Gene Symbol: CLASP2
Description: cytoplasmic linker associated protein 2
Alias: CLIP-associating protein 2, CLIP-associating protein CLASP2, multiple asters (Mast)-like homolog 2, protein Orbit homolog 2
Species: human
Products:     CLASP2

Top Publications

  1. Galjart N. CLIPs and CLASPs and cellular dynamics. Nat Rev Mol Cell Biol. 2005;6:487-98 pubmed
    ..CLIPs and CLASPs interact and cooperate to direct the microtubule network, thereby regulating cellular asymmetry. ..
  2. Nakaya Y, Sukowati E, Sheng G. Epiblast integrity requires CLASP and Dystroglycan-mediated microtubule anchoring to the basal cortex. J Cell Biol. 2013;202:637-51 pubmed publisher
    ..We propose that epiblast-BM interaction requires CLASP- and Dystroglycan-mediated cortical microtubule anchoring, the disruption of which initiates gastrulation EMT. ..
  3. Miller P, Folkmann A, Maia A, Efimova N, Efimov A, Kaverina I. Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells. Nat Cell Biol. 2009;11:1069-80 pubmed publisher
  4. Kumar P, Lyle K, Gierke S, Matov A, Danuser G, Wittmann T. GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009;184:895-908 pubmed publisher
    ..GSK3beta-induced lamella MT destabilization was partially rescued by expression of CLASP2 with mutated phosphorylation sites...
  5. Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller P, Andreyeva N, et al. Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Dev Cell. 2007;12:917-30 pubmed
    ..We propose that Golgi-emanating microtubules contribute to the asymmetric microtubule networks in polarized cells and support diverse processes including post-Golgi transport to the cell front. ..
  6. Akhmanova A, Hoogenraad C, Drabek K, Stepanova T, Dortland B, Verkerk T, et al. Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts. Cell. 2001;104:923-35 pubmed
    ..Antibody injections suggest that CLASP2 is required for the orientation of stabilized microtubules toward the leading edge...
  7. Kumar P, Chimenti M, Pemble H, Schönichen A, Thompson O, Jacobson M, et al. Multisite phosphorylation disrupts arginine-glutamate salt bridge networks required for binding of cytoplasmic linker-associated protein 2 (CLASP2) to end-binding protein 1 (EB1). J Biol Chem. 2012;287:17050-64 pubmed publisher
    ..Here, we use cytoplasmic linker-associated protein 2 (CLASP2) binding to EB1 to determine how multisite phosphorylation regulates interactions with EB1...
  8. Logarinho E, Maffini S, Barisic M, Marques A, Toso A, Meraldi P, et al. CLASPs prevent irreversible multipolarity by ensuring spindle-pole resistance to traction forces during chromosome alignment. Nat Cell Biol. 2012;14:295-303 pubmed publisher
    ..We propose that CLASPs and ninein confer spindle-pole resistance to traction forces exerted during chromosome congression, thereby preventing irreversible spindle multipolarity and aneuploidy. ..
  9. Drabek K, van Ham M, Stepanova T, Draegestein K, van Horssen R, Sayas C, et al. Role of CLASP2 in microtubule stabilization and the regulation of persistent motility. Curr Biol. 2006;16:2259-64 pubmed
    ..By using mouse embryonic fibroblasts (MEFs) in a wound-healing assay, we show here that CLASP2 is required for the formation of a stable, polarized MT array but that CLIP-170 and an APC-EB1 interaction are not ..

More Information


  1. Wittmann T, Waterman Storer C. Spatial regulation of CLASP affinity for microtubules by Rac1 and GSK3beta in migrating epithelial cells. J Cell Biol. 2005;169:929-39 pubmed
  2. Mimori Kiyosue Y, Grigoriev I, Lansbergen G, Sasaki H, Matsui C, Severin F, et al. CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. J Cell Biol. 2005;168:141-53 pubmed
    CLIP-associating protein (CLASP) 1 and CLASP2 are mammalian microtubule (MT) plus-end binding proteins, which associate with CLIP-170 and CLIP-115...
