CPSF6

Summary

Gene Symbol: CPSF6
Description: cleavage and polyadenylation specific factor 6
Alias: CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7, cleavage and polyadenylation specificity factor subunit 6, CPSF 68 kDa subunit, cleavage and polyadenylation specific factor 6, 68kDa, cleavage and polyadenylation specificity factor 68 kDa subunit, cleavage factor Im complex 68 kDa subunit, pre-mRNA cleavage factor I, 68kD subunit, pre-mRNA cleavage factor Im (68kD), pre-mRNA cleavage factor Im 68 kDa subunit, protein HPBRII-4/7
Species: human
Products:     CPSF6

Top Publications

  1. De Iaco A, Santoni F, Vannier A, Guipponi M, Antonarakis S, Luban J. TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm. Retrovirology. 2013;10:20 pubmed publisher
    ..Yet, an equal number of investigators have failed to obtain evidence that supports this model. Here, a series of experiments were performed to better elucidate the mechanism by which TNPO3 promotes HIV-1 infectivity...
  2. Yang Q, Coseno M, Gilmartin G, DOUBLIE S. Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping. Structure. 2011;19:368-77 pubmed publisher
    ..The intrinsic ability of CFI(m) to direct RNA looping may provide a mechanism for its function in the regulation of alternative poly(A) site selection. ..
  3. Rasaiyaah J, Tan C, Fletcher A, Price A, Blondeau C, Hilditch L, et al. HIV-1 evades innate immune recognition through specific cofactor recruitment. Nature. 2013;503:402-405 pubmed publisher
    ..HIV-1 capsid mutants N74D and P90A, which are impaired for interaction with cofactors cleavage and polyadenylation specificity factor subunit 6 (CPSF6) and cyclophilins (Nup358 and CypA), respectively, cannot replicate in primary ..
  4. Ruepp M, Aringhieri C, Vivarelli S, Cardinale S, Paro S, Schumperli D, et al. Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export. Mol Biol Cell. 2009;20:5211-23 pubmed publisher
    ..These results reveal a novel function for the pre-mRNA 3' end processing factor CF I(m)68 in mRNA export. ..
  5. Price A, Fletcher A, Schaller T, Elliott T, Lee K, Kewalramani V, et al. CPSF6 defines a conserved capsid interface that modulates HIV-1 replication. PLoS Pathog. 2012;8:e1002896 pubmed publisher
    ..Mutation of the interface prevents binding to and restriction by CPSF6-358, a truncated cytosolic form of the RNA processing factor, cleavage and polyadenylation specific factor 6 (..
  6. Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino S. Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit. Mol Biol Cell. 2007;18:1282-92 pubmed
    ..We show that CFIm68 colocalizes with bromouridine, RNA polymerase II, and the splicing factor SC35...
  7. Dettwiler S, Aringhieri C, Cardinale S, Keller W, Barabino S. Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization. J Biol Chem. 2004;279:35788-97 pubmed
    ..However, CF I(m)68 does not concentrate in splicing speckles but in foci that partially colocalize with paraspeckles, a subnuclear component in which other proteins involved in transcriptional control and RNA processing have been found. ..
  8. Rüegsegger U, Beyer K, Keller W. Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors. J Biol Chem. 1996;271:6107-13 pubmed
    ..Furthermore, the CstF-CPSF-RNA as well as the CstF-CPSF-PAP-RNA complex are supershifted and stabilized upon the addition of CF Im. ..
  9. Henning M, Dubose B, Burse M, Aiken C, Yamashita M. In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog. 2014;10:e1003868 pubmed publisher
    The host protein CPSF6 possesses a domain that can interact with the HIV-1 capsid (CA) protein. CPSF6 has been implicated in regulating HIV-1 nuclear entry...

More Information

Publications77

  1. Hilditch L, Towers G. A model for cofactor use during HIV-1 reverse transcription and nuclear entry. Curr Opin Virol. 2014;4:32-6 pubmed publisher
    ..part of the viral lifecycle by utilising cyclophilin A (CypA), cleavage and polyadenylation specificity factor-6 (CPSF6), Nup358 and TNPO3 to orchestrate a coordinated process of DNA synthesis, capsid uncoating and integration ..
