CPSF1

Summary

Gene Symbol: CPSF1
Description: cleavage and polyadenylation specific factor 1
Alias: CPSF160, HSU37012, P/cl.18, cleavage and polyadenylation specificity factor subunit 1, CPSF 160 kDa subunit, cleavage and polyadenylation specific factor 1, 160kDa, cleavage and polyadenylation specificity factor 160 kDa subunit, polyadenylation specificity factor
Species: human
Products:     CPSF1

Top Publications

  1. Murthy K, Manley J. The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation. Genes Dev. 1995;9:2672-83 pubmed
    Cleavage-polyadenylation specificity factor (CPSF) is a multisubunit protein that plays a central role in 3' processing of mammalian pre-mRNAs...
  2. Pettinati I, Grzechnik P, Ribeiro de Almeida C, Brem J, McDonough M, Dhir S, et al. Biosynthesis of histone messenger RNA employs a specific 3' end endonuclease. elife. 2018;7: pubmed publisher
    ..A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole endonuclease responsible for 3' end processing of both mRNA ..
  3. Martens B, Amman F, Manoharadas S, Zeichen L, Orell A, Albers S, et al. Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2. PLoS ONE. 2013;8:e76569 pubmed publisher
    ..Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins...
  4. Thore S, Fribourg S. Structural insights into the 3'-end mRNA maturation machinery: snapshot on Polyadenylation signal recognition. Biochimie. 2019;: pubmed publisher
    ..recent findings regarding the atomic description of one the major players, the Cleavage and Polyadenylation Specificity Factor complex (CPSF in human, CPF in yeast)...
  5. Nilubol N, Boufraqech M, Zhang L, Kebebew E. Loss of CPSF2 expression is associated with increased thyroid cancer cellular invasion and cancer stem cell population, and more aggressive disease. J Clin Endocrinol Metab. 2014;99:E1173-82 pubmed publisher
    ..Experiemental Design: We selected the cleavage and polyadenylation specificity factor subunit 2 (CPSF2) gene from the top 5 significantly dysregulated genes associated with PTC-..
  6. Moreno Morcillo M, Minvielle Sebastia L, Mackereth C, Fribourg S. Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure. RNA. 2011;17:412-8 pubmed publisher
    ..CstF recognizes U and G/U-rich cis-acting RNA sequence elements and helps stabilize the Cleavage and Polyadenylation Specificity Factor (CPSF) at the polyadenylation site as required for productive RNA cleavage...
  7. Dubois J, Traversier A, Julien T, Padey B, Lina B, Bourdon J, et al. The Nonstructural NS1 Protein of Influenza Viruses Modulates TP53 Splicing through Host Factor CPSF4. J Virol. 2019;93: pubmed publisher
    ..Our results demonstrate that CPSF4 (cellular protein cleavage and polyadenylation specificity factor 4) independently and the interaction between NS1 and CPSF4 modulate the alternative splicing of ..
  8. Zhang L, Wang J, Muñoz Moreno R, Kim M, Sakthivel R, Mo W, et al. Influenza Virus NS1 Protein-RNA Interactome Reveals Intron Targeting. J Virol. 2018;92: pubmed publisher
    ..a functional RNA binding domain and is independent of the NS1 interaction with the cleavage and polyadenylation specificity factor CPSF30...
  9. Luo J, Cheng L, Guan X, Li D, Yu L, Du L. [LC-MS/MS Analysis on the Components of Excretory-secretory Protein of Trichinella spiralis Muscle Larvae]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2016;34:53-7 pubmed
    ..Six anti-tumor relevant proteins were revealed, which were tropomyosin, histone H2A, cleavage and polyadenylation specificity factor subunit 2, serine proteinase inhibitor Kazal-type 4, Armadillo segment polarity protein and ..

More Information

Publications72

  1. Kleinpeter A, Jureka A, Falahat S, Green T, Petit C. Structural analyses reveal the mechanism of inhibition of influenza virus NS1 by two antiviral compounds. J Biol Chem. 2018;293:14659-14668 pubmed publisher
    ..in the hydrophobic pocket known to facilitate binding to the 30-kDa subunit of the cleavage and polyadenylation specificity factor (CPSF30), suggesting that the two compounds likely attenuate influenza replication by inhibiting the ..
