CASS4

Summary

Gene Symbol: CASS4
Description: Cas scaffolding protein family member 4
Alias: C20orf32, CAS4, HEFL, HEPL, HEF-like protein, HEF1-EFS-p130Cas-like protein, cas scaffolding protein family member 4
Species: human

Top Publications

  1. doi Toroidal Structure and DNA Cleavage by the CRISPR-Associated [4Fe-4S] Cluster Containing Cas4 Nuclease SSO0001 from Sulfolobus solfataricus
    Sofia Lemak
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada
    J Am Chem Soc 135:17476-87. 2013
  2. ncbi [Classical taxomomies]
    G Iu Liubarskiĭ
    Zh Obshch Biol 67:389-96. 2006
  3. pmc Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax
    André Plagens
    Institute of Biology, Microbiology I, University of Duisburg Essen, Essen, Germany
    J Bacteriol 194:2491-500. 2012
  4. doi Overexpression and cytoplasmic accumulation of Hepl is associated with clinicopathological parameters and poor prognosis in non-small cell lung cancer
    Yuan Miao
    Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China
    Tumour Biol 34:107-14. 2013
  5. pmc The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster
    Jing Zhang
    Biomedical Sciences Research Complex, University of St Andrews, Fife, United Kingdom
    PLoS ONE 7:e47232. 2012
  6. pmc Characterization of CRISPR RNA biogenesis and Cas6 cleavage-mediated inhibition of a provirus in the haloarchaeon Haloferax mediterranei
    Ming Li
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    J Bacteriol 195:867-75. 2013
  7. pmc The CRISPR-associated gene cas2 of Legionella pneumophila is required for intracellular infection of amoebae
    FELIZZA F GUNDERSON
    Department of Microbiology and Immunology, Northwestern University, Medical School, Chicago, Illinois, USA
    MBio 4:e00074-13. 2013
  8. doi Novel configurations of type I and II CRISPR-Cas systems in Corynebacterium diphtheriae
    Vartul Sangal
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
    Microbiology 159:2118-26. 2013
  9. pmc NEDD9 and BCAR1 negatively regulate E-cadherin membrane localization, and promote E-cadherin degradation
    Nadezhda Tikhmyanova
    Developmental Therapeutics, Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 6:e22102. 2011
  10. doi Characterization of the multiple CRISPR loci on Streptomyces linear plasmid pSHK1
    Peng Guo
    Key Laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
    Acta Biochim Biophys Sin (Shanghai) 43:630-9. 2011

Scientific Experts

  • André Plagens
  • Alexander Bolotin
  • Daniel H Haft
  • Rund Jansen
  • Nadezhda Tikhmyanova
  • Erica A Golemis
  • Sofia Lemak
  • FELIZZA F GUNDERSON
  • Yuan Miao
  • Ming Li
  • Vartul Sangal
  • Jing Zhang
  • Peter Simeonov
  • Marlene Cabrine-Santos
  • Peng Guo
  • Mahendra K Singh
  • Yung Chin Hsiao
  • Natalia Cymrot Cymbalista
  • G Iu Liubarskiĭ
  • F Guerra
  • Di Liu
  • Hong Yi Cao
  • Nicholas P Cianciotto
  • Jing Han
  • Rui Wang
  • Natalia Beloglazova
  • Greg Brown
  • Jingfang Liu
  • Hua Xiang
  • Andrzej Joachimiak
  • Alexander F Yakunin
  • Dahe Zhao
  • Ai lin Li
  • Tatiana Skarina
  • Ana Popović
  • Liang Wang
  • Shu Li Liu
  • Boguslaw Nocek
  • Robert Flick
  • Xiu Peng Zhang
  • Yang Liu
  • Hailong Liu
  • En Hua Wang
  • Alexei Savchenko
  • Paul A Hoskisson
  • Gui Yang Jiang
  • Peter C Fineran
  • Jian Zhou
  • Taciana Kasciukovic
  • Malcolm F White
  • Qiuxiang Cheng
  • Ralf Hoffmann
  • Bruna Ferreira de Souza
  • André Luiz Pedrosa
  • Yun Fan
  • Pengfei Xie
  • Eliane Lages-Silva
  • Zhongjun Qin
  • Norbert Strater
  • Thole Zuchner
  • Renate Berger-Hoffmann
  • Weihong Jiang
  • Luis Eduardo Ramirez
  • Alexei V Tulin
  • Fabrice Roegiers
  • Joy L Little
  • Brian L Egleston
  • Ilya G Serebriiskii
  • Emmanuelle Nicolas
  • Disha Dadke
  • Sinoula Apostolou
  • Adrian Canutescu
  • Shun Fa Yang
  • Yih Shou Hsieh
  • Hui Ling Chiou
  • Shu Chen Chu
  • Wu Hsien Kuo
  • Whei Ling Chiang
  • Zilda Najjar Prado de Oliveira
  • E Almeda
  • J C Daza
  • D Nandi
  • M Yoshino
  • M N Iyer
  • J J Monaco
  • K Moriwaki
  • T Sagai
  • K F Lindahl
  • T Shiroishi
  • Y Toyoda

