BUB1

Summary

Gene Symbol: BUB1
Description: BUB1 mitotic checkpoint serine/threonine kinase
Alias: BUB1A, BUB1L, hBUB1, BUB1 budding uninhibited by benzimidazoles 1 homolog, budding uninhibited by benzimidazoles 1 homolog, mitotic checkpoint serine/threonine-protein kinase BUB1, mitotic spindle checkpoint kinase
Species: human

Top Publications

  1. pmc Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer
    Gennadi V Glinsky
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Clin Invest 115:1503-21. 2005
  2. pmc Structure and substrate recruitment of the human spindle checkpoint kinase Bub1
    Jungseog Kang
    Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 32:394-405. 2008
  3. pmc The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores
    Victor M Bolanos-Garcia
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Structure 17:105-16. 2009
  4. pmc Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis
    Zhanyun Tang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 101:18012-7. 2004
  5. pmc Bub1 regulates chromosome segregation in a kinetochore-independent manner
    Christiane Klebig
    Institute of Biochemistry, Eidgenossische Technische Hochschule ETH Zurich, 8093 Zurich, Switzerland
    J Cell Biol 185:841-58. 2009
  6. ncbi Bub1 maintains centromeric cohesion by activation of the spindle checkpoint
    David Perera
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    Dev Cell 13:566-79. 2007
  7. ncbi Kinetochore localisation and phosphorylation of the mitotic checkpoint components Bub1 and BubR1 are differentially regulated by spindle events in human cells
    S S Taylor
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Cell Sci 114:4385-95. 2001
  8. ncbi The mitotic checkpoint protein hBUB3 and the mRNA export factor hRAE1 interact with GLE2p-binding sequence (GLEBS)-containing proteins
    X Wang
    Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 276:26559-67. 2001
  9. ncbi Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1
    Jeroen Pouwels
    Medical Biotechnology, VTT Technical Research Centre of Finland, Turku, Finland
    Cell Cycle 6:1579-85. 2007
  10. ncbi KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome
    Wei Qi
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 282:3672-9. 2007

Detail Information

Publications137 found, 100 shown here

  1. pmc Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer
    Gennadi V Glinsky
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Clin Invest 115:1503-21. 2005
    ....
  2. pmc Structure and substrate recruitment of the human spindle checkpoint kinase Bub1
    Jungseog Kang
    Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 32:394-405. 2008
    ..The checkpoint kinase Bub1 contributes to checkpoint sensitivity through phosphorylating the APC/C activator, Cdc20, and inhibiting APC/C ..
  3. pmc The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores
    Victor M Bolanos-Garcia
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Structure 17:105-16. 2009
    The interaction of the central mitotic checkpoint component BUB1 with the mitotic kinetochore protein Blinkin is required for the kinetochore localization and function of BUB1 in the mitotic spindle assembly checkpoint, the regulatory ..
  4. pmc Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis
    Zhanyun Tang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 101:18012-7. 2004
    ..Here, we report that Bub1 retains centromeric cohesion in mitosis of human cells...
  5. pmc Bub1 regulates chromosome segregation in a kinetochore-independent manner
    Christiane Klebig
    Institute of Biochemistry, Eidgenossische Technische Hochschule ETH Zurich, 8093 Zurich, Switzerland
    J Cell Biol 185:841-58. 2009
    The kinetochore-bound protein kinase Bub1 performs two crucial functions during mitosis: it is essential for spindle checkpoint signaling and for correct chromosome alignment...
  6. ncbi Bub1 maintains centromeric cohesion by activation of the spindle checkpoint
    David Perera
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    Dev Cell 13:566-79. 2007
    b>Bub1 is a component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures genome stability by delaying anaphase until all the chromosomes are stably attached to spindle microtubules via their kinetochores...
  7. ncbi Kinetochore localisation and phosphorylation of the mitotic checkpoint components Bub1 and BubR1 are differentially regulated by spindle events in human cells
    S S Taylor
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Cell Sci 114:4385-95. 2001
    b>BUB1 is a budding yeast gene required to ensure that progression through mitosis is coupled to correct spindle assembly...
  8. ncbi The mitotic checkpoint protein hBUB3 and the mRNA export factor hRAE1 interact with GLE2p-binding sequence (GLEBS)-containing proteins
    X Wang
    Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 276:26559-67. 2001
    ..Here we report that the two known binding partners of hBUB3, the mitotic checkpoint proteins hBUB1 and hBUBR1, both carry a region with remarkable similarity to the GLEBS motif of hNUP98...
  9. ncbi Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1
    Jeroen Pouwels
    Medical Biotechnology, VTT Technical Research Centre of Finland, Turku, Finland
    Cell Cycle 6:1579-85. 2007
    ..Furthermore our studies implicate Sgo1 in the tension sensing mechanism of the spindle checkpoint by regulating Plk1 kinetochore affinity...
  10. ncbi KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome
    Wei Qi
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 282:3672-9. 2007
    ..b>Bub1 is a protein serine-threonine kinase that plays multiple roles in chromosome segregation and the spindle checkpoint...
