CHMP4A

Summary

Gene Symbol: CHMP4A
Description: charged multivesicular body protein 4A
Alias: C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A, charged multivesicular body protein 4a, SNF7 homolog associated with Alix-2, Snf7 homologue associated with Alix 2, chromatin modifying protein 4A, vacuolar protein sorting-associated protein 32-1
Species: human
Products:     CHMP4A

Top Publications

  1. Usami Y, Popov S, Popova E, Inoue M, Weissenhorn W, G Göttlinger H. The ESCRT pathway and HIV-1 budding. Biochem Soc Trans. 2009;37:181-4 pubmed publisher
    ..This effect of Alix depends on its ability to interact with CHMP4, suggesting that it is the recruitment of CHMPs that ultimately drives virus release...
  2. Fisher R, Chung H, Zhai Q, Robinson H, Sundquist W, Hill C. Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding. Cell. 2007;128:841-52 pubmed
    ..YPX(n)L late domains bind in a conserved hydrophobic pocket on the second arm near the apex of the V, whereas CHMP4/ESCRT-III proteins bind a conserved hydrophobic patch on the Bro1 domain, and both interactions are required for ..
  3. Shim S, Kimpler L, Hanson P. Structure/function analysis of four core ESCRT-III proteins reveals common regulatory role for extreme C-terminal domain. Traffic. 2007;8:1068-79 pubmed
    ..Here, we compare four human ESCRT-III proteins - hVps2-1/CHMP2a, hVps24/CHMP3, hVps20/CHMP6, and hSnf7-1/CHMP4a - to each other, studying the effects of deleting predicted alpha-helical domains on their behavior in transfected ..
  4. Katoh K, Shibata H, Suzuki H, Nara A, Ishidoh K, Kominami E, et al. The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 2003;278:39104-13 pubmed
    ..C-terminal region) by using a yeast two-hybrid screen, and we identified two similar human proteins, CHMP4a and CHMP4b (chromatin-modifying protein; charged multivesicular body protein), as novel binding partners of Alix...
  5. Peck J, Bowden E, Burbelo P. Structure and function of human Vps20 and Snf7 proteins. Biochem J. 2004;377:693-700 pubmed
    ..overexpressed hVps20 showed a typical endosomal membrane-staining pattern, and co-expression of hVps20 with Snf7-1 dispersed the large Snf7-staining vesicles...
  6. Strack B, Calistri A, Craig S, Popova E, Gottlinger H. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689-99 pubmed
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  7. Hanson P, Roth R, Lin Y, Heuser J. Plasma membrane deformation by circular arrays of ESCRT-III protein filaments. J Cell Biol. 2008;180:389-402 pubmed publisher
    ..electron microscopy to examine polymers formed by the ESCRT-III proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B). When overexpressed, these proteins target to endosomes and the plasma membrane...
  8. Lin Y, Kimpler L, Naismith T, Lauer J, Hanson P. Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1. J Biol Chem. 2005;280:12799-809 pubmed
    ..Together with earlier studies, our work suggests that a variety of ESCRT-III-containing polymers can assemble on membranes and recruit SKD1 during formation of the MVB. ..
  9. Sundquist W, Kräusslich H. HIV-1 assembly, budding, and maturation. Cold Spring Harb Perspect Med. 2012;2:a006924 pubmed publisher
    ..Here, we review our current understanding of the mechanisms of HIV-1 assembly, budding, and maturation, starting with a general overview and then providing detailed descriptions of each of the different stages of virion morphogenesis. ..

More Information

Publications98

  1. McCullough J, Fisher R, Whitby F, Sundquist W, Hill C. ALIX-CHMP4 interactions in the human ESCRT pathway. Proc Natl Acad Sci U S A. 2008;105:7687-91 pubmed publisher
    ..we report that the Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C)...
  2. Weiss E, Gottlinger H. The role of cellular factors in promoting HIV budding. J Mol Biol. 2011;410:525-33 pubmed publisher
    ..Surprisingly, HIV-1 requires only a subset of ESCRT-III components, indicating that the membrane fission reaction that occurs during HIV-1 budding differs in crucial aspects from topologically related cellular abscission events. ..
  3. Morita E, Colf L, Karren M, Sandrin V, Rodesch C, Sundquist W. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010;107:12889-94 pubmed publisher
  4. von Schwedler U, Stuchell M, Muller B, Ward D, Chung H, Morita E, et al. The protein network of HIV budding. Cell. 2003;114:701-13 pubmed
    ..interactions, with AIP1 playing a key role in linking complexes that act early (TSG101/ESCRT-I) and late (CHMP4/ESCRT-III) in the pathway...
