Gene Symbol: CLIC4
Description: chloride intracellular channel 4
Alias: CLIC4L, MTCLIC, huH1, p64H1, chloride intracellular channel protein 4, chloride intracellular channel 4 like, intracellular chloride ion channel protein p64H1
Species: human
Products:     CLIC4

Top Publications

  1. Suh K, Crutchley J, Koochek A, Ryscavage A, Bhat K, Tanaka T, et al. Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. Clin Cancer Res. 2007;13:121-31 pubmed
    b>CLIC4, a member of a family of intracellular chloride channels, is regulated by p53, c-Myc, and tumor necrosis factor-alpha...
  2. Bohman S, Matsumoto T, Suh K, Dimberg A, Jakobsson L, Yuspa S, et al. Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis. J Biol Chem. 2005;280:42397-404 pubmed
    ..We also identified regulation of chloride intracellular channel 4 (CLIC4), the expression of which decreased during tubular morphogenesis...
  3. Shiio Y, Suh K, Lee H, Yuspa S, Eisenman R, Aebersold R. Quantitative proteomic analysis of myc-induced apoptosis: a direct role for Myc induction of the mitochondrial chloride ion channel, mtCLIC/CLIC4. J Biol Chem. 2006;281:2750-6 pubmed
    ..tag quantitative proteomics approach and identified that a proapoptotic mitochondrial chloride ion channel, mtCLIC/CLIC4, is induced by Myc. Myc binds to the mtCLIC gene promoter and activates its transcription...
  4. Suh K, Malik M, Shukla A, Ryscavage A, Wright L, Jividen K, et al. CLIC4 is a tumor suppressor for cutaneous squamous cell cancer. Carcinogenesis. 2012;33:986-95 pubmed publisher
    ..In metabolically stressed skin keratinocytes, cytoplasmic CLIC4 is S-nitrosylated and translocates to the nucleus where it enhances transforming growth factor-? (TGF-?) signaling ..
  5. Duncan R, Westwood P, Boyd A, Ashley R. Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum. J Biol Chem. 1997;272:23880-6 pubmed
    ..approach to identify candidate genes in mammalian brain and cloned the cDNA corresponding to rat brain p64H1. This encoded a microsomal membrane protein of predicted Mr 28,635 homologous to the putative intracellular bovine ..
  6. Qian Z, Okuhara D, Abe M, Rosner M. Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel. J Biol Chem. 1999;274:1621-7 pubmed
    ..The specific association of CLIC3 with the COOH-terminal tail of ERK7 suggests that CLIC3 may play a role in the regulation of cell growth. ..
  7. Ponsioen B, van Zeijl L, Langeslag M, Berryman M, Littler D, Jalink K, et al. Spatiotemporal regulation of chloride intracellular channel protein CLIC4 by RhoA. Mol Biol Cell. 2009;20:4664-72 pubmed publisher
    ..However, its function(s) and regulation remain unclear. Here, we show that cytosolic CLIC4 undergoes rapid but transient translocation to discrete domains at the plasma membrane upon stimulation of G(13)-..
  8. Yao Q, Qu X, Yang Q, Wei M, Kong B. CLIC4 mediates TGF-beta1-induced fibroblast-to-myofibroblast transdifferentiation in ovarian cancer. Oncol Rep. 2009;22:541-8 pubmed
    ..Chloride intracellular channel 4 (CLIC4) may be functionally import for tumour stromal fibroblast-to-myofibroblast transdifferentiaton, but the molecular ..
  9. Suginta W, Karoulias N, Aitken A, Ashley R. Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms. Biochem J. 2001;359:55-64 pubmed
    ..To help in addressing these problems, we identified novel rat brain CLIC4 (p64H1) binding partners by affinity chromatography, mass spectrometric analysis and microsequencing...

More Information


  1. Rønnov Jessen L, Villadsen R, Edwards J, Petersen O. Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts. Am J Pathol. 2002;161:471-80 pubmed
    ..One of the clones, identified as CLIC4, a member of the CLIC family of chloride channels, was up-regulated more than 16 times in myofibroblasts and was ..