  11. ncbi Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20
    Christopher J Morrow
    Faculty of Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 118:3639-52. 2005
    ..Several other protein kinases, including Bub1 and members of the Ipl1/aurora family, also regulate anaphase onset...
  12. ncbi Genomic instability at the BUB1 locus in colorectal cancer, but not in non-small cell lung cancer
    R G Jaffrey
    Department of Medicine and Therapeutics, Institute of Medical Sciences, University of Aberdeen, Scotland
    Cancer Res 60:4349-52. 2000
    ..Mutations in the mitotic spindle checkpoint kinase BLUB1 cause a dominant negative disruption of the spindle, leading to chromosome instability in ..
  13. ncbi A double missense variation of the BUB1 gene and a defective mitotic spindle checkpoint in the pancreatic cancer cell line Hs766T
    Paula M Hempen
    Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD, USA
    Hum Mutat 21:445. 2003
    To determine sequence variations of the BUB1 and BUB1B genes in pancreatic cancer, the entire coding regions of the BUB1 and BUB1B genes were sequenced in pancreatic cancer cell lines and xenografts...
  14. pmc Surveillance mechanism linking Bub1 loss to the p53 pathway
    Ole V Gjoerup
    Molecular Virology Program, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    Proc Natl Acad Sci U S A 104:8334-9. 2007
    b>Bub1 is a kinase believed to function primarily in the mitotic spindle checkpoint. Mutation or aberrant Bub1 expression is associated with chromosomal instability, aneuploidy, and human cancer...
  15. ncbi Differential expression of the MAD2, BUB1 and HSP27 genes in Barrett's oesophagus-their association with aneuploidy and neoplastic progression
    Shareen H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Mutat Res 547:133-44. 2004
    ..Gene expression levels of the MAD2 and BUB1 mitotic spindle checkpoint genes were assessed in 37 Barrett's patients (with histology ranging from metaplasia to ..
  16. ncbi A role for the Adenomatous Polyposis Coli protein in chromosome segregation
    K B Kaplan
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Cell Biol 3:429-32. 2001
    ..APC localizes to the ends of microtubules embedded in kinetochores and forms a complex with the checkpoint proteins Bub1 and Bub3. In vitro, APC is a high-affinity substrate for Bub kinases...
  17. ncbi Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival
    Masayoshi Shichiri
    Department of Clinical and Molecular Endocrinology, Tokyo Medical and Dental University Graduate School, Bunkyo ku, Tokyo 113 8519, Japan
    Cancer Res 62:13-7. 2002
    Sequence alterations of mitotic checkpoint genes, hBUB1 and hBUBR1, were examined, and their gene transcripts were quantified using on-line, real-time quantitative reverse transcription-PCR in surgically resected human colorectal cancers ..
  18. ncbi Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression
    Victoria L Johnson
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Cell Sci 117:1577-89. 2004
    ..The protein kinase Bub1 localizes to the kinetochore very early during mitosis, followed by Cenp-F, BubR1, Cenp-E and finally Mad2...
  19. pmc A dual role for Bub1 in the spindle checkpoint and chromosome congression
    Patrick Meraldi
    Department of Biology and Biological Engineering, MIT, Cambridge, MA 02139 4307, USA
    EMBO J 24:1621-33. 2005
    ..In this study, we use live-cell imaging coupled with RNA interference to investigate the functions of human Bub1. We find that Bub1 is essential for checkpoint control and for correct chromosome congression...
  20. ncbi Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization
    Tomoya S Kitajima
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113 0032, Japan
    Curr Biol 15:353-9. 2005
    ..In fission yeast, Sgo localization depends on Bub1, a conserved spindle checkpoint protein, which is enigmatically also required for chromosome congression during ..
  21. pmc BUB1 mediation of caspase-independent mitotic death determines cell fate
    Yohei Niikura
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Cell Biol 178:283-96. 2007
    ..In BUB1-deficient (but not MAD2-deficient) cells, conditions that activate the spindle checkpoint (i.e...
  22. pmc The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase
    S S Taylor
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 142:1-11. 1998
    ..Previously, we showed that the murine homologue of Bub1 is not only required for checkpoint response to spindle damage, but also restrains progression through a normal ..
  23. ncbi The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis
    S A Jablonski
    Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Chromosoma 107:386-96. 1998
    ..Human cells contain two related protein kinases, hBUB1 and hBUBR1, that appear to have evolved from a single ancestral BUB1 gene...
  24. ncbi Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1
    Tomomi Kiyomitsu
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Dev Cell 13:663-76. 2007
    ..AF15q14/blinkin, a member of the Spc105/Spc7/KNL-1 family, directly links spindle checkpoint proteins BubR1 and Bub1 to kinetochores and is required for spindle checkpoint and chromosome alignment...
  25. ncbi Mutations of mitotic checkpoint genes in human cancers
    D P Cahill
    The Johns Hopkins Oncology Center, Program in Human Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Nature 392:300-3. 1998
    ..The normal mitotic checkpoints of cells displaying microsatellite instability become defective upon transfer of mutant hBUB1 alleles from either of two CIN cancers.
