cdk9

Summary

Gene Symbol: cdk9
Description: cyclin-dependent kinase 9
Alias: C-2k, CDC2L4, CTK1, PITALRE, TAK, CDC2-related kinase, cell division cycle 2-like protein kinase 4, cell division protein kinase 9, serine/threonine protein kinase PITALRE, serine/threonine-protein kinase PITALRE, tat-associated kinase complex catalytic subunit
Species: human

Top Publications

  1. ncbi A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
    P Wei
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Cell 92:451-62. 1998
  2. ncbi SKIP interacts with c-Myc and Menin to promote HIV-1 Tat transactivation
    Vanessa Brès
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037 1099, USA
    Mol Cell 36:75-87. 2009
  3. ncbi 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes
    V T Nguyen
    Genetique Moleculaire, UMR 8541 CNRS, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Nature 414:322-5. 2001
  4. ncbi CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA
    M E Garber
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Mol Cell Biol 20:6958-69. 2000
  5. ncbi The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation
    Sonja Baumli
    Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 27:1907-18. 2008
  6. ncbi DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation
    Y H Ping
    Department of Pharmacology, Robert Wood Johnson Medical School, and Molecular Biosciences Graduate Program at Rutgers University, Piscataway, New Jersey 08854, USA
    J Biol Chem 276:12951-8. 2001
  7. ncbi Transcription elongation factor P-TEFb is required for HIV-1 tat transactivation in vitro
    Y Zhu
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USA
    Genes Dev 11:2622-32. 1997
  8. ncbi The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
    Z Yang
    Department of Molecular and Cell Biology, University of California at Berkeley, USA
    Nature 414:317-22. 2001
  9. ncbi The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention
    Miguel Stevens
    Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Med Res Rev 26:595-625. 2006
  10. ncbi Cooperative interaction between HIV-1 regulatory proteins Tat and Vpr modulates transcription of the viral genome
    B E Sawaya
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
    J Biol Chem 275:35209-14. 2000

Research Grants

  1. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2007
  2. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2009
  3. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2010
  4. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2009
  5. Tat Transactivation
    BORIS MATIJA PETERLIN; Fiscal Year: 2010
  6. Tat Transactivation
    B Matija Peterlin; Fiscal Year: 2007
  7. Tat Transactivation
    BORIS PETERLIN; Fiscal Year: 2004
  8. CYCLIN T AND HIV1 TAT TRANSACTIVATION
    Katherine Jones; Fiscal Year: 2007
  9. Structure and Function of Ribonucleoprotein Complexes Modulating HIV Replication
    Tariq M Rana; Fiscal Year: 2010
  10. IMPACT OF TUBERCULOSIS ON HIV DISEASE
    ZAHRA TOOSSI TOOSSI; Fiscal Year: 2010

Detail Information

Publications204 found, 100 shown here

  1. ncbi A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
    P Wei
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Cell 92:451-62. 1998
    ..Cyclin T is a partner for CDK9, an RNAPII transcription elongation factor...
  2. ncbi SKIP interacts with c-Myc and Menin to promote HIV-1 Tat transactivation
    Vanessa Brès
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037 1099, USA
    Mol Cell 36:75-87. 2009
    The Ski-interacting protein SKIP/SNW1 associates with the P-TEFb/CDK9 elongation factor and coactivates inducible genes, including HIV-1...
  3. ncbi 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes
    V T Nguyen
    Genetique Moleculaire, UMR 8541 CNRS, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Nature 414:322-5. 2001
    ..CTD) of the largest polymerase subunit by a transcription elongation factor, P-TEFb, which comprises the kinase CDK9 and cyclin T1 or T2 (ref. 1)...
  4. ncbi CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA
    M E Garber
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Mol Cell Biol 20:6958-69. 2000
    Human immunodeficiency virus type 1 (HIV-1) Tat interacts with cyclin T1 (CycT1), a regulatory partner of CDK9 in the positive transcription elongation factor (P-TEFb) complex, and binds cooperatively with CycT1 to TAR RNA to recruit P-..
  5. ncbi The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation
    Sonja Baumli
    Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 27:1907-18. 2008
    The positive transcription elongation factor b (P-TEFb) (CDK9/cyclin T (CycT)) promotes mRNA transcriptional elongation through phosphorylation of elongation repressors and RNA polymerase II...
  6. ncbi DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation
    Y H Ping
    Department of Pharmacology, Robert Wood Johnson Medical School, and Molecular Biosciences Graduate Program at Rutgers University, Piscataway, New Jersey 08854, USA
    J Biol Chem 276:12951-8. 2001
    ..These findings reveal a molecular mechanism for the negative and positive regulation of transcriptional elongation at the HIV-1 promoter...
  7. ncbi Transcription elongation factor P-TEFb is required for HIV-1 tat transactivation in vitro
    Y Zhu
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USA
    Genes Dev 11:2622-32. 1997
    ..Sequence comparison suggests that a protein with 72% identity, PITALRE, could be the human homolog of the Drosophila protein...
  8. ncbi The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
    Z Yang
    Department of Molecular and Cell Biology, University of California at Berkeley, USA
    Nature 414:317-22. 2001
    The human positive transcription elongation factor P-TEFb, consisting of a CDK9/cyclin T1 heterodimer, functions as both a general and an HIV-1 Tat-specific transcription factor...
  9. ncbi The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention
    Miguel Stevens
    Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Med Res Rev 26:595-625. 2006
    ..The complex of Tat with p-TEFb then binds the loop structures of TAR RNA thereby positioning CDK9 to phosphorylate the cellular RNA polymerase II...
  10. ncbi Cooperative interaction between HIV-1 regulatory proteins Tat and Vpr modulates transcription of the viral genome
    B E Sawaya
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
    J Biol Chem 275:35209-14. 2000
    ..Because Tat utilizes cyclin T1 and its partner, CDK9 to elevate the level of transcription from the LTR, we examined the cooperativity between Vpr, Tat, and cyclin T1/..