  2. Misra A, Ou J, Zhu L, Green M. Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors. Mol Cell. 2015;58:819-31 pubmed publisher
    ..Unexpectedly, we find that two mRNA 3' end formation factors, cleavage and polyadenylation specificity factor (CPSF) and SYMPK, are RBFOX2 cofactors for both inclusion and exclusion of internal exons...
  3. Romeo V, Griesbach E, Schümperli D. CstF64: cell cycle regulation and functional role in 3' end processing of replication-dependent histone mRNAs. Mol Cell Biol. 2014;34:4272-84 pubmed publisher
    ..the U7 snRNP, but cleavage additionally requires a heat-labile factor (HLF), composed of cleavage/polyadenylation specificity factor, symplekin, and cleavage stimulation factor 64 (CstF64)...
  4. Zhao H, Ye X, Li Q. Characterization of plant polyadenylation complexes by using tandem affinity purification. Methods Mol Biol. 2015;1255:69-78 pubmed publisher
    ..Based on homology analyses, Arabidopsis cleavage and polyadenylation specificity factor (AtCPSF) complex should play a critical role in pre-mRNA 3' end processing...
  5. Ning J, Zhong Z, Fischer D, Harris G, Watkins S, Ambrose Z, et al. Truncated CPSF6 Forms Higher-Order Complexes That Bind and Disrupt HIV-1 Capsid. J Virol. 2018;92: pubmed publisher
    Cleavage and polyadenylation specificity factor 6 (CPSF6) is a human protein that binds HIV-1 capsid and mediates nuclear transport and integration targeting of HIV-1 preintegration complexes...
  6. Salazar K, Joffe N, Dinguirard N, Houde P, Castillo M. Transcriptome analysis of the white body of the squid Euprymna tasmanica with emphasis on immune and hematopoietic gene discovery. PLoS ONE. 2015;10:e0119949 pubmed publisher
    ..Several hematopoiesis genes including CPSF1, GATA 2, TFIID, and FGFR2 were found to be expressed in the white body...
  7. Schönemann L, Kühn U, Martin G, Schäfer P, Gruber A, Keller W, et al. Reconstitution of CPSF active in polyadenylation: recognition of the polyadenylation signal by WDR33. Genes Dev. 2014;28:2381-93 pubmed publisher
    Cleavage and polyadenylation specificity factor (CPSF) is the central component of the 3' processing machinery for polyadenylated mRNAs in metazoans: CPSF recognizes the polyadenylation signal AAUAAA, providing sequence specificity in ..
  8. Wall R, Rico E, Lukac I, Zuccotto F, Elg S, Gilbert I, et al. Clinical and veterinary trypanocidal benzoxaboroles target CPSF3. Proc Natl Acad Sci U S A. 2018;115:9616-9621 pubmed publisher
    ..screening approach was used to identify the target of the benzoxaboroles, Cleavage and Polyadenylation Specificity Factor 3 (CPSF3, Tb927.4.1340)...
  9. Zhang B, Liu Y, Liu D, Yang L. Targeting cleavage and polyadenylation specific factor 1 via shRNA inhibits cell proliferation in human ovarian cancer. J Biosci. 2017;42:417-425 pubmed
    Cleavage and polyadenylation specificity factor 1 (CPSF1), a member of CPSF complex, has been reported to play a key role in pre-mRNA 3'-end formation, but its possible role in ovarian cancer remains unclear...
  10. O Reilly D, Kuznetsova O, Laitem C, Zaborowska J, Dienstbier M, Murphy S. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids Res. 2014;42:264-75 pubmed publisher
    ..The Integrator complex substitutes for the mRNA cleavage and polyadenylation specificity factor complex to promote cleavage and couple snRNA 3'-end processing with termination...
  11. Chan S, Huppertz I, Yao C, Weng L, Moresco J, Yates J, et al. CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3' processing. Genes Dev. 2014;28:2370-80 pubmed publisher
    ..sites and, in mammals, is specifically recognized by the multisubunit CPSF (cleavage and polyadenylation specificity factor) complex...