  3. Lee H, Engel U, Rusch J, Scherrer S, Sheard K, Van Vactor D. The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance. Neuron. 2004;42:913-26 pubmed
    ..These and other data suggest a model where Abl acts as a central signaling node to coordinate actin and microtubule dynamics downstream of guidance receptors. ..
  4. Lansbergen G, Grigoriev I, Mimori Kiyosue Y, Ohtsuka T, Higa S, Kitajima I, et al. CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta. Dev Cell. 2006;11:21-32 pubmed
    ..We propose that LL5beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends. ..
  5. Mimori Kiyosue Y, Grigoriev I, Sasaki H, Matsui C, Akhmanova A, Tsukita S, et al. Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment. Genes Cells. 2006;11:845-57 pubmed
    CLASP1 and CLASP2 are homologous mammalian proteins, which associate with the ends of growing microtubules, as well as the cell cortex and the kinetochores of mitotic chromosomes...
  6. Lemos C, Sampaio P, Maiato H, Costa M, Omel yanchuk L, Liberal V, et al. Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization. EMBO J. 2000;19:3668-82 pubmed
    ..The defects observed in the mutant alleles and the intracellular localization of the protein suggest that Mast plays an essential role in centrosome separation and organization of the bipolar mitotic spindle. ..
  7. Pereira A, Pereira A, Maia A, Drabek K, Sayas C, Hergert P, et al. Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function. Mol Biol Cell. 2006;17:4526-42 pubmed
    ..however, only CLASP1 has been directly implicated in cell division, despite the existence of a second paralogue, CLASP2, whose mitotic roles remain unknown...
  8. Maiato H, Fairley E, Rieder C, Swedlow J, Sunkel C, Earnshaw W. Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics. Cell. 2003;113:891-904 pubmed
    ..Our data suggest that CLASP1 is required at kinetochores for attached microtubules to exhibit normal dynamic behavior. ..
  9. Karki P, Ke Y, Tian Y, Ohmura T, Sitikov A, Sarich N, et al. Staphylococcus aureus-induced endothelial permeability and inflammation are mediated by microtubule destabilization. J Biol Chem. 2019;294:3369-3384 pubmed publisher
    ..EC permeability was associated with impaired MT-mediated delivery of cytoplasmic linker-associated protein 2 (CLASP2) to the cell periphery, limiting its interaction with adherens junction proteins...
  10. Hayashi S, Yano M, Igarashi M, Okano H, Okano H. Alternative role of HuD splicing variants in neuronal differentiation. J Neurosci Res. 2015;93:399-409 pubmed publisher
    ..By contrast, HuD-sv4 showed stronger pre-mRNA splicing activity than did HuD-sv1 on Clasp2, which participates in cell division...
  11. Moore C, Zernicka Goetz M. PAR-1 and the microtubule-associated proteins CLASP2 and dynactin-p50 have specific localisation on mouse meiotic and first mitotic spindles. Reproduction. 2005;130:311-20 pubmed
    ..In addition, the microtubule-associated proteins CLASP2 (a CLIP associating protein) and dynactin-p50 are found on kinetochores and a subset of microtubule-organising ..
  12. Siino V, Amato A, Di Salvo F, Caldara G, Filogamo M, James P, et al. Impact of diet-induced obesity on the mouse brain phosphoproteome. J Nutr Biochem. 2018;58:102-109 pubmed publisher
    ..and PPP1R9B), in vesicle trafficking (for example SNAP91 and AMPH) and in cytoskeletal functions (for example, CLASP2 and GSK3B) was identified, while increased phosphorylation was detected for microtubule proteins (such as MAP2 and ..
  13. Liu J, Zhu B, Zhang G, Wang J, Tian W, Ju G, et al. Electric signals regulate directional migration of ventral midbrain derived dopaminergic neural progenitor cells via Wnt/GSK3β signaling. Exp Neurol. 2015;263:113-21 pubmed publisher
    ..of NPCs(vm), which is associated with the down-regulation of GSK3β phosphorylation, β-catenin activation and CLASP2 expression...
  14. Dillon G, Tyler W, Omuro K, Kambouris J, Tyminski C, Henry S, et al. CLASP2 Links Reelin to the Cytoskeleton during Neocortical Development. Neuron. 2017;93:1344-1358.e5 pubmed publisher
    ..However, little is known about how Reelin controls the cytoskeleton during neuronal migration. Here, we identify CLASP2 as a key cytoskeletal effector in the Reelin signaling pathway...
