory ..
  13. Ma X, Nicole M, Meteignier L, Hong N, Wang G, Moffett P. Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants. J Exp Bot. 2015;66:919-32 pubmed publisher
    ..Consistent with this, we found that viral recovery induced increased PB formation and that a decapping mutant (DCP2) showed increased VIGS and virus RNA accumulation, indicating an important role for PBs in eliminating viral RNA.
  14. Zhang M, Tang Q, Li R, Song M. MicroRNA-141-3p/200a-3p target and may be involved in post-transcriptional repression of RNA decapping enzyme Dcp2 during renal development. Biosci Biotechnol Biochem. 2018;82:1724-1732 pubmed publisher
    The RNA decapping enzyme Dcp2 is a crucial enzyme involved in the process of RNA turnover, which can post-transcriptionally regulate gene expression. Dcp2 has been found to be highly expressed in embryonic, but not adult, kidneys...
  15. Ghiasi S, Krogh N, Tyrberg B, Mandrup Poulsen T. The No-Go and Nonsense-Mediated RNA Decay Pathways Are Regulated by Inflammatory Cytokines in Insulin-Producing Cells and Human Islets and Determine ?-Cell Insulin Biosynthesis and Survival. Diabetes. 2018;67:2019-2037 pubmed publisher
    ..Pelo, Dcp2, Dis3L2, Upf2, and Smg1/5/6/7 were upregulated by inflammatory cytokines in INS-1 cells under conditions where ..
  16. Floor S, Jones B, Hernandez G, Gross J. A split active site couples cap recognition by Dcp2 to activation. Nat Struct Mol Biol. 2010;17:1096-101 pubmed publisher
    Decapping by Dcp2 is an essential step in 5'-to-3' mRNA decay. In yeast, decapping requires an open-to-closed transition in Dcp2, though the link between closure and catalysis remains elusive...
  17. Raju K, Natarajan S, Kumar N, Kumar D, N M R. Role of cytoplasmic deadenylation and mRNA decay factors in yeast apoptosis. FEMS Yeast Res. 2015;15: pubmed publisher
    Strains of Saccharomyces cerevisiae lacking factors involved in 5' to 3' mRNA decay pathway (DCP1, DCP2, DHH1, PAT1, LSM1 and LSM4) exhibit caspase-dependent apoptosis and accelerated chronological aging...
  18. Motomura K, Le Q, Hamada T, Kutsuna N, Mano S, Nishimura M, et al. Diffuse decapping enzyme DCP2 accumulates in DCP1 foci under heat stress in Arabidopsis thaliana. Plant Cell Physiol. 2015;56:107-15 pubmed publisher
    The decapping enzymes DCP1 and DCP2 are components of a decapping complex that degrades mRNAs. DCP2 is the catalytic core and DCP1 is an auxiliary subunit...
  19. Aizer A, Brody Y, Ler L, Sonenberg N, Singer R, Shav Tal Y. The dynamics of mammalian P body transport, assembly, and disassembly in vivo. Mol Biol Cell. 2008;19:4154-66 pubmed publisher
    ..FRAP analysis showed that the decapping enzyme Dcp2 is a nondynamic PB core protein, whereas Dcp1 proteins continuously exchanged with the cytoplasm...
  20. Higa M, Kita A, Hagihara K, Kitai Y, Doi A, Nagasoko R, et al. Spatial control of calcineurin in response to heat shock in fission yeast. Genes Cells. 2015;20:95-107 pubmed publisher
    ..The calcineurin dots colocalized with Dcp2 or Pabp, the constituent of P-bodies or stress granules, respectively, thus suggesting that calcineurin is a ..
  21. Ahmed I, Buchert R, Zhou M, Jiao X, Mittal K, Sheikh T, et al. Mutations in DCPS and EDC3 in autosomal recessive intellectual disability indicate a crucial role for mRNA decapping in neurodevelopment. Hum Mol Genet. 2015;24:3172-80 pubmed publisher
    ..161T>C; p.Phe54Ser) in EDC3 in two affected children. EDC3 stimulates DCP2, which decaps mRNAs at the beginning of the 5' to 3' degradation pathway...