  26. ncbi hBUB1 defects in leukemia and lymphoma cells
    Hon Yu Ru
    Institute of Medical Science, Tzu Chi University, Hualien, Taiwan, Republic of China
    Oncogene 21:4673-9. 2002
    ..In some colorectal cancers with CIN it was found to have hBUB1 gene mutated and the mutated gene functions dominantly...
  27. ncbi Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint
    Zhanyun Tang
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 16:387-97. 2004
    ..Here we show that HeLa cells depleted for Bub1 by RNA interference are defective in checkpoint signaling...
  28. ncbi Characterization of MAD2B and other mitotic spindle checkpoint genes
    D P Cahill
    Program in Human Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21231, USA
    Genomics 58:181-7. 1999
    ..In addition, we resolved the genomic intron-exon structure of the human BUB1 gene. We then searched for mutations in these genes in a panel of 19 aneuploid colorectal tumors...
  29. doi Characterization of the tetratricopeptide-containing domain of BUB1, BUBR1, and PP5 proves that domain amphiphilicity over amino acid sequence specificity governs protein adsorption and interfacial activity
    Sylvie Beaufils
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    J Phys Chem B 112:7984-91. 2008
    ..The N-terminal region of BUB1, BUBR1, and protein phosphatase 5 (PP5) contains tandem arrangements of the TPR motif...
  30. ncbi Bub1 is activated by the protein kinase p90(Rsk) during Xenopus oocyte maturation
    M S Schwab
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado, School of Medicine, 4200 East Ninth Ave, Denver, CO 80262, USA
    Curr Biol 11:141-50. 2001
    ..In yeast, the serine/threonine protein kinase Bub1 and the WD-repeat protein Bub3 are elements of a signal transduction cascade that regulates the kinetochore ..
  31. pmc The function of a spindle checkpoint gene bub-1 in C. elegans development
    Xiangming Wang
    School of Life Sciences, Peking University, Beijing, China
    PLoS ONE 4:e5912. 2009
    The serine/threonine kinase BUB1 (Budding Uninhibited by Benzimidazole 1) was originally identified in yeast as a checkpoint protein, based on its mutant's incapacity of delaying the cell cycle in response to loss of microtubules...
  32. pmc A screen for dynein synthetic lethals in Aspergillus nidulans identifies spindle assembly checkpoint genes and other genes involved in mitosis
    V P Efimov
    Department of Pharmacology, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Genetics 149:101-16. 1998
    ..nidulans autonomously replicating vector. Sequencing revealed homology to the spindle assembly checkpoint genes BUB1 and BUB3 from Saccharomyces cerevisiae...
  33. ncbi mei-41 and bub1 block mitosis at two distinct steps in response to incomplete DNA replication in Drosophila embryos
    M Garner
    Molecular, Cellular, and Developmental Biology, University of Colorado, 80309, Boulder, CO, USA
    Curr Biol 11:1595-9. 2001
    ..We find that mitotic cyclins are stabilized and that a spindle checkpoint protein, Bub1, localizes onto chromosomes during mitotic arrest in dup mutants...
  34. pmc Ska3 is required for spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis
    John R Daum
    Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Curr Biol 19:1467-72. 2009
    ..Ska3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores. Ska3 protein accumulation at kinetochores in prometaphase is dependent on Sgo1 protein...
  35. pmc Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens
    Jingwei Cheng
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Semin Cancer Biol 19:218-28. 2009
    ..SV40 LT also binds to several additional cellular proteins including p300, CBP, Cul7, IRS1, Bub1, Nbs1 and Fbxw7 that contribute to viral transformation...
  36. ncbi Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast
    Yusuke Toyoda
    Department of Gene Mechanisms, Graduate School of Biostudies, 606 8502, Kyoto, Japan
    Curr Biol 12:347-58. 2002
    ..The failure to form cohesion in the S phase results in premature separation of chromatids in G2/M. Mitotic kinetochores free from microtubules or the lack of tension are known to activate spindle checkpoint...
  37. ncbi Modulation of cell cycle and gene expression in pancreatic tumor cell lines by methionine deprivation (methionine stress): implications to the therapy of pancreatic adenocarcinoma
    Demetrius M Kokkinakis
    Department of Pathology, University of Pittsburgh, PA 15213 1863, USA
    Mol Cancer Ther 4:1338-48. 2005
    ..of NF2, ETS2, CLU, GADD45alpha, GADD45beta, and GADD45gamma and down-regulation of AURKB, TOP2A, CCNA, CCNB, PRC1, BUB1, NuSAP, IFI16, and BRCA1...
  38. pmc Bub1 and CENP-F can contribute to Kaposi's sarcoma-associated herpesvirus genome persistence by targeting LANA to kinetochores
    Bingyi Xiao
    Department of Microbiology and Tumor Virology Program of the Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Virol 84:9718-32. 2010
    ..Moreover, LANA associated with another kinetochore protein, Bub1 (budding uninhibited by benzimidazole 1), which is known to form a complex with CENP-F...
