Gene Symbol: cdk7
Description: cyclin dependent kinase 7
Alias: CAK, CAK1, CDKN7, HCAK, MO15, STK1, p39MO15, cyclin-dependent kinase 7, 39 KDa protein kinase, CDK-activating kinase 1, TFIIH basal transcription factor complex kinase subunit, cell division protein kinase 7, cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase), homolog of Xenopus MO15 Cdk-activating kinase, kinase subunit of CAK, serine/threonine kinase stk1, serine/threonine protein kinase 1, serine/threonine protein kinase MO15
Species: human
Products:     cdk7

Top Publications

  1. Tassan J, Schultz S, Bartek J, Nigg E. Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase). J Cell Biol. 1994;127:467-78 pubmed
    ..It was termed CAK (for cdk-activating kinase), and it was shown to contain p40MO15 as its catalytic subunit...
  2. Nekhai S, Shukla R, Fernandez A, Kumar A, Lamb N. Cell cycle-dependent stimulation of the HIV-1 promoter by Tat-associated CAK activator. Virology. 2000;266:246-56 pubmed
    ..We reported a Tat-associated CAK/CTD kinase from mitogenically induced primary human T-cells (TTK) (S. Nekhai et al., 1997, J. Virol. 71, 7436-7441)...
  3. Larochelle S, Batliner J, Gamble M, Barboza N, Kraybill B, Blethrow J, et al. Dichotomous but stringent substrate selection by the dual-function Cdk7 complex revealed by chemical genetics. Nat Struct Mol Biol. 2006;13:55-62 pubmed
    b>Cdk7 performs two essential but distinct functions as a CDK-activating kinase (CAK) required for cell-cycle progression and as the RNA polymerase II (Pol II) CTD kinase of general transcription factor IIH...
  4. Glover Cutter K, Larochelle S, Erickson B, Zhang C, Shokat K, Fisher R, et al. TFIIH-associated Cdk7 kinase functions in phosphorylation of C-terminal domain Ser7 residues, promoter-proximal pausing, and termination by RNA polymerase II. Mol Cell Biol. 2009;29:5455-64 pubmed publisher
    The function of human TFIIH-associated Cdk7 in RNA polymerase II (Pol II) transcription and C-terminal domain (CTD) phosphorylation was investigated in analogue-sensitive Cdk7(as/as) mutant cells where the kinase can be inhibited without ..
  5. Darbon J, Devault A, Taviaux S, Fesquet D, Martinez A, Galas S, et al. Cloning, expression and subcellular localization of the human homolog of p40MO15 catalytic subunit of cdk-activating kinase. Oncogene. 1994;9:3127-38 pubmed
    ..for cdk-activating kinase, has been recently purified from starfish and Xenopus oocytes and shown to contain the MO15 gene product as a catalytic subunit...
  6. Fisher R, Morgan D. A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell. 1994;78:713-24 pubmed
    ..Protein sequencing indicates that the 42 kDa subunit is the mammalian homolog of MO15, a protein kinase known to be a component of CAK in amphibians and echinoderms...
  7. Rossignol M, Kolb Cheynel I, Egly J. Substrate specificity of the cdk-activating kinase (CAK) is altered upon association with TFIIH. EMBO J. 1997;16:1628-37 pubmed
    ..Three subunits of TFIIH, cdk7, cyclin H and MAT1, form a ternary complex, cdk-activating kinase (CAK), found either on its own or as part of ..
  8. Nekhai S, Shukla R, Kumar A. A human primary T-lymphocyte-derived human immunodeficiency virus type 1 Tat-associated kinase phosphorylates the C-terminal domain of RNA polymerase II and induces CAK activity. J Virol. 1997;71:7436-41 pubmed
    ..About 60% of Tat-associated CTD kinase copurifies with CDK2 kinase activity and contains the CAK components CDK7 and cyclin H...
