CNOT7

Summary

Gene Symbol: CNOT7
Description: CCR4-NOT transcription complex subunit 7
Alias: CAF-1, CAF1, Caf1a, hCAF-1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, carbon catabolite repressor protein (CCR4)-associative factor 1
Species: human
Products:     CNOT7

Top Publications

  1. Clement S, Scheckel C, Stoecklin G, Lykke Andersen J. Phosphorylation of tristetraprolin by MK2 impairs AU-rich element mRNA decay by preventing deadenylase recruitment. Mol Cell Biol. 2011;31:256-66 pubmed publisher
    ..Thus, TTP may remain poised to rapidly reactivate deadenylation of bound transcripts to downregulate gene expression once the p38 MAPK pathway is deactivated. ..
  2. Sandler H, Kreth J, Timmers H, Stoecklin G. Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin. Nucleic Acids Res. 2011;39:4373-86 pubmed publisher
    ..the complex is required for messenger RNA (mRNA) turnover through its two associated deadenylases, Ccr4 and Caf1. Not1 is the largest protein of the Ccr4-Not complex and serves as a scaffold for other subunits of the complex...
  3. Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, et al. Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases. Genes Dev. 2007;21:3135-48 pubmed
    ..tail is the rate-limiting step in the degradation of most mRNAs, and two major mRNA deadenylase complexes--Caf1-Ccr4 and Pan2-Pan3--play central roles in this process, referred to as deadenylation...
  4. Rouault J, Prevot D, Berthet C, Birot A, Billaud M, Magaud J, et al. Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex. J Biol Chem. 1998;273:22563-9 pubmed
    ..Finally, we found that box B, one of two conserved domains which define the Btg family, plays a functional role, namely that it is essential to the Btg-mCaf1 interaction. ..
  5. Doidge R, Mittal S, Aslam A, Winkler G. Deadenylation of cytoplasmic mRNA by the mammalian Ccr4-Not complex. Biochem Soc Trans. 2012;40:896-901 pubmed publisher
    ..mammalian cells including two enzymatic deadenylase subunits: one DEDD (Asp-Glu-Asp-Asp)-type deadenylase (either CNOT7/human Caf1/Caf1a or CNOT8/human Pop2/Caf1b/Calif) and one EEP (endonuclease-exonuclease-phosphatase)-type enzyme (..
  6. Petit A, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, et al. The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. Nucleic Acids Res. 2012;40:11058-72 pubmed publisher
    ..The conserved core of the complex consists of a catalytic module comprising two deadenylases (CAF1/POP2 and CCR4a/b) and the NOT module, which contains at least NOT1, NOT2 and NOT3...
  7. Ikematsu N, Yoshida Y, Kawamura Tsuzuku J, Ohsugi M, Onda M, Hirai M, et al. Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases. Oncogene. 1999;18:7432-41 pubmed
    ..Intriguingly, the amino-terminal half of Tob2 as well as that of Tob was associated with a human homologue of yeast Caf1, a component of the CCR4 transcription factor complex. Moreover, Caf1 was associated with cyclin dependent kinases...
  8. Albert T, Lemaire M, van Berkum N, Gentz R, Collart M, Timmers H. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res. 2000;28:809-17 pubmed
    ..It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional proteins like yeast CAF1. Here we report the isolation of cDNAs encoding human NOT2, NOT3, NOT4 and a CAF1-like factor, CALIF...
  9. Aslam A, Mittal S, Koch F, Andrau J, Winkler G. The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation. Mol Biol Cell. 2009;20:3840-50 pubmed publisher
    ..Here, we focused on the role of the human paralogues CNOT7 (hCaf1/Caf1a) and CNOT8 (hPop2/Caf1b/Calif), which possess deadenylase activity mediated by DEDD nuclease domains...

More Information

Publications95

  1. Morita M, Suzuki T, Nakamura T, Yokoyama K, Miyasaka T, Yamamoto T. Depletion of mammalian CCR4b deadenylase triggers elevation of the p27Kip1 mRNA level and impairs cell growth. Mol Cell Biol. 2007;27:4980-90 pubmed
    ..Thus, CCR4b may be involved in various cellular events that include cell proliferation. ..
  2. Miyasaka T, Morita M, Ito K, Suzuki T, Fukuda H, Takeda S, et al. Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex. Cancer Sci. 2008;99:755-61 pubmed publisher
    ..These results suggest that the antiproliferative activity of Tob is shown post-transcriptionally by controlling the stability of the target mRNAs in addition to its involvement in transcriptional regulation, reported previously. ..
  3. Draper M, Salvadore C, Denis C. Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex. Mol Cell Biol. 1995;15:3487-95 pubmed
    ..Both yCAF1 (POP2) and the C. elegans homolog of CAF1 were shown to genetically interact with CCR4 in vivo, and yCAF1 (POP2) physically associated with CCR4...
