CUL5

Summary

Gene Symbol: CUL5
Description: cullin 5
Alias: CUL-5, VACM-1, VACM1, cullin-5, Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1), Vasopressin-activated calcium-mobilizing receptor-1, vasopressin-activated calcium-mobilizing receptor 1
Species: human
Products:     CUL5

Top Publications

  1. Burnatowska Hledin M, Zhao P, Capps B, Poel A, Parmelee K, Mungall C, et al. VACM-1, a cullin gene family member, regulates cellular signaling. Am J Physiol Cell Physiol. 2000;279:C266-73 pubmed
    ..Our data demonstrate that VACM-1, the first mammalian cullin protein to be characterized, is involved in the regulation of signaling...
  2. Giri K, Scott R, Maynard E. Molecular structure and biochemical properties of the HCCH-Zn2+ site in HIV-1 Vif. Biochemistry. 2009;48:7969-78 pubmed publisher
    ..Vif recruits a Cullin 5 (Cul5)-based ubiquitin ligase that targets a host cytidine deaminase, apolipoprotein B mRNA editing enzyme ..
  3. Xiao Z, Ehrlich E, Yu Y, Luo K, Wang T, Tian C, et al. Assembly of HIV-1 Vif-Cul5 E3 ubiquitin ligase through a novel zinc-binding domain-stabilized hydrophobic interface in Vif. Virology. 2006;349:290-9 pubmed
    APOBEC3G (A3G) and related cytidine deaminases are potent inhibitors of retroviruses. HIV-1 Vif hijacks the cellular Cul5-E3 ubiquitin ligase to degrade APOBEC3 proteins and render them ineffective against these viruses...
  4. Dehart J, Bosque A, Harris R, Planelles V. Human immunodeficiency virus type 1 Vif induces cell cycle delay via recruitment of the same E3 ubiquitin ligase complex that targets APOBEC3 proteins for degradation. J Virol. 2008;82:9265-72 pubmed publisher
    Human immunodeficiency virus type 1 (HIV-1) Vif recruits a Cullin 5 ubiquitin ligase that targets APOBEC3 proteins for degradation. Recently, Vif has also been shown to induce cell cycle disturbance in G(2)...
  5. Debrincat M, Zhang J, Willson T, Silke J, Connolly L, Simpson R, et al. Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation. J Biol Chem. 2007;282:4728-37 pubmed
    ..The targeting of CKB for degradation by Asb-9 was primarily SOCS box-dependent and suggests that Asb-9 acts as a specific ubiquitin ligase regulating levels of this evolutionarily conserved enzyme. ..
  6. Xiao Z, Ehrlich E, Luo K, Xiong Y, Yu X. Zinc chelation inhibits HIV Vif activity and liberates antiviral function of the cytidine deaminase APOBEC3G. FASEB J. 2007;21:217-22 pubmed
    ..Vif acts as a substrate receptor for the Cullin5 (Cul5) E3 ubiquitin ligase, specifically interacting with Cul5 through a novel H-(x5)-C-(x17-18)-C-(x3-5)-H zinc binding ..
  7. Pery E, Rajendran K, Brazier A, Gabuzda D. Regulation of APOBEC3 proteins by a novel YXXL motif in human immunodeficiency virus type 1 Vif and simian immunodeficiency virus SIVagm Vif. J Virol. 2009;83:2374-81 pubmed publisher
    ..and APOBEC3C (A3C) and targets these proteins for polyubiquitination by forming an E3 ubiquitin ligase with cullin 5. Previous studies identified regions of HIV-1 Vif, 40YRHHY44 and 12QVDRMR17, which are important for interaction ..
  8. Kobayashi M, Takaori Kondo A, Miyauchi Y, Iwai K, Uchiyama T. Ubiquitination of APOBEC3G by an HIV-1 Vif-Cullin5-Elongin B-Elongin C complex is essential for Vif function. J Biol Chem. 2005;280:18573-8 pubmed
    ..by forming an SCF-like E3 ubiquitin ligase complex composed of Cullin5, Elongin B, and Elongin C (Vif-BC-Cul5) through a novel SOCS-box motif...
  9. Donahue J, Vetter M, Mukhtar N, D Aquila R. The HIV-1 Vif PPLP motif is necessary for human APOBEC3G binding and degradation. Virology. 2008;377:49-53 pubmed publisher
    ..We demonstrate that the failure of the Vif mutant to bind A3G resulted in A3G incorporation into assembling virions with loss of viral infectivity. ..

