Gene Symbol: CHMP6
Description: charged multivesicular body protein 6
Alias: VPS20, charged multivesicular body protein 6, chromatin-modifying protein 6, hVps20, vacuolar protein sorting-associated protein 20
Species: human
Products:     CHMP6

Top Publications

  1. Fu D, Tian L, Peng Z, Deng W, Yuan J, Ma D, et al. Overexpression of CHMP6 induces cellular oncosis and apoptosis in HeLa cells. Biosci Biotechnol Biochem. 2009;73:494-501 pubmed
    ..In this study, we found that overexpression of chromatin modifying protein 6 (CHMP6) induced cell death by a series of experiments, including morphological observation, ..
  2. Strack B, Calistri A, Craig S, Popova E, Gottlinger H. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689-99 pubmed
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  3. Morita E, Colf L, Karren M, Sandrin V, Rodesch C, Sundquist W. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010;107:12889-94 pubmed publisher
  4. Langelier C, von Schwedler U, Fisher R, De Domenico I, White P, Hill C, et al. Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol. 2006;80:9465-80 pubmed
    ..domain, both EAP45 and EAP30 bound the C-terminal domain of TSG101/ESCRT-I, and EAP20 bound the N-terminal half of CHMP6/ESCRT-III...
  5. Howard T, Stauffer D, Degnin C, Hollenberg S. CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci. 2001;114:2395-404 pubmed
    ..These observations identify the conserved CHMP/Chmp family as a set of proteins fundamental to understanding multivesicular body sorting in eukaryotic organisms. ..
  6. Im Y, Wollert T, Boura E, Hurley J. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell. 2009;17:234-43 pubmed publisher
    ..mapped to the second winged helix domain of human ESCRT-II subunit VPS25 and the first helix of ESCRT-III subunit VPS20. The crystal structure of this complex was determined at 2.0 A resolution...
  7. von Schwedler U, Stuchell M, Muller B, Ward D, Chung H, Morita E, et al. The protein network of HIV budding. Cell. 2003;114:701-13 pubmed
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses. ..
  8. Kieffer C, Skalicky J, Morita E, De Domenico I, Ward D, Kaplan J, et al. Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding. Dev Cell. 2008;15:62-73 pubmed publisher
    ..The solution structure of the VPS4 MIT-CHMP6 MIM2 complex revealed that MIM2 elements bind in extended conformations along the groove between the first and ..
  9. Richter C, West M, Odorizzi G. Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III. J Cell Sci. 2013;126:1881-90 pubmed publisher have the α-helical structure common to MIT domains, and it binds directly to a MIM1-like sequence in the Vps20 subunit of ESCRT-III...

More Information


  1. Peñalva M, Lucena Agell D, Arst H. Liaison alcaline: Pals entice non-endosomal ESCRTs to the plasma membrane for pH signaling. Curr Opin Microbiol. 2014;22:49-59 pubmed publisher
    ..necessary, Pal proteins are insufficient to transmit the signal, which additionally requires ESCRT-I, II and Vps20 with Snf7 in ESCRT-III...
  2. Zheng Z, Chang E. A bimolecular fluorescent complementation screen reveals complex roles of endosomes in Ras-mediated signaling. Methods Enzymol. 2014;535:25-38 pubmed publisher
    ..interactions and reveal where the binding takes place in live cells, we have identified three effectors, Cdc42, CHMP6, and VPS4A that interact with Ras proteins in endosomes...
  3. Zhao Y, Du J, Xiong B, Xu H, Jiang L. ESCRT components regulate the expression of the ER/Golgi calcium pump gene PMR1 through the Rim101/Nrg1 pathway in budding yeast. J Mol Cell Biol. 2013;5:336-44 pubmed publisher
    ..Here we show that deletion mutation of ESCRT components Snf7, Snf8, Stp22, Vps20, Vps25, Vps28, or Vps36 activates the calcium/calcineurin signaling in yeast cells, but surprisingly leads to a ..
  4. Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
    ..In Saccharomyces cerevisiae, ESCRT-III consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their ..
