CELA3B

Summary

Gene Symbol: CELA3B
Description: chymotrypsin-like elastase family, member 3B
Alias: CBPP, EL-1, ELA3B, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, elastase 1, elastase 3B, pancreatic, elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, protease E, proteinase E
Species: human

Top Publications

  1. ncbi The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase
    A B Cohen
    Biochim Biophys Acta 391:193-200. 1975
  2. doi Low fecal elastase 1 levels do not indicate exocrine pancreatic insufficiency in type-1 diabetes mellitus
    Jan Uwe Hahn
    Clinic for Diabetes and Metabolic Diseases, Klinikum Karlsburg, Karlsburg, Germany
    Pancreas 36:274-8. 2008
  3. ncbi Pancreatic exocrine dysfunction in maturity-onset diabetes of the young type 3
    Mette Vesterhus
    Department of Pediatrics, Haukeland University Hospital, Bergen, Norway
    Diabetes Care 31:306-10. 2008
  4. ncbi The elimination of meat from the diet selectively decreases pancreatic elastase secretion
    Jaroslaw Walkowiak
    Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, Poznan, Poland
    Br J Nutr 98:154-8. 2007
  5. ncbi Pancreatin therapy in patients with insulin-treated diabetes mellitus and exocrine pancreatic insufficiency according to low fecal elastase 1 concentrations. Results of a prospective multi-centre trial
    Nils Ewald
    Third Medical Department and Policlinic, Giessen University Hospital, Giessen, Germany
    Diabetes Metab Res Rev 23:386-91. 2007
  6. ncbi Evaluation of fecal pancreatic elastase-1 as a measure of pancreatic exocrine function in children with cystic fibrosis
    A Cade
    Regional Paediatric Cystic Fibrosis Unit, St James s University Hospital, Leeds, UK
    Pediatr Pulmonol 29:172-6. 2000
  7. ncbi Fecal elastase 1 and vitamin D3 in patients with osteoporotic bone fractures
    Sacha T W Mann
    Department of Internal Medicine, Medical Clinic III and Polyclinic of the Justus Liebig University, Giessen, Germany
    Eur J Med Res 13:68-72. 2008
  8. ncbi Pancreatic elastase-1 in stools, a marker of exocrine pancreas function, correlates with both residual beta-cell secretion and metabolic control in type 1 diabetic subjects
    Franco Cavalot
    University of Turin, San Luigi Gonzaga Hospital, Department of Clinical and Biological Sciences, Diabetes Unit, 10043 Orbassano TO, Turin, Italy
    Diabetes Care 27:2052-4. 2004
  9. ncbi Parathormone levels and Vitamin D metabolism in female patients with various grades of fecal elastase 1 deficiency
    J Teichmann
    Department of Internal Medicine, Medical Clinc C, Ludwigshafen, Germany
    Eur J Med Res 13:563-7. 2008
  10. ncbi Faecal pancreatic elastase--1 a non invasive measure of exocrine pancreatic function
    S D Amanquah
    Department of Chemical Pathology, University of Ghana Medical School, Accra, Ghana
    West Afr J Med 23:240-4. 2004

Scientific Experts

Detail Information

Publications126 found, 100 shown here

  1. ncbi The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase
    A B Cohen
    Biochim Biophys Acta 391:193-200. 1975
    ..The variable susceptibility of the enzyme to blockage of the inhibition by low concentrations of p-dioxane suggested that hydrophobic bonds may be important in the interaction with alpha-1-antitrypsin...
  2. doi Low fecal elastase 1 levels do not indicate exocrine pancreatic insufficiency in type-1 diabetes mellitus
    Jan Uwe Hahn
    Clinic for Diabetes and Metabolic Diseases, Klinikum Karlsburg, Karlsburg, Germany
    Pancreas 36:274-8. 2008
    On the basis of very low fecal elastase 1 and very high fecal fat estimations, it has been claimed that exocrine pancreatic insufficiency is frequent in diabetic patients, and that in up to 40% of the patients, pancreatic enzyme ..
  3. ncbi Pancreatic exocrine dysfunction in maturity-onset diabetes of the young type 3
    Mette Vesterhus
    Department of Pediatrics, Haukeland University Hospital, Bergen, Norway
    Diabetes Care 31:306-10. 2008
    ..We wanted to investigate the prevalence of pancreatic exocrine dysfunction in patients with maturity-onset diabetes of the young type 3 (MODY3)...
  4. ncbi The elimination of meat from the diet selectively decreases pancreatic elastase secretion
    Jaroslaw Walkowiak
    Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, Poznan, Poland
    Br J Nutr 98:154-8. 2007
    ..It was concluded that the exclusion of meat from the diet for a 1-month period results in significant changes in pancreatic secretion with a selective decrease of elastase-1 output. However, the underlying factor remains unclear...
  5. ncbi Pancreatin therapy in patients with insulin-treated diabetes mellitus and exocrine pancreatic insufficiency according to low fecal elastase 1 concentrations. Results of a prospective multi-centre trial
    Nils Ewald
    Third Medical Department and Policlinic, Giessen University Hospital, Giessen, Germany
    Diabetes Metab Res Rev 23:386-91. 2007
    Recently, high prevalence of exocrine dysfunction in diabetic populations has been reported. Patients with fecal elastase 1 concentration (FEC) <100 microg/g have also been demonstrated to suffer from steatorrhea in about 60% of cases, ..
  6. ncbi Evaluation of fecal pancreatic elastase-1 as a measure of pancreatic exocrine function in children with cystic fibrosis
    A Cade
    Regional Paediatric Cystic Fibrosis Unit, St James s University Hospital, Leeds, UK
    Pediatr Pulmonol 29:172-6. 2000
    ..The low values in the first 2 weeks in some normal and premature infants, and the persisting normal values in PS infants, make the fecal EL-1 test unsuitable for neonatal CF screening...