  12. Lee K, Tarn W. TRAP150 activates splicing in composite terminal exons. Nucleic Acids Res. 2014;42:12822-32 pubmed publisher
    ..In this study, we found that TRAP150 interacted with the cleavage and polyadenylation specificity factor (CPSF) and co-fractionated with CPSF and RNA polymerase II...
  13. Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, et al. Position-specific binding of FUS to nascent RNA regulates mRNA length. Genes Dev. 2015;29:1045-57 pubmed publisher
    ..When an APA site is located upstream of an FUS cluster, FUS enhances polyadenylation by recruiting CPSF160 and up-regulates the alternative short transcript...
  14. Shimberg G, Pritts J, Michel S. Iron-Sulfur Clusters in Zinc Finger Proteins. Methods Enzymol. 2018;599:101-137 pubmed publisher
    ..Here, we describe methods to isolate and characterize one such ZF protein, cleavage and polyadenylation specificity factor 30 (CPSF3O) with respect to its metal-loading and RNA-binding activity.
  15. Liu M, Xu R, Merrill C, Hong L, Von Lanken C, Hunt A, et al. Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. PLoS ONE. 2014;9:e115779 pubmed publisher
    ..Previously, it was found that the 30 kD subunit of Arabidopsis cleavage and polyadenylation specificity factor (AtCPSF30) is a calmodulin-regulated RNA-binding protein...
  16. Merkel D, Wells S, Hilburn B, Elazzouzi F, Pérez Alvarado G, Lee B. The C-terminal region of cytoplasmic polyadenylation element binding protein is a ZZ domain with potential for protein-protein interactions. J Mol Biol. 2013;425:2015-2026 pubmed publisher
    ..of the ribonucleoprotein complex that includes the scaffold protein, Symplekin, and the cleavage and polyadenylation specificity factor. Sumoylation of Symplekin is required for polyadenylation, and both cleavage and polyadenylation ..
  17. Eifler T, Shao W, Bartholomeeusen K, Fujinaga K, Jäger S, Johnson J, et al. Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol Cell Biol. 2015;35:468-78 pubmed publisher
    ..II, as well as levels of cleavage-stimulating factor 64 (Cstf64) and 73-kDa subunit of cleavage and polyadenylation specificity factor (CPSF73), was reduced at the c-FOS gene...
  18. Rasaiyaah J, Tan C, Fletcher A, Price A, Blondeau C, Hilditch L, et al. HIV-1 evades innate immune recognition through specific cofactor recruitment. Nature. 2013;503:402-405 pubmed publisher
    ..HIV-1 capsid mutants N74D and P90A, which are impaired for interaction with cofactors cleavage and polyadenylation specificity factor subunit 6 (CPSF6) and cyclophilins (Nup358 and CypA), respectively, cannot replicate in primary ..
  19. Sowd G, Serrao E, Wang H, Wang W, Fadel H, Poeschla E, et al. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A. 2016;113:E1054-63 pubmed publisher
    ..roles in integration targeting, but the influence of another capsid binding protein, cleavage and polyadenylation specificity factor 6 (CPSF6), has not been reported...
  20. Sung T, Kim M, Kim T, Kim W, Park Y, Song D, et al. Negative Expression of CPSF2 Predicts a Poorer Clinical Outcome in Patients with Papillary Thyroid Carcinoma. Thyroid. 2015;25:1020-5 pubmed publisher
    ..A recent study reported that loss of the Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) gene was associated with increased cellular invasion, cancer stem cells, and ..
  21. Sasado T, Kondoh H, Furutani Seiki M, Naruse K. Mutation in cpsf6/CFIm68 (Cleavage and Polyadenylation Specificity Factor Subunit 6) causes short 3'UTRs and disturbs gene expression in developing embryos, as revealed by an analysis of primordial germ cell migration using the medaka mutant naruto. PLoS ONE. 2017;12:e0172467 pubmed publisher
    ..A fine genetic linkage mapping and genome sequencing showed the nar gene encodes Cleavage and Polyadenylation Specificity Factor subunit 6 (CPSF6/CFIm68)...
