CDK5RAP3

Summary

Gene Symbol: CDK5RAP3
Description: CDK5 regulatory subunit associated protein 3
Alias: C53, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114, PP1553, CDK5 regulatory subunit-associated protein 3, CDK5 regulatory subunit associated protein IC53-2, LXXLL/leucine-zipper-containing ARF-binding protein, LXXLL/leucine-zipper-containing ARFbinding protein, ischemic heart CDK5 activator-binding protein C53
Species: human
Products:     CDK5RAP3

Top Publications

  1. Ching Y, Qi Z, Wang J. Cloning of three novel neuronal Cdk5 activator binding proteins. Gene. 2000;242:285-94 pubmed
    ..The full-length forms of these three novel proteins, designated C42, C48 and C53, have a molecular mass of 66, 24, and 57 kDa, respectively...
  2. Wu J, Lei G, Mei M, Tang Y, Li H. A novel C53/LZAP-interacting protein regulates stability of C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and modulates NF-kappaB signaling. J Biol Chem. 2010;285:15126-36 pubmed publisher
    C53/LZAP (also named as Cdk5rap3) is a putative tumor suppressor that plays important roles in multiple cell signaling pathways, including DNA damage response and NF-kappaB signaling...
  3. Kwon J, Cho H, Han S, No J, Kwon J, Kim H. A novel LZAP-binding protein, NLBP, inhibits cell invasion. J Biol Chem. 2010;285:12232-40 pubmed publisher
    LXXLL/leucine zipper-containing alternative reading frame (ARF)-binding protein (LZAP) was recently shown to function as a tumor suppressor through inhibition of the NF-kappaB signaling pathway...
  4. Mak G, Chan M, Leong V, Lee J, Yau T, Ng I, et al. Overexpression of a novel activator of PAK4, the CDK5 kinase-associated protein CDK5RAP3, promotes hepatocellular carcinoma metastasis. Cancer Res. 2011;71:2949-58 pubmed publisher
    The CDK5 kinase regulatory subunit-associated protein 3 (CDK5RAP3 or C53/LZAP) regulates apoptosis induced by genotoxic stress...
  5. Shiwaku H, Yoshimura N, Tamura T, Sone M, Ogishima S, Watase K, et al. Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J. 2010;29:2446-60 pubmed publisher
    ..Maxer is an endoplasmic reticulum (ER) membrane protein interacting with CDK5RAP3. Maxer anchors CDK5RAP3 to the ER and inhibits its function of Cyclin D1 transcription repression in the nucleus...
  6. Jiang H, Luo S, Li H. Cdk5 activator-binding protein C53 regulates apoptosis induced by genotoxic stress via modulating the G2/M DNA damage checkpoint. J Biol Chem. 2005;280:20651-9 pubmed
    ..Here we report the involvement of Cdk5 activator p35-binding protein C53 in regulation of apoptosis induced by genotoxic stress through modulating Cdk1-cyclin B1 function...
  7. Wang J, He X, Luo Y, Yarbrough W. A novel ARF-binding protein (LZAP) alters ARF regulation of HDM2. Biochem J. 2006;393:489-501 pubmed
    ..Using a yeast two-hybrid screen, we have identified a novel protein, LZAP (LXXLL/leucine-zipper-containing ARF-binding protein), that interacts with endogenous ARF in mammalian cells...
  8. Jiang H, Wu J, He C, Yang W, Li H. Tumor suppressor protein C53 antagonizes checkpoint kinases to promote cyclin-dependent kinase 1 activation. Cell Res. 2009;19:458-68 pubmed publisher
    ..We originally reported that a novel protein C53 (also known as Cdk5rap3 and LZAP) potentiates DNA damage-induced cell death by modulating the G2/M checkpoint. More recently, Wang et al...
  9. Wang J, An H, Mayo M, Baldwin A, Yarbrough W. LZAP, a putative tumor suppressor, selectively inhibits NF-kappaB. Cancer Cell. 2007;12:239-51 pubmed
    b>LZAP has been reported to inhibit cellular proliferation and clonogenic growth. Here, we report that decreased LZAP expression promoted cellular transformation, xenograft tumor growth, and xenograft tumor vascularity...

