CLCA4

..Intracellular signaling responses of macrophages to HIV-1 may modulate postentry steps of infection and cell functions apart from infection...

..injury-induced neuropathic pain in rats by using ivermectin, a ligand gated chloride channel opener and NPPB, a CaCC blocker. The effect of ivermectin (5, 10, 20 mg/kg i.p. or 50, 100 and 200 microg/rat by i.c.v...

..Here we investigated the role of a candidate CaCC protein, bestrophin-2 (Best2), using Best2-/- mice...

..by three independent groups, using different strategies, of TMEM16A as the molecular counterpart of the CaCC is discussed. TMEM16A is part of a family that has 10 other members in mice...

..both a cystic fibrosis transmembrane conductance regulator (CFTR) inhibitor and a Ca(2+)-activated Cl(-) channel (CaCC) inhibitor. HDM extract also stimulated fluid secretion from porcine airway mucosa...

..The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C...

..Potent, selective CaCC inhibitors have not been available...

..585+/-0.01 fmol/microl), low 5-HT (0.334+/-0.032 fmol/microl) and high DOPAC/DA ratio (265.11+/-20.73) in intact cACC. 6-OHDA alone (8 microg/2 microl) depleted DA (-66%), DOPAC (-65%), and NE (-62%)...

..Our objective was to develop clinical practice guidelines for the diagnosis and treatment of patients with primary aldosteronism...

..and fixed-bed column studies were undertaken to evaluate the performance of clay-active coal-coated cement (CACC) in removing phenols from aqueous solution...

TMEM16A was found recently to be a calcium-activated Cl(-) channel (CaCC)...

..We found a TATA-like sequence, a GC box, four CACC boxes, four GATA consensus sequences, and six leader-binding protein-1 binding motifs in the promoter region of the ..

..on chromosome 16 in a mouse erythroleukemia cell environment revealed protein occupancy at GATA-1, AP-1/NF-E2, and CACC/GGTGG motifs, specific differences compared with in vitro protein binding, and distinct changes in one region upon ..

..1 inhibited calcium-activated Cl(-) currents (I(ClCa)) and if the pharmacological overlap between K(Ca)1.1 and CaCCs extends to intermediate and small conductance, calcium-activated K(+) channels...

Does inositol 3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P(4)) inhibit apical Ca(2+)-activated Cl(-) conductance (CaCC)? We studied this question using human CFPAC-1 pancreatoma cells grown in polarized monolayers...

..Ca(2+)-activated chloride conductance is confirmed and the putative Ca(2+)-activated chloride channel (CaCC) is further characterized in term of its pharmacological sensitivity...

..In analysing the putative binding elements within the proximal promoter, a CACC-box sequence overlapping a consensus Sp1 element has been identified...

..and extracellular amines concentration by 6-hydroxydopamine (6-OHDA)-induced lesion of core nucleus accumbens (cACC) of rats...

..several sequences matching transcription factor binding sites, including nine SP-1 sites, one AP-1 site, and three CACC boxes...

..To observe the dynamic responses of the cortical areas related to the pain processing by using the differential regression analysis (DRA) technique in functional magnetic resonance imaging (fMRI) and investigation of pain mechanisms...

Activation of an apical Ca(2+)-dependent Cl(-) channel (CaCC) is the rate-limiting step for fluid secretion in many exocrine tissues...

..characterized by a highly conserved domain which is formed by three Zn fingers, which bind to the GC box or the GT/CACC box in the promoter of many genes...

..CaCCs) play a role in airway epithelial Cl(-) secretion, but until recently, the molecular identity of the airway CaCC(s) was unknown. Recent studies have unequivocally identified TMEM16A as a glandular epithelial CaCC...

..Several conductances, such as Ca(2+)-activated Cl() channels (CaCC) and non-selective cation channels (NSCC) have been suggested to be involved in slow wave depolarization...

..The inhibitor of adenylate cyclase, MDL12330A (20 microM), and Ca(2+)-dependent Cl(-) channel (CaCC) blocker, DIDS (100 microM) also significantly reduced the basal current in AT-CF...