  22. Taverniti V, Séraphin B. Elimination of cap structures generated by mRNA decay involves the new scavenger mRNA decapping enzyme Aph1/FHIT together with DcpS. Nucleic Acids Res. 2015;43:482-92 pubmed publisher
    ..In the 5'-3' pathway, the decapping enzyme Dcp2 generates m7GDP. We investigated the fate of m7GDP and m7GpppN produced by RNA decay in extracts and cells...
  23. Grudzien Nogalska E, Jiao X, Song M, Hart R, Kiledjian M. Nudt3 is an mRNA decapping enzyme that modulates cell migration. RNA. 2016;22:773-81 pubmed publisher
    ..The Nudix hydrolase, Dcp2, was identified as a first decapping enzyme and subsequently shown to preferentially modulate stability of only a ..
  24. Chiang P, Shen Y, Su Y, Kao C, Lin N, Hsu P, et al. Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes. PLoS ONE. 2013;8:e61697 pubmed publisher
    ..vasodilator-stimulated protein homology 1) domain in the N terminus of Dcp1a showed a stronger interaction with Dcp2. Once ERK signaling was activated, the interaction between Dcp1a and Ddx6, Edc3, or Edc4 was not affected by Dcp1a ..
  25. Mugridge J, Tibble R, Ziemniak M, Jemielity J, Gross J. Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis. Nat Commun. 2018;9:1152 pubmed publisher
    The conserved decapping enzyme Dcp2 recognizes and removes the 5' eukaryotic cap from mRNA transcripts in a critical step of many cellular RNA decay pathways...
  26. Valkov E, Muthukumar S, Chang C, Jonas S, Weichenrieder O, Izaurralde E. Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Nat Struct Mol Biol. 2016;23:574-9 pubmed publisher
    The removal of the mRNA 5' cap (decapping) by Dcp2 shuts down translation and commits mRNA to full degradation. Dcp2 activity is enhanced by activator proteins such as Dcp1 and Edc1...
  27. Ma J, Fukuda Y, Schultz R. Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation. Biol Reprod. 2015;93:48 pubmed publisher
    ..demonstrated that phosphorylation of MSY2, an RNA-binding protein, and mobilization of mRNAs encoding the DCP1A-DCP2 decapping complex contribute to maternal mRNA destruction during meiotic maturation...
  28. Grzela R, Nasilowska K, Lukaszewicz M, Tyras M, Stepinski J, Jankowska Anyszka M, et al. Hydrolytic activity of human Nudt16 enzyme towards dinucleotide cap analogs and short capped oligonucleotides. RNA. 2018;: pubmed publisher
    ..cytoplasmic localization of mammalian Nudt16 with cap hydrolysis activity initiating RNA turnover, similar to Dcp2. The present study focuses on hNudt16 and its hydrolytic activity towards dinucleotide cap analogs and short capped ..
  29. Huch S, Gommlich J, Muppavarapu M, Beckham C, Nissan T. Membrane-association of mRNA decapping factors is independent of stress in budding yeast. Sci Rep. 2016;6:25477 pubmed publisher
    ..In this study, we examined the mRNA decapping proteins, Dcp1, Dcp2, and Dhh1, using subcellular fractionation...
  30. Grudzien Nogalska E, Kiledjian M. New insights into decapping enzymes and selective mRNA decay. Wiley Interdiscip Rev RNA. 2017;8: pubmed publisher
    ..The Nudix hydrolase, Dcp2, identified as a first decapping enzyme, cleaves capped mRNA and initiates 5'-3' degradation...
  31. Gunawardana D. PDZ Binding Domains, Structural Disorder and Phosphorylation: A Menage-a-trois Tailing Dcp2 mRNA Decapping Enzymes. Protein Pept Lett. 2016;23:612-8 pubmed
    ..We identified and characterized a Dcp2 type mRNA decapping enzyme from Arabidopsis thaliana, a protein containing a putative PDZ-binding domain using ..