More Information

Publications74

  1. Liu J, Furukawa M, Matsumoto T, Xiong Y. NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 2002;10:1511-8 pubmed
    ..We suggest that by restricting SKP1-CUL1 interaction, CAND1 regulated the assembly of productive SCF ubiquitin ligases, allowing a common CUL1-ROC core to be utilized by a large number of SKP1-F box-substrate subcomplexes. ..
  2. Kelsall I, Duda D, Olszewski J, Hofmann K, Knebel A, Langevin F, et al. TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes. EMBO J. 2013;32:2848-60 pubmed publisher
    ..Cumulatively, our work proposes a conserved mechanism of CRL-induced Ariadne RBR ligase activation and further suggests a reciprocal role of this special class of RBRs as regulators of distinct CRLs. ..
  3. Greene W, Debyser Z, Ikeda Y, Freed E, Stephens E, Yonemoto W, et al. Novel targets for HIV therapy. Antiviral Res. 2008;80:251-65 pubmed publisher
  4. Kershaw N, Laktyushin A, Nicola N, Babon J. Reconstruction of an active SOCS3-based E3 ubiquitin ligase complex in vitro: identification of the active components and JAK2 and gp130 as substrates. Growth Factors. 2014;32:1-10 pubmed publisher
    ..SOCS3-induced polyubiquitination was rapid and could proceed through a number of different ubiquitin lysines. SOCS3 catalyzed the ubiquitination of both the IL-6 receptor common chain (gp130) and JAK2. ..
  5. Stanley B, Ehrlich E, Short L, Yu Y, Xiao Z, Yu X, et al. Structural insight into the human immunodeficiency virus Vif SOCS box and its role in human E3 ubiquitin ligase assembly. J Virol. 2008;82:8656-63 pubmed publisher
    ..Furthermore, our homology modeling reveals that the Vif Cullin box and zinc finger motif may be positioned adjacent to the N terminus of Cullin5 for interaction with loop regions in the first cullin repeat of Cullin5. ..
  6. Wolfe L, Stanley B, Liu C, Eliason W, Xiong Y. Dissection of the HIV Vif interaction with human E3 ubiquitin ligase. J Virol. 2010;84:7135-9 pubmed publisher
    The human immunodeficiency virus type 1 (HIV-1) protein Vif recruits the host E3 ubiquitin ligase, composed of cullin 5 (Cul5), Rbx2, Elongin B, and Elongin C (EloBC), to polyubiquitinate the antiviral protein APOBEC3G...
  7. Duda D, Borg L, Scott D, Hunt H, Hammel M, Schulman B. Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation. Cell. 2008;134:995-1006 pubmed publisher
    ..Here we report striking conformational rearrangements in the crystal structure of NEDD8~Cul5(ctd)-Rbx1 and SAXS analysis of NEDD8~Cul1(ctd)-Rbx1 relative to their unmodified counterparts...
  8. Tan M, Gu Q, He H, Pamarthy D, Semenza G, Sun Y. SAG/ROC2/RBX2 is a HIF-1 target gene that promotes HIF-1 alpha ubiquitination and degradation. Oncogene. 2008;27:1404-11 pubmed
    ..Induced SAG then promotes HIF-1alpha ubiquitination and degradation. This feedback loop may serve as a cellular defensive mechanism to reduce potential cytotoxic effects of prolonged HIF-1 activation under hypoxia. ..
  9. Lyapina S, Cope G, Serino G, Tsuge T, Zhou C, Wolf D, et al. Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome. Science. 2001;292:1382-5 pubmed
  10. Bergeron J, Huthoff H, Veselkov D, Beavil R, Simpson P, Matthews S, et al. The SOCS-box of HIV-1 Vif interacts with ElonginBC by induced-folding to recruit its Cul5-containing ubiquitin ligase complex. PLoS Pathog. 2010;6:e1000925 pubmed publisher
    ..Vif) protein recruits an E3 ubiquitin ligase complex, comprising the cellular proteins elongin B and C (EloBC), cullin 5 (Cul5) and RING-box 2 (Rbx2), to the anti-viral proteins APOBEC3G (A3G) and APOBEC3F (A3F) and induces their ..
