CIN85

Summary

Gene Symbol: CIN85
Description: SH3 domain containing kinase binding protein 1
Alias: CD2BP3, CIN85, GIG10, HSB-1, HSB1, MIG18, SH3 domain-containing kinase-binding protein 1, CD2-binding protein 3, SH3-domain kinase binding protein 1, Src family kinase-binding protein 1, c-Cbl-interacting protein, cbl-interacting protein of 85 kDa, human Src family kinase-binding protein 1, migration-inducing gene 18, src-related kinase binding protein-1
Species: human
Products:     CIN85

Top Publications

  1. Hutchings N, Clarkson N, Chalkley R, Barclay A, Brown M. Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85. J Biol Chem. 2003;278:22396-403 pubmed
    ..A peptide from this region (CD2:322-339) isolated CMS (human CD2AP); a related protein, CIN85; and the actin capping protein, CAPZ from a T cell line...
  2. Feng L, Wang J, Jin H, Qian K, Geng J. SH3KBP1-binding protein 1 prevents epidermal growth factor receptor degradation by the interruption of c-Cbl-CIN85 complex. Cell Biochem Funct. 2011;29:589-96 pubmed publisher
    The binding of Cbl-interacting protein of 85?kDa (CIN85) to c-Cbl is important to endocytosis and degradation of epidermal growth factor receptor (EGFR)...
  3. Kowanetz K, Husnjak K, Höller D, Kowanetz M, Soubeyran P, Hirsch D, et al. CIN85 associates with multiple effectors controlling intracellular trafficking of epidermal growth factor receptors. Mol Biol Cell. 2004;15:3155-66 pubmed
    b>CIN85 is a multidomain adaptor protein involved in Cbl-mediated down-regulation of epidermal growth factor (EGF) receptors...
  4. Kowanetz K, Terzic J, Dikic I. Dab2 links CIN85 with clathrin-mediated receptor internalization. FEBS Lett. 2003;554:81-7 pubmed
    b>CIN85 is a multidomain scaffold protein involved in downregulation of receptor tyrosine kinases...
  5. Haglund K, Shimokawa N, Szymkiewicz I, Dikic I. Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors. Proc Natl Acad Sci U S A. 2002;99:12191-6 pubmed
    ..They also recruit CIN85-endophilin in the complex with activated receptors, thus controlling receptor endocytosis...
  6. Moncalian G, Cárdenes N, Deribe Y, Spínola Amilibia M, Dikic I, Bravo J. Atypical polyproline recognition by the CMS N-terminal Src homology 3 domain. J Biol Chem. 2006;281:38845-53 pubmed
    The CIN85/CMS (human homologs of mouse SH3KBP1/CD2AP) family of endocytic adaptor proteins has the ability to engage multiple effectors and couple cargo trafficking with the cytoskeleton...
  7. Usami Y, Popov S, Gottlinger H. Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site. J Virol. 2007;81:6614-22 pubmed
    ..also depends on its C-terminal proline-rich domain (PRD), but not on the binding sites for Tsg101, endophilin, CIN85, or for the newly identified binding partner, CMS, within the PRD...
  8. Korzhnev D, Bezsonova I, Lee S, Chalikian T, Kay L. Alternate binding modes for a ubiquitin-SH3 domain interaction studied by NMR spectroscopy. J Mol Biol. 2009;386:391-405 pubmed publisher
    ..In this study, we used NMR spectroscopy to study the interaction between the C-terminal SH3 domain of CIN85 and ubiquitin that involves the 'classical' binding sites of these proteins...
  9. Chandra V, Kalia M, Hajela K, Jameel S. The ORF3 protein of hepatitis E virus delays degradation of activated growth factor receptors by interacting with CIN85 and blocking formation of the Cbl-CIN85 complex. J Virol. 2010;84:3857-67 pubmed publisher
    ..The ORF3 protein was found to interact with CIN85, a multidomain adaptor protein implicated in the Cbl-mediated downregulation of receptor tyrosine kinases...

More Information

Publications83

  1. Schmidt M, Chen B, Randazzo L, Bogler O. SETA/CIN85/Ruk and its binding partner AIP1 associate with diverse cytoskeletal elements, including FAKs, and modulate cell adhesion. J Cell Sci. 2003;116:2845-55 pubmed
    The adaptor protein SETA/CIN85/Ruk is involved in regulating diverse signal transduction pathways, including the internalization of tyrosine kinase receptors via the Cbl ubiquitin ligases, and attenuating PI3K activity by interaction ..