  4. Fabian M, Cieplak M, Frank F, Morita M, Green J, Srikumar T, et al. miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT. Nat Struct Mol Biol. 2011;18:1211-7 pubmed publisher
    ..Thus, GW182 serves as both a platform that recruits deadenylases and as a deadenylase coactivator that facilitates the removal of the poly(A) tail by CCR4-NOT. ..
  5. Marchese F, Aubareda A, Tudor C, Saklatvala J, Clark A, Dean J. MAPKAP kinase 2 blocks tristetraprolin-directed mRNA decay by inhibiting CAF1 deadenylase recruitment. J Biol Chem. 2010;285:27590-600 pubmed publisher
    ..b>CAF1a and CAF1b were found to interact with TTP in an RNA-independent fashion...
  6. Piao X, Zhang X, Wu L, Belasco J. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells. Mol Cell Biol. 2010;30:1486-94 pubmed publisher
    ..Overproducing a mutationally inactivated form of either of the catalytic subunits of this deadenylase (CCR4 or CAF1/POP2) significantly impedes the deadenylation and decay of mRNA targeted by a partially complementary miRNA...
  7. Lau N, Kolkman A, van Schaik F, Mulder K, Pijnappel W, Heck A, et al. Human Ccr4-Not complexes contain variable deadenylase subunits. Biochem J. 2009;422:443-53 pubmed publisher
    ..Taken together, human Ccr4-Not complexes are heterogeneous in composition owing to differences in their deadenylase subunits, which may reflect the multi-functionality of these complexes in cellular processes. ..
  8. Winkler G. The mammalian anti-proliferative BTG/Tob protein family. J Cell Physiol. 2010;222:66-72 pubmed publisher
    ..which is capable of binding to DNA-binding transcription factors as well as the paralogues CNOT7 (human Caf1/Caf1a) and CNOT8 (human Pop2/Calif/Caf1b), two deadenylase subunits of the Ccr4-Not complex...
  9. Ruan L, Osawa M, Hosoda N, Imai S, Machiyama A, Katada T, et al. Quantitative characterization of Tob interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation. J Biol Chem. 2010;285:27624-31 pubmed publisher
    ..is the first and often the rate-limiting step of eukaryotic mRNA decay in which two deadenylases, Ccr4-Caf1 and Pan2, play key roles...
  10. Doidge R, Mittal S, Aslam A, Winkler G. The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex. PLoS ONE. 2012;7:e51331 pubmed publisher
    ..This domain mediates interactions with the highly similar Caf1a (CNOT7) and Caf1b (CNOT8) catalytic subunits of the Ccr4-Not deadenylase complex...
  11. Ezzeddine N, Chen C, Shyu A. Evidence providing new insights into TOB-promoted deadenylation and supporting a link between TOB's deadenylation-enhancing and antiproliferative activities. Mol Cell Biol. 2012;32:1089-98 pubmed publisher
    ..A recruitment model was proposed in which TOBs promote deadenylation by recruiting CAF1-CCR4 deadenylase complex to the 3' end of mRNAs by simultaneously binding CAF1 and PABP...
  12. Braun J, Huntzinger E, Fauser M, Izaurralde E. GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Mol Cell. 2011;44:120-33 pubmed publisher
    ..We found that human GW182 proteins recruit the PAN2-PAN3 and CCR4-CAF1-NOT deadenylase complexes through direct interactions with PAN3 and NOT1, respectively...
  13. Hosoda N, Funakoshi Y, Hirasawa M, Yamagishi R, Asano Y, Miyagawa R, et al. Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase. EMBO J. 2011;30:1311-23 pubmed publisher
    ..Tob is involved in the general mechanism of mRNA decay by mediating mRNA deadenylation through interaction with Caf1 and a general RNA-binding protein, PABPC1. Here, we focus on the role of Tob in the regulation of specific mRNA...
  14. Bogdan J, Adams Burton C, Pedicord D, Sukovich D, Benfield P, Corjay M, et al. Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1. Biochem J. 1998;336 ( Pt 2):471-81 pubmed
  15. Horiuchi M, Takeuchi K, Noda N, Muroya N, Suzuki T, Nakamura T, et al. Structural basis for the antiproliferative activity of the Tob-hCaf1 complex. J Biol Chem. 2009;284:13244-55 pubmed publisher
    ..These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D (RNase D) family of deadenylases and is a component of the CCR4-Not ..
  16. Chalabi Hagkarim N, Ryan E, Byrd P, Hollingworth R, Shimwell N, Agathanggelou A, et al. Degradation of a Novel DNA Damage Response Protein, Tankyrase 1 Binding Protein 1, following Adenovirus Infection. J Virol. 2018;92: pubmed publisher
    ..The levels of at least 2 other members of the complex (CNOT3 and CNOT7) are also reduced during adenovirus infection, whereas the levels of CNOT4 and CNOT1 remain stable...
