CHMP3

Summary

Gene Symbol: CHMP3
Description: charged multivesicular body protein 3
Alias: CGI-149, NEDF, VPS24, charged multivesicular body protein 3, 25.1 protein, CHMP family, member 3, chromatin-modifying protein 3, neuroendocrine differentiation factor, vacuolar protein sorting 24 homolog, vacuolar protein sorting-associated protein 24
Species: human
Products:     CHMP3

Top Publications

  1. Zamborlini A, Usami Y, Radoshitzky S, Popova E, Palu G, Gottlinger H. Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Proc Natl Acad Sci U S A. 2006;103:19140-5 pubmed
    ..Together with biochemical evidence for specific intramolecular interactions between the basic and acidic halves of CHMP3 and CHMP4B, these results suggest that the acidic domains are autoinhibitory...
  2. Merrill S, Hanson P. Activation of human VPS4A by ESCRT-III proteins reveals ability of substrates to relieve enzyme autoinhibition. J Biol Chem. 2010;285:35428-38 pubmed publisher
    ..Importantly, C-terminal fragments of all ESCRT-III proteins tested, including CHMP2A, CHMP1B, CHMP3, CHMP4A, CHMP6, and CHMP5, activated VPS4A suggesting that it disassembles ESCRT-III heteropolymers by affecting ..
  3. Kuang Z, Seo E, Leis J. Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15. J Virol. 2011;85:7153-61 pubmed publisher
    ..CHMP5 is the primary switch to initiate the antiviral mechanism, because removal of CHMP5 from cells prevents ISGylation of CHMP2A and CHMP6. ..
  4. Effantin G, Dordor A, Sandrin V, Martinelli N, Sundquist W, Schoehn G, et al. ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding. Cell Microbiol. 2013;15:213-26 pubmed publisher
    ..We have used electron cryomicroscopy to determine the molecular organization of pleiomorphic ESCRT-III CHMP2A-CHMP3 polymers...
  5. von Schwedler U, Stuchell M, Muller B, Ward D, Chung H, Morita E, et al. The protein network of HIV budding. Cell. 2003;114:701-13 pubmed
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses. ..
  6. Lin Y, Kimpler L, Naismith T, Lauer J, Hanson P. Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1. J Biol Chem. 2005;280:12799-809 pubmed
    ..Together with earlier studies, our work suggests that a variety of ESCRT-III-containing polymers can assemble on membranes and recruit SKD1 during formation of the MVB. ..
  7. Shim S, Merrill S, Hanson P. Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly. Mol Biol Cell. 2008;19:2661-72 pubmed publisher
    ..to CHMP5, but also find that it binds well to additional ESCRT-III proteins including CHMP1B, CHMP2A/hVps2-1, and CHMP3/hVps24 but not CHMP4A/hSnf7-1 or CHMP6/hVps20...
  8. Lata S, Schoehn G, Jain A, Pires R, Piehler J, Gottlinger H, et al. Helical structures of ESCRT-III are disassembled by VPS4. Science. 2008;321:1354-7 pubmed publisher
    ..We found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures that ..
  9. Muzioł T, Pineda Molina E, Ravelli R, Zamborlini A, Usami Y, Gottlinger H, et al. Structural basis for budding by the ESCRT-III factor CHMP3. Dev Cell. 2006;10:821-30 pubmed
    ..Here we report the crystal structure of the human ESCRT-III protein CHMP3 at 2.8 A resolution...

More Information

Publications53

  1. Strack B, Calistri A, Craig S, Popova E, Gottlinger H. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689-99 pubmed
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  2. Row P, Liu H, Hayes S, Welchman R, Charalabous P, Hofmann K, et al. The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degradation. J Biol Chem. 2007;282:30929-37 pubmed
    ..isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7...