  5. Shim S, Merrill S, Hanson P. Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly. Mol Biol Cell. 2008;19:2661-72 pubmed publisher
    ..additional ESCRT-III proteins including CHMP1B, CHMP2A/hVps2-1, and CHMP3/hVps24 but not CHMP4A/hSnf7-1 or CHMP6/hVps20. LIP5 binds to a different region within CHMP5 than within the other ESCRT-III proteins...
  6. Christ L, Wenzel E, Liestøl K, Raiborg C, Campsteijn C, Stenmark H. ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission. J Cell Biol. 2016;212:499-513 pubmed publisher
    ..Here, we show that ESCRT-II and the ESCRT-II-binding ESCRT-III subunit CHMP6 cooperate with ESCRT-I to recruit CHMP4B, with ALIX providing a parallel recruitment arm...
  7. Lee I, Kai H, Carlson L, Groves J, Hurley J. Negative membrane curvature catalyzes nucleation of endosomal sorting complex required for transport (ESCRT)-III assembly. Proc Natl Acad Sci U S A. 2015;112:15892-7 pubmed publisher
    ..ESCRT-II and CHMP6 accelerate CHMP4B assembly by increasing the concentration of nucleation seeds...
  8. Bartusch C, Prange R. ESCRT Requirements for Murine Leukemia Virus Release. Viruses. 2016;8:103 pubmed publisher
    ..In contrast, neither the CHMP2B and CHMP4A isoforms nor CHMP3, CHMP5, and CHMP6 were found to be essential...
  9. Tang S, Buchkovich N, Henne W, Banjade S, Kim Y, Emr S. ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis. elife. 2016;5: pubmed publisher
    ..core domain and the C-terminus ?6 helix through two parallel ubiquitin-dependent pathways: the ESCRT-I-ESCRT-II-Vps20 pathway and the ESCRT-0-Bro1 pathway...
  10. Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed publisher
    ..However, depletion of proteins of the ESCRT-II complex or of the ESCRT-III protein, VPS20 (vacuolar protein sorting 20)/CHMP6 (charged MVB protein 6), failed to prevent the loss of MHC class I from the ..
  11. Kojima R, Obita T, Onoue K, Mizuguchi M. Structural Fine-Tuning of MIT-Interacting Motif 2 (MIM2) and Allosteric Regulation of ESCRT-III by Vps4 in Yeast. J Mol Biol. 2016;428:2392-2404 pubmed publisher
    ..Here, we have determined the crystal structure of Vps4-MIT in a complex with Vps20, a member of ESCRT-III, and revealed that Vps20 adopts a unique MIM2 conformation...
  12. Johnson N, West M, Odorizzi G. Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin hydrolase. Mol Biol Cell. 2017;28:661-672 pubmed publisher
    ..We further show that deubiquitination of ILV cargoes is inhibited via Doa4 binding to Vps20, which is the subunit of ESCRT-III that initiates assembly of the complex...
  13. Bowers K, Piper S, Edeling M, Gray S, Owen D, Lehner P, et al. Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII. J Biol Chem. 2006;281:5094-105 pubmed
    ..Our data suggest that mammalian ESCRTII may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body. ..
  14. Martin Serrano J, Yarovoy A, Perez Caballero D, Bieniasz P, Yaravoy A. Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc Natl Acad Sci U S A. 2003;100:12414-9 pubmed
  15. Pincetic A, Medina G, Carter C, Leis J. Avian sarcoma virus and human immunodeficiency virus, type 1 use different subsets of ESCRT proteins to facilitate the budding process. J Biol Chem. 2008;283:29822-30 pubmed publisher
    ..were more effective in rescue of Gag/Deltap2b budding than the ESCRT-II factor Eap20 or the ESCRT-III component CHMP6. Thus ESCRT components can substitute for Nedd4 family members in ASV Gag release...
  16. Kuang Z, Seo E, Leis J. Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15. J Virol. 2011;85:7153-61 pubmed publisher
    ..Upon ISGylation of each, interaction with LIP5 is no longer detected. Two other ESCRT-III proteins, CHMP4B and CHMP6, are also conjugated to ISG15...
  17. Weiss E, Gottlinger H. The role of cellular factors in promoting HIV budding. J Mol Biol. 2011;410:525-33 pubmed publisher
    ..Surprisingly, HIV-1 requires only a subset of ESCRT-III components, indicating that the membrane fission reaction that occurs during HIV-1 budding differs in crucial aspects from topologically related cellular abscission events. ..