  17. Alhusaini N, Coller J. The deadenylase components Not2p, Not3p, and Not5p promote mRNA decapping. RNA. 2016;22:709-21 pubmed publisher
    ..The highly conserved CCR4/NOT deadenylase complex consists of the exonucleases CCR4 and POP2/CAF1, as well as a group of four or five (depending on organism) accessory factors of unknown function, i.e...
  18. Calvo Martín J, Librado P, Aguadé M, Papaceit M, Segarra C. Adaptive selection and coevolution at the proteins of the Polycomb repressive complexes in Drosophila. Heredity (Edinb). 2016;116:213-23 pubmed publisher
    ..The observed distribution of divergence differs substantially among and along proteins. Indeed, CAF1 is uniformly conserved, whereas only the established protein domains are conserved in other proteins, such as PHO, ..
  19. Valenzuela Miranda D, Nuñez Acuña G, Valenzuela Muñoz V, Asgari S, Gallardo Escárate C. MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): emerging role in delousing drug response. Gene. 2015;555:231-41 pubmed publisher
    ..In contrast, the subunit 7 (CNOT7) showed an inverse expression pattern...
  20. Volk A, Liang K, Suraneni P, Li X, Zhao J, Bulic M, et al. A CHAF1B-Dependent Molecular Switch in Hematopoiesis and Leukemia Pathogenesis. Cancer Cell. 2018;34:707-723.e7 pubmed publisher
    CHAF1B is the p60 subunit of the chromatin assembly factor (CAF1) complex, which is responsible for assembly of histones H3.1/H4 heterodimers at the replication fork during S phase...
  21. Faraji F, Hu Y, Yang H, Lee M, Winkler G, Hafner M, et al. Post-transcriptional Control of Tumor Cell Autonomous Metastatic Potential by CCR4-NOT Deadenylase CNOT7. PLoS Genet. 2016;12:e1005820 pubmed publisher
    ..and genetically engineered mouse models, we show that one of the enzymatic subunits of the CCR4-NOT complex, Cnot7, is also a metastasis modifying gene...
  22. Pavanello L, Hall B, Airhihen B, Winkler G. The central region of CNOT1 and CNOT9 stimulates deadenylation by the Ccr4-Not nuclease module. Biochem J. 2018;475:3437-3450 pubmed publisher
    ..include the nuclease module, which associates with the MIF4G domain of CNOT1 and contains the catalytic subunits Caf1 and Ccr4, as well as the module containing the non-catalytic CNOT9 subunit, which binds to the DUF3819 domain of ..
  23. Vargas D, De Bastiani M, Zimmer E, Klamt F. Alzheimer's disease master regulators analysis: search for potential molecular targets and drug repositioning candidates. Alzheimers Res Ther. 2018;10:59 pubmed publisher
    ..involvement in AD, such as ATF2 and PARK2, as well as possible new targets for future investigations, such as CNOT7, CSRNP2, SLC30A9, and TSC22D1...
  24. Nasertorabi F, Batisse C, Diepholz M, Suck D, Böttcher B. Insights into the structure of the CCR4-NOT complex by electron microscopy. FEBS Lett. 2011;585:2182-6 pubmed publisher
    ..The complex is conserved from yeast to human and consists of nine proteins NOT1-NOT5, CCR4, CAF1, CAF40 and CAF130...
  25. Yılmaz S. Effects of dietary caffeic acid supplement on antioxidant, immunological and liver gene expression responses, and resistance of Nile tilapia, Oreochromis niloticus to Aeromonas veronii. Fish Shellfish Immunol. 2019;86:384-392 pubmed publisher
    ..5 g were used in the study; three of them were fed with caffeic acid incorporated diets (1 g kg-1-Caf1, 5 g kg-1-Caf5, 10 g kg-1-Caf10), whereas an additive free basal diet served as the ..
  26. Durá G, Waller H, Gentile P, Lakey J, Fulton D. Tuneable hydrogels of Caf1 protein fibers. Mater Sci Eng C Mater Biol Appl. 2018;93:88-95 pubmed publisher
    Capsular antigen fraction 1 (Caf1) is a robust polymeric protein forming a protective layer around the bacterium Yersinia pestis...
  27. Takahashi A, Adachi S, Morita M, Tokumasu M, Natsume T, Suzuki T, et al. Post-transcriptional Stabilization of Ucp1 mRNA Protects Mice from Diet-Induced Obesity. Cell Rep. 2015;13:2756-67 pubmed publisher
    ..adipose tissue (iWAT) of mice fed a high-fat diet (HFD), Ucp1 level decreases concomitantly with increases in Cnot7 and its interacting partner Tob...
