Gene Symbol: CHST5
Description: carbohydrate sulfotransferase 5
Alias: I-GlcNAc-6-ST, I-GlcNAc6ST, glcNAc6ST-3, gn6st-3, hIGn6ST, carbohydrate sulfotransferase 5, GST4-alpha, N-acetylglucosamine 6-O-sulfotransferase 3, carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha, intestinal GlcNAc-6-sulfotransferase
Species: human
Products:     CHST5

Top Publications

  1. Narentuya -, Takeda Uchimura Y, Foyez T, ZHANG Z, Akama T, Yagi H, et al. GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain. Sci Rep. 2019;9:4387 pubmed publisher
    ..Surprisingly, GlcNAc6ST3, which was discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood...
  2. Low S, Hirakawa J, Hoshino H, Uchimura K, Kawashima H, Kobayashi M. Role of MAdCAM-1-Expressing High Endothelial Venule-Like Vessels in Colitis Induced in Mice Lacking Sulfotransferases Catalyzing L-Selectin Ligand Biosynthesis. J Histochem Cytochem. 2018;66:415-425 pubmed publisher
  3. Yu S, Hsiao C, Izawa M, Yusa A, Ishida H, Nakamura S, et al. Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry-based sulfoglycomic analysis. J Biol Chem. 2018;293:15163-15177 pubmed publisher
    ..On the contrary, GlcNAc6ST-3 (CHST5) was only expressed in nonmalignant epithelial cells, whereas GlcNAc6ST-1 (CHST2) was expressed equally ..
  4. Parfitt G, Pinali C, Akama T, Young R, Nishida K, Quantock A, et al. Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas. J Struct Biol. 2011;174:536-41 pubmed publisher
    ..microscopy reconstructions of collagen-proteoglycan interactions in the anterior, mid and posterior stroma from a Chst5 knockout mouse, which lacks a keratan sulphate sulphotransferase, were obtained...
  5. Liu J, Jin C, Cherian R, Karlsson N, Holgersson J. O-glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O-glycan core enzyme cDNAs. J Biotechnol. 2015;199:77-89 pubmed publisher
    ..They will become important tools for assessing the core chain-dependent binding activity of carbohydrate-binding proteins. ..
  6. Taga M, Hoshino H, Low S, Imamura Y, Ito H, Yokoyama O, et al. A potential role for 6-sulfo sialyl Lewis X in metastasis of bladder urothelial carcinoma. Urol Oncol. 2015;33:496.e1-9 pubmed publisher
  7. Hoshino H, Ohta M, Ito M, Uchimura K, Sakai Y, Uehara T, et al. Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma. Lab Invest. 2016;96:1246-1255 pubmed publisher
  8. Akama T, Misra A, Hindsgaul O, Fukuda M. Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate. J Biol Chem. 2002;277:42505-13 pubmed
    ..hCGn6ST, also known as human GlcNAc6ST-5/GST4beta) and human intestinal GlcNAc 6-O-sulfotransferase (hIGn6ST or human GlcNAc6ST-3/GST4alpha) are highly homologous...
  9. Akama T, Nishida K, Nakayama J, Watanabe H, Ozaki K, Nakamura T, et al. Macular corneal dystrophy type I and type II are caused by distinct mutations in a new sulphotransferase gene. Nat Genet. 2000;26:237-41 pubmed
    ..In situ hybridization analysis did not detect CHST6 transcripts in corneal epithelium in an MCD type II patient, suggesting that the mutations found in type II lead to loss of cornea-specific expression of CHST6. ..

More Information


  1. Bernstein H, Compans R. Sulfation of the human immunodeficiency virus envelope glycoprotein. J Virol. 1992;66:6953-9 pubmed
    ..Sulfation is a major determinant of negative charge and could play a role in biological functions and antigenic properties of HIV glycoproteins. ..
  2. Kitayama K, Hayashida Y, Nishida K, Akama T. Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans. J Biol Chem. 2007;282:30085-96 pubmed
  3. Bartes A, Bhakta S, Hemmerich S. Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta). Biochem Biophys Res Commun. 2001;282:928-33 pubmed
    ..While I-GlcNAc6T is expressed mostly in intestinal tissue, larger C-GlcNAc6ST transcripts are found predominantly in the brain. ..
  4. Seko A, Nagata K, Yonezawa S, Yamashita K. Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma. Glycobiology. 2002;12:379-88 pubmed
    ..From these results, it was indicated that GlcNAc6ST-2 corresponds to mucinous adenocarcinoma-specific SulT-b and that expression of GlcNAc6ST-3 is down-regulated in colonic adenocarcinomas. ..
  5. Lee J, Bistrup A, van Zante A, Rosen S. Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications. Glycobiology. 2003;13:245-54 pubmed
    ..These latter findings argue that GlcNAc6ST-3 is not involved in generating HEV-expressed ligands for L-selectin. ..
  6. de Graffenried C, Bertozzi C. Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis. J Biol Chem. 2003;278:40282-95 pubmed
    ..We propose a model in which Golgi enzyme localization and competition orchestrate the biosynthesis of L-selectin ligands. ..
  7. Lee J, Bhakta S, Rosen S, Hemmerich S. Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue. Biochem Biophys Res Commun. 1999;263:543-9 pubmed
    ..Based on these characteristics, the novel sulfotransferase is designated I-GlcNAc6ST for intestinal GlcNAc 6-O-sulfotransferase. ..
  8. Shilatifard A, Merkle R, Helland D, Welles J, Haseltine W, Cummings R. Complex-type N-linked oligosaccharides of gp120 from human immunodeficiency virus type 1 contain sulfated N-acetylglucosamine. J Virol. 1993;67:943-52 pubmed
    ..Charge analysis of the [3H]galactose- and [3H]glucosamine-labeled glycopeptides from gp120 and gp160 indicates that approximately 14% of the complex-type N-linked oligosaccharides are sulfated. ..