  3. Morita E, Colf L, Karren M, Sandrin V, Rodesch C, Sundquist W. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010;107:12889-94 pubmed publisher
  4. Babst M, Katzmann D, Estepa Sabal E, Meerloo T, Emr S. Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell. 2002;3:271-82 pubmed
    ..The soluble coiled-coil-containing proteins Vps2, Vps20, Vps24, and Snf7 are recruited from the cytoplasm to endosomal membranes where they oligomerize into a protein complex, ..
  5. Hurley J. Nipped in the bud: how the AMSH MIT domain helps deubiquitinate lysosome-bound cargo. Structure. 2011;19:1033-5 pubmed publisher
    ..In this issue of Structure, Solomons et al. now reveal an extraordinarily high affinity complex between the "MIM4" region of one ESCRT-III subunit, CHMP3, and the MIT domain of AMSH.
  6. Firkowska M, Macias M, Jaworski J. ESCRT Proteins Control the Dendritic Morphology of Developing and Mature Hippocampal Neurons. Mol Neurobiol. 2018;: pubmed publisher
    ..We also demonstrated that Vps28, Vps24, and Vps25 are required for dendrite stabilization in mature neurons.
  7. Schöneberg J, Pavlin M, Yan S, Righini M, Lee I, Carlson L, et al. ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science. 2018;362:1423-1428 pubmed publisher
    ..We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes ..
  8. Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
    ..In Saccharomyces cerevisiae, ESCRT-III consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their disassembly...
  9. Yang B, Stjepanovic G, Shen Q, Martin A, Hurley J. Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation. Nat Struct Mol Biol. 2015;22:492-8 pubmed publisher
    ..we carried out hydrogen/deuterium exchange during Saccharomyces cerevisiae Vps4 disassembly of a chimeric Vps24-2 ESCRT-III filament...
  10. Shtanko O, Nikitina R, Altuntas C, Chepurnov A, Davey R. Crimean-Congo hemorrhagic fever virus entry into host cells occurs through the multivesicular body and requires ESCRT regulators. PLoS Pathog. 2014;10:e1004390 pubmed publisher
    ..Silencing Tsg101, Vps24, Vps4B, or Alix/Aip1, components of the endosomal sorting complex required for transport (ESCRT) pathway ..
  11. Koumanov F, Pereira V, Whitley P, Holman G. GLUT4 traffic through an ESCRT-III-dependent sorting compartment in adipocytes. PLoS ONE. 2012;7:e44141 pubmed publisher
    ..Introduction of the dominant negative inhibitory constructs of the ESCRT-III components CHMP3 (CHMP3(1-179)) and Vps4 (GFP-Vps4(E235Q)) into rat adipocytes leads to the accumulation of GLUT4 in large, ..
  12. Hilscher J, Kapusi E, Stoger E, Ibl V. Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm. Protoplasma. 2016;253:137-53 pubmed publisher
    ..We used fluorescently tagged core ESCRT-III members HvSNF7a/CHMP4 and HvVPS24/CHMP3 and the associated ESCRT-III component HvVPS60a/CHMP5 for transient localization studies in barley endosperm...
  13. Li C, Bazzano L, Rao D, Hixson J, He J, Gu D, et al. Genome-wide linkage and positional association analyses identify associations of novel AFF3 and NTM genes with triglycerides: the GenSalt study. J Genet Genomics. 2015;42:107-17 pubmed publisher
    ..Follow-up analyses of these two regions revealed gene-based associations of charged multivesicular body protein 3 (CHMP3), ring finger protein 103 (RNF103), AF4/FMR2 family, member 3 (AFF3), and neurotrimin (NTM) ..
  14. Ishii A, Kawai M, Noda H, Kato H, Takeda K, Asakawa K, et al. Accelerated invagination of vacuoles as a stress response in chronically heat-stressed yeasts. Sci Rep. 2018;8:2644 pubmed publisher
    ..occurred in the absence of Atg1, Atg9 or Ivy1 but appeared to require endosomal sorting proteins, such as Vps23, Vps24 or Pep12...