..in regulation of uterine contractility, we cloned a novel calcium-activated chloride channel gene, named rat Clca4, from pregnant rat uterus...

Computer-aided clinical cases (CACC) have the potential to complement and/or supplement other types of problem-based learning exercises in modern medical curricula...

..developed a screening assay and screened a library for compounds that could enhance cytoplasmic Ca2+, activate the CaCC, and increase Cl(-) secretion...

..These include CLCA4 and SLC26A2, both implicated in ion transport...

..data, RT-PCR showed that HaCaT keratinocytes express two types of Ca2+-activated Cl- channels, CaCC2 and CaCC3 (CLCA2), as well as the Ca2+-activated K+ channel hSK4...

..We found and validated novel intronic enhancers in both the E2f2 and E2f4 genes, which contain conserved CACC, GATA, and E-BOX elements. The E2f2 enhancer was occupied by EKLF in vivo...

..These features, as well as the presence of several consensus elements such as GATA, LBP-1, E-box, CACC box, and T-antigen motif, indicate that the human AE2 promoter resembles the erythroid promoter of the human AE1 ..

..acid, whereas it was only partially inhibited by CFTR [CFTR(inh)-172, glibenclamide]- and CaCC (niflumic acid)-specific Cl(-) channel blockers...

..inhibitor indomethacin and largely blocked (>80%) by niflumic acid, a calcium-activated chloride channels' (CaCC) blocker. Allergic mice also showed an enhanced response to ATP and thapsigargin...

..The Ca(2+)-activated Cl(-) channel (CaCC), recently identified as TMEM16A, plays important roles in pulmonary vascular function...

..Here, we show that the recently identified calcium-activated chloride channel (CaCC) TMEM16A is expressed in the adult airway surface epithelium and ASM...

..The aim of this study was to determine whether Ca(2+)-activated Cl(-) channels (CaCCs) are involved in central antinociception induced by the activation of µ-, δ- and κ-opioid receptors...

..A close paralogue of TMEM16A, TMEM16B (anoctamin-2), is also associated with CaCC activity, although with different properties...

..to CF can be considered is to recruit alternative chloride channels, such as calcium-activated chloride channel (CaCC), to act as surrogates for CFTR...

Head and neck squamous cell carcinoma (HNSCC) is associated with poor survival. To identify prognostic and diagnostic markers and therapeutic targets, we studied ANO1, a recently identified calcium-activated chloride channel (CaCC).

..that other factors modulate the phenotype including "alternative" calcium-activated anion currents (CaCC)...

..Thus, EKLF is thought to be a stage-specific factor acting through the CACC box in the beta-gene promoter, even though it is already present in embryonic red cells...

The bovine CaCC protein is a putative Ca2+-dependent Cl- channel of airway epithelial cells. Therefore, CaCC proteins could contribute to transepithelial Cl- transport and accordingly modify the phenotype of cystic fibrosis (CF) patients...

..mice requires the activation of Ca(2+)/calmodulin-dependent kinase II (CaMKII) and Ca(2+)-activated Cl(-) channels (CaCC) because simultaneous addition of myristoylated-autocamtide-2-related inhibitory peptide or niflumic acid and CFTR(..

..in primary cultures of rat PASMCs, with small interfering RNAs, was accompanied by almost total loss of whole-cell CaCC currents...

..Previous studies have shown that a CACC cis-regulatory element is important for transcriptional activation in pancreatic insulinoma cells...

..Channel transcripts expressed include ClC-2, Slc26a7 and ClC-Ka, but not Cftr, ClC-1, ClCa1, ClCa2, ClCa3, ClCa4, Slc26a9, ClC-Kb, Best1, Best2, Best3 or the beta-subunit of ClC-K, barttin...

..within +1 to +100 of each of the CYP17 alleles formed complex with the Sp-1 or enhanced binding to the polymorphic CACC box. Genotyping of 510 breast cancer patients and 201 controls revealed no difference in genotype frequencies...