  15. Young S, Besson S, Welburn J. Length-dependent anisotropic scaling of spindle shape. Biol Open. 2014;3:1217-23 pubmed publisher
    ..We find that, in absence of Clasp1 and Clasp2, spindle assembly is biphasic due to unopposed inward pulling forces from the kinetochore-fibers and that ..
  16. Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodríguez García R, et al. CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. Dev Cell. 2018;46:40-58.e8 pubmed publisher
    ..These functions are inhibited by the C-terminal domain of CLASP2, while the TOG1 domain can release this auto-inhibition...
  17. Lawrence E, ArpaÄŸ G, Norris S, Zanic M. Human CLASP2 specifically regulates microtubule catastrophe and rescue. Mol Biol Cell. 2018;: pubmed publisher
    ..components and Total Internal Reflection Fluorescence (TIRF) microscopy to investigate the effects of human CLASP2 on microtubule dynamics in vitro We demonstrate that CLASP2 suppresses microtubule catastrophe and promotes ..
  18. Basu S, Sladecek S, Martinez de la Peña y Valenzuela I, Akaaboune M, Smal I, Martin K, et al. CLASP2-dependent microtubule capture at the neuromuscular junction membrane requires LL5β and actin for focal delivery of acetylcholine receptor vesicles. Mol Biol Cell. 2015;26:938-51 pubmed publisher
    ..We previously showed that microtubule (MT) capture by the plus end-tracking protein CLASP2 regulates AChR density at agrin-induced AChR clusters in cultured myotubes via PI3 kinase acting through GSK3β...
  19. Efimova N, Grimaldi A, Bachmann A, Frye K, Zhu X, Feoktistov A, et al. Podosome-regulating kinesin KIF1C translocates to the cell periphery in a CLASP-dependent manner. J Cell Sci. 2014;127:5179-88 pubmed publisher
    ..Moreover, chimeric mitochondrially targeted CLASP2 recruits KIF1C, suggesting a transient CLASP-KIF1C association...
  20. Beffert U, Dillon G, Sullivan J, Stuart C, Gilbert J, Kambouris J, et al. Microtubule plus-end tracking protein CLASP2 regulates neuronal polarity and synaptic function. J Neurosci. 2012;32:13906-16 pubmed publisher
    ..Here, we present evidence that the microtubule plus-end tracking protein CLASP2 (cytoplasmic linker associated protein 2) is a key regulator of axon and dendrite outgrowth that leads to ..
  21. Zaoui K, Benseddik K, Daou P, Salaün D, Badache A. ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells. Proc Natl Acad Sci U S A. 2010;107:18517-22 pubmed publisher
    ..of GSK3 allows the relocalization of APC (adenomatous polyposis coli) and cytoplasmic linker-associated protein 2 (CLASP2), known MT-associated proteins, to the plasma membrane and ruffles...
  22. Marangi G, Orteschi D, Milano V, Mancano G, Zollino M. Interstitial deletion of 3p22.3p22.2 encompassing ARPP21 and CLASP2 is a potential pathogenic factor for a syndromic form of intellectual disability: a co-morbidity model with additional copy number variations in a large family. Am J Med Genet A. 2013;161A:2890-3 pubmed publisher
    ..Among the transcribed genes residing in this interval, ARPP21 and CLASP2 are proposed as good candidate genes on the basis of their functional properties...
  23. Inkster B, Nichols T, Saemann P, Auer D, Holsboer F, Muglia P, et al. Pathway-based approaches to imaging genetics association studies: Wnt signaling, GSK3beta substrates and major depression. Neuroimage. 2010;53:908-17 pubmed publisher
    ..Our observations lend support to the hypothesis that polymorphisms in GSK3beta play a role in MDD susceptibility or expression, in part, by acting via the canonical Wnt signaling pathway and related substrates. ..