  9. Cujec T, Okamoto H, Fujinaga K, Meyer J, Chamberlin H, Morgan D, et al. The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II. Genes Dev. 1997;11:2645-57 pubmed
    ..In this study, we demonstrate that Tat binds directly to the cyclin-dependent kinase 7 (CDK7), which leads to productive interactions between Tat and the CDK-activating kinase (CAK) complex and between Tat ..

More Information

Publications109 found, 100 shown here

  1. Ko L, Shieh S, Chen X, Jayaraman L, Tamai K, Taya Y, et al. p53 is phosphorylated by CDK7-cyclin H in a p36MAT1-dependent manner. Mol Cell Biol. 1997;17:7220-9 pubmed a cellular kinase required for the activation of a CDK-cyclin complex, and CAK is comprised of three subunits: CDK7, cyclin H, and p36MAT1...
  2. Kim J, Sharp P. Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase. J Biol Chem. 2001;276:12317-23 pubmed
    ..In addition, we provide several lines of evidence that P-TEFb is a CDK-activating kinase (CAK)-independent kinase...
  3. Ramanathan Y, Rajpara S, Reza S, Lees E, Shuman S, Mathews M, et al. Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences. J Biol Chem. 2001;276:10913-20 pubmed
    b>CDK7, CDK8, and CDK9 are cyclin-dependent kinases (CDKs) that phosphorylate the C-terminal domain (CTD) of RNA polymerase II. They have distinct functions in transcription...
  4. Pinhero R, Liaw P, Bertens K, Yankulov K. Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II. Eur J Biochem. 2004;271:1004-14 pubmed
    ..partially regulated by the phosphorylation of the CTD by three cyclin-dependent protein kinases (CDKs), namely CDK7, CDK8 and CDK9...
  5. Larochelle S, Merrick K, Terret M, Wohlbold L, Barboza N, Zhang C, et al. Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Mol Cell. 2007;25:839-50 pubmed
    ..The only known CDK-activating kinase (CAK) in metazoans is Cdk7, which is also part of the transcription machinery...
  6. Bockstaele L, Bisteau X, Paternot S, Roger P. Differential regulation of cyclin-dependent kinase 4 (CDK4) and CDK6, evidence that CDK4 might not be activated by CDK7, and design of a CDK6 activating mutation. Mol Cell Biol. 2009;29:4188-200 pubmed publisher T172 and T177 (respectively) by the only CDK-activating kinase identified in animal cells, cyclin H-CDK7. At odds with the existing data showing the constitutive activity of CDK7, we have recently identified the T172 ..
  7. St Amour C, Sansó M, Bösken C, Lee K, Larochelle S, Zhang C, et al. Separate domains of fission yeast Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition. Mol Cell Biol. 2012;32:2372-83 pubmed publisher
    ..The extension is dispensable, however, for recognition of CTD substrates "primed" by Mcs6 (Cdk7). On defined peptide substrates in vitro, Cdk9 prefers CTD repeats phosphorylated at Ser7 over unmodified repeats...
  8. Schachter M, Merrick K, Larochelle S, Hirschi A, Zhang C, Shokat K, et al. A Cdk7-Cdk4 T-loop phosphorylation cascade promotes G1 progression. Mol Cell. 2013;50:250-60 pubmed publisher
    ..Chemical genetics uncovered requirements for the metazoan CAK Cdk7 in determining cyclin specificity and activation order of Cdk2 and Cdk1 during S and G2 phases...
  9. García Martínez L, Mavankal G, Neveu J, Lane W, Ivanov D, Gaynor R. Purification of a Tat-associated kinase reveals a TFIIH complex that modulates HIV-1 transcription. EMBO J. 1997;16:2836-50 pubmed
    ..These results define a cellular kinase complex whose activity is modulated by Tat to result in activation of HIV-1 trancription. ..