  7. ncbi Fecal elastase 1 and vitamin D3 in patients with osteoporotic bone fractures
    Sacha T W Mann
    Department of Internal Medicine, Medical Clinic III and Polyclinic of the Justus Liebig University, Giessen, Germany
    Eur J Med Res 13:68-72. 2008
    ..to clarify if patients with osteoporotic bone fractures have exocrine pancreatic insufficiency, especially reduced fecal elastase 1, connected with lowered serum levels of vitamin D3 that could be relevant for predominant osteoporosis.
  8. ncbi Pancreatic elastase-1 in stools, a marker of exocrine pancreas function, correlates with both residual beta-cell secretion and metabolic control in type 1 diabetic subjects
    Franco Cavalot
    University of Turin, San Luigi Gonzaga Hospital, Department of Clinical and Biological Sciences, Diabetes Unit, 10043 Orbassano TO, Turin, Italy
    Diabetes Care 27:2052-4. 2004
  9. ncbi Parathormone levels and Vitamin D metabolism in female patients with various grades of fecal elastase 1 deficiency
    J Teichmann
    Department of Internal Medicine, Medical Clinc C, Ludwigshafen, Germany
    Eur J Med Res 13:563-7. 2008
    ..still too few conclusive reports about conspicuous parathormone (PTH) and Vitamin D metabolism in patients with fecal elastase 1 deficiency or any connection of the calcium metabolism to the severity of exocrine pancreas insufficiency.
  10. ncbi Faecal pancreatic elastase--1 a non invasive measure of exocrine pancreatic function
    S D Amanquah
    Department of Chemical Pathology, University of Ghana Medical School, Accra, Ghana
    West Afr J Med 23:240-4. 2004
    ..Pancreatic elastase-1, is released into blood circulation during inflammation of the pancreas, but unlike most pancreatic enzymes it is stable during intestinal passage and not degraded...
  11. ncbi Now that fecal elastase is available in the United States, should clinicians start using it?
    Paul G Lankisch
    Clinic of Internal Medicine, Municipal Clinic of Lueneburg, Boegelstrasse 1, D 21339 Lueneburg, Germany
    Curr Gastroenterol Rep 6:126-31. 2004
    ..The test cannot be used in non-formed stool (ie, diarrhea) unless the stool is lyophilized. All in all, this new test does not seem to be the gold standard for pancreatic function that we have been waiting for...
  12. pmc Does an association exist between chronic pancreatitis and liver cirrhosis in alcoholic subjects?
    Luis Aparisi
    Liver Unit, Hospital Clinico Universitario, Avda Blasco Ibanez 17, Valencia 46010, Spain
    World J Gastroenterol 14:6171-9. 2008
    ..To study the possible association between chronic pancreatitis (CP) and liver cirrhosis (LC) of alcoholic etiology, after excluding any other causes...
  13. ncbi Comparing the urinary pancreolauryl ratio and faecal elastase-1 as indicators of pancreatic insufficiency in clinical practice
    D A Elphick
    Department of Gastroenterology, Barnsley District General Hospital, Barnsley, UK
    Pancreatology 5:196-200. 2005
    ..Both tests have previously been validated against 'gold standard' tests of pancreatic function, but their use in a clinical setting has never been directly compared...
  14. ncbi Faecal elastase 1: a marker of exocrine pancreatic insufficiency in cystic fibrosis
    I J Phillips
    Department of Chemical Pathology NHS, Southampton General Hospital, UK
    Ann Clin Biochem 36:739-42. 1999
    b>Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients...
  15. ncbi Early decline of pancreatic function in cystic fibrosis patients with class 1 or 2 CFTR mutations
    Jaroslaw Walkowiak
    Institute of Pediatrics, Department of Gastroenterology and Metabolism, Karol Marcinkowski University of Medical Sciences, 60 572 Poznan, Poland
    J Pediatr Gastroenterol Nutr 40:199-201. 2005
    ..We assessed the decline of pancreatic function in CF infants carrying class 1 or 2 CFTR mutations who were diagnosed in a neonatal screening program...
  16. pmc Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells
    Gerald Misinzo
    Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    J Virol 82:1128-35. 2008
    ..These results suggest that serine protease-mediated PCV2 disassembly is enhanced in porcine epithelial cells but inhibited in monocytic cells after inhibition of endosome-lysosome system acidification...
  17. ncbi Reappraisal of a method of reconstruction after pancreatoduodenectomy
    N J Lygidakis
    Department of Surgical Oncology, Henry Dunant Hospital, Athens, Greece
    Hepatogastroenterology 52:1077-82. 2005
    ..The present study is designed to substantiate the claims of various advantages of this method of PD and reconstruction and to explain the rationale behind this method...
  18. doi Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: Assessment versus an established assay and implication in classifying pancreatic function
    J Alan Erickson
    ARUP Laboratories, ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    Clin Chim Acta 397:87-91. 2008
    ..Disagreement continues regarding 2 fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function...
  19. doi Validation of fecal elastase-1 determination using immunoenzymatic assay in HIV-infected patients
    Claudia Giorgia Bronzatti de Oliveira
    Faculdade de Medicina do ABC, Sao Paulo, Brazil
    J Clin Lab Anal 22:286-90. 2008
    ..424). This test is useful to diagnose pathologies related to pancreatic insufficiency...
  20. ncbi [Biochemical diagnostics in acute pancreatitis recognition and outcome predicition]
    Paweł Olczyk
    Katedra i Zakład Chemii Klinicznej i Diagnostyki Laboratoryjnej, Slaska Akademia Medyczna, Sosnowiec
    Przegl Lek 61:1420-7. 2004
    ..All these newly introduced biochemical methods allow to look optimistically into the future of laboratory diagnostics of the acute pancreatitis believing that the problem of diagnosing and predicting the AP severity will be solved...