  2. Suh K, Mutoh M, Mutoh T, Li L, Ryscavage A, Crutchley J, et al. CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation. J Cell Sci. 2007;120:2631-40 pubmed
    ..We now show that Cl(-) intracellular channel 4 (CLIC4) expression is increased in both mouse and human keratinocytes undergoing differentiation induced by Ca(2+), serum ..
  3. Berryman M, Goldenring J. CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells. Cell Motil Cytoskeleton. 2003;56:159-72 pubmed
    b>CLIC4 is a member of the chloride intracellular channel (CLIC) protein family whose principal cellular functions are poorly understood. Recently, we demonstrated that several CLIC proteins, including CLIC4, interact with AKAP350...
  4. Li D, Li Y, Huang Q, Zhang Y, Wang D. Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein CLIC4. Protein Pept Lett. 2006;13:641-3 pubmed
    The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapour-diffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method...
  5. Argenzio E, Klarenbeek J, Kedziora K, Nahidiazar L, Isogai T, Perrakis A, et al. Profilin binding couples chloride intracellular channel protein CLIC4 to RhoA-mDia2 signaling and filopodium formation. J Biol Chem. 2018;293:19161-19176 pubmed publisher
    Chloride intracellular channel 4 (CLIC4) is a cytosolic protein implicated in diverse actin-based processes, including integrin trafficking, cell adhesion, and tubulogenesis...
  6. Zhu X, Lang H, Wang X, Hui S, Zhou M, Kang C, et al. Synergy between dihydromyricetin intervention and irinotecan chemotherapy delays the progression of colon cancer in mouse models. Food Funct. 2019;10:2040-2049 pubmed publisher
    ..Mechanically, chloride ion-dependent CFTR, CLCN4, and CLIC4 signaling are not involved in DMY mediated chemotherapeutic colon tumorigenesis...
  7. Costello A, Coleman O, Lao N, Henry M, Meleady P, Barron N, et al. Depletion of endogenous miRNA-378-3p increases peak cell density of CHO DP12 cells and is correlated with elevated levels of ubiquitin carboxyl-terminal hydrolase 14. J Biotechnol. 2018;288:30-40 pubmed publisher
    ..qRT-PCR of 8 genes; Clic4, Hnrnpa1, Prdx1, Actn4, Usp14, Srxn1, Canx and Gnb1, with unidirectional differential protein expression over the ..
  8. Li H, Zhang M, Linghu E, Zhou F, Herman J, Hu L, et al. Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma. Clin Epigenetics. 2018;10:137 pubmed publisher
    ..TMEM176A was highly expressed in SNU387, SNU182, Huh1, and SNU475 cells; reduced expression was observed in HepG2 and PLC/PRF/5 cells; and no expression was found in ..
  9. Singha B, Harper S, Goldman A, Bitler B, Aird K, Borowsky M, et al. CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer. Sci Rep. 2018;8:14725 pubmed publisher
    ..Here we investigated tissue staining and tissue secretion of CLIC1 and CLIC4 across EOC subtypes...
  10. Guo D, Xie W, Xiong P, Li H, Wang S, Chen G, et al. Cyclin-dependent kinase 5-mediated phosphorylation of chloride intracellular channel 4 promotes oxidative stress-induced neuronal death. Cell Death Dis. 2018;9:951 pubmed publisher
    ..immunofluorescence experiments and in vitro kinase assays, we identified chloride intracellular channel 4 (CLIC4), the expression of which increases during neuronal apoptosis, as a CDK5 substrate...
  11. Hara Y, Yamashita T, Oishi N, Nio K, Hayashi T, Nomura Y, et al. TSU-68 ameliorates hepatocellular carcinoma growth by inhibiting microenvironmental platelet-derived growth factor signaling. Anticancer Res. 2015;35:1423-31 pubmed
    ..HCC and fibroblast cell lines (HuH7, Hep3B, HuH1 and WI-38) were used to evaluate their interactions...
  12. Huang M, Chu I, Wang Z, Lin S, Chang T, Chen C. A G-Quadruplex Structure in the Promoter Region of CLIC4 Functions as a Regulatory Element for Gene Expression. Int J Mol Sci. 2018;19: pubmed publisher
    The differential transcriptional expression of CLIC4 between tumor cells and the surrounding stroma during cancer progression has been suggested to have a tumor-promoting effect...
