CHMP4B

Summary

Gene Symbol: CHMP4B
Description: charged multivesicular body protein 4B
Alias: C20orf178, CHMP4A, CTPP3, CTRCT31, SNF7, SNF7-2, Shax1, VPS32B, Vps32-2, dJ553F4.4, charged multivesicular body protein 4b, SNF7 homolog associated with Alix 1, Snf7 homologue associated with Alix 1, chromatin modifying protein 4B, chromatin-modifying protein 4b, hSnf7-2, hVps32-2, vacuolar protein-sorting-associated protein 32-2
Species: human
Products:     CHMP4B

Top Publications

  1. Katoh K, Shibata H, Suzuki H, Nara A, Ishidoh K, Kominami E, et al. The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 2003;278:39104-13 pubmed
    ..the C-terminal region) by using a yeast two-hybrid screen, and we identified two similar human proteins, CHMP4a and CHMP4b (chromatin-modifying protein; charged multivesicular body protein), as novel binding partners of Alix...
  2. Bleck M, Itano M, Johnson D, Thomas V, North A, Bieniasz P, et al. Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding. Proc Natl Acad Sci U S A. 2014;111:12211-6 pubmed publisher
    ..protein Tsg101 is recruited together with Gag to the sites of HIV-1 assembly, whereas later-acting ESCRT proteins (Chmp4b and Vps4A) are recruited sequentially, once Gag assembly is completed...
  3. Zhou X, Pan S, Sun L, Corvera J, Lee Y, Lin S, et al. The CHMP4b- and Src-docking sites in the Bro1 domain are autoinhibited in the native state of Alix. Biochem J. 2009;418:277-84 pubmed publisher
    ..for transport)-linked processes, contains the docking sites for the ESCRT-III component CHMP4b (charged multivesicular body protein 4b) and the regulatory tyrosine kinase, Src...
  4. Carlton J, Agromayor M, Martin Serrano J. Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release. Proc Natl Acad Sci U S A. 2008;105:10541-6 pubmed publisher
    ..Last, we demonstrate that overexpression of Chmp4b and Chmp4c differentially inhibits HIV-1 release and cytokinesis, suggesting possible reasons for gene expansion ..
  5. Carlson L, Hurley J. In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters. Proc Natl Acad Sci U S A. 2012;109:16928-33 pubmed publisher
    ..These findings define the minimal sets of components needed to complete ESCRT assembly at HIV-1 budding sites, and provide a starting point for in vitro structural and biophysical dissection of the system. ..
  6. Sagona A, Nezis I, Pedersen N, Liestøl K, Poulton J, Rusten T, et al. PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody. Nat Cell Biol. 2010;12:362-71 pubmed publisher
    ..recruits a centrosomal protein, FYVE-CENT (ZFYVE26), and its binding partner TTC19, which in turn interacts with CHMP4B, an endosomal sorting complex required for transport (ESCRT)-III subunit implicated in the abscission step of ..
  7. McCullough J, Fisher R, Whitby F, Sundquist W, Hill C. ALIX-CHMP4 interactions in the human ESCRT pathway. Proc Natl Acad Sci U S A. 2008;105:7687-91 pubmed publisher
    ..we report that the Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C)...
  8. Zhou X, Si J, Corvera J, Gallick G, Kuang J. Decoding the intrinsic mechanism that prohibits ALIX interaction with ESCRT and viral proteins. Biochem J. 2010;432:525-34 pubmed publisher
    ..These findings predict that relieving the autoinhibitory intramolecular interaction of ALIX is a critical step for ALIX to participate in retroviral budding. ..
  9. Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, et al. Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting. Biochem J. 2005;387:17-26 pubmed
    ..Interactions of CHMP6 with another ESCRT-III component CHMP4b/Shax [Snf7 (sucrose non-fermenting 7) homologue associated with Alix] 1, one of three paralogues of human Vps32/..

More Information

Publications98

  1. Strack B, Calistri A, Craig S, Popova E, Gottlinger H. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689-99 pubmed
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  2. Van Engelenburg S, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan S, et al. Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits. Science. 2014;343:653-6 pubmed publisher
    ..of budding virions and released particles, with head-localized levels of CHMP2A decreasing relative to Tsg101 and CHMP4B upon virus abscission...
