CD9

..and at least one yet unidentified current component rather insensitive to changes in [K]o and to K+ channel blockers...

..These results are the first to demonstrate an association between CD63 and two other TM4SF members, CD9 and CD81 in 2 human melanoma cell lines...

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..by monoclonal antibody (mAb) M31-15 inhibiting cell motility and that the sequence of MRP-1 coincides with that of CD9. In the present study, plasmid was constructed in which human MRP-1/CD9 cDNA is expressed under the control of the ..

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..Evidence for constitutive CD9 homodimers was obtained using several cell lines, utilizing the following four methods: (1) spontaneous cross-..

..plasma membrane leads to their specific association with transmembrane-4 superfamily (TM4SF; tetraspanin) proteins (CD9, CD53, CD81, CD82, and CD151), as demonstrated by reciprocal co-immunoprecipitation and covalent cross-linking ..

..EWI-2 associated specifically with CD9 and CD81 but not with other tetraspanins or with integrins...

..All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and CD151, were found to incorporate [3H]palmitate...

..4 transmembrane domains, member of the tetraspan superfamily, is engaged, together with other tetraspans such as CD9, CD53, CD63, and CD82, in multimolecular complexes containing beta1 integrins and major histocompatibility complex ..

..raised and selected monoclonal antibodies (mAb) for their ability to immunoprecipitate a molecule associated with CD9. A unique mAb was identified which recognizes the complement regulator CD46 (membrane cofactor protein)...

CD81 and CD9, members of the transmembrane-4 superfamily (TM4SF; tetraspanins), form extensive complexes with other TM4SF proteins, integrins, and other proteins, especially in mild detergents...

Cluster-of-differentiation antigen 9 (CD9) protein, a member of the tetraspanin family, has been implicated in carcinogenesis of various human tumors...

..Here, we reveal the presence of tetraspanin-enriched microdomains (TEMs) containing the tetraspanins CD9, CD63, CD81, and CD82 at the plasma membrane...

..mild detergent, digitonin, dissociated most of these associated molecules, including integrins, from the tetraspans CD9, CD37, CD53, CD63, CD82, Co-029, Talla-1 and NAG-2...

..Therefore, the role of CD81 and other related tetraspanin, CD9, on the cell-to-cell fusion process mediated by HIV-1 was studied...

Motility related protein-1 (MRP-1) is a transmembrane glycoprotein that is identical to the CD9 antigen. In previous studies, we showed that various types of cultured tumor cells transfected with MRP-1/CD9 cDNA have low motility and ..

DRAP27, the monkey homolog of human CD9 antigen (DRAP27/CD9) and diphtheria toxin receptor (DTR) were expressed in mouse L cells...

..We have previously identified a 135-kDa molecule (CD9P-1) as a major molecular partner of CD9 in cancer cell lines...

..of the recently described leucocyte surface (glyco)-proteins with significant amino acid sequence similarity (human CD9, CD37, CD53, CD63, TAPA-1, CO-029 and R2 and several homologues of other species) are distinguished by the ..

It has recently been shown that several members of the tetraspan superfamily, including CD9 and CD63, associate with each other and with beta1 integrins...

b>CD9 is a membrane protein belonging to the tetraspanin family...

..The tetraspanin CD9 has been implicated in cell motility, metastasis, and sperm-egg fusion...

..8. Di-t-BHA is proposed as a lead reference compound for future synthesis of new antioxidants combining two potentially useful properties in the prevention of tissue damage after ischaemia reperfusion injury...

..Altogether, these results suggest that atypical PKC isoenzymes, including PKC-zeta, participate in the signal transduction pathways by which HIV infection results in the activation of NF-kappa B in human monocytic cells and macrophages...

..Keywords: SH-SY5Y; calcium channels; noradrenaline release; muscarinic receptor; nifedipine; w-conotoxin GVIA; pirenzepine;hexahydrosiladiphenidol..

..Keywords: Ifenprodil; voltage-activated calcium channels; N-methyl-D-aspartate (NMDA); polyamines; cultured hippocampal pyramidal neurones..

..The DHP binding domain can be reached by agonists and antagonists from the extracellular but not from the intracellular face of the membrane...

..The inhibition of Ca channel current by AA may be partly induced by superoxide radicals derived from AA oxidation. PGF2A also reduces Ca channel currents but probably by a separate mechanism via activation of a GTP binding protein...

..analysis we demonstrated that the stroma-dependent MM5 cells show a downregulation of the motility-related protein (MRP-I CD9) which might reflect the involvement of this molecule in the regulation of myeloma cell extravasation.

..These preliminary observations suggest that short-term treatment with beta-carotene may increase CD4+ cell counts in patients with AIDS who have greater than 10 cells/microliters...

..Metal cation linkage of two displaced bases would allow separate DNA strands to crosslink. Aggregation is proposed to result from the formation of an extended network of these crosslinks...

..However, symptomatic patients responded much better than the asymptomatic patients...

..These effects of blockers, osmotic agents, and voltage on the lifetime, as well as the lack of effect of voltage at low salt levels, are consistent with the occupancy hypothesis...