  11. ncbi Heat shock protein 40 is necessary for human immunodeficiency virus-1 Nef-mediated enhancement of viral gene expression and replication
    Manish Kumar
    National Centre for Cell Science, Ganeshkhind, Pune 411007, India
    J Biol Chem 280:40041-50. 2005
    ..Thus our results clearly indicate that Hsp40 is crucial for Nef-mediated enhancement of viral gene expression and replication...
  12. ncbi Multiple actions of the human immunodeficiency virus type-1 Tat protein on microglial cell functions
    Luisa Minghetti
    Department of Cell Biology and Neuroscience, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Neurochem Res 29:965-78. 2004
    ....
  13. ncbi A human splicing factor, SKIP, associates with P-TEFb and enhances transcription elongation by HIV-1 Tat
    Vanessa Brès
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Genes Dev 19:1211-26. 2005
    HIV-1 Tat binds human CyclinT1 and recruits the CDK9/P-TEFb complex to the viral TAR RNA in a step that links RNA polymerase II (RNAPII) C-terminal domain (CTD) Ser 2 phosphorylation with transcription elongation...
  14. ncbi Cyclin K inhibits HIV-1 gene expression and replication by interfering with cyclin-dependent kinase 9 (CDK9)-cyclin T1 interaction in Nef-dependent manner
    Sohrab Zafar Khan
    National Centre for Cell Science, Ganeshkhind, Pune 411007, India
    J Biol Chem 286:22943-54. 2011
    ..The positive elongation factor b complex comprising cyclin-dependent kinase 9 (CDK9) and Cyclin T1 is a critical cellular complex required for viral gene expression and replication...
  15. ncbi Inhibition of P-TEFb (CDK9/Cyclin T) kinase and RNA polymerase II transcription by the coordinated actions of HEXIM1 and 7SK snRNA
    Jasper H N Yik
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 12:971-82. 2003
    The positive transcriptional elongation factor b (P-TEFb), consisting of CDK9 and cyclin T, stimulates transcription by phosphorylating RNA polymerase II. It becomes inactivated when associated with the abundant 7SK snRNA...
  16. ncbi Controlling cellular P-TEFb activity by the HIV-1 transcriptional transactivator Tat
    Lisa Muniz
    Laboratoire de Biologie Moleculaire Eucaryote du CNRS, UMR5099, IFR109 CNRS, Universite Paul Sabatier, Toulouse, France
    PLoS Pathog 6:e1001152. 2010
    ....
  17. ncbi A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription
    C A Parada
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    EMBO J 18:3688-701. 1999
    ..Our results indicate that Tat-SF is a Tat cofactor-containing RNA Pol II complex whose recruitment to the promoter provides elongation factors important for Tat-enhanced HIV-1 transcription following TAR RNA synthesis...
  18. ncbi Induction of monocyte chemoattractant protein-1 (MCP-1/CCL2) gene expression by human immunodeficiency virus-1 Tat in human astrocytes is CDK9 dependent
    Abdelkader Khiati
    INSERM U802 and Université Paris Sud 11, Faculte de Medecine Paris Sud, Le Kremlin Bicetre, France
    J Neurovirol 16:150-67. 2010
    ..factor kappaB (NF-kappaB) classical pathway, but was significantly inhibited by specific cyclin-dependent kinase 9 (cdk9) inhibitors, such as a dominant-negative mutant or siRNA...
  19. ncbi Reciprocal modulation of transcriptional activities between HIV-1 Tat and MHC class II transactivator CIITA
    H Okamoto
    Department of Molecular Genetics, Nagoya City University Medical School, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya, Aichi, 467 8601, Japan
    Biochem Biophys Res Commun 279:494-9. 2000
    ..These reciprocal actions between Tat and CIITA not only explains the functional impairment of APC in HIV-1 infection but also rationalizes the suppression of HIV-1 virus load by induction of CIITA such as IFN-gamma...
  20. ncbi Regulation of human immunodeficiency virus type 1 gene expression by clade-specific Tat proteins
    Yan Desfosses
    McGill AIDS Center, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, 3755 Cote Ste Catherine, Montreal, Quebec, Canada H3T1E2
    J Virol 79:9180-91. 2005
    ..Swapping the activation domains of Tat B, C, and E identified the cyclin T1 association domain as a critical determinant of the transactivation efficiency and of Tat-defective HIV-1 provirus rescue...
  21. ncbi P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro
    H S Mancebo
    Tularik, Inc, South San Francisco, California 94080 USA
    Genes Dev 11:2633-44. 1997
    ..Loss of Tat transactivation in extracts depleted of the kinase subunit of human P-TEFb, PITALRE, was reversed by addition of partially purified human P-TEFb...
  22. ncbi Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation
    Young Kyeung Kim
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    Mol Cell Biol 22:4622-37. 2002
    Stimulation of transcriptional elongation by the human immunodeficiency virus type 1 Tat protein is mediated by CDK9, a kinase that phosphorylates the RNA polymerase II carboxyl-terminal domain (CTD)...
  23. ncbi Domains in the SPT5 protein that modulate its transcriptional regulatory properties
    D Ivanov
    Division of Hematology Oncology, Department of Medicine, Harold Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas 75235 8594, USA
    Mol Cell Biol 20:2970-83. 2000
    ..Recent data suggest that P-TEFb, which is composed of CDK9 and cyclin T1, is also critical in regulating transcriptional elongation by SPT4 and SPT5...
  24. ncbi MAQ1 and 7SK RNA interact with CDK9/cyclin T complexes in a transcription-dependent manner
    Annemieke A Michels
    UMR 8541 CNRS, Ecole Normale Superieure, Laboratoire de Régulation de l Expression Génétique, 75230 Paris Cedex 05, France
    Mol Cell Biol 23:4859-69. 2003
    ..transcription elongation factor b (P-TEFb) comprises a cyclin (T1 or T2) and a kinase, cyclin-dependent kinase 9 (CDK9), which phosphorylates the carboxyl-terminal domain of RNA polymerase II...