  22. DeDiego M, Nogales A, Lambert Emo K, Martinez Sobrido L, Topham D. NS1 Protein Mutation I64T Affects Interferon Responses and Virulence of Circulating H3N2 Human Influenza A Viruses. J Virol. 2016;90:9693-9711 pubmed publisher
    ..general inhibition of host protein synthesis by decreasing its interaction with cleavage and polyadenylation specificity factor 30 (CPSF30), leading to increased innate immune responses after viral infection...
  23. Nayeri S, Sargolzaei M, Abo Ismail M, May N, Miller S, Schenkel F, et al. Genome-wide association for milk production and female fertility traits in Canadian dairy Holstein cattle. BMC Genet. 2016;17:75 pubmed publisher
    ..some overlap with genes with known relevant biological functions identified in earlier studies such as DGAT1 and CPSF1. A significant region on chromosome 21 overlapping with the gene FAM181A and not previous linked to fertility in ..
  24. Chakrabarti M, Hunt A. CPSF30 at the Interface of Alternative Polyadenylation and Cellular Signaling in Plants. Biomolecules. 2015;5:1151-68 pubmed publisher
    ..amount of research has been done in characterizing different subunits of so-called Cleavage and Polyadenylation Specificity Factor (CPSF)...
  25. Palencia A, Bougdour A, Brenier Pinchart M, Touquet B, Bertini R, Sensi C, et al. Targeting Toxoplasma gondii CPSF3 as a new approach to control toxoplasmosis. EMBO Mol Med. 2017;9:385-394 pubmed publisher
    ..to be resistant to AN3661 had mutations in TgCPSF3, which encodes a homologue of cleavage and polyadenylation specificity factor subunit 3 (CPSF-73 or CPSF3), an endonuclease involved in mRNA processing in eukaryotes...
  26. Lin J, Xu R, Wu X, Shen Y, Li Q. Role of cleavage and polyadenylation specificity factor 100: anchoring poly(A) sites and modulating transcription termination. Plant J. 2017;91:829-839 pubmed publisher
    CPSF100 is a core component of the cleavage and polyadenylation specificity factor (CPSF) complex for 3'-end formation of mRNA, but it still has no clear functional assignment...
  27. Nogales A, Martinez Sobrido L, Topham D, DeDiego M. NS1 Protein Amino Acid Changes D189N and V194I Affect Interferon Responses, Thermosensitivity, and Virulence of Circulating H3N2 Human Influenza A Viruses. J Virol. 2017;91: pubmed publisher
    ..expression, most probably because these mutations decreased the binding of NS1 to the cleavage and polyadenylation specificity factor 30 (CPSF30)...
  28. Rasheedi S, Shun M, Serrao E, Sowd G, Qian J, Hao C, et al. The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes. J Biol Chem. 2016;291:11809-19 pubmed publisher
    ..This requires cleavage and polyadenylation specificity factor 6 (CPSF6), a cellular protein involved in pre-mRNA 3' end processing that binds HIV-1 capsid and ..
  29. Shimberg G, Michalek J, Oluyadi A, Rodrigues A, Zucconi B, Neu H, et al. Cleavage and polyadenylation specificity factor 30: An RNA-binding zinc-finger protein with an unexpected 2Fe-2S cluster. Proc Natl Acad Sci U S A. 2016;113:4700-5 pubmed publisher
    Cleavage and polyadenylation specificity factor 30 (CPSF30) is a key protein involved in pre-mRNA processing. CPSF30 contains five Cys3His domains (annotated as "zinc-finger" domains)...
  30. ENGELMAN A, Singh P. Cellular and molecular mechanisms of HIV-1 integration targeting. Cell Mol Life Sci. 2018;75:2491-2507 pubmed publisher
    ..several proteins that are additionally implicated in virus nuclear import, including cleavage and polyadenylation specificity factor 6, to suppress integration into heterochromatin...