  13. Shukla A, Malik M, Cataisson C, Ho Y, Friesen T, Suh K, et al. TGF-beta signalling is regulated by Schnurri-2-dependent nuclear translocation of CLIC4 and consequent stabilization of phospho-Smad2 and 3. Nat Cell Biol. 2009;11:777-84 pubmed publisher
    b>CLIC4 (chloride intracellular channel 4), a multifunctional protein that traffics between the cytoplasm and nucleus, interacts with Schnurri-2, a transcription factor in the bone morphogenetic protein (BMP) signalling pathway...
  14. Kongsune P, Hannongbua S. The role of conserved QXG and binding affinity of S23G & S26G receptors on avian H5, swine H1 and human H1 of influenza A virus hemagglutinin. J Mol Graph Model. 2018;82:12-19 pubmed publisher
    ..1?kcal/mol) while the relative energies of AvH5 and HuH1 were found to be significantly different (11.1?±?6.8 and 29.0?±?8.2?kcal/mol for AvH5 and HuH1, respectively).
  15. Lu J, Wu L, Wang X, Zhu J, Du J, Shen B. Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro. J Vis Exp. 2018;: pubmed publisher
    ..As one of the six members of the Cl- intracellular channel (CLIC) family, CLIC4 participates in the cell apoptotic process mainly through the mitochondrial pathway...
  16. Raedler D, Ballenberger N, Klucker E, Böck A, Otto R, Prazeres da Costa O, et al. Identification of novel immune phenotypes for allergic and nonallergic childhood asthma. J Allergy Clin Immunol. 2015;135:81-91 pubmed publisher
    ..Patients with AA had decreased expression of chloride intracellular channel 4 (CLIC4) and tuberous sclerosis 1 (TSC1), important innate immunity regulators...
  17. Liang J, De Castro A, Flores L. Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans. J Vis Exp. 2018;: pubmed publisher
    ..elegans, a member of the chloride intracellular channel proteins (CLICs) family, including mammalian CLIC4. An integrated translational exl-1::gfp transgenic line (with a promoter and a full gene sequence) was created by ..
  18. Patel D, Ythier D, Brozzi F, Eizirik D, Thorens B. Clic4, a novel protein that sensitizes β-cells to apoptosis. Mol Metab. 2015;4:253-64 pubmed publisher
    b>Chloride intracellular channel protein 4 (Clic4) is a ubiquitously expressed protein involved in multiple cellular processes including cell-cycle control, cell differentiation, and apoptosis...
  19. Lecat S, Matthes H, Pepperkok R, Simpson J, Galzi J. A Fluorescent Live Imaging Screening Assay Based on Translocation Criteria Identifies Novel Cytoplasmic Proteins Implicated in G Protein-coupled Receptor Signaling Pathways. Mol Cell Proteomics. 2015;14:1385-99 pubmed publisher
    ..CLIC2, and one of its close paralogs, CLIC4, were further shown to respond with the same translocation pattern to muscarinic M3 and lysophosphatidic LPA ..
  20. Chou S, Hsu K, Otsu W, Hsu Y, Luo Y, Yeh C, et al. CLIC4 regulates apical exocytosis and renal tube luminogenesis through retromer- and actin-mediated endocytic trafficking. Nat Commun. 2016;7:10412 pubmed publisher
    Chloride intracellular channel 4 (CLIC4) is a mammalian homologue of EXC-4 whose mutation is associated with cystic excretory canals in nematodes. Here we show that CLIC4-null mouse embryos exhibit impaired renal tubulogenesis...
  21. Ponnalagu D, Gururaja Rao S, Farber J, Xin W, Hussain A, Shah K, et al. Molecular identity of cardiac mitochondrial chloride intracellular channel proteins. Mitochondrion. 2016;27:6-14 pubmed publisher, we report the presence of unique dimorphic chloride intracellular channel (CLIC) proteins namely CLIC1, CLIC4 and CLIC5 as abundant CLICs in the rodent heart...
  22. Higashi T, Hayashi H, Ishimoto T, Takeyama H, Kaida T, Arima K, et al. miR-9-3p plays a tumour-suppressor role by targeting TAZ (WWTR1) in hepatocellular carcinoma cells. Br J Cancer. 2015;113:252-8 pubmed publisher
    ..Inversely, treatment of miR-9-3p inhibitor accelerated cell growth compared with control in HuH1. MicroRNA-9-3p was identified as the tumour-suppressor miR targetting TAZ expression in HCC cells.
