CLEC9A

Summary

Gene Symbol: CLEC9A
Description: C-type lectin domain containing 9A
Alias: CD370, DNGR-1, DNGR1, UNQ9341, C-type lectin domain family 9 member A, HEEE9341
Species: human
Products:     CLEC9A

Top Publications

  1. Huysamen C, Willment J, Dennehy K, Brown G. CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem. 2008;283:16693-701 pubmed publisher
    We describe here the first characterization of CLEC9A, a group V C-type lectin-like receptor located in the "Dectin-1 cluster" of related receptors, which are encoded within the natural killer (NK)-gene complex...
  2. Sancho D, Mourão Sá D, Joffre O, Schulz O, Rogers N, Pennington D, et al. Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008;118:2098-110 pubmed publisher
    ..We conclude that DNGR-1 is a novel, highly specific marker of mouse and human DC subsets that can be exploited for CTL cross-priming and tumor therapy. ..
  3. Caminschi I, Proietto A, Ahmet F, Kitsoulis S, Shin Teh J, Lo J, et al. The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement. Blood. 2008;112:3264-73 pubmed publisher
    A novel dendritic cell (DC)-restricted molecule, Clec9A, was identified by gene expression profiling of mouse DC subtypes. Based on sequence similarity, a human ortholog was identified...
  4. Harpur C, Kato Y, Dewi S, Stankovic S, Johnson D, Bedoui S, et al. Classical Type 1 Dendritic Cells Dominate Priming of Th1 Responses to Herpes Simplex Virus Type 1 Skin Infection. J Immunol. 2018;: pubmed publisher
    ..inoculation was rescued by targeted Ag delivery to CD8α+ and CD103+ cDC1 using an anti-Clec9A Ab construct...
  5. Cauwels A, Van Lint S, Catteeuw D, Pang S, Paul F, Rogge E, et al. Targeting interferon activity to dendritic cells enables in vivo tolerization and protection against EAE in mice. J Autoimmun. 2018;: pubmed publisher
    ..In contrast, AFN targeted to dendritic cells (DC, via Clec9A) protected without mortality or hematological consequences...
  6. del Fresno C, Saz Leal P, Enamorado M, Wculek S, Martínez Cano S, Blanco Menéndez N, et al. DNGR-1 in dendritic cells limits tissue damage by dampening neutrophil recruitment. Science. 2018;362:351-356 pubmed publisher
    ..cells (cDC1s) express dendritic cell natural killer lectin group receptor-1 (DNGR-1), encoded by the gene Clec9a, which senses tissue damage and favors cross-presentation of dead-cell material to CD8+ T cells...
  7. Belluschi S, Calderbank E, Ciaurro V, Pijuan Sala B, Santoro A, Mende N, et al. Myelo-lymphoid lineage restriction occurs in the human haematopoietic stem cell compartment before lymphoid-primed multipotent progenitors. Nat Commun. 2018;9:4100 pubmed publisher
    ..programmes and lineage potential progressively change along a gradient of opposing cell surface expression of CLEC9A and CD34...
  8. Van Brussel I, Ammi R, Rombouts M, Cools N, Vercauteren S, de Roover D, et al. Fluorescent activated cell sorting: an effective approach to study dendritic cell subsets in human atherosclerotic plaques. J Immunol Methods. 2015;417:76-85 pubmed publisher
    ..We have developed a flow cytometry-based method to characterize and isolate different DC subsets (i.e. CD11b(+), Clec9A(+) and CD16(+) conventional (c)DC and CD123(+) plasmacytoid (p)DC) in human atherosclerotic plaques...
  9. McGovern N, Schlitzer A, Gunawan M, Jardine L, Shin A, Poyner E, et al. Human dermal CD14⁺ cells are a transient population of monocyte-derived macrophages. Immunity. 2014;41:465-477 pubmed publisher
    ..The DC network is evolutionarily conserved; the homologs of human tissue CD141(hi)XCR1⁺ CLEC9A⁺ DCs and CD1c⁺ DCs are murine CD103⁺ DCs and CD64⁻ CD11b⁺ DCs...

More Information

Publications49

  1. Reuter A, Panozza S, Macri C, Dumont C, Li J, Liu H, et al. Criteria for dendritic cell receptor selection for efficient antibody-targeted vaccination. J Immunol. 2015;194:2696-705 pubmed publisher
    ..Next, we analyzed parameters of DEC205 (CD205), Clec9A, CD11c, CD11b, and CD40 endocytosis and obtained quantitative measurements of internalization speed, surface ..