  11. Zadjali F, Pike A, Vesterlund M, Sun J, Wu C, Li S, et al. Structural basis for c-KIT inhibition by the suppressor of cytokine signaling 6 (SOCS6) ubiquitin ligase. J Biol Chem. 2011;286:480-90 pubmed publisher
    ..Our data support a role of SOCS6 as a feedback inhibitor of SCF-dependent signaling and provides molecular data to account for target specificity within the SOCS family of ubiquitin ligases. ..
  12. Kamura T, Burian D, Yan Q, Schmidt S, Lane W, Querido E, et al. Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase. J Biol Chem. 2001;276:29748-53 pubmed
    ..MUF1, which we demonstrate is capable of assembling with a Cullin/Rbx1 module containing the Cullin family member Cul5 to reconstitute ubiquitin ligase activity...
  13. Dang Y, Wang X, York I, Zheng Y. Identification of a critical T(Q/D/E)x5ADx2(I/L) motif from primate lentivirus Vif proteins that regulate APOBEC3G and APOBEC3F neutralizing activity. J Virol. 2010;84:8561-70 pubmed publisher
    ..as an adaptor that binds to the cytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) and bridges them to a cullin 5 (Cul5) and elongin (Elo) B/C E3 ubiquitin ligase complex for proteasomal degradation...
  14. Kim Y, Kwak M, Ku B, Suh H, Joo K, Lee J, et al. Structural basis of intersubunit recognition in elongin BC-cullin 5-SOCS box ubiquitin-protein ligase complexes. Acta Crystallogr D Biol Crystallogr. 2013;69:1587-97 pubmed publisher
    ..It is presently poorly understood how cullin-2 (Cul2) and cullin-5 (Cul5) associate specifically with their adaptor elongin BC and a SOCS-box-containing substrate receptor...
  15. Mehle A, Goncalves J, Santa Marta M, McPike M, Gabuzda D. Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation. Genes Dev. 2004;18:2861-6 pubmed
    ..We report here that Vif targets APOBEC3G for degradation by forming an SCF-like E3 ubiquitin ligase containing Cullin 5 and Elongins B and C (Cul5-EloB-EloC) through a novel SOCS (suppressor of cytokine signaling)-box that binds EloC...
  16. Xiao Z, Xiong Y, Zhang W, Tan L, Ehrlich E, Guo D, et al. Characterization of a novel Cullin5 binding domain in HIV-1 Vif. J Mol Biol. 2007;373:541-50 pubmed
    ..a viral-specific BC-box to recruit ElonginB-ElonginC and a novel zinc-binding HCCH motif to recruit Cullin5 (Cul5) to form an E3 ubiquitin ligase targeting A3G for polyubiquitination and subsequently proteasomal degradation...
  17. Mehle A, Thomas E, Rajendran K, Gabuzda D. A zinc-binding region in Vif binds Cul5 and determines cullin selection. J Biol Chem. 2006;281:17259-65 pubmed
    ..virus-1 (HIV-1) Vif overcomes the anti-viral activity of APOBEC3G by targeting it for ubiquitination via a Cullin 5-ElonginB-ElonginC (Cul5-EloBC) E3 ligase...
  18. Thomas J, Matak Vinkovic D, Van Molle I, Ciulli A. Multimeric complexes among ankyrin-repeat and SOCS-box protein 9 (ASB9), ElonginBC, and Cullin 5: insights into the structure and assembly of ECS-type Cullin-RING E3 ubiquitin ligases. Biochemistry. 2013;52:5236-46 pubmed publisher
    ..affinity, and assembly of the quaternary multisubunit complex formed by ASB9, Elongin B, Elongin C (EloBC), and Cullin 5. Here, we describe the application of several biophysical techniques including differential scanning fluorimetry, ..
  19. Albin J, Harris R. Interactions of host APOBEC3 restriction factors with HIV-1 in vivo: implications for therapeutics. Expert Rev Mol Med. 2010;12:e4 pubmed publisher
    ..We also summarise, for the first time, current clinical data on the in vivo effects of APOBEC3 proteins, and survey strategies and progress towards developing therapeutics aimed at the APOBEC3-Vif axis. ..
  20. Zhang W, Du J, Evans S, Yu Y, Yu X. T-cell differentiation factor CBF-? regulates HIV-1 Vif-mediated evasion of host restriction. Nature. 2011;481:376-9 pubmed publisher
    ..HIV-1 Vif forms an E3 ubiquitin ligase complex with cullin 5 (CUL5), elongin B and elongin C , which promotes the polyubiquitination and degradation of APOBEC3 substrates...