  2. Lee K, Ambrose Z, Martin T, Oztop I, Mulky A, Julias J, et al. Flexible use of nuclear import pathways by HIV-1. Cell Host Microbe. 2010;7:221-33 pubmed publisher
    ..Here we identify a fragment of the cleavage and polyadenylation factor 6, CPSF6, as a potent inhibitor of HIV-1 infection...
  3. Hori T, Takeuchi H, Saito H, Sakuma R, Inagaki Y, Yamaoka S. A carboxy-terminally truncated human CPSF6 lacking residues encoded by exon 6 inhibits HIV-1 cDNA synthesis and promotes capsid disassembly. J Virol. 2013;87:7726-36 pubmed publisher
    ..studies showed that a C-terminally truncated cytosolic form of cleavage and polyadenylation-specific factor 6 (CPSF6-358) inhibits HIV-1 infection through interference with HIV-1 trafficking to the nucleus...
  4. Shah V, Aiken C. HIV Nuclear Entry: Clearing the Fog. Viruses. 2010;2:1190-4 pubmed publisher
    ..The identification of mutations in the viral capsid that alter the virus's dependence on host cell nucleoporins represents an important advance in this poorly understood stage of the virus life cycle. ..
  5. Lee K, Mulky A, Yuen W, Martin T, Meyerson N, Choi L, et al. HIV-1 capsid-targeting domain of cleavage and polyadenylation specificity factor 6. J Virol. 2012;86:3851-60 pubmed publisher
    The antiviral factor CPSF6-358 restricts human immunodeficiency virus type 1 (HIV-1) infection through an interaction with capsid (CA), preventing virus nuclear entry and integration...
  6. Ambrose Z, Lee K, Ndjomou J, Xu H, Oztop I, Matous J, et al. Human immunodeficiency virus type 1 capsid mutation N74D alters cyclophilin A dependence and impairs macrophage infection. J Virol. 2012;86:4708-14 pubmed publisher
    The antiviral factor CPSF6-358 interferes with the nuclear entry of human immunodeficiency virus type 1 (HIV-1). HIV-1 acquires resistance to CPSF6-358 through the N74D mutation of the capsid (CA), which alters its nuclear entry pathway...
  7. Fricke T, Brandariz Nuñez A, Wang X, Smith A, Diaz Griffero F. Human cytosolic extracts stabilize the HIV-1 core. J Virol. 2013;87:10587-97 pubmed publisher
    ..with previous observations using the fate-of-the-capsid assay, we also demonstrated the ability of recombinant CPSF6 to stabilize HIV-1 CA-NC complexes...
  8. Price A, Jacques D, McEwan W, Fletcher A, Essig S, Chin J, et al. Host cofactors and pharmacologic ligands share an essential interface in HIV-1 capsid that is lost upon disassembly. PLoS Pathog. 2014;10:e1004459 pubmed publisher
    ..formed exclusively within CA hexamers mediates binding to linear epitopes within cellular cofactors NUP153 and CPSF6, and is competed for by the antiretroviral compounds PF74 and BI-2...
  9. Fricke T, Valle Casuso J, White T, Brandariz Nuñez A, Bosche W, Reszka N, et al. The ability of TNPO3-depleted cells to inhibit HIV-1 infection requires CPSF6. Retrovirology. 2013;10:46 pubmed publisher
    ..This work explores the role of cleavage and polyadenylation specificity factor subunit 6 (CPSF6) in the ability of TNPO3-depleted cells to inhibit HIV-1 infection...
  10. Rüegsegger U, Blank D, Keller W. Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Mol Cell. 1998;1:243-53 pubmed
  11. Vissoci J, de Oliveira L, Gafaar T, Haglund M, Mvungi M, Mmbaga B, et al. Cross-cultural adaptation and psychometric properties of the MMSE and MoCA questionnaires in Tanzanian Swahili for a traumatic brain injury population. BMC Neurol. 2019;19:57 pubmed publisher
    ..MMSE does not appear to be an appropriate tool in this patient group, but its positive correlations with MoCA and cFIM indicate similar theoretical concepts...
  12. Zhao J, Liang J, Xue H, Shen S, Chen J, Tang Y, et al. The genetics and clinical characteristics of children morphologically diagnosed as acute promyelocytic leukemia. Leukemia. 2018;: pubmed publisher
    ..In the eligible 13 RNA samples without any RARA rearrangement at diagnosis, one case each with CPSF6-RARG, NPM1-CCDC28A, and TBC1D15-RAB21 and two cases with a TBL1XR1-RARB fusion were discovered...