  2. Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
    ..progenitors with greater developmental potential, the hpre-CDC is restricted to producing CD1c(+) and CD141(+) Clec9a(+) cDCs...
  3. Petersen T, Knight D, Tang C, Osmond T, Hermans I. Batf3-independent langerin- CX3CR1- CD8α+ splenic DCs represent a precursor for classical cross-presenting CD8α+ DCs. J Leukoc Biol. 2014;96:1001-10 pubmed publisher
    ..CD8α(+) DC can be divided into langerin-positive and negative populations, both of which express DEC205, Clec9A, and high basal levels of CD86...
  4. van der Aa E, van de Laar L, Janssen H, van Montfoort N, Woltman A. BDCA3 expression is associated with high IFN-λ production by CD34(+)-derived dendritic cells generated in the presence of GM-CSF, IL-4, and/or TGF-β. Eur J Immunol. 2015;45:1471-81 pubmed publisher
    ..together further increased CD14(+)-derived and CD1a(+)-derived BDCA3(+) DC frequencies, which partly expressed CLEC9A, but were not identical to the BDCA3(high) CLEC9A(+) DC subset in vivo...
  5. Li J, Ahmet F, Sullivan L, Brooks A, Kent S, De Rose R, et al. Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates. Eur J Immunol. 2015;45:854-64 pubmed publisher
    ..used in the mouse, to generate strong humoral responses in the absence of adjuvant is to target antigen to Clec9A, also known as DNGR-1, a receptor on CD8α(+) DCs. Here, we address two issues relating to clinical application...
  6. Sluijter B, van den Hout M, Koster B, van Leeuwen P, Schneiders F, van de Ven R, et al. Arming the Melanoma Sentinel Lymph Node through Local Administration of CpG-B and GM-CSF: Recruitment and Activation of BDCA3/CD141(+) Dendritic Cells and Enhanced Cross-Presentation. Cancer Immunol Res. 2015;3:495-505 pubmed publisher
    ..increased frequencies of SLN-resident BDCA3/CD141(+) cDC subsets that also expressed the C-type lectin receptor CLEC9A. Correlative in vivo analyses and in vitro studies provided evidence that these subsets were derived from BDCA3(+) ..
  7. Gilfillan C, Kuhn S, Baey C, Hyde E, Yang J, Ruedl C, et al. Clec9A+ Dendritic Cells Are Not Essential for Antitumor CD8+ T Cell Responses Induced by Poly I:C Immunotherapy. J Immunol. 2018;200:2978-2986 pubmed publisher
    ..We used a murine orthotopic mammary tumor model, as well as Clec9A-diphtheria toxin receptor mice that can be depleted of the specialized cross-presenting CD8α+ and ..
  8. Yao W, Yu L, Li D, Yang G. Molecular cloning and characterization of DNGR-1 in rhesus macaques. Mol Immunol. 2017;87:217-226 pubmed publisher
    DC, NK lectin group receptor-1 (DNGR-1), also known as C-type lectin domain family 9 member A (CLEC9A), is a promising target for immunological therapeutics and vaccination against tumors and viruses...
  9. Macri C, Dumont C, Panozza S, Lahoud M, Caminschi I, Villadangos J, et al. Antibody-mediated targeting of antigen to C-type lectin-like receptors Clec9A and Clec12A elicits different vaccination outcomes. Mol Immunol. 2017;81:143-150 pubmed publisher
    ..C-type lectin-like receptors Clec9A and Clec12A are attractive receptor targets however their targeting in vivo elicits significantly different ..
  10. Hang L, Blum A, Kumar S, Urban J, Mitreva M, Geary T, et al. Downregulation of the Syk Signaling Pathway in Intestinal Dendritic Cells Is Sufficient To Induce Dendritic Cells That Inhibit Colitis. J Immunol. 2016;197:2948-57 pubmed publisher
    ..It was observed that gut DCs express mRNA encoding for C-type lectin (CLEC) 7A, CLEC9A, CLEC12A, and CLEC4N. H. polygyrus bakeri infection downmodulated CLEC mRNA expression in these cells...