  25. ncbi HIV-1 infection and regulation of Tat function in macrophages
    Li Ying Liou
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Int J Biochem Cell Biol 36:1767-75. 2004
    ..The viral Tat protein, whose function is mediated by the cellular cyclin T1 protein complexed with CDK9, is required for efficient transcription of the integrated HIV-1 provirus by RNA polymerase II...
  26. ncbi MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein
    Mario Clemente Estable
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, N Y, USA
    J Biomed Sci 9:234-45. 2002
    Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T(1)...
  27. ncbi Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences
    Cyril F Bourgeois
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, England
    Mol Cell Biol 22:1079-93. 2002
    ..transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1...
  28. ncbi Nuclear receptor coactivator p160 proteins enhance the HIV-1 long terminal repeat promoter by bridging promoter-bound factors and the Tat-P-TEFb complex
    Tomoshige Kino
    Pediatric and Reproductive Endocrinology Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:2396-405. 2002
    ..the N-terminal region of Tat and to the host cell protein cyclin T1, respectively, which is normally complexed with CDK9 as P-TEFb...
  29. ncbi Inhibition of human immunodeficiency virus type 1 replication by RNA interference directed against human transcription elongation factor P-TEFb (CDK9/CyclinT1)
    Ya Lin Chiu
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Virol 78:2517-29. 2004
    The human positive transcription elongation factor P-TEFb is composed of two subunits, cyclin T1 (hCycT1) and CDK9, and is involved in transcriptional regulation of cellular genes as well as human immunodeficiency virus type 1 (HIV-1) ..
  30. ncbi TFIIH inhibits CDK9 phosphorylation during human immunodeficiency virus type 1 transcription
    M Zhou
    Virus Tumor Biology Section, Basic Research Laboratory, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:44633-40. 2001
    ..HIV-1), transcription elongation by recruitment of the human transcription elongation factor P-TEFb, consisting of CDK9 and cyclin T1, to the TAR RNA structure...
  31. ncbi Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome
    R E Kiernan
    Laboratoire de Virologie Moléculaire et Transfert de Gène, Institut de Genetique Humaine, UPR1142, Montpellier, France
    Mol Cell Biol 21:7956-70. 2001
    b>CDK9 paired with cyclin T1 forms the human P-TEFb complex and stimulates productive transcription through phosphorylation of the RNA polymerase II C-terminal domain...
  32. ncbi Transient induction of cyclin T1 during human macrophage differentiation regulates human immunodeficiency virus type 1 Tat transactivation function
    Li Ying Liou
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 76:10579-87. 2002
    ..of the integrated provirus by recruiting the kinase complex TAK/P-TEFb, composed of cyclin T1 (CycT1) and Cdk9, to the viral TAR RNA element...
  33. ncbi c-Myc recruits P-TEFb for transcription, cellular proliferation and apoptosis
    Satoshi Kanazawa
    Departments of Medicine, Microbiology and Immunology, Rosalind Russell Medical Research Center, University of California, San Francisco, CA 94143 0703, USA
    Oncogene 22:5707-11. 2003
    ..The cyclin-dependent kinase 9 (Cdk9) in P-TEFb then phosporylates the C-terminal domain of RNA polymerase II, which is required for the transition from ..
  34. ncbi A human immunodeficiency virus type 1 Tat-like arginine-rich RNA-binding domain is essential for HEXIM1 to inhibit RNA polymerase II transcription through 7SK snRNA-mediated inactivation of P-TEFb
    Jasper H N Yik
    Department of Molecular and Cellular Biology, University of California, Berkeley, 94720, USA
    Mol Cell Biol 24:5094-105. 2004
    The HEXIM1 protein inhibits the kinase activity of P-TEFb (CDK9/cyclin T) to suppress RNA polymerase II transcriptional elongation in a process that specifically requires the 7SK snRNA, which mediates the interaction of HEXIM1 with P-TEFb...
  35. ncbi Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter
    Céline Treand
    Institut Cochin, Département de Génétique et Développement, Paris, France
    EMBO J 25:1690-9. 2006
    ..These data show the requirement of Tat-mediated recruitment of SWI/SNF chromatin-remodeling complex to HIV-1 promoter in the activation of the LTR...
  36. ncbi HIV-1 tat protein and cell proliferation and survival: a brief review
    Davide Gibellini
    Department of Clinical and Experimental Medicine, Microbiology Section, University of Bologna, Italy
    New Microbiol 28:95-109. 2005
    ..This review focuses on some aspects of Tat biological activity with particular regard to effects of Tat on cell proliferation and survival regulation...
  37. ncbi Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186
    Qintong Li
    Department of Biochemistry and Molecular Biology Program, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 280:28819-26. 2005
    ..HEXIM.P-TEFb complex. Analysis of T-loop phosphorylation in Cdk9 indicated that phosphorylation of Thr(186), but not Ser(175), was essential for kinase activity and for recruitment ..
  38. ncbi Modulation of a P-TEFb functional equilibrium for the global control of cell growth and differentiation
    Nanhai He
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Mol Cell Biol 26:7068-76. 2006
    ..Concurrently, the P-TEFb equilibrium was shifted overwhelmingly toward the 7SK snRNP side. Together, these data link the P-TEFb equilibrium to the intracellular transcriptional demand and proliferative/differentiated states of cells...
  39. ncbi The carboxyl-terminal domain of RNA polymerase II is phosphorylated by a complex containing cdk9 and infected-cell protein 22 of herpes simplex virus 1
    Lizette O Durand
    The Marjorie B Kovler Viral Oncology Laboratories, The University of Chicago, 910 East 58th Street, Chicago, IL 60637, USA
    J Virol 79:6757-62. 2005
    ..Here we report another function of ICP22. Thus, ICP22 physically interacts with cdk9, a constitutively active cyclin-dependent kinase involved in transcriptional regulation...