  23. Ye M, Li J, Gong J. PCDH10 gene inhibits cell proliferation and induces cell apoptosis by inhibiting the PI3K/Akt signaling pathway in hepatocellular carcinoma cells. Oncol Rep. 2017;37:3167-3174 pubmed publisher
    ..PCDH10 expression was downregulated in the HCC cells (HepG2, HuH7, HuH1, and SNU387) when compared to the normal liver cells (L02)...
  24. Shukla A, Yang Y, Madanikia S, Ho Y, Li M, Sanchez V, et al. Elevating CLIC4 in Multiple Cell Types Reveals a TGF- Dependent Induction of a Dominant Negative Smad7 Splice Variant. PLoS ONE. 2016;11:e0161410 pubmed publisher
    b>CLIC4 (Chloride intracellular channel 4) belongs to a family of putative intracellular chloride channel proteins expressed ubiquitously in multiple tissues...
  25. Higashi T, Hayashi H, Kitano Y, Yamamura K, Kaida T, Arima K, et al. Statin attenuates cell proliferative ability via TAZ (WWTR1) in hepatocellular carcinoma. Med Oncol. 2016;33:123 pubmed
    ..TAZ expression level in HCC cell lines was analyzed by western blot. Two cell lines (HLF and HuH1) were used in this study...
  26. Lucitti J, Tarte N, Faber J. Chloride intracellular channel 4 is required for maturation of the cerebral collateral circulation. Am J Physiol Heart Circ Physiol. 2015;309:H1141-50 pubmed publisher
    ..Elsewhere, chloride intracellular protein 4 (CLIC4) was implicated but not investigated directly, prompting the present study...
  27. Zhang Y, Ma C, Zhao J, Xu H, Hou Q, Zhang H. Lactobacillus casei Zhang and vitamin K2 prevent intestinal tumorigenesis in mice via adiponectin-elevated different signaling pathways. Oncotarget. 2017;8:24719-24727 pubmed publisher Factor Kappa B(NF-κB) and intestinal Claudin15/Chloride intracellular channel 4(CLIC4)/Transforming Growth Factor Beta(TGF-β) signaling, while the vitamin K2 effect involved a hepatic Vitamin D ..
  28. Domingo Fernández R, Coll R, Kearney J, Breit S, O Neill L. The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1? transcription and activate the NLRP3 inflammasome. J Biol Chem. 2017;292:12077-12087 pubmed publisher
    ..Here, we report that the chloride intracellular channel proteins CLIC1 and CLIC4 participate in the regulation of the NLRP3 inflammasome...
  29. Xue H, Lu J, Yuan R, Liu J, Liu Y, Wu K, et al. Knockdown of CLIC4 enhances ATP-induced HN4 cell apoptosis through mitochondrial and endoplasmic reticulum pathways. Cell Biosci. 2016;6:5 pubmed publisher
    ..Chloride intracellular channel 4 (CLIC4), one of the seven members of the CLIC family, is a newly found Cl(-) channel that participates in various ..
  30. Guo Y, Peng Y, Gao D, Zhang M, Yang W, Linghu E, et al. Silencing HOXD10 by promoter region hypermethylation activates ERK signaling in hepatocellular carcinoma. Clin Epigenetics. 2017;9:116 pubmed publisher
    ..cell lines (SNU182, SNU449, HBXF344, SMMC7721, Huh7, HepG2, LM3, PLC/PRF/5, BEL7402, SNU387, SNU475, QGY7703, and Huh1) were included in this study...
  31. Stakaityte G, Nwogu N, Lippiat J, Blair G, Poterlowicz K, Boyne J, et al. The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility. J Biol Chem. 2018;293:4582-4590 pubmed publisher
    ..Proteomic analysis revealed that MCPyV ST up-regulates two Cl- channels, CLIC1 and CLIC4, which when silenced, inhibit MCPyV ST-induced motility and invasiveness, implicating their function as critical ..