  15. Banh B, McDERMOTT H, Woodman S, Gadila S, Saimani U, Short J, et al. Yeast dynamin interaction with ESCRT proteins at the endosome. Cell Biol Int. 2017;41:484-494 pubmed publisher
    ..we revealed that Vps1 physically interacts with the ESCRT-II subunits, Vps22 and Vps36, and the ESCRT-III subunit Vps24. We found that Vps1 and ESCRT-II components colocalize with Pep12, an endosomal marker...
  16. Jiang B, Himmelsbach K, Ren H, Boller K, Hildt E. Subviral Hepatitis B Virus Filaments, like Infectious Viral Particles, Are Released via Multivesicular Bodies. J Virol. 2015;90:3330-41 pubmed publisher
    ..U18666A or inhibition of ESCRT functionality by coexpression of transdominant negative mutants (Vps4A, Vps4B, and CHMP3) abolishes the release of filaments while the secretion of spheres is not affected...
  17. Bartusch C, Prange R. ESCRT Requirements for Murine Leukemia Virus Release. Viruses. 2016;8:103 pubmed publisher
    ..In contrast, neither the CHMP2B and CHMP4A isoforms nor CHMP3, CHMP5, and CHMP6 were found to be essential...
  18. Walker G, Antoniono R, Ross H, Paisley T, Oh Y. Neuroendocrine-like differentiation of non-small cell lung carcinoma cells: regulation by cAMP and the interaction of mac25/IGFBP-rP1 and 25.1. Oncogene. 2006;25:1943-54 pubmed
    ..1 may play a functional role in the NE differentiation of NSCLC cell lines and may provide a novel therapeutic target for treating lung cancers, in particular NSCLC with NE differentiation. ..
  19. Salvi M, Raiborg C, Hanson P, Campsteijn C, Stenmark H, Pinna L. CK2 involvement in ESCRT-III complex phosphorylation. Arch Biochem Biophys. 2014;545:83-91 pubmed publisher
    ..Here we show that protein kinase CK2? is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly...
  20. Kyuuma M, Kikuchi K, Kojima K, Sugawara Y, Sato M, Mano N, et al. AMSH, an ESCRT-III associated enzyme, deubiquitinates cargo on MVB/late endosomes. Cell Struct Funct. 2007;31:159-72 pubmed
    ..Here, we purified an AMSH-binding protein, CHMP3, which is an ESCRT-III subunit...
  21. Lata S, Roessle M, Solomons J, Jamin M, Gottlinger H, Svergun D, et al. Structural basis for autoinhibition of ESCRT-III CHMP3. J Mol Biol. 2008;378:818-27 pubmed publisher
    ..Here we provide structural evidence based on small-angle X-ray scattering data that ESCRT-III CHMP3 can adopt two conformations in solution: a closed globular form that most likely represents the cytosolic ..
  22. Skalicky J, Arii J, Wenzel D, Stubblefield W, Katsuyama A, Uter N, et al. Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport. J Biol Chem. 2012;287:43910-26 pubmed publisher
    ..Our studies thus reveal how the tandem MIT domain of LIP5 binds different types of ESCRT-III proteins, promoting assembly of active VPS4 enzymes on the polymeric ESCRT-III substrate. ..
  23. Agromayor M, Martin Serrano J. Interaction of AMSH with ESCRT-III and deubiquitination of endosomal cargo. J Biol Chem. 2006;281:23083-91 pubmed
    ..enzyme, with the endodomal sorting complex required for transport (ESCRT-III) subunits CHMP1A, CHMP1B, CHMP2A, and CHMP3. We also show that a catalytically inactive AMSH inhibits retroviral budding in a dominant-negative manner and ..
  24. Yan Q, Hunt P, Frelin L, Vida T, Pevsner J, Bean A. mVps24p functions in EGF receptor sorting/trafficking from the early endosome. Exp Cell Res. 2005;304:265-73 pubmed
    ..These findings are consistent with a model in which mVps24p has a role in trafficking from the early endosome. ..