  28. Sun L, Sun G, Shi C, Sun D. Transcriptome analysis reveals new microRNAs-mediated pathway involved in anther development in male sterile wheat. BMC Genomics. 2018;19:333 pubmed publisher
    ..Noticeably, the largest miRNA family was MiR1122. The target CCR4-associated factor 1 (CAF1) of miR2275, a subunit of the Carbon Catabolite Repressed 4-Negative on TATA-less (CCR4-NOT) complex, contributes ..
  29. Ma J, Fukuda Y, Schultz R. Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation. Biol Reprod. 2015;93:48 pubmed publisher
    ..We report here that Cnot7, Cnot6l, and Pan2, key components of deadenylation machinery, are also dormant maternal mRNAs that are recruited ..
  30. Li M, Xu X, Chang C, Zheng L, Shen B, Liu Y. SUMO2 conjugation of PCNA facilitates chromatin remodeling to resolve transcription-replication conflicts. Nat Commun. 2018;9:2706 pubmed publisher
    ..Proteomic analysis reveals that SUMO2-PCNA enriches histone chaperones CAF1 and FACT in the replication complex via interactions with their SUMO-interacting motifs...
  31. Fennessy R, Owen Hughes T. Establishment of a promoter-based chromatin architecture on recently replicated DNA can accommodate variable inter-nucleosome spacing. Nucleic Acids Res. 2016;44:7189-203 pubmed publisher
    ..When the supply of histones is disrupted through mutation of the chaperone Caf1, a promoter-based architecture is generated, but with increased inter-nucleosomal spacing...
  32. Tripathi A, Singh K, Pareek A, Singla Pareek S. Histone chaperones in Arabidopsis and rice: genome-wide identification, phylogeny, architecture and transcriptional regulation. BMC Plant Biol. 2015;15:42 pubmed publisher
    ..These could be classified into seven different families, namely NAP, CAF1, SPT6, ASF1, HIRA, NASP, and FACT...
  33. Collart M, Panasenko O. The Ccr4-Not Complex: Architecture and Structural Insights. Subcell Biochem. 2017;83:349-379 pubmed publisher
    ..It has a conserved core of three Not proteins, the Ccr4 protein, and two Ccr4 associated factors, Caf1 and Caf40. A fourth Not protein, Not4, is conserved, but is only a stable subunit of the complex in yeast...
  34. Codutti L, Leppek K, Zálešák J, Windeisen V, Masiewicz P, Stoecklin G, et al. A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin. Structure. 2015;23:1437-1447 pubmed publisher
    ..The protein Roquin recognizes CDE-type stem loops and recruits the Ccr4-Caf1-Not deadenylase complex to the mRNA, thereby inducing its decay...
  35. Zaidan N, Walker K, Brown J, Schaffer L, Scalf M, Shortreed M, et al. Compartmentalization of HP1 Proteins in Pluripotency Acquisition and Maintenance. Stem Cell Reports. 2018;10:627-641 pubmed publisher
    ..HP1α and HP1γ complexes in ESCs differed in association with histones, the histone chaperone CAF1 complex, and specific components of chromatin-modifying complexes such as DPY30, implying distinct functional ..
  36. Cotobal C, Rodríguez López M, Duncan C, Hasan A, Yamashita A, Yamamoto M, et al. Role of Ccr4-Not complex in heterochromatin formation at meiotic genes and subtelomeres in fission yeast. Epigenetics Chromatin. 2015;8:28 pubmed publisher
    ..We show here that Ccr4-Not is recruited by Mmi1 to its RNA targets. Surprisingly, Ccr4 and Caf1 (the mRNA deadenylase catalytic subunits of the Ccr4-Not complex) are not necessary for the degradation or ..
  37. Kopylov P, Platonov M, Ablamunits V, Kombarova T, Ivanov S, Kadnikova L, et al. Yersinia pestis Caf1 Protein: Effect of Sequence Polymorphism on Intrinsic Disorder Propensity, Serological Cross-Reactivity and Cross-Protectivity of Isoforms. PLoS ONE. 2016;11:e0162308 pubmed publisher
    Yersinia pestis Caf1 is a multifunctional protein responsible for antiphagocytic activity and is a key protective antigen. It is generally conserved between globally distributed Y. pestis strains, but Y. pestis subsp...
  38. Chen X, Zhang X, Hu L, Zhang J, Jiang Y, Yang Y, et al. DsCaf1 is involved in environmental stress response of Dunaliella salina. Int J Biol Macromol. 2016;82:369-74 pubmed publisher
    ..In this research, we cloned the deadenylase caf1 from Dunaliella salina (dscaf1), a unicellular green alga with exceptional halotolerance...
  39. Chen C, Zhang Y, Xiang Y, Han L, Chang J, Shyu A. Antagonistic actions of two human Pan3 isoforms on global mRNA turnover. RNA. 2017;23:1404-1418 pubmed publisher
    ..Mammalian deadenylation exhibits biphasic kinetics, with the Pan2-Pan3 and Ccr4-Caf1 deadenylase complexes mediating the first and second phase, respectively; however, the significance of the biphasic ..