  5. Martin Serrano J, Yarovoy A, Perez Caballero D, Bieniasz P, Yaravoy A. Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc Natl Acad Sci U S A. 2003;100:12414-9 pubmed
  6. Katoh K, Shibata H, Hatta K, Maki M. CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms. Arch Biochem Biophys. 2004;421:159-65 pubmed
    The ALG-2-interacting protein Alix has recently been demonstrated to associate with CHMP4b that is a human homologue of yeast Snf7p (also named Vps32p) and a member of the family of small coiled-coil proteins named CHMP implicated in ..
  7. Carlton J, Agromayor M, Martin Serrano J. Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release. Proc Natl Acad Sci U S A. 2008;105:10541-6 pubmed publisher
    ..Last, we demonstrate that overexpression of Chmp4b and Chmp4c differentially inhibits HIV-1 release and cytokinesis, suggesting possible reasons for gene expansion ..
  8. Wang K, Wloka C, Bi E. Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission. iScience. 2019;13:69-81 pubmed publisher
    ..The ESCRT-III component CHMP4B localizes to the midbody and "spreads" to the site of abscission only during late midbody stage...
  9. Webster B, Colombi P, Jäger J, Lusk C. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell. 2014;159:388-401 pubmed publisher
    ..Heh2 recruits the endosomal sorting complex required for transport (ESCRT)-III subunit Snf7 and the AAA-ATPase Vps4 to destabilize and clear defective NPC assembly intermediates...
  10. Kogan V, Millstein J, London S, Ober C, White S, Naureckas E, et al. Genetic-Epigenetic Interactions in Asthma Revealed by a Genome-Wide Gene-Centric Search. Hum Hered. 2018;83:130-152 pubmed publisher
    ..Twelve genes, PF4, ATF3, TPRA1, HOPX, SCARNA18, STC1, OR10K1, UPK1B, LOC101928523, LHX6, CHMP4B, and LANCL1, exhibited statistically significant SNP-CpG interactions (false discovery rate = 0.05)...
  11. Bleck M, Itano M, Johnson D, Thomas V, North A, Bieniasz P, et al. Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding. Proc Natl Acad Sci U S A. 2014;111:12211-6 pubmed publisher
    ..protein Tsg101 is recruited together with Gag to the sites of HIV-1 assembly, whereas later-acting ESCRT proteins (Chmp4b and Vps4A) are recruited sequentially, once Gag assembly is completed...
  12. Urquhart W, Mueller G, Carleton S, Song Z, Perez T, Uffman J, et al. A novel method of demonstrating the molecular and functional equivalence between in vitro and plant-produced double-stranded RNA. Regul Toxicol Pharmacol. 2015;73:607-12 pubmed publisher
    ..results in the formation of a double-stranded RNA (dsRNA) transcript containing a 240 bp fragment of the WCR Snf7 gene (DvSnf7) that confers resistance to corn rootworm by suppressing levels of DvSnf7 mRNA in WCR after root ..
  13. Schöneberg J, Pavlin M, Yan S, Righini M, Lee I, Carlson L, et al. ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science. 2018;362:1423-1428 pubmed publisher
    ..We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane ..
  14. Shen J, Liu Y, Song Y, Li L, Duan C, Zhou Y, et al. CHMP4B, ESCRT-III associating protein, associated with neuronal apoptosis following intracerebral hemorrhage. Brain Res. 2015;1597:1-13 pubmed publisher
    ..b>CHMP4B, a core component of the endosomal sorting complex required for transport (ESCRT)-III, is requisite for endosomal ..
  15. Kamranvar S, Gupta D, Huang Y, Gupta R, Johansson S. Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody. Oncotarget. 2016;7:30820-30 pubmed publisher
    ..fibroblasts, but it was unable to efficiently recruit ALIX, TSG101, and consequently the ESCRT-III subunit CHMP4B was missing in the non-adherent cells...
  16. Lee I, Kai H, Carlson L, Groves J, Hurley J. Negative membrane curvature catalyzes nucleation of endosomal sorting complex required for transport (ESCRT)-III assembly. Proc Natl Acad Sci U S A. 2015;112:15892-7 pubmed publisher
    ..The assembly of the core ESCRT-III subunit CHMP4B/Snf7 is preferentially nucleated in the resulting 100-nm-deep membrane concavities...