  3. Effantin G, Dordor A, Sandrin V, Martinelli N, Sundquist W, Schoehn G, et al. ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding. Cell Microbiol. 2013;15:213-26 pubmed publisher
    ..This is consistent with surface plasmon resonance affinity measurements that suggest sequential CHMP4B-CHMP3-CHMP2A recruitment while showing that both CHMP2A and CHMP2B interact with CHMP4B, in agreement with their ..
  4. von Schwedler U, Stuchell M, Muller B, Ward D, Chung H, Morita E, et al. The protein network of HIV budding. Cell. 2003;114:701-13 pubmed
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses. ..
  5. Popov S, Popova E, Inoue M, Gottlinger H. Divergent Bro1 domains share the capacity to bind human immunodeficiency virus type 1 nucleocapsid and to enhance virus-like particle production. J Virol. 2009;83:7185-93 pubmed publisher
    ..Unexpectedly, Brox retained significant activity even if its CHMP4 binding site was disrupted. Thus, the ability to assist in VLP production may be an intrinsic property of the boomerang-shaped Bro1 domain. ..
  6. Pires R, Hartlieb B, Signor L, Schoehn G, Lata S, Roessle M, et al. A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filaments. Structure. 2009;17:843-56 pubmed publisher
    ..ALIX dimerizes in vivo and dimeric ALIX colocalizes with CHMP4B upon coexpression. We show further that ALIX dimerization affects HIV-1 budding...
  7. Martinelli N, Hartlieb B, Usami Y, Sabin C, Dordor A, Miguet N, et al. CC2D1A is a regulator of ESCRT-III CHMP4B. J Mol Biol. 2012;419:75-88 pubmed publisher
    ..Here, we show that CC2D1A is a novel regulator of ESCRT-III CHMP4B function...
  8. Usami Y, Popov S, Weiss E, Vriesema Magnuson C, Calistri A, Gottlinger H. Regulation of CHMP4/ESCRT-III function in human immunodeficiency virus type 1 budding by CC2D1A. J Virol. 2012;86:3746-56 pubmed publisher
    ..Both proteins bind to the core domain of CHMP4B, which has a strong propensity to polymerize and to inhibit HIV-1 budding...
  9. Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P, et al. The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release. Structure. 2011;19:1485-95 pubmed publisher
    ..These studies identify a new functional determinant in the Bro1 domain of Alix. ..
  10. Shiels A, Bennett T, Knopf H, Yamada K, Yoshiura K, Niikawa N, et al. CHMP4B, a novel gene for autosomal dominant cataracts linked to chromosome 20q. Am J Hum Genet. 2007;81:596-606 pubmed
    ..386A-->T) in exon 3 of the gene for chromatin modifying protein-4B (CHMP4B) that was predicted to result in the nonconservative substitution of a valine residue for a phylogenetically ..
  11. Jouvenet N, Zhadina M, Bieniasz P, Simon S. Dynamics of ESCRT protein recruitment during retroviral assembly. Nat Cell Biol. 2011;13:394-401 pubmed publisher
  12. Morita E, Colf L, Karren M, Sandrin V, Rodesch C, Sundquist W. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010;107:12889-94 pubmed publisher
  13. Ichioka F, Kobayashi R, Katoh K, Shibata H, Maki M. Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4). FEBS J. 2008;275:682-92 pubmed publisher
    ..and Strep-tagged farnesylation-defective mutant (Cys-->Ser mutation; Strep-Brox(C408S)) pulled down FLAG-tagged CHMP4b that was coexpressed in HEK293 cells...
  14. Katoh K, Shibata H, Hatta K, Maki M. CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms. Arch Biochem Biophys. 2004;421:159-65 pubmed
    ..In addition to the previously isolated cDNAs for two CHMP4 proteins (CHMP4a and CHMP4b), we isolated a cDNA for a new member of the CHMP4 subfamily (designated CHMP4c)...
  15. Peck J, Bowden E, Burbelo P. Structure and function of human Vps20 and Snf7 proteins. Biochem J. 2004;377:693-700 pubmed
    ..overexpressed hVps20 showed a typical endosomal membrane-staining pattern, and co-expression of hVps20 with Snf7-1 dispersed the large Snf7-staining vesicles...
  16. Martin Serrano J, Yarovoy A, Perez Caballero D, Bieniasz P, Yaravoy A. Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc Natl Acad Sci U S A. 2003;100:12414-9 pubmed
  17. Guizetti J, Schermelleh L, Mäntler J, Maar S, Poser I, Leonhardt H, et al. Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science. 2011;331:1616-20 pubmed publisher
    ..The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures. ..