  32. Kiledjian M, Zhou M, Jiao X. Normal and Aberrantly Capped mRNA Decapping. Enzymes. 2012;31:165-80 pubmed publisher
    ..At least two decapping enzymes, Dcp2 and Nudt16, and an array of decapping regulatory proteins remove the m(7)G cap from an mRNA exposing the 5'-end to ..
  33. Charenton C, Taverniti V, Gaudon Plesse C, Back R, Seraphin B, Graille M. Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator. Nat Struct Mol Biol. 2016;23:982-986 pubmed publisher
    ..Decapping is accomplished by the recruitment of a protein complex formed by the Dcp2 catalytic subunit and its Dcp1 cofactor...
  34. Kramer S. The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes. PLoS Pathog. 2017;13:e1006456 pubmed publisher
    ..After deadenylation, mRNAs are decapped by the nudix hydrolase DCP2 of the decapping complex and finally degraded by the 5'-3' exoribonuclease...
  35. Ye J, Yang J, Sun Y, Zhao P, Gao S, Jung C, et al. Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis. PLoS Pathog. 2015;11:e1005196 pubmed publisher
    ..We found that AS2 promotes DCP2 decapping activity, accelerates mRNA turnover rate, inhibits siRNA accumulation and functions as an endogenous ..
  36. Chuang T, Lee K, Lou Y, Lu C, Tarn W. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem. 2016;291:8565-74 pubmed publisher
    ..Moreover, Y14 binds the cap structure of mRNAs and inhibits the activity of the decapping enzyme Dcp2. In this report, we show that an evolutionarily conserved tryptophan residue (Trp-73) of Y14 is critical for its ..
  37. Huch S, Müller M, Muppavarapu M, Gommlich J, Balagopal V, Nissan T. The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae. Biol Open. 2016;5:1388-1399 pubmed publisher
    ..of specific mRNA decay factors as well as nuclear accumulation of the catalytic subunit of the decapping enzyme Dcp2. Hence, we suggest that the effects on mRNA stability in the edc3? lsm4?C mutant may originate from mRNA decay ..
  38. Reed J, Westergreen N, Barajas B, Ressler D, Phuong D, Swain J, et al. Formation of RNA Granule-Derived Capsid Assembly Intermediates Appears To Be Conserved between Human Immunodeficiency Virus Type 1 and the Nonprimate Lentivirus Feline Immunodeficiency Virus. J Virol. 2018;92: pubmed publisher
    ..Additionally, we showed that FIV Gag associates with another RNA granule protein, DCP2. Finally, we validated the FIV Gag-ABCE1 and FIV Gag-DCP2 interactions with proximity ligation assays demonstrating ..
  39. He F, Jacobson A. Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain. RNA. 2015;21:1633-47 pubmed publisher
    ..cerevisiae, a single decapping enzyme composed of a regulatory subunit (Dcp1) and a catalytic subunit (Dcp2) targets thousands of distinct substrate mRNAs...
  40. Ziemniak M, Mugridge J, Kowalska J, Rhoads R, Gross J, Jemielity J. Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex. RNA. 2016;22:518-29 pubmed publisher
    Dcp1/2 is the major eukaryotic RNA decapping complex, comprised of the enzyme Dcp2 and activator Dcp1, which removes the 5' m(7)G cap from mRNA, committing the transcript to degradation...
  41. Mauer J, Luo X, Blanjoie A, Jiao X, Grozhik A, Patil D, et al. Reversible methylation of m6Am in the 5' cap controls mRNA stability. Nature. 2017;541:371-375 pubmed publisher
    ..stability of m6Am-initiated transcripts is due to resistance to the mRNA-decapping enzyme DCP2. Moreover, we find that m6Am is selectively demethylated by fat mass and obesity-associated ..