  17. Lone M, Kungl T, Koper A, Bottenberg W, KAMMERER R, Klein M, et al. The nuclear protein Waharan is required for endosomal-lysosomal trafficking in Drosophila. J Cell Sci. 2010;123:2369-74 pubmed publisher
    ..data demonstrating that Wah acts earlier in the trafficking pathway than the Escrt-III component Drosophila Shrb (snf7 in Schizosaccharomyces pombe), our results indicate that Wah is essential for endocytic trafficking at the late ..
  18. Cheng J, Yin Z, Zhang Z, Liang Y. Functional analysis of MoSnf7 in Magnaporthe oryzae. Fungal Genet Biol. 2018;121:29-45 pubmed publisher
    b>Snf7 is the core subunit protein of the yeast endosomal sorting complex required for transport (ESCRT) complex, which plays important roles in endocytosis and autophagy...
  19. Shim S, Merrill S, Hanson P. Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly. Mol Biol Cell. 2008;19:2661-72 pubmed publisher
    ..that it binds well to additional ESCRT-III proteins including CHMP1B, CHMP2A/hVps2-1, and CHMP3/hVps24 but not CHMP4A/hSnf7-1 or CHMP6/hVps20. LIP5 binds to a different region within CHMP5 than within the other ESCRT-III proteins...
  20. Cardona López X, Cuyas L, Marín E, Rajulu C, Irigoyen M, Gil E, et al. ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis. Plant Cell. 2015;27:2560-81 pubmed publisher
    ..Here, we describe ALIX, a cytosolic protein that associates with MVB by interacting with ESCRT-III subunit SNF7 and mediates PHT1;1 trafficking to the vacuole in Arabidopsis thaliana...
  21. Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P, et al. The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release. Structure. 2011;19:1485-95 pubmed publisher
    Alix and cellular paralogs HD-PTP and Brox contain N-terminal Bro1 domains that bind ESCRT-III CHMP4. In contrast to HD-PTP and Brox, expression of the Bro1 domain of Alix alleviates HIV-1 release defects that result from interrupted ..
  22. Zhang Y, Li W, Chu M, Chen H, Yu H, Fang C, et al. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ. Mycopathologia. 2016;181:329-39 pubmed publisher
    ..shown that at neutral-to-alkaline pH, hyphal growth is dependent on the Rim101 pathway whose activation requires Snf7, a member of the ESCRT system. In this work, we described the purification and characterization of the C...
  23. Shestakova A, Hanono A, Drosner S, Curtiss M, Davies B, Katzmann D, et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell. 2010;21:1059-71 pubmed publisher
    ..The most significant among these were the binding of Vps4 to Vta1 and to the ESCRT-III subunits Vps2 and Snf7. In our model we propose the formation of a recruitment complex in the cytoplasm that is composed of Did2-Ist1-Vps4,..
  24. Loncle N, Agromayor M, Martin Serrano J, Williams D. An ESCRT module is required for neuron pruning. Sci Rep. 2015;5:8461 pubmed publisher
    ..Furthermore we show that this ESCRT module requires a direct, in vivo, interaction between Shrub/CHMP4B and the accessory protein Myopic/HD-PTP.
  25. Petrick J, Frierdich G, Carleton S, Kessenich C, Silvanovich A, Zhang Y, et al. Corn rootworm-active RNA DvSnf7: Repeat dose oral toxicology assessment in support of human and mammalian safety. Regul Toxicol Pharmacol. 2016;81:57-68 pubmed publisher
    ..base pair (bp) dsRNA fragment targeting for suppression the western corn rootworm (Diabrotica virgifera virgifera) Snf7 gene (DvSnf7)...
  26. Prescher J, Baumgärtel V, Ivanchenko S, Torrano A, Bräuchle C, Muller B, et al. Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites. PLoS Pathog. 2015;11:e1004677 pubmed publisher
    ..super-resolution fluorescence microscopy to elucidate the size and structure of the ESCRT components Tsg101, ALIX, CHMP4B and CHMP2A during HIV-1 budding below the diffraction limit...
  27. Hilscher J, Kapusi E, Stoger E, Ibl V. Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm. Protoplasma. 2016;253:137-53 pubmed publisher
    ..We used fluorescently tagged core ESCRT-III members HvSNF7a/CHMP4 and HvVPS24/CHMP3 and the associated ESCRT-III component HvVPS60a/CHMP5 for transient localization studies in ..
  28. Robijns J, Molenberghs F, Sieprath T, Corne T, Verschuuren M, De Vos W. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. 2016;6:30325 pubmed publisher
    ..by the severity of the rupture event, and could be prolonged by knockdown of the ESCRT-III complex component CHMP4B. In conclusion, our approach reveals regulators of nuclear rupture induction and repair, which may have critical ..