  40. ncbi A new paradigm in eukaryotic biology: HIV Tat and the control of transcriptional elongation
    Matjaz Barboric
    Department of Medicine, Rosalind Russell Medical Research Center, University of California, San Francisco, USA
    PLoS Biol 3:e76. 2005
  41. ncbi HEXIM2, a HEXIM1-related protein, regulates positive transcription elongation factor b through association with 7SK
    Sarah A Byers
    Molecular Biology Program and Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 280:16360-7. 2005
    ..Our results provide strong evidence that HEXIM2 is a regulator of P-TEFb function. Furthermore, our results support the idea that the utilization of HEXIM1 or HEXIM2 to bind and inhibit P-TEFb can be differentially regulated in vivo...
  42. ncbi Compensatory contributions of HEXIM1 and HEXIM2 in maintaining the balance of active and inactive positive transcription elongation factor b complexes for control of transcription
    Jasper H N Yik
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    J Biol Chem 280:16368-76. 2005
    ..Our results demonstrate that there is a tightly regulated cellular process to maintain the balance between active and inactive P-TEFb complexes, which controls global transcription as well as cell growth and differentiation...
  43. ncbi HIV-1 TAR RNA: the target of molecular interactions between the virus and its host
    Sylvie Bannwarth
    McGill University AIDS Centre, Molecular Oncology Group, Lady Davis Institute for Medical Research, Canada
    Curr HIV Res 3:61-71. 2005
    ..The Tat-Cyclin T1-CDK9 complex is the main component in the trans-activation of HIV-1 and its affinity for TAR is regulated through Tat ..
  44. ncbi Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription
    Meisheng Zhou
    Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine, Washington, DC 20037, USA
    J Virol 78:13522-33. 2004
    ..Here we examine the role of the Tat/TAR-specified cyclin-dependent kinase 9 (CDK9) kinase activity in regulation of HIV-1 transcription elongation and histone methylation...
  45. ncbi A review of HIV-1 Tat protein biological effects
    A Pugliese
    Department of Medical and Surgical Sciences, Section of Clinical Microbiology of Turin University, Amedeo di Savoia Hospital, Italy
    Cell Biochem Funct 23:223-7. 2005
    ..The effective role of HIV-1 Tat protein in promoting viral replication and its high immunogenicity suggest useful employment of this protein for therapeutic or preventive vaccine preparations...
  46. ncbi Role of cdk9 in the optimization of expression of the genes regulated by ICP22 of herpes simplex virus 1
    Lizette Olga Durand
    Marjorie B Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, IL 60637, USA
    J Virol 82:10591-9. 2008
    ..ICP22 binds the cyclin-dependent kinase 9 (cdk9) but not cdk7, and this complex in conjunction with viral protein kinases phosphorylates the carboxyl terminus of ..
  47. ncbi Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor
    Annemieke A Michels
    UMR 8541 CNRS, Ecole Normale Superieure, Régulation de l Expression Génétique, Paris, France
    EMBO J 23:2608-19. 2004
    ..Core active P-TEFb is composed of CDK9 and cyclin T...
  48. ncbi The functional role of an interleukin 6-inducible CDK9.STAT3 complex in human gamma-fibrinogen gene expression
    Tieying Hou
    Department of Biochemistry, and Sealy Center for Molecular Medicine, University of Texas Medical Branch, Galveston, Texas 77555 1060, USA
    J Biol Chem 282:37091-102. 2007
    ..of the APR, we investigated the requirement of an IL-6-inducible complex of STAT3 with cyclin-dependent kinase 9 (CDK9) on gamma-FBG expression in HepG2 hepatocarcinoma cells...
  49. ncbi Multiple effects of HIV-1 trans-activator protein on the pathogenesis of HIV-1 infection
    M C D G Huigen
    Eijkman Winkler Center for Microbiology, Infectious Disease and Inflammation, Utrecht, The Netherlands
    Eur J Clin Invest 34:57-66. 2004
    ..These capacities together accelerate the progression towards AIDS and make Tat an interesting candidate as a constituent of an anti-AIDS vaccine...
  50. ncbi Dynamics of human immunodeficiency virus transcription: P-TEFb phosphorylates RD and dissociates negative effectors from the transactivation response element
    Koh Fujinaga
    Department of Medicine, Rosalind Russell Medical Research Center, University of California at San Francisco, 3rd and Parnassus Avenue, San Francisco, CA 94143 0703, USA
    Mol Cell Biol 24:787-95. 2004
    ..b>Cdk9 in P-TEFb also phosphorylates RD at sites next to its RNA recognition motif...
  51. ncbi C/EBPbeta regulates human immunodeficiency virus 1 gene expression through its association with cdk9
    Giuseppe Mameli
    Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, 1900 N 12th Street 015 96, Philadelphia, PA 19122, USA
    J Gen Virol 88:631-40. 2007
    ..The HIV-1 Tat protein recruits the human positive transcription elongation factor P-TEFb, consisting of cdk9 and cyclin T1, to the HIV-1 transactivation response (TAR) region...
  52. ncbi NF-kappaB binds P-TEFb to stimulate transcriptional elongation by RNA polymerase II
    M Barboric
    Howard Hughes Medical Institute Departments of Medicine, Microbiology, and Immunology University of California at San Francisco 94143, USA
    Mol Cell 8:327-37. 2001
    ..Remarkably, DRB inhibition sensitized cells to TNF-alpha-induced apoptosis. Thus, NF-kappaB requires P-TEFb to stimulate the elongation of transcription and P-TEFb plays an unexpected role in regulating apoptosis...
  53. ncbi Direct evidence that HIV-1 Tat stimulates RNA polymerase II carboxyl-terminal domain hyperphosphorylation during transcriptional elongation
    C Isel
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Mol Biol 290:929-41. 1999
    ..inhibited by low concentrations of 5,6-dichloro-1-beta- D -ribofuranosyl benzimidazole (DRB), a potent inhibitor of CDK9, the protein kinase subunit of the Tat-associated kinase (TAK)...