  32. Nomura Y, Yamashita T, Oishi N, Nio K, Hayashi T, Yoshida M, et al. De Novo Emergence of Mesenchymal Stem-Like CD105+ Cancer Cells by Cytotoxic Agents in Human Hepatocellular Carcinoma. Transl Oncol. 2017;10:184-189 pubmed publisher
    ..The EpCAM+ cell lines HuH1 and HuH7 were treated with 5-fluorouracil (5-FU) or epirubicin in vitro...
  33. Tavasoli M, Al Momany A, Wang X, Li L, Edwards J, Ballermann B. Both CLIC4 and CLIC5A activate ERM proteins in glomerular endothelium. Am J Physiol Renal Physiol. 2016;311:F945-F957 pubmed publisher
    ..Since glomerular EC also express CLIC4, we reasoned that, if CLIC4 activates ERM proteins like CLIC5A, then CLIC4 could compensate for the CLIC5A loss in ..
  34. Mosbech A, Gibbs Seymour I, Kagias K, Thorslund T, Beli P, Povlsen L, et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol. 2012;19:1084-92 pubmed publisher
    ..Our findings establish DVC1 as a DNA damage-targeting p97 adaptor that protects cells from deleterious consequences of replication blocks and suggest an important role of p97 in ubiquitin-dependent regulation of TLS...
  35. Wang G, Bi L, Wang G, Huang F, Lu M, Zhu K. Microarray analysis to identify the similarities and differences of pathogenesis between aortic occlusive disease and abdominal aortic aneurysm. Vascular. 2017;:1708538117736695 pubmed publisher
    ..ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions...
  36. Valenzuela S, Martin D, Por S, Robbins J, Warton K, Bootcov M, et al. Molecular cloning and expression of a chloride ion channel of cell nuclei. J Biol Chem. 1997;272:12575-82 pubmed
    ..Its only known homologue is a bovine chloride ion channel protein (p64) believed to localize to internal organelles. NCC27 therefore represents the first human member of a new class of organellar chloride ion channel proteins. ..
  37. Peretti M, Angelini M, Savalli N, Florio T, Yuspa S, Mazzanti M. Chloride channels in cancer: Focus on chloride intracellular channel 1 and 4 (CLIC1 AND CLIC4) proteins in tumor development and as novel therapeutic targets. Biochim Biophys Acta. 2015;1848:2523-31 pubmed publisher
    ..channels (CLICs), could be a promising class of therapeutic targets for its intrinsic individualities: CLIC1 and CLIC4, in particular, not only are overexpressed in specific tumor types or their corresponding stroma but also change ..
  38. Tang H, Beer L, Tanyi J, Zhang R, Liu Q, Speicher D. Protein isoform-specific validation defines multiple chloride intracellular channel and tropomyosin isoforms as serological biomarkers of ovarian cancer. J Proteomics. 2013;89:165-78 pubmed publisher
    ..In addition to CLIC1 and TPM1, which were the proteins initially discovered in a xenograft mouse model, CLIC4, TPM2, TPM3, and TPM4 were present in ovarian cancer patient sera at significantly elevated levels compared with ..
  39. Choi Y, Oh S, Won M, Choi K, Ko M, Seo D, et al. Targeting ODC1 inhibits tumor growth through reduction of lipid metabolism in human hepatocellular carcinoma. Biochem Biophys Res Commun. 2016;478:1674-81 pubmed publisher
    ..It revealed that 119 genes show same directional regulation (76 up- and 43 down-regulated) in both Huh1 and Huh7 cells and were considered as a common ODC1 knockdown signature...
  40. Haga Y, Kanda T, Nakamoto S, Nakamura M, Sasaki R, Wu S, et al. Interferon induces interleukin 8 and bone marrow stromal cell antigen 2 expression, inhibiting the production of hepatitis B virus surface antigen from human hepatocytes. Biochem Biophys Res Commun. 2017;486:858-863 pubmed publisher
    ..In PLC/PRF/5 and Huh1, IFN?-2a treatment decreased HBsAg levels in their conditioned media...
  41. Spiekerkoetter E, Guignabert C, de Jesus Perez V, Alastalo T, Powers J, Wang L, et al. S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho-extracellular signal-regulated kinase and chloride intracellular channel 4. Circ Res. 2009;105:639-47, 13 p following 647 pubmed publisher
    ..Reduced CLIC4 expression does not interfere with S100A4/Mts1 internalization or its interaction with myosin heavy chain IIA, but ..