  2. Schroeder B, Weller S, Chen J, BILLADEAU D, McNiven M. A Dyn2-CIN85 complex mediates degradative traffic of the EGFR by regulation of late endosomal budding. EMBO J. 2010;29:3039-53 pubmed publisher
    ..The adaptor protein CIN85 (cbl-interacting protein of 85 kDa), which has been proposed to indirectly link the EGFR to the endocytic ..
  3. Kowanetz K, Szymkiewicz I, Haglund K, Kowanetz M, Husnjak K, Taylor J, et al. Identification of a novel proline-arginine motif involved in CIN85-dependent clustering of Cbl and down-regulation of epidermal growth factor receptors. J Biol Chem. 2003;278:39735-46 pubmed
    b>CIN85 is a multidomain adaptor protein implicated in Cbl-mediated down-regulation of receptor tyrosine kinases...
  4. Jozic D, Cárdenes N, Deribe Y, Moncalian G, Hoeller D, Groemping Y, et al. Cbl promotes clustering of endocytic adaptor proteins. Nat Struct Mol Biol. 2005;12:972-9 pubmed
    ..Their function is modulated through interactions with regulatory proteins including CIN85 and PIX, which recognize a proline-arginine motif in Cbl and thus promote or inhibit receptor endocytosis...
  5. Schmidt M, Hoeller D, Yu J, Furnari F, Cavenee W, Dikic I, et al. Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex. Mol Cell Biol. 2004;24:8981-93 pubmed
    The assembly of the Cbl-SETA/CIN85-endophilin complex at the C terminus of the epidermal growth factor receptor (EGFR) following ligand activation mediates its internalization and ubiquitination...
  6. Ceregido M, Garcia Pino A, Ortega Roldan J, Casares S, Lopez Mayorga O, Bravo J, et al. Multimeric and differential binding of CIN85/CD2AP with two atypical proline-rich sequences from CD2 and Cbl-b*. FEBS J. 2013;280:3399-415 pubmed publisher
    The CD2AP (CD2-associated protein) and CIN85 (Cbl-interacting protein of 85 kDa) adaptor proteins each employ three Src homology 3 (SH3) domains to cluster protein partners and ensure efficient signal transduction and down-regulation of ..
  7. Gout I, Middleton G, Adu J, Ninkina N, Drobot L, Filonenko V, et al. Negative regulation of PI 3-kinase by Ruk, a novel adaptor protein. EMBO J. 2000;19:4015-25 pubmed
    ..Structural similarities between Ruk, CIN85 and CD2AP/CMS suggest that these proteins form a novel family of adaptor molecules that are involved in various ..
  8. Petrelli A, Gilestro G, Lanzardo S, Comoglio P, Migone N, Giordano S. The endophilin-CIN85-Cbl complex mediates ligand-dependent downregulation of c-Met. Nature. 2002;416:187-90 pubmed
    ..Here we demonstrate that a complex involving endophilins, CIN85 and Cbl controls this process. Endophilins are regulatory components of clathrin-coated vesicle formation...
  9. Narita T, Nishimura T, Yoshizaki K, Taniyama T. CIN85 associates with TNF receptor 1 via Src and modulates TNF-alpha-induced apoptosis. Exp Cell Res. 2005;304:256-64 pubmed
    b>CIN85 is a multidomain protein that associates with receptors carrying tyrosine kinase domains...
  10. Stamenova S, French M, He Y, Francis S, Kramer Z, Hicke L. Ubiquitin binds to and regulates a subset of SH3 domains. Mol Cell. 2007;25:273-84 pubmed
    ..The yeast endocytic protein Sla1, as well as the mammalian proteins CIN85 and amphiphysin, carry ubiquitin-binding SH3 domains...
  11. Nam J, Onodera Y, Mazaki Y, Miyoshi H, Hashimoto S, Sabe H. CIN85, a Cbl-interacting protein, is a component of AMAP1-mediated breast cancer invasion machinery. EMBO J. 2007;26:647-56 pubmed
    ..A mouse ortholog of AMAP1, ASAP1, is known to bind to CIN85, a binding partner of an E3 ligase, Cbl...