..The SIA method indicated the positive readings earlier than the WB1 assay. The present findings indicated that the SIA method was more specific and sensitive than the WB assay, and would be useful as a confirmatory test...

..These results are consistent with a localized presynaptic inhibitory effect of NPY on glutamate release in hippocampal neurones in culture...

..7. When neurones were loaded with cholera toxin (CTX) or pertussis toxin (PTX) through a patch pipette, PTX suppressed the Str response whereas CTX did not, suggesting that G, and/or Go might be involved in the Str-induced IK...

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..7. It is concluded that LBK increases electrogenic chloride secretion in Colony 29 epithelia through the generation of second messengers cyclic AMP and Ca2+, each of which may act on both apical and basolateral membranes...

..It seems likely that basolateral K+ channels (1) are subject to anionic gating ligands, and (2) depend on anions with respect to the rate of K+ transfer through an open K+ channel...

..Such incorporation might increase guideline acceptance by practicing clinicians...

..Diagnosis of primary HIV-1 infection was made. Recently, HIV-1 infection has been increasing in Japan. Consideration of this disease in differential diagnosis of acute febrile illness is necessary...

..It also had a more transient time course, was activated at more negative potentials, and resembled the previously described low-voltage-activated, T-type calcium current...

..Additionally, our data suggest that the tax and pol gene regions are conserved between HTLV-I and STLV-I strains found among these diverse species of non-human primates...

..l-1 Ba2+ to cells bathed in symmetric K Ringer's solution resulted in nearly complete inhibition of outward current. Charybdotoxin produced only minor effects on this current.(ABSTRACT TRUNCATED AT 250 WORDS)..

..The stoichiometric displacement parameter, Z, in Ba2+ buffer is three times the value in Mg2+ buffer, and is close to unity...

..Our results suggest that both compounds prevent the increase of topoisomerase II activity, involved in virus replication, after infection of cells with HIV-1...

..6. The data indicate that adenosine activates K+-conductance via an A2 receptor in mesangial cells; the activation of the K+-conductance, which is probably mediated by cyclic AMP led to a hyperpolarization of membrane voltage...

..5. In conclusion, the ability of some divalent cations to inhibit ecto-ATPase activity and to potentiate or inhibit contractile responses in the guinea-pig urinary bladder appear to be independent effects...

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..01). HIV-p24 antigen was measured in 10 of 11 seropositive men. The antigen was positive in four men, and negative in six.(ABSTRACT TRUNCATED AT 250 WORDS)..

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..2',4'-Dimethylbenzamil was also able to inhibit Na+ efflux (by 3.05 +/- 0.98 mmol (L cells X h)-1; mean +/- SEM of three experiments) suggesting the existence of Na+:Sr2+ exchange.(ABSTRACT TRUNCATED AT 250 WORDS)..

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..The reagent are stable at 37 degrees C for 4 days. The results indicated that our reagent is highly specific, sensitive and stable, it can be used for detection of HIV-1/2 infections...

..HIV strains were isolated from these 3 cases. These results suggest that reduction of RT inhibiting antibody levels correlate well with the success of HIV isolation and with progression of clinical manifestation...

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..An R-type residual current is not present in these cells...

..None of the HIV-1 markers, except for antibody, was detected in the samples from their children and placenta. These facts strongly suggest that there was no HIV-1 transmission from mother to child...

..In order to study the epidemiology of Borna disease virus (BDV) in Zunyi region and its surrounding regions in Guizhou province...

..In addition, an unexpected relaxant effect of high concentrations of glibenclamide may be explained by inhibition of the Ca channels...

..Similarly, normal human peripheral blood mononuclear cells and uninfected, transformed human T cells, were also negative in the assay.(ABSTRACT TRUNCATED AT 250 WORDS)..

..Also, development of peripheral neuropathy may occur at lower cumulative doses in these patient populations...

..There is no problem with sample instability and no need for cumbersome nucleic acid extraction. The test is validated for subtype B, but requires further studies for non-B subtypes...

..The results indicate that Na+ absorption in the coprodeum of the chicken embryo is more complex than in adult hens.(ABSTRACT TRUNCATED AT 250 WORDS)..

..CD detection was possible for M+ and M2+ and UV detection for M2+. (3) For M2+, the mobile phase, 0.8 mM Ce(III) + 0.1 mM SDS + 1 mM HNO3 and indirect UV detection were effective. The IC methods were applied to real samples...

..b...

..The sensitivity of the response to Ba2+ suggests that it is triggered by membrane depolarization. These results suggest that glial pHi is normally modulated by the level of local neuronal activity...

..The results with Cs+ and Ba2+ indicate that both high and low concentrations of carbachol decrease sinoatrial node discharge by activating the I(K,ACh) channels rather than by decreasing If...

..A second mechanism involving a high-affinity site for PAK-104P and which would concern molecules such as anthracyclines, calcein acetoxymethyl ester whose efflux depends on the presence of glutathione...

..Change in antigen level in response to antiviral therapy needs further investigation before it is used as a surrogate marker for clinical efficacy of antiviral therapy...