  18. Usami Y, Popov S, Gottlinger H. Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site. J Virol. 2007;81:6614-22 pubmed
    ..One of these patches mediates the interaction between ALIX and the ESCRT-III component CHMP4B, and mutations which disrupt the interaction also abolish the activity of ALIX in viral budding...
  19. Hanson P, Roth R, Lin Y, Heuser J. Plasma membrane deformation by circular arrays of ESCRT-III protein filaments. J Cell Biol. 2008;180:389-402 pubmed publisher
    ..electron microscopy to examine polymers formed by the ESCRT-III proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B). When overexpressed, these proteins target to endosomes and the plasma membrane...
  20. Robijns J, Molenberghs F, Sieprath T, Corne T, Verschuuren M, De Vos W. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. 2016;6:30325 pubmed publisher
    ..by the severity of the rupture event, and could be prolonged by knockdown of the ESCRT-III complex component CHMP4B. In conclusion, our approach reveals regulators of nuclear rupture induction and repair, which may have critical ..
  21. Lone M, Kungl T, Koper A, Bottenberg W, KAMMERER R, Klein M, et al. The nuclear protein Waharan is required for endosomal-lysosomal trafficking in Drosophila. J Cell Sci. 2010;123:2369-74 pubmed publisher
    ..data demonstrating that Wah acts earlier in the trafficking pathway than the Escrt-III component Drosophila Shrb (snf7 in Schizosaccharomyces pombe), our results indicate that Wah is essential for endocytic trafficking at the late ..
  22. Kogan V, Millstein J, London S, Ober C, White S, Naureckas E, et al. Genetic-Epigenetic Interactions in Asthma Revealed by a Genome-Wide Gene-Centric Search. Hum Hered. 2018;83:130-152 pubmed publisher
    ..Twelve genes, PF4, ATF3, TPRA1, HOPX, SCARNA18, STC1, OR10K1, UPK1B, LOC101928523, LHX6, CHMP4B, and LANCL1, exhibited statistically significant SNP-CpG interactions (false discovery rate = 0.05)...
  23. Peñalva M, Lucena Agell D, Arst H. Liaison alcaline: Pals entice non-endosomal ESCRTs to the plasma membrane for pH signaling. Curr Opin Microbiol. 2014;22:49-59 pubmed publisher
    ..Pal proteins are insufficient to transmit the signal, which additionally requires ESCRT-I, II and Vps20 with Snf7 in ESCRT-III...
  24. Webster B, Colombi P, Jäger J, Lusk C. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell. 2014;159:388-401 pubmed publisher
    ..Heh2 recruits the endosomal sorting complex required for transport (ESCRT)-III subunit Snf7 and the AAA-ATPase Vps4 to destabilize and clear defective NPC assembly intermediates...
  25. Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, et al. The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling. PLoS Genet. 2015;11:e1005749 pubmed publisher
    ..We could show that CC2D1A and its interaction partner CHMP4B are localised on endosomes in MEF cells, when the activity of the endosomal protein VPS4 is reduced...
  26. Shim S, Merrill S, Hanson P. Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly. Mol Biol Cell. 2008;19:2661-72 pubmed publisher
    ..that it binds well to additional ESCRT-III proteins including CHMP1B, CHMP2A/hVps2-1, and CHMP3/hVps24 but not CHMP4A/hSnf7-1 or CHMP6/hVps20. LIP5 binds to a different region within CHMP5 than within the other ESCRT-III proteins...
  27. Wolters N, Amerik A. The N-terminal domains determine cellular localization and functions of the Doa4 and Ubp5 deubiquitinating enzymes. Biochem Biophys Res Commun. 2015;467:570-6 pubmed publisher
    ..Here we show that Doa4 physically interacts with the ESCRT-III component Snf7 and preferentially cleaves Lys63-linked ubiquitin oligomers involved in membrane protein trafficking...
  28. Singh S, Gupta M, Pandher S, Kaur G, Goel N, Rathore P, et al. RNA sequencing, selection of reference genes and demonstration of feeding RNAi in Thrips tabaci (Lind.) (Thysanoptera: Thripidae). BMC Mol Biol. 2019;20:6 pubmed publisher
    ..Feeding dsSNF7 and dsAQP caused 16.4- and 14.47-fold reduction in SNF7 and AQP mRNA levels respectively, when compared to their levels in dsGFP fed control insects...