  40. Sun W, Sanapala S, Rahav H, Curtiss R. Oral administration of a recombinant attenuated Yersinia pseudotuberculosis strain elicits protective immunity against plague. Vaccine. 2015;33:6727-35 pubmed publisher
    A Yersinia pseudotuberculosis PB1+ (Yptb PB1+) mutant strain combined with chromosome insertion of the caf1R-caf1A-caf1M-caf1 operon and deletions of yopJ and yopK, χ10068 [pYV-ω2 (ΔyopJ315 ΔyopK108) ΔlacZ044::caf1R-..
  41. Chou W, Chung Y, Fang J, Lu C. Novel interaction between CCR4 and CAF1 in rice CCR4-NOT deadenylase complex. Plant Mol Biol. 2017;93:79-96 pubmed publisher
    ..The CCR4-NOT1 complex contains two key components, CCR4 and CAF1, which are the main cytoplasmic deadenylases in eukaryotic cells...
  42. Jin H, Li M, Duan S, Fu M, Dong X, Liu B, et al. Optimization of Light-Harvesting Pigment Improves Photosynthetic Efficiency. Plant Physiol. 2016;172:1720-1731 pubmed
    ..HPE1 also interacts with other plastid RNA-splicing factors, including CAF1 and OTP51, which share common targets with HPE1...
  43. Schmähling S, Meiler A, Lee Y, Mohammed A, Finkl K, Tauscher K, et al. Regulation and function of H3K36 di-methylation by the trithorax-group protein complex AMC. Development. 2018;145: pubmed publisher
    ..We show that Ash1 protein purified from Drosophila exists in a complex with MRG15 and Caf1 that we named AMC...
  44. Pasternak M, Pfender S, Santhanam B, Schuh M. The BTG4 and CAF1 complex prevents the spontaneous activation of eggs by deadenylating maternal mRNAs. Open Biol. 2016;6: pubmed publisher
    ..Here, we report that a complex of BTG4 and CAF1 safeguards metaphase II arrest in mammalian eggs by deadenylating maternal mRNAs...
  45. Sharma S, Poetz F, Bruer M, Ly Hartig T, Schott J, Seraphin B, et al. Acetylation-Dependent Control of Global Poly(A) RNA Degradation by CBP/p300 and HDAC1/2. Mol Cell. 2016;63:927-38 pubmed publisher
    ..Acetylation-induced RNA decay depends on the HATs p300 and CBP, which acetylate the exoribonuclease CAF1a, a catalytic subunit of the CCR4-CAF1-NOT deadenlyase complex and thereby contribute to accelerating poly(A) RNA ..
  46. Endo A, Ly T, Pippa R, Bensaddek D, Nicolas A, Lamond A. The Chromatin Assembly Factor Complex 1 (CAF1) and 5-Azacytidine (5-AzaC) Affect Cell Motility in Src-transformed Human Epithelial Cells. J Biol Chem. 2017;292:172-184 pubmed publisher
    ..for chromatin regulators acting downstream of activated Src identified the replication-dependent histone chaperone CAF1 as an important factor for Src-mediated increased cell motility and invasion...
  47. Yu C, Ji S, Sha Q, Dang Y, Zhou J, Zhang Y, et al. BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol. 2016;23:387-94 pubmed publisher
    ..Here, we identified B-cell translocation gene-4 (BTG4) as an MZT licensing factor in mice. BTG4 bridged CNOT7, a catalytic subunit of the CCR4-NOT deadenylase, to eIF4E, a key translation initiation factor, and facilitated ..
  48. Halter D, Collart M, Panasenko O. The Not4 E3 ligase and CCR4 deadenylase play distinct roles in protein quality control. PLoS ONE. 2014;9:e86218 pubmed publisher
    ..Consistently, the deadenylation enzymes responsible for the rate-limiting step in eukaryotic mRNA degradation, Caf1 and Ccr4, are subunits of the Ccr4-Not complex. Another subunit of this complex is a RING E3 ligase, Not4...
  49. Reddy B, Shrestha S, Hart K, Liang X, Kemirembe K, Cui L, et al. A bioinformatic survey of RNA-binding proteins in Plasmodium. BMC Genomics. 2015;16:890 pubmed publisher
    ..Finally, Cytoscape was used to create protein-protein interaction network for CITH-Dozi and Caf1-CCR4-Not complexes...