  39. pmc Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension
    Katie B Shannon
    SPIRE fellow, Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Mol Biol Cell 13:3706-19. 2002
    ..decrease in tension at 23 degrees C, and metaphase kinetochores at 23 degrees C exhibited higher levels of 3F3/2, Bub1, and BubR1 compared with 37 degrees C...
  40. ncbi Inhibitors of histone deacetylases alter kinetochore assembly by disrupting pericentromeric heterochromatin
    April R Robbins
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA
    Cell Cycle 4:717-26. 2005
    ..Decreased amounts of hBUB1, CENP-F and the motor protein CENP-E were present on kinetochores of treated cells...
  41. ncbi The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo
    Jomon Joseph
    National Institutes of Health, National Institute of Child Health and Human Development, Building 18, Room 106, 18 Library Drive, MSC 5431, Bethesda, MD 20892, USA
    Curr Biol 14:611-7. 2004
    ..No similar disruption was observed after ablation of proteins nonessential for MT-kinetochore interactions (CENP-I, Bub1, CENP-E )...
  42. ncbi Genetic evidence for a role of phospholipase C at the budding yeast kinetochore
    N DeLillo
    Department of Biological Sciences, St John s University, 8000 Utopia Parkway, Jamaicam New York, NY 11439, USA
    Mol Genet Genomics 269:261-70. 2003
    ..However, plc1Delta cells require a functional BUB1/BUB3-dependent spindle checkpoint for viability...
  43. pmc Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)
    Gustavo Delhon
    Plum Island Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Greenport, New York 11944, USA
    J Virol 80:8439-49. 2006
    ....
  44. ncbi Sequence analysis of two cosmids from Schizosaccharomyces pombe chromosome III
    M Lucas
    Institut National Agronomique, Laboratoire de Genetique Moleculaire et Cellulaire, INRA, CNRS, Centre de Biotechnologies Agro Industrielles, 78850 Thiverval Grignon, France
    Yeast 16:1519-26. 2000
    ..gms1 on SPCC895, encoding the mating type P-factor precursor and an UDP-galactose transporter, respectively, and bub1 and ade6 in SPCC1322, encoding a protein kinase and a phosphoribosylaminoimidazole carboxylase, respectively...
  45. ncbi Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells
    Elsa Logarinho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Cell Sci 117:1757-71. 2004
    ..Here, we analyse how the kinetochore localization of the Drosophila spindle checkpoint proteins Bub1, Mad2, Bub3 and BubR1, behave in response to alterations in microtubule binding or tension...
  46. pmc Phosphorylation and activation of Bub1 on unattached chromosomes facilitate the spindle checkpoint
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
    EMBO J 23:3113-21. 2004
    ..The protein kinase Bub1 is an essential checkpoint component that resides at kinetochores during mitosis...
  47. pmc Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen
    Sergei Boichuk
    University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    J Virol 84:8007-20. 2010
    ..the hallmarks of a cellular DNA damage response (DDR), such as focal accumulation of gamma-H2AX and 53BP1, via Bub1 binding. Here we expand our characterization of LT effects on the DDR...
  48. pmc Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC
    G K Chan
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 146:941-54. 1999
    Human cells express two kinases that are related to the yeast mitotic checkpoint kinase BUB1. hBUB1 and hBUBR1 bind to kinetochores where they are postulated to be components of the mitotic checkpoint that monitors kinetochore activities ..
  49. ncbi Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression
    Erwan Watrin
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 16:863-74. 2006
    ..Mutation of Scc2 orthologs causes defects in cohesion, transcription, and development, resulting in Cornelia de Lange syndrome in humans...
  50. pmc Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction
    Shigeaki Saitoh
    Division of Cell Biology, Institute of Life Science, Kurume University, Kurume, Fukuoka 839 0864, Japan
    Mol Biol Cell 19:3885-97. 2008
    ..in fission yeast is regulated by two partially overlapping but distinct pathways: the Dam1/DASH and the Bub1 pathways. We show that Mad2 is localized on "unattached" as well as "tensionless" kinetochores...
  51. pmc BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1
    Rey Huei Chen
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 158:487-96. 2002
    ..Unattached kinetochores associate with several checkpoint proteins, including BubR1, Bub1, Bub3, Mad1, Mad2, and CENP-E...
  52. ncbi The multiple roles of Bub1 in chromosome segregation during mitosis and meiosis
    Francesco Marchetti
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
    Cell Cycle 9:58-63. 2010
    ..Among the SAC proteins, the budding uninhibited by benzimidazole protein 1 (Bub1), is a highly conserved protein of prominent importance for the proper functioning of the SAC...
  53. pmc Loading of the 3F3/2 antigen onto kinetochores is dependent on the ordered assembly of the spindle checkpoint proteins
    Oi Kwan Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Mol Biol Cell 17:4390-9. 2006
    ..attachment or of tension across sister kinetochores through phosphorylation of kinetochore proteins by the Mps1, Bub1, BubR1, Aurora B, and Plk1/Plx1 kinases...