  29. Chiaruttini N, Redondo Morata L, Colom A, Humbert F, Lenz M, Scheuring S, et al. Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation. Cell. 2015;163:866-79 pubmed publisher
    ..However, how ESCRT-III polymerization generates membrane curvature remains debated. Here, we show that Snf7, the main component of ESCRT-III, polymerizes into spirals at the surface of lipid bilayers...
  30. Terrell A, Anand D, Smith S, Dang C, Waters S, Pathania M, et al. Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes. Exp Eye Res. 2015;131:42-55 pubmed publisher
    ..Additionally, 21EM15 microarrays indicate expression of Chmp4b, Cryab and Tcfap2a among others important genes...
  31. Schöneberg J, Pavlin M, Yan S, Righini M, Lee I, Carlson L, et al. ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science. 2018;362:1423-1428 pubmed publisher
    ..We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane ..
  32. Shen J, Liu Y, Song Y, Li L, Duan C, Zhou Y, et al. CHMP4B, ESCRT-III associating protein, associated with neuronal apoptosis following intracerebral hemorrhage. Brain Res. 2015;1597:1-13 pubmed publisher
    ..b>CHMP4B, a core component of the endosomal sorting complex required for transport (ESCRT)-III, is requisite for endosomal ..
  33. Shen Q, Schuh A, Zheng Y, Quinney K, Wang L, Hanna M, et al. Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly. J Cell Biol. 2014;206:763-77 pubmed publisher
    ..Using cryogenic electron microscopy (cryo-EM), we found that the full-length ESCRT-III subunit Vps32/CHMP4B spontaneously forms single-stranded spiral filaments...
  34. Zhao Y, Du J, Xiong B, Xu H, Jiang L. ESCRT components regulate the expression of the ER/Golgi calcium pump gene PMR1 through the Rim101/Nrg1 pathway in budding yeast. J Mol Cell Biol. 2013;5:336-44 pubmed publisher
    ..Here we show that deletion mutation of ESCRT components Snf7, Snf8, Stp22, Vps20, Vps25, Vps28, or Vps36 activates the calcium/calcineurin signaling in yeast cells, but ..
  35. Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
    ..In Saccharomyces cerevisiae, ESCRT-III consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their ..
  36. Curwin A, Brouwers N, Alonso Y Adell M, Teis D, Turacchio G, Parashuraman S, et al. ESCRT-III drives the final stages of CUPS maturation for unconventional protein secretion. elife. 2016;5: pubmed publisher
    ..The major ESCRT-III subunit Snf7 localizes transiently to CUPS and this was accelerated in vps4? cells, correlating with increased Acb1 secretion...
  37. Christiaens O, Prentice K, Pertry I, Ghislain M, Bailey A, Niblett C, et al. RNA interference: a promising biopesticide strategy against the African Sweetpotato Weevil Cylas brunneus. Sci Rep. 2016;6:38836 pubmed publisher
    ..The three best performing dsRNAs, targeting pros?2, rps13 and the homolog of Diabrotica virgifera snf7, were then used in further feeding trials to investigate RNAi by oral delivery...
  38. Bartusch C, Prange R. ESCRT Requirements for Murine Leukemia Virus Release. Viruses. 2016;8:103 pubmed publisher
    ..the effects of individual ESCRT-III knockdowns, VLP and virion release was profoundly inhibited in CHMP2A- and CHMP4B-knockdown cells...
  39. Vietri M, Schink K, Campsteijn C, Wegner C, Schultz S, Christ L, et al. Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing. Nature. 2015;522:231-5 pubmed publisher
  40. Buono R, Leier A, Paez Valencia J, Pennington J, Goodman K, Miller N, et al. ESCRT-mediated vesicle concatenation in plant endosomes. J Cell Biol. 2017;216:2167-2177 pubmed publisher
    ..Consistent with a potential role as a diffusion barrier, we find that the ESCRT-III protein SNF7 remains associated with ILVs and is delivered to the vacuole for degradation.
  41. Webster B, Thaller D, Jager J, Ochmann S, Borah S, Lusk C. Chm7 and Heh1 collaborate to link nuclear pore complex quality control with nuclear envelope sealing. EMBO J. 2016;35:2447-2467 pubmed
    ..We establish direct binding between Heh2 and the "open" forms of both Chm7 and the ESCRT-III, Snf7, and between Chm7 and Snf7...
  42. Urquhart W, Mueller G, Carleton S, Song Z, Perez T, Uffman J, et al. A novel method of demonstrating the molecular and functional equivalence between in vitro and plant-produced double-stranded RNA. Regul Toxicol Pharmacol. 2015;73:607-12 pubmed publisher
    ..results in the formation of a double-stranded RNA (dsRNA) transcript containing a 240 bp fragment of the WCR Snf7 gene (DvSnf7) that confers resistance to corn rootworm by suppressing levels of DvSnf7 mRNA in WCR after root ..