  42. Wurm J, Holdermann I, Overbeck J, Mayer P, Sprangers R. Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme. Proc Natl Acad Sci U S A. 2017;114:6034-6039 pubmed publisher
    ..Here, we study the bilobed decapping enzyme Dcp2 that removes the 5' cap structure from eukaryotic mRNA and thereby efficiently terminates gene expression...
  43. Mugridge J, Ziemniak M, Jemielity J, Gross J. Structural basis of mRNA-cap recognition by Dcp1-Dcp2. Nat Struct Mol Biol. 2016;23:987-994 pubmed publisher
    Removal of the 5' cap on mRNA by the decapping enzyme Dcp2 is a critical step in 5'-to-3' mRNA decay...
  44. Wang C, Wang Y, Hsiao W, Wang S. Involvement of fission yeast Pdc2 in RNA degradation and P-body function. RNA. 2017;23:493-503 pubmed publisher
    ..We found that Pdc2 interacts with Dcp2 and is required for decapping in vivo...
  45. Qi M, Song J, Zhang Z, Huang S, Jing Q. P38 activation induces the dissociation of tristetraprolin from Argonaute 2 to increase ARE-mRNA stabilization. Mol Biol Cell. 2018;29:988-1002 pubmed publisher
    ..P38 plays a crucial role in stabilizing ARE-mRNA. Here we reveal that P38 activation represses the interaction between TTP and Ago2, thus restraining TTP from being targeted into processing bodies and stabilizing ARE-mRNA. ..
  46. Wurm J, Overbeck J, Sprangers R. The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface. RNA. 2016;22:1360-72 pubmed publisher
    ..mRNA cap structure (decapping) is a central step in the 5'-3' mRNA degradation pathway and is performed by the Dcp1:Dcp2 decapping complex...
  47. Shapouri F, Saeidi S, de Iongh R, Casagranda F, Western P, McLaughlin E, et al. Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2. Cell Tissue Res. 2016;364:443-51 pubmed publisher
    ..The cytoplasmic presence of Tob1 protein in round spermatids and oocytes and the association of Tob1 protein with Dcp2 in both cell types suggest that Tob1 protein plays a role in post-transcriptional mechanisms.
  48. Shukla S, Schmidt J, Goldfarb K, Cech T, Parker R. Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects. Nat Struct Mol Biol. 2016;23:286-92 pubmed publisher
    ..which promotes 3'-to-5' degradation by EXOSC10, as well as decapping and 5'-to-3' decay by the cytoplasmic DCP2 and XRN1 enzymes. PARN increased hTR levels by deadenylating hTR, thereby limiting its degradation by EXOSC10...
  49. Valkov E, Jonas S, Weichenrieder O. Mille viae in eukaryotic mRNA decapping. Curr Opin Struct Biol. 2017;47:40-51 pubmed publisher
    ..Since the removal of the mRNA 5'-cap by the decapping enzyme DCP2 is generally an irreversible step towards decay, it requires regulation...
  50. Lykke Andersen J, Wagner E. Recruitment and activation of mRNA decay enzymes by two ARE-mediated decay activation domains in the proteins TTP and BRF-1. Genes Dev. 2005;19:351-61 pubmed
    ..This describes a potentially regulated step in activation of mRNA decay. ..
  51. Yamochi T, Ohnuma K, Hosono O, Tanaka H, Kanai Y, Morimoto C. SSA/Ro52 autoantigen interacts with Dcp2 to enhance its decapping activity. Biochem Biophys Res Commun. 2008;370:195-9 pubmed publisher
    ..Our data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation. ..
  52. Chang C, Bercovich N, Loh B, Jonas S, Izaurralde E. The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1. Nucleic Acids Res. 2014;42:5217-33 pubmed publisher
    The removal of the 5'-cap structure by the decapping enzyme DCP2 and its coactivator DCP1 shuts down translation and exposes the mRNA to 5'-to-3' exonucleolytic degradation by XRN1...