More Information

Publications44

  1. Yin X, Warner D, Roberts E, Pisano M, Greene R. Novel interaction between nuclear co-activator CBP and the CDK5 activator binding protein - C53. Int J Mol Med. 2005;16:251-6 pubmed
    ..The carboxy terminal region of CBP (including the C/H3 region) was utilized as a bait. C53, a 57 kDa protein known to bind to the p25 activator of cyclin-dependent kinase 5, was identified as a novel ..
  2. Stang A, Hawk H, Knowlton R, Gershman S, Kuss O. Hysterectomy-corrected incidence rates of cervical and uterine cancers in Massachusetts, 1995 to 2010. Ann Epidemiol. 2014;24:849-54 pubmed publisher
    ..We used incidence data of invasive cervical (International Classification of Diseases for Oncology, Third Edition: C53) and uterine cancer (International Classification of Diseases for Oncology, Third Edition: C54-C55) diagnosed from ..
  3. Ream T, Haag J, Pontvianne F, Nicora C, Norbeck A, Paša Tolić L, et al. Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit. Nucleic Acids Res. 2015;43:4163-78 pubmed publisher
    ..Two genes encode homologs of the yeast C53 subunit and either protein can assemble into Pol III...
  4. Zheng C, Wang J, Lin M, Zhang P, Liu L, Lin J, et al. CDK5RAP3 suppresses Wnt/β-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. J Exp Clin Cancer Res. 2018;37:59 pubmed publisher
    b>CDK5RAP3 was initially isolated as a binding protein of the CDK5 activator p35...
  5. Dráberová E, D Agostino L, Caracciolo V, Sládková V, Sulimenko T, Sulimenko V, et al. Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol. 2015;74:723-42 pubmed publisher
    ..of GCP2 antagonized the inhibitory effect of the CDK5 regulatory subunit-associated tumor suppressor protein 3 (C53) on DNA damage G2/M checkpoint activity...
  6. Tartaglione L, Mazzeo A, Dell Aversano C, Forino M, Giussani V, Capellacci S, et al. Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS. Anal Bioanal Chem. 2016;408:915-32 pubmed publisher
    ..the presence of a hydroxyl group at C44 (in OVTX-j1 and OVTX-k) and the lack of a hydroxyl group in the region C53-C78 (in OVTX-i and OVTX-j1). The toxin content of the analyzed Ostreopsis sp. strains was in the range 0.06-2...
  7. Singh Y, Gupta N, Verma V, Goel M, Gupta R. Selective disruption of disulphide bonds lowered activation energy and improved catalytic efficiency in TALipB from Trichosporon asahii MSR54: MD simulations revealed flexible lid and extended substrate binding area in the mutant. Biochem Biophys Res Commun. 2016;472:223-30 pubmed publisher
    ..DIANNA server predicted three disulphide bridges C53-C195 (S1), C89-C228 (S2) and C164-C254 (S3) in the enzyme...
  8. Lin K, Kao S, Lai C, Chen C, Wu C, Hsu H, et al. Tumor Suppressor Lzap Suppresses Wnt/β-Catenin Signaling to Promote Zebrafish Embryonic Ventral Cell Fates via the Suppression of Inhibitory Phosphorylation of Glycogen Synthase Kinase 3. J Biol Chem. 2015;290:29808-19 pubmed publisher
    ..However, regulations of this signaling pathway are not completely understood. Here, we report that Lzap, a tumor suppressor, controls nuclear translocation of β-catenin...
  9. Codas M, Pesch B, Adolphs M, Madrazo C, Matthias C, Heinze E, et al. Cancer mortality in Itapúa--A rural province of Paraguay 2003-2012. Cancer Epidemiol. 2016;40:1-6 pubmed publisher
    ..1%) were often not specified. The uterus (mainly the cervix) (C53-C55) was the leading cancer site in women with AMRWs of 17.2/100,000 in central and 14...
  10. Miller C, Cai Y, Patton T, Graves S, Li H, Sabbatini M. RCAD/BiP pathway is necessary for the proper synthesis of digestive enzymes and secretory function of the exocrine pancreas. Am J Physiol Gastrointest Liver Physiol. 2017;312:G314-G326 pubmed publisher
    ..Among the components of the Ufm1 system, Regulator of C53 and DDRGK1 (RCAD) has recently been identified as a Ufm1-specific E3 ligase that promotes ufmylation of DDRGK1, an ..
  11. Wamsley J, Gary C, Biktasova A, Hajek M, Bellinger G, Virk R, et al. Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner. Oncogenesis. 2017;6:e314 pubmed publisher
    ..Here, we show that leucine zipper-containing ARF-binding protein (LZAP) binds and stabilizes p53...