  43. Cardona López X, Cuyas L, Marín E, Rajulu C, Irigoyen M, Gil E, et al. ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis. Plant Cell. 2015;27:2560-81 pubmed publisher
    ..Here, we describe ALIX, a cytosolic protein that associates with MVB by interacting with ESCRT-III subunit SNF7 and mediates PHT1;1 trafficking to the vacuole in Arabidopsis thaliana...
  44. Livramento K, Freitas N, Máximo W, Zanetti R, Paiva L. Gene Expression Profile Analysis is Directly Affected by the Selected Reference Gene: The Case of Leaf-Cutting Atta Sexdens. Insects. 2018;9: pubmed publisher
    ..The SNF7 gene expression pattern was stable in all evaluated sample set...
  45. Petrick J, Frierdich G, Carleton S, Kessenich C, Silvanovich A, Zhang Y, et al. Corn rootworm-active RNA DvSnf7: Repeat dose oral toxicology assessment in support of human and mammalian safety. Regul Toxicol Pharmacol. 2016;81:57-68 pubmed publisher
    ..base pair (bp) dsRNA fragment targeting for suppression the western corn rootworm (Diabrotica virgifera virgifera) Snf7 gene (DvSnf7)...
  46. Tang S, Buchkovich N, Henne W, Banjade S, Kim Y, Emr S. ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis. elife. 2016;5: pubmed publisher
    ..Previously, we determined X-ray crystal structures of ESCRT-III subunit Snf7, the yeast CHMP4 ortholog, in its active and polymeric state (Tang et al., 2015)...
  47. McMillan B, Tibbe C, Drabek A, Seegar T, Blacklow S, Klein T. Structural Basis for Regulation of ESCRT-III Complexes by Lgd. Cell Rep. 2017;19:1750-1757 pubmed publisher
    ..complex induces outward membrane budding and fission through homotypic polymerization of its core component Shrub/CHMP4B. Shrub activity is regulated by its direct interaction with a protein called Lgd in flies, or CC2D1A or B in ..
  48. Dema A, Macaluso F, Sgrò F, Berto G, Bianchi F, Chiotto A, et al. Citron kinase-dependent F-actin maintenance at midbody secondary ingression sites mediates abscission. J Cell Sci. 2018;131: pubmed publisher
    ..midbody severing is mediated by formation of secondary midbody ingression sites, where the ESCRT III component CHMP4B is recruited to mediate membrane fusion...
  49. Kamranvar S, Gupta D, Huang Y, Gupta R, Johansson S. Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody. Oncotarget. 2016;7:30820-30 pubmed publisher
    ..fibroblasts, but it was unable to efficiently recruit ALIX, TSG101, and consequently the ESCRT-III subunit CHMP4B was missing in the non-adherent cells...
  50. Mamińska A, Bartosik A, Banach Orłowska M, Pilecka I, Jastrzębski K, Zdżalik Bielecka D, et al. ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors. Sci Signal. 2016;9:ra8 pubmed publisher
    ..which mediate receptor trafficking and degradation, and found that the components Tsg101, Vps28, UBAP1, and CHMP4B were essential to restrict constitutive NF-κB signaling in human embryonic kidney 293 cells...
  51. Lee I, Kai H, Carlson L, Groves J, Hurley J. Negative membrane curvature catalyzes nucleation of endosomal sorting complex required for transport (ESCRT)-III assembly. Proc Natl Acad Sci U S A. 2015;112:15892-7 pubmed publisher
    ..The assembly of the core ESCRT-III subunit CHMP4B/Snf7 is preferentially nucleated in the resulting 100-nm-deep membrane concavities...
  52. Tang S, Henne W, Borbat P, Buchkovich N, Freed J, Mao Y, et al. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. elife. 2015;4: pubmed publisher
    ..Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation...
  53. Mogilicherla K, Howell J, Palli S. Improving RNAi in the Brown Marmorated Stink Bug: Identification of target genes and reference genes for RT-qPCR. Sci Rep. 2018;8:3720 pubmed publisher
    ..Feeding dsRNA targeting five of these genes (IAP, ATPase, SNF7, GPCR, and PPI) to nymphs caused more than 70% mortality by three of the five dsRNAs tested...