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..LC-MS-MS, therefore, appears to be better for the detection of trace components in complex mixtures...

..To evaluate changes in architecture, viral RNA, and viral protein over 6 months in lymph nodes from retroviral-naïve HIV-infected persons before and after commencing highly active antiretroviral therapy (HAART)...

..To analyze the immunophenotype and leukemia associated immunophenotype (LAIP) of leukemia cells from patients with acute myeloid leukemia (AML) in minimal residual disease (MRD) detection...

..The aim of this study was to investigate the adjuvant-carrier properties of the B ricin subunit (RTB) when fused to p24...

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..Based on the results of a study using both isotopic and nonisotopic in situ hybridization, we assign the gene coding for human TPO to chromosome region 2p25-->p24...

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..These results suggest that proline residues in the NH(2)-terminal capsid domain represent critical structure determinants for proper formation of functional virion cores and subsequent stages of early replication...

Antibodies to CD9, a member of the tetraspan transmembrane-protein family, selectively inhibit Canine distemper virus (CDV)-induced cell-cell fusion...

..The results obtained suggest that ELFA are very useful for an earlier diagnosis of HIV infection and re-test for false positive samples by other HIV diagnosis kits...

..In the present study, the role of tetraspanin CD9 in LPS-induced macrophage activation and lung inflammation was investigated in vitro and in vivo...

..All of these specimens were positive by Genscreen HIV Ag-Ab. Therefore, Genscreen HIV Ag-Ab that shorten the window period is a useful and reliable for HIV screening test, especially in case of primary infection...

..We tested the hypothesis that loss of Drosophila melanogaster p24 protein function causes a transcriptional response characteristic of ER stress activation...

..Only a single kinetic pattern is detected in NHP terminals, suggesting that mechanisms for secretion are not universally distributed in excitable cells...

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..076). SI phenotype was associated with the development of a decreased zidovudine susceptibility. A correlation between zidovudine-associated pol gene mutations and SI phenotype was detected at the 6-month time point...

..These findings indicate that disease progression correlates with the appearance of variant viruses that are more infective and cytopathic...

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..Collectively, the data provide strong evidence that Gig2 is indeed a novel IFN inducible gene and its expression is likely dependent on IRF7 upon Poly I:C or IFN...

We showed that CD9, a member of tetraspanin superfamily proteins, is expressed in a specific membrane microdomain, called "lipid raft," and is crucial for cell fusion during osteoclastogenesis after activation of the RANK/RANKL ..

..Since this domain is essential for p24 oligomerization and capsid cone formation, CyPA bound to the new sites might impair the regularity of the capsid cone and thus facilitate in vivo core disassembly after host infection...

..Thirty cross-reacting (false-positive) samples by PA test from blood donors were tested by DUO, and all of these were negative by DUO...

..7. It is concluded that exposure to CN and to anoxia induces suppression of the Na+ pump and activation of non-selective cation channels, probably due to an ATP decrease resulting mainly from consumption by the Na+ pump...

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..To our knowledge, this is the first report of a member of the Hsp60 family priming for antigen-specific CTL activity when employed as a fusion protein partner...

b>CD9 (p24) and PECAM1 (CD31) antigens are well-defined components of the platelet plasma membrane. Both are integral glycoproteins (GPs) implicated in the adhesive and aggregative properties of human platelets...

..These results demonstrated that isolates from patients treated with AZT contained AZT-resistant variants which could replicate in the presence of 1.0 microM AZT...

..0 microM AZT and HIV-1 p24 antigen was detected. These results demonstrated that AZT-resistant variant was still a dominant population in the isolate from patients after 11 to 13 months of discontinuing AZT therapy...

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..This assay will also be used for VL testing of HIV-l Clade B-infected individuals as a first step towards a commercially viable assay. PROPOSED COMMERCIAL APPLICATION: Not Available ..

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..The proposed study provides a novel strategy to induce strong mucosal immunity against HIV-1 which can lead to development of an anti-HlV-1 oral vaccine due to its safety, low production cost and easy administration. ..

..The project is expected to provide important information for use in improving AML treatment stratification and outcomes. ..

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..This award will support the pursuit of my long-term career goal, which is to become a proficient independent clinical investigator focusing on infections in the immunocompromised host. ..

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..These relationships will be evaluated in the context of the clinical status of each patient group to be studied, and in comparison to HIV-uninfected subjects. ..

..of genes regulating the platelet response to specific agonists (ADP, CRP, collagen, TRAP, U46619, ristocetin, CD9 and epinephrine), as well as genes common to multiple platelet stimuli, and permit assessment of the effects of ..

..III) We will analyze protection levels achieved by DNA immunization with the modified Gag-Pol fusion constructs against intrarectal challenge with rVV expressing Gag and Pol proteins. ..

Transmembrane-4 superfamily (TM4SF) proteins such as CD9, CD63, CD81 and CD82 regulate cell invasion, motility, fusion, signaling, and proliferation. The mechanisms whereby these proteins function are largely unknown...