  12. Watanabe S, Take H, Takeda K, Yu Z, Iwata N, Kajigaya S. Characterization of the CIN85 adaptor protein and identification of components involved in CIN85 complexes. Biochem Biophys Res Commun. 2000;278:167-74 pubmed
    b>CIN85 is an 85-kDa adaptor protein whose functions in signaling pathways are presently unknown. Using the yeast two-hybrid screen, the B cell linker protein (BLNK) was identified as a binding partner of CIN85...
  13. Gaidos G, Soni S, Oswald D, Toselli P, Kirsch K. Structure and function analysis of the CMS/CIN85 protein family identifies actin-bundling properties and heterotypic-complex formation. J Cell Sci. 2007;120:2366-77 pubmed
    Members of the CMS/CIN85 protein family participate in clathrin-mediated endocytosis and play a crucial role in maintaining the kidney filtration barrier...
  14. Zhang J, Zheng X, Yang X, Liao K. CIN85 associates with endosomal membrane and binds phosphatidic acid. Cell Res. 2009;19:733-46 pubmed publisher
    b>CIN85 (Cbl-interacting protein of 85 kDa) is an important molecule involved in receptor tyrosine kinase endocytosis...
  15. Tibaldi E, Reinherz E. CD2BP3, CIN85 and the structurally related adaptor protein CMS bind to the same CD2 cytoplasmic segment, but elicit divergent functional activities. Int Immunol. 2003;15:313-29 pubmed
    Interaction trap cloning was used to identify a CD2 cytoplasmic tail-binding protein termed CD2BP3. CD2BP3 is the major RNA splice variant of the CIN85 locus in human T lymphocytes, lacking SH3A, the first of three SH3 domains found in ..
  16. Soubeyran P, Kowanetz K, Szymkiewicz I, Langdon W, Dikic I. Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors. Nature. 2002;416:183-7 pubmed
    ..Cbl rapidly recruits CIN85 (Cbl-interacting protein of 85K; ref...
  17. Aissouni Y, Zapart G, Iovanna J, Dikic I, Soubeyran P. CIN85 regulates the ability of MEKK4 to activate the p38 MAP kinase pathway. Biochem Biophys Res Commun. 2005;338:808-14 pubmed
    b>CIN85 is a multi-adaptor protein involved in different cellular functions including the down-regulation of activated receptor tyrosine kinases and survival of neuronal cells...
  18. Rønning S, Pedersen N, Madshus I, Stang E. CIN85 regulates ubiquitination and degradative endosomal sorting of the EGF receptor. Exp Cell Res. 2011;317:1804-16 pubmed publisher
    b>CIN85 has been demonstrated to interact with a number of proteins involved in endocytosis and intracellular sorting. However, the exact functional role of CIN85 in endocytosis remains unclear...
  19. Bezsonova I, Bruce M, Wiesner S, Lin H, Rotin D, Forman Kay J. Interactions between the three CIN85 SH3 domains and ubiquitin: implications for CIN85 ubiquitination. Biochemistry. 2008;47:8937-49 pubmed publisher
    b>CIN85 is an adaptor protein linking the ubiquitin ligase Cbl and clathrin-binding proteins in clathrin-mediated receptor endocytosis. The SH3 domains of CIN85 bind to a proline-rich region of Cbl...
  20. Verdier F, Valovka T, Zhyvoloup A, Drobot L, Buchman V, Waterfield M, et al. Ruk is ubiquitinated but not degraded by the proteasome. Eur J Biochem. 2002;269:3402-8 pubmed
    The regulator of ubiquitous kinase (Ruk) protein, also known as CIN85 or SETA, is an adaptor-type protein belonging to the CD2AP/CMS family...
  21. Szymkiewicz I, Kowanetz K, Soubeyran P, Dinarina A, Lipkowitz S, Dikic I. CIN85 participates in Cbl-b-mediated down-regulation of receptor tyrosine kinases. J Biol Chem. 2002;277:39666-72 pubmed
    ..More recently, a novel pathway has been identified whereby Cbl promotes internalization of EGF receptor via a CIN85/endophilin pathway that is functionally separable from the ubiquitin ligase activity of Cbl (1)...