Calcium-activated chloride channels (CaCC) with similar hallmark features are present in many cell types and mediate important physiological functions including epithelial secretion, sensory signal transduction, and smooth muscle ..

One of the most common regulatory elements is the GC box and the related GT/CACC box, which are widely distributed in promoters, enhancers and locus control regions of housekeeping as well as tissue-specific genes...

..As in the human, the mouse Lu gene is organized in 15 exons. The proximal promoter showed consensus CACC-binding sites whereas the distal promoter exhibits a GATA-1-binding site and multiple E boxes...

..students' application of osteopathic principles and practice, a Web-based computer-assisted clinical case (CACC) was designed, for which students were required to submit a SOAP (Subjective, Objective, Assessment, Plan) note...

..We found increased caudal anterior cingulate cortex (cACC) activity during the ongoing task with both implicit target and action cues, suggesting that alertness or ..

..importance of cystic fibrosis transmembrane conductance regulator (CFTR) and calcium-activated chloride channels (CaCC) in Cl secretion by mucous and serous cells of human airway glands...

..RT-PCR experiments did not provide any evidence that the endothelial Ca(2+)-activated Cl- channel might be identical with a recently cloned Ca(2+)-sensitive Cl- channel (CaCC).

..DNA sequence analyses revealed that the 441-base pair promoter contains two CArG/SRF boxes, a CACC box, and one potential MEF-2 binding site, cis-acting elements which are each important regulators of striated ..

..However, three of four CACC boxes and one GATA-1 binding site, identified in the human alpha-LCR, are not conserved in the mouse...

..relationship of the cystic fibrosis transmembrane conductance regulator (CFTR) and Ca(2+) activated Cl(-) channels (CaCC)...

..Double-stranded oligonucleotides containing either the CACC box or the Sp1 consensus sequence efficiently competed with the CCTCCC elements for binding the nuclear extracts...

..The 5'UT region (nucleotides -289 to +9) includes potential binding sites for various transcription factors (Ets, CACC, SP1, GATA-1, AP2) and exhibited a significant transcriptional activity after transfection in the erythroleukemic ..

TMEM16A (ANO1) is a calcium-activated chloride channel (CaCC) expressed in secretory epithelia, smooth muscle, and other tissues...

..of exon 21 of ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) gene responsible for ARHR2 (IVS21+1_3(GTA>CACC))...

Endogenous Ca(2+)-activated Cl(-) channels (CaCC) demonstrate biophysical and pharmacological properties that are well represented in cells overexpressing anoctamin 1 (Ano 1, TMEM16A), a protein that has been identified recently as CaCC...

Complex adaptive chronic care (CACC) is a framework based upon complex adaptive systems' theory developed to address different stages in the patient journey in chronic illness...

..transmembrane 16A (TMEM16A) was demonstrated by three independent research groups to be a strong candidate for the CaCC molecular basis...

..A pharmacological characterization of those two I(sc) components showed the involvement of CaCC and CFTR channel activity in I(fast) and I(late) respectively...

..It has been proposed that duramycin may stimulate chloride secretion through Ca²(+) -activated Cl⁻ channels (CaCC)...

TMEM16A (ANO1) functions as a calcium-activated chloride channel (CaCC). We developed pharmacological tools to investigate the contribution of TMEM16A to CaCC conductance in human airway and intestinal epithelial cells...

We investigated the molecular determinants of Ca(2+)-activated chloride current (CaCC) expressed in adult sensory neurons after a nerve injury...

..is blocked by micromolar H2-DIDS or DTT, reminiscent of a family of epithelial Ca(2+)-activated Cl -channels (CaCC)...

..However, the molecular identity of the olfactory calcium-activated chloride channel (CaCC) is unknown...

..In addition, however, we show that a C/G-rich CACC box in the Po promoter is required for activation by IL6RIL6, as well as by ectopic Sox10, and identify a Kruppel-..

Pancreatic duct cells express a Ca2+-activated Cl- conductance (CaCC), upregulation of which may be beneficial to patients with cystic fibrosis...