  11. Cui T, Maheshwer B, Hong J, Goldsmith A, Bentley J, Popova A. Hyperoxic Exposure of Immature Mice Increases the Inflammatory Response to Subsequent Rhinovirus Infection: Association with Danger Signals. J Immunol. 2016;196:4692-705 pubmed publisher
    ..Hyperoxia also increased expression of Clec9a, a CD103(+) DC-specific damaged cell-recognition molecule...
  12. Böttcher J, Zelenay S, Rogers N, Helft J, Schraml B, Reis E Sousa C. Oncogenic Transformation of Dendritic Cells and Their Precursors Leads to Rapid Cancer Development in Mice. J Immunol. 2015;195:5066-76 pubmed publisher
    ..mouse model in which expression of Cre recombinase driven by the C-type lectin domain family 9, member a (Clec9a) locus causes expression of the Kirsten rat sarcoma viral oncogene homolog (Kras)(G12D) oncogenic driver and ..
  13. Clément M, Haddad Y, Raffort J, Lareyre F, Newland S, Master L, et al. Deletion of IRF8 (Interferon Regulatory Factor 8)-Dependent Dendritic Cells Abrogates Proatherogenic Adaptive Immunity. Circ Res. 2018;122:813-820 pubmed publisher
    ..DCs originating from a DNGR1+ (dendritic cell natural killer lectin group receptor 1) precursor (Clec9a+/creRosa+/EYFP mice), we first show that YFPhiCD11c..
  14. Koh V, Ng S, Ang M, Lin W, Ruedl C, Alonso S. Role and contribution of pulmonary CD103+ dendritic cells in the adaptive immune response to Mycobacterium tuberculosis. Tuberculosis (Edinb). 2017;102:34-46 pubmed publisher
    ..Using CLEC9A-DTR transgenic mice enabling the inducible depletion of CD103+ DCs, we established that this DC subset ..
  15. Yao W, Li D, Yu L, Wang F, Xing H, Yang G. The levels of DNGR-1 and its ligand-bearing cells were altered after human and simian immunodeficiency virus infection. Immunol Res. 2017;65:869-879 pubmed publisher
    Dendritic cell NK lectin Group Receptor-1 (DNGR-1), also known as C-type lectin domain family 9, member A (CLEC9A), is a member of C-type lectin receptor superfamily expressed primarily on dendritic cells (DC) that excel in cross-..
  16. Kato Y, Zaid A, Davey G, Mueller S, Nutt S, Zotos D, et al. Targeting Antigen to Clec9A Primes Follicular Th Cell Memory Responses Capable of Robust Recall. J Immunol. 2015;195:1006-14 pubmed publisher
    ..b>Clec9A (DNGR-1), which is expressed by CD8(+) DCs, has been shown to bind F-actin exposed on damaged cells...
  17. Haddad Y, Lahoute C, Clément M, Laurans L, Metghalchi S, Zeboudj L, et al. The Dendritic Cell Receptor DNGR-1 Promotes the Development of Atherosclerosis in Mice. Circ Res. 2017;121:234-243 pubmed publisher
    ..b>Clec9a (C-type lectin receptor) or DNGR-1 (dendritic cell NK lectin group receptor-1) is preferentially expressed by the ..
  18. Quintana E, Fernández A, Velasco P, de Andrés B, Liste I, Sancho D, et al. DNGR-1(+) dendritic cells are located in meningeal membrane and choroid plexus of the noninjured brain. Glia. 2015;63:2231-48 pubmed publisher
    ..C-type lectin receptor family 9, member A (DNGR-1/CLEC9A) is a marker of specific DC subsets that share functional similarities, such as CD8α(+) DCs in lymphoid tissues ..
  19. Ramos M, Teunissen M, Helder B, Aarrass S, de Hair M, van Kuijk A, et al. Reduced CLEC9A expression in synovial tissue of psoriatic arthritis patients after adalimumab therapy. Rheumatology (Oxford). 2016;55:1575-84 pubmed publisher
    We aimed to investigate the early changes in expression of C-type lectin domain family 9, member A (CLEC9A), a C-type lectin that is specifically expressed by the CD141(+) dendritic cell subset that is involved in cross-presentation to ..