  50. Pellerito C, Morana O, Ferrante F, Calvaruso G, Notaro A, Sabella S, et al. Synthesis, chemical characterization, computational studies and biological activity of new DNA methyltransferases (DNMTs) specific inhibitor. Epigenetic regulation as a new and potential approach to cancer therapy. J Inorg Biochem. 2015;150:18-27 pubmed publisher
    ..deals with the synthesis, the chemical characterization of dibutyltin(IV) complex of caffeic acid (Bu2Sn(IV)HCAF, caf1) and its cytotoxic action on tumor cells...
  51. Levy R, Mott R, Iraqi F, Gabet Y. Collaborative cross mice in a genetic association study reveal new candidate genes for bone microarchitecture. BMC Genomics. 2015;16:1013 pubmed publisher
    ..10(-6)) and which have a validated causal role in skeletal biology, are Avp, Oxt, B2m (associated with BV/TV), Cnot7 (with Tb.N), Pcsk6, Rgma (with Tb.Th), Rb1, and Cpb2 (with Conn.D)...
  52. Dunning Hotopp J, Klasson L. The Complexities and Nuances of Analyzing the Genome of Drosophila ananassae and Its Wolbachia Endosymbiont. G3 (Bethesda). 2018;8:373-374 pubmed publisher
    ..i> (2017) improved contigs attributed to the Muller F element from the original CAF1 assembly, and used them to conclude that most of the sequence expansion of the fourth chromosome of D...
  53. Sauer P, Timm J, Liu D, Sitbon D, Boeri Erba E, Velours C, et al. Insights into the molecular architecture and histone H3-H4 deposition mechanism of yeast Chromatin assembly factor 1. elife. 2017;6: pubmed publisher
    ..Here, we report that yeast chromatin assembly factor 1 (CAF1), a conserved histone chaperone complex that deposits H3-H4 during DNA replication, binds a single H3-H4 ..
  54. Waghray S, Williams C, Coon J, Wickens M. Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA. 2015;21:1335-45 pubmed publisher
    ..Repression often is associated with poly(A) removal. The deadenylase CAF1 is a core component of the CCR4-NOT complex...
  55. Sha J, Kirtley M, Klages C, Erova T, Telepnev M, Ponnusamy D, et al. A Replication-Defective Human Type 5 Adenovirus-Based Trivalent Vaccine Confers Complete Protection against Plague in Mice and Nonhuman Primates. Clin Vaccine Immunol. 2016;23:586-600 pubmed publisher
    Currently, no plague vaccine exists in the United States for human use. The capsular antigen (Caf1 or F1) and two type 3 secretion system (T3SS) components, the low-calcium-response V antigen (LcrV) and the needle protein YscF, represent ..
  56. Stupfler B, Birck C, Séraphin B, Mauxion F. BTG2 bridges PABPC1 RNA-binding domains and CAF1 deadenylase to control cell proliferation. Nat Commun. 2016;7:10811 pubmed publisher
    ..BTG2 interacts with CAF1 deadenylase through its APRO domain, a defining feature of BTG/Tob factors...
  57. Niinuma S, Fukaya T, Tomari Y. CCR4 and CAF1 deadenylases have an intrinsic activity to remove the post-poly(A) sequence. RNA. 2016;22:1550-9 pubmed publisher
    MicroRNAs (miRNAs) recruit the CCR4-NOT complex, which contains two deadenylases, CCR4 and CAF1, to promote shortening of the poly(A) tail...
  58. Du H, Zhao Y, He J, Zhang Y, Xi H, Liu M, et al. YTHDF2 destabilizes m(6)A-containing RNA through direct recruitment of the CCR4-NOT deadenylase complex. Nat Commun. 2016;7:12626 pubmed publisher
    ..of the CNOT1 subunit, and that this recruitment is essential for the deadenylation of m(6)A-containing RNAs by CAF1 and CCR4...
  59. Cano F, Rapiteanu R, Sebastiaan Winkler G, Lehner P. A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C. Nat Commun. 2015;6:8670 pubmed publisher
    ..family of RNA-binding ubiquitin ligases, associates with the cytoplasmic deadenylation complexes and ubiquitinates CNOT7(Caf1), the main catalytic subunit of the CCR4-NOT deadenylation machinery...
  60. Maragozidis P, Karangeli M, Labrou M, Dimoulou G, Papaspyrou K, Salataj E, et al. Alterations of deadenylase expression in acute leukemias: evidence for poly(a)-specific ribonuclease as a potential biomarker. Acta Haematol. 2012;128:39-46 pubmed publisher
    ..and control cells were analyzed for deadenylase mRNA levels using qRT-PCR, and the protein levels of PARN and CNOT7 deadenylases using immunoblotting...
  61. McFleder R, Mansur F, Richter J. Dynamic Control of Dendritic mRNA Expression by CNOT7 Regulates Synaptic Efficacy and Higher Cognitive Function. Cell Rep. 2017;20:683-696 pubmed publisher
    ..Here, we find that the deadenylase CNOT7 dynamically regulates dendritic mRNA translation and transport, as well as synaptic plasticity and higher ..