..BTEB regulates transcription through its carboxyl-terminal C2H2 zinc finger motif binding to GC or GT/CACC box, a sort of cis-acting elements, which is wildly distributed in promoters, enhancers and control regions of many ..

..factor (EKLF, KLF1) is an essential erythroid cell specific C(2)H(2) zinc finger transcription factor that binds CACC box elements in promoters and distant regulatory elements to activate transcription...

..The present study analyzed vascular endothelial growth factor (VEGF) gene polymorphisms and their impact on the prognosis for patients with gastric cancer...

..Complement assisted cellular cytotoxicity (CACC) was not detectable, although the iC3b ligand and CR3 receptor were present on respectively target and effector ..

..Our aim was to determine the degree of surgical trauma caused by CEA and to define differences related to the use of locoregional or general anesthesia...

..Transmission disequilibrium and the significance of the association decreased within the locus from hCLCA2 towards hCLCA4. Expression of hCLCA1 and hCLCA4 in human rectal mucosa was proven by microarray analysis...

..VRAC), (ii) after an increase in the free intracellular Ca2+ concentration (i.e., the Ca2+-activated Cl- current, CaCC), and (iii) under steady-state isotonic conditions...

..Furthermore, genes that encode enzymes controlling Ins(3,4,5,6)P4 metabolism should be viewed as impacting upon CaCC activity; this, in turn, may influence the severity of the CF condition...

..Lesion studies and functional imaging implicate the anterior cingulate cortex (ACC). The caudal ACC (cACC) has been implicated in cognitive processes such as attention, salience, interference, and response competition, ..

..OH, for which there is no natural intra- or extracellular scavenger, on a Ca(2+)-activated chloride channel (CACC) that participates in Cl(-) secretion in the apical membrane of airway epithelial cells...

..Cl(-) channel (CaCC1, -2, -3) mRNAs are expressed in normal nasal mucosa and nasal polyp, suggesting that altered expression of all CaCC mRNAs in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells.

..Our objective was to develop clinical practice guidelines for the evaluation and treatment of hirsutism in premenopausal women...

..CFTR) and a 4, 4'-diisothiocyano-2, 2'-disulfonic acid stilbene (DIDS)-sensitive Ca(2+)-activated Cl(-) channel (CaCC)...

Human airway epithelia express Ca2+-activated Cl- channels (CaCC) that are activated by extracellular nucleotides (ATP and UTP). CaCC is preserved and seems to be up-regulated in the airways of cystic fibrosis (CF) patients...

..motif discovery tools revealed that genes in the contractile module were found to contain a TATA-box followed by a CACC-box, and are depleted in other GC-rich motifs; whereas genes in the translation module contain a pyrimidine-rich ..

..Thus, the purported CaCC function of the bestrophins and how loss of this function relates to clinical disease needs to be reassessed...

1. A protein isolated from the bovine tracheal epithelium behaves as a Ca2+-activated Cl- channel (CaCC) when incorporated into planar lipid bilayers. 2...

..The molecular identity of the CaCC in PDECs remains unknown...

..Three sites within the proximal promoter (myocyte enhancer factor-2 (MEF2), E-box, and CACC box) were individually mutated, and the effect on luciferase expression was determined...

..Although a number of proteins have been proposed to be responsible for CaCC currents, the anoctamin family (ANO, also known as TMEM16) exhibits characteristics most similar to those expected ..

..to a protein previously isolated from bovine tracheal epithelium that acts as a Ca(2+)-sensitive Cl(-) channel (CaCC) when heterologously expressed or when reconstituted into planar lipid bilayers...

..roles in airway hydration: (1) CFTR, which is cAMP regulated and (2) the Ca(2+)-activated Cl(-) conductance (CaCC) whose molecular identity is uncertain...

..EC50=831 nM) a DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) sensitive and Ca2+ dependent Cl- channel (CaCC). Guanabenz activated a linear Cl- channel with unitary single-channel conductance of 8 pS...

..line) an endogenous calcium- and voltage-dependent, outwardly rectifying small-conductance chloride channel (CACC), and we examined the effects of EGF on CACC activity...