  29. Terrell A, Anand D, Smith S, Dang C, Waters S, Pathania M, et al. Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes. Exp Eye Res. 2015;131:42-55 pubmed publisher
    ..Additionally, 21EM15 microarrays indicate expression of Chmp4b, Cryab and Tcfap2a among others important genes...
  30. Ost K, O Meara T, Huda N, Esher S, Alspaugh J. The Cryptococcus neoformans alkaline response pathway: identification of a novel rim pathway activator. PLoS Genet. 2015;11:e1005159 pubmed publisher
    ..Many of these genes are in fact required for Rim101 activation, including members of the ESCRT complex (Vps23 and Snf7), ESCRT-interacting proteins (Rim20 and Rim23), and the predicted Rim13 protease...
  31. Shen Q, Schuh A, Zheng Y, Quinney K, Wang L, Hanna M, et al. Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly. J Cell Biol. 2014;206:763-77 pubmed publisher
    ..Using cryogenic electron microscopy (cryo-EM), we found that the full-length ESCRT-III subunit Vps32/CHMP4B spontaneously forms single-stranded spiral filaments...
  32. Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
    ..In Saccharomyces cerevisiae, ESCRT-III consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their ..
  33. Hu B, Jiang D, Chen Y, Wei L, Zhang S, Zhao F, et al. High CHMP4B expression is associated with accelerated cell proliferation and resistance to doxorubicin in hepatocellular carcinoma. Tumour Biol. 2015;36:2569-81 pubmed publisher
    Charged multivesicular body protein 4B (CHMP4B), a subunit of the endosomal sorting complex required for transport (ESCRT)-III complex, plays an important part in cytokinetic membrane abscission and the late stage of mitotic cell division...
  34. Mu R, Dussupt V, Jiang J, Sette P, Rudd V, Chuenchor W, et al. Two distinct binding modes define the interaction of Brox with the C-terminal tails of CHMP5 and CHMP4B. Structure. 2012;20:887-98 pubmed publisher
    ..Here we demonstrate that hydrophobic residues at the CHMP4B C-terminal amphipathic α helix bind a concave surface of Brox, a mammalian paralog of Alix...
  35. Van Engelenburg S, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan S, et al. Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits. Science. 2014;343:653-6 pubmed publisher
    ..of budding virions and released particles, with head-localized levels of CHMP2A decreasing relative to Tsg101 and CHMP4B upon virus abscission...
  36. Zhao Y, Du J, Xiong B, Xu H, Jiang L. ESCRT components regulate the expression of the ER/Golgi calcium pump gene PMR1 through the Rim101/Nrg1 pathway in budding yeast. J Mol Cell Biol. 2013;5:336-44 pubmed publisher
    ..Here we show that deletion mutation of ESCRT components Snf7, Snf8, Stp22, Vps20, Vps25, Vps28, or Vps36 activates the calcium/calcineurin signaling in yeast cells, but ..
  37. Christiaens O, Prentice K, Pertry I, Ghislain M, Bailey A, Niblett C, et al. RNA interference: a promising biopesticide strategy against the African Sweetpotato Weevil Cylas brunneus. Sci Rep. 2016;6:38836 pubmed publisher
    ..The three best performing dsRNAs, targeting pros?2, rps13 and the homolog of Diabrotica virgifera snf7, were then used in further feeding trials to investigate RNAi by oral delivery...
  38. Sun S, Zhou X, Zhang W, Gallick G, Kuang J. Unravelling the pivotal role of Alix in MVB sorting and silencing of the activated EGFR. Biochem J. 2015;466:475-87 pubmed publisher
    ..via the Alix V domain, and increases Alix's association with membrane-bound charged multivesicular body protein 4 (CHMP4) via the Alix Bro1 domain...
  39. McMillan B, Tibbe C, Drabek A, Seegar T, Blacklow S, Klein T. Structural Basis for Regulation of ESCRT-III Complexes by Lgd. Cell Rep. 2017;19:1750-1757 pubmed publisher
    ..complex induces outward membrane budding and fission through homotypic polymerization of its core component Shrub/CHMP4B. Shrub activity is regulated by its direct interaction with a protein called Lgd in flies, or CC2D1A or B in ..
  40. Peñalva M, Lucena Agell D, Arst H. Liaison alcaline: Pals entice non-endosomal ESCRTs to the plasma membrane for pH signaling. Curr Opin Microbiol. 2014;22:49-59 pubmed publisher
    ..Pal proteins are insufficient to transmit the signal, which additionally requires ESCRT-I, II and Vps20 with Snf7 in ESCRT-III...