  54. ncbi Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1
    M Monsalve
    Dana Farber Cancer Institute and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:307-16. 2000
    ..PGC-1 can alter the processing of an mRNA, but only when it is loaded onto the promoter of the gene. These data demonstrate the coordinated regulation of RNA transcription and processing through PGC-1...
  55. ncbi Role of the human and murine cyclin T proteins in regulating HIV-1 tat-activation
    Y T Kwak
    Division of Hematology Oncology, Department of Medicine, Harold Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, 75235 8594, USA
    J Mol Biol 288:57-69. 1999
    ..The data indicate that a specific residue in the cyclin T proteins is required for their in vitro interaction with Tat and their ability to stimulate in vivo tat-activation...
  56. ncbi HIV-1 TAR RNA enhances the interaction between Tat and cyclin T1
    J Zhang
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 275:34314-9. 2000
    ..We conclude that TAR RNA nucleates the formation of the Tat.P-TEFb complex through an induced fit mechanism...
  57. ncbi Relief of two built-In autoinhibitory mechanisms in P-TEFb is required for assembly of a multicomponent transcription elongation complex at the human immunodeficiency virus type 1 promoter
    Y W Fong
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3206, USA
    Mol Cell Biol 20:5897-907. 2000
    ..We show here that the P-TEFb heterodimer of Cdk9-cyclin T1 is intrinsically incapable of forming a stable complex with Tat and TAR due to two built-in ..
  58. ncbi Human and rodent transcription elongation factor P-TEFb: interactions with human immunodeficiency virus type 1 tat and carboxy-terminal domain substrate
    Y Ramanathan
    Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Virol 73:5448-58. 1999
    ..transcriptional regulator Tat increases the efficiency of elongation, and complexes containing the cellular kinase CDK9 have been implicated in this process...
  59. ncbi Host-cell positive transcription elongation factor b kinase activity is essential and limiting for HIV type 1 replication
    O Flores
    Department of Biology, Tularik, Inc, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 96:7208-13. 1999
    ..Selective inhibition of the P-TEFb kinase may therefore provide a novel approach for developing chemotherapeutic agents against HIV-1...
  60. ncbi The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein
    M E Garber
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Genes Dev 12:3512-27. 1998
    ..at the carboxy-terminal edge of the cyclin domain, and we show that hCycT1 can interact simultaneously with Tat and CDK9 on TAR RNA in vitro...
  61. ncbi Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat
    P D Bieniasz
    Howard Hughes Medical Institute and Department of Genetics, Box 3025, Room 426, CARL Building, Duke University Medical Center, Research Drive, Durham, NC 27710, USA
    EMBO J 17:7056-65. 1998
    ....
  62. ncbi HIV-1 Tat interacts with cyclin T1 to direct the P-TEFb CTD kinase complex to TAR RNA
    M E Garber
    Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037-1099, USA
    Cold Spring Harb Symp Quant Biol 63:371-80. 1998
  63. ncbi Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription
    M Zhou
    Virus Tumor Biology Section, LRBGE, Division of Basic Sciences, National Cancer Institute, Bethesda, Maryland 20892, USA
    Mol Cell Biol 20:5077-86. 2000
    ..Our results demonstrate that cyclin-dependent kinase 7 (CDK7) (TFIIH) and CDK9 (P-TEFb) both associate with the HIV-1 preinitiation complex...
  64. ncbi Tat activates human immunodeficiency virus type 1 transcriptional elongation independent of TFIIH kinase
    D Chen
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Mol Cell Biol 19:2863-71. 1999
    ..transcriptional elongation by recruitment of the human transcription elongation factor P-TEFb, consisting of Cdk9 and cyclin T1, to the HIV-1 promoter via cooperative binding to the nascent HIV-1 transactivation response RNA ..
  65. ncbi Interaction of Sp1 transcription factor with HIV-1 Tat protein: looking for cellular partners
    Arianna Loregian
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35121, Padua, Italy
    FEBS Lett 543:61-5. 2003
    ..proteins recently shown to play a relevant role in Tat function, namely TATA box-binding protein, cyclin T1, CDK9, and cyclin T1/CDK9 complex, were coexpressed, individually or in pair-wise combination, with Tat and Sp1 hybrids...
  66. ncbi HIV-1 tat transcriptional activity is regulated by acetylation
    R E Kiernan
    Laboratoire de Virologie Moléculaire et Transfert de Gène, Institut de Genetique Humaine, UPR1142 Montpellier, 34396, France
    EMBO J 18:6106-18. 1999
    ..Mechanistically, acetylation at Lys28 by PCAF enhanced Tat binding to the Tat-associated kinase, CDK9/P-TEFb, while acetylation by p300 at Lys50 of Tat promoted the dissociation of Tat from TAR RNA that occurs during ..
  67. ncbi Recruitment of cyclin T1/P-TEFb to an HIV type 1 long terminal repeat promoter proximal RNA target is both necessary and sufficient for full activation of transcription
    P D Bieniasz
    Howard Hughes Medical Institute and Department of Genetics, Box 3025, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 96:7791-6. 1999
    ..Activation by artificially recruited CycT1 depended on the ability to bind the CDK9 component of P-TEFb...
  68. ncbi Transcription elongation factor P-TEFb mediates Tat activation of HIV-1 transcription at multiple stages
    Q Zhou
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    EMBO J 17:3681-91. 1998
    ..Recently, a human transcription elongation factor P-TEFb, consisting of CDK9 kinase, cyclin T and other associated factors, has been shown to interact with Tat to restore Tat activation in ..
  69. ncbi Cyclin box structure of the P-TEFb subunit cyclin T1 derived from a fusion complex with EIAV tat
    Kanchan Anand
    EMBL Heidelberg, Structural and Computational Biology and Developmental Biology Unit, 69117 Heidelberg, Germany
    J Mol Biol 370:826-36. 2007
    ..Tat) protein and the transactivation response (TAR) RNA element thereby activating cyclin dependent kinase 9 (Cdk9), which stimulates transcription at the level of chain elongation...
