Gene Symbol: CSTF1
Description: cleavage stimulation factor subunit 1
Alias: CstF-50, CstFp50, cleavage stimulation factor subunit 1, CF-1 50 kDa subunit, CSTF 50 kDa subunit, cleavage stimulation factor 50 kDa subunit, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Species: human
Products:     CSTF1

Top Publications

  1. McCracken S, Fong N, Yankulov K, Ballantyne S, Pan G, Greenblatt J, et al. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature. 1997;385:357-61 pubmed
  2. Kleiman F, Manley J. Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50. Science. 1999;285:1576-9 pubmed
  3. Takagaki Y, Manley J. Complex protein interactions within the human polyadenylation machinery identify a novel component. Mol Cell Biol. 2000;20:1515-25 pubmed
    ..These and other data suggest that symplekin may function in assembly of the polyadenylation machinery. ..
  4. Kleiman F, Manley J. The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression. Cell. 2001;104:743-53 pubmed
    ..Together these results indicate a link between mRNA 3' processing and DNA repair and tumor suppression. ..
  5. Fong N, Bentley D. Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD. Genes Dev. 2001;15:1783-95 pubmed
    ..We conclude that the CTD independently stimulates all three major pre-mRNA processing steps and that different regions of the CTD can serve distinct functions in pre-mRNA processing. ..
  6. Moreno Morcillo M, Minvielle Sebastia L, Mackereth C, Fribourg S. Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure. RNA. 2011;17:412-8 pubmed publisher
    ..Together with previous data on the structure of CstF-77, homodimerization of CstF-50 N-terminal domain supports the model in which the functional state of CstF is a heterohexamer. ..
  7. Blanco I, Kuchenbaecker K, Cuadras D, Wang X, Barrowdale D, de Garibay G, et al. Assessing associations between the AURKA-HMMR-TPX2-TUBG1 functional module and breast cancer risk in BRCA1/2 mutation carriers. PLoS ONE. 2015;10:e0120020 pubmed publisher
    ..04-1.15, p = 1.9 x 10(-4) (false discovery rate (FDR)-adjusted p = 0.043). Variation in CSTF1, located next to AURKA, was also found to be associated with breast cancer risk in BRCA2 mutation carriers: ..
  8. Yang W, Hsu P, Yang F, Song J, Varani G. Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals. Nucleic Acids Res. 2017;: pubmed publisher
    ..We further demonstrate that CstF-77 boosts the affinity of the CstF-64 RRM to the RNA targets and CstF-50 fine tunes the ability of the complex to recognize G/U sequences of certain lengths and content...
  9. Murthy K, Manley J. The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation. Genes Dev. 1995;9:2672-83 pubmed
    ..We discuss the significance of these multiple functions and also a possible evolutionary link between yeast and mammalian polyadenylation suggested by the properties and sequence of 160K. ..

More Information


  1. Fonseca D, Baquero J, Murphy M, Aruggoda G, Varriano S, Sapienza C, et al. mRNA Processing Factor CstF-50 and Ubiquitin Escort Factor p97 Are BRCA1/BARD1 Cofactors Involved in Chromatin Remodeling during the DNA Damage Response. Mol Cell Biol. 2018;38: pubmed publisher
    ..This study provides new insights into the roles of RNA processing, BRCA1/BARD1, the Ub pathway, and chromatin structure during DDR. ..
  2. Rüegsegger U, Beyer K, Keller W. Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors. J Biol Chem. 1996;271:6107-13 pubmed
    ..Furthermore, the CstF-CPSF-RNA as well as the CstF-CPSF-PAP-RNA complex are supershifted and stabilized upon the addition of CF Im. ..
  3. Takagaki Y, Manley J. A human polyadenylation factor is a G protein beta-subunit homologue. J Biol Chem. 1992;267:23471-4 pubmed
    ..Possible roles of the transducin repeat, both in CstF function specifically and in other beta-subunit homologues more generally, are discussed. ..
  4. Tsuzuki M, Wu W, Nishikawa H, Hayami R, Oyake D, Yabuki Y, et al. A truncated splice variant of human BARD1 that lacks the RING finger and ankyrin repeats. Cancer Lett. 2006;233:108-16 pubmed
    ..DeltaRIN does not interact with BRCA1, whereas it interacts with and colocalizes with CstF-50 to cytoplasmic dots. Hence, a deletion variant of BARD1 occurs in cells and may play a distinct role with CstF-50. ..
  5. Fontana G, Rigamonti A, Lenzken S, Filosa G, Alvarez R, Calogero R, et al. Oxidative stress controls the choice of alternative last exons via a Brahma-BRCA1-CstF pathway. Nucleic Acids Res. 2017;45:902-914 pubmed publisher
    ..Our findings elucidate a novel regulatory mechanism, distinct from the modulation of transcription elongation by BRM that controls alternative splicing of internal exons. ..
  6. Cevher M, Zhang X, Fernandez S, Kim S, Baquero J, Nilsson P, et al. Nuclear deadenylation/polyadenylation factors regulate 3' processing in response to DNA damage. EMBO J. 2010;29:1674-87 pubmed publisher
  7. Hashizume R, Fukuda M, Maeda I, Nishikawa H, Oyake D, Yabuki Y, et al. The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by a breast cancer-derived mutation. J Biol Chem. 2001;276:14537-40 pubmed
    ..These results suggest that the BRCA1-BARD1 complex contains a ubiquitin ligase activity that is important in prevention of breast and ovarian cancer development. ..
  8. Edwards R, Lee M, Tsutakawa S, Williams R, Nazeer I, Kleiman F, et al. The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50. Biochemistry. 2008;47:11446-56 pubmed publisher
    ..BARD1 architecture and plasticity imparted by the ANK-BRCT linker are suitable to allow the BARD1 C-terminus to act as a hub with multiple binding sites to integrate diverse DNA damage signals directly to RNA polymerase. ..
  9. Takagaki Y, Manley J. A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein. Nature. 1994;372:471-4 pubmed
    ..Our results thus also indicate that components of the complex polyadenylation machinery are conserved from yeast to man. ..
  10. Takagaki Y, MacDonald C, Shenk T, Manley J. The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs. Proc Natl Acad Sci U S A. 1992;89:1403-7 pubmed