  54. ncbi Somatic mutation of the hBUB1 mitotic checkpoint gene in primary lung cancer
    A Gemma
    Fourth Department of Internal Medicine, Nippon Medical School, Bunkyo ku, Tokyo, Japan
    Genes Chromosomes Cancer 29:213-8. 2000
    ..genomic DNA sequence of 30 human lung cancer cell lines and 30 primary lung cancer tumors for a mutation in the hBUB1 mitotic checkpoint gene...
  55. pmc Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1
    G K Chan
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 143:49-63. 1998
    ..screen to isolate interacting proteins that included the kinetochore proteins CENP-E, CENP-F, and hBUBR1, a BUB1-related kinase that was found to be mutated in some colorectal carcinomas (Cahill, D.P., C. Lengauer, J. Yu, G.J...
  56. pmc Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores
    R Fraschini
    Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy
    EMBO J 20:6648-59. 2001
    The kinetochore checkpoint pathway, involving the Mad1, Mad2, Mad3, Bub1, Bub3 and Mps1 proteins, prevents anaphase entry and mitotic exit by inhibiting the anaphase promoting complex activator Cdc20 in response to monopolar attachment of ..
  57. ncbi Mps1 phosphorylation by MAP kinase is required for kinetochore localization of spindle-checkpoint proteins
    Yong Zhao
    Graduate Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Curr Biol 16:1764-9. 2006
    ..activate the spindle checkpoint by recruiting several spindle-checkpoint proteins, including Mps1, Mad1, Mad2, Bub1, Bub3, and BubR1 (Mad3 in yeast)...
  58. pmc The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function
    Sheila Kadura
    Verna and Marrs McClean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Biol Cell 16:385-95. 2005
    ..to isolate SAC mutants in fission yeast yielded point mutations in three fission yeast SAC genes: mad1, bub3, and bub1. The bub1-A78V mutant is of particular interest because it produces a wild-type amount of protein that is mutated ..
  59. pmc Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles
    Shigeaki Saitoh
    Division of Cell Biology, Institute of Life Science, Kurume University, Fukuoka 839 0864, Japan
    Mol Biol Cell 16:3666-77. 2005
    ..During early mitosis, the spindle checkpoint proteins, such as Mad2 and Bub1, accumulate at kinetochores that do not associate with the spindle...
  60. ncbi Aurora B kinase-dependent recruitment of hZW10 and hROD to tensionless kinetochores
    Jakub K Famulski
    Department of Oncology, University of Alberta, Edmonton, Alberta T6G 1Z2, Canada
    Curr Biol 17:2143-9. 2007
    ..of tension, but not in the absence of kinetochore-microtubule attachments, results in the loss of hZW10, hROD, and hBub1 from kinetochores...
  61. ncbi Isolation and characterization of a Paramecium cDNA clone encoding a putative serine/threonine protein kinase
    Satoru Wada
    Graduate School of Life Sciences, Tohoku University, Aoba Aramaki, Sendai 980 8578, Japan
    Genetica 131:307-14. 2007
    ..Comparisons of expression level have shown that pcstk1 is expressed equally in cells at different stages (sexual and asexual). We discussed the possibility, as in other organisms, that a family of MAP kinase genes exists in P. caudatum...
  62. ncbi Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex
    Eulalie Buffin
    Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Curr Biol 15:856-61. 2005
    ..Moreover, Mad2, but not BubR1, Bub1, Bub3, or Mps1, requires Rod/Zw10 for its accumulation on unattached kinetochores...
  63. doi TRAIL inactivates the mitotic checkpoint and potentiates death induced by microtubule-targeting agents in human cancer cells
    Mijin Kim
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia Veterans Affairs Medical Center, Philadelphia, PA 19104, USA
    Cancer Res 68:3440-9. 2008
    ..of TRAIL to either nocodazole or paclitaxel (Taxol) reduced levels of the mitotic checkpoint proteins BubR1 and Bub1. BubR1 mutated for the caspase cleavage sites, but not wild-type BubR1, was resistant to cleavage induced by TRAIL ..
  64. doi Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content
    Jingyu Xu
    National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Canada
    Plant Biotechnol J 6:799-818. 2008
    ..Thus, alteration of this putative serine/threonine protein kinase site can be exploited to enhance DGAT1 activity, and expression of mutated DGAT1 can be used to enhance oil content...
  65. pmc Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores
    Song Tao Liu
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Mol Biol Cell 14:1638-51. 2003
    ..hMPS1 is required for MAD1, MAD2 but not hBUB1, hBUBR1 and hROD to bind to kinetochores...
  66. pmc Aurora-C kinase deficiency causes cytokinesis failure in meiosis I and production of large polyploid oocytes in mice
    Kuo Tai Yang
    Institute of Biomedical Sciences, Academia Sinica, Nankang, Taipei 115, Taiwan
    Mol Biol Cell 21:2371-83. 2010
    ..Furthermore, AurC-KD reduced Aurora-C and histone H3 phosphorylation and inhibited kinetochore localization of Bub1 and BubR1...