  42. Hashimoto N, Hamamura K, Kotani N, Furukawa K, Kaneko K, Honke K, et al. Proteomic analysis of ganglioside-associated membrane molecules: substantial basis for molecular clustering. Proteomics. 2012;12:3154-63 pubmed publisher
    ..Representative examples of GD3-associated molecules such as neogenin and MCAM were shown...
  43. Chuang J, Milner T, Zhu M, Sung C. A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons. J Neurosci. 1999;19:2919-28 pubmed
    ..Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow...
  44. Argenzio E, Margadant C, Leyton Puig D, Janssen H, Jalink K, Sonnenberg A, et al. CLIC4 regulates cell adhesion and β1 integrin trafficking. J Cell Sci. 2014;127:5189-203 pubmed publisher
    b>Chloride intracellular channel protein 4 (CLIC4) exists in both soluble and membrane-associated forms, and is implicated in diverse cellular processes, ranging from ion channel formation to intracellular membrane remodeling...
  45. Okudela K, Katayama A, Woo T, Mitsui H, Suzuki T, Tateishi Y, et al. Proteome analysis for downstream targets of oncogenic KRAS--the potential participation of CLIC4 in carcinogenesis in the lung. PLoS ONE. 2014;9:e87193 pubmed publisher
    ..b>Chloride intracellular channel protein 4 (CLIC4), S100 proteins (S100A2 and S100A11), tropomyosin 2, cathepsin L1, integrins?3, eukaryotic ..
  46. Shukla A, Edwards R, Yang Y, Hahn A, Folkers K, Ding J, et al. CLIC4 regulates TGF-β-dependent myofibroblast differentiation to produce a cancer stroma. Oncogene. 2014;33:842-50 pubmed publisher
    ..b>CLIC4, an integral protein in TGF-β signaling, is highly upregulated in stroma of multiple human cancers, and ..
  47. Tung J, Hobert O, Berryman M, Kitajewski J. Chloride intracellular channel 4 is involved in endothelial proliferation and morphogenesis in vitro. Angiogenesis. 2009;12:209-20 pubmed publisher
    ..Recently, the chloride intracellular channel 4 (CLIC4)-orthologue EXC-4 was found to be necessary for proper development and maintenance of the Caenorhabditis elegans ..
  48. Li N, Li H, Jiang H, Shen L, Yan X, Guo J, et al. Mutation detection in candidate genes for benign familial infantile seizures on a novel locus. Int J Neurosci. 2010;120:217-21 pubmed publisher
    ..The analyses suggest those candidate genes that were detected might not be involved in the epileptogenesis of pure BFIS, at least in the Chinese family we studied. ..
  49. Xu Y, Kang J, Yuan Z, Li H, Su J, Li Y, et al. Suppression of CLIC4/mtCLIC enhances hydrogen peroxide-induced apoptosis in C6 glioma cells. Oncol Rep. 2013;29:1483-91 pubmed publisher
    CLIC4/mtCLIC (referred to here as CLIC4) is one of the seven-member family of chloride intracellular channels (CLIC)...
  50. Edwards J. A novel p64-related Cl- channel: subcellular distribution and nephron segment-specific expression. Am J Physiol. 1999;276:F398-408 pubmed
    ..conclude that this new protein represents the human version of a previously described protein from rat brain named p64H1. The human version of p64H1 (huH1) is a 28...
  51. Singh H, Cousin M, Ashley R. Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin. FEBS J. 2007;274:6306-16 pubmed
    ..In this study, we incorporated purified, recombinant mammalian CLIC1, CLIC4 and (for the first time) CLIC5 into planar lipid bilayers, and tested the hypothesis that the channels are ..
  52. Tasiopoulou V, Magouliotis D, Solenov E, Vavougios G, Molyvdas P, Gourgoulianis K, et al. Transcriptional over-expression of chloride intracellular channels 3 and 4 in malignant pleural mesothelioma. Comput Biol Chem. 2015;59 Pt A:111-6 pubmed publisher
    ..In MPM, the gene expressions of CLIC3 and CLIC4 were significantly increased compared to controls (p=0.001 and p<0.001 respectively)...