  10. Isel C, Karn J. Direct evidence that HIV-1 Tat stimulates RNA polymerase II carboxyl-terminal domain hyperphosphorylation during transcriptional elongation. J Mol Biol. 1999;290:929-41 pubmed
    ..We conclude that activation of the CDK9 kinase, leading to CTD phosphorylation, occurs only in elongation complexes that have transcribed through the Tat-recognition element, TAR RNA. ..
  11. Garrett S, Barton W, Knights R, Jin P, Morgan D, Fisher R. Reciprocal activation by cyclin-dependent kinases 2 and 7 is directed by substrate specificity determinants outside the T loop. Mol Cell Biol. 2001;21:88-99 pubmed
    Cyclin-dependent kinase 7 (CDK7) is the catalytic subunit of the metazoan CDK-activating kinase (CAK), which activates CDKs, such as CDC2 and CDK2, through phosphorylation of a conserved threonine residue in the T loop...
  12. Nekhai S, Zhou M, Fernandez A, Lane W, Lamb N, Brady J, et al. HIV-1 Tat-associated RNA polymerase C-terminal domain kinase, CDK2, phosphorylates CDK7 and stimulates Tat-mediated transcription. Biochem J. 2002;364:649-57 pubmed
    ..CTD kinases associated with general transcription factors of the promoter complex, specifically TFIIH-associated CDK7 and positive transcription factor b-associated CDK9 (cyclin-dependent kinase 9)...
  13. Bockstaele L, Kooken H, Libert F, Paternot S, Dumont J, De Launoit Y, et al. Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors". Mol Cell Biol. 2006;26:5070-85 pubmed
    ..Collectively, these unexpected observations indicate that CDK4-activating kinase(s) should be reconsidered. ..
  14. Wang Y, Sun X, Fang L, Li K, Yang P, Du L, et al. Genomic instability in adult men involved in processing electronic waste in Northern China. Environ Int. 2018;117:69-81 pubmed publisher
    ..In males involved in handling of e-waste, there were 13 genes - ATM, ATR, ABL1, CHEK1, CHEK2, GADD45A, CDK7, GTSE1, OGG1, DDB1, PRKDC, XRCC1 and CCNH - for which expression of mRNA was up-regulated and 7 genes - BRCA1, ..
  15. Shi T, Sun J, Wu X, Weng J, Wang P, Qie H, et al. Transcriptome analysis of Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit treated with heat and 1-MCP. Plant Physiol Biochem. 2018;133:40-49 pubmed publisher
    ..These results might improve our understanding of the mechanisms of heat and 1-MCP inhibition of fruit postharvest physiology and prolongation of fruit shelf life. ..
  16. Saito Y, Moriya S, Kazama H, Hirasawa K, Miyahara K, Kokuba H, et al. Amino acid starvation culture condition sensitizes EGFR-expressing cancer cell lines to gefitinib-mediated cytotoxicity by inducing atypical necroptosis. Int J Oncol. 2018;52:1165-1177 pubmed publisher
    ..Therefore, the enhanced cytotoxic effect of GEF under AAS culture conditions is thought to be mediated by atypical necroptosis. ..
  17. Lu H, Yu D, Hansen A, Ganguly S, Liu R, Heckert A, et al. Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II. Nature. 2018;558:318-323 pubmed publisher
    ..domain-is trapped within the cyclin T1 droplets, and this process is enhanced upon pre-phosphorylation by CDK7 of transcription initiation factor TFIIH1-3...
  18. Gao L, Tang W, Ding Z, Wang D, Qi X, Wu H, et al. Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-?B/c-Myc Pathway in Pancreatic ? cells. Mol Med. 2015;21:154-66 pubmed publisher
    ..Therapeutic opportunities for type 2 diabetes may arise when its kinase catalytic function, but not its protein-binding function, is downregulated. ..