  24. Liu Q, Remmelzwaal S, Heck A, Akhmanova A, Liu F. Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry. Sci Rep. 2017;7:13453 pubmed publisher
    ..the complex formed by MICAL3, ELKS and Rab8A, which is involved in exocytosis, and the complex of SLAIN2, CLASP2 and ch-TOG, which controls microtubule dynamics...
  25. Stehbens S, Paszek M, Pemble H, Ettinger A, Gierke S, Wittmann T. CLASPs link focal-adhesion-associated microtubule capture to localized exocytosis and adhesion site turnover. Nat Cell Biol. 2014;16:561-73 pubmed publisher
    ..We report that clustering of microtubule-associated CLASP1 and CLASP2 proteins around FAs temporally correlates with FA turnover...
  26. Ruiz Sáenz A, van Haren J, Sayas C, Rangel L, Demmers J, Millan J, et al. Protein 4.1R binds to CLASP2 and regulates dynamics, organization and attachment of microtubules to the cell cortex. J Cell Sci. 2013;126:4589-601 pubmed publisher
    ..Cortical platforms, formed by a subset of MT plus-end-tracking proteins, such as CLASP2, and non-MT binding proteins such as LL5?, attach distal ends of MTs to the cell cortex...
  27. Maia A, García Z, Kabeche L, Barisic M, Maffini S, Macedo Ribeiro S, et al. Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore-microtubule attachments. J Cell Biol. 2012;199:285-301 pubmed publisher
    ..In this study, we show that during mitosis the MT- and KT-associated protein CLASP2 is progressively and distinctively phosphorylated by Cdk1 and Plk1 kinases, concomitant with the establishment of ..
  28. Maki T, Grimaldi A, Fuchigami S, Kaverina I, Hayashi I. CLASP2 Has Two Distinct TOG Domains That Contribute Differently to Microtubule Dynamics. J Mol Biol. 2015;427:2379-95 pubmed publisher
    ..Here, we report the crystal structures of the two TOG domains of CLASP2. Both domains consist of six HEAT repeats, which are similar to the canonical paddle-like tubulin-binding TOG ..
  29. Ikeda M, Tanaka K. Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis. Sci Rep. 2017;7:8794 pubmed publisher
    ..attachments, is also involved in the maintenance of SAC activity by binding to Bub1, but not by binding to CLASP2 or CLIP-170...
  30. Shahbazi M, Peña Jimenez D, Antonucci F, Drosten M, Perez Moreno M. Clasp2 ensures mitotic fidelity and prevents differentiation of epidermal keratinocytes. J Cell Sci. 2017;130:683-688 pubmed publisher
    ..keratinocytes, here we report that the expression of the mammalian microtubule and kinetochore-associated protein Clasp2 is intimately associated with the basal proliferative makeup of keratinocytes, and its deficiency leads to ..
  31. Honnappa S, Gouveia S, Weisbrich A, Damberger F, Bhavesh N, Jawhari H, et al. An EB1-binding motif acts as a microtubule tip localization signal. Cell. 2009;138:366-76 pubmed publisher
    ..Our findings establish a general "microtubule tip localization signal" (MtLS) and delineate a unifying mechanism for this subcellular protein targeting process. ..
  32. Basu S, Sladecek S, Pemble H, Wittmann T, Slotman J, van Cappellen W, et al. Acetylcholine receptor (AChR) clustering is regulated both by glycogen synthase kinase 3β (GSK3β)-dependent phosphorylation and the level of CLIP-associated protein 2 (CLASP2) mediating the capture of microtubule plus-ends. J Biol Chem. 2014;289:30857-67 pubmed publisher
    ..We have previously shown that microtubule (MT) capture by CLASP2, a MT plus-end-tracking protein (+TIP), increases the size and receptor density of AChR clusters at the NMJ ..
  33. Lanza D, Meirelles G, Alborghetti M, Abrile C, Lenz G, Kobarg J. FEZ1 interacts with CLASP2 and NEK1 through coiled-coil regions and their cellular colocalization suggests centrosomal functions and regulation by PKC. Mol Cell Biochem. 2010;338:35-45 pubmed publisher
    FEZ1 was initially described as a neuronal protein that influences axonal development and cell polarization. CLASP2 and NEK1 proteins are present in a centrosomal complex and participate in cell cycle and cell division mechanisms, but ..
