CPSF3

Summary

Gene Symbol: CPSF3
Description: cleavage and polyadenylation specific factor 3, 73kDa
Alias: CPSF-73, CPSF73, cleavage and polyadenylation specificity factor subunit 3, mRNA 3'-end-processing endonuclease CPSF-73
Species: human

Top Publications

  1. pmc Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression
    Marco A Calzado
    Departamento de Biologia Celular, Fisiologia e Inmunologia, Facultad de Medicina, Universidad de Cordoba, Cordoba, Spain
    J Virol 78:6846-54. 2004
  2. pmc Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor
    J Zhao
    Department of Molecular Biology, School of Medicine, Tufts University, Boston, Massachusetts, USA
    Mol Cell Biol 19:7733-40. 1999
  3. ncbi The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins
    S M Barabino
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Genes Dev 11:1703-16. 1997
  4. ncbi Restoration of both structure and function to a defective poly(A) site by in vitro selection
    B R Graveley
    Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, Stafford Hall, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 271:33654-63. 1996
  5. ncbi An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF
    Barbara J Elliott
    Plant Physiology Biochemistry Molecular Biology Program, Department of Agronomy, University of Kentucky, Lexington, KY 40546 0091, USA
    Plant Mol Biol 51:373-84. 2003
  6. ncbi Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA
    J Zhao
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111 1800, USA
    J Biol Chem 272:10831-8. 1997
  7. pmc The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3'-end processing
    Mohamed A Ghazy
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
    Mol Cell Biol 29:2296-307. 2009
  8. ncbi The 73 kD subunit of the cleavage and polyadenylation specificity factor (CPSF) complex affects reproductive development in Arabidopsis
    Ruqiang Xu
    Department of Botany, Miami University, Oxford, OH 45056, USA
    Plant Mol Biol 61:799-815. 2006
  9. pmc Serotype IX, a Proposed New Streptococcus agalactiae Serotype
    Hans Christian Slotved
    Neisseria and Streptococcus Reference Laboratory, Department for Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark
    J Clin Microbiol 45:2929-36. 2007
  10. pmc N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and alpha CaMKII mRNA polyadenylation at synapses
    Yi Shuian Huang
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    EMBO J 21:2139-48. 2002

Scientific Experts

  • Zbigniew Dominski
  • M A Ibrahim
  • K Yankulov
  • Hans Christian Slotved
  • Kevin Ryan
  • HAROLD MARTINSON
  • Catherine Papin
  • Narayan Sahoo
  • Suryadevara S Rao
  • Xin Heng
  • C Guthrie
  • I Callebaut
  • Dmytro M Hovorun
  • Ilse Hofmann
  • W Keller
  • S M Barabino
  • G M Gilmartin
  • Bernhard Dichtl
  • James Manley
  • Joel Richter
  • Liang Tong
  • JOAN STEITZ
  • Daniel Schoenberg
  • Robert Martienssen
  • James L Manley
  • Joel D Richter
  • Xiao cui Yang
  • Joan A Steitz
  • Ramin Shiekhattar
  • Robert A Martienssen
  • Qingshun Li
  • Francis Millett
  • Mindy Steiniger
  • Qingshun Q Li
  • Clara Collart
  • William F Marzluff
  • Ruqiang Xu
  • Hongwei Zhao
  • Jong Heon Kim
  • Qingshun Quinn Li
  • Nikolay G Kolev
  • Corey R Mandel
  • Georges Martin
  • Denghui Xing
  • Georg Kochs
  • Vasupradha Vethantham
  • Duy Khanh Phung
  • Marco A Calzado
  • Todd R Albrecht
  • C L Li
  • Cecilia Johansson
  • Ivan Sabath
  • Edmond Chipumuro
  • Bijàn Mir-Montazeri
  • Yoko Tacahashi
  • Min Kook Kang
  • Andrea Conidi
  • Brandon D Burch
  • Jian qiang Kong
  • Rakesh S Laishram
  • Yuming Wang
  • Wei Wang
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  • Shamima Akhter
  • Itai Dov Tkacz
  • Sabine Steidle
  • Yi Shuian Huang
  • Hélène Kiefer
  • Orit Rozenblatt-Rosen
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  • Z H Zhu
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  • Richard A Anderson
  • Mohamed A Ghazy
  • Lance R Thurlow
  • Uwe Kuhn
  • Adolfo Garcia-Sastre
  • Luis Martinez-Sobrido
  • Monika Garas
  • Mingxue Cui
  • Helois E Radford
  • A Jenny
  • Stephanie D Himpsl
  • Michael L Gonzales
  • Fanrong Kong
  • Danny Huylebroeck
  • Xiaoyuan He
  • Claire Moore
  • Syuzo Kaneko

Detail Information

Publications115 found, 100 shown here

  1. pmc Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression
    Marco A Calzado
    Departamento de Biologia Celular, Fisiologia e Inmunologia, Facultad de Medicina, Universidad de Cordoba, Cordoba, Spain
    J Virol 78:6846-54. 2004
    ..increases the expression of the cleavage and polyadenylation specificity factor (CPSF) 73-kDa subunit (CPSF3) without affecting the expression of the 160- and 100-kDa subunits of the CPSF complex...
  2. pmc Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor
    J Zhao
    Department of Molecular Biology, School of Medicine, Tufts University, Boston, Massachusetts, USA
    Mol Cell Biol 19:7733-40. 1999
    ..The involvement of Pta1 in both steps of mRNA 3'-end formation supports the conclusion that CF II is the functional homolog of CPSF...
  3. ncbi The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins
    S M Barabino
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Genes Dev 11:1703-16. 1997
    ..Efficient polyadenylation activity can be restored by the addition of purified polyadenylation factor I (PF I). We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit...
  4. ncbi Restoration of both structure and function to a defective poly(A) site by in vitro selection
    B R Graveley
    Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, Stafford Hall, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 271:33654-63. 1996
    ..These results indicate that both the sequence and structural context of the AAUAAA hexamer contribute to poly(A) site recognition by CPSF...