  22. Kurakin A, Wu S, Bredesen D. Atypical recognition consensus of CIN85/SETA/Ruk SH3 domains revealed by target-assisted iterative screening. J Biol Chem. 2003;278:34102-9 pubmed
    ..screening applied to random peptide libraries unveiled a novel and atypical recognition consensus shared by CIN85/SETA/Ruk SH3 domains, PX(P/A)XXR...
  23. Take H, Watanabe S, Takeda K, Yu Z, Iwata N, Kajigaya S. Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl. Biochem Biophys Res Commun. 2000;268:321-8 pubmed
    ..We have identified a novel c-Cbl-interacting protein termed CIN85 with a molecular mass of 85 kDa which shows similarity to adaptor proteins, CMS and CD2AP...
  24. Chen B, Borinstein S, Gillis J, Sykes V, Bogler O. The glioma-associated protein SETA interacts with AIP1/Alix and ALG-2 and modulates apoptosis in astrocytes. J Biol Chem. 2000;275:19275-81 pubmed
    ..Expression of the isolated SH3-N domain had the greatest effect in these experiments, indicating that interference in the interaction between endogenous SETA and AIP1 sensitizes astrocytes to apoptosis in response to DNA damage. ..
  25. Borinstein S, Hyatt M, Sykes V, Straub R, Lipkowitz S, Boulter J, et al. SETA is a multifunctional adapter protein with three SH3 domains that binds Grb2, Cbl, and the novel SB1 proteins. Cell Signal. 2000;12:769-79 pubmed
    ..Evidence that SETA binds to the CD2 protein, the proto-oncogene c-Cbl, and the signal transduction molecule Grb2, and can dimerize via its C-terminal coiled coil (CC) domain is also presented. ..
  26. Oellerich T, Bremes V, Neumann K, Bohnenberger H, Dittmann K, Hsiao H, et al. The B-cell antigen receptor signals through a preformed transducer module of SLP65 and CIN85. EMBO J. 2011;30:3620-34 pubmed publisher
    ..However, a stimulation-independent and constant association of SLP65 with the Cbl-interacting protein of 85 kDa (CIN85) was requisite for SLP65 phosphorylation and its inducible plasma membrane translocation...
  27. Stintzing S, Zhang W, Heinemann V, Neureiter D, Kemmerling R, Kirchner T, et al. Polymorphisms in Genes Involved in EGFR Turnover Are Predictive for Cetuximab Efficacy in Colorectal Cancer. Mol Cancer Ther. 2015;14:2374-81 pubmed publisher
    ..This is the first report suggesting that germline polymorphisms in the degradation process predict efficacy of cetuximab in patients with mCRC. Genes involved in EGFR turnover may be new targets in the treatment of mCRC. ..
  28. Tossidou I, Teng B, Drobot L, Meyer Schwesinger C, Worthmann K, Haller H, et al. CIN85/RukL is a novel binding partner of nephrin and podocin and mediates slit diaphragm turnover in podocytes. J Biol Chem. 2010;285:25285-95 pubmed publisher
    ..In this manuscript we examined a mechanism of nephrin endocytosis via CIN85/Ruk(L)-mediated ubiquitination...
  29. Niiro H, Jabbarzadeh Tabrizi S, Kikushige Y, Shima T, Noda K, Ota S, et al. CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in human B cells. Blood. 2012;119:2263-73 pubmed publisher
    ..Here, we demonstrate that CIN85 (c-Cbl interacting protein of 85 kDa) is constitutively associated with c-Cbl, Cbl-b, and B-cell linker in B cells...
  30. Bior B, Ballif B. Dab1 stabilizes its interaction with Cin85 by suppressing Cin85 phosphorylation at serine 587. FEBS Lett. 2013;587:60-6 pubmed publisher
    ..Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1...
  31. Schmidt M, Husnjak K, Szymkiewicz I, Haglund K, Dikic I. Cbl escapes Cdc42-mediated inhibition by downregulation of the adaptor molecule betaPix. Oncogene. 2006;25:3071-8 pubmed
    ..Such a relationship provides a mechanism to fine-tune the kinetics of RTK endocytosis and degradation depending on the pool of active Cdc42 and the duration of EGFR signaling. ..
  32. Ababou A, Pfuhl M, Ladbury J. Novel insights into the mechanisms of CIN85 SH3 domains binding to Cbl proteins: solution-based investigations and in vivo implications. J Mol Biol. 2009;387:1120-36 pubmed publisher
    b>CIN85 is a multifunctional protein that plays key roles in endocytic down-regulation of receptor tyrosine kinases, apoptosis, cell adhesion, and cytoskeleton rearrangement...