  21. ncbi Primary structure of human pancreatic elastase 2 determined by sequence analysis of the cloned mRNA
    T S Fletcher
    Biochemistry Research Laboratory, Veterans Administration Medical Center, Martinez, California 94553
    Biochemistry 26:7256-61. 1987
    ..pocket is bounded by Gly-216 and Ser-226, making this pocket intermediate in size between chymotrypsins and elastase 1 or protease E, consistent with the substrate specificity of elastase 2 for long-chain aliphatic or aromatic ..
  22. ncbi Diagnostic value of pancreatic elastase-1 in human acute pancreatitis
    Urszula Wereszczynska-Siemiatkowska
    Department of Gastroenterology, Medical University, Białystok, Poland
    Arch Immunol Ther Exp (Warsz) 51:195-200. 2003
    ..We suggest that pEla-1 has little diagnostic value and does not provide additional information to that of the less expensive and more widely available serum amylase and lipase...
  23. pmc Markers for trans-Golgi membranes and the intermediate compartment localize to induced membranes with distinct replication functions in flavivirus-infected cells
    J M MacKenzie
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston, Brisbane, Australia 4029
    J Virol 73:9555-67. 1999
    ..This is the first report identifying trans-Golgi membranes and the intermediate compartment as the apparent sources of the flavivirus-induced membranes involved in events of replication...
  24. pmc Low levels of pancreatic elastase 1 in stools of preterm infants
    F Campeotto
    Service de Neonatologie, Hôf pital Cochin Saint Vincent de Paul, 82 avenue Denfert Rochereau, 75674 Paris Cedex 14, France
    Arch Dis Child Fetal Neonatal Ed 86:F198-9. 2002
    The amount of faecal pancreatic enzyme elastase 1 was significantly lower in 42 preterm newborns than in 12 full term babies at day 2 (89 (3-539) v 354 (52-600) microg/g, p<0...
  25. ncbi Efficacy of oral pancreatic enzyme therapy for the treatment of fat malabsorption in HIV-infected patients
    A Carroccio
    Internal Medicine, University Hospital of Palermo, via Coffaro 25, 90124 Palermo, Italy
    Aliment Pharmacol Ther 15:1619-25. 2001
    ..Nutrient malabsorption is a negative prognostic factor in acquired immunodeficiency syndrome and recent studies have shown that pancreatic insufficiency is a codetermining factor of malabsorption...
  26. ncbi Exocrine pancreas disfunction in severely traumatised patients and early enteral nutrition
    Metin Senkal
    Department of General Surgery, Marien Hospital Witten, Germany
    Ulus Travma Acil Cerrahi Derg 14:34-9. 2008
    ..We investigated exocrine pancreatic insufficiency in severely traumatised patients with enteral nutrition using the fecal elastase-1 concentration...
  27. ncbi Physical mapping of the human ELA1 gene between D12S361 and D12S347 on chromosome 12q13
    R L Davies
    Department of Biology, Sweet Briar College, Virginia 24595, USA
    Genomics 29:766-8. 1995
    ELA1, the pancreatic elastase 1 gene, is conserved in mammalian genomes. ELA1 was previously mapped to chromosome 12 using a panel of mouse-human somatic cell hybrids...
  28. ncbi Association between steatorrhea, growth, and immunologic status in children with perinatally acquired HIV infection
    T A Sentongo
    Division of Gastroenterology, Hepatology, and Nutrition, Children s Memorial Medical Center, 2300 Children s Plaza No 65, Chicago, IL 60614
    Arch Pediatr Adolesc Med 155:149-53. 2001
    ..To examine the prevalence of steatorrhea and exocrine pancreatic insufficiency (EPI) and their association with growth and immune status variables in children with perinatally acquired human immunodeficiency virus (HIV) infection...
  29. pmc Chymotrypsin C (caldecrin) promotes degradation of human cationic trypsin: identity with Rinderknecht's enzyme Y
    Richard Szmola
    Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 104:11227-32. 2007
    ..in promoting trypsin degradation, because chymotrypsin B1, chymotrypsin B2, elastase 2A, elastase 3A, or elastase 3B are ineffective...
  30. ncbi Fecal elastase-1 is decreased in villous atrophy regardless of the underlying disease
    J Walkowiak
    Karol Marcinkowski University of Medical Sciences, Poznan, Poland
    Eur J Clin Invest 31:425-30. 2001
    ..E1 levels in other malabsorption conditions are unknown. Therefore, the aim of the present study was to evaluate E1 concentrations in various malabsorption syndromes...
  31. ncbi Exocrine pancreatic function in biliary tract pathology treated with the endoscopic methods
    J Wasielica-Berger
    Department of Gastroenterology and Internal Medicine, Medical University of Białystok, Poland
    Adv Med Sci 52:222-7. 2007
    ..The objective was to assess pancreatic exocrine function in biliary tract pathology (cholelithiasis, strictures) before and after endoscopic treatment...
  32. ncbi Exocrine pancreatic function in patients with end-stage renal disease
    J Griesche-Philippi
    Practice for Nephrology and Dialysis, University of Gottingen, Gottingen, Germany
    Clin Nephrol 74:457-64. 2010
    ..Our goal was therefore not only to establish the frequency but also the severity of exocrine pancreatic insufficiency in hemodialysis patients...
  33. doi Pancreatic acinar-specific overexpression of Reg2 gene offered no protection against either experimental diabetes or pancreatitis in mice
    Bing Li
    Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada
    Am J Physiol Gastrointest Liver Physiol 299:G413-21. 2010
    ..In contrast to INGAP or Reg3beta, exocrine overexpression of Reg2 offered no protection to the endocrine or exocrine pancreas, indicating clear subtype specificities of the Reg family of proteins...
  34. ncbi Exocrine pancreatic function during the early recovery phase of acute pancreatitis
    Raffaele Pezzilli
    Department of Digestive Diseases and Internal Medicine, University of Bologna, Sant Orsola Malpighi Hospital, Bologna, Italy
    Hepatobiliary Pancreat Dis Int 8:316-9. 2009
    ..This study aimed to prospectively assess the exocrine pancreatic function in patients with acute pancreatitis at the time of their refeeding...