  20. Torres D, Kohler A, Delbauve S, Caminschi I, Lahoud M, Shortman K, et al. IL-12p40/IL-10 Producing preCD8?/Clec9A+ Dendritic Cells Are Induced in Neonates upon Listeria monocytogenes Infection. PLoS Pathog. 2016;12:e1005561 pubmed publisher
    ..We characterized a dominant functional Batf3-dependent precursor of CD11c(high) DCs that is Clec9A+CD205+CD24+ but CD8?- at 3 days of life...
  21. Muzaki A, Tetlak P, Sheng J, Loh S, Setiagani Y, Poidinger M, et al. Intestinal CD103(+)CD11b(-) dendritic cells restrain colitis via IFN-γ-induced anti-inflammatory response in epithelial cells. Mucosal Immunol. 2016;9:336-51 pubmed publisher
    ..We show that Clec9A-diphtheria toxin receptor (DTR) mice after depletion of CD103(+)CD11b(-) DCs developed severe, low-dose dextran ..
  22. Eggink L, Spyroulias G, Jones N, Hanson C, Hoober J. A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D. PLoS ONE. 2015;10:e0130532 pubmed publisher
    ..Binding of svH1C was not detected with CLEC9a, CLEC10a or DC-SIGN, which are lectin-type receptors specific for other sugars...
  23. Lu L, Tanaka Y, Ishii N, Sasano T, Sugawara S. CD103+ CD11b- salivary gland dendritic cells have antigen cross-presenting capacity. Eur J Immunol. 2017;47:305-313 pubmed publisher
    ..CD103+ CD11b- cDCs expressed XCR1, CLEC9A, and interferon regulatory factor 8, whereas CD103- CD11b+ cDCs strongly expressed CD172a...
  24. Kassianos A, Wang X, Sampangi S, MUCZYNSKI K, Healy H, Wilkinson R. Increased tubulointerstitial recruitment of human CD141(hi) CLEC9A(+) and CD1c(+) myeloid dendritic cell subsets in renal fibrosis and chronic kidney disease. Am J Physiol Renal Physiol. 2013;305:F1391-401 pubmed publisher
    ..CD141(hi) DCs expressed C-type lectin domain family 9 member A (CLEC9A), whereas the majority of CD1c(+) DCs lacked the expression of CD1a and DC-specific ICAM-3-grabbing nonintegrin (..
  25. Helft J, Anjos Afonso F, van der Veen A, Chakravarty P, Bonnet D, Reis E Sousa C. Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors. Cell Rep. 2017;20:529-537 pubmed publisher
    ..MLPs) gives rise to cDCs, in particular the subset known as cDC1, identified by co-expression of DNGR-1 (CLEC9A) and CD141 (BDCA-3)...
  26. van der Aa E, van Montfoort N, Woltman A. BDCA3(+)CLEC9A(+) human dendritic cell function and development. Semin Cell Dev Biol. 2015;41:39-48 pubmed publisher
    ..Recently, gene expression profiling identified BDCA3(+)CLEC9A(+) DC as a separate human DC subset...
  27. Ahrens S, Zelenay S, Sancho D, Hanč P, Kjær S, Feest C, et al. F-actin is an evolutionarily conserved damage-associated molecular pattern recognized by DNGR-1, a receptor for dead cells. Immunity. 2012;36:635-45 pubmed publisher
    ..In this regard, we have previously described DNGR-1 (CLEC9A) as a DC-restricted receptor specific for an unidentified DAMP that is exposed by necrotic cells and is necessary ..
  28. Yan Z, Wu Y, Du J, Li G, Wang S, Cao W, et al. A novel peptide targeting Clec9a on dendritic cell for cancer immunotherapy. Oncotarget. 2016;7:40437-40450 pubmed publisher
    Dendritic cells (DCs) are professional antigen-presenting cells with antigen recognition molecules on the surface. Clec9a is selectively expressed on mouse CD8a+ DCs and CD103+ DCs subsets, which are functionally similar to human BDCA3+ ..
  29. Zhang J, Czabotar P, Policheni A, Caminschi I, Wan S, Kitsoulis S, et al. The dendritic cell receptor Clec9A binds damaged cells via exposed actin filaments. Immunity. 2012;36:646-57 pubmed publisher
    ..The damaged cell-recognition molecule Clec9A is expressed on the surface of the mouse and human dendritic cell subsets specialized for the uptake and ..