  33. Nikolaienko O, Skrypkina I, Tsyba L, Fedyshyn Y, Morderer D, Buchman V, et al. Intersectin 1 forms a complex with adaptor protein Ruk/CIN85 in vivo independently of epidermal growth factor stimulation. Cell Signal. 2009;21:753-9 pubmed publisher
    ..Here, we show that ITSN1 forms a complex with adaptor protein Ruk/CIN85, implicated in downregulation of receptor tyrosine kinases...
  34. Molfetta R, Belleudi F, Peruzzi G, Morrone S, Leone L, Dikic I, et al. CIN85 regulates the ligand-dependent endocytosis of the IgE receptor: a new molecular mechanism to dampen mast cell function. J Immunol. 2005;175:4208-16 pubmed
    ..molecular mechanisms involved in attenuating Fc epsilonRI expression and signaling, we focused our attention on CIN85, a scaffold molecule that regulates, in concert with the ubiquitin ligase Cbl, the clathrin-mediated endocytosis ..
  35. Ortega Roldan J, Casares S, Ringkjøbing Jensen M, Cárdenes N, Bravo J, Blackledge M, et al. Distinct ubiquitin binding modes exhibited by SH3 domains: molecular determinants and functional implications. PLoS ONE. 2013;8:e73018 pubmed publisher
    ..We found that the Phe-to-Tyr mutation in CD2AP and in the homologous CIN85 SH3-C domain does not abrogate ubiquitin binding, in contrast to previous hypothesis and our findings for the ..
  36. Samoylenko A, Dimova E, Kozlova N, Drobot L, Kietzmann T. The adaptor protein Ruk/CIN85 activates plasminogen activator inhibitor-1 (PAI-1) expression via hypoxia-inducible factor-1alpha. Thromb Haemost. 2010;103:901-9 pubmed publisher
    ..The adaptor/scaffold protein Ruk/CIN85 may be a candidate since it plays important roles in the regulation of processes associated with cardiovascular ..
  37. Vynnytska Myronovska B, Bobak Y, Pasichnyk G, Igumentseva N, Samoylenko A, Drobot L. Multiple molecular forms of adaptor protein Ruk/CIN85 specifically associate with different subcellular compartments in human breast adenocarcinoma MCF-7 cells. Ukr Biochem J. 2014;86:102-10 pubmed
    Ruk/CIN85 is a receptor-proximal 'signalling' adaptor that possesses three SH3 domains, Pro- and Ser-rich regions and C-terminal coiled-coil domain...
  38. Konishi H, Tashiro K, Murata Y, Nabeshi H, Yamauchi E, Taniguchi H. CFBP is a novel tyrosine-phosphorylated protein that might function as a regulator of CIN85/CD2AP. J Biol Chem. 2006;281:28919-31 pubmed
    ..Here, we focus on characterizing a novel protein, CFBP (CIN85/CD2AP family binding protein), identified in the study...
  39. Wells C, Fawcett J, Traweger A, Yamanaka Y, Goudreault M, Elder K, et al. A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells. Cell. 2006;125:535-48 pubmed
    ..We propose that Rich1 and Amot maintain TJ integrity by the coordinate regulation of Cdc42 and by linking specific components of the TJ to intracellular protein trafficking. ..
  40. Havrylov S, Ichioka F, Powell K, Borthwick E, Baranska J, Maki M, et al. Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of the Golgi complex. Traffic. 2008;9:798-812 pubmed publisher
    Regulator of ubiquitous kinase/Cbl-interacting protein of 85 kDa (Ruk/CIN85) and CD2-associated protein/Cas ligand with multiple SH3 domains (CD2AP/CMS) comprise a family of vertebrate adaptor proteins involved in several important ..
  41. Lito P, Mets B, Kleff S, O Reilly S, Maher V, McCormick J. Evidence that sprouty 2 is necessary for sarcoma formation by H-Ras oncogene-transformed human fibroblasts. J Biol Chem. 2008;283:2002-9 pubmed
    ..cells revealed that Spry2 and H-Ras interact and that H-Ras interacts with Spry2-binding partners, c-Cbl and CIN85, in a Spry2-dependent manner...