  5. ncbi An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF
    Barbara J Elliott
    Plant Physiology Biochemistry Molecular Biology Program, Department of Agronomy, University of Kentucky, Lexington, KY 40546 0091, USA
    Plant Mol Biol 51:373-84. 2003
    ..The interaction with PAP thus indicates that the plant CPSF100 polypeptide is likely part of the 3'-end processing machinery, but suggests that this complex may function differently in plants than it does in mammals and yeast...
  6. ncbi Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA
    J Zhao
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111 1800, USA
    J Biol Chem 272:10831-8. 1997
    ..These results provide additional evidence that certain features of the molecular mechanism of mRNA 3'-end formation are conserved between yeast and mammals, but also highlight unexpected differences...
  7. pmc The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3'-end processing
    Mohamed A Ghazy
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
    Mol Cell Biol 29:2296-307. 2009
    ..These findings suggest that the amino terminus of Pta1 has an inhibitory effect and that this effect can be neutralized through the interaction with Ssu72...
  8. ncbi The 73 kD subunit of the cleavage and polyadenylation specificity factor (CPSF) complex affects reproductive development in Arabidopsis
    Ruqiang Xu
    Department of Botany, Miami University, Oxford, OH 45056, USA
    Plant Mol Biol 61:799-815. 2006
    ..There are two CPSF73 like proteins (AtCPSF73-I and AtCPSF73-II) that share homology with the 73 kD subunit of the mammalian CPSF ..
  9. pmc Serotype IX, a Proposed New Streptococcus agalactiae Serotype
    Hans Christian Slotved
    Neisseria and Streptococcus Reference Laboratory, Department for Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark
    J Clin Microbiol 45:2929-36. 2007
    ..They all share the same, unique partial cps sequence. These results indicate that these eight isolates represent a new S. agalactiae serotype, which we propose should be designated serotype IX...
  10. pmc N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and alpha CaMKII mRNA polyadenylation at synapses
    Yi Shuian Huang
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    EMBO J 21:2139-48. 2002
    ..These data demonstrate a remarkable conservation of the regulatory machinery that controls signal-induced mRNA translation, and elucidates an axis connecting the NMDA receptor to localized protein synthesis at synapses...
  11. doi Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts
    Jong Heon Kim
    Research Institute, National Cancer Center, Goyang, Gyeonggi, South Korea
    Methods Enzymol 448:119-38. 2008
    ..In this chapter, we present detailed information for measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts...
  12. pmc The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae
    Alexander Zhelkovsky
    Department of Molecular Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
    RNA 12:435-45. 2006
    ..Our findings suggest that Syc1, by mimicking the essential Brr5 C-terminus, serves as a negative regulator of mRNA 3'-end formation...
  13. ncbi AtCPSF73-II gene encoding an Arabidopsis homolog of CPSF 73 kDa subunit is critical for early embryo development
    Ruqiang Xu
    Department of Botany, Miami University, Oxford, OH 45056, USA
    Gene 324:35-45. 2004
    ..Protein sequence analysis revealed a group of AtCPSF73-II homologs with unknown function in animals, but not in yeast, which suggested a potential important function of this group of genes in the development of multicellular organisms...
  14. pmc Formation of the 3' end of histone mRNA: getting closer to the end
    Zbigniew Dominski
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Gene 396:373-90. 2007
    ..The greatest challenge that lies ahead is to determine how all these factors interact with each other to form a catalytically competent processing complex capable of cleaving histone pre-mRNAs...
  15. pmc hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage
    Christopher T Richie
    Department of Molecular Genetics, M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Cell Biol 22:8635-47. 2002
    ..Five homologs of SNM1 have been identified in the mammalian genome and are termed SNM1, SNM1B, Artemis, ELAC2, and CPSF73. To explore the functional role of human Snm1 in response to DNA damage, we characterized the cellular ..
  16. pmc Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination
    Bernhard Dichtl
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 21:4125-35. 2002
    ..We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination...
  17. ncbi The 73 kDa subunit of the CPSF complex binds to the HIV-1 LTR promoter and functions as a negative regulatory factor that is inhibited by the HIV-1 Tat protein
    Laureano de La Vega
    Departamento de Biologia Celular, Fisiologia e Inmunologia, Universidad de Cordoba, Facultad de Medicina, Avda de Menendez Pidal s n, 14004, Cordoba, Spain
    J Mol Biol 372:317-30. 2007
    ..Our results clearly show a novel function for CPSF-73 and add another candidate protein for explaining the molecular mechanisms underlying HIV-1 latency...
  18. ncbi A history of poly A sequences: from formation to factors to function
    Mary Edmonds
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA
    Prog Nucleic Acid Res Mol Biol 71:285-389. 2002
    ..Thus, in spite of 40 years of study of poly A sequences, this growing multiplicity of uses and even mechanisms of formation seem destined to continue...
  19. ncbi [Is it reality that the endonuclease that cleaves pre-mRNA on polyadenylation has not been discovered?]
    M I Zarudnaia
    Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv
    Ukr Biokhim Zh 73:128-34. 2001
    ..The results of these works do not contradict the hypothesis that exactly CPSF-30 and its homologues are the actual nucleases that cleave pre-mRNA in the process of polyadenylation...
  20. pmc Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes
    Ilse Hofmann
    Division of Cell Biology, German Cancer Research Center, D 69120 Heidelberg, Germany
    Mol Biol Cell 13:1665-76. 2002
    ....
  21. doi Identification of virulence determinants in uropathogenic Proteus mirabilis using signature-tagged mutagenesis
    Stephanie D Himpsl
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Med Microbiol 57:1068-78. 2008
    ....
  22. ncbi HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin
    Hongyan Xing
    Department of Molecular and Cellular Biochemistry, Chandler Medical Center, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 279:10551-5. 2004
    ..Thus, HSF1 regulates HSP gene expression at not one but two different steps of the expression pathway, functioning both as a transcription factor and a polyadenylation stimulatory factor...