  62. Zhang Q, Yan D, Guo E, Ding B, Yang W, Liu R, et al. Structural basis for inhibition of the deadenylase activity of human CNOT6L. FEBS Lett. 2016;590:1270-9 pubmed publisher
    ..Deadenylase activity assays show that nucleotides are effective inhibitors of both CNOT6L and CNOT7, with AMP more effective than other nucleotides, and that neomycin is a weak deadenylase inhibitor...
  63. Robin Lespinasse Y, Sentis S, Kolytcheff C, Rostan M, Corbo L, Le Romancer M. hCAF1, a new regulator of PRMT1-dependent arginine methylation. J Cell Sci. 2007;120:638-47 pubmed
    ..Since hCAF1 and PRMT1 have been involved in the regulation of transcription and RNA metabolism, we speculate that hCAF1 and PRMT1 could contribute to the crosstalk between transcription and RNA processing. ..
  64. Adachi S, Homoto M, Tanaka R, Hioki Y, Murakami H, Suga H, et al. ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway. Nucleic Acids Res. 2014;42:10037-49 pubmed publisher
    ..Our results also show the usefulness of our method for identifying critical regulators of specific RNAs and the potency of antisense oligonucleotide-based therapeutics. ..
  65. Jadhav G, Kaur I, Maryati M, Airhihen B, Fischer P, Winkler G. Discovery, synthesis and biochemical profiling of purine-2,6-dione derivatives as inhibitors of the human poly(A)-selective ribonuclease Caf1. Bioorg Med Chem Lett. 2015;25:4219-24 pubmed publisher
    ..involved in the shortening of the poly(A) tail include the multi-subunit Ccr4-Not deadenylase, which contains the Caf1 (Pop2) and Ccr4 catalytic components, and poly(A)-specific ribonuclease (PARN)...
  66. Chapat C, Kolytcheff C, Le Romancer M, Auboeuf D, de la Grange P, Chettab K, et al. hCAF1/CNOT7 regulates interferon signalling by targeting STAT1. EMBO J. 2013;32:688-700 pubmed publisher
    ..The transcription factor STAT1 is a crucial mediator of this response. Here, we show that hCAF1/CNOT7 regulates class I and II IFN pathways at different crucial steps...
  67. Chapat C, Chettab K, Simonet P, Wang P, de la Grange P, Le Romancer M, et al. Alternative splicing of CNOT7 diversifies CCR4-NOT functions. Nucleic Acids Res. 2017;45:8508-8523 pubmed publisher
    The CCR4-associated factor CAF1, also called CNOT7, is a catalytic subunit of the CCR4-NOT complex, which has been implicated in all aspects of the mRNA life cycle, from mRNA synthesis in the nucleus to degradation in the cytoplasm...
  68. Fabian M, Mathonnet G, Sundermeier T, Mathys H, Zipprich J, Svitkin Y, et al. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009;35:868-80 pubmed publisher
    ..let-7 miRNA-loaded RNA-induced silencing complex (miRISC) interacts with the poly(A)-binding protein (PABP) and the CAF1 and CCR4 deadenylases...
  69. Van Etten J, Schagat T, Hrit J, Weidmann C, Brumbaugh J, Coon J, et al. Human Pumilio proteins recruit multiple deadenylases to efficiently repress messenger RNAs. J Biol Chem. 2012;287:36370-83 pubmed publisher
    ..First, dominant-negative mutants of CNOT7 and CNOT8 reduced PUM repression. Second, RNA interference depletion of the deadenylases alleviated PUM repression...
  70. Yoshida Y, Hosoda E, Nakamura T, Yamamoto T. Association of ANA, a member of the antiproliferative Tob family proteins, with a Caf1 component of the CCR4 transcriptional regulatory complex. Jpn J Cancer Res. 2001;92:592-6 pubmed
    ..of these, except ANA and PC3B, have been shown to interact with the CCR4 transcription factor-associated protein Caf1. Here we show that ANA also associates with Caf1, ANA being the preferred partner of Caf1 among the Tob family ..
  71. Nishida K, Horiuchi M, Noda N, Takahasi K, Iwasaki N, Minami A, et al. Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:1061-3 pubmed
    ..These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases...
  72. Zukeran A, Takahashi A, Takaoka S, Mohamed H, Suzuki T, Ikematsu S, et al. The CCR4-NOT deadenylase activity contributes to generation of induced pluripotent stem cells. Biochem Biophys Res Commun. 2016;474:233-239 pubmed publisher
    ..Here, we show that the CCR4-NOT deadenylase subunits Cnot6, Cnot6l, Cnot7, and Cnot8, participate in regulating iPSC generation...