  19. Sadik H, Korangath P, Nguyen N, Gyorffy B, Kumar R, Hedayati M, et al. HOXC10 Expression Supports the Development of Chemotherapy Resistance by Fine Tuning DNA Repair in Breast Cancer Cells. Cancer Res. 2016;76:4443-56 pubmed publisher
    ..HOXC10 achieves integration of these functions by binding to, and activating cyclin-dependent kinase, CDK7, which regulates transcription by phosphorylating the carboxy-terminal domain of RNA polymerase II...
  20. Abaza M, Orabi K, Al Quattan E, Al Attiyah R. Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell Int. 2015;15:46 pubmed publisher
    ..Nar altered the expression of apoptosis and cell-cycle regulatory genes by down-regulating Cdk4, Cdk6, Cdk7, Bcl2, x-IAP and c-IAP-2 and up-regulating p18, p19, p21, caspases 3, 7, 8 and 9, Bak, AIF and Bax in both ..
  21. Basit F, van Oppen L, Schöckel L, Bossenbroek H, van Emst de Vries S, Hermeling J, et al. Mitochondrial complex I inhibition triggers a mitophagy-dependent ROS increase leading to necroptosis and ferroptosis in melanoma cells. Cell Death Dis. 2017;8:e2716 pubmed publisher
  22. Liu X, Zhang P, Ji K, Zhang J, Yang S, Du B, et al. Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice. Acta Histochem. 2018;120:15-21 pubmed publisher
    ..The results showed that MAP3K6 was inhibited by phosphorylated CDK5 through its activator CDK7. The decrease in MAP3K6 levels caused down-regulation of MEK1 and ERK expression, leading to the up-regulation of ..
  23. Ting P, Johnson C, Fang C, Cao X, Graeber T, Mattos C, et al. Tyrosine phosphorylation of RAS by ABL allosterically enhances effector binding. FASEB J. 2015;29:3750-61 pubmed publisher
    ..These data suggest that RAS phosphorylation at Tyr(137) allosterically alters protein conformation and effector binding, providing a mechanism for effector-initiated modulation of RAS signaling. ..
  24. Pak V, Eifler T, Jäger S, Krogan N, Fujinaga K, Peterlin B. CDK11 in TREX/THOC Regulates HIV mRNA 3' End Processing. Cell Host Microbe. 2015;18:560-70 pubmed publisher
    Transcriptional cyclin-dependent kinases play important roles in eukaryotic gene expression. CDK7, CDK9 (P-TEFb), and CDK13 are also critical for HIV replication. However, the function of CDK11 remained enigmatic...
  25. Srikumar T, Padmanabhan J. Potential Use of Flavopiridol in Treatment of Chronic Diseases. Adv Exp Med Biol. 2016;929:209-228 pubmed
    ..arrests cell cycle progression in the G1 or G2 phase by inhibiting the kinase activities of CDK1, CDK2, CDK4/6, and CDK7. Additionally, it binds tightly to CDK9, a component of the P-TEFb complex (CDK9/cyclin T), and interferes with RNA ..
  26. Cui H, Zhu Y, Jiang D. The RIP1-RIP3 Complex Mediates Osteocyte Necroptosis after Ovariectomy in Rats. PLoS ONE. 2016;11:e0150805 pubmed publisher
    ..These findings provide evidence of a potential mechanism through which osteocyte necroptosis is associated with postmenopausal osteoporosis. ..
  27. Reese J, Bruinsma E, Monroe D, Negron V, Suman V, Ingle J, et al. ER? inhibits cyclin dependent kinases 1 and 7 in triple negative breast cancer. Oncotarget. 2017;8:96506-96521 pubmed publisher
    ..siRNA mediated knockdown or drug inhibition of CDK1 and CDK7 in TNBC cells resulted in substantial decreases in proliferation regardless of ER? expression...