  53. Yu Q, Zhou X, Xia Q, Shen J, Yan J, Zhu J, et al. SiRNA-Mediated Down-Regulation of CLIC4 Gene Inhibits Cell Proliferation and Accelerates Cell Apoptosis of Mouse Liver Cancer Hca-F and Hca-P Cells. J Cell Biochem. 2018;119:659-668 pubmed publisher
    This study explored the effects involved in silencing CLIC4 on apoptosis and proliferation of mouse liver cancer Hca-F and Hca-P cells...
  54. Kenny T, Schmidt H, Adelson K, Hoshida Y, Koh A, Shah N, et al. Patient-derived Interstitial Fluids and Predisposition to Aggressive Sporadic Breast Cancer through Collagen Remodeling and Inactivation of p53. Clin Cancer Res. 2017;23:5446-5459 pubmed publisher
    ..found that IFHigh induces myofibroblast differentiation and collagen production through activation of CLIC4. IFHigh also downregulates RYBP, leading to degradation of p53...
  55. Zhong J, Kong X, Zhang H, Yu C, Xu Y, Kang J, et al. Inhibition of CLIC4 enhances autophagy and triggers mitochondrial and ER stress-induced apoptosis in human glioma U251 cells under starvation. PLoS ONE. 2012;7:e39378 pubmed publisher
    CLIC4/mtCLIC, a chloride intracellular channel protein, localizes to mitochondria, endoplasmic reticulum (ER), nucleus and cytoplasm, and participates in the apoptotic response to stress...
  56. Zou Q, Yang Z, Li D, Liu Z, Yuan Y. Association of chloride intracellular channel 4 and Indian hedgehog proteins with survival of patients with pancreatic ductal adenocarcinoma. Int J Exp Pathol. 2016;97:422-429 pubmed publisher
    ..The aim of this study was to examine chloride intracellular channel 4 (CLIC4) and Indian Hedgehog (Ihh) expression in benign and malignant lesions of the pancreas and to examine the eventual ..
  57. Shanks R, Larocca M, Berryman M, Edwards J, Urushidani T, Navarre J, et al. AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member. J Biol Chem. 2002;277:40973-80 pubmed
    ..Furthermore, GFP-CLIC5B-(178-410) targeted to the Golgi apparatus in HCA-7 cells. The results suggest that AKAP350 associates with CLIC proteins and specifically that CLIC5B interacts with AKAP350 at the Golgi apparatus in HCA-7 cells. ..
  58. Wojciak Stothard B, Abdul Salam V, Lao K, Tsang H, Irwin D, Lisk C, et al. Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension. Circulation. 2014;129:1770-80 pubmed publisher
    Chloride intracellular channel 4 (CLIC4) is highly expressed in the endothelium of remodeled pulmonary vessels and plexiform lesions of patients with pulmonary arterial hypertension...
  59. Deng Y, Tang N, Liu C, Zhang J, An S, Peng Y, et al. CLIC4, ERp29, and Smac/DIABLO derived from metastatic cancer stem-like cells stratify prognostic risks of colorectal cancer. Clin Cancer Res. 2014;20:3809-17 pubmed publisher
    ..We identified three proteins, CLIC4, ERp29, and Smac/DIABLO, from metastatic cancer stem-like cells of colorectal cancer and verified the proteins' ..
  60. Kloeker S, Wadzinski B. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J Biol Chem. 1999;274:5339-47 pubmed
    ..These data indicate that one form of PP4 is similar to the core complex of PP2A in that it consists of a catalytic subunit and a "PP2AA-like" structural subunit. ..
  61. Berryman M, Bretscher A. Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. Mol Biol Cell. 2000;11:1509-21 pubmed
    ..We cloned human cDNAs and generated antibodies specific for CLIC5, CLIC1/NCC27, and CLIC4/huH1/p64H1. CLIC5 shares 52-76% overall identity with human CLIC1, CLIC2, CLIC3, and CLIC4...
  62. Wick M, Burger C, Brüsselbach S, Lucibello F, Muller R. Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression. J Cell Sci. 1994;107 ( Pt 1):227-39 pubmed
    ..This supports the hypothesis that the progression into S from either G0 or G1 are distinct processes with specific patterns of gene expression. ..