  13. Chu Y, Elrod N, Wang C, Li L, Chen T, Routh A, et al. Nudt21 regulates the alternative polyadenylation of Pak1 and is predictive in the prognosis of glioblastoma patients. Oncogene. 2019;: pubmed publisher
    ..Moreover, although CFIm25 was initially identified as biochemically associated with both CFIm59 and CFIm68, we observed three CFIm distinct subcomplexes exist and CFIm59 protein level is dependent on Nudt21 expression in ..
  14. Dong Y, Fasina O, Pintel D. Minute Virus of Canines NP1 Protein Interacts with the Cellular Factor CPSF6 To Regulate Viral Alternative RNA Processing. J Virol. 2019;93: pubmed publisher
    ..NP1, but not processing mutants of NP1, bound to MVC RNA directly. The cellular RNA processing factor CPSF6 interacted with NP1 in transfected cells and participated with NP1 to modulate its effects...
  15. Ospina Villa J, García Contreras J, Rosas Trigueros J, Ramirez Moreno E, López Camarillo C, Zamora López B, et al. Importance of amino acids Leu135 and Tyr236 for the interaction between EhCFIm25 and RNA: a molecular dynamics simulation study. J Mol Model. 2018;24:202 pubmed publisher
    The CFIm25 subunit of the heterotetrameric cleavage factor Im (CFIm) is a critical factor in the formation of the poly(A) tail at mRNA 3' end, regulating the recruitment of polyadenylation factors, poly(A) site selection, and cleavage/..
  16. Sasado T, Kondoh H, Furutani Seiki M, Naruse K. Mutation in cpsf6/CFIm68 (Cleavage and Polyadenylation Specificity Factor Subunit 6) causes short 3'UTRs and disturbs gene expression in developing embryos, as revealed by an analysis of primordial germ cell migration using the medaka mutant naruto. PLoS ONE. 2017;12:e0172467 pubmed publisher
    ..A fine genetic linkage mapping and genome sequencing showed the nar gene encodes Cleavage and Polyadenylation Specificity Factor subunit 6 (CPSF6/CFIm68)...
  17. Selyutina A, Bulnes Ramos A, Diaz Griffero F. Binding of host factors to stabilized HIV-1 capsid tubes. Virology. 2018;523:1-5 pubmed publisher
    ..We validated the reliability and specificity of this novel assay by testing the capsid binding ability of TRIMCyp, CPSF6 and MxB with their corresponding controls...
  18. Buffone C, Martínez López A, Fricke T, Opp S, Severgnini M, Cifola I, et al. Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells. J Virol. 2018;92: pubmed publisher
    ..The mutant capsids also lost the ability to interact with Nup153 and CPSF6. The use of small molecules PF74 and BI-2 prevented the interaction of FG-containing nucleoporins (Nups), such as ..
  19. Sowalsky A, Xia Z, Wang L, Zhao H, Chen S, Bubley G, et al. Whole transcriptome sequencing reveals extensive unspliced mRNA in metastatic castration-resistant prostate cancer. Mol Cancer Res. 2015;13:98-106 pubmed publisher
    ..Using quantitative PCR (qPCR), a series of genes (AR, KLK2, KLK3, STEAP2, CPSF6, and CDK19) were confirmed to have a greater proportion of unspliced RNA in CRPC specimens than in normal prostate ..
  20. Achuthan V, Perreira J, Sowd G, Puray Chavez M, McDougall W, Paulucci Holthauzen A, et al. Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA Integration. Cell Host Microbe. 2018;24:392-404.e8 pubmed publisher
    ..periphery provides the architectural basis for integration site selection, with viral capsid-binding host cofactor CPSF6 and viral integrase-binding cofactor LEDGF/p75 contributing to selection of individual sites...
  21. Ning J, Zhong Z, Fischer D, Harris G, Watkins S, Ambrose Z, et al. Truncated CPSF6 Forms Higher-Order Complexes That Bind and Disrupt HIV-1 Capsid. J Virol. 2018;92: pubmed publisher
    Cleavage and polyadenylation specificity factor 6 (CPSF6) is a human protein that binds HIV-1 capsid and mediates nuclear transport and integration targeting of HIV-1 preintegration complexes...