  28. Rocha A, Paternot S, Coulonval K, Dumont J, Soares P, Roger P. Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation. Mol Biol Cell. 2008;19:4814-25 pubmed publisher
  29. Harrod A, Fulton J, Nguyen V, Periyasamy M, Ramos Garcia L, Lai C, et al. Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer. Oncogene. 2017;36:2286-2296 pubmed publisher
    ..The CDK7 inhibitor, THZ1 prevented Ser118 phosphorylation and inhibited growth of MCF7-Y537S cells...
  30. Akoulitchev S, Reinberg D. The molecular mechanism of mitotic inhibition of TFIIH is mediated by phosphorylation of CDK7. Genes Dev. 1998;12:3541-50 pubmed
    ..A member of the cyclin-dependent kinase family, CDK7, is the kinase subunit of TFIIH...
  31. Schneider E, Kartarius S, Schuster N, Montenarh M. The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H. Oncogene. 2002;21:5031-7 pubmed
    ..Phosphorylation at this position had no influence on the assembly of the cyclin H/cdk7/Mat1 complex...
  32. Slattery M, Herrick J, Lundgreen A, Fitzpatrick F, Curtin K, Wolff R. Genetic variation in a metabolic signaling pathway and colon and rectal cancer risk: mTOR, PTEN, STK11, RPKAA1, PRKAG2, TSC1, TSC2, PI3K and Akt1. Carcinogenesis. 2010;31:1604-11 pubmed publisher
    ..These data suggest that genetic variation in a predefined candidate pathway for colorectal cancer contributes to both colon and rectal cancer risk. Associations appear to be strongest for CIMP+ and MSI+ tumors. ..
  33. Abdullah A, Zhang H, Nie Y, Tang W, Sun T. CDK7 and miR-210 Co-regulate Cell-Cycle Progression of Neural Progenitors in the Developing Neocortex. Stem Cell Reports. 2016;7:69-79 pubmed publisher
    ..Moreover, the cyclin-dependent kinase CDK7 is regulated by miR-210 and is necessary for normal NP cell-cycle progression...
  34. Oqani R, Lin T, Lee J, Kim S, Kang J, Jin D. Effects of CDK inhibitors on the maturation, transcription, and MPF activity of porcine oocytes. Reprod Biol. 2017;17:320-326 pubmed publisher
    ..The inhibitions of CDK2, CDK4, or CDK6 had no effect on cumulus expansion or GVBD. The catalytic activity of CDK7 was crucial for GVBD but less important for cumulus expansion, whereas inhibition of CDK9 severely blocked both ..
  35. Kobelt D, Karn T, Hock B, Holtrich U, Bräuninger A, Wolf G, et al. Human and Xenopus mo15 messenger-RNA are highly conserved but show different patterns of expression in adult tissues. Oncol Rep. 1994;1:1269-75 pubmed
    ..CAK (cdk activating kinase) containing p40(MO15) from Xenopus egg extracts phosphorylates and activates p34(cdc2) in a cyclin dependent manner at Thr 161...
  36. Tio C, Omerza G, Sunder S, Winter E. Autophosphorylation of the Smk1 MAPK is spatially and temporally regulated by Ssp2 during meiotic development in yeast. Mol Biol Cell. 2015;26:3546-55 pubmed publisher
    ..Instead, the T is phosphorylated by the cyclin-dependent kinase (CDK)-activating kinase, Cak1. The Y is autophosphorylated in an intramolecular reaction that requires a meiosis-specific protein named Ssp2...
  37. Patel H, Periyasamy M, Sava G, Bondke A, Slafer B, Kroll S, et al. ICEC0942, an Orally Bioavailable Selective Inhibitor of CDK7 for Cancer Treatment. Mol Cancer Ther. 2018;17:1156-1166 pubmed publisher
    ..b>CDK7 additionally regulates the activities of a number of transcription factors, including estrogen receptor (ER)-α...
  38. Serizawa H, Mäkelä T, Conaway J, Conaway R, Weinberg R, Young R. Association of Cdk-activating kinase subunits with transcription factor TFIIH. Nature. 1995;374:280-2 pubmed
    ..We report here that two polypeptides of the purified mammalian TFIIH are the MO15/Cdk7 kinase and cyclin H subunits of the Cdk-activating kinase Cak, previously identified as a positive regulator ..