..Mutagenesis of the TATA, CACC, and AT1 sequences of the mouse to those of the cow significantly decreased activity of the mouse myostatin ..

..conductance, either the cystic fibrosis transmembrane conductance regulator (CFTR) or a Ca2+-activated Cl- channel (CaCC)...

..Defolliculated Xenopus oocytes express a robust CaCC formed by a heterologous group of proteins including transmembrane protein 16A (TMEM16A) and bestrophins...

..Recently the increased expression of the human calcium-activated chloride channel 1 (CaCC(1)) was found to play an important role in mucus overproduction in the asthmatic airways...

..The rationale for this approach rests upon the observation that Ca2+-activated C1-conductance (CACC) is preserved in human CF airway epithelia and that organ-specific disease severity in the CF knockout mouse is ..

..the transcription factor Sox-2; an octamer binding motif, which binds the transcription factor Oct-3; and a GT/CACC box, which binds the transcription factors Spi and Sp3...

..examined: 1) dorsolateral prefrontal cortex (DLPFC); 2) Rostral; and 3) Caudal anterior cingulate cortex (rACC and cACC); as well as 4) a control region the primary visual cortex (PVC)...

..be examined: 1) dorsolateral prefrontal cortex (DLPFC);2) Rostral;and 3) Caudal anterior cingulate cortex (rACC and cACC);as well as 4) a control region the primary visual cortex (PVC)...

..Further analyses have identified that the CACC and MEF2 sites within this 270 bp region cooperate in regulating transcription in response to slow innervation...

..via MRS within Dorsolateral Prefrontal White Matter [WM], Rostral and Caudal Anterior Cingulate Cortex [rACC and cACC] as well as a control region the mesial occipital cortex...

..These observations will provide important information regarding the physiological or pathophysiological circumstances under which AVP would contribute to salt retention. ..

..The new technology will help to understand the mechanisms of initiation of such sources, which is the key to developing effective anti-arrhythmic therapies and prevention of sudden cardiac death. ..

..This project may also contribute to the understanding and treatment of motor system disorders as well as demonstrate how the CNS may control non-muscular motor behaviors. ..

..This led to the hypothesis that Best1 is a Ca2+ activated Cl- channel (CaCC) that generates the LP, and that BVMD results from loss of Best1 CaCC activity...

..One form of basal deposit, basal linear deposits, are more common in AMD eyes than age-matched controls. A positive outcome to this study would warrant future clinical trials. ..

..Finally, subjects will be genotyped for known functional MMP promoter polymorphisms, which will be correlated with MMP levels and muscle damage and soreness following eccentric exercise. ..

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..In the third specific aim we will use a novel human RPE culture system to perform studies in Ussing chambers to assess the cellular consequences of bestrophin dysfunction. ..

..This aim will establish the role of CaC channels and bestrophins in kidney physiology and disease. ..

..Specialized support facilities are provided by the Cornell Biotechnology Program, computer facilities, center for research animal resources (including mouse transgenic facilities), and library system. ..

..Successful completion of this project will break ground for a new technology, the 3D imaging of electrical activation in the heart. ..

..The uncovering of these mechanisms may allow us to eventually design and develop new approaches for interfering with this process and specifically targeting agents to organ sites at predictable risk for metastatic involvement...

..abstract_text> ..

..in the roles of chloride in membrane trafficking, neuroscientists interested in the signaling of chloride channels, transport physiologists involved in ion homeostasis, and geneticists interested in channelopathies ..

..The ultimate goal of these studies is to exploit our understanding of the molecular mechanisms of tumor cell/endothelial cell interactions for the development of new anti-metastatic treatment strategies...

..g., anti-Lu-ECAM-1 monoclonal or polyclonal antibodies;synthetic peptides [or carbohydrates] directed against the binding domains; Lu- ECAM-1 anti-sense oligonucleotides). ..

..Understanding the molecular principles that govern extravasation will ultimately allow us to develop strategies in anti-metastatic therapies. ..