  22. Qiu Z, Wang F, Hong Y, Zhang J, Tang H, Li X, et al. Clinical Predictors of Neurological Outcome within 72?h after Traumatic Cervical Spinal Cord Injury. Sci Rep. 2016;6:38909 pubmed publisher
    ..024, OR?=?0.241) was related to complete SCI. Cases with compression flexion injury mechanism (CFIM), most significantly related to motor complete SCIs by univariate analysis (P?=?0...
  23. Redis R, Vela L, Lu W, Ferreira de Oliveira J, Ivan C, Rodriguez Aguayo C, et al. Allele-Specific Reprogramming of Cancer Metabolism by the Long Non-coding RNA CCAT2. Mol Cell. 2016;61:520-534 pubmed publisher
    ..manner by binding the Cleavage Factor I (CFIm) complex with distinct affinities for the two subunits (CFIm25 and CFIm68)...
  24. Leahey M, Wright L. Application of the Calgary Family Assessment and Intervention Models: Reflections on the Reciprocity Between the Personal and the Professional. J Fam Nurs. 2016;22:450-459 pubmed
    Much has been written about the global implementation of the Calgary Family Assessment and Intervention Models (CFAM/CFIM) and the application of these practice models in various clinical settings...
  25. Bhargava A, Lahaye X, Manel N. Let me in: Control of HIV nuclear entry at the nuclear envelope. Cytokine Growth Factor Rev. 2018;40:59-67 pubmed publisher
    ..entry into the nucleus, including the viral capsid, integrase, the central viral DNA flap, and the host proteins CPSF6, TNPO3, Nucleoporins, SUN1, SUN2, Cyclophilin A and MX2...
  26. Fricke T, Diaz Griffero F. HIV-1 Capsid Stabilization Assay. Methods Mol Biol. 2016;1354:39-47 pubmed publisher
    ..We also found that purified CPSF6 (1-321) protein stabilizes in vitro-assembled HIV-1 CA-NC complexes (Fricke et al., J Virol 87:10587-10597, 2013)...
  27. Tang H, Hu Y, Chen C, Xia B, ZIRIN J, Yuan M, et al. The TORC1-Regulated CPA Complex Rewires an RNA Processing Network to Drive Autophagy and Metabolic Reprogramming. Cell Metab. 2018;27:1040-1054.e8 pubmed publisher
    ..We show that TORC1 signaling inhibits CDK8 and DOA kinases, which directly phosphorylate CPSF6, a component of the CPA complex...
  28. Hu B, Yang Y, Huang Y, Zhu Y, Lu Z. POSTAR: a platform for exploring post-transcriptional regulation coordinated by RNA-binding proteins. Nucleic Acids Res. 2017;45:D104-D114 pubmed publisher
    ..were able to obtain novel insights into post-transcriptional regulation, such as the putative association between CPSF6 binding, RNA structural domains, and Li-Fraumeni syndrome SNPs...
  29. Binothman N, Hachim I, Lebrun J, Ali S. CPSF6 is a Clinically Relevant Breast Cancer Vulnerability Target: Role of CPSF6 in Breast Cancer. EBioMedicine. 2017;21:65-78 pubmed publisher
    ..Here, we describe a novel tumor promoting function for the Cleavage and Polyadenylation Factor-6 (CPSF6)...
  30. Hudson S, McNally L, McNally M. Evidence that a threshold of serine/arginine-rich (SR) proteins recruits CFIm to promote rous sarcoma virus mRNA 3' end formation. Virology. 2016;498:181-191 pubmed publisher
    ..polyadenylation when the distance to the PAS is decreased, and that SR protein binding sites promote binding of the polyadenylation factor cleavage factor I (CFIm) to the weak PAS. These observations may be relevant for cellular PASs.
  31. Bulli L, Apolonia L, Kutzner J, Pollpeter D, Goujon C, Herold N, et al. Complex Interplay between HIV-1 Capsid and MX2-Independent Alpha Interferon-Induced Antiviral Factors. J Virol. 2016;90:7469-7480 pubmed publisher
    ..allow escape from inhibition by MX2 and the truncated version of cleavage and polyadenylation specific factor 6 (CPSF6), as well as the cyclophilin A (CypA)-binding loop mutation P90A, all increase sensitivity to IFN-α-mediated ..