  39. Liu J, Flanagan W, Drazba J, Estes M, Barnett G, Haqqi T, et al. The CDK inhibitor, p27Kip1, is required for IL-4 regulation of astrocyte proliferation. J Immunol. 1997;159:812-9 pubmed
    ..Reduced cyclin E-associated cdk2 activity was not due to impaired cyclin-dependent kinase-activating kinase (CAK) activity, which was unaffected by IL-4...
  40. Lee D, Duan H, Chang C. From androgen receptor to the general transcription factor TFIIH. Identification of cdk activating kinase (CAK) as an androgen receptor NH(2)-terminal associated coactivator. J Biol Chem. 2000;275:9308-13 pubmed
    ..Co-transfection of cdk activating kinase (CAK), the kinase moiety of TFIIH, enhanced AR-mediated transcription in a ligand-dependent manner in human prostate ..
  41. Lizcano J, Deak M, Morrice N, Kieloch A, Hastie C, Dong L, et al. Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo. J Biol Chem. 2002;277:27839-49 pubmed
  42. Liu H, Zhang S, Ma J, Dai Y, Li C, Lyu X, et al. Two Cdc2 Kinase Genes with Distinct Functions in Vegetative and Infectious Hyphae in Fusarium graminearum. PLoS Pathog. 2015;11:e1004913 pubmed publisher
    ..graminearum uniquely has two Aurora kinase genes and one additional putative cyclin gene, and its orthologs of CAK1 and other four essential mitotic kinases in the budding yeast are dispensable for viability...
  43. Kant S, Asthana S, Missiakas D, Pancholi V. A novel STK1-targeted small-molecule as an "antibiotic resistance breaker" against multidrug-resistant Staphylococcus aureus. Sci Rep. 2017;7:5067 pubmed publisher
    Ser/Thr protein kinase (STK1) plays a critical role in cell wall biosynthesis of and drug resistance in methicillin-resistant Staphylococcus aureus (MRSA)...
  44. Carugo A, Draetta G. Collapsing the Tumor Ecosystem: Preventing Adaptive Response to Treatment by Inhibiting Transcription. Cancer Discov. 2018;8:17-19 pubmed publisher
    ..Rusan and colleagues report that drug-tolerant cells are sensitive to THZ1, a dual CDK7/12 inhibitor, which, by impairing the transcriptional machinery, can prevent cellular rewiring to survive ..
  45. Hirschler Laszkiewicz I, Cavanaugh A, Mirza A, Lun M, Hu Q, Smink T, et al. Rrn3 becomes inactivated in the process of ribosomal DNA transcription. J Biol Chem. 2003;278:18953-9 pubmed
    ..Our results indicate that Rrn3 functions stoichiometrically in rDNA transcription and that its ability to associate with RNA polymerase I is lost upon transcription. ..
  46. Larochelle S, Amat R, Glover Cutter K, Sansó M, Zhang C, Allen J, et al. Cyclin-dependent kinase control of the initiation-to-elongation switch of RNA polymerase II. Nat Struct Mol Biol. 2012;19:1108-15 pubmed publisher
    ..Here we show that selective inhibition of Cdk7--part of TFIIH--increases TFIIE retention, prevents DRB sensitivity-inducing factor (DSIF) recruitment and ..
  47. Karpeta A, Maniecka A, Gregoraszczuk E. Different mechanisms of action of 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47) and its metabolites (5-OH-BDE-47 and 6-OH-BDE-47) on cell proliferation in OVCAR-3 ovarian cancer cells and MCF-7 breast cancer cells. J Appl Toxicol. 2016;36:1558-1567 pubmed publisher
    ..In OVCAR-3 cells, the parent compound stimulated cell proliferation by activating CDK1, CDK7, E2F1 and E2F2...