  70. ncbi Inhibition of HIV-1 replication by P-TEFb inhibitors DRB, seliciclib and flavopiridol correlates with release of free P-TEFb from the large, inactive form of the complex
    Sebastian Biglione
    Interdisciplinary Molecular and Cellular Biology Program, University of Iowa, Iowa City, IA, USA
    Retrovirology 4:47. 2007
    The positive transcription elongation factor, P-TEFb, comprised of cyclin dependent kinase 9 (Cdk9) and cyclin T1, T2 or K regulates the productive elongation phase of RNA polymerase II (Pol II) dependent transcription of cellular and ..
  71. ncbi Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription
    Tatyana Ammosova
    Center for Sickle Cell Disease, Howard University, 2121 Georgia Ave, N W Washington, DC 20059, USA
    Retrovirology 2:47. 2005
    HIV-1 Tat protein recruits human positive transcription elongation factor P-TEFb, consisting of CDK9 and cyclin T1, to HIV-1 transactivation response (TAR) RNA...
  72. ncbi T-loop phosphorylated Cdk9 localizes to nuclear speckle domains which may serve as sites of active P-TEFb function and exchange between the Brd4 and 7SK/HEXIM1 regulatory complexes
    Eugene C Dow
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    J Cell Physiol 224:84-93. 2010
    ..Core P-TEFb is comprised of Cdk9 and a cyclin regulatory subunit, with Cyclin T1 being the predominant Cdk9-associated cyclin...
  73. ncbi HEXIM1 forms a transcriptionally abortive complex with glucocorticoid receptor without involving 7SK RNA and positive transcription elongation factor b
    Noriaki Shimizu
    Division of Clinical Immunology and Department of Rheumatology and Allergy, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 102:8555-60. 2005
    ..RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent ..
  74. ncbi Transcription factor IIS cooperates with the E3 ligase UBR5 to ubiquitinate the CDK9 subunit of the positive transcription elongation factor B
    Marilena Cojocaru
    Institut de Recherches Cliniques de Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 286:5012-22. 2011
    ..We show that the E3 ubiquitin ligase UBR5 associates with the CDK9 subunit of positive transcription elongation factor b to mediate its polyubiquitination in human cells...
  75. ncbi Berberine regulates peroxisome proliferator-activated receptors and positive transcription elongation factor b expression in diabetic adipocytes
    Jiyin Zhou
    Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, PR China
    Eur J Pharmacol 649:390-7. 2010
    ..receptors (PPARs) and positive transcription elongation factor b (P-TEFb) (including cyclin-dependent kinase 9 (CDK9) and cyclin T1) in white adipose tissue of diabetic rats and RNA interference-treated 3T3-L1 cells...
  76. ncbi Characterization of the Schizosaccharomyces pombe Cdk9/Pch1 protein kinase: Spt5 phosphorylation, autophosphorylation, and mutational analysis
    Yi Pei
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    J Biol Chem 278:43346-56. 2003
    Schizosaccharomyces pombe Cdk9/Pch1 protein kinase is a functional ortholog of the essential Saccharomyces cerevisiae Bur1/Bur2 kinase and a putative ortholog of metazoan P-TEFb (Cdk9/cyclin T)...
  77. ncbi G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb)
    Tianyang Qi
    Key Laboratory of Molecular Epigenetics of MOE and the Institute of Genetics and Cytology, Northeast Normal University, Changchun, Jilin 130024, China
    J Biol Chem 286:15171-81. 2011
    ..Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation...
  78. ncbi Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo
    Yi Pei
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY
    Mol Cell Biol 26:777-88. 2006
    Cyclin-dependent kinase 9 (Cdk9) of fission yeast is an essential ortholog of metazoan positive transcription elongation factor b (P-TEFb), which is proposed to coordinate capping and elongation of RNA polymerase II (Pol II) transcripts...
  79. ncbi Peroxisome proliferator-activated receptor gamma recruits the positive transcription elongation factor b complex to activate transcription and promote adipogenesis
    Irena Iankova
    Institut National de la Sante et de la Recherche Medicale, Equipe Avenir, Unité 540, 60 rue de Navacelles, Montpellier, France
    Mol Endocrinol 20:1494-505. 2006
    ..The P-TEFb complex is composed of a cyclin-dependent kinase (cdk9) subunit and a regulatory partner (cyclin T1, cyclin T2, or cyclin K)...
  80. ncbi CDK9 a potential target for drug development
    Fernanda Canduri
    Faculdade de Biociências PUCRS Av Ipiranga, 6681 Porto Alegre RS Brazil
    Med Chem 4:210-8. 2008
    ..b>CDK9 is the catalytic subunit of positive transcription elongation factor b (P-TEFb)...
  81. ncbi Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy
    Jorge Espinoza-Derout
    Center for Cardiovascular and Muscle Research and Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York, 11203, USA
    Cardiovasc Res 75:129-38. 2007
    ....
  82. ncbi CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1
    Bartlomiej Bartkowiak
    Department of Biochemistry, Duke Center for RNA Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genes Dev 24:2303-16. 2010
    ..efficiently by a hCDK9 chimera than by a hCDK13 chimera, suggesting the following orthology relationships: Bur1 ↔ CDK9 and Ctk1 ↔ CDK12/13...
  83. ncbi Halogen bonds form the basis for selective P-TEFb inhibition by DRB
    Sonja Baumli
    Department of Biochemistry Laboratory of Molecular Biophysics, University of Oxford, UK
    Chem Biol 17:931-6. 2010
    b>Cdk9, the kinase of the positive transcription elongation factor b, is required for processive transcription elongation by RNA polymerase II...
  84. ncbi IMP dehydrogenase is recruited to the transcription complex through serine 2 phosphorylation of RNA polymerase II
    Jae Hyun Park
    Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University, 300 Cheoncheon dong, Jangan gu, Suwon, Gyeonggi do 440 746, Republic of Korea
    Biochem Biophys Res Commun 392:588-92. 2010
    ..A synthetic lethal screen using a deletion strain of Ctk1 kinase, a yeast homolog of mammalian Cdk9/P-TEFb that phosphorylates serine 2 within the RNA polymerase II (RNApII) C-terminal domain (CTD), identified that ..