  18. Schuh A, Hanna M, Quinney K, Wang L, Sarkeshik A, Yates J, et al. The VPS-20 subunit of the endosomal sorting complex ESCRT-III exhibits an open conformation in the absence of upstream activation. Biochem J. 2015;466:625-37 pubmed publisher
    ..Previous work suggests that Vps20 isoforms, like other ESCRT-III subunits, exhibits an auto-inhibited closed conformation in solution and its ..
  19. Cashikar A, Shim S, Roth R, Maldazys M, Heuser J, Hanson P. Structure of cellular ESCRT-III spirals and their relationship to HIV budding. elife. 2014;3: pubmed publisher
    ..Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral...
  20. Baietti M, Zhang Z, Mortier E, Melchior A, Degeest G, Geeraerts A, et al. Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012;14:677-85 pubmed publisher
    ..This study identifies a key role for syndecan-syntenin-ALIX in membrane transport and signalling processes. ..
  21. Peck J, Bowden E, Burbelo P. Structure and function of human Vps20 and Snf7 proteins. Biochem J. 2004;377:693-700 pubmed
    ..of yeast Snf7p, designated hSnf7-1, hSnf7-2 and hSnf7-3, and a single human Vps20p homologue, designated hVps20, that may have similar roles in humans...
  22. Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, et al. Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting. Biochem J. 2005;387:17-26 pubmed
    CHMP6 (charged multivesicular body protein 6) is a human orthologue of yeast Vps (vacuolar protein sorting) 20, a component of ESCRT (endosomal sorting complex required for transport)-III...
  23. Kumar B, Dutta D, Iqbal J, Ansari M, Roy A, Chikoti L, et al. ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016;12:e1005960 pubmed publisher
    ..molecules, as well as with upstream and downstream ESCRT components such as Hrs (ESCRT-0), EAP45 (ESCRT-II), CHMP6 (ESCRT-III) and CHMP5 (ESCRT-III) in the KSHV infected cells...
  24. Xia H, Shao D, Liu X, Wang Q, Zhou Y, Chen K. Identification and Characterization of BmVta1, a Bombyx mori (Lepidoptera: Bombycidae) Homologue for Vta1 That is Up-Regulated in Development. J Insect Sci. 2017;17: pubmed publisher
    b>Vps20-associated 1 (Vta1) positively regulates Vacuolar protein sorting 4 (Vps4) to disassemble endosomal sorting complex required for transport III (ESCRT-III) for repeated uses in multivesicular body (MVB) pathway, virus budding and ..
  25. Yue Q, Yu Q, Yang Q, Xu Y, Guo Y, Blissard G, et al. Distinct Roles of Cellular ESCRT-I and ESCRT-III Proteins in Efficient Entry and Egress of Budded Virions of Autographa californica Multiple Nucleopolyhedrovirus. J Virol. 2018;92: pubmed publisher
    ..In this study, the core components of ESCRT-I (Tsg101 and Vps28) and ESCRT-III (Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral complementation system ..
  26. Zheng Z, Cheng C, Fu X, Chen L, Xu L, Terrillon S, et al. CHMP6 and VPS4A mediate the recycling of Ras to the plasma membrane to promote growth factor signaling. Oncogene. 2012;31:4630-8 pubmed publisher
    ..Many isolated proteins regulate trafficking involving endosomes; CHMP6/VPS20 and VPS4A, which interact with ESCRT-III (Endosomal Sorting Complex Required for Transport-III), were chosen for ..
  27. Horii M, Shibata H, Kobayashi R, Katoh K, Yorikawa C, Yasuda J, et al. CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway. Biochem J. 2006;400:23-32 pubmed
    ..Among the ten CHMP proteins classified previously in six subfamilies (CHMP1-CHMP6), the C-terminal SNF7 domain of CHMP7 is most similar to the SNF7 domain of CHMP6, which associates with CHMP4 ..
  28. Goliand I, Nachmias D, Gershony O, Elia N. Inhibition of ESCRT-II-CHMP6 interactions impedes cytokinetic abscission and leads to cell death. Mol Biol Cell. 2014;25:3740-8 pubmed publisher
    ..Here we revisit the role of the upstream canonical ESCRT players ESCRT-II and CHMP6 in abscission...