  73. Maragozidis P, Papanastasi E, Scutelnic D, Totomi A, Kokkori I, Zarogiannis S, et al. Poly(A)-specific ribonuclease and Nocturnin in squamous cell lung cancer: prognostic value and impact on gene expression. Mol Cancer. 2015;14:187 pubmed publisher
    ..Quantitative real-time PCR showed that four deadenylases, PARN, CNOT6, CNOT7 and NOC, are differentially expressed in our SCC clinical samples...
  74. Prevot D, Morel A, Voeltzel T, Rostan M, Rimokh R, Magaud J, et al. Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway. J Biol Chem. 2001;276:9640-8 pubmed
    ..previously the physical interaction of B-cell translocation gene proteins (BTG)1 and BTG2 with the mouse protein CAF1 (CCR4-associated factor 1) and suggested that these proteins may participate, through their association with CAF1, ..
  75. Shen M, Wu M, Chen L, Zhi Q, Gong F, Chen K, et al. Cantharidin represses invasion of pancreatic cancer cells through accelerated degradation of MMP2 mRNA. Sci Rep. 2015;5:11836 pubmed publisher
    ..Knockdown of PARN, RHAU, and CNOT7, three critical members involved in cytoplasmic deadenylation, attenuated the downregulation of MMP2...
  76. Gupta M, Agarawal M, Banu K, Reddy K, Gaur D, Dhar S. Role of Chromatin assembly factor 1 in DNA replication of Plasmodium falciparum. Biochem Biophys Res Commun. 2018;495:1285-1291 pubmed publisher
    ..Here, we report the functional characterization of P. falciparum chromatin assembly factor 1 (CAF1), which interacts with several proteins namely PfCAF2, Histones, PfHP1 and others...
  77. Maryati M, Kaur I, Jadhav G, Olotu Umoren L, Oveh B, Hashmi L, et al. A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity. Nucleic Acids Res. 2014;42:e30 pubmed publisher
    ..by screening a chemical compound library, resulting in the identification of non-nucleoside inhibitors of the Caf1/CNOT7 enzyme, a catalytic subunit of the Ccr4-Not deadenylase complex...
  78. Flanagan J, Healey S, Young J, Whitehall V, Chenevix Trench G. Analysis of the transcription regulator, CNOT7, as a candidate chromosome 8 tumor suppressor gene in colorectal cancer. Int J Cancer. 2003;106:505-9 pubmed
    ..b>CNOT7 encodes a subunit of the CCR4-Not transcription complex and is located at 8p22...
  79. Maryati M, Airhihen B, Winkler G. The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module. Biochem J. 2015;469:169-76 pubmed publisher
    ..is encoded by two paralogues: Caf1, which can interact with the anti-proliferative protein BTG2, is encoded by CNOT7 and CNOT8, whereas Ccr4 is encoded by the highly similar genes CNOT6 and CNOT6L...
  80. Mishima Y, Tomari Y. Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish. Genes Cells. 2017;22:670-678 pubmed publisher
    ..Interestingly, each mRNA showed differential dependency on Dcp2-mediated decapping and Cnot7-mediated deadenylation for degradation...
  81. Winkler G, Balacco D. Heterogeneity and complexity within the nuclease module of the Ccr4-Not complex. Front Genet. 2013;4:296 pubmed publisher
    ..complexity of the complex is further increased by the presence of paralogs of the Caf1 subunit (encoded by either CNOT7 or CNOT8) and the occurrence of two Ccr4 paralogs (encoded by CNOT6 or CNOT6L)...
  82. Bianchin C, Mauxion F, Sentis S, Seraphin B, Corbo L. Conservation of the deadenylase activity of proteins of the Caf1 family in human. RNA. 2005;11:487-94 pubmed
    The yeast Pop2 protein, belonging to the eukaryotic Caf1 family, is required for mRNA deadenylation in vivo. It also catalyzes poly(A) degradation in vitro, even though this property has been questioned...
  83. Adams M, Dubnick M, Kerlavage A, Moreno R, Kelley J, Utterback T, et al. Sequence identification of 2,375 human brain genes. Nature. 1992;355:632-4 pubmed
    ..These data represent an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome. ..
  84. Shi J, Li J, Hu R, Shi Y, Su X, Li Q, et al. CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by tristetraprolin. Cell Signal. 2014;26:2390-6 pubmed publisher
    ..Observations have indicated that TTP can interact with CAF1 (CNOT7/hCAF1 in human), a subunit of the CCR4-NOT complex with deadenylase activity...
  85. Dupressoir A, Morel A, Barbot W, Loireau M, Corbo L, Heidmann T. Identification of four families of yCCR4- and Mg2+-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding. BMC Genomics. 2001;2:9 pubmed
  86. Bai Y, Tashiro S, Nagatoishi S, Suzuki T, Yan D, Liu R, et al. Structural basis for inhibition of the Tob-CNOT7 interaction by a fragment screening approach. Protein Cell. 2015;6:924-8 pubmed publisher