  85. ncbi The CDK-activating kinase (CAK) Csk1 is required for normal levels of homologous recombination and resistance to DNA damage in fission yeast
    Hilary B Gerber
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    PLoS ONE 3:e1492. 2008
    ..pombe--Cdk1, Mcs6 and Cdk9, the catalytic subunit of positive transcription elongation factor b (P-TEFb)--but is not itself essential.
  86. ncbi Roles of Arabidopsis cyclin-dependent kinase C complexes in cauliflower mosaic virus infection, plant growth, and development
    Xiaofeng Cui
    Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Plant Cell 19:1388-402. 2007
    ..II is phosphorylated during the transcription cycle by three cyclin-dependent kinases (CDKs): CDK7, CDK8, and CDK9. CDK9 and its interacting cyclin T partners belong to the positive transcription elongation factor b (P-TEFb) ..
  87. ncbi A cyclin-dependent protein kinase, CDKC2, colocalizes with and modulates the distribution of spliceosomal components in Arabidopsis
    Georgios Kitsios
    John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 54:220-35. 2008
    ..of RNA polymerase II (RNAP II), and therefore the CDKC-cyclinT (CycT) complex may have a role similar to the animal CDK9-CycT complex of the positive transcription elongation factor b (P-TEFb)...
  88. ncbi [In vitro specific binding of Shiga toxin 1 and 2 by TAK-751S (Gb3 analog)]
    T Takeda
    Takeda Chemical Industries, Ltd, Pharmacology Laboratories, Pharmaceutical Research Division
    Kansenshogaku Zasshi 72:924-34. 1998
    b>TAK-751S is a synthetic trisaccharide coupled to Chromosorb P using a spacer sequence of 8-methoxycarboyloctyl (MCO)...
  89. ncbi Catalytic activity of Cdk9 is required for nuclear co-localization of the Cdk9/cyclin T1 (P-TEFb) complex
    Giuliana Napolitano
    Department of Genetics, Molecular and General Biology, University of Naples, Federico II, Naples, Italy
    J Cell Physiol 197:1-7. 2003
    b>Cdk9 and its binding partner cyclin T1 comprise the positive elongation factor b (P-TEFb). P-TEFb phosphorylates the RNA polymerase II carboxyl-terminal-domain (CTD) allowing efficient transcription elongation...
  90. ncbi A role for CDK9-cyclin K in maintaining genome integrity
    David S Yu
    Department of Radiation Oncology, Emory University School of Medicine, Atlanta, GA, USA
    Cell Cycle 10:28-32. 2011
    Cyclin-dependent kinase 9 (CDK9), with its cyclin T regulatory subunit, is a component of the positive transcription elongation factor b (P-TEFb) complex, which stimulates transcription elongation and also functions in co-transcriptional ..
  91. ncbi Role of the cyclin-dependent kinase 9-related pathway in mammalian gene expression and human diseases
    Gaetano Romano
    Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
    Cell Cycle 7:3664-8. 2008
    Cyclin-dependent kinase 9 (Cdk9) is a cdc2-like serine/threonine kinase. The so-called Cdk9-related pathway comprises two Cdk9 isoforms (Cdk9-42 and Cdk9-55), cyclin T1, cyclin T2a, cyclin T2b and cyclin K...
  92. ncbi Inhibiting lentiviral replication by HEXIM1, a cellular negative regulator of the CDK9/cyclin T complex
    Saki Shimizu
    AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan
    AIDS 21:575-82. 2007
    ..and depends on positive transcription elongation factor b complex (P-TEFb), composed of cyclin dependent kinase 9 (CDK9) and cyclin T...
  93. ncbi Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment
    Joel C Eissenberg
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd, St Louis, MO 63104, USA
    Mol Genet Genomics 277:101-14. 2007
    ..We knocked down the catalytic subunit of P-TEFb, Cdk9, in Drosophila melanogaster using RNA interference. Cdk9 knockdown flies die during metamorphosis...
  94. ncbi P-TEFb kinase recruitment and function at heat shock loci
    J T Lis
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 14:792-803. 2000
    P-TEFb, a heterodimer of the kinase Cdk9 and cyclin T, was isolated as a factor that stimulates formation of productive transcription elongation complexes in vitro...
  95. ncbi 55K isoform of CDK9 associates with Ku70 and is involved in DNA repair
    Hongbing Liu
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    Biochem Biophys Res Commun 397:245-50. 2010
    ..P-TEFb is a set of different molecular complexes, each containing CDK9 as the catalytic subunit...
  96. ncbi Recruitment of P-TEFb (Cdk9-Pch1) to chromatin by the cap-methyl transferase Pcm1 in fission yeast
    Allan Guiguen
    Laboratoire de Genetique Moleculaire GEMO, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
    EMBO J 26:1552-9. 2007
    ..We report that the fission yeast P-TEFb (Cdk9-Pch1) forms a complex with the cap-methyltransferase Pcm1 and these proteins colocalise on chromatin...
  97. ncbi Cyclin-dependent kinase 9-cyclin K functions in the replication stress response
    David S Yu
    Department of Radiation Oncology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    EMBO Rep 11:876-82. 2010
    Cyclin-dependent kinase 9 (CDK9) is a well-characterized subunit of the positive transcription elongation factor b complex in which it regulates transcription elongation in cooperation with cyclin T...
  98. ncbi Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control
    Yi Pei
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    J Biol Chem 278:7180-8. 2003
    ..elongation delay induced by negative factors Spt5/Spt4 and NELF, which is overcome by the positive factor P-TEFb (Cdk9/cyclin T), a protein kinase that phosphorylates the pol II C-terminal domain (CTD) and the transcription elongation ..