  53. Charenton C, Gaudon Plesse C, Fourati Z, Taverniti V, Back R, Kolesnikova O, et al. A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5'-3' mRNA exonuclease in yeast. Proc Natl Acad Sci U S A. 2017;114:E9493-E9501 pubmed publisher
    ..with several short motifs, named helical leucine-rich motifs (HLMs), spread in the long C-terminal region of yeast Dcp2 decapping enzyme. Structures of Pat1-HLM complexes reveal the basis for HLM recognition by Pat1...
  54. Balagopal V, Beemon K. Rous Sarcoma Virus RNA Stability Element Inhibits Deadenylation of mRNAs with Long 3'UTRs. Viruses. 2017;9: pubmed publisher
    ..We show here that the presence of RSE inhibits deadenylation severely. In addition, the RSE also impairs decapping (DCP2) and 5'-3' exonucleolytic (XRN1) function in knockdown experiments in human cells.
  55. James V, Zhang Y, Foxler D, de Moor C, Kong Y, Webb T, et al. LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proc Natl Acad Sci U S A. 2010;107:12499-504 pubmed publisher
    ..Furthermore, we reveal that LIMD1, Ajuba, and WTIP bind to Ago1/2, RCK, Dcp2, and eIF4E in vivo, that they are required for miRNA-mediated, but not siRNA-mediated gene silencing and that all ..
  56. Paquette D, Mugridge J, Weinberg D, Gross J. Application of a Schizosaccharomyces pombe Edc1-fused Dcp1-Dcp2 decapping enzyme for transcription start site mapping. RNA. 2017;: pubmed publisher
    ..This engineered enzyme is a better replacement for TAP treatment than the current marketed use of RppH and can be produced cost-effectively in a general lab setting, unlike Cap-Clip. ..
  57. Liu S, Jiao X, Liu H, Gu M, Lima C, Kiledjian M. Functional analysis of mRNA scavenger decapping enzymes. RNA. 2004;10:1412-22 pubmed
    ..These data reveal that both decapping enzymes are contained in the nuclear compartment, indicating that they may fulfill a greater function in the nucleus than previously appreciated. ..
  58. Song M, Li Y, Kiledjian M. Multiple mRNA decapping enzymes in mammalian cells. Mol Cell. 2010;40:423-32 pubmed publisher
    ..b>Dcp2 is currently believed to be the only cytoplasmic decapping enzyme responsible for decapping of all mRNAs...
  59. Ozgur S, Chekulaeva M, Stoecklin G. Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies. Mol Cell Biol. 2010;30:4308-23 pubmed publisher
    ..Pat1b as a protein that is tightly associated with the Ccr4-Caf1-Not deadenylation complex as well as with the Dcp1-Dcp2 decapping complex. In addition, the RNA helicase Rck and Lsm1 proteins interact with human Pat1b...
  60. Mishima Y, Tomari Y. Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish. Genes Cells. 2017;22:670-678 pubmed publisher
    ..Here, we analyzed a contribution of the decapping enzyme Dcp2 to maternal mRNA clearance in zebrafish by over-expressing catalytically inactive Dcp2 and performing RNA-seq ..
  61. Fromm S, Kamenz J, Nöldeke E, Neu A, Zocher G, Sprangers R. In vitro reconstitution of a cellular phase-transition process that involves the mRNA decapping machinery. Angew Chem Int Ed Engl. 2014;53:7354-9 pubmed publisher
    ..We show that the Schizosaccharomyces pombe Dcp2 mRNA decapping enzyme, its prime activator Dcp1, and the scaffolding proteins Edc3 and Pdc1 are sufficient to ..
  62. Braun J, Tritschler F, Haas G, Igreja C, Truffault V, Weichenrieder O, et al. The C-terminal alpha-alpha superhelix of Pat is required for mRNA decapping in metazoa. EMBO J. 2010;29:2368-80 pubmed publisher
    ..We show that Pat-C is essential for the interaction with mRNA decapping factors (i.e. DCP2, EDC4 and LSm1-7), whereas the P-rich region and Mid domain have distinct functions in modulating these ..