  35. ncbi Autolysis of proproteinase E in bovine procarboxypeptidase A ternary complex gives rise to subunit III
    R Pascual
    Departament de Bioquimica Fac Ciències, Universitat Autonoma de Barcelona, Spain
    FEBS Lett 277:37-41. 1990
    ..residues when the former is allowed to autolyze in solution or when catalytic amounts of isolated active proteinase E are added to it...
  36. pmc In vitro antiviral activity and single-dose pharmacokinetics in humans of a novel, orally bioavailable inhibitor of human rhinovirus 3C protease
    Amy K Patick
    Department of Virology, Pfizer Global Research and Development, 10777 Science Center Drive, San Diego, CA 92121, USA
    Antimicrob Agents Chemother 49:2267-75. 2005
    ..These experiments highlight Compound 1 as a potent, orally bioavailable, irreversible inhibitor of HRV 3C protease and provide data that suggest that Cmax rather than the Cmin might be the key variable predicting clinical efficacy...
  37. ncbi Further studies on the human pancreatic binary complexes involving procarboxypeptidase A
    M Moulard
    Centre de Biochimie et de Biologie Moleculaire du CNRS, Marseille, France
    FEBS Lett 261:179-83. 1990
    ..previously reported, two of these complexes associated procarboxypeptidase A to either a glycosylated truncated protease E or zymogen E...
  38. doi Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats
    Hajime Tanaka
    Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
    Cancer Sci 101:341-6. 2010
    ..In marked contrast, injection of Ad-CAG-Cre resulted in pancreatic cancer development within 4 weeks. These results indicate that adult acinar cells are refractory to Ras oncogene activation and do not develop neoplasia in this model...
  39. pmc Crystal structure of bovine procarboxypeptidase A-S6 subunit III, a highly structured truncated zymogen E
    D Pignol
    Laboratoire de Cristallographie et de Cristallogénès des Protéines, Institut de Biologie Structurale, Grenoble, France
    EMBO J 13:1763-71. 1994
    ..Comparisons with the structures of porcine elastase 1 and chymotrypsinogen A indicate that the specific binding pocket of subunit III adopts a zymogen-like ..
  40. doi Usefulness of a 13C-labeled mixed triglyceride breath test for assessing pancreatic exocrine function after pancreatic surgery
    Hiroyuki Nakamura
    Department of Surgery, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    Surgery 145:168-75. 2009
    ..We evaluated the usefulness of a (13)C-labeled mixed triglyceride breath test for assessing pancreatic exocrine function after pancreatic surgery...
  41. doi Some patients with irritable bowel syndrome may have exocrine pancreatic insufficiency
    John S Leeds
    Department of Gastroenterology, Royal Hallamshire Hospital, Sheffield, United Kingdom
    Clin Gastroenterol Hepatol 8:433-8. 2010
    ..We evaluated the prevalence of exocrine pancreatic insufficiency in diarrhea-predominant IBS (D-IBS) and assessed the effects of pancreatic enzyme supplementation...
  42. doi Pancreatic fibrosis correlates with exocrine pancreatic insufficiency after pancreatoduodenectomy
    T C K Tran
    Department of Surgery, Erasmus Medical Center, Rotterdam, The Netherlands
    Dig Surg 25:311-8. 2008
    ..Obstruction of the pancreatic duct can lead to pancreatic fibrosis. We investigated the correlation between the extent of pancreatic fibrosis and the postoperative exocrine and endocrine pancreatic function...
  43. pmc Determinants of chymotrypsin C cleavage specificity in the calcium-binding loop of human cationic trypsinogen
    Andras Szabo
    Department of Molecular and Cell Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, MA 02118, USA
    FEBS J 279:4283-92. 2012
    ..highly specific, and other human pancreatic chymotrypsins (CTRB1, CTRB2 and CTRL1) and elastases (ELA2A, ELA3A and ELA3B) do not catalyze this reaction...
  44. ncbi Vitamin D3 in patients with various grades of chronic pancreatitis, according to morphological and functional criteria of the pancreas
    S T W Mann
    Department of Internal Medicine, Medical Clinic III and Polyclinic of the Justus Liebig University Giessen, Germany
    Dig Dis Sci 48:533-8. 2003
    ..inflammatory pancreas destruction (Cambridge classification), exocrine insufficiency (fecal elastase 1), and perhaps even the characteristics of sterol-binding of pancreatic elastase 1, which seems to be relevant for vitamin D supply.
  45. ncbi Pancreatic elastase-1 in stools, a marker of exocrine pancreas function, correlates with both residual beta-cell secretion and metabolic control in type 1 diabetic subjects: response to Mueller et al
    Franco Cavalot
    Diabetes Care 28:2810-1. 2005
  46. doi Protracted maturation of pancreatic-specific elastase 1 excretion in preterm infants of extremely low gestational age
    Annette Münch
    Department of Neonatology, Charite University Medical Center, Berlin, Germany
    J Pediatr Gastroenterol Nutr 56:532-6. 2013
    ..The aim of the present study was to better understand the exocrine pancreatic function of extremely preterm infants...
  47. doi Pancreatic exocrine function in patients with diabetes
    E Larger
    Laboratoire de Coprologie, Hôtpital de la Pitié Salpétrière, APHP, Paris, France
    Diabet Med 29:1047-54. 2012
    ..Decreased function of the exocrine pancreas is frequent in patients with diabetes. Our aim was to investigate clinical correlates of pancreatic exocrine failure in patients with diabetes...
  48. ncbi Display and release of the Plasmodium falciparum circumsporozoite protein using the autotransporter MisL of Salmonella enterica
    Patricia Ruiz-Olvera
    Unidad de Investigación Médica en Inmunología e Infectología, Hospital de Infectología Dr Daniel Méndez Hernández, Centro Medico La Raza, IMSS, D F, Mexico
    Plasmid 50:12-27. 2003
    ..These results demonstrate that MisL may be used for the autodisplay and release of passenger proteins in attenuated Salmonella or E. coli strains, which may have several applications in vaccine design...