  41. Livramento K, Freitas N, Máximo W, Zanetti R, Paiva L. Gene Expression Profile Analysis is Directly Affected by the Selected Reference Gene: The Case of Leaf-Cutting Atta Sexdens. Insects. 2018;9: pubmed publisher
    ..The SNF7 gene expression pattern was stable in all evaluated sample set...
  42. Christ L, Wenzel E, Liestøl K, Raiborg C, Campsteijn C, Stenmark H. ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission. J Cell Biol. 2016;212:499-513 pubmed publisher
    ..The ESCRT-III subunit CHMP4B is a key effector in abscission, whereas its paralogue, CHMP4C, is a component in the abscission checkpoint that ..
  43. Yang H, Liu J, Lin J, Deng L, Fan S, Guo Y, et al. Overexpression of CHMP7 from rapeseed and Arabidopsis causes dwarfism and premature senescence in Arabidopsis. J Plant Physiol. 2016;204:16-26 pubmed publisher
    ..Compared with human CHMP7, we found that BnCHMP7 not only interacts with ESCRT-III subunits SNF7.2 (CHMP4B), but also with VPS2.2 and CHMP1B...
  44. Olmos Y, Hodgson L, Mantell J, Verkade P, Carlton J. ESCRT-III controls nuclear envelope reformation. Nature. 2015;522:236-9 pubmed publisher
    ..component charged multivesicular body protein 2A (CHMP2A) is directed to the forming NE through binding to CHMP4B, and provides an activity essential for NE reformation...
  45. Webster B, Thaller D, Jager J, Ochmann S, Borah S, Lusk C. Chm7 and Heh1 collaborate to link nuclear pore complex quality control with nuclear envelope sealing. EMBO J. 2016;35:2447-2467 pubmed
    ..We establish direct binding between Heh2 and the "open" forms of both Chm7 and the ESCRT-III, Snf7, and between Chm7 and Snf7...
  46. Mamińska A, Bartosik A, Banach Orłowska M, Pilecka I, Jastrzębski K, Zdżalik Bielecka D, et al. ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors. Sci Signal. 2016;9:ra8 pubmed publisher
    ..which mediate receptor trafficking and degradation, and found that the components Tsg101, Vps28, UBAP1, and CHMP4B were essential to restrict constitutive NF-κB signaling in human embryonic kidney 293 cells...
  47. Pocognoni C, Berberián M, Mayorga L. ESCRT (Endosomal Sorting Complex Required for Transport) Machinery Is Essential for Acrosomal Exocytosis in Human Sperm. Biol Reprod. 2015;93:124 pubmed publisher
    ..We report here that members of ESCRT I (TSG101), ESCRT III (CHMP4), and the AAA ATPase VPS4 are present in the acrosomal region of the human sperm...
  48. Zhai Q, Landesman M, Robinson H, Sundquist W, Hill C. Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding. PLoS ONE. 2011;6:e27466 pubmed publisher
    ..Human Bro1 domains share the ability to bind the CHMP4 subset of ESCRT-III proteins, associate with the HIV-1 NC(Gag) protein, and stimulate the budding of viral Gag ..
  49. Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, et al. The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling. PLoS Genet. 2015;11:e1005749 pubmed publisher
    ..We could show that CC2D1A and its interaction partner CHMP4B are localised on endosomes in MEF cells, when the activity of the endosomal protein VPS4 is reduced...
  50. Kovalev N, de Castro Martín I, Pogany J, Barajas D, Pathak K, Risco C, et al. Role of Viral RNA and Co-opted Cellular ESCRT-I and ESCRT-III Factors in Formation of Tombusvirus Spherules Harboring the Tombusvirus Replicase. J Virol. 2016;90:3611-26 pubmed publisher
    ..We also demonstrated the critical role of Snf7p (CHMP4), Vps20p, and Vps24p ESCRT-III proteins in tombusvirus replication in yeast and in vitro...
  51. Lee J, Oh K, Lee D, Kim B, Choi J, Ku B, et al. Structural Study of the HD-PTP Bro1 Domain in a Complex with the Core Region of STAM2, a Subunit of ESCRT-0. PLoS ONE. 2016;11:e0149113 pubmed publisher
    ..and is associated with EGFR trafficking by interacting with the ESCRT-0 protein STAM2 and the ESCRT-III protein CHMP4B via its N-terminal Bro1 domain...