  23. pmc Unique features of plant cleavage and polyadenylation specificity factor revealed by proteomic studies
    Hongwei Zhao
    Department of Botany, Miami University, Oxford, Ohio 45056, USA
    Plant Physiol 151:1546-56. 2009
    ..Interestingly, these two unique plant CPSF components have been associated with embryo development and flowering time controls, both of which involve plant-specific biological processes...
  24. pmc The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis
    Monika Garas
    Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    RNA 14:2671-84. 2008
    ..These observations suggest that Ysh1p has multiple roles in RNA synthesis and processing...
  25. pmc Arabidopsis CLP1-SIMILAR PROTEIN3, an ortholog of human polyadenylation factor CLP1, functions in gametophyte, embryo, and postembryonic development
    Denghui Xing
    Botany Department, Miami University, Oxford, Ohio 45056, USA
    Plant Physiol 148:2059-69. 2008
    ..These observations indicate that Arabidopsis CLPS3 might be involved in the processing of pre-mRNAs encoded by a distinct subset of genes that are important in plant development...
  26. ncbi Cytoplasmic CstF-77 protein belongs to a masking complex with cytoplasmic polyadenylation element-binding protein in Xenopus oocytes
    Christel Rouget
    Centre de Recherches de Biochimie Macromoleculaire, CNRS, 1919 Route de Mende, 34293 Montpellier Cedex 05, France
    J Biol Chem 281:28687-98. 2006
    ..However, the kinetic of Mos mRNA polyadenylation is not modified. Furthermore, X77K represses mRNA translation in vitro. These results suggest that X77K could be involved in masking of mRNA prior to polyadenylation...
  27. pmc A serendipitous discovery that in situ proteolysis is essential for the crystallization of yeast CPSF-100 (Ydh1p)
    Corey R Mandel
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1041-5. 2006
    ..With the removal of this segment, yeast CPSF-100 (Ydh1p) has greatly reduced solubility and can be crystallized in the presence of a minute amount of precipitant...
  28. ncbi Opposing polymerase-deadenylase activities regulate cytoplasmic polyadenylation
    Jong Heon Kim
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell 24:173-83. 2006
    ..However, because PARN is more active than Gld2, the poly(A) tail is short. When oocytes mature, CPEB phosphorylation causes PARN to be expelled from the ribonucleoprotein complex, which allows Gld2 to elongate poly(A) by default...
  29. pmc Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor
    Uwe Kuhn
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    J Biol Chem 284:22803-14. 2009
    ..PABPN1 measures the length of the tail and is responsible for disrupting the CPSF-poly(A) polymerase interaction...
  30. pmc Capsular polysaccharide production in Enterococcus faecalis and contribution of CpsF to capsule serospecificity
    Lance R Thurlow
    Division of Biology, Kansas State University, 119 Ackert Hall, Manhattan, KS 66506, USA
    J Bacteriol 191:6203-10. 2009
    ..High-pH anion-exchange chromatography with pulsed amperometric detection analysis of serotype C and D capsules indicated that cpsF is responsible for glucosylation of serotype C capsular polysaccharide in E. faecalis...
  31. pmc Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease
    Kevin Ryan
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    RNA 10:565-73. 2004
    ..Taken together, the available data provide strong evidence that CPSF-73 is the 3' processing endonuclease...
  32. pmc Archaeal β-CASP ribonucleases of the aCPSF1 family are orthologs of the eukaryal CPSF-73 factor
    Duy Khanh Phung
    Centre National de la Recherche Scientifique, LMGM and Université de Toulouse, UPS, F 31062 Toulouse, France
    Nucleic Acids Res 41:1091-103. 2013
    ..Altogether, our results suggest that aCPSF1 performs an essential function and that an enzyme with similar activities was present in the last common ancestor of Archaea and Eukarya...
  33. ncbi Cleavage and polyadenylation specificity factor (CPSF)-derived peptides can induce HLA-A2-restricted and tumor-specific CTLs in the majority of gastrointestinal cancer patients
    Yoshiaki Maeda
    Department of Immunology, Kurume University School of Medicine, Kurume, Japan
    Int J Cancer 99:409-17. 2002
    ..Thus, these peptides might be appropriate molecules for use in the peptide-based specific immunotherapy of HLA-A2(+) patients with gastrointestinal cancers...
  34. pmc Phosphorylation of TFIIB links transcription initiation and termination
    Yuming Wang
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M139PT, UK
    Curr Biol 20:548-53. 2010
    ..Our results reveal that phosphorylation of TFIIB is a critical event in transcription that links the gene promoter and terminator and triggers initiation by RNA pol II...
  35. pmc The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA
    W Schul
    E C Slater Institute, BioCentrum, University of Amsterdam, The Netherlands
    EMBO J 15:2883-92. 1996
    ..These findings reveal a dynamic and transcription-dependent interaction between different subnuclear domains, and suggest a relationship between coiled bodies and specific transcripts...
  36. ncbi Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF
    C A Salinas
    Department of Zoology, University of British Columbia, Vancouver, Canada
    Mol Gen Genet 257:672-80. 1998
    ..Mutations in the cpsf gene did not alter the phenotype of homozygous su(f) mutations, suggesting that, for most genes, processing of 3' termini is not sensitive to small changes in cpsf and su(f) dosage...
  37. doi Genome-wide analysis of pre-mRNA 3' end processing reveals a decisive role of human cleavage factor I in the regulation of 3' UTR length
    Georges Martin
    Computational and Systems Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Cell Rep 1:753-63. 2012
    ....
  38. pmc snRNA 3' end formation requires heterodimeric association of integrator subunits
    Todd R Albrecht
    Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, Texas, USA
    Mol Cell Biol 32:1112-23. 2012
    ..11 (IntS9/11) are thought to contain the catalytic activity based on their high sequence similarity to CPSF100 and CPSF73, which have been shown to be components of both the poly(A)(+) and histone pre-mRNA cleavage complex...
  39. pmc The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA
    Rakesh S Laishram
    Department of Pharmacology, University of Wisconsin, Medical Science Center, Madison, WI 53706, USA
    EMBO J 29:4132-45. 2010
    ..In vitro and in vivo Star-PAP was required for the stable association of CPSF complex to pre-mRNA and then CPSF 73 specifically cleaved the mRNA at the 3'-cleavage site...