  12. Wamsley J, Issaeva N, An H, Lu X, Donehower L, Yarbrough W. LZAP is a novel Wip1 binding partner and positive regulator of its phosphatase activity in vitro. Cell Cycle. 2017;16:213-223 pubmed publisher
    ..Wip1 also dephosphorylates and inactivates RelA. Notably, LZAP, a putative tumor suppressor, has been linked to dephosphorylation of several of these substrates, including RelA, ..
  13. Hořejší B, Vinopal S, Sladkova V, Dráberová E, Sulimenko V, Sulimenko T, et al. Nuclear ?-tubulin associates with nucleoli and interacts with tumor suppressor protein C53. J Cell Physiol. 2012;227:367-82 pubmed publisher
    ..nuclear extracts combined with mass spectrometry revealed an association of ?-tubulin with tumor suppressor protein C53 located at multiple subcellular compartments including nucleoli...
  14. Zheng S, Wang L, Ren L, Luo J, Liao W, Liu E. Epidemiological analysis and follow-up of human rhinovirus infection in children with asthma exacerbation. J Med Virol. 2018;90:219-228 pubmed publisher
    ..001 and P?=?0.036). The main genotypes were HRV-C53 and HRV-A20 among inpatients, and this genotype caused more severe clinical manifestations...
  15. Cen H, Zheng S, Fang Y, Tang X, Dong Q. Induction of HSF1 expression is associated with sporadic colorectal cancer. World J Gastroenterol. 2004;10:3122-6 pubmed
    ..The induction of HSF1 gene expression is associated with sporadic colon cancer. HSF1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer. ..
  16. Stav D, Bar I, Sandbank J. Usefulness of CDK5RAP3, CCNB2, and RAGE genes for the diagnosis of lung adenocarcinoma. Int J Biol Markers. 2007;22:108-13 pubmed
    ..We analyzed CDK5RAP3 and CCNB2, which are involved in cell cycle progression, and RAGE...
  17. Chen J, Liu B, Liu Y, Han Y, Yu H, Zhang Y, et al. A novel gene IC53 stimulates ECV304 cell proliferation and is upregulated in failing heart. Biochem Biophys Res Commun. 2002;294:161-6 pubmed
    ..A novel gene with 84% homolog to C53, named IC53, was cloned from our 5300 EST database of human aorta cDNA library (GenBank Accession No. AF110322)...
  18. Zhuo M, Huang Y, Chen J, Sun L, Yang R, Chen H, et al. Endothelium-specific overexpression of human IC53 downregulates endothelial nitric oxide synthase activity and elevates systolic blood pressure in mice. Cardiovasc Res. 2009;84:292-9 pubmed publisher
    ..Endothelial cells (ECs) exert important functions in the regulation of blood pressure. A novel gene, IC53, as an isoform of the cyclin-dependent kinase (CDK)-binding protein gene C53, is mainly expressed in vascular ECs ..
  19. Li H, Li J, Lü C, Xia Y, Xin Y, Liu H, et al. FisR activates ?54 -dependent transcription of sulfide-oxidizing genes in Cupriavidus pinatubonensis JMP134. Mol Microbiol. 2017;105:373-384 pubmed publisher
    ..When polysulfide reacts with the R domain of FisR through the three conserved cysteine residues (C53, C64 and C71), FisR activates the expression of the operon...
  20. Wang J, Wang Z, Li Y, Huang C, Zheng C, Li P, et al. CDK5RAP3 acts as a tumor suppressor in gastric cancer through inhibition of ?-catenin signaling. Cancer Lett. 2017;385:188-197 pubmed publisher
    b>CDK5RAP3 was isolated as a binding protein of the Cdk5 activator p35. Although CDK5RAP3 has been implicated in cancer progression, its expression and function have not been investigated in gastric cancer...
  21. Lei Y, Liu H, Yang Y, Wang X, Ren N, Li B, et al. Interaction of LHBs with C53 promotes hepatocyte mitotic entry: A novel mechanism for HBV-induced hepatocellular carcinoma. Oncol Rep. 2012;27:151-9 pubmed publisher
    ..Sequencing of the positive clones identified the full-length cDNA of the C53 gene...
  22. Han M, Wang H, Zhang H, Han Z. Expression of TIP-1 confers radioresistance of malignant glioma cells. PLoS ONE. 2012;7:e45402 pubmed publisher
    ..yeast two-hybrid screening identified that TIP-1, through its single PDZ domain, binds to the carboxyl terminus of LZAP that has been studied as one tumor suppressor functioning through ARF binding and p53 activation...
  23. Mak G, Lai W, Zhou Y, Li M, Ng I, Ching Y. CDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cells. PLoS ONE. 2012;7:e42210 pubmed publisher
    b>CDK5 regulatory subunit associated protein 3 (CDK5RAP3) is a novel activator of PAK4 and processes important pro-metastatic function in hepatocarcinogenesis...