  67. pmc Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast
    K G Hardwick
    Department of Physiology, University of California, San Francisco 94143 0444, USA
    J Cell Biol 131:709-20. 1995
    ..assembly checkpoint mutants reveals that this response to microtubule-disrupting agents is defective in some (mad2, bub1, and bub3) but not all (mad3, bub2) mutant strains...
  68. ncbi Pericentromeric regions containing 1.688 satellite DNA sequences show anti-kinetochore antibody staining in prometaphase chromosomes of Drosophila melanogaster
    J P Abad
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Madrid, Spain
    Mol Gen Genet 264:371-7. 2000
    ..Immunostaining of prometaphase chromosomes with the kinetocore-specific anti-BUB1 antibody reveals the transient presence of this centromeric protein in all the regions containing the 1...
  69. ncbi Bub1 up-regulation and hyperphosphorylation promote malignant transformation in SV40 tag-induced transgenic mouse models
    Conghui Guo
    Department of Surgery, University of Western Ontario, London, Ontario, Canada
    Mol Cancer Res 4:957-69. 2006
    ..In particular, the spindle checkpoint component Bub1 was persistently up-regulated from early to advanced androgen-independent prostate cancer lesions...
  70. pmc Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoints
    D A Skoufias
    Institut de Biologie Structurale J P Ebel Commissariat à l Energie Atomique Centre National de la Recherche Scientifique, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Proc Natl Acad Sci U S A 98:4492-7. 2001
    ..A number of proteins, including mad2, bub1, and bubR1, have been implicated in the metaphase checkpoint control in mammalian cells...
  71. doi Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition
    Mukesh Jain
    Department of Plant Pathology, University of Florida, Gainesville, FL 32611 0680, USA
    Physiol Plant 134:161-73. 2008
    ..seed development. However, discordant in situ hybridization and immunolocalization data indicated that the expression of SbSnRK1b homologue in maize is under posttranscriptional control during endosperm development...
  72. pmc Novel interactors and a role for supervillin in early cytokinesis
    Tara C Smith
    Department of Cell Biology and Cell Dynamics Program, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Cytoskeleton (Hoboken) 67:346-64. 2010
    ..The interacting sequences from 12 of these proteins (BUB1, EPLIN/LIMA1, FLNA, HAX1, KIF14, KIFC3, MIF4GD/SLIP1, ODF2/Cenexin, RHAMM, STARD9/KIF16A, Tks5/SH3PXD2A, TNFAIP1) ..
  73. pmc Distinct chromosome segregation roles for spindle checkpoint proteins
    Cheryl D Warren
    McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 13:3029-41. 2002
    ..Loss of Bub1 or Bub3 protein elicits the largest effect. Analysis of Bub1p reveals the presence of two molecular functions...
  74. pmc The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes
    Chizuko Tsurumi
    Max Planck Institut fuer Immunbiologie, Developmental Biology, Freiburg, Germany
    J Cell Biol 167:1037-50. 2004
    In Xenopus oocytes, the spindle assembly checkpoint (SAC) kinase Bub1 is required for cytostatic factor (CSF)-induced metaphase arrest in meiosis II...
  75. pmc The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae
    S Bidlingmaier
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Cell Biol 21:2449-62. 2001
    ..Many Cbk1-related kinases have been shown to regulate cellular morphology...
  76. pmc Disruption of astral microtubule contact with the cell cortex activates a Bub1, Bub3, and Mad3-dependent checkpoint in fission yeast
    Sylvie Tournier
    Division of Yeast Genetics, National Institute for Medical Research, London NW7 1AA, United Kingdom
    Mol Biol Cell 15:3345-56. 2004
    ..By analyzing the timing of kinetochore separation, we find that this anaphase delay requires the Bub3, Mad3, and Bub1 but not the Mad1 or Mad2 spindle assembly checkpoint proteins...
  77. pmc DNA repair gene patterns as prognostic and predictive factors in molecular breast cancer subtypes
    Libero Santarpia
    Translational Research Unit, Department of Oncology, Istituto Toscano Tumori, Prato, Italy
    Oncologist 18:1063-73. 2013
    ..In ER-positive/HER2-negative tumors, the expression of nine genes (BUB1, FANCI, MNAT1, PARP2, PCNA, POLQ, RPA3, TOP2A, and UBE2V2) was associated with poor prognosis, and the expression ..
  78. ncbi Possible causes of chromosome instability: comparison of chromosomal abnormalities in cancer cell lines with mutations in BRCA1, BRCA2, CHK2 and BUB1
    M Grigorova
    Cancer Genomics Program, Department of Pathology, University of Cambridge, Hutchison MRC Research Centre, Cambridge, UK
    Cytogenet Genome Res 104:333-40. 2004
    ..However, two colorectal cancer lines with mutations in BUB1, a spindle checkpoint protein involved in chromosome segregation, had rather simple near-tetraploid karyotypes, ..