  49. doi Comparison of fecal elastase-1 and pancreatic function testing in children
    Prateek D Wali
    Department of Pediatrics, Thomas Jefferson University, Philadelphia, PA, USA
    J Pediatr Gastroenterol Nutr 54:277-80. 2012
    ..We aimed to evaluate the performance of the FE-1 test compared with the direct pancreatic function test (PFT) with secretin stimulation in children...
  50. ncbi A new fecal elastase 1 test using polyclonal antibodies for the detection of exocrine pancreatic insufficiency
    Jan Uwe Hahn
    Pancreas 30:189-91. 2005
  51. pmc Quantification of pancreatic exocrine function of chronic pancreatitis with secretin-enhanced MRCP
    Yun Bian
    Yun Bian, Li Wang, Chao Chen, Jian Ping Lu, Jia Bao Fan, Shi Yue Chen, Bing Hui Zhao, Department of Radiology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China
    World J Gastroenterol 19:7177-82. 2013
    ....
  52. ncbi [The role of fecal elastase-1 in pancreatic diseases]
    Xiao Ou Yang
    Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China
    Zhonghua Nei Ke Za Zhi 45:285-8. 2006
    ....
  53. ncbi Evaluating the role of serum elastase 1 in the diagnosis of pancreatic cancer
    Dong Wu
    Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China
    Chin J Dig Dis 7:117-20. 2006
    To determine the normal value of serum elastase 1 in Chinese adults and evaluate its diagnostic value for pancreatic cancer.
  54. ncbi Evaluation of serum values of pancreatic enzymes after endoscopic retrograde pancreatography in dogs
    Thomas Spillmann
    Department of Small Animal Internal Medicine at the Medical and Forensic Veterinary Clinic I, Justus Liebig University Giessen, Frankfurter Strasse 126, 35392 Giessen, Germany
    Am J Vet Res 65:616-9. 2004
    ..pancreatography (ERP) in dogs by performing repeated clinical examinations and laboratory analyses of serum amylase, lipase, canine trypsin-like immunoreactivity (cTLI), and canine pancreatic elastase 1 (cE1) after the procedure.
  55. ncbi Screening for pancreatic exocrine insufficiency in patients with diabetes mellitus
    Amadeu C R Nunes
    Department of Gastroenterology, University Hospitals of Coimbra, Avenida Urbano Duarte, Quinta da Estrela, Lote 7, 6o B, Coimbra, Portugal
    Am J Gastroenterol 98:2672-5. 2003
    b>Fecal elastase 1 (E1) is a relatively sensitive and specific indirect test of pancreatic exocrine function...
  56. ncbi High prevalence of steatorrhea in 101 diabetic patients likely to suffer from exocrine pancreatic insufficiency according to low fecal elastase 1 concentrations: a prospective multicenter study
    Philip D Hardt
    Third Medical Department and Policlinic, Giessen University Hospital, Giessen, Germany
    Dig Dis Sci 48:1688-92. 2003
    ..fecal fat excretion in patients with type 1 or type 2 diabetes mellitus and exocrine dysfunction according to fecal elastase 1 concentrations <100 microg/g was investigated...
  57. ncbi Fecal elastase-1 determination: 'gold standard' of indirect pancreatic function tests?
    S Luth
    Dept of Medicine II Gastroenterology Hepatology, University Hospital of Heidelberg at Mannheim, Germany
    Scand J Gastroenterol 36:1092-9. 2001
    ..The aim of our study was to re-evaluate the sensitivity and specificity of elastase-1 and chymotrypsin in stool in the assessment of exocrine pancreatic insufficiency...
  58. ncbi Prt-4 and Prt-5: new constituents of a gene cluster on chromosome 7 coding for esterproteases in the submandibular gland of the house mouse (Mus musculus)
    J Otto
    Biochem Genet 19:431-44. 1981
    ..for two different enzymes with esterproteolytic activity from mouse submandibular gland: protease A and protease E. Based upon strain distribution and biochemical properties of the two esterproteases, the existence of two ..
  59. ncbi Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase
    N K Honey
    Somat Cell Mol Genet 10:369-76. 1984
    ..TRY1 was assigned to human chromosome 7q22----qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes...
  60. ncbi [Fecal elastase-1 test: clinical evaluation of a new noninvasive pancreatic function test]
    Masahiro Takeda
    Central Clinical Laboratories, Tokyo Women s Medical University, Tokyo 162 8666
    Rinsho Byori 50:893-8. 2002
    ..We evaluated clinical significance of fecal elastase-1(FE-1) in patients with pancreatic diseases and pancreatic dysfunction, in comparison with other tubeless test and secretin test...
  61. doi Magnetic resonance imaging and magnetic resonance cholangiopancreatography findings compared with fecal elastase 1 measurement for the diagnosis of chronic pancreatitis
    Mehmet Bilgin
    Department of Radiology, University Hospital Giessen and Marburg, Giessen, Germany
    Pancreas 36:e33-9. 2008
    To correlate magnetic resonance cholangiopancreatography (MRCP) and magnetic resonance imaging (MRI) findings of the pancreas with the pancreatic exocrine function determined by fecal elastase 1 concentration.
  62. doi Pancreatic exocrine insufficiency is not common in HNF-1alpha MODY
    T Klupa
    Department of Metabolic Diseases, Jagiellonian University, Medical College, Krakow, Poland
    Diabet Med 25:627-30. 2008
    ..The aim of this study was to determine the prevalence of exocrine pancreas dysfunction in HNF-1alpha MODY patients...