  48. Bischoff M, Brelle S, Minatelli S, Molle V. Stk1-mediated phosphorylation stimulates the DNA-binding properties of the Staphylococcus aureus SpoVG transcriptional factor. Biochem Biophys Res Commun. 2016;473:1223-1228 pubmed publisher
    ..Here we demonstrate that SpoVG can be phosphorylated by the staphylococcal Ser/Thr protein kinase Stk1 and that phosphorylation positively affects its DNA-binding properties...
  49. Luo S, Horvitz H. The CDK8 Complex and Proneural Proteins Together Drive Neurogenesis from a Mesodermal Lineage. Curr Biol. 2017;27:661-672 pubmed publisher
    ..Genetic analysis revealed that CDK-8 most likely promotes I4 neurogenesis by inhibiting the CDK-7/CYH-1 (CDK7/cyclin H) kinase module of the transcription initiation factor TFIIH...
  50. Woolford C, Lagree K, Aleynikov T, Mitchell A. Negative control of Candida albicans filamentation-associated gene expression by essential protein kinase gene KIN28. Curr Genet. 2017;63:1073-1079 pubmed publisher
    ..Reduced expression of the essential protein kinase gene CAK1 promoted filamentation-associated gene expression and biofilm formation in strains that lacked key transcriptional ..
  51. Fayaz S, Rajanikant G. Ensembling and filtering: an effective and rapid in silico multitarget drug-design strategy to identify RIPK1 and RIPK3 inhibitors. J Mol Model. 2015;21:314 pubmed publisher
    ..Graphical Abstract A generalized workflow employed in this study. Subsequent utilization of EPS and PEPSF might lead to reduced computational time and load. ..
  52. Deshmukh A, Mitra P, Maruthi M. Cdk7 mediates RPB1-driven mRNA synthesis in Toxoplasma gondii. Sci Rep. 2016;6:35288 pubmed publisher
    ..Role of Cdk7 is well characterized in model eukaryotes however its relevance in protozoan parasites has not been investigated...
  53. Liu J, Chen H, Wang D, Wang H, Deng Q. Pim-1 Kinase Regulating Dynamics Related Protein 1 Mediates Sevoflurane Postconditioning-induced Cardioprotection. Chin Med J (Engl). 2017;130:309-317 pubmed publisher
    ..Upregulation of Pim-1 might phosphorylate Drp1 and prevent extensive mitochondrial fission through Drp1 cytosolic sequestration. ..
  54. Gnatt A, Cramer P, Fu J, Bushnell D, Kornberg R. Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution. Science. 2001;292:1876-82 pubmed
    ..Protein-nucleic acid contacts help explain DNA and RNA strand separation, the specificity of RNA synthesis, "abortive cycling" during transcription initiation, and RNA and DNA translocation during transcription elongation. ..
  55. Graf L, Feichtinger S, Naing Z, Hutterer C, Milbradt J, Webel R, et al. New insight into the phosphorylation-regulated intranuclear localization of human cytomegalovirus pUL69 mediated by cyclin-dependent kinases (CDKs) and viral CDK orthologue pUL97. J Gen Virol. 2016;97:144-51 pubmed publisher
    ..CDK inhibitors, including a novel inhibitor of the CDK-activating kinase CDK7, massively intensified this fine-speckled accumulation...
  56. Jin X, Yu W, Nie Y, Liu M, Yan J. Synthesis and characterization of [(HPO3)6Mo21O60(H2O)4](8-): a new redox active heteropoly blue cluster with layered shape containing a phosphite template that self-assembles under controlled microwave irradiation. Dalton Trans. 2016;45:3268-71 pubmed publisher
    ..HPO3)6Mo21O60(H2O)4](8-), templated by six phosphite anions whereby two phosphites template a tri-lucunary {Mo15} Dawson structure, and one templates a {Mo6} ring with two of the three remaining phosphites acting as bridging ..