  54. Rodrigues J, Lydall D. Cis and trans interactions between genes encoding PAF1 complex and ESCRT machinery components in yeast. Curr Genet. 2018;64:1105-1116 pubmed publisher
    ..lethal with deletions of other components of the ESCRT-II (SNF8 and VPS25), ESCRT-I (STP22), or ESCRT-III (SNF7) complexes...
  55. Zhang Y, Li W, Chu M, Chen H, Yu H, Fang C, et al. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ. Mycopathologia. 2016;181:329-39 pubmed publisher
    ..shown that at neutral-to-alkaline pH, hyphal growth is dependent on the Rim101 pathway whose activation requires Snf7, a member of the ESCRT system. In this work, we described the purification and characterization of the C...
  56. Yue Q, Yu Q, Yang Q, Xu Y, Guo Y, Blissard G, et al. Distinct Roles of Cellular ESCRT-I and ESCRT-III Proteins in Efficient Entry and Egress of Budded Virions of Autographa californica Multiple Nucleopolyhedrovirus. J Virol. 2018;92: pubmed publisher
    ..In this study, the core components of ESCRT-I (Tsg101 and Vps28) and ESCRT-III (Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral complementation system and RNA ..
  57. Lee J, Liu L, Javier R, Kreitzer A, Delaloy C, Gao F. ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate transmembrane cargos in hESC-derived human neurons. Mol Brain. 2011;4:37 pubmed publisher
    ..Moreover, we found that among the three human paralogs of the yeast ESCRT-III subunit Snf7, hSnf7-1 and hSnf7-2 are most abundantly expressed in human neurons...
  58. Cashikar A, Shim S, Roth R, Maldazys M, Heuser J, Hanson P. Structure of cellular ESCRT-III spirals and their relationship to HIV budding. elife. 2014;3: pubmed publisher
    ..Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral...
  59. Ichioka F, Takaya E, Suzuki H, Kajigaya S, Buchman V, Shibata H, et al. HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions. Arch Biochem Biophys. 2007;457:142-9 pubmed
    ..In the yeast two-hybrid assay, HD-PTP showed positive interactions with CHMP4b/Shax1, TSG101, endophilin A1 and ALG-2 but not with either RabGAPLP or CIN85...
  60. Horii M, Shibata H, Kobayashi R, Katoh K, Yorikawa C, Yasuda J, et al. CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway. Biochem J. 2006;400:23-32 pubmed
    ..human embryonic kidney) cells that overexpressed Strep-tagged CHMP7 and GFP (green fluorescent protein)-fused CHMP4b (also named Shax1) revealed a positive interaction between the C-terminal half of CHMP7 and CHMP4b...
  61. Sette P, Nagashima K, Piper R, Bouamr F. Ubiquitin conjugation to Gag is essential for ESCRT-mediated HIV-1 budding. Retrovirology. 2013;10:79 pubmed publisher
    ..These studies demonstrate a necessary and natural role for ubiquitin in ESCRT-dependent viral release and indicate a critical role for ubiquitination of Gag rather than ubiquitination of ESCRTs themselves. ..
  62. Dores M, Lin H, J Grimsey N, Méndez F, Trejo J. The α-arrestin ARRDC3 mediates ALIX ubiquitination and G protein-coupled receptor lysosomal sorting. Mol Biol Cell. 2015;26:4660-73 pubmed publisher
    ..Depletion of ARRDC3 by small interfering RNA disrupts ALIX interaction with activated PAR1 and the CHMP4B ESCRT-III subunit, suggesting that ARRDC3 regulates ALIX activity...
  63. Lee J, Oh K, Lee D, Kim B, Choi J, Ku B, et al. Structural Study of the HD-PTP Bro1 Domain in a Complex with the Core Region of STAM2, a Subunit of ESCRT-0. PLoS ONE. 2016;11:e0149113 pubmed publisher
    ..and is associated with EGFR trafficking by interacting with the ESCRT-0 protein STAM2 and the ESCRT-III protein CHMP4B via its N-terminal Bro1 domain...
  64. Christ L, Wenzel E, Liestøl K, Raiborg C, Campsteijn C, Stenmark H. ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission. J Cell Biol. 2016;212:499-513 pubmed publisher
    ..The ESCRT-III subunit CHMP4B is a key effector in abscission, whereas its paralogue, CHMP4C, is a component in the abscission checkpoint that ..