  52. Chiaruttini N, Redondo Morata L, Colom A, Humbert F, Lenz M, Scheuring S, et al. Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation. Cell. 2015;163:866-79 pubmed publisher
    ..However, how ESCRT-III polymerization generates membrane curvature remains debated. Here, we show that Snf7, the main component of ESCRT-III, polymerizes into spirals at the surface of lipid bilayers...
  53. Mogilicherla K, Howell J, Palli S. Improving RNAi in the Brown Marmorated Stink Bug: Identification of target genes and reference genes for RT-qPCR. Sci Rep. 2018;8:3720 pubmed publisher
    ..Feeding dsRNA targeting five of these genes (IAP, ATPase, SNF7, GPCR, and PPI) to nymphs caused more than 70% mortality by three of the five dsRNAs tested...
  54. Gahloth D, Levy C, Walker L, Wunderley L, Mould A, Taylor S, et al. Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP. Structure. 2017;25:1011-1024.e4 pubmed publisher
    ..between the SARA/endofin N termini, and the conserved hydrophobic region in the HD-PTP Bro1 domain that binds CHMP4/ESCRT-III...
  55. Rodrigues J, Lydall D. Cis and trans interactions between genes encoding PAF1 complex and ESCRT machinery components in yeast. Curr Genet. 2018;64:1105-1116 pubmed publisher
    ..lethal with deletions of other components of the ESCRT-II (SNF8 and VPS25), ESCRT-I (STP22), or ESCRT-III (SNF7) complexes...
  56. Dema A, Macaluso F, Sgrò F, Berto G, Bianchi F, Chiotto A, et al. Citron kinase-dependent F-actin maintenance at midbody secondary ingression sites mediates abscission. J Cell Sci. 2018;131: pubmed publisher
    ..midbody severing is mediated by formation of secondary midbody ingression sites, where the ESCRT III component CHMP4B is recruited to mediate membrane fusion...
  57. Dores M, Lin H, J Grimsey N, Méndez F, Trejo J. The α-arrestin ARRDC3 mediates ALIX ubiquitination and G protein-coupled receptor lysosomal sorting. Mol Biol Cell. 2015;26:4660-73 pubmed publisher
    ..Depletion of ARRDC3 by small interfering RNA disrupts ALIX interaction with activated PAR1 and the CHMP4B ESCRT-III subunit, suggesting that ARRDC3 regulates ALIX activity...
  58. Buono R, Leier A, Paez Valencia J, Pennington J, Goodman K, Miller N, et al. ESCRT-mediated vesicle concatenation in plant endosomes. J Cell Biol. 2017;216:2167-2177 pubmed publisher
    ..Consistent with a potential role as a diffusion barrier, we find that the ESCRT-III protein SNF7 remains associated with ILVs and is delivered to the vacuole for degradation.
  59. Tang S, Buchkovich N, Henne W, Banjade S, Kim Y, Emr S. ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis. elife. 2016;5: pubmed publisher
    ..Previously, we determined X-ray crystal structures of ESCRT-III subunit Snf7, the yeast CHMP4 ortholog, in its active and polymeric state (Tang et al., 2015)...
  60. Bulgari D, Jha A, Deitcher D, Levitan E. Myopic (HD-PTP, PTPN23) selectively regulates synaptic neuropeptide release. Proc Natl Acad Sci U S A. 2018;115:1617-1622 pubmed publisher
    ..endosomes, but interaction with the endosomal sorting complex required for transport III (ESCRT III) protein (CHMP4/Shrub) that mediates Myopic's effect on neuron pruning is not required for control of neuropeptide release...
  61. Wolters N, Amerik A. The N-terminal domains determine cellular localization and functions of the Doa4 and Ubp5 deubiquitinating enzymes. Biochem Biophys Res Commun. 2015;467:570-6 pubmed publisher
    ..Here we show that Doa4 physically interacts with the ESCRT-III component Snf7 and preferentially cleaves Lys63-linked ubiquitin oligomers involved in membrane protein trafficking...
  62. Bartusch C, Prange R. ESCRT Requirements for Murine Leukemia Virus Release. Viruses. 2016;8:103 pubmed publisher
    ..the effects of individual ESCRT-III knockdowns, VLP and virion release was profoundly inhibited in CHMP2A- and CHMP4B-knockdown cells...
  63. Tang S, Henne W, Borbat P, Buchkovich N, Freed J, Mao Y, et al. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. elife. 2015;4: pubmed publisher
    ..Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation...