  42. Borthwick E, Korobko I, Luke C, Drel V, Fedyshyn Y, Ninkina N, et al. Multiple domains of Ruk/CIN85/SETA/CD2BP3 are involved in interaction with p85alpha regulatory subunit of PI 3-kinase. J Mol Biol. 2004;343:1135-46 pubmed
    Ruk/CIN85/SETA/CD2BP3 and CD2AP/CMS/METS-1 comprise a new family of proteins involved in such fundamental processes as clustering of receptors and rearrangement of the cytoskeleton in regions of specialised cell-cell contacts, ligand-..
  43. Yang S, Qu S, Perez Tores M, Sawai A, Rosen N, Solit D, et al. Association with HSP90 inhibits Cbl-mediated down-regulation of mutant epidermal growth factor receptors. Cancer Res. 2006;66:6990-7 pubmed
    ..were constitutively associated with the E3 ubiquitin ligase Cbl but did not associate with the adaptor protein CIN85 on the addition of ligand...
  44. Le Bras S, Moon C, Foucault I, Breittmayer J, Deckert M. Abl-SH3 binding protein 2, 3BP2, interacts with CIN85 and HIP-55. FEBS Lett. 2007;581:967-74 pubmed
    ..Here, we show that 3BP2 directly interacts with the endocytic scaffold protein CIN85 and the actin-binding protein HIP-55...
  45. Yasin H, van Rensburg S, Feiler C, Johnson R. The adaptor protein Cindr regulates JNK activity to maintain epithelial sheet integrity. Dev Biol. 2016;410:135-149 pubmed publisher
    ..Here we show that Cindr (the Drosophila Cin85 and Cd2ap ortholog) is required to maintain epithelial integrity...
  46. Johnson R, Seppa M, Cagan R. The Drosophila CD2AP/CIN85 orthologue Cindr regulates junctions and cytoskeleton dynamics during tissue patterning. J Cell Biol. 2008;180:1191-204 pubmed publisher
    ..Here, we identify one candidate, Cindr, the sole Drosophila melanogaster member of the CD2AP/CIN85 family (this family has been previously implicated in a variety of processes). Using D...
  47. Ichioka F, Takaya E, Suzuki H, Kajigaya S, Buchman V, Shibata H, et al. HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions. Arch Biochem Biophys. 2007;457:142-9 pubmed
    ..endocytosis, endosomal protein sorting and cell adhesion by associating with various proteins such as ALG-2, CIN85/Rukl/SETA, endophilins, CHMP4s and TSG101...
  48. Yakymovych I, Yakymovych M, Zang G, Mu Y, Bergh A, Landström M, et al. CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface. J Cell Biol. 2015;210:319-32 pubmed publisher
    ..We report here that TβRI interacts with the SH3 domains of the adaptor protein CIN85 in response to TGFβ stimulation in a TRAF6-dependent manner...
  49. Kit Y, Drel V, Petriv O, Kovalyova V, Shuvaeva G, Palivoda O, et al. Adaptor protein Ruk1 forms protein-protein complexes with endonuclease activity in HEK293 cells. Biochemistry (Mosc). 2003;68:810-5 pubmed
    ..It was concluded that the adaptor protein Ruk(1) forms complexes with endonucleases in HEK293 cells. ..
  50. Minegishi Y, Shibagaki Y, Mizutani A, Fujita K, Tezuka T, Kinoshita M, et al. Adaptor protein complex of FRS2? and CIN85/CD2AP provides a novel mechanism for ErbB2/HER2 protein downregulation. Cancer Sci. 2013;104:345-52 pubmed publisher
    ..ErbB2 constitutively binds to an adaptor protein FRS2?. We found that FRS2? bound to CD2AP and CIN85, which induces endosomal trafficking that targets lysosomes. FRS2? colocalized with CIN85 in the cytoplasm...
  51. Kim J, Kang D, Sun B, Kim J, Song J. TRAIL/MEKK4/p38/HSP27/Akt survival network is biphasically modulated by the Src/CIN85/c-Cbl complex. Cell Signal. 2013;25:372-9 pubmed publisher
    ..shock protein (HSP27)/Akt survival network is modulated by the Src/c-Cbl interacting protein of 85kDa (CIN85)/c-Cbl complex...