Detail Information

Publications23

  1. doi Toroidal Structure and DNA Cleavage by the CRISPR-Associated [4Fe-4S] Cluster Containing Cas4 Nuclease SSO0001 from Sulfolobus solfataricus
    Sofia Lemak
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada
    J Am Chem Soc 135:17476-87. 2013
    b>Cas4 proteins, a core protein family associated with the microbial system of adaptive immunity CRISPR, are predicted to function in the adaptation step of the CRISPR mechanism...
  2. ncbi [Classical taxomomies]
    G Iu Liubarskiĭ
    Zh Obshch Biol 67:389-96. 2006
    ..e., a theory of meron organization that woud hepl establish the relationship of taxonomic systems developed for different merons.
  3. pmc Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax
    André Plagens
    Institute of Biology, Microbiology I, University of Duisburg Essen, Essen, Germany
    J Bacteriol 194:2491-500. 2012
    ..CRISPR/Cas subtype I-A, and the core cas genes are shown to be arranged on two polycistronic transcripts: cascis (cas4, cas1/2, and csa1) and cascade (csa5, cas7, cas5a, cas3, cas3', and cas8a2)...
  4. doi Overexpression and cytoplasmic accumulation of Hepl is associated with clinicopathological parameters and poor prognosis in non-small cell lung cancer
    Yuan Miao
    Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China
    Tumour Biol 34:107-14. 2013
    b>Hepl, first described in 2008, is the fourth member of the Crk-associated substrate (CAS) family and is specifically expressed in the lung...
  5. pmc The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster
    Jing Zhang
    Biomedical Sciences Research Complex, University of St Andrews, Fife, United Kingdom
    PLoS ONE 7:e47232. 2012
    The Cas4 protein is one of the core CRISPR-associated (Cas) proteins implicated in the prokaryotic CRISPR system for antiviral defence...
  6. pmc Characterization of CRISPR RNA biogenesis and Cas6 cleavage-mediated inhibition of a provirus in the haloarchaeon Haloferax mediterranei
    Ming Li
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    J Bacteriol 195:867-75. 2013
    ..With genetic approaches, we discovered that the lack of Cas1, Cas3, or Cas4 unexpectedly resulted in a decrease of CRISPR transcripts, while Cas5, Cas6, and Cas7 were found to be essential in ..
  7. pmc The CRISPR-associated gene cas2 of Legionella pneumophila is required for intracellular infection of amoebae
    FELIZZA F GUNDERSON
    Department of Microbiology and Immunology, Northwestern University, Medical School, Chicago, Illinois, USA
    MBio 4:e00074-13. 2013
    ..pneumophila strain 130b chromosome, we detected a subtype II-B CRISPR-Cas locus that contains cas9, cas1, cas2, cas4, and an array with 60 repeats and 58 unique spacers...
  8. doi Novel configurations of type I and II CRISPR-Cas systems in Corynebacterium diphtheriae
    Vartul Sangal
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
    Microbiology 159:2118-26. 2013
    ..The type II-C system was the most common (11/17 isolates) but it lacked the csn2 and cas4 genes that are involved in spacer acquisition...
  9. pmc NEDD9 and BCAR1 negatively regulate E-cadherin membrane localization, and promote E-cadherin degradation
    Nadezhda Tikhmyanova
    Developmental Therapeutics, Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 6:e22102. 2011
    The Cas scaffolding proteins (NEDD9/HEF1/CAS-L, BCAR1/p130Cas, EFSSIN, and HEPL/CASS4) regulate cell migration, division and survival, and are often deregulated in cancer...
  10. doi Characterization of the multiple CRISPR loci on Streptomyces linear plasmid pSHK1
    Peng Guo
    Key Laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
    Acta Biochim Biophys Sin (Shanghai) 43:630-9. 2011
    ..CRISPR-associated) genes were found, including a cluster of eight cas genes, in the order cas2B-cas1B-cas3B-cas5-cas4-cas2A-cas1A-cas3A, bracketed by a pair of divergent CRISPRs, and five other dispersed CRISPRs...
  11. doi Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield
    Peter Simeonov
    Ultrasensitive Protein Detection Unit, Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany
    Protein Eng Des Sel 24:261-8. 2011
    ..Recombinant human enteropeptidase light chain (hEPL) shows high activity but low solubility and refolding yields, currently limiting its use in biotechnological ..
  12. doi Sequencing and analysis of chromosomal extremities of Trypanosoma rangeli in comparison with Trypanosoma cruzi lineages
    Marlene Cabrine-Santos
    Discipline of Parasitology, Department of Biological Sciences, Federal University of Triangulo Mineiro, 38025 180, Uberaba, Minas Gerais, Brazil
    Parasitol Res 108:459-66. 2011