  79. ncbi Overexpression of the mitotic checkpoint genes BUB1, BUBR1, and BUB3 in gastric cancer--association with tumour cell proliferation
    Heike Grabsch
    Department of Histopathology, The Leeds Teaching Hospitals NHS Trust, Leeds, UK
    J Pathol 200:16-22. 2003
    ..To explore this possibility, the expression levels of BUB1, BUBR1, and BUB3 were determined in 43 gastric carcinomas and corresponding normal gastric mucosa by reverse ..
  80. pmc Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast
    Emily S Gillett
    Massachusetts Institute of Technology, Department of Biology, 77 Massachusetts Ave, 68 371 Cambridge, MA 02139, USA
    J Cell Biol 164:535-46. 2004
    ..include the mitotic arrest defective (MAD) genes MAD1-3, and the budding uninhibited by benzimidazole (BUB) genes BUB1 and BUB3...
  81. doi MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components
    Yuya Yamagishi
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113 0032, Japan
    Nat Cell Biol 14:746-52. 2012
    ..This phosphorylation promotes the in vitro binding to the Bub1-Bub3 complex, which is required for kinetochore-based SAC activation (Mad1-Mad2-Mad3 localization) and chromosome ..
  82. doi Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores
    Stephanie Heinrich
    Friedrich Miescher Laboratory of the Max Planck Society, Tuebingen, Germany
    J Cell Sci 125:4720-7. 2012
    ..for SAC protein localization to kinetochores and identify a three-layered hierarchy with Ark1 and Mph1 on top, Bub1 and Bub3 in the middle, and Mad3 as well as the Mad1-Mad2 complex at the lower end of the hierarchy...
  83. pmc MicroRNA-450a-3p represses cell proliferation and regulates embryo development by regulating Bub1 expression in mouse
    Min Luo
    Key Laboratory for Clinical Laboratory Diagnostics of Education Ministry of China, Chongqing Medical University, People s Republic of China
    PLoS ONE 7:e47914. 2012
    b>Bub1 is a critical component of the spindle assembly checkpoint (SAC) and closely linked to cell proliferation and differentiation...
  84. pmc Expression profiles of cohesins, shugoshins and spindle assembly checkpoint genes in rhesus macaque oocytes predict their susceptibility for aneuploidy during embryonic development
    Catherine Dupont
    Department of Physiology, School of Medicine, Wayne State University, Detroit, MI, USA
    Cell Cycle 11:740-8. 2012
    ..Statistically significant differences were observed between group 1 and group 2 for SGOL1 and BUB1. There were more oocytes with SGOL1 expression levels above the median in group 1, while oocytes lacking BUB1 were ..
  85. doi BUB1 immunolocalization in breast carcinoma: its nuclear localization as a potent prognostic factor of the patients
    Kiyoshi Takagi
    Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai, Miyagi ken, 980 8575, Japan
    Horm Cancer 4:92-102. 2013
    ..Results demonstrated that BUB1 expression level was closely correlated with the proliferation activity evaluated by Ki-67 labeling index (LI) of ..
  86. pmc BUB1 and BUBR1: multifaceted kinases of the cell cycle
    Victor M Bolanos-Garcia
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, England
    Trends Biochem Sci 36:141-50. 2011
    The multidomain protein kinases BUB1 and BUBR1 (Mad3 in yeast, worms and plants) are central components of the mitotic checkpoint for spindle assembly (SAC)...
  87. pmc Defining the molecular basis of BubR1 kinetochore interactions and APC/C-CDC20 inhibition
    Sheena D'Arcy
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 285:14764-76. 2010
    ..structure reveals divergence from the classical TPR fold and is highly similar to the TPR domain of budding yeast Bub1. Shared distinctive features include a disordered loop insertion, a 3(10)-helix, a tight turn involving glycine ..
  88. pmc BUB3 that dissociates from BUB1 activates caspase-independent mitotic death (CIMD)
    Y Niikura
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell Death Differ 17:1011-24. 2010
    ..cell death, termed caspase-independent mitotic death (CIMD), which is induced during early mitosis by partial BUB1 (a spindle checkpoint protein) depletion and defects in kinetochore-microtubule attachment...
  89. ncbi Identification and characterization of DBK, a novel putative serine/threonine protein kinase from human endothelial cells
    W Chu
    Department of Inflammation Autoimmune Diseases, Hoffmann La Roche Inc, Roche Research Center, Nutley NJ 07110 1199
    Eur J Biochem 225:695-702. 1994
    ..Using somatic cell hybrids, the DBK gene has been localized to human chromosome 14. The ubiquitous expression and high degree of conservation of DBK across species suggest that DBK may play an important role in cell functions...
  90. pmc Heterozygosity for a Bub1 mutation causes female-specific germ cell aneuploidy in mice
    Shawn Leland
    Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA
    Proc Natl Acad Sci U S A 106:12776-81. 2009
    ..Here, we report that heterozygosity for a mutation in the mitotic checkpoint kinase gene, Bub1, induces aneuploidy in female germ cells of mice and that the effect increases with advancing maternal age...