  21. Mahrour N, Redwine W, Florens L, Swanson S, Martin Brown S, Bradford W, et al. Characterization of Cullin-box sequences that direct recruitment of Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin ligases. J Biol Chem. 2008;283:8005-13 pubmed publisher
    ..ligases composed of Elongin C-Elongin B-Cullin 2-Rbx1 (Cul2 ubiquitin ligases) or of Elongin C-Elongin B-Cullin 5-Rbx2 (Cul5 ubiquitin ligases)...
  22. Vesterlund M, Zadjali F, Persson T, Nielsen M, Kessler B, Norstedt G, et al. The SOCS2 ubiquitin ligase complex regulates growth hormone receptor levels. PLoS ONE. 2011;6:e25358 pubmed publisher
    ..The demonstration that SOCS2 is an ubiquitin ligase for the GHR unveils the molecular basis for its physiological actions. ..
  23. Byrd P, Stankovic T, McConville C, Smith A, Cooper P, Taylor A. Identification and analysis of expression of human VACM-1, a cullin gene family member located on chromosome 11q22-23. Genome Res. 1997;7:71-5 pubmed
    ..The Hs-VACM-1 gene has homology with the Caenorhabditis elegans gene Ce-cul-5, a member of a family of cullin genes that are involved in cell cycle regulation and that might, when mutated, contribute to tumor progression. ..
  24. Ferguson J, Wu Y, Smith K, Charles P, Powers K, Wang H, et al. ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism. Mol Cell Biol. 2007;27:6407-19 pubmed
    ..We postulate that hydroxylation of ASB4 in normoxia promotes binding to and degradation of substrate protein(s) to modulate vascular differentiation. ..
  25. Zhou X, Evans S, Han X, Liu Y, Yu X. Characterization of the interaction of full-length HIV-1 Vif protein with its key regulator CBF? and CRL5 E3 ubiquitin ligase components. PLoS ONE. 2012;7:e33495 pubmed publisher
    ..a stable complex containing Vif-CBF?-EloB/C was purified in large quantity and shown to bind purified Cullin5 (Cul5)...
  26. Zuo T, Liu D, Lv W, Wang X, Wang J, Lv M, et al. Small-molecule inhibition of human immunodeficiency virus type 1 replication by targeting the interaction between Vif and ElonginC. J Virol. 2012;86:5497-507 pubmed publisher
  27. Kohroki J, Nishiyama T, Nakamura T, Masuho Y. ASB proteins interact with Cullin5 and Rbx2 to form E3 ubiquitin ligase complexes. FEBS Lett. 2005;579:6796-802 pubmed
    ..Here, we show that ASB proteins interact with Cul5-Rbx2 but neither Cul2 nor Rbx1 in cells...
  28. Nie L, Zhao Y, Wu W, Yang Y, Wang H, Sun X. Notch-induced Asb2 expression promotes protein ubiquitination by forming non-canonical E3 ligase complexes. Cell Res. 2011;21:754-69 pubmed publisher
    ..Consistently, ablating the function of Cul1 or Cul5 using DN mutants or siRNAs protected both E2A and Jak2 from Asb2-mediated or Notch-induced degradation...
  29. Dang Y, Siew L, Zheng Y. APOBEC3G is degraded by the proteasomal pathway in a Vif-dependent manner without being polyubiquitylated. J Biol Chem. 2008;283:13124-31 pubmed publisher
    ..It was shown that Vif binds to both A3G and a Cullin 5 ubiquitin-protein isopeptide ligase...
  30. Wang X, Wang X, Zhang H, Lv M, Zuo T, Wu H, et al. Interactions between HIV-1 Vif and human ElonginB-ElonginC are important for CBF-? binding to Vif. Retrovirology. 2013;10:94 pubmed publisher
    ..phenotype by tethering them (APOBEC3DE/3F/3G) to the Vif-CBF-?-ElonginB-ElonginC-Cullin5-Rbx (Vif-CBF-?-EloB-EloC-Cul5-Rbx) E3 complex to induce their proteasomal degradation...
  31. Yu Y, Xiao Z, Ehrlich E, Yu X, Yu X. Selective assembly of HIV-1 Vif-Cul5-ElonginB-ElonginC E3 ubiquitin ligase complex through a novel SOCS box and upstream cysteines. Genes Dev. 2004;18:2867-72 pubmed
    APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is suppressed by HIV-1 Vif, acting through Cul5-ElonginB-ElonginC E3 ubiquitin ligase...