    ..The GTT triplet was detected in all KP1(+) strains, except for strain Cas4, but not in any of the KP1(-) strains. The dendrogram constructed by alignment of all T...
  13. pmc Dcas supports cell polarization and cell-cell adhesion complexes in development
    Nadezhda Tikhmyanova
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 5:e12369. 2010
    ..the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development...
  14. ncbi Molecular and serological analysis of polymorphisms in the murine major histocompatibility complex-encoded proteasome subunits, LMP-2 and LMP-7
    D Nandi
    Howard Hughes Medical Institute, University of Cincinnati, OH 45267 0524, USA
    Exp Clin Immunogenet 13:20-9. 1996
    ..Interestingly, a single amino acid change at position 272 between LMP-7b,d,q and LMP-7k,s,f,x,g7, cas4 from glycine to arginine dramatically affects its migration on SDS-PAGE gels, suggesting the possibility of allele-..
  15. ncbi Identification of genes that are associated with DNA repeats in prokaryotes
    Ruud Jansen
    Department of Infectious Diseases and Immunology, Bacteriology Division, Veterinary Faculty, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Mol Microbiol 43:1565-75. 2002
    ..The cas3 gene showed motifs characteristic for helicases of the superfamily 2, and the cas4 gene showed motifs of the RecB family of exonucleases, suggesting that these genes are involved in DNA metabolism ..
  16. ncbi Immunotherapy with a depigmented, polymerized vaccine of Olea europaea pollen allergens. Significantly reduces specific bronchial and skin test reactivity in sensitized patients after one year of treatment
    F Guerra
    Servicio de Alergia, Hospital Universitario Reina Sofia, Cordoba, Spain
    J Investig Allergol Clin Immunol 13:108-17. 2003
    ..a maximum concentration of 44 micrograms/ml of the depigmented, polymerized allergen extract (equivalent to 100 HEPL of the native unmodified extract)...
  17. ncbi Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin
    Alexander Bolotin
    Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    Microbiology 151:2551-61. 2005
    ..CRISPR structures are found in the vicinity of four genes named cas1 to cas4. In silico analysis revealed another cluster of three genes associated with CRISPR structures in many bacterial ..
  18. pmc A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes
    Daniel H Haft
    The Institute for Genomic Research, Rockville, Maryland, USA
    PLoS Comput Biol 1:e60. 2005
    ..Four CRISPR-associated (Cas) protein families, designated Cas1 to Cas4, are strictly associated with CRISPR elements and always occur near a repeat cluster...
  19. ncbi Treatment of idiopathic cutaneous hyperchromia of the orbital region (ICHOR) with intense pulsed light
    Natalia Cymrot Cymbalista
    Dermatology Division, Hospital das Clínicas of the Faculty of Medicine of the University of São Paulo, Sao Paulo, Brazil
    Dermatol Surg 32:773-83; discussion 783-4. 2006
    ..Idiopathic cutaneous hyperchromia of the orbital region (ICHOR) does not have a clear etiopathogenesis. Genetic factors, increased melanin, prominent vasculature, and eyelid skin slackness seem to be involved...
  20. ncbi The tumor-growth inhibitory activity of flavanone and 2'-OH flavanone in vitro and in vivo through induction of cell cycle arrest and suppression of cyclins and CDKs
    Yung Chin Hsiao
    Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan
    J Biomed Sci 14:107-19. 2007
    ..We determined that flavanone and 2'-OH flavanone inhibited cell growth of A549, LLC, AGS, SK-Hepl and HA22T cancer cells, while other flavanones showed little or no inhibition...
  21. pmc A novel Cas family member, HEPL, regulates FAK and cell spreading
    Mahendra K Singh
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Mol Biol Cell 19:1627-36. 2008
    ..We here report a previously undescribed novel branch of the Cas protein family, designated HEPL (for HEF1-Efs-p130Cas-like)...
  22. pmc CAS proteins in normal and pathological cell growth control
    Nadezhda Tikhmyanova
    Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA, 19111, USA
    Cell Mol Life Sci 67:1025-48. 2010
    Proteins of the CAS (Crk-associated substrate) family (BCAR1/p130Cas, NEDD9/HEF1/Cas-L, EFS/SIN and CASS4/HEPL) are integral players in normal and pathological cell biology...
  23. ncbi No dosage effect of recombinational hotspots in the mouse major histocompatibility complex
    M Yoshino
    Department of Cell Genetics, National Institute of Genetics, Mishima, Japan
    Immunogenetics 39:381-9. 1994
    ..The wm7 and cas3 haplotypes have a hotspot close to the Lmp-2 gene (Lmp-2 hotspot), and the cas4 haplotype has a hotspot about 100 kilobase (kb) proximal, close to the Pb gene (Pb hotspot)...