  31. Liu Y, Li S, Chen Y, Kimberlin A, Cahoon E, Yu B. snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis. PLoS Biol. 2016;14:e1002571 pubmed publisher
    ..The catalytic component of this complex is likely the cleavage and polyadenylation specificity factor 73 kDa-I (CSPF73-I), which is the nuclease cleaving the pre-mRNA 3' end...
  32. Li Q, Liu Z, Lu W, Liu M. Interplay between Alternative Splicing and Alternative Polyadenylation Defines the Expression Outcome of the Plant Unique OXIDATIVE TOLERANT-6 Gene. Sci Rep. 2017;7:2052 pubmed publisher
    ..a transcript coding for AtCPSF30, an Arabidopsis ortholog of 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. On the other hand, alternative splicing of Intron-2 generates a longer transcript encoding a ..
  33. Misra A, Ou J, Zhu L, Green M. Global analysis of CPSF2-mediated alternative splicing: Integration of global iCLIP and transcriptome profiling data. Genom Data. 2015;6:217-21 pubmed publisher
    ..Using a genome-wide RNA interference screening strategy, we identified cleavage and polyadenylation specificity factor (CPSF) and symplekin (SYMPK) as cofactors of the well-known splicing regulator RBFOX2...
  34. Kiehl S, Zimmermann T, Savai R, Pullamsetti S, Seeger W, Bartkuhn M, et al. Epigenetic silencing of downstream genes mediated by tandem orientation in lung cancer. Sci Rep. 2017;7:3896 pubmed publisher
    ..induction of an upstream gene led to a repression of RASSF1A through a process involving histone deacetylases and CPSF1. A tumor-specific increase in expression of histone deacetylases and CPSF1 was detected in lung cancer...
  35. Sonoiki E, Ng C, Lee M, Guo D, Zhang Y, Zhou Y, et al. A potent antimalarial benzoxaborole targets a Plasmodium falciparum cleavage and polyadenylation specificity factor homologue. Nat Commun. 2017;8:14574 pubmed publisher
    ..to AN3661 harboured point mutations in pfcpsf3, which encodes a homologue of mammalian cleavage and polyadenylation specificity factor subunit 3 (CPSF-73 or CPSF3)...
  36. Chen Y, Stuwe E, Luo Y, Ninova M, Le Thomas A, Rozhavskaya E, et al. Cutoff Suppresses RNA Polymerase II Termination to Ensure Expression of piRNA Precursors. Mol Cell. 2016;63:97-109 pubmed publisher
    ..cleavage of nascent RNA at poly(A) sites by interfering with recruitment of the cleavage and polyadenylation specificity factor (CPSF) complex. Cuff also protects processed transcripts from degradation by the exonuclease Rat1...
  37. Clerici M, Faini M, Muckenfuss L, Aebersold R, Jinek M. Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex. Nat Struct Mol Biol. 2018;25:135-138 pubmed publisher
    ..in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3...
  38. Nayeri S, Sargolzaei M, Abo Ismail M, Miller S, Schenkel F, Moore S, et al. Genome-wide association study for lactation persistency, female fertility, longevity, and lifetime profit index traits in Holstein dairy cattle. J Dairy Sci. 2017;100:1246-1258 pubmed publisher
    ..Previously proposed causative and candidate genes supported by this work include DGAT1, GRINA, and CPSF1, whereas new candidate genes are SLC2A4RG and THRB...
  39. Clerici M, Faini M, Aebersold R, Jinek M. Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex. elife. 2017;6: pubmed publisher
    ..of the hexanucleotide AAUAAA motif in the pre-mRNA polyadenylation signal by the cleavage and polyadenylation specificity factor (CPSF) complex...
  40. Reichert M. Proteome analysis of sheep B lymphocytes in the course of bovine leukemia virus-induced leukemia. Exp Biol Med (Maywood). 2017;242:1363-1375 pubmed publisher
    ..chain, zyxin, filamin-A, and vitamin D-binding protein were downregulated, whereas cleavage and polyadenylation specificity factor subunit 5, non-POU domain-containing octamer-binding protein and small glutamine-rich ..
  41. Keller W, Bienroth S, Lang K, Christofori G. Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA. EMBO J. 1991;10:4241-9 pubmed
    ..The entire hexamer signal is involved in binding of CPF since modification of any of its bases interferes with complex formation. ..