  32. Cai Q, Knight J, Verma S, Zald P, Robertson E. EC5S ubiquitin complex is recruited by KSHV latent antigen LANA for degradation of the VHL and p53 tumor suppressors. PLoS Pathog. 2006;2:e116 pubmed
    ..This motif is necessary for LANA interaction with the Cul5-Elongin BC complex, to promote polyubiquitylation of cellular substrates VHL and p53 in vitro via its amino- and ..
  33. Malim M, Emerman M. HIV-1 accessory proteins--ensuring viral survival in a hostile environment. Cell Host Microbe. 2008;3:388-98 pubmed publisher
    ..Broadly speaking, the HIV-1 accessory proteins modify the local environment within infected cells to ensure viral persistence, replication, dissemination, and transmission. ..
  34. Reingewertz T, Shalev D, Friedler A. Structural disorder in the HIV-1 Vif protein and interaction-dependent gain of structure. Protein Pept Lett. 2010;17:988-98 pubmed
    ..This region includes an HCCH Zn(2+)-binding motif that also mediates Vif binding to Cul5, a protein in the E3 ubiquitin ligase complex...
  35. Malim M. APOBEC proteins and intrinsic resistance to HIV-1 infection. Philos Trans R Soc Lond B Biol Sci. 2009;364:675-87 pubmed publisher
  36. An P, Duggal P, Wang L, O Brien S, Donfield S, Goedert J, et al. Polymorphisms of CUL5 are associated with CD4+ T cell loss in HIV-1 infected individuals. PLoS Genet. 2007;3:e19 pubmed
    ..However, HIV-1 viral infectivity factor (Vif) suppresses Apobec3 activity through the Cullin 5-Elongin B-Elongin C E3 ubiquitin ligase complex...
  37. Guo Y, Dong L, Qiu X, Wang Y, Zhang B, Liu H, et al. Structural basis for hijacking CBF-? and CUL5 E3 ligase complex by HIV-1 Vif. Nature. 2014;505:229-33 pubmed publisher
    ..Here we report a crystal structure of the Vif-CBF-?-CUL5-ELOB-ELOC complex...
  38. Salter J, Lippa G, Belashov I, Wedekind J. Core-binding factor ? increases the affinity between human Cullin 5 and HIV-1 Vif within an E3 ligase complex. Biochemistry. 2012;51:8702-4 pubmed publisher
    HIV-1 Vif masquerades as a receptor for a cellular E3 ligase harboring Elongin B, Elongin C, and Cullin 5 (EloB/C/Cul5) proteins that facilitate degradation of the antiretroviral factor APOBEC3G (A3G)...
  39. Mehle A, Wilson H, Zhang C, Brazier A, McPike M, Pery E, et al. Identification of an APOBEC3G binding site in human immunodeficiency virus type 1 Vif and inhibitors of Vif-APOBEC3G binding. J Virol. 2007;81:13235-41 pubmed
    ..binds APOBEC3G and APOBEC3F and targets these proteins for ubiquitination by forming an E3 ubiquitin ligase with cullin 5 and elongins B and C...
  40. Kwon S, Kim D, Rhee J, Park J, Kim D, Kim D, et al. ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function. BMC Biol. 2010;8:23 pubmed publisher
    ..ASB9 interacts with the creatine kinase system and negatively regulates cell growth. The differential expression and function of ASB9 and ASB9DeltaSOCS may be a key factor in the growth of human cell lines and primary cells. ..
  41. Luo K, Ehrlich E, Xiao Z, Zhang W, Ketner G, Yu X. Adenovirus E4orf6 assembles with Cullin5-ElonginB-ElonginC E3 ubiquitin ligase through an HIV/SIV Vif-like BC-box to regulate p53. FASEB J. 2007;21:1742-50 pubmed
    ..E4orf6 targets p53 for polyubiquitination and proteasomal degradation and is known to form a complex with the Cul5-ElonginB-ElonginC E3 ubiquitin ligase...
  42. Huo Y, Jiang B, Han X, Luo J. [Cell surface adhesion molecules mediated stretch-induced HL-60 cell adhesion to mouse common carotid arteries]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014;30:802-5 pubmed
    ..Stretch can induce HL-60 cell adhesion to carotid arteries in a magnitude-dependent manner. VCAM-1 and P-selectin play some roles in mechanical stretch-induced HL-60 cell adhesion to mouse common carotid arteries. ..