  91. pmc DNA damage activates the SAC in an ATM/ATR-dependent manner, independently of the kinetochore
    Eun Mi Kim
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, Virginia, United States of America
    PLoS Genet 4:e1000015. 2008
    ..The arrest requires the SAC proteins Mad1, Mad2, Mad3, Bub1, and Bub3 and works through Cdc20 and Pds1 but unlike the normal SAC, does not require a functional kinetochore...
  92. ncbi Cloning of the gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae
    Y Kakinuma
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Biochem Biophys Res Commun 216:985-92. 1995
    ..The results suggest that spermine uptake by this organism is probably regulated by phosphorylation and dephosphorylation...
  93. pmc Transcriptional signatures of Itk-deficient CD3+, CD4+ and CD8+ T-cells
    K Emelie M Blomberg
    Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 14186 Huddinge, Sweden
    BMC Genomics 10:233. 2009
    ..We have characterized the transcriptome of Itk-deficient CD3+ T-cells, including CD4+ and CD8+ subsets, using Affymetrix microarrays...
  94. pmc Gene expression time-series analysis of camptothecin effects in U87-MG and DBTRG-05 glioblastoma cell lines
    Elena Morandi
    Excellence Environmental Carcinogenesis, Lab, Mater, Environmental Protection and Health Prevention Agency, Emilia Romagna Region EPA, Bologna County, Italy
    Mol Cancer 7:66. 2008
    ..Here we report time-related changes and cell line specific patterns of gene expression after CPT treatment by using two p53 wild-type glioblastoma cell lines, U87-MG and DBTRG-05, with different sensitivities to TopoI inhibition...
  95. ncbi Red1p, a MEK1-dependent phosphoprotein that physically interacts with Hop1p during meiosis in yeast
    T de los Santos
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, SUNY Stony Brook, Stony Brook, New York 11794 5215, USA
    J Biol Chem 274:1783-90. 1999
    ..Thus, MEK1-dependent phosphorylation of Red1p facilitates the formation of Hop1p/Red1p hetero-oligomers, thereby enabling the formation of functional AEs...
  96. ncbi Knockdown of B-Raf impairs spindle formation and the mitotic checkpoint in human somatic cells
    M K Borysova
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA
    Cell Cycle 7:2894-901. 2008
    ..spindle assembly checkpoint was found to be dependent on B-Raf as evident by the inability of checkpoint proteins Bub1 and Mad2 to localize to unattached kinetochores in HeLa cells treated with B-Raf siRNA...
  97. doi Impaired Bub1 function in vivo compromises tension-dependent checkpoint function leading to aneuploidy and tumorigenesis
    Mark Schliekelman
    Curriculum in Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
    Cancer Res 69:45-54. 2009
    b>Bub1 is a serine/threonine kinase originally described as a core component of the spindle assembly checkpoint (SAC) mechanism in yeast...
  98. pmc Bub1-mediated adaptation of the spindle checkpoint
    Greicy H Goto
    Center for Childhood Cancer, The Research Institute at Nationwide Children s Hospital, Columbus, Ohio, United States of America
    PLoS Genet 7:e1001282. 2011
    ..Our results strongly suggest that Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for ..
  99. ncbi The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner
    J W Davenport
    Department of Virology and Molecular Biology, St Jude Children s Research Hospital, Memphis, Tennessee, 38105, USA
    Genomics 55:113-7. 1999
    ..Northern blots of normal tissues show that mouse Bub1a and Bub1b genes are expressed in thymus and spleen, but not in nondividing tissues...
  100. pmc Laterally attached kinetochores recruit the checkpoint protein Bub1, but satisfy the spindle checkpoint
    Michelle M Shimogawa
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Cell Cycle 9:3619-28. 2010
    ..DAM1-765 kinetochores recruit the spindle checkpoint protein Bub1, however Bub1 localization to DAM1-765 kinetochores is not sufficient to cause a cell cycle arrest...
  101. ncbi Overexpression of the mitotic spindle assembly checkpoint genes hBUB1, hBUBR1 and hMAD2 in thyroid carcinomas with aggressive nature
    Nobuyuki Wada
    Department of General Surgery, Yokohama City University, 3 9 Fukuura, Kanazawa Ku, Yokohama City, Kanagawa, Japan
    Anticancer Res 28:139-44. 2008
    ..MSAC gene expressions have been reported to be associated with tumor cell proliferation or unfavorable cancer behavior. The present study was conducted to preliminary investigate the MSAC gene expressions in thyroid neoplasms...

Research Grants83

  1. Spindle Assembly Checkpoint, Chromosomal Instability, and Cancer
    Pumin Zhang; Fiscal Year: 2010
    Aneuploidy is a hallmark of the vast majority of human solid tumors. Mutations in mitotic checkpoint genes such as BUB1, BUBR1 