  52. Li J, Nishizawa K, An W, Hussey R, Lialios F, Salgia R, et al. A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion. EMBO J. 1998;17:7320-36 pubmed
    ..These findings offer the first molecular view into the control processes for T cell adhesion. ..
  53. Bazalii A, Horak I, Pasi chn yk G, Komisarenko S, Drobot L. Transcriptional regulation of NOX genes express ion in human breast adenocarcinoma MCF-7 cells is modulated by adaptor protein Ruk/CIN 85. Ukr Biochem J. 2016;88:119-25 pubmed publisher
  54. Philippe D, Ababou A, Yang X, Ghosh R, Daviter T, Ladbury J, et al. Making ends meet: the importance of the N- and C-termini for the structure, stability, and function of the third SH3 domain of CIN85. Biochemistry. 2011;50:3649-59 pubmed publisher
    ..In this report, we studied the third SH3 domain from the human CIN85 adaptor protein, which plays an important role in both receptor tyrosine kinase downregulation and ..
  55. Kedar V, Darby M, Williams J, Blackshear P. Phosphorylation of human tristetraprolin in response to its interaction with the Cbl interacting protein CIN85. PLoS ONE. 2010;5:e9588 pubmed publisher
    ..Among these, the interactions between hTTP and CIN85, cytoplasmic poly (A) binding protein (PABP), nucleolin and heat shock protein 70 were confirmed by co-..
  56. Liang Y, Kurakin A, Roizman B. Herpes simplex virus 1 infected cell protein 0 forms a complex with CIN85 and Cbl and mediates the degradation of EGF receptor from cell surfaces. Proc Natl Acad Sci U S A. 2005;102:5838-43 pubmed
    ..between residues 245 and 510 contains multiple SH3 domain binding motifs similar to those required for binding to CIN85, the M(r) 85,000 protein that interacts with Cbl...
  57. Kawata A, Iida J, Ikeda M, Sato Y, Mori H, Kansaku A, et al. CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. J Biochem. 2006;139:931-9 pubmed
    ..We identified CIN85, an endocytic scaffold protein, as a putative dendrin-interactor...
  58. Sairenji T, Ikezawa J, Kaneko R, Masuda S, Uchida K, Takanashi Y, et al. Maternal prolactin during late pregnancy is important in generating nurturing behavior in the offspring. Proc Natl Acad Sci U S A. 2017;114:13042-13047 pubmed publisher
    ..We found that Cin85-deficient (Cin85-/-) mother mice had reduced pituitary hormone prolactin (PRL) secretion as a ..
  59. Condé C, Rambout X, Lebrun M, Lecat A, Di Valentin E, Dequiedt F, et al. The inositol phosphatase SHIP-1 inhibits NOD2-induced NF-?B activation by disturbing the interaction of XIAP with RIP2. PLoS ONE. 2012;7:e41005 pubmed publisher
    ..Upon NOD2 activation, SHIP-1 C-terminal proline rich domain (PRD) interacts with XIAP, thereby disturbing the interaction between XIAP and RIP2 in order to decrease NF-?B signaling. ..
  60. Schmidt M, Furnari F, Cavenee W, Bogler O. Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization. Proc Natl Acad Sci U S A. 2003;100:6505-10 pubmed
    ..expressed in tumorigenic astrocytes/Cbl-interacting protein of 85 kDa/regulator of ubiquitous kinase (SETA/CIN85/Ruk)...
  61. Narita T, Ando A, Mikami Y, Taniyama T. Overexpression of CIN85 suppresses the growth of herpes simplex virus in HeLa cells. Exp Cell Res. 2005;311:265-71 pubmed
    The adaptor protein CIN85 is widely distributed in different tissues and has three Src homology 3 (SH3) domains, a proline-rich region (PRR), and a coiled-coil domain...
  62. Liang Y, Roizman B. State and role of SRC family kinases in replication of herpes simplex virus 1. J Virol. 2006;80:3349-59 pubmed publisher
    ..ICP0) of herpes simplex virus 1 (HSV-1) interacts with the SH3 domains of a recently discovered adaptor protein, CIN85. Here, we report the following...
  63. Deschamps T, Dogrammatzis C, Mullick R, Kalamvoki M. Cbl E3 Ligase Mediates the Removal of Nectin-1 from the Surface of Herpes Simplex Virus 1-Infected Cells. J Virol. 2017;91: pubmed publisher
    ..The adaptor protein CIN85 is a partner of Cbl that augments many of these interactions...