  25. Carlson L, Hurley J. In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters. Proc Natl Acad Sci U S A. 2012;109:16928-33 pubmed publisher
    ..pathway of ESCRT recruitment to HIV-1 budding sites, which culminates with the assembly of the late-acting CHMP4, CHMP3, CHMP2, and CHMP1 subunits, is less completely understood...
  26. Khoury C, Yang Z, Ismail S, Greenwood M. Characterization of a novel alternatively spliced human transcript encoding an N-terminally truncated Vps24 protein that suppresses the effects of Bax in an ESCRT independent manner in yeast. Gene. 2007;391:233-41 pubmed
    ..6, 751-762]. Here we report that one of the Bax suppressors encodes a novel 156 amino acid variant of the human Vps24 protein, Vps24beta, that lacks the N-terminal lipid binding domain of the well characterized 222 residue Vps24 (..
  27. Baietti M, Zhang Z, Mortier E, Melchior A, Degeest G, Geeraerts A, et al. Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012;14:677-85 pubmed publisher
    ..This study identifies a key role for syndecan-syntenin-ALIX in membrane transport and signalling processes. ..
  28. Metcalf D, Isaacs A. The role of ESCRT proteins in fusion events involving lysosomes, endosomes and autophagosomes. Biochem Soc Trans. 2010;38:1469-73 pubmed publisher
  29. Bache K, Stuffers S, Malerød L, Slagsvold T, Raiborg C, Lechardeur D, et al. The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor. Mol Biol Cell. 2006;17:2513-23 pubmed
    ..the importance of the ESCRT-I subunit tumor susceptibility gene 101 (Tsg101) and the ESCRT-III subunit hVps24/CHMP3 for endosomal functions and receptor signaling. Like Tsg101, endogenous hVps24 localized mainly to late endosomes...
  30. Calistri A, Sette P, Salata C, Cancellotti E, Forghieri C, Comin A, et al. Intracellular trafficking and maturation of herpes simplex virus type 1 gB and virus egress require functional biogenesis of multivesicular bodies. J Virol. 2007;81:11468-78 pubmed
    ..By expression of dominant negative forms of Vps4 and Vps24, two components of the MVB pathway, we observed an impairment in infectious herpes simplex virus (HSV) assembly/..
  31. Su Z, Kang D, Chen Y, Pekarskaya O, Chao W, Volsky D, et al. Identification of gene products suppressed by human immunodeficiency virus type 1 infection or gp120 exposure of primary human astrocytes by rapid subtraction hybridization. J Neurovirol. 2003;9:372-89 pubmed
  32. Bajorek M, Schubert H, McCullough J, Langelier C, Eckert D, Stubblefield W, et al. Structural basis for ESCRT-III protein autoinhibition. Nat Struct Mol Biol. 2009;16:754-62 pubmed publisher
    ..that the N-terminal core domains of increased sodium tolerance-1 (IST1) and charged multivesicular body protein-3 (CHMP3) form equivalent four-helix bundles, revealing that IST1 is a previously unrecognized ESCRT-III family member...
  33. Solomons J, Sabin C, Poudevigne E, Usami Y, Hulsik D, Macheboeuf P, et al. Structural basis for ESCRT-III CHMP3 recruitment of AMSH. Structure. 2011;19:1149-59 pubmed publisher
    ..fragment of the deubiquitinating enzyme AMSH (AMSHΔC) in complex with the C-terminal region of ESCRT-III CHMP3 (CHMP3ΔN). AMSHΔC folds into an elongated 90 Å long helical assembly that includes an unusual MIT domain...
  34. Cashikar A, Shim S, Roth R, Maldazys M, Heuser J, Hanson P. Structure of cellular ESCRT-III spirals and their relationship to HIV budding. elife. 2014;3: pubmed publisher
    ..Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral...