  32. de Vries H, Rüegsegger U, Hubner W, Friedlein A, Langen H, Keller W. Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. EMBO J. 2000;19:5895-904 pubmed
    ..hClp1 interacts with CF I(m) and the cleavage and polyadenylation specificity factor CPSF, suggesting that it bridges these two 3' end processing factors within the cleavage complex. ..
  33. Di Nunzio F. New insights in the role of nucleoporins: a bridge leading to concerted steps from HIV-1 nuclear entry until integration. Virus Res. 2013;178:187-96 pubmed publisher
    ..In particular, it will be reviewed both what is known and what is speculated about the link between HIV translocation through the nuclear pore and the proviral integration in the host chromatin. ..
  34. Goff S. HIV: Slipping under the radar. Nature. 2013;503:352-3 pubmed publisher
  35. Kim S, Yamamoto J, Chen Y, Aida M, Wada T, Handa H, et al. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes Cells. 2010;15:1003-13 pubmed publisher
    ..iii) However, the structurally related CFIm68 and CFIm59 are functionally redundant and (iv) CFIm68 appears to have a higher specific activity...
  36. Sagawa F, Ibrahim H, Morrison A, Wilusz C, Wilusz J. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J. 2011;30:3994-4005 pubmed publisher
    ..Collectively, these data suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3' end processing with other aspects of nuclear mRNA maturation. ..
  37. Awasthi S, Alwine J. Association of polyadenylation cleavage factor I with U1 snRNP. RNA. 2003;9:1400-9 pubmed
    ..Because mAb 2.73 has been used in a number of previous studies related to the U1 snRNP and the U1 70K protein, the precipitation of CF Im must be considered in evaluating past and future data based on the use of mAb 2.73. ..
  38. Venkataraman K, Brown K, Gilmartin G. Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition. Genes Dev. 2005;19:1315-27 pubmed
    ..The recognition of three distinct sequence elements by CFI(m), CPSF, and CstF suggests that vertebrate poly(A) site definition is mechanistically more similar to that of yeast and plants than anticipated. ..
  39. Maelfait J, Seiradake E, Rehwinkel J. Keeping your armour intact: how HIV-1 evades detection by the innate immune system: HIV-1 capsid controls detection of reverse transcription products by the cytosolic DNA sensor cGAS. Bioessays. 2014;36:649-57 pubmed publisher
    ..This in turn induces a strong host response. Here, we discuss these findings in the context of recent developments in innate immunity and consider the implications for disease control and vaccine design. ..
  40. Chen N, Zhou L, Gane P, Opp S, Ball N, Nicastro G, et al. HIV-1 capsid is involved in post-nuclear entry steps. Retrovirology. 2016;13:28 pubmed publisher
    ..Furthermore C-A1 increased binding of CPSF6, a nuclear protein, to capsid...
  41. Li H, Tong S, Li X, Shi H, Ying Z, Gao Y, et al. Structural basis of pre-mRNA recognition by the human cleavage factor Im complex. Cell Res. 2011;21:1039-51 pubmed publisher
  42. Chu M, Ji X, Chen W, Zhang R, Sun C, Wang T, et al. A genome-wide association study identifies susceptibility loci of silica-related pneumoconiosis in Han Chinese. Hum Mol Genet. 2014;23:6385-94 pubmed publisher
  43. Dharan A, Talley S, Tripathi A, Mamede J, Majetschak M, Hope T, et al. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog. 2016;12:e1005700 pubmed publisher
    ..that the relocalization of Nup358 in response to CA is dependent on cleavage protein and polyadenylation factor 6 (CPSF6), but independent of cyclophilin A...
  44. Shimazu T, Horinouchi S, Yoshida M. Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3'-end processing. J Biol Chem. 2007;282:4470-8 pubmed
    ..These results suggest that CBP and HDACs regulate the 3'-end processing machinery and modulate the localization of PAP through the acetylation and deacetylation cycle. ..
  45. Millevoi S, Loulergue C, Dettwiler S, Karaa S, Keller W, Antoniou M, et al. An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. EMBO J. 2006;25:4854-64 pubmed
    ..These results therefore uncover a direct role of the U2AF 65/CF I(m) 59 interaction in the functional coordination of splicing and 3' end processing. ..