  63. ncbi Normal pancreatic secretion in children with progressive familial intrahepatic cholestasis type 1
    Jaroslaw Walkowiak
    Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, Poznan, Poland
    Scand J Gastroenterol 41:1480-3. 2006
    ..Therefore, the objective of this study was to assess exocrine pancreatic function in patients with PFIC1...
  64. pmc The hepatitis C virus replicon presents a higher barrier to resistance to nucleoside analogs than to nonnucleoside polymerase or protease inhibitors
    Matthew F McCown
    Department of HCV Biology, Virology Disease Biology Area, Roche Palo Alto LLC, 3431 Hillview Ave, Palo Alto, CA 94304, USA
    Antimicrob Agents Chemother 52:1604-12. 2008
    ..Furthermore, the combination of a nonnucleoside or protease inhibitor with a nucleoside polymerase inhibitor could have a clear clinical benefit through the delay of resistance emergence...
  65. ncbi Correlation between male fertility and acrosin-like protease activity in rats treated with Spartium junceum
    J S Chen
    Centro Studio Cellule Germinali del CNR, University of Siena, Italy
    Zygote 1:309-13. 1993
    ..The implications of this finding is discussed although the exact molecular basis of the phenomenon is uncertain...
  66. pmc Pharmacological activities of TEI-8362, a novel inhibitor of human neutrophil elastase
    H Mitsuhashi
    Teijin Institute for Bio Medical Research, Hino, Tokyo, Japan
    Br J Pharmacol 126:1147-52. 1999
    ..t., i.v., or inhalant administration of TEI-8362. 5. These pharmacological actions of TEI-8362 suggest that this drug has therapeutic value in the treatment of destructive lung diseases due to neutrophils...
  67. ncbi Human elastase 1: evidence for expression in the skin and the identification of a frequent frameshift polymorphism
    U Talas
    Center for Cutaneous Research, St Bartholomew s and the Royal London School of Medicine and Dentistry, Whitechapel, London, UK
    J Invest Dermatol 114:165-70. 2000
    Human pancreatic elastase 1 is a serine protease which maps to the chromosomal region 12q13 close to a locus for an autosomal dominant skin disease, diffuse nonepidermolytic palmoplantar keratoderma, and was investigated as a possible ..
  68. ncbi Alterations of vitamin D3 metabolism in young women with various grades of chronic pancreatitis
    J Teichmann
    Department of Internal Medicine, Medical Clinic C, Ludwigshafen, Germany
    Eur J Med Res 12:347-50. 2007
    ..Therefore the aim of this study was to examine marker of vitamin D3 metabolism in female patients with episode of biliary pancreatitis to determine if increased severity of the disease would correlate with impaired vitamin D3 metabolism...
  69. ncbi Biliary versus alcohol-related infected pancreatic necrosis: similarities and differences in the follow-up
    Jacek Reszetow
    Department of General, Endocrine, and Transplant Surgery, Medical University of Gdansk, Poland
    Pancreas 35:267-72. 2007
    ..Data concerning survivors' quality of life and pancreatic functions are scarce. Follow-up of the patients with alcohol and biliary etiology of IPN treated with open necrosectomy was performed...
  70. ncbi Fecal calprotectin and elastase 1 determinations in patients with pancreatic diseases: a possible link between pancreatic insufficiency and intestinal inflammation
    Raffaele Pezzilli
    Department of Digestive Diseases and Internal Medicine, Sant Orsola Malpighi Hospital, University of Bologna, Via Massarenti 9, 40138, Bologna, Italy
    J Gastroenterol 42:754-60. 2007
    ..Our aim was to assess fecal calprotectin in order to evaluate the presence of intestinal inflammation in patients with pancreatic disease...
  71. ncbi Behaviour of serum pancreatic enzymes in chronic pancreatitis
    R Pezzilli
    Emergency Department, Sant Orsola Hospital, Bologna, Italy
    Dig Liver Dis 32:233-7. 2000
    ..To establish whether serum pancreatic enzyme determination is useful in the identification of patients with chronic pancreatitis and in revealing the presence of exocrine pancreatic insufficiency..
  72. doi Increased expression of Smad6 deteriorates murine acute experimental pancreatitis in two models
    H Nakamura
    Third Department of Internal Medicine, University of Occupational and Environmental Health, Japan, School of Medicine, 1 1 Iseigaoka, Yahatanishi ku Kitakyushu 807 8555, Japan
    Gut 57:788-98. 2008
    ..Smad6 is implicated in the inhibition of bone morphogenetic protein signalling. However, the function of Smad6 in the pancreas remains obscure...
  73. ncbi Interaction between human pancreatic elastase and plasma protease inhibitors
    E L Gustavsson
    Hoppe Seylers Z Physiol Chem 361:169-76. 1980
    ..The elastase bound by alpha 2-macroglobulin retains its activity against low molecular weight substrates, while that bound by alpha 1-antitrypsin is enzymologically inactive...
  74. doi MRI and MRCP findings of the pancreas in patients with diabetes mellitus: compared analysis with pancreatic exocrine function determined by fecal elastase 1
    Mehmet Bilgin
    Department of Radiology, University Hospital Giessen and Marburg, Giessen, Germany
    J Clin Gastroenterol 43:165-70. 2009
    ....
  75. ncbi Significance of increased serum elastase 1 level during the hyperosmolar hyperglycemic non-ketotic syndrome
    S Umeki
    Department of Medicine, Kawasaki Medical School, Okayama, Japan
    Hepatogastroenterology 34:212-4. 1987
    An alteration in the serum elastase 1 level in a previously non-diabetic patient, who unfortunately developed the hyperosmolar hyperglycemic non-ketotic syndrome (HHNS), was observed after intravenous hyperalimentation for 6 days...
  76. ncbi Effects of elastase-induced emphysema on airway responsiveness to methacholine in rats
    S Bellofiore
    Meakins Christie Laboratories, McGill University, Montreal, Quebec, Canada
    J Appl Physiol (1985) 66:606-12. 1989
    ..56 ml (+/- 0.08 ml) significantly reduced RL at 64 mg MCh/ml from 0.62 +/- 0.05 cmH2O.ml-1.s to 0.50 +/- 0.03, P less than 0.05.(ABSTRACT TRUNCATED AT 250 WORDS)..