  35. Popova E, Popov S, Gottlinger H. Human immunodeficiency virus type 1 nucleocapsid p1 confers ESCRT pathway dependence. J Virol. 2010;84:6590-7 pubmed publisher
    ..Together, these results indicate that the ESCRT pathway dependence of HIV-1 budding is determined, at least in part, by the NC-p1 region of Gag...
  36. McCullough J, Row P, Lorenzo O, Doherty M, Beynon R, Clague M, et al. Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery. Curr Biol. 2006;16:160-5 pubmed
    ..AMSH and STAM, like Hrs , both bind directly to clathrin. AMSH also interacts with mVps24/CHMP3, a component of ESCRT III complex, and this interaction is reinforced through simultaneous STAM binding...
  37. Mierzwa B, Chiaruttini N, Redondo Morata L, von Filseck J, König J, Larios J, et al. Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nat Cell Biol. 2017;19:787-798 pubmed publisher
    ..In vitro, the Vps2/Vps24 subunits of ESCRT-III formed side-by-side filaments with Snf7 and inhibited further polymerization, but the growth ..
  38. Whitley P, Reaves B, Hashimoto M, Riley A, Potter B, Holman G. Identification of mammalian Vps24p as an effector of phosphatidylinositol 3,5-bisphosphate-dependent endosome compartmentalization. J Biol Chem. 2003;278:38786-95 pubmed publisher
    ..We hypothesize that accumulation of Vps24 on membranes occurs when membrane association (dependent on interaction of phosphatidylinositol 3,5-bisphosphate ..
  39. Adell M, Migliano S, Upadhyayula S, Bykov Y, Sprenger S, Pakdel M, et al. Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding. elife. 2017;6: pubmed publisher
    ..During their 3-45 s lifetimes, the ESCRT-III assemblies accumulated 75-200 Snf7 and 15-50 Vps24 molecules. Productive budding events required at least two additional Vps4 hexamers...
  40. Wilson E, Oh Y, Hwa V, Rosenfeld R. Interaction of IGF-binding protein-related protein 1 with a novel protein, neuroendocrine differentiation factor, results in neuroendocrine differentiation of prostate cancer cells. J Clin Endocrinol Metab. 2001;86:4504-11 pubmed
    ..We propose that 25.1 (neuroendocrine differentiation factor) together with IGF-binding protein-related protein 1 can induce neuroendocrine cell ..
  41. Yue Q, Yu Q, Yang Q, Xu Y, Guo Y, Blissard G, et al. Distinct Roles of Cellular ESCRT-I and ESCRT-III Proteins in Efficient Entry and Egress of Budded Virions of Autographa californica Multiple Nucleopolyhedrovirus. J Virol. 2018;92: pubmed publisher
    ..In this study, the core components of ESCRT-I (Tsg101 and Vps28) and ESCRT-III (Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral complementation system and ..
  42. Ma Y, Boucrot E, Villen J, Affar E, Gygi S, Gottlinger H, et al. Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation. J Biol Chem. 2007;282:9805-12 pubmed
    ..Here we report that CHMP3, an ESCRT-III complex component, and associated molecule of SH3 domain of STAM (AMSH), a deubiquitinating enzyme, ..
  43. Martin Serrano J, Yarovoy A, Perez Caballero D, Bieniasz P, Yaravoy A. Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc Natl Acad Sci U S A. 2003;100:12414-9 pubmed
  44. Saha N, Dutta S, Datta S, Sarkar S. The minimal ESCRT machinery of Giardia lamblia has altered inter-subunit interactions within the ESCRT-II and ESCRT-III complexes. Eur J Cell Biol. 2018;97:44-62 pubmed publisher
    ..Interactions within the giardial ESCRT-III components also differ from those in yeast; while GlVps46a interacts preferentially with Vps24 compared to Vps2, GlVps46b, like the yeast orthologue, interacts with both.