  63. Mullen T, Marzluff W. Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'. Genes Dev. 2008;22:50-65 pubmed publisher
    ..RNAi experiments demonstrate that both the exosome and 5'-to-3' decay pathway components are required for degradation, and individual histone mRNAs are then degraded simultaneously 5' to 3' and 3' to 5'. ..
  64. Ueno K, Kumagai T, Kijima T, Kishimoto T, Hosoe S. Cloning and tissue expression of cDNAs from chromosome 5q21-22 which is frequently deleted in advanced lung cancer. Hum Genet. 1998;102:63-8 pubmed
    ..This large contig and expression map should prove crucial in the identification of susceptibility gene(s) related to the progression of lung cancer. ..
  65. Reed J, Molter B, Geary C, McNevin J, McElrath J, Giri S, et al. HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. J Cell Biol. 2012;198:439-56 pubmed publisher
    ..Thus, we propose that assembling HIV-1 co-opts a preexisting host complex containing cellular facilitators such as DDX6, which the virus uses to catalyze capsid assembly...
  66. Chu C, Rana T. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol. 2006;4:e210 pubmed
    ..These studies also suggest that translation suppression by miRISC does not require P-body structures, and location of miRISC to P-bodies is the consequence of translation repression...
  67. Grzymski E. Visualizing an mRNA destruction line. Nat Struct Biol. 2003;10:416 pubmed
  68. Adachi T, Nagahama K, Izumi S. The C. elegans mRNA decapping enzyme shapes morphology of cilia. Biochem Biophys Res Commun. 2017;493:382-387 pubmed publisher
    ..Here we show that DCAP-1 and DCAP-2, which are the homologues of mammalian DCP1 and DCP2 mRNA decapping enzymes, respectively, are involved in formation of dual rod-type and wing-like shaped cilia in C...
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    ..Although the mechanism underlying this inhibition is still unclear, it appears to target BRF1-dependent AMD at a level downstream from RNA binding and the recruitment of mRNA decay enzymes. ..
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    ..In contrast, the P-body markers DCP2, LSM1, eIF4e, DDX6, and AGO1 were in LMM complexes, whereas AGO2, an effector protein of the RNA-induced silencing ..
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    ..We also demonstrate that Y14 interacts directly with the decapping factor Dcp2 and the 5' cap structure of mRNAs via different but overlapping domains and that Y14 inhibits the mRNA-decapping ..
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    ..C-terminal lysines of DCP1a suppresses decapping activity and impairs the interaction with the mRNA decay factors DCP2, EDC4, and XRN1, but not EDC3, thus remodeling P-body architecture...
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    ..mechanism of autophagy regulation by RNA helicase RCK family members in association with the decapping enzyme Dcp2. Under nutrient-replete conditions, Dcp2 undergoes TOR-dependent phosphorylation and associates with RCK members to ..
  74. Zhang Y, Li W, Ma Y, Wang D, Zhao X, Zeng C, et al. Improved development by melatonin treatment after vitrification of mouse metaphase II oocytes. Cryobiology. 2016;73:335-342 pubmed publisher
    ..and PA, the ROS levels increased significantly and maternal-to-zygotic transition (MZT) related genes (Dcp1a, Dcp2, Hspa1a, Eif1ax, Pou5f1, Sox2) expression were disorganized...
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    ..In addition, compounds 1-3 showed inhibitory activity in the cysteine aspartyl-specific protease-3 (caspase-3) enzymatic assay, with IC?? values of 20.6, 10.9, and 7.9?µM, respectively. ..
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    ..Our results indicate that ZAP inhibits HIV-1 by recruiting both the 5' and 3' mRNA degradation machinery to specifically promote the degradation of multiply spliced HIV-1 mRNAs. ..
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    ..This recruitment requires Hrr25 kinase activity and the Dcp2 decapping enzyme, a core constituent of these RNP granules...
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    ..A major complex responsible for decapping consists of the decapping enzyme Dcp2 in association with decapping enhancers...