  77. ncbi Generation of a subunit III-like protein by autolysis of human and porcine proproteinase e in a binary complex with procarboxypeptidase A
    F X Aviles
    Departament de Bioquimica í Biologia Molecular Facultat de Ciències, Universitat Autonoma de Barcelona, Spain
    Biochem Biophys Res Commun 163:1191-6. 1989
    ..treatment of human and porcine proproteinase E, procarboxypeptidase A binary complexes gave rise to active proteinase E after removal of an 11-residue N-terminal activation peptide...
  78. ncbi Normal levels of serum pancreatic enzymes in patients with progressive familial intrahepatic cholestasis type 2
    Jaroslaw Walkowiak
    Poznan University of Medical Sciences, Department of Pediatric Gastroenterology and Metabolism, Poland
    Acta Biochim Pol 57:573-5. 2010
    ..However, this state is related predominantly to biliary atresia. Therefore, we aimed to assess pancreatic damage by measuring serum pancreatic enzymes in patients with progressive familial intrahepatic cholestasis type 2 (PFIC type 2)...
  79. ncbi Substitution of valine for glycine-558 in the congenital dysthrombin thrombin Quick II alters primary substrate specificity
    R A Henriksen
    Department of Biochemistry, University of Vermont, Burlington 05405
    Biochemistry 28:2078-82. 1989
    ..However, in porcine elastase 1, the corresponding residue is threonine...
  80. ncbi Identification of pancreatic juice proteins as biomarkers of pancreatic cancer
    Jun Gao
    Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China
    Oncol Rep 23:1683-92. 2010
    ..lipase-related protein-1 (PLRP1), and two proteins were down-regulated, chymotrypsinogen B (CTRB) precursor and elastase 3B (ELA3B) preproprotein...
  81. doi Pancreatic exocrine function is preserved after distal pancreatectomy
    James E Speicher
    Department of General Surgery, Virginia Mason Medical Center, Seattle, WA 98111, USA
    J Gastrointest Surg 14:1006-11. 2010
    ..Our objective was to measure human stool elastase-1 to determine the effect of distal pancreatectomy on exocrine function...
  82. doi Functional changes after pancreatoduodenectomy: diagnosis and treatment
    T C Khe Tran
    Department of Surgery, Erasmus Medical Center, Rotterdam, The Netherlands
    Pancreatology 9:729-37. 2009
    ..Furthermore, the functional changes after pancreatoduodenectomy are described in detail with suggestions for diagnosis and treatment...
  83. pmc Recombinant derivatives of botulinum neurotoxin A engineered for trafficking studies and neuronal delivery
    Philip A Band
    Department of Pharmacology, New York University School of Medicine, New York, NY 10016, United States
    Protein Expr Purif 71:62-73. 2010
    ..Moreover, they create the potential to engineer BoNT-based molecular vehicles that can target therapeutic agents to the neuronal cytoplasm...
  84. pmc Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas
    M Cockell
    Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland
    Mol Cell Biol 9:2464-76. 1989
    ..vitro binding assays that this DNA-binding activity also recognizes a sequence within the 5'-flanking regions of elastase 1, chymotrypsinogen B, carboxypeptidase A, and trypsind genes...
  85. ncbi Molecular cloning of complementary DNA for human medullasin: an inflammatory serine protease in bone marrow cells
    K Okano
    Basic Research Laboratories, Toray Industries, Inc, Kanagawa
    J Biochem 102:13-6. 1987
    ..The deduced amino acid sequence of medullasin shows a typical serine protease structure, with 41% homology with pig elastase 1.
  86. doi A study of pancreatic function among subjects over ninety years of age
    Lucio Gullo
    Institute of Internal Medicine, University of Bologna, St Orsola Hospital, Bologna, Italy
    Pancreatology 9:240-4. 2009
    ..Among the various studies of pancreatic function in the elderly published so far, none have dealt with subjects over 90 years of age. The aim of this study was to examine pancreatic function in healthy individuals over 90 years old...
  87. ncbi Noncatalytic protein component of elastase from horse leucocytes. A protein with regulatory function
    J Potempa
    Institute of Molecular Biology, Jagiellonian University, Cracow, Poland
    Int J Biochem 20:105-8. 1988
    ..5) was separated from native horse leucocyte elastase 1. 2...
  88. ncbi Prevalence of pancreatic insufficiency in inflammatory bowel diseases. Assessment by fecal elastase-1
    Giovanni Maconi
    Department of Clinical Sciences, L Sacco University Hospital, Via GB Grassi, 74, Milan, Italy
    Dig Dis Sci 53:262-70. 2008
    ..PI is reversible in most patients, and persistent PI is not associated with clinically active disease...
  89. ncbi Endoscopic sphincterotomy in patients with stenosis of ampulla of Vater: three-year follow-up of exocrine pancreatic function and clinical symptoms
    Nils Ewald
    Third Medical Department and Policlinic, University Hospital Giessen and Marburg, Giessen Site, D 35392 Giessen, Germany
    World J Gastroenterol 13:901-5. 2007
    ..To investigate retrospectively the long-term effect of endoscopic sphincterotomy (ES) including exocrine pancreatic function in patients with stenosis of ampulla of Vater...
  90. ncbi Prevalence and determinants of exocrine pancreatic insufficiency among older adults: results of a population-based study
    Dietrich Rothenbacher
    Department of Epidemiology, German Centre for Research on Ageing, University of Heidelberg, Heidelberg, Germany
    Scand J Gastroenterol 40:697-704. 2005
    ..The prevalence and main determinants of exocrine pancreatic insufficiency were investigated in a large population-based sample of older adults by measuring pancreatic elastase-1 in stool...