  24. Wang X, Ching Y, Lam W, Qi Z, Zhang M, Wang J. Identification of a common protein association region in the neuronal Cdk5 activator. J Biol Chem. 2000;275:31763-9 pubmed
    ..have been shown to undergo high affinity association with Nck5a, including three novel proteins, C42, C48, and C53 found by a yeast two-hybrid screen (Ching, Y. P., Qi, Z., and Wang, J. H. (2000) Gene 242, 285-294)...
  25. Zhang Y, Zhang M, Wu J, Lei G, Li H. Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking. PLoS ONE. 2012;7:e48587 pubmed publisher
    ..present the evidence for involvement of RCAD/Ufl1, a putative Ufm1-specific E3 ligase, and its binding partner C53/LZAP protein in ufmylation of endogenous Ufm1 targets...
  26. Chen J, Shi Y, Li Z, Yu H, Han Y, Wang X, et al. A functional variant of IC53 correlates with the late onset of colorectal cancer. Mol Med. 2011;17:607-18 pubmed publisher
    The IC53 gene was reported to be upregulated in the colon adenocarcinoma cell line SW480. Here, we show that the expression level of IC53 is positively correlated with the grade and depth of invasion in adenocarcinoma of the colon...
  27. An H, Lu X, Liu D, Yarbrough W. LZAP inhibits p38 MAPK (p38) phosphorylation and activity by facilitating p38 association with the wild-type p53 induced phosphatase 1 (WIP1). PLoS ONE. 2011;6:e16427 pubmed publisher
    LZAP (Cdk5rap3, C53) is a putative tumor suppressor that inhibits RelA, Chk1 and Chk2 and activates p53...
  28. Zhao J, Pan K, Li J, Chen Y, Chen J, Lv L, et al. Identification of LZAP as a new candidate tumor suppressor in hepatocellular carcinoma. PLoS ONE. 2011;6:e26608 pubmed publisher
    b>LZAP was isolated as a binding protein of the Cdk5 activator p35. LZAP has been highly conserved during evolution and has been shown to function as a tumor suppressor in various cancers...
  29. Wu J, Jiang H, Luo S, Zhang M, Zhang Y, Sun F, et al. Caspase-mediated cleavage of C53/LZAP protein causes abnormal microtubule bundling and rupture of the nuclear envelope. Cell Res. 2013;23:691-704 pubmed publisher
    ..We found that the NE rupture was caused by caspase-mediated cleavage of C53/LZAP, a protein that has been implicated in various signaling pathways, including NF-?B signaling and DNA damage ..
  30. Steffens M, Leu C, Ruppert A, Zara F, Striano P, Robbiano A, et al. Genome-wide association analysis of genetic generalized epilepsies implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32. Hum Mol Genet. 2012;21:5359-72 pubmed publisher
    ..1, ZEB2 at 2q22.3, SCN1A at 2q24.3 and PNPO at 17q21.32. Further replication efforts are necessary to elucidate whether these positional candidate genes contribute to the heritability of the common GGE syndromes. ..
  31. Camargo L, Collura V, Rain J, Mizuguchi K, Hermjakob H, Kerrien S, et al. Disrupted in Schizophrenia 1 Interactome: evidence for the close connectivity of risk genes and a potential synaptic basis for schizophrenia. Mol Psychiatry. 2007;12:74-86 pubmed
  32. Kim E, Chen F, Wang C, Harrison L. CDK5 is a novel regulatory protein in PPARgamma ligand-induced antiproliferation. Int J Oncol. 2006;28:191-4 pubmed
    ..This is the first report of cdk5/p35 expression and kinase activity in colon cancer cells, which is associated with ciglitazone-induced antiproliferation in HT-29 cells. ..
  33. Xie Y, He X, Tang Y, Li J, Pan Z, Qin W, et al. Cloning and characterization of human IC53-2, a novel CDK5 activator binding protein. Cell Res. 2003;13:83-91 pubmed
    We have identified IC53-2, a human homologue of the rat C53 gene from a human placenta cDNA library (GeneBank Accession No.AF217982). IC53-2 can bind to the CDK5 activator p35 by in vitro association assay...
  34. Ching Y, Pang A, Lam W, Qi R, Wang J. Identification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitor. J Biol Chem. 2002;277:15237-40 pubmed
    ..The Pho85.Pho80 kinase complex has been shown to be the yeast functional homologue of the mammalian Cdk5/p35(nck5a) kinase. ..
  35. Egamberdieva D, Wirth S, Shurigin V, Hashem A, Abd Allah E. Endophytic Bacteria Improve Plant Growth, Symbiotic Performance of Chickpea (Cicer arietinum L.) and Induce Suppression of Root Rot Caused by Fusarium solani under Salt Stress. Front Microbiol. 2017;8:1887 pubmed publisher
    ..A combined inoculation of chickpea with M. ciceri IC53 and B...