  46. Wilusz J. Putting an 'End' to HIV mRNAs: capping and polyadenylation as potential therapeutic targets. AIDS Res Ther. 2013;10:31 pubmed publisher
    ..This review describes these post-transcriptional novelties of HIV gene expression as well as their implications in viral biology and as possible targets for therapeutic intervention. ..
  47. Brown K, Gilmartin G. A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im. Mol Cell. 2003;12:1467-76 pubmed
    ..These data provide evidence for a mechanism for the regulation of poly(A) site selection by a basal pre-mRNA 3' processing factor. ..
  48. Le Sage V, Mouland A, Valiente Echeverría F. Roles of HIV-1 capsid in viral replication and immune evasion. Virus Res. 2014;193:116-29 pubmed publisher
    ..The fact that CA engages in a number of different protein-protein interactions with the host makes it an interesting target for the development of new potent antiviral agents. ..
  49. Saito A, Ferhadian D, Sowd G, Serrao E, Shi J, Halambage U, et al. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol. 2016;90:5808-5823 pubmed publisher
    ..antiviral compound, directly binds to the capsid (CA) protein at a site also utilized by host cell proteins CPSF6 and NUP153...
  50. Rasheedi S, Shun M, Serrao E, Sowd G, Qian J, Hao C, et al. The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes. J Biol Chem. 2016;291:11809-19 pubmed publisher
    ..This requires cleavage and polyadenylation specificity factor 6 (CPSF6), a cellular protein involved in pre-mRNA 3' end processing that binds HIV-1 capsid and connects HIV-1 ..
  51. Sowd G, Serrao E, Wang H, Wang W, Fadel H, Poeschla E, et al. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A. 2016;113:E1054-63 pubmed publisher
    ..targeting, but the influence of another capsid binding protein, cleavage and polyadenylation specificity factor 6 (CPSF6), has not been reported...
  52. Shi J, Zhou J, Halambage U, Shah V, Burse M, Wu H, et al. Compensatory substitutions in the HIV-1 capsid reduce the fitness cost associated with resistance to a capsid-targeting small-molecule inhibitor. J Virol. 2015;89:208-19 pubmed publisher
    ..5Mut substitutions also reduced the binding of the host protein CPSF6 to assembled CA complexes in vitro and permitted infection of cells expressing the inhibitory protein CPSF6-358...
  53. Zhou J, Price A, Halambage U, James L, Aiken C. HIV-1 Resistance to the Capsid-Targeting Inhibitor PF74 Results in Altered Dependence on Host Factors Required for Virus Nuclear Entry. J Virol. 2015;89:9068-79 pubmed publisher
    ..was not significantly perturbed by binding of PF74; rather, the mutations inhibited capsid interactions with CPSF6 and Nup153 and altered HIV-1 dependence on these host factors and on TNPO3...
  54. Hardy J, Norbury C. Cleavage factor Im (CFIm) as a regulator of alternative polyadenylation. Biochem Soc Trans. 2016;44:1051-7 pubmed publisher
    ..The cleavage factor Im (CFIm) complex is a core component of the mammalian cleavage machinery, and the observation that its depletion causes ..
  55. Saito A, Henning M, Serrao E, Dubose B, Teng S, Huang J, et al. Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo. J Virol. 2016;90:6918-6935 pubmed publisher
    b>Cleavage and polyadenylation specificity factor subunit 6 (CPSF6), a host factor that interacts with the HIV-1 capsid (CA) protein, is implicated in diverse functions during the early part of the HIV-1 life cycle, including uncoating, ..
  56. Ambrose Z, Aiken C. HIV-1 uncoating: connection to nuclear entry and regulation by host proteins. Virology. 2014;454-455:371-9 pubmed publisher
    ..Understanding uncoating will be valuable toward developing novel antiretroviral therapies for HIV-infected individuals. ..
  57. Burse M, Shi J, Aiken C. Cyclophilin A potentiates TRIM5? inhibition of HIV-1 nuclear import without promoting TRIM5? binding to the viral capsid. PLoS ONE. 2017;12:e0182298 pubmed publisher
    ..that the ability of CypA to inhibit HIV-1 infection is abolished by amino acid substitutions within the conserved CPSF6-binding surface in CA...
  58. Chin C, Perreira J, Savidis G, Portmann J, Aker A, Feeley E, et al. Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration. Cell Rep. 2015;13:1717-31 pubmed publisher
    ..We also find that CA's interaction with the host polyadenylation factor, CPSF6, enhances nuclear entry and potentiates HIV-1's depth of nuclear invasion, potentially aiding the virus's ..