  43. Ma C, Qi Y, Shao L, Liu M, Li X, Tang H. Downregulation of miR-7 upregulates Cullin 5 (CUL5) to facilitate G1/S transition in human hepatocellular carcinoma cells. IUBMB Life. 2013;65:1026-34 pubmed publisher
    ..A tumor suppressor gene, CUL5, was identified as a direct target of miR-7, and CUL-5 is upregulated upon the binding of miR-7 to its 3'UTR...
  44. Harada J, Shevchenko A, Shevchenko A, Pallas D, Berk A. Analysis of the adenovirus E1B-55K-anchored proteome reveals its link to ubiquitination machinery. J Virol. 2002;76:9194-206 pubmed
    ..Lastly, we describe the identification and characterization of two novel E1B-55K interacting factors, importin-alpha 1 and pp32, that may also participate in the functions previously ascribed to E1B-55K and E4-orf6. ..
  45. De Maio F, Rocco C, Aulicino P, Bologna R, Mangano A, Sen L. APOBEC3-mediated editing in HIV type 1 from pediatric patients and its association with APOBEC3G/CUL5 polymorphisms and Vif variability. AIDS Res Hum Retroviruses. 2012;28:619-27 pubmed publisher
    ..The HIV-1 Vif protein has the capacity to counteract APOBEC3 factors by recruiting a CUL5-based ubiquitin ligase complex that determines their proteasomal degradation...
  46. Cardote T, Gadd M, Ciulli A. Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex. Structure. 2017;25:901-911.e3 pubmed publisher
    ..between Cul2 and pVHL-EloBC and identify mutations that contribute toward a selectivity switch for Cul2 versus Cul5 recognition...
  47. Zhu Z, Sun L, Hao R, Jiang H, Qian F, Ye R. Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2017;313:L104-L114 pubmed publisher
  48. Desimmie B, Smith J, Matsuo H, HU W, Pathak V. Identification of a tripartite interaction between the N-terminus of HIV-1 Vif and CBF? that is critical for Vif function. Retrovirology. 2017;14:19 pubmed publisher
    ..for Vif-CBF? interactions, and recently a co-crystal structure of a pentameric complex of HIV-1 Vif, CBF?, Cul5, EloB, and EloC was resolved...
  49. Uematsu K, Okumura F, Tonogai S, Joo Okumura A, Alemayehu D, Nishikimi A, et al. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. J Cell Biol. 2016;215:95-106 pubmed
    ..In this study, we show that Cullin 5-interacting suppressor of cytokine signaling box protein ASB7 ubiquitinates DDA3, a regulator of spindle dynamics,..
  50. Ding G, Chen P, Zhang H, Huang X, Zang Y, Li J, et al. Regulation of Ubiquitin-like with Plant Homeodomain and RING Finger Domain 1 (UHRF1) Protein Stability by Heat Shock Protein 90 Chaperone Machinery. J Biol Chem. 2016;291:20125-35 pubmed publisher
    ..Interestingly, this HSP90 inhibitor-induced UHRF1 degradation is independent of CHIP and CUL5, two previously identified ubiquitin E3 ligases for HSP90 client proteins...
  51. Shirakawa K, Takaori Kondo A, Kobayashi M, Tomonaga M, Izumi T, Fukunaga K, et al. Ubiquitination of APOBEC3 proteins by the Vif-Cullin5-ElonginB-ElonginC complex. Virology. 2006;344:263-6 pubmed
    ..correlates with its ability to bind and ubiquitinate these proteins by a Vif-Cullin5-ElonginB-ElonginC (Vif-BC-Cul5) complex...
  52. Monda J, Scott D, Miller D, Lydeard J, King D, Harper J, et al. Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes. Structure. 2013;21:42-53 pubmed publisher
  53. Lewis S, Willis A, Johnson A, Resau J, Burnatowska Hledin M. Mutational analysis of VACM-1/cul5 exons in cancer cell lines. APMIS. 2011;119:421-30 pubmed publisher
    ..Overexpression of VACM-/cul5 cDNA mutated at the PKA-specific phosphorylation site at Ser730 reversed this phenotype...
  54. Malim M. HIV: Ringside views. Nature. 2014;505:167-8 pubmed publisher
  55. Ghimire Rijal S, Maynard E. Comparative thermodynamic analysis of zinc binding to the His/Cys motif in virion infectivity factor. Inorg Chem. 2014;53:4295-302 pubmed publisher
    ..Degradation of APOBEC3G requires the interaction of Vif with Cul5, the scaffold for an E3 ubiquitin ligase...