  65. Sandrin V, Sundquist W. ESCRT requirements for EIAV budding. Retrovirology. 2013;10:104 pubmed publisher
    ..wild type and mutant constructs revealed budding requirements for the following ESCRT proteins: ALIX, CHMP4B, CHMP2A and VPS4A or VPS4B...
  66. Mierzwa B, Chiaruttini N, Redondo Morata L, von Filseck J, König J, Larios J, et al. Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nat Cell Biol. 2017;19:787-798 pubmed publisher
    ..In vitro, the Vps2/Vps24 subunits of ESCRT-III formed side-by-side filaments with Snf7 and inhibited further polymerization, but the growth inhibition was alleviated by the addition of Vps4 and ATP...
  67. Adell M, Migliano S, Upadhyayula S, Bykov Y, Sprenger S, Pakdel M, et al. Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding. elife. 2017;6: pubmed publisher
    ..During their 3-45 s lifetimes, the ESCRT-III assemblies accumulated 75-200 Snf7 and 15-50 Vps24 molecules. Productive budding events required at least two additional Vps4 hexamers...
  68. Dores M, Chen B, Lin H, Soh U, Paing M, Montagne W, et al. ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting. J Cell Biol. 2012;197:407-19 pubmed publisher
    ..This study reveals a novel MVB/lysosomal sorting pathway for signaling receptors that bypasses the requirement for ubiquitination and ubiquitin-binding ESCRTs and may be applicable to a subset of GPCRs containing YPX(n)L motifs. ..
  69. Carlson L, Shen Q, Pavlin M, Hurley J. ESCRT Filaments as Spiral Springs. Dev Cell. 2015;35:397-8 pubmed publisher
    ..2015) reveal the mechanical properties of the mysterious spiral filaments formed by the yeast ESCRT-III protein Snf7. The spirals are shown to be springs whose bending drives membrane deformation and perhaps membrane scission.
  70. Kuang Z, Seo E, Leis J. Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15. J Virol. 2011;85:7153-61 pubmed publisher
    ..Upon ISGylation of each, interaction with LIP5 is no longer detected. Two other ESCRT-III proteins, CHMP4B and CHMP6, are also conjugated to ISG15...
  71. Sagona A, Nezis I, Stenmark H. Association of CHMP4B and autophagy with micronuclei: implications for cataract formation. Biomed Res Int. 2014;2014:974393 pubmed publisher
    ..Here we show that the ESCRT-III subunit CHMP4B not only localizes to normal cytokinetic bridges but also to chromosome bridges and micronuclei, the latter ..
  72. Prescher J, Baumgärtel V, Ivanchenko S, Torrano A, Bräuchle C, Muller B, et al. Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites. PLoS Pathog. 2015;11:e1004677 pubmed publisher
    ..super-resolution fluorescence microscopy to elucidate the size and structure of the ESCRT components Tsg101, ALIX, CHMP4B and CHMP2A during HIV-1 budding below the diffraction limit...
  73. Doyotte A, Mironov A, McKenzie E, Woodman P. The Bro1-related protein HD-PTP/PTPN23 is required for endosomal cargo sorting and multivesicular body morphogenesis. Proc Natl Acad Sci U S A. 2008;105:6308-13 pubmed publisher
    ..Rescue experiments using an RNAi-resistant version of HD-PTP and HD-PTP mutants demonstrated an essential role for the HD-PTP Bro1 domain, with ESCRT-III binding correlating with full biological activity. ..
  74. Capalbo L, Montembault E, Takeda T, Bassi Z, Glover D, D Avino P. The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis. Open Biol. 2012;2:120070 pubmed publisher
    ..Here, we show that the CPC subunit Borealin interacts directly with the Snf7 components of ESCRT-III in both Drosophila and human cells...
  75. McMillan B, Tibbe C, Jeon H, Drabek A, Klein T, Blacklow S. Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein. Cell Rep. 2016;16:1211-1217 pubmed publisher
    ..The ESCRT-III protein Shrub in flies, or its homologs in yeast (Snf7) or humans (CHMP4B), is a critical polymerizing component of ESCRT-III needed to effect membrane fission...
  76. Olmos Y, Hodgson L, Mantell J, Verkade P, Carlton J. ESCRT-III controls nuclear envelope reformation. Nature. 2015;522:236-9 pubmed publisher
    ..component charged multivesicular body protein 2A (CHMP2A) is directed to the forming NE through binding to CHMP4B, and provides an activity essential for NE reformation...