  34. Kruse R, Krantz J, Barker N, Coletta R, Rafikov R, Luo M, et al. Characterization of the CLASP2 Protein Interaction Network Identifies SOGA1 as a Microtubule-Associated Protein. Mol Cell Proteomics. 2017;16:1718-1735 pubmed publisher
    b>CLASP2 is a microtubule-associated protein that undergoes insulin-stimulated phosphorylation and co-localization with reorganized actin and GLUT4 at the plasma membrane...
  35. Zhu B, Qi L, Liu S, Liu W, Ou Z, Chen M, et al. CLASP2 is involved in the EMT and early progression after transurethral resection of the bladder tumor. BMC Cancer. 2017;17:105 pubmed publisher
    Cytoplasmic linker-associated protein 2 (CLASP2) belongs to a family of microtubule plus-end tracking proteins that localizes to the distal ends of microtubules and regulate microtubule dynamics...
  36. Pemble H, Kumar P, van Haren J, Wittmann T. GSK3-mediated CLASP2 phosphorylation modulates kinetochore dynamics. J Cell Sci. 2017;130:1404-1412 pubmed publisher
    ..One such protein is the cytoplasmic linker-associated protein 2 (CLASP2)...
  37. Xia P, Wang Z, Liu X, Wu B, Wang J, Ward T, et al. EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis. Proc Natl Acad Sci U S A. 2012;109:16564-9 pubmed publisher
    ..These results establish a previously uncharacterized regulatory mechanism governing localization of microtubule plus-end tracking proteins and thereby the plasticity and dynamics of cells. ..
  38. Matsui T, Watanabe T, Matsuzawa K, Kakeno M, Okumura N, Sugiyama I, et al. PAR3 and aPKC regulate Golgi organization through CLASP2 phosphorylation to generate cell polarity. Mol Biol Cell. 2015;26:751-61 pubmed publisher
    ..Here we demonstrate the functional cross-talk of the PAR complex with CLASP2, which is a microtubule plus-end-tracking protein and is involved in organizing the Golgi ribbon...
  39. Al Bassam J, Chang F. Regulation of microtubule dynamics by TOG-domain proteins XMAP215/Dis1 and CLASP. Trends Cell Biol. 2011;21:604-14 pubmed publisher
    ..These are structurally related proteins that use conserved TOG domains to recruit tubulin dimers to MTs. We discuss models for how these proteins might use these individual tubulin dimers to regulate dynamic behavior of MT plus ends. ..
  40. Engel U, Zhan Y, Long J, Boyle S, Ballif B, Dorey K, et al. Abelson phosphorylation of CLASP2 modulates its association with microtubules and actin. Cytoskeleton (Hoboken). 2014;71:195-209 pubmed publisher
    ..Using purified proteins, we find that Abl phosphorylation modulates direct binding between purified CLASP2 with both MTs and actin...
  41. Assmann E, Alborghetti M, Camargo M, Kobarg J. FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region. J Biol Chem. 2006;281:9869-81 pubmed
    ..Our results give further support to the previous speculation of the functional involvement of FEZ1 in neuronal development but suggest further that FEZ1 may also be involved in transcriptional control. ..
  42. Lim B, Matsumoto S, Yamamoto H, Mizuno H, Kikuta J, Ishii M, et al. Prickle1 promotes focal adhesion disassembly in cooperation with the CLASP-LL5? complex in migrating cells. J Cell Sci. 2016;129:3115-29 pubmed publisher
    ..CLASPs (of which there are two isoforms, CLASP1 and CLASP2, in mammals) and LL5? (also known as PHLDB2) have been reported to form a complex at cell edges and to control ..
  43. Limbeck E, Vanselow J, Hofmann J, Schlosser A, Mally A. Linking site-specific loss of histone acetylation to repression of gene expression by the mycotoxin ochratoxin A. Arch Toxicol. 2018;92:995-1014 pubmed publisher
    ..a possible link between H3K9 or H3K14 hypoacetylation and OTA-mediated down-regulation of selected genes (AMIGO2, CLASP2, CTNND1) confirmed OTA-mediated H3K9 hypoacetylation at promoter regions of these genes...