  59. Martin G, Gruber A, Keller W, Zavolan M. Genome-wide analysis of pre-mRNA 3' end processing reveals a decisive role of human cleavage factor I in the regulation of 3' UTR length. Cell Rep. 2012;1:753-63 pubmed publisher
  60. Gruber A, Martin G, Keller W, Zavolan M. Cleavage factor Im is a key regulator of 3' UTR length. RNA Biol. 2012;9:1405-12 pubmed publisher
    ..More specifically, we demonstrate that the loss-of-function of CF Im 68 and CF Im 25 but not of CF Im 59 leads to a transcriptome-wide increase in the use of proximal polyadenylation sites in HEK293 cells. ..
  61. Naganuma T, Nakagawa S, Tanigawa A, Sasaki Y, Goshima N, Hirose T. Alternative 3'-end processing of long noncoding RNA initiates construction of nuclear paraspeckles. EMBO J. 2012;31:4020-34 pubmed publisher
    ..An in vitro 3'-end processing assay revealed that HNRNPK arrested binding of the CPSF6-NUDT21 (CFIm) complex in the vicinity of the alternative polyadenylation site of NEAT1_1...
  62. Fricke T, Buffone C, Opp S, Valle Casuso J, Diaz Griffero F. BI-2 destabilizes HIV-1 cores during infection and Prevents Binding of CPSF6 to the HIV-1 Capsid. Retrovirology. 2014;11:120 pubmed publisher
    ..that BI-2 destabilizes the HIV-1 core during infection, and prevents the binding of the cellular factor CPSF6 to the HIV-1 core...
  63. Zhu Y, Wang X, Forouzmand E, Jeong J, Qiao F, Sowd G, et al. Molecular Mechanisms for CFIm-Mediated Regulation of mRNA Alternative Polyadenylation. Mol Cell. 2018;69:62-74.e4 pubmed publisher
    ..encode many poorly characterized SR-like proteins, including subunits of the mRNA 3'-processing factor CFIm, CFIm68 and CFIm59. Here we demonstrate that CFIm functions as an enhancer-dependent activator of mRNA 3' processing...
  64. Fricke T, White T, Schulte B, de Souza Aranha Vieira D, Dharan A, Campbell E, et al. MxB binds to the HIV-1 core and prevents the uncoating process of HIV-1. Retrovirology. 2014;11:68 pubmed publisher
    ..Overall our work establishes that MxB binds to the HIV-1 core and inhibits the uncoating process of HIV-1. Moreover, we demonstrated that HIV-1 restriction by MxB requires capsid binding and oligomerization. ..
  65. Lad L, Clancy S, Koditek D, Wong M, Jin D, Niedziela Majka A, et al. Functional label-free assays for characterizing the in vitro mechanism of action of small molecule modulators of capsid assembly. Biochemistry. 2015;54:2240-8 pubmed publisher
    ..characterize the interaction between capsid and the host protein cleavage and polyadenylation specific factor 6 (CPSF6)...
  66. Tedbury P, Freed E. HIV-1 gag: an emerging target for antiretroviral therapy. Curr Top Microbiol Immunol. 2015;389:171-201 pubmed publisher
    ..In this review, we outline the advances made in targeting the Gag protein and its mature products, particularly capsid and nucleocapsid, and highlight possible targets for future pharmacological intervention. ..
  67. Bhattacharya A, Alam S, Fricke T, Zadrozny K, Sedzicki J, Taylor A, et al. Structural basis of HIV-1 capsid recognition by PF74 and CPSF6. Proc Natl Acad Sci U S A. 2014;111:18625-30 pubmed publisher
    ..a drug that inhibits HIV-1 infection, as well as host proteins cleavage and polyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer with at least 10-fold higher affinities compared with ..
  68. Peng K, Muranyi W, Glass B, Laketa V, Yant S, Tsai L, et al. Quantitative microscopy of functional HIV post-entry complexes reveals association of replication with the viral capsid. elife. 2014;3:e04114 pubmed publisher
    ..the CA-targeted inhibitor PF74 exhibits a bimodal mechanism, blocking RTC/PIC association with the host factor CPSF6 and nuclear entry at low, and abrogating reverse transcription at high concentrations...