  91. ncbi Analysis of exocrine pancreatic function in cystic fibrosis: one mild CFTR mutation does not exclude pancreatic insufficiency
    J Walkowiak
    Karol Marcinkowski University of Medical Sciences, Poznan, Poland
    Eur J Clin Invest 31:796-801. 2001
    ..The aim of the present study is to evaluate the correlation between genotype and exocrine pancreatic insufficiency in CF patients. The special emphasis was put on the analysis of mild CFTR mutations...
  92. ncbi Pancreatic elastase induces liver injury by activating cytokine production within Kupffer cells via nuclear factor-Kappa B
    Michel M Murr
    Department of Surgery, James A Haley Veterans Hospital, University of South Florida, Tampa, FL, USA
    J Gastrointest Surg 6:474-80. 2002
    ..Gd exhibits a protective effect against elastase-induced liver injury by inhibiting activation of NF-kappa B...
  93. ncbi Fecal elastase-1 is superior to fecal chymotrypsin in the assessment of pancreatic involvement in cystic fibrosis
    Jaroslaw Walkowiak
    Institute of Pediatrics, Karol Marcinkowski University of Medical Sciences, Poznan, Poland
    Pediatrics 110:e7. 2002
    ..Therefore, the aim of the present study was to compare the sensitivity and the specificity of the fecal elastase-1 (E1) test with the fecal chymotrypsin (ChT) test in a large cohort of CF patients and healthy subjects (HS)...
  94. ncbi Faecal elastase-1 and fat-soluble vitamin profiles in patients with cystic fibrosis in Western Norway
    Ludger Dorlöchter
    Department of Paediatrics, Haukeland University Hospital, 5021 Bergen, Norway
    Eur J Nutr 41:148-52. 2002
    ..Human faecal elastase-1 (FE-1) has shown promising results to assess exocrine pancreatic insufficiency, and this test has been used at Haukeland University Hospital since 1996...
  95. ncbi [Immunomodulatory activity and anti-tumor activity of Oldenlandia diffusa in vitro]
    B E Shan
    Research Center 4th Hospital, Hebei Medical University, Shijiazhuang 050011
    Zhongguo Zhong Xi Yi Jie He Za Zhi 21:370-4. 2001
    ..To study the effect of Oldenlandia diffusa (OD) on lymphocytes and tumor cells in vitro...
  96. ncbi Longitudinal follow-up of exocrine pancreatic function in pancreatic sufficient cystic fibrosis patients using the fecal elastase-1 test
    Jaroslaw Walkowiak
    Department of Gastroenterology and Metabolism, Institute of Pediatrics, Karol Marcinkowski University of Medical Sciences, Szpitalna 27 33, 60 572 Poznan, Poland
    J Pediatr Gastroenterol Nutr 36:474-8. 2003
    ..Therefore, the aim of the present study was to evaluate the usefulness of the noninvasive fecal elastase-1 (E1) test for the longitudinal assessment of exocrine pancreatic function (EPF) in pancreatic-sufficient (PS) CF patients...
  97. ncbi Variability of fecal pancreatic elastase measurements in cystic fibrosis patients
    I Meyts
    Pediatric Department, University Hospital Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    J Cyst Fibros 1:265-8. 2002
    ..the fecal pancreatic elastase-1 (EL-1) test is a new non-invasive test for pancreatic function. The aim of the study was to evaluate the intra-patient variability of the fecal EL-1 test in a cystic fibrosis (CF) population...
  98. ncbi Fecal elastase-1 cut-off levels in the assessment of exocrine pancreatic function in cystic fibrosis
    Jaroslaw Walkowiak
    Institute of Pediatrics, Department of Gastroenterology and Metabolism, Karol Marcinkowski University of Medical Sciences, Szpitalna 27 33, 60 572 Poznan, Poland
    J Cyst Fibros 1:260-4. 2002
    ..Fecal elastase-1 (E1) test is a sensitive and specific indirect test. However, there are few data on the best cut-off level in the assessment of exocrine pancreatic function in cystic fibrosis (CF)...
  99. ncbi Faecal elastase-1 test is superior to faecal lipase test in the assessment of exocrine pancreatic function in cystic fibrosis
    J Walkowiak
    Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, Poznan, Poland
    Acta Paediatr 93:1042-5. 2004
    ..Therefore, the aim of the present study was to compare the sensitivity and the specificity of the faecal lipase test to the faecal elastase-1 test in the assessment of exocrine pancreatic function in children with CF...
  100. ncbi Use of fecal elastase-1 to classify pancreatic status in patients with cystic fibrosis
    Drucy Borowitz
    Department of Pediatrics, Women and Children s Hospital of Buffalo, State University of New York at Buffalo, 219 Bryant Street, Buffalo, NY 14222, USA
    J Pediatr 145:322-6. 2004
    ..To test the hypothesis that some patients with cystic fibrosis (CF) are misclassified as pancreatic insufficient, using fecal elastase-1 (FE-1) to define pancreatic status...
  101. ncbi The commercially available ELISA for pancreatic elastase 1 based on polyclonal antibodies does measure an as yet unknown antigen different from purified elastase 1. Binding studies and clinical use in patients with exocrine pancreatic insufficiency
    P D Hardt
    Third Medical Department and Policlinic, Giessen University Hospital, Giessen, Germany
    Z Gastroenterol 41:903-6. 2003
    The measurement of fecal elastase 1 concentrations by means of an ELISA based on monoclonal antibodies (mABs) highly specific for human elastase 1 (ELISA 1) has become an accepted indirect test of the exocrine pancreatic function during ..

Research Grants11

  1. Inflammatory response to pancreatitis in CF mice: sodium & impaired apoptosis
    MATTHEW DIMAGNO; Fiscal Year: 2007
    ..Understanding of the pathophysiology of AP is incomplete & therapies are lacking. The proposed study may provide insight into the pathogenesis of AP & CF & contribute to generation of novel therapies. ..