  64. Yoon H, Kales S, Luo R, Lipkowitz S, Randazzo P. ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking. Biol Cell. 2011;103:171-84 pubmed publisher
    ..Here we report that ARAP1 associates with the CIN85 (Cbl-interacting protein of 85 kDa)...
  65. Tossidou I, Niedenthal R, Klaus M, Teng B, Worthmann K, King B, et al. CD2AP regulates SUMOylation of CIN85 in podocytes. Mol Cell Biol. 2012;32:1068-79 pubmed publisher
    ..Together with its paralogue, CIN85, CD2AP belongs to a family of adaptor proteins that are primarily described as being involved in endocytosis and ..
  66. Diesenberg K, Beerbaum M, Fink U, Schmieder P, Krauss M. SEPT9 negatively regulates ubiquitin-dependent downregulation of EGFR. J Cell Sci. 2015;128:397-407 pubmed publisher
    ..a consensus motif within the SEPT9 N-terminal domain that supports its association with the adaptor protein CIN85 (also known as SH3KBP1)...
  67. Selbach M, Mann M. Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK). Nat Methods. 2006;3:981-3 pubmed
    ..We used this screen to identify interaction partners of beta-catenin and Cbl. ..
  68. Bushnell H, Feiler C, Ketosugbo K, Hellerman M, Nazzaro V, Johnson R. JNK is antagonized to ensure the correct number of interommatidial cells pattern the Drosophila retina. Dev Biol. 2018;433:94-107 pubmed publisher
    ..However, we found that JNK activity is repressed by Cindr (the CIN85 and CD2AP ortholog) in order to promote cell survival. Reducing the amount of Cindr resulted in ectopic cell death...
  69. Tsunematsu T, Yamauchi E, Shibata H, Maki M, Ohta T, Konishi H. Distinct functions of human MVB12A and MVB12B in the ESCRT-I dependent on their posttranslational modifications. Biochem Biophys Res Commun. 2010;399:232-7 pubmed publisher
    ..These results suggest that the expression of MVB12B may be normally suppressed through the ubiquitin-proteasome pathway that simultaneously regulates the fate of MVB12A and the functions of ESCRT-I. ..
  70. Shimokawa N, Haglund K, Holter S, Grabbe C, Kirkin V, Koibuchi N, et al. CIN85 regulates dopamine receptor endocytosis and governs behaviour in mice. EMBO J. 2010;29:2421-32 pubmed publisher
    Despite extensive investigations of Cbl-interacting protein of 85 kDa (CIN85) in receptor trafficking and cytoskeletal dynamics, little is known about its functions in vivo...
  71. Rauch S, Martin Serrano J. Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding. J Virol. 2011;85:3546-56 pubmed publisher
    ..Taken together, our results support a model whereby ARTs are involved in PPXY-mediated budding by interacting with HECT ubiquitin ligases and providing several alternative routes for ESCRT-III recruitment. ..
  72. Wakasaki T, Masuda M, Niiro H, Jabbarzadeh Tabrizi S, Noda K, Taniyama T, et al. A critical role of c-Cbl-interacting protein of 85 kDa in the development and progression of head and neck squamous cell carcinomas through the ras-ERK pathway. Neoplasia. 2010;12:789-96 pubmed
    ..c-Cbl-interacting protein of 85 kDa (CIN85) is an adaptor protein that facilitates EGFR internalization...
  73. Carlton J, Agromayor M, Martin Serrano J. Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release. Proc Natl Acad Sci U S A. 2008;105:10541-6 pubmed publisher
    ..Last, we demonstrate that overexpression of Chmp4b and Chmp4c differentially inhibits HIV-1 release and cytokinesis, suggesting possible reasons for gene expansion within the mammalian Class E VPS pathway. ..
  74. Kühn J, Wong L, Pirkuliyeva S, Schulz K, Schwiegk C, Fünfgeld K, et al. The adaptor protein CIN85 assembles intracellular signaling clusters for B cell activation. Sci Signal. 2016;9:ra66 pubmed publisher
    The adaptor molecule Cbl-interacting protein of 85 kD (CIN85) regulates signaling from a number of cell surface receptors, such as growth factor receptors and antigen receptors on lymphocytes...