  64. Deshar R, Cho E, Yoon S, Yoon J. CC2D1A and CC2D1B regulate degradation and signaling of EGFR and TLR4. Biochem Biophys Res Commun. 2016;480:280-287 pubmed publisher
    ..Here, we report that CC2D1A binds to CHMP4B polymers formed on endosomes to regulate the endosomal sorting pathway...
  65. Adell M, Migliano S, Upadhyayula S, Bykov Y, Sprenger S, Pakdel M, et al. Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding. elife. 2017;6: pubmed publisher
    ..During their 3-45 s lifetimes, the ESCRT-III assemblies accumulated 75-200 Snf7 and 15-50 Vps24 molecules. Productive budding events required at least two additional Vps4 hexamers...
  66. Ali N, Zhang L, Taylor S, Mironov A, Urbé S, Woodman P. Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB. Curr Biol. 2013;23:453-61 pubmed publisher
    ..First, the HD-PTP Bro1 domain binds the core domain of STAM2. This is competed by the ESCRT-III subunit CHMP4B, which binds an overlapping site on HD-PTP Bro1...
  67. Boonyaratanakornkit J, Schomacker H, Collins P, Schmidt A. Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system. PLoS ONE. 2013;8:e59462 pubmed publisher
    ..interact with the Bro1 domain of Alix at a site that is also required for the interaction between Alix and Chmp4b, a subunit of ESCRT-III...
  68. Tabata K, Arimoto M, Arakawa M, Nara A, Saito K, Omori H, et al. Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum. Cell Rep. 2016;16:2339-47 pubmed publisher
    ..Electron-microscopy studies revealed that the CHMP2 and CHMP4 proteins function directly in membrane deformation at the ER...
  69. Usami Y, Popov S, Weiss E, Vriesema Magnuson C, Calistri A, Gottlinger H. Regulation of CHMP4/ESCRT-III function in human immunodeficiency virus type 1 budding by CC2D1A. J Virol. 2012;86:3746-56 pubmed publisher
    ..Both proteins bind to the core domain of CHMP4B, which has a strong propensity to polymerize and to inhibit HIV-1 budding...
  70. Xu B, Fu Y, Liu Y, Agvanian S, Wirka R, Baum R, et al. The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles. PLoS Biol. 2017;15:e2002354 pubmed publisher
    ..endosomal sorting complexes required for transport (ESCRT)-III subunit charged multivesicular body protein 4B (CHMP4B) colocalizes and coimmunoprecipitates with cBIN1, an interaction enhanced by actin stabilization...
  71. Kadiu I, Gendelman H. Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection. J Neuroimmune Pharmacol. 2011;6:658-75 pubmed publisher
    ..These data lead us to propose that HIV-1 hijacks macrophage endocytic and cytoskeletal machineries for high-speed cell-to-cell spread. ..
  72. Row P, Liu H, Hayes S, Welchman R, Charalabous P, Hofmann K, et al. The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degradation. J Biol Chem. 2007;282:30929-37 pubmed
  73. Ding X, Stewart M, Sharei A, Weaver J, Langer R, Jensen K. High-throughput Nuclear Delivery and Rapid Expression of DNA via Mechanical and Electrical Cell-Membrane Disruption. Nat Biomed Eng. 2017;1: pubmed publisher
    ..By tracking the localization of the ESCRT-III (endosomal sorting complexes required for transport) protein CHMP4B, we show that the integrity of the nuclear envelope is recovered within 15 minutes of treatment...
  74. Shi T, Dong Y, Li J, Gao P, Fu D, Ma D. High-throughput screening identifies CHMP4A associated with hypoxia-inducible factor 1. Life Sci. 2010;87:604-8 pubmed publisher
  75. Lee J, Liu L, Javier R, Kreitzer A, Delaloy C, Gao F. ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate transmembrane cargos in hESC-derived human neurons. Mol Brain. 2011;4:37 pubmed publisher
    ..Moreover, we found that among the three human paralogs of the yeast ESCRT-III subunit Snf7, hSnf7-1 and hSnf7-2 are most abundantly expressed in human neurons...
  76. Dores M, Chen B, Lin H, Soh U, Paing M, Montagne W, et al. ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting. J Cell Biol. 2012;197:407-19 pubmed publisher
    ..This study reveals a novel MVB/lysosomal sorting pathway for signaling receptors that bypasses the requirement for ubiquitination and ubiquitin-binding ESCRTs and may be applicable to a subset of GPCRs containing YPX(n)L motifs. ..