  99. ncbi RNA-driven cyclin-dependent kinase regulation: when CDK9/cyclin T subunits of P-TEFb meet their ribonucleoprotein partners
    Annemieke A Michels
    Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland
    Biotechnol J 3:1022-32. 2008
    The positive transcription elongation factor (P-TEFb) consists of CDK9, a cyclin-dependent kinase and its cyclin T partner. It is required for transcription of most class II genes. Its activity is regulated by non-coding RNAs...
  100. ncbi Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription
    Qi Wang
    University of Medicine and Dentistry of New Jersey, Department of Biochemistry and Molecular Biology, New Jersey Medical School, Newark, NJ 07103 2714, USA
    J Mol Biol 367:630-46. 2007
    Positive transcription elongation factor b (P-TEFb) complexes, composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1 or T2, are engaged by many cellular transcription regulators that activate or inhibit transcription from specific ..
  101. ncbi TFIIH and P-TEFb coordinate transcription with capping enzyme recruitment at specific genes in fission yeast
    Laia Viladevall
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 33:738-51. 2009
    ..To define their functions, we mutated the TFIIH-associated kinase Mcs6 and P-TEFb homologs Cdk9 and Lsk1 of fission yeast, making them sensitive to inhibition by bulky purine analogs...

Research Grants63

  1. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2007
    The human transcription elongation factor P-TEFb, consisting of a CDK9/cyclin T heterodimer, promotes both general and HIV-specific elongation by phosphorylating RNA polymerase II and overcoming pausing by negative elongation factors...
  2. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2009
    The general transcription factor P-TEFb, consisting of Cdk9 and cyclin T, strongly stimulates RNA polymerase II elongation. It is also a host cell cofactor for Tat activation of HIV-1 transcription...
  3. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2010
    The general transcription factor P-TEFb, consisting of Cdk9 and cyclin T, strongly stimulates RNA polymerase II elongation. It is also a host cell cofactor for Tat activation of HIV-1 transcription...
  4. Regulation of Transcriptional Elongation by HIV-1 TAT
    Qiang Zhou; Fiscal Year: 2009
    The general transcription factor P-TEFb, consisting of Cdk9 and cyclin T, strongly stimulates RNA polymerase II elongation. It is also a host cell cofactor for Tat activation of HIV-1 transcription...
  5. Tat Transactivation
    BORIS MATIJA PETERLIN; Fiscal Year: 2010
    ..P-TEFb contains the Cyclin dependent kinase 9 (Cdk9) and a C-type cyclin, CycT1, CycT2 or CycK. NF-KB contains p65 (RelA) and p50...
  6. Tat Transactivation
    B Matija Peterlin; Fiscal Year: 2007
    ..P-TEFb contains the Cyclin dependent kinase 9 (Cdk9) and a C-type cyclin, CycT1, CycT2 or CycK. NF-KB contains p65 (RelA) and p50...
  7. Tat Transactivation
    BORIS PETERLIN; Fiscal Year: 2004
    ..Together, they bind with high affinity and specificity to the transactivation response (TAR) RNA structure. Cdk9 in P-TEFb then phosphorylates the C-terminal domain (CTD) or RNA polymerase II (RNAPII)...
  8. CYCLIN T AND HIV1 TAT TRANSACTIVATION
    Katherine Jones; Fiscal Year: 2007
    ..Tat interacts directly with Cyclin T1, the regulatory subunit of CDK9 in the cellular P-TEFb (positive transcription elongation factor) complex...
  9. Structure and Function of Ribonucleoprotein Complexes Modulating HIV Replication
    Tariq M Rana; Fiscal Year: 2010
    ..Tat interacts with cyclinT1 (CycT1), a regulatory partner of CDK9 in the positive transcription elongation factor b (P-TEFb) complex, and binds cooperatively with CycT1 to TAR RNA...
  10. IMPACT OF TUBERCULOSIS ON HIV DISEASE
    ZAHRA TOOSSI TOOSSI; Fiscal Year: 2010
    ..These include;1. Derivatives of erythromycin, some of which may be useful in MDR TB, 2. an inhibitor of CDK9 (of P-TEFb), Indirubicin Monoxime, and 3...
  11. Cyclin-dependent Kinases and Cardiac Growth
    Michael Schneider; Fiscal Year: 2007
    ..basal transcription factors, TFIIH (cyclin HCdk7) and positive transcription elongation factor b (P-TEFb, cyclin T-Cdk9)...
  12. Regulation of HIV transcription by Tat and P-TEPb
    TSAFI PE ERY; Fiscal Year: 2004
    ..P-TEFb (cyclin T1/CDK9) is a cellular transcription elongation factor that cooperates with the viral protein Tal to attain processive ..
  13. REGULATION OF HIV1 GENE EXPRESSION BY TAT AND TAR
    Michael Mathews; Fiscal Year: 2001
    ..TAK and P-TEFb share at least one subunit, the CDC2-related kinase PITALRE. The major objectives of this grant are to evaluate the hypothesis that Tat acts via TAK/P-TEFb, and to extend it ..
  14. Tat Transactivation
    BORIS MATIJA PETERLIN; Fiscal Year: 2011
    ..Importantly, CycK does not bind Cdk9, which together with CycT1 or CycT2 forms P-TEFb...
  15. CYCLIN T AND HIV1 TAT TRANSACTIVATION
    Katherine Jones; Fiscal Year: 2003
    ..Cyclin T (CycT) is a predominant cyclin partner for CDK9 (PITALRE), the catalytic subunit of the positive-acting transcription elongation factor complex, P-TEFb...
  16. MRNA CAPPING ENZYME
    Stewart Shuman; Fiscal Year: 2006
    ..with other factors, elicits an elongation arrest that is overcome by the protein kinase activity of P-TEFb (Cdk9/cyclinT)...
  17. Structure and Function of HIV Tat-TAR Complex
    TARIQ RANA; Fiscal Year: 2006
    ..Tat interacts with cyclinTl (CycTl), a regulatory partner of CDK9 in the positive transcription elongation factor b (P-TEFb) complex, and binds cooperatively with CycT 1 to TAR RNA...