  77. Deshar R, Cho E, Yoon S, Yoon J. CC2D1A and CC2D1B regulate degradation and signaling of EGFR and TLR4. Biochem Biophys Res Commun. 2016;480:280-287 pubmed publisher
    ..Here, we report that CC2D1A binds to CHMP4B polymers formed on endosomes to regulate the endosomal sorting pathway...
  78. Bendjennat M, Saffarian S. The Race against Protease Activation Defines the Role of ESCRTs in HIV Budding. PLoS Pathog. 2016;12:e1005657 pubmed publisher
    ..We highlight the sensitivity of HIV to budding "on-time" and suggest that budding delay is a potent mechanism for inhibition of infectious retroviral release. ..
  79. Sharma M, Pampinella F, Nemes C, Benharouga M, So J, Du K, et al. Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes. J Cell Biol. 2004;164:923-33 pubmed
  80. Metcalf D, Isaacs A. The role of ESCRT proteins in fusion events involving lysosomes, endosomes and autophagosomes. Biochem Soc Trans. 2010;38:1469-73 pubmed publisher
  81. Mu R, Dussupt V, Jiang J, Sette P, Rudd V, Chuenchor W, et al. Two distinct binding modes define the interaction of Brox with the C-terminal tails of CHMP5 and CHMP4B. Structure. 2012;20:887-98 pubmed publisher
    ..Here we demonstrate that hydrophobic residues at the CHMP4B C-terminal amphipathic α helix bind a concave surface of Brox, a mammalian paralog of Alix...
  82. Row P, Liu H, Hayes S, Welchman R, Charalabous P, Hofmann K, et al. The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degradation. J Biol Chem. 2007;282:30929-37 pubmed
  83. Jermy A. Virology: ESCRTing retroviruses to the exit. Nat Rev Microbiol. 2011;9:314 pubmed publisher
  84. Pereira A, Velez A, Meinke L, Siegfried B. Sublethal Effects of vATPase-A and Snf7 dsRNAs on Biology of Southern Corn Rootworm, Diabrotica undecimpunctata howardi Barber. J Econ Entomol. 2017;: pubmed publisher
    ..Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae), were exposed to the LC50 of vATPase-A and Snf7 double-stranded RNAs (dsRNAs), and the effects on female fecundity, egg viability, male fitness as measured by ..
  85. Ariumi Y, Kuroki M, Maki M, Ikeda M, Dansako H, Wakita T, et al. The ESCRT system is required for hepatitis C virus production. PLoS ONE. 2011;6:e14517 pubmed publisher
    ..susceptibility gene 101 (TSG101), apoptosis-linked gene 2 interacting protein X (Alix), Vps4B, charged multivesicular body protein 4b (CHMP4b), or Brox, all of which are components of the ESCRT system...
  86. Lu Y, Zhang Z, Sun D, Sweeney S, Gao F. Syntaxin 13, a genetic modifier of mutant CHMP2B in frontotemporal dementia, is required for autophagosome maturation. Mol Cell. 2013;52:264-71 pubmed publisher
    ..These results suggest that STX13 is a genetic modifier of ESCRT-III dysfunction and participates in the maturation of phagophores into closed autophagosomes. ..
  87. Ding X, Stewart M, Sharei A, Weaver J, Langer R, Jensen K. High-throughput Nuclear Delivery and Rapid Expression of DNA via Mechanical and Electrical Cell-Membrane Disruption. Nat Biomed Eng. 2017;1: pubmed publisher
    ..By tracking the localization of the ESCRT-III (endosomal sorting complexes required for transport) protein CHMP4B, we show that the integrity of the nuclear envelope is recovered within 15 minutes of treatment...
  88. Xu B, Fu Y, Liu Y, Agvanian S, Wirka R, Baum R, et al. The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles. PLoS Biol. 2017;15:e2002354 pubmed publisher
    ..membrane fission complex endosomal sorting complexes required for transport (ESCRT)-III subunit charged multivesicular body protein 4B (CHMP4B) colocalizes and coimmunoprecipitates with cBIN1, an interaction enhanced by actin ..
  89. Hu B, Jiang D, Chen Y, Wei L, Zhang S, Zhao F, et al. High CHMP4B expression is associated with accelerated cell proliferation and resistance to doxorubicin in hepatocellular carcinoma. Tumour Biol. 2015;36:2569-81 pubmed publisher
    b>Charged multivesicular body protein 4B (CHMP4B), a subunit of the endosomal sorting complex required for transport (ESCRT)-III complex, plays an important part in cytokinetic membrane abscission and the late stage of mitotic cell division...