  42. Di Giammartino D, Li W, Ogami K, Yashinskie J, Hoque M, Tian B, et al. RBBP6 isoforms regulate the human polyadenylation machinery and modulate expression of mRNAs with AU-rich 3' UTRs. Genes Dev. 2014;28:2248-60 pubmed publisher
    ..Our results implicate RBBP6 and iso3 as novel regulators of 3' processing, especially of RNAs with AU-rich 3' UTRs. ..
  43. McCracken S, Lambermon M, Blencowe B. SRm160 splicing coactivator promotes transcript 3'-end cleavage. Mol Cell Biol. 2002;22:148-60 pubmed
    ..3'-end formation coupled to splicing, SRm160 was found to associate specifically with the cleavage polyadenylation specificity factor and to stimulate the 3'-end cleavage of splicing-active pre-mRNAs more efficiently than that of ..
  44. McCracken S, Fong N, Yankulov K, Ballantyne S, Pan G, Greenblatt J, et al. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature. 1997;385:357-61 pubmed
  45. Rozenblatt Rosen O, Nagaike T, FRANCIS J, Kaneko S, Glatt K, Hughes C, et al. The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. Proc Natl Acad Sci U S A. 2009;106:755-60 pubmed publisher
    ..Here, we show that Cdc73 physically associates with the cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) complexes that are required for the maturation of mRNA ..
  46. Saito A, Henning M, Serrao E, Dubose B, Teng S, Huang J, et al. Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo. J Virol. 2016;90:6918-6935 pubmed publisher
    Cleavage and polyadenylation specificity factor subunit 6 (CPSF6), a host factor that interacts with the HIV-1 capsid (CA) protein, is implicated in diverse functions during the early part of the HIV-1 life cycle, including uncoating, ..
  47. Sagawa F, Ibrahim H, Morrison A, Wilusz C, Wilusz J. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J. 2011;30:3994-4005 pubmed publisher
    ..Collectively, these data suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3' end processing with other aspects of nuclear mRNA maturation. ..
  48. Kaufmann I, Martin G, Friedlein A, Langen H, Keller W. Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. EMBO J. 2004;23:616-26 pubmed
    ..polyadenylation of mRNA precursors (pre-mRNAs) by poly(A) polymerase (PAP) depends on cleavage and polyadenylation specificity factor (CPSF)...
  49. Menon S, Tsuge T, Dohmae N, Takio K, Wei N. Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome. BMC Biochem. 2008;9:1 pubmed publisher
    ..As an established component of transcription and RNA processing complexes, we hypothesis that SAP130 may link CRL mediated ubiquitination to gene expression. ..
  50. Kim Y, Kang H, Jetten A. The Krüppel-like zinc finger protein Glis2 functions as a negative modulator of the Wnt/beta-catenin signaling pathway. FEBS Lett. 2007;581:858-64 pubmed
    ..Our results indicate that Glis2 interacts with beta-catenin and suggest that Glis2 functions as a negative modulator of beta-catenin/TCF-mediated transcription. ..
  51. Schul W, Groenhout B, Koberna K, Takagaki Y, Jenny A, Manders E, et al. The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA. EMBO J. 1996;15:2883-92 pubmed
    The cleavage stimulation factor (CstF), and the cleavage and polyadenylation specificity factor (CPSF) are necessary for 3'-terminal processing of polyadenylated mRNAs...
  52. Van Etten J, Nyquist M, Li Y, Yang R, Ho Y, Johnson R, et al. Targeting a Single Alternative Polyadenylation Site Coordinately Blocks Expression of Androgen Receptor mRNA Splice Variants in Prostate Cancer. Cancer Res. 2017;77:5228-5235 pubmed publisher
    ..Blocking this signal with morpholino technology or silencing of the polyadenylation factor CPSF1 caused a splice switch that inhibited expression of AR variants and blocked androgen-independent growth of CRPC ..