  57. Wang K, Liu F, Liu C, An T, Zhang J, Zhou L, et al. The long noncoding RNA NRF regulates programmed necrosis and myocardial injury during ischemia and reperfusion by targeting miR-873. Cell Death Differ. 2016;23:1394-405 pubmed publisher
    ..Our results identify a novel mechanism involving NRF and miR-873 in regulating programmed necrosis in the heart and suggest a potential therapeutic avenue for cardiovascular diseases. ..
  58. Szobi A, Rajtik T, ADAMEOVA A. Effects of necrostatin-1, an inhibitor of necroptosis, and its inactive analogue Nec-1i on basal cardiovascular function. Physiol Res. 2016;65:861-865 pubmed
    ..These effects of Nec-1 are likely due to its off-target action or RIP1 has an important role in the regulation of cardiovascular function independently of its action on the necroptotic pathway. ..
  59. Scott N, Giogha C, Pollock G, Kennedy C, Webb A, Williamson N, et al. The bacterial arginine glycosyltransferase effector NleB preferentially modifies Fas-associated death domain protein (FADD). J Biol Chem. 2017;292:17337-17350 pubmed publisher
    ..We conclude that, at normal levels of expression during bacterial infection, NleB1/NleBCR antagonizes death receptor-induced apoptosis of infected cells by modifying FADD in an irreversible manner. ..
  60. Poterszman A, Andersen G, Busso D, Rossignol M, Egly J, Thierry J. Expression in Escherichia coli: purification and characterization of cyclin H, a subunit of the human general transcription/DNA repair factor TFIIH. Protein Expr Purif. 1997;9:153-8 pubmed
    The human cyclin H, a protein normally associated with the cyclin-dependent kinase cdk7, was overexpressed in Escherichia coli using a T7 RNA polymerase expression system and further purified to apparent homogeneity...
  61. Koch S, Lamb F, Hellman J, Sherwood E, Stark R. Potentiation and tolerance of toll-like receptor priming in human endothelial cells. Transl Res. 2017;180:53-67.e4 pubmed publisher
    ..These results demonstrate that in human endothelial cells, prior activation of TLRs can have a significant impact on subsequent exposures and may contribute to the severity of the host response. ..
  62. Lin D, Fang H, Ma A, Huang Y, Pu Y, Jenster G, et al. Negative modulation of androgen receptor transcriptional activity by Daxx. Mol Cell Biol. 2004;24:10529-41 pubmed
    ..Together, these findings not only provide a novel role of Daxx in controlling AR transactivation activity but also uncover the mechanism underlying sumoylation-dependent transcriptional repression of the AR. ..
  63. Patel H, Abduljabbar R, Lai C, Periyasamy M, Harrod A, Gemma C, et al. Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor-Positive Breast Cancer. Clin Cancer Res. 2016;22:5929-5938 pubmed required for the regulation of the cell cycle and is a trimeric complex consisting of cyclin-dependent kinase 7 (CDK7), Cyclin H, and the accessory protein, MAT1...
  64. Ford B, Ruttan C, Kyle V, Brackley M, Glickman B. Identification of single nucleotide polymorphisms in human DNA repair genes. Carcinogenesis. 2000;21:1977-81 pubmed
    ..HSP may be used for any sequence that can be amplified by PCR. A total of 12 SNP variants in MGMT, ERCC1, CDK7, CCNH and XRCC4 were identified, 11 at polymorphic frequencies, with an average frequency of 0...
  65. Wenz C, Faust D, Linz B, Turmann C, Nikolova T, Bertin J, et al. t-BuOOH induces ferroptosis in human and murine cell lines. Arch Toxicol. 2018;92:759-775 pubmed publisher
    ..We identify that t-BuOOH and H2O2 produce a different pattern of lipid peroxidation, thereby leading to different cell death pathways and present t-BuOOH as a novel inducer of ferroptosis. ..
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