  40. pmc A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor
    P J Preker
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    EMBO J 16:4727-37. 1997
    ..PF I also appears to be functionally related to CPSF, as it polyadenylates a substrate RNA more efficiently than Pap1p alone. Possibly, the observed interaction of the complex with RNA tethers Pap1p to its substrate...
  41. pmc Assembly of a processive messenger RNA polyadenylation complex
    S Bienroth
    Abteilung Zellbiologie, Universitat Basel, Switzerland
    EMBO J 12:585-94. 1993
    ..Only the complex formed from all three proteins is competent for the processive synthesis of a full-length poly(A) tail...
  42. pmc A complex containing the CPSF73 endonuclease and other polyadenylation factors associates with U7 snRNP and is recruited to histone pre-mRNA for 3'-end processing
    Xiao cui Yang
    Department of Biochemistry and Biophysics, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Mol Cell Biol 33:28-37. 2013
    ..The cleavage reaction is catalyzed by CPSF73 and depends on the U7 snRNP and its integral component, Lsm11...
  43. pmc Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor
    A Jenny
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Nucleic Acids Res 23:2629-35. 1995
    ..The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs. It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb)...
  44. ncbi The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide
    E Almiron-Roig
    Institute of Food Research, Norwich Research Park, Colney Lane, NR4 7UA Norwich, UK
    Microbiology 146:2793-802. 2000
    ..Analysis of the expression of the cpsE gene in Escherichia coli demonstrated that it encodes a glucose-1-phosphate transferase; the enzyme which catalyses the first step in EPS biosynthesis in S. thermophilus NCFB 2393...
  45. doi Few Smad proteins and many Smad-interacting proteins yield multiple functions and action modes in TGFβ/BMP signaling in vivo
    Andrea Conidi
    Laboratory of Molecular Biology Celgen of Center for Human Genetics, University of Leuven, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Cytokine Growth Factor Rev 22:287-300. 2011
    ..e. the CPSF subunit Smicl, Ttrap (Tdp2) and Sip1 (Zeb2, Zfhx1b) from our own research carried out in three different vertebrate models...
  46. ncbi A candidate prostate cancer susceptibility gene at chromosome 17p
    S V Tavtigian
    Myriad Genetics, Inc, Salt Lake City, Utah, USA
    Nat Genet 27:172-80. 2001
    ..amino acid sequence similarity to two better understood protein families, namely the PSO2 (SNM1) DNA interstrand crosslink repair proteins and the 73-kD subunit of mRNA 3' end cleavage and polyadenylation specificity factor (CPSF73).
  47. ncbi [Development of a yeast two-hybrid screen for selection of A/H1N1 influenza NS1 non-structural protein and human CPSF30 protein interaction inhibitors]
    Jian qiang Kong
    Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC and Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Health of PRC, Beijing 100050, China
    Yao Xue Xue Bao 45:388-94. 2010
    ..The four of more than thirty identified Chinese medicines, including 'Shuanghuanglian oral liquid', showed the strong inhibition of the NS1-CPSF30 interaction...
  48. doi Microarray analysis revealed that immunity-associated genes are primarily regulated by roxarsone in promoting broiler chicken (Gallus gallus domesticus) growth
    C L Li
    Department of Animal Science and Technology, China Agricultural University, Beijing 100094, China
    Poult Sci 91:3184-90. 2012
    ..factor X-associated ankyrin-containing protein (RFXANK), the cleavage and polyadenylation-specific factor 3 (CPSF3), and the FK506 binding protein 9 (FKBP9), have consistently up or downregulated (fold change ≥1.5 or ≤0...
  49. pmc HPV-16 E2 contributes to induction of HPV-16 late gene expression by inhibiting early polyadenylation
    Cecilia Johansson
    Section of Medical Microbiology, Department of Laboratory Medicine, Lund University, Lund, Sweden
    EMBO J 31:3212-27. 2012
    ..We speculate that the accumulation of high levels of E2 during the viral life cycle, not only turns off the expression of the pro-mitotic viral E6 and E7 genes, but also induces the expression of the late HPV genes L1 and L2...
  50. doi The ubiquitin hydrolase USP22 contributes to 3'-end processing of JAK-STAT-inducible genes
    Edmond Chipumuro
    Department of Biology, The University of Virginia, Charlottesville, VA 22903, USA
    FASEB J 26:842-54. 2012
    ..Furthermore, the polyadenylation factor CPSF73 is not effectively recruited, and serine 2 phosphorylation (Ser2P) of the C-terminal domain of RNA polymerase II ..
  51. pmc Genes involved in pre-mRNA 3'-end formation and transcription termination revealed by a lin-15 operon Muv suppressor screen
    Mingxue Cui
    Department of Molecular, Cellular, and Developmental Biology, and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309
    Proc Natl Acad Sci U S A 105:16665-70. 2008
    ..Furthermore, our results implicate a serine/arginine-rich (SR) protein, SRp20, in events following 3'-end cleavage, leading to termination of transcription...
  52. pmc CSR1 induces cell death through inactivation of CPSF3
    Z H Zhu
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Oncogene 28:41-51. 2009
    ..cDNA library using a yeast two-hybrid system and found that the cleavage and polyadenylation-specific factor 3 (CPSF3), an essential component for converting heteronuclear RNA to mRNA, binds with high affinity to the CSR1 C terminus...
  53. pmc Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation
    Nikolay G Kolev
    Howard Hughes Medical Institute, Yale University, 295 Congress Avenue, New Haven, Connecticut 06519, USA
    EMBO Rep 9:1013-8. 2008
    ..This indicates that CPSF73 and CPSF100 act together in the process of maturation of eukaryotic pre-messenger RNAs, similar to other members ..
  54. pmc Use of phenotypic and molecular serotype identification methods to characterize previously nonserotypeable group B streptococci
    Fanrong Kong
    Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Darcy Road, Westmead, New South Wales 2145, Australia
    J Clin Microbiol 46:2745-50. 2008
    ..001), but further investigation is needed to determine the genetic basis for most nonserotypeable GBS isolates...