  56. Salter J, Morales G, Smith H. Structural insights for HIV-1 therapeutic strategies targeting Vif. Trends Biochem Sci. 2014;39:373-80 pubmed publisher
    ..Vif-mediated protein-protein interactions are excellent targets for a new class of antiretroviral therapeutics to combat AIDS. ..
  57. Schenkova K, Lutz J, Kopp M, Ramos S, Rivero F. MUF1/leucine-rich repeat containing 41 (LRRC41), a substrate of RhoBTB-dependent cullin 3 ubiquitin ligase complexes, is a predominantly nuclear dimeric protein. J Mol Biol. 2012;422:659-673 pubmed publisher
    ..protein containing a leucine-rich repeat and, interestingly, a BC-box that serves as a linker in multicomponent, cullin 5 (Cul5)-based ubiquitin ligases...
  58. Shamay M, Greenway M, Liao G, Ambinder R, Hayward S. De novo DNA methyltransferase DNMT3b interacts with NEDD8-modified proteins. J Biol Chem. 2010;285:36377-86 pubmed publisher
    ..proteins at the size of NEDDylated cullins, and indeed DNMT3b interacted with CUL1, CUL2, CUL3, CUL4A, and CUL5. Moreover, DNMT3b preferentially immunoprecipitated the NEDDylated form of endogenous CUL4A...
  59. Fribourgh J, Nguyen H, Wolfe L, Dewitt D, Zhang W, Yu X, et al. Core binding factor beta plays a critical role by facilitating the assembly of the Vif-cullin 5 E3 ubiquitin ligase. J Virol. 2014;88:3309-19 pubmed publisher
    ..interaction requires an extensive region of Vif spanning most of its amino terminus and zinc finger region, and cullin 5 (Cul5) binding enhances the stability of the Vif-CBF? interaction...
  60. Evans S, SchOn A, Gao Q, Han X, Zhou X, Freire E, et al. HIV-1 Vif N-terminal motif is required for recruitment of Cul5 to suppress APOBEC3. Retrovirology. 2014;11:4 pubmed publisher
    ..The complex is composed of a scaffold protein, Cullin 5 (Cul5), RING-box protein (Rbx), a SOCS box binding protein complex, Elongins B/C (Elo B/C), as well as newly ..
  61. Furukawa M, Ohta T, Xiong Y. Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins. J Biol Chem. 2002;277:15758-65 pubmed
    ..We suggest that RING-E2, rather than cullin-RING, constitutes the catalytic core of the ubiquitin ligase and that one major function of the cullin subunit is to assemble the RING-E2 catalytic core and substrates together. ..
  62. Gilson T, Blanchette P, Ballmann M, Papp T, Pénzes J, Benkő M, et al. Using the E4orf6-Based E3 Ubiquitin Ligase as a Tool To Analyze the Evolution of Adenoviruses. J Virol. 2016;90:7350-7367 pubmed publisher
    ..While most are assembled with Cul5, a few utilize Cul2...
  63. Baxter S, Carlson L, Mayer A, Hall M, Fay M. Granulocytic differentiation of HL-60 promyelocytic leukemia cells is associated with increased expression of Cul5. In Vitro Cell Dev Biol Anim. 2009;45:264-74 pubmed publisher
    ..The ASB-2 protein forms an E3 ubiquitin ligase complex with the proteins, Cul5, regulator of cullin 2 (ROC2), and elongin B and C...
  64. Chiu Y, Greene W. APOBEC3G: an intracellular centurion. Philos Trans R Soc Lond B Biol Sci. 2009;364:689-703 pubmed publisher
    ..LMM A3G opposes the external threat posed by exogenous retroviruses, while HMM A3G complexes oppose the internal threat posed by the retrotransposition of select types of retroelements. ..
  65. Bello N, Lamsoul I, Heuzé M, Metais A, Moreaux G, Calderwood D, et al. The E3 ubiquitin ligase specificity subunit ASB2beta is a novel regulator of muscle differentiation that targets filamin B to proteasomal degradation. Cell Death Differ. 2009;16:921-32 pubmed publisher
    ..Altogether, our results suggest that ASB2beta is involved in muscle differentiation through the targeting of FLNb to destruction by the proteasome. ..