  30. Ghinnagow R, De Meester J, Cruz L, Aspord C, Corgnac S, Macho Fernandez E, et al. Co-delivery of the NKT agonist ?-galactosylceramide and tumor antigens to cross-priming dendritic cells breaks tolerance to self-antigens and promotes antitumor responses. Oncoimmunology. 2017;6:e1339855 pubmed publisher
    ..To this end, we designed a nanoparticle-based vaccine to target cross-priming DCs via the Clec9a endocytic pathway...
  31. Schreibelt G, Klinkenberg L, Cruz L, Tacken P, Tel J, Kreutz M, et al. The C-type lectin receptor CLEC9A mediates antigen uptake and (cross-)presentation by human blood BDCA3+ myeloid dendritic cells. Blood. 2012;119:2284-92 pubmed publisher
    b>CLEC9A is a recently discovered C-type lectin receptor involved in sensing necrotic cells...
  32. Sancho D, Joffre O, Keller A, Rogers N, Martinez D, Hernanz Falcón P, et al. Identification of a dendritic cell receptor that couples sensing of necrosis to immunity. Nature. 2009;458:899-903 pubmed publisher
    ..Here we show that CD8alpha+ dendritic cells use CLEC9A (also known as DNGR-1), a recently-characterized C-type lectin, to recognize a preformed signal that is exposed on ..
  33. Poulin L, Salio M, Griessinger E, Anjos Afonso F, Craciun L, Chen J, et al. Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells. J Exp Med. 2010;207:1261-71 pubmed publisher
    ..Here, we characterize a population of human DCs that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8alpha+ DCs in phenotype and function...
  34. Cheng A, Yang K, Chen N, Hsu T, Jou R, Hsieh S, et al. CLEC9A modulates macrophage-mediated neutrophil recruitment in response to heat-killed Mycobacterium tuberculosis H37Ra. PLoS ONE. 2017;12:e0186780 pubmed publisher
    ..In this study, based on an in vitro lectin binding assay, CLEC9A (DNGR-1) is identified as a novel CLR that binds with mycobacteria...
  35. Balan S, Dalod M. In Vitro Generation of Human XCR1(+) Dendritic Cells from CD34(+) Hematopoietic Progenitors. Methods Mol Biol. 2016;1423:19-37 pubmed publisher
    ..Human CD141(+)CLEC9A(+)XCR1(+) DCs constitute a very interesting DC subset for the design of immunotherapeutic treatments against ..
  36. Cauwels A, Van Lint S, Paul F, Garcin G, De Koker S, Van Parys A, et al. Delivering Type I Interferon to Dendritic Cells Empowers Tumor Eradication and Immune Combination Treatments. Cancer Res. 2018;78:463-474 pubmed publisher
    ..Type I IFN-derived AcTaferon (AFN)-targeting Clec9A+ dendritic cells (DC) displayed strong antitumor activity in murine melanoma, breast carcinoma, and ..
  37. Phongsisay V, Iizasa E, Hara H, Yoshida H. Pertussis toxin targets the innate immunity through DAP12, FcR?, and MyD88 adaptor proteins. Immunobiology. 2017;222:664-671 pubmed publisher
    ..and activation (MyD88-associated TLR4, DAP12-coupled LMIR5/CD300b, FcR?-coupled LMIR8/CD300c, CLEC9A, MGL-1). DAP12, MyD88, and FcR? were selected for further investigation...
  38. Tetlak P, Ruedl C. Analysis of Dendritic Cell Function Using Clec9A-DTR Transgenic Mice. Methods Mol Biol. 2016;1423:275-89 pubmed publisher
    The Clec9A-diphtheria toxin receptor (DTR) transgenic mouse strain provides a robust animal model to study the function of lymphoid organ-resident CD8(+) dendritic cells (DCs) and nonlymphoid organ-specific CD103(+) DCs in infectioous ..
  39. Poulin L, Reyal Y, Uronen Hansson H, Schraml B, Sancho D, Murphy K, et al. DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues. Blood. 2012;119:6052-62 pubmed publisher
    ..Here we show that mouse CD8?(+) DCs and CD103(+) CD11b(-) DCs can be defined by shared high expression of DNGR-1 (CLEC9A)...
  40. Lahoud M, Ahmet F, Kitsoulis S, Wan S, Vremec D, Lee C, et al. Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype. J Immunol. 2011;187:842-50 pubmed publisher
    ..For the production of cytotoxic T cells, DEC-205 and Clec9A, but not Clec12A, were effective targets, although only in the presence of adjuvants...