  55. pmc Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing
    Xiao cui Yang
    Program in Molecular Biology and Biotechnology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 29:31-42. 2009
    ..RNA interference experiments with HeLa cells indicate that degradation of the DCP does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP both in vitro and in vivo...
  56. doi An active role for splicing in 3'-end formation
    Harold G Martinson
    Department of Chemistry and Biochemistry, Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA, USA
    Wiley Interdiscip Rev RNA 2:459-70. 2011
    ..However, the U1 snRNP can also bind elsewhere in the transcript, apart from splice sites, to regulate CP by direct interaction with the CP factors...
  57. ncbi A procedure for calculation of monitor units for passively scattered proton radiotherapy beams
    Narayan Sahoo
    Department of Radiation Physics, UT MD Anderson Cancer Center, 1515 Holcombe Boulevard, Box 1150, Houston, Texas 77030, USA
    Med Phys 35:5088-97. 2008
    ..The authors conclude that an intuitive formula similar to the one used for monitor unit calculation of therapeutic photon beams can be used to compute the monitor units of passively scattered proton therapy beams...
  58. ncbi Alternative polyadenylation of MeCP2: Influence of cis-acting elements and trans-acting factors
    Catherine M Newnham
    Department of Physiology and Experimental Medicine, University of Toronto, Toronto, ON, Canada
    RNA Biol 7:361-72. 2010
    ..In sum, the mechanism(s) of regulated alternative 3'UTR usage of MeCP2 are complex, and insight into these mechanisms will aid our understanding of the factors that influence MeCP2 expression...
  59. pmc Analysis of spliceosomal proteins in Trypanosomatids reveals novel functions in mRNA processing
    Itai Dov Tkacz
    Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar Ilan University, Ramat Gan 52900, Israel
    J Biol Chem 285:27982-99. 2010
    ..nuclear ribonucleoprotein (snRNP) proteins, additional co-purified proteins, including the polyadenylation factor CPSF73. Defects observed in cells silenced for U1 snRNP proteins suggest that the U1 snRNP functions exclusively in cis-..
  60. pmc FLASH, a proapoptotic protein involved in activation of caspase-8, is essential for 3' end processing of histone pre-mRNAs
    Xiao cui Yang
    Department of Biochemistry and Biophysics and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 36:267-78. 2009
    ..These results demonstrate that FLASH is an essential factor required for 3' end maturation of histone mRNAs in both vertebrates and invertebrates and suggest a potential link between this process and apoptosis...
  61. doi Crystal structure of a dimeric archaeal cleavage and polyadenylation specificity factor
    Bijàn Mir-Montazeri
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    J Struct Biol 173:191-5. 2011
    ..It reveals a dimerization mode of the MβL domain that has not been observed before and suggests that RNA is bound across the dimer interface, recognized by the KH domains of one monomer, and cleaved at the active site of the other...
  62. pmc The telomeric protein SNM1B/Apollo is required for normal cell proliferation and embryonic development
    Shamima Akhter
    Department of Genetics, The UT MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Aging Cell 9:1047-56. 2010
    ..Although Snm1B/Apollo-deficient MEFs exhibited high levels of apoptosis, abrogation of p53-dependent programmed cell death did not rescue the multi-organ developmental failure in the mice...
  63. pmc Smicl is required for phosphorylation of RNA polymerase II and affects 3'-end processing of RNA at the midblastula transition in Xenopus
    Clara Collart
    Wellcome Trust CR UK Gurdon Institute and Department of Zoology, University of Cambridge, Cambridge, UK
    Development 136:3451-61. 2009
    ..Our work links the onset of zygotic gene expression in the Xenopus embryo with the translocation of Smicl from cytoplasm to nucleus, the phosphorylation of Rpb1 and the 3'-end processing of newly transcribed mRNAs...
  64. pmc Inositol 1,4,5-triphosphate receptor-binding protein released with inositol 1,4,5-triphosphate (IRBIT) associates with components of the mRNA 3' processing machinery in a phosphorylation-dependent manner and inhibits polyadenylation
    Hélène Kiefer
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 284:10694-705. 2009
    ....
  65. doi 2-D DIGE identification of differentially expressed heterogeneous nuclear ribonucleoproteins and transcription factors during neural differentiation of human embryonic stem cells
    Miguel Barthelery
    Pennsylvania State University College of Medicine, Department of Pharmacology, Hummelstown, PA, USA
    Proteomics Clin Appl 3:505-14. 2009
    ..By contrast, Cpsf-6 virtually disappears with differentiation with an 11-fold drop in NSC, highlighting this protein as a novel marker for undifferentiated ESC...
  66. pmc FLASH is required for the endonucleolytic cleavage of histone pre-mRNAs but is dispensable for the 5' exonucleolytic degradation of the downstream cleavage product
    Xiao cui Yang
    Department of Biochemistry and Biophysics, Program in Molecular Biology and Biotechnology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 31:1492-502. 2011
    ..These results suggest that CPSF-73, the catalytic component in both reactions, can be recruited to histone pre-mRNA largely in a manner independent of FLASH, possibly by a separate domain in Lsm11...
  67. doi New regulatory mechanisms for the intracellular localization and trafficking of influenza A virus NS1 protein revealed by comparative analysis of A/PR/8/34 and A/Sydney/5/97
    Han Han
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences Wuhan 430071, PR China
    J Gen Virol 91:2907-17. 2010
    ..These findings provide new insights into the mechanism of intracellular localization and trafficking of influenza A virus NS1 protein, which is important for understanding its function...
  68. pmc Distinctive interactions of the Arabidopsis homolog of the 30 kD subunit of the cleavage and polyadenylation specificity factor (AtCPSF30) with other polyadenylation factor subunits
    Suryadevara Rao
    Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546 0312, USA
    BMC Cell Biol 10:51. 2009
    ..In order to further explore the functions of AtCPSF30, the subcellular distribution of the protein was examined by over-expressing fusion proteins containing fluorescent reporters linked to different CPSF subunits...
  69. pmc A role for Chk1 in blocking transcriptional elongation of p21 RNA during the S-phase checkpoint
    Rachel Beckerman
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 23:1364-77. 2009
    ..These findings demonstrate for the first time a link between the replication checkpoint mediated by ATR/Chk1 and the transcription elongation/3' processing machinery...
  70. doi Post-transcriptional and post-translational regulation during mouse oocyte maturation
    Min Kook Kang
    School of Biological Sciences, Inje University, Gimhae, Korea
    BMB Rep 44:147-57. 2011
    ..RNA interfering is a new regulatory mechanism of the amount of mRNA in the mouse oocyte. This review summarizes information about post-transcriptional and post-translational regulation during mouse oocyte meiotic maturation...
  71. pmc The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors
    Orit Rozenblatt-Rosen
    Department of Medical Oncology, Dana Farber Cancer Institute, and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:755-60. 2009
    ..Our results suggest that Cdc73 facilitates association of 3' mRNA processing factors with actively-transcribed chromatin and support the importance of links between tumor suppression and mRNA maturation...
  72. pmc Translational control by cytoplasmic polyadenylation in Xenopus oocytes
    Helois E Radford
    School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK
    Biochim Biophys Acta 1779:217-29. 2008
    ..Finally we discuss some of the remaining questions regarding the mechanisms of translational regulation by cytoplasmic polyadenylation and give our view on where our knowledge is likely to be expanded in the near future...
  73. pmc CKIalpha is associated with and phosphorylates star-PAP and is also required for expression of select star-PAP target messenger RNAs
    Michael L Gonzales
    Molecular and Cellular Pharmacology Training Program, Department of Pharmacology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 283:12665-73. 2008
    ..The Star-PAP complex therefore represents a location where multiple signaling pathways converge to regulate the expression of specific mRNAs...
  74. pmc Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit
    A Jenny
    Department of Cell Biology, University of Basel, Switzerland
    Mol Cell Biol 14:8183-90. 1994
    ..Immunofluorescent detection of CPSF in HeLa cells localized it in the nucleoplasm, excluding cytoplasm and nucleolar structures...
  75. pmc Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family
    Isabelle Callebaut
    Systèmes moléculaires et Biologie structurale, LMCP, CNRS UMR 7590, Universités Paris 6 et Paris 7, case 115, 4 place Jussieu, F 75252 Paris Cedex 05, France
    Nucleic Acids Res 30:3592-601. 2002
    ....
  76. ncbi CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes
    R Hodgman
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 128:2815-22. 2001
    ..These data indicate that cytoplasmic polyadenylation in mouse oocytes is mediated by IAK1/Eg2-catalyzed phosphorylation of CPEB...
  77. ncbi Overexpression of the CstF-64 and CPSF-160 polyadenylation protein messenger RNAs in mouse male germ cells
    B Dass
    Department of Cell Biology and Biochemistry and Southwest Cancer Center at University Medical Center, Texas Tech University Health Sciences Center, Lubbock, Texas 79430
    Biol Reprod 64:1722-9. 2001
    ..Proc Natl Acad Sci U S A 1999; 96:6763-6768), which suggests that overexpression of mRNA transcription and processing factors plays an essential role in postmeiotic germ cell mRNA metabolism...
  78. ncbi Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex
    R Mendez
    Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Cell 6:1253-9. 2000
    ..These results demonstrate that the molecular function of Eg2-phosphorylated CPEB is to recruit CPSF into an active cytoplasmic polyadenylation complex...
  79. pmc Distinct roles of two Yth1p domains in 3'-end cleavage and polyadenylation of yeast pre-mRNAs
    S M Barabino
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:3778-87. 2000
    ..Finally, we find that Yth1p binds to CYC1 pre-mRNA in the vicinity of the cleavage site. Our results indicate that Yth1p is important for the integrity of CPF and participates in the recognition of the cleavage site...
  80. pmc Complex protein interactions within the human polyadenylation machinery identify a novel component
    Y Takagaki
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Mol Cell Biol 20:1515-25. 2000
    ..These and other data suggest that symplekin may function in assembly of the polyadenylation machinery...
  81. pmc The cleavage and polyadenylation specificity factor in Xenopus laevis oocytes is a cytoplasmic factor involved in regulated polyadenylation
    K S Dickson
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell Biol 19:5707-17. 1999
    ..laevis form of the 30-kDa subunit of CPSF is involved in this reaction. Together, these results indicate that a distinct, cytoplasmic form of CPSF is an integral component of the cytoplasmic polyadenylation machinery...
  82. pmc Influenza A virus NS1 protein targets poly(A)-binding protein II of the cellular 3'-end processing machinery
    Z Chen
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08855, USA
    EMBO J 18:2273-83. 1999
    ..In vitro assays suggest that the 30 kDa CPSF and PABII proteins bind to non-overlapping regions of the NS1A protein effector domain and indicate that these two 3' processing proteins also directly bind to each other...
  83. ncbi Influenza virus NS1 protein interacts with the cellular 30 kDa subunit of CPSF and inhibits 3'end formation of cellular pre-mRNAs
    M E Nemeroff
    Department of Molecular Biology and Biochemistry Rutgers University Piscataway, New Jersey 08854, USA
    Mol Cell 1:991-1000. 1998
    ..Via this novel regulation of pre-mRNA 3' end processing, the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs...
  84. pmc Drosophila clipper/CPSF 30K is a post-transcriptionally regulated nuclear protein that binds RNA containing GC clusters
    C Bai
    Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA
    Nucleic Acids Res 26:1597-604. 1998
    ..These results support the existence of a distinct mechanism controlling eukaryotic gene expression through the regulated processing of pre-mRNAs in the nucleus...
  85. ncbi Transcription factor TFIID recruits factor CPSF for formation of 3' end of mRNA
    J C Dantonel
    Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS INSERM ULP, College de France, Strasbourg
    Nature 389:399-402. 1997
    ..Our observations have thus revealed a link between transcription initiation and elongation by RNA polymerase II and processing of the 3' end of mRNA...
  86. ncbi Dependence of yeast pre-mRNA 3'-end processing on CFT1: a sequence homolog of the mammalian AAUAAA binding factor
    G Stumpf
    Institut fur Biochemie, Genzentrum, der Ludwig Maximilians Universität München Feodor Lynen Str 25, D 81377 Munchen, Germany
    Science 274:1517-20. 1996
    ..Polyadenylation required the further addition of poly(A) polymerase and polyadenylation factor I, suggesting a close but not necessarily direct association of these two factors with the CFT1 protein...
  87. ncbi Essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF)
    G Chanfreau
    Department of Biochemistry and Biophysics, UCSF School of Medicine, San Francisco, CA 94143 0448
    Science 274:1511-4. 1996
    ..These results demonstrate a structural and functional conservation of the yeast and mammalian 3'-end processing machineries despite a lack of conservation of the cis sequences...
  88. ncbi Characterization of cpsF and its product CMP-N-acetylneuraminic acid synthetase, a group B streptococcal enzyme that can function in K1 capsular polysaccharide biosynthesis in Escherichia coli
    R F Haft
    Channing Laboratory, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Mol Microbiol 19:555-63. 1996
    ..We concluded that cpsF encodes CMP-Neu5Ac synthetase in type III GBS, and that the GBS enzyme can function in the capsule-synthesis of a heterologous bacterial species...
  89. ncbi Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors
    U Rüegsegger
    Department of Cell Biology, Biozentrum, Universitat Basel, Switzerland
    J Biol Chem 271:6107-13. 1996
    ..Furthermore, the CstF-CPSF-RNA as well as the CstF-CPSF-PAP-RNA complex are supershifted and stabilized upon the addition of CF Im...
  90. pmc Multiple forms of poly(A) polymerases in human cells
    A C Thuresson
    Department of Medical Genetics, Uppsala University, Sweden
    Proc Natl Acad Sci U S A 91:979-83. 1994
    ..Interestingly, tentative phosphorylation sites have been identified in this region, suggesting that phosphorylation/dephosphorylation may regulate the interaction between the two polyadenylylation factors PAP and CPSF...
  91. pmc Functional dissection of the zinc finger and flanking domains of the Yth1 cleavage/polyadenylation factor
    Yoko Tacahashi
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Sackler Graduate School of Biomedical Sciences, Boston, MA 02111, USA
    Nucleic Acids Res 31:1744-52. 2003
    ..Our results suggest a role for Yth1 not only in the execution of cleavage and poly(A) addition, but also in the transition from one step to the other...
  92. pmc Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase
    Isabelle Kaufmann
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    EMBO J 23:616-26. 2004
    ..These results show that hFip1 significantly contributes to CPSF-mediated stimulation of PAP activity...
  93. ncbi Structure of primate and rodent orthologs of the prostate cancer susceptibility gene ELAC2
    Martine Dumont
    Canada Research Chair in Oncogenetics and Cancer Genomics Laboratory, CHUL Research Center and Laval University, 2705 Laurier Boulevard, Sainte Foy, Quebec City, Canada G1V 4G2
    Biochim Biophys Acta 1679:230-47. 2004
    ..cross-link repair proteins, and the 73-kDa subunit of mRNA 3' end cleavage and polyadenylation specificity factor (CPSF73) as well as Artemis proteins, thus suggesting a potential interaction of ELAC2 gene product with nucleic acids and ..
  94. pmc Calmodulin interacts with and regulates the RNA-binding activity of an Arabidopsis polyadenylation factor subunit
    Kimberly J Delaney
    Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky 40546 0312, USA
    Plant Physiol 140:1507-21. 2006
    ....
  95. pmc Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease
    Corey R Mandel
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Nature 444:953-6. 2006
    ..Our studies provide the first direct experimental evidence that CPSF-73 is the pre-mRNA 3'-end-processing endonuclease...
  96. ncbi The VP16 activation domain establishes an active mediator lacking CDK8 in vivo
    Thomas Uhlmann
    Gene Expression, National Research Center for Environment and Health, Marchionini Strasse 25, D 81377 Munich, Germany
    J Biol Chem 282:2163-73. 2007
    ..We provide in vitro and in vivo evidence that VP16 activates transcription through a specific MED25-associated Mediator, which is deficient in CDK8...
  97. pmc Multiple anti-interferon actions of the influenza A virus NS1 protein
    Georg Kochs
    Department of Virology, University of Freiburg, D 79008 Freiburg, Germany
    J Virol 81:7011-21. 2007
    ..Our results demonstrate the capacity of FLUAV NS1 to suppress the antiviral host defense at multiple levels and the existence of strain-specific differences that may modulate virus pathogenicity...
  98. ncbi Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolism
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Crit Rev Biochem Mol Biol 42:67-93. 2007
    ..This article reviews the cellular roles of nucleases of the metallo-beta-lactamase family and the recent advances in studying these proteins...
  99. pmc Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex
    Vasupradha Vethantham
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell Biol 27:8848-58. 2007
    ....
  100. ncbi Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells
    Tomoko Nakanishi
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Biochem Biophys Res Commun 364:14-9. 2007
    ..Thus mGLD-2 is dispensable for full-term development and oogenesis. Our results also indicate that there is another source of cytoplasmic poly(A) polymerase in mouse...
  101. doi Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex
    Catherine Papin
    Centre de Recherche en Biochimie Macromoleculaire, Universite Montpellier II, France
    FEBS J 275:490-503. 2008
    ..Unexpectedly, XGld2 and CPEB antibody injections also led to an acceleration of meiotic maturation, suggesting that XGld2 is part of a masking complex with CPEB and is associated with repressed mRNAs in oocytes...

Research Grants45

  1. Histone pre-mRNA processing in Drosophila Melanogaster
    Mindy Steiniger; Fiscal Year: 2007
    ..Therefore, a better understanding of histone pre-mRNA processing will lead to a better understanding of cancer. ..
  2. The Role of a New Cleavage and Polyadenylation Specificity Factor (CPSF73-II)
    Qingshun Li; Fiscal Year: 2007
    ..The goal of this proposal is to understand how one of these paralogues (CPSF73-II) works to regulate 3'- end formation of mRNAs via a combination of biochemical and molecular genetic means ..
  3. Structural biology of pre-mRNA 3'-end processing
    Liang Tong; Fiscal Year: 2010
    ..These initial results set the stage for further biochemical, biophysical, and structural studies on these proteins with crucial roles in pre-mRNA 3'-end processing. ..
  4. Structural biology of pre-mRNA 3'-end processing
    Liang Tong; Fiscal Year: 2009
    ..These initial results set the stage for further biochemical, biophysical, and structural studies on these proteins with crucial roles in pre-mRNA 3'-end processing. ..
  5. Structural biology of pre-mRNA 3'-end processing
    Liang Tong; Fiscal Year: 2007
    ..These initial results set the stage for further biochemical, biophysical, and structural studies on these proteins with crucial roles in pre-mRNA 3'-end processing. ..
  6. POLYADENYLATION OF AN ESTROGEN REGULATED MESSENGER RNA
    Daniel Schoenberg; Fiscal Year: 2000
    ....
  7. POLYADENYLATION AND TRANSLATIONAL CONTROL
    Joel Richter; Fiscal Year: 2009
    ..Finally, an upstream event that is necessary for CPEB-mediated polyadenylation is the translational activation of RINGO/Spy mRNA, which encodes an atypical cyclin B1-like protein. Pumilio is the ..
  8. POLYADENYLATION AND TRANSLATIONAL CONTROL
    Joel Richter; Fiscal Year: 2007
    ..Therefore, mechanistic studies of polyadenylation-induced translation are likely to have important implications for human health. ..
  9. POLYADENYLATION AND TRANSLATIONAL CONTROL
    Joel Richter; Fiscal Year: 2009
    ..In particular, we will examine RNA processing and translational control. Because of the fundamental nature of this work, it has important implications for fertility, neurodegeneration, and cancer. ..
  10. POLYADENYLATION AND TRANSLATIONAL CONTROL
    Joel D Richter; Fiscal Year: 2010
    ..In particular, we will examine RNA processing and translational control. Because of the fundamental nature of this work, it has important implications for fertility, neurodegeneration, and cancer. ..
  11. Small RNP Mediators of Gene Expression
    Joan A Steitz; Fiscal Year: 2010
    ..These studies will illuminate a variety of human disease processes that can be traced to perturbations in small RNP biogenesis, functions and associations. ..
  12. Small RNP Mediators of Gene Expression
    JOAN STEITZ; Fiscal Year: 2007
    ..These studies will illuminate a variety of human disease processes that can be traced to perturbations in small RNP biogenesis, functions and associations. ..
  13. Small RNP Mediators of Gene Expression
    JOAN STEITZ; Fiscal Year: 2009
    ..These studies will illuminate a variety of human disease processes that can be traced to perturbations in small RNP biogenesis, functions and associations. ..
  14. mRNA synthesis in animal cells - 3' end formation
    James Manley; Fiscal Year: 2009
    ..Finally, a reconstituted coupled transcription-3' processing system will be developed and, using chromatinized templates, the function of PAF in linking transcription, chromatin modification and 3' processing analyzed. ..
  15. mRNA synthesis in animal cells - 3' end formation
    James Manley; Fiscal Year: 2009
    ..Finally, a reconstituted coupled transcription-3' processing system will be developed and, using chromatinized templates, the function of PAF in linking transcription, chromatin modification and 3' processing analyzed. ..
  16. mRNA synthesis in animal cells - 3' end formation
    James Manley; Fiscal Year: 2007
    ..Finally, a reconstituted coupled transcription-3' processing system will be developed and, using chromatinized templates, the function of PAF in linking transcription, chromatin modification and 3' processing analyzed. ..
  17. mRNA synthesis in animal cells - 3' end formation
    James Manley; Fiscal Year: 2007
    ..Finally, a reconstituted coupled transcription-3' processing system will be developed and, using chromatinized templates, the function of PAF in linking transcription, chromatin modification and 3' processing analyzed. ..
  18. RNAi, Histone Modification and the DDB1/CPSF-like Complex Rik1
    Robert Martienssen; Fiscal Year: 2007
    ..Transposable elements and other repeats are common to all of these genomes, and may hold the key to epigenetic mechanisms of gene regulation and chromosome function. ..
  19. RNAi, Histone Modification and the DDB1/CPSF-like Complex Rik1
    Robert A Martienssen; Fiscal Year: 2010
    ..Transposable elements and other repeats are common to all of these genomes, and may hold the key to epigenetic mechanisms of gene regulation and chromosome function. ..
  20. Elucidation of Integrator's Function
    Ramin Shiekhattar; Fiscal Year: 2010
    ..Aberrant regulation of small nuclear RNA processing could result in defective messenger RNA processing through deregulation of splicesome and may lead to cellular transformation and oncogenesis. ..
  21. MRNA SYTHESIS IN ANIMAL CELLS--3END FORMATION
    James Manley; Fiscal Year: 2001
    ....
  22. mRNA synthesis in animal cells - 3' end formation
    James L Manley; Fiscal Year: 2010
    ..Recent studies have revealed that changes in polyadenylation occur during development and disease, and our studies will provide a mechanistic understanding for these changes. ..
  23. RENOVATION SUPPLEMENT FOR COBRE
    Francis Millett; Fiscal Year: 2002
    ..The room would be converted into four new research laboratories needed for the Center in the following areas: 1) protein preparation, protein folding, drug design, and membrane protein Interaction. ..
  24. MECHANISM OF POLY(A)-DEPENDENT TRANSCRIPTION TERMINATION
    HAROLD MARTINSON; Fiscal Year: 2004
    ..Then the possibility is considered of using immunodepletion to identify polyA-dependent termination factors from among proteins that have already been described. ..
  25. New Coding Vectors for microRNA and siRNA
    Kevin Ryan; Fiscal Year: 2006
    ..abstract_text> ..