  77. Capalbo L, Montembault E, Takeda T, Bassi Z, Glover D, D Avino P. The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis. Open Biol. 2012;2:120070 pubmed publisher
    ..Here, we show that the CPC subunit Borealin interacts directly with the Snf7 components of ESCRT-III in both Drosophila and human cells...
  78. Curwin A, Brouwers N, Alonso Y Adell M, Teis D, Turacchio G, Parashuraman S, et al. ESCRT-III drives the final stages of CUPS maturation for unconventional protein secretion. elife. 2016;5: pubmed publisher
    ..The major ESCRT-III subunit Snf7 localizes transiently to CUPS and this was accelerated in vps4? cells, correlating with increased Acb1 secretion...
  79. Cashikar A, Shim S, Roth R, Maldazys M, Heuser J, Hanson P. Structure of cellular ESCRT-III spirals and their relationship to HIV budding. elife. 2014;3: pubmed publisher
    ..Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral...
  80. McMillan B, Tibbe C, Jeon H, Drabek A, Klein T, Blacklow S. Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein. Cell Rep. 2016;16:1211-1217 pubmed publisher
    ..The ESCRT-III protein Shrub in flies, or its homologs in yeast (Snf7) or humans (CHMP4B), is a critical polymerizing component of ESCRT-III needed to effect membrane fission...
  81. Jouvenet N, Simon S, Bieniasz P. Visualizing HIV-1 assembly. J Mol Biol. 2011;410:501-11 pubmed publisher
    ..Overall, the particular advantages of individual particle imaging in living cells have yielded findings that would have been difficult or impossible to obtain using macroscopic or fixed-cell microscopic techniques. ..
  82. Carlson L, Shen Q, Pavlin M, Hurley J. ESCRT Filaments as Spiral Springs. Dev Cell. 2015;35:397-8 pubmed publisher
    ..2015) reveal the mechanical properties of the mysterious spiral filaments formed by the yeast ESCRT-III protein Snf7. The spirals are shown to be springs whose bending drives membrane deformation and perhaps membrane scission.
  83. Mierzwa B, Chiaruttini N, Redondo Morata L, von Filseck J, König J, Larios J, et al. Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nat Cell Biol. 2017;19:787-798 pubmed publisher
    ..In vitro, the Vps2/Vps24 subunits of ESCRT-III formed side-by-side filaments with Snf7 and inhibited further polymerization, but the growth inhibition was alleviated by the addition of Vps4 and ATP...
  84. Vita D, Broadie K. ESCRT-III Membrane Trafficking Misregulation Contributes To Fragile X Syndrome Synaptic Defects. Sci Rep. 2017;7:8683 pubmed publisher
    ..In the Drosophila FXS disease model, we found FMRP binds shrub mRNA (human Chmp4) to repress Shrub expression, causing overexpression during the disease state early-use critical period...
  85. Yue Q, Yu Q, Yang Q, Xu Y, Guo Y, Blissard G, et al. Distinct Roles of Cellular ESCRT-I and ESCRT-III Proteins in Efficient Entry and Egress of Budded Virions of Autographa californica Multiple Nucleopolyhedrovirus. J Virol. 2018;92: pubmed publisher
    ..In this study, the core components of ESCRT-I (Tsg101 and Vps28) and ESCRT-III (Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral complementation system and RNA ..
  86. Baumgärtel V, Muller B, Lamb D. Quantitative live-cell imaging of human immunodeficiency virus (HIV-1) assembly. Viruses. 2012;4:777-99 pubmed publisher
  87. Sandrin V, Sundquist W. ESCRT requirements for EIAV budding. Retrovirology. 2013;10:104 pubmed publisher
    ..wild type and mutant constructs revealed budding requirements for the following ESCRT proteins: ALIX, CHMP4B, CHMP2A and VPS4A or VPS4B...
  88. Rauch S, Martin Serrano J. Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding. J Virol. 2011;85:3546-56 pubmed publisher
    ..Taken together, our results support a model whereby ARTs are involved in PPXY-mediated budding by interacting with HECT ubiquitin ligases and providing several alternative routes for ESCRT-III recruitment. ..
  89. Pereira A, Velez A, Meinke L, Siegfried B. Sublethal Effects of vATPase-A and Snf7 dsRNAs on Biology of Southern Corn Rootworm, Diabrotica undecimpunctata howardi Barber. J Econ Entomol. 2017;: pubmed publisher
    ..Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae), were exposed to the LC50 of vATPase-A and Snf7 double-stranded RNAs (dsRNAs), and the effects on female fecundity, egg viability, male fitness as measured by ..