  53. Samiotaki M, Balatsos N, Courtis N, Tsiapalis C. Assignment of the 160-kDa subunit of cleavage and polyadenylation specificity factor (CPSF1) to human chromosome 8q24.23 by radiation hybrid mapping. Cytogenet Cell Genet. 2000;90:234-5 pubmed
  54. Holland L, Gauthier L, Bell Rogers P, Yankulov K. Distinct parts of minichromosome maintenance protein 2 associate with histone H3/H4 and RNA polymerase II holoenzyme. Eur J Biochem. 2002;269:5192-202 pubmed
    ..Point mutations within the 169-212 domain of MCM2 disrupted its interaction with pol II holoenzyme both in vitro and in vivo. This region is distinct from the previously characterized histone H3 binding domain of MCM2. ..
  55. Laishram R, Anderson R. The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA. EMBO J. 2010;29:4132-45 pubmed publisher
    ..Star-PAP directly associated with cleavage and polyadenylation specificity factor (CPSF) 160 and 73 subunits and also the targeted pre-mRNA...
  56. Nogales A, Chauché C, DeDiego M, Topham D, Parrish C, Murcia P, et al. The K186E Amino Acid Substitution in the Canine Influenza Virus H3N8 NS1 Protein Restores Its Ability To Inhibit Host Gene Expression. J Virol. 2017;91: pubmed publisher
    ..in position 186 (K186E) that resulted in NS1 binding to the 30-kDa subunit of the cleavage and polyadenylation specificity factor (CPSF30), a cellular protein involved in pre-mRNA processing...
  57. Jenny A, Keller W. Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor. Nucleic Acids Res. 1995;23:2629-35 pubmed
    3'-processing of mRNA precursors depends on several protein factors. One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor or as well as for the tail elongation reaction...
  58. Takagaki Y, Manley J. Complex protein interactions within the human polyadenylation machinery identify a novel component. Mol Cell Biol. 2000;20:1515-25 pubmed
    ..We show that multiple factors, including CstF, cleavage-polyadenylation specificity factor, and symplekin, can be isolated from cells as part of a large complex...
  59. Dantonel J, Murthy K, Manley J, Tora L. Transcription factor TFIID recruits factor CPSF for formation of 3' end of mRNA. Nature. 1997;389:399-402 pubmed
    ..now characterized an immunopurified TFIID complex which we unexpectedly find contains the cleavage-polyadenylation specificity factor (CPSF), one of the factors required for formation of the 3' end of messenger RNA...
  60. de Vries H, Rüegsegger U, Hubner W, Friedlein A, Langen H, Keller W. Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. EMBO J. 2000;19:5895-904 pubmed
    ..hClp1 interacts with CF I(m) and the cleavage and polyadenylation specificity factor CPSF, suggesting that it bridges these two 3' end processing factors within the cleavage complex.
  61. Maeda Y, Ito M, Harashima N, Nakatsura T, Hida N, Imai N, et al. Cleavage and polyadenylation specificity factor (CPSF)-derived peptides can induce HLA-A2-restricted and tumor-specific CTLs in the majority of gastrointestinal cancer patients. Int J Cancer. 2002;99:409-17 pubmed
    ..CTL line established from T cells infiltrating into colon cancer and report herein cleavage and polyadenylation specificity factor (CPSF) as a potent antigen holding peptides capable of inducing CTLs...
  62. Thuresson A, Astrom J, Astrom A, Gronvik K, Virtanen A. Multiple forms of poly(A) polymerases in human cells. Proc Natl Acad Sci U S A. 1994;91:979-83 pubmed
    ..Interestingly, tentative phosphorylation sites have been identified in this region, suggesting that phosphorylation/dephosphorylation may regulate the interaction between the two polyadenylylation factors PAP and CPSF. ..
  63. Köhn M, Ihling C, Sinz A, Krohn K, Hüttelmaier S. The Y3** ncRNA promotes the 3' end processing of histone mRNAs. Genes Dev. 2015;29:1998-2003 pubmed publisher
    We demonstrate that the Y3/Y3** noncoding RNAs (ncRNAs) bind to the CPSF (cleavage and polyadenylation specificity factor) and that Y3** associates with the 3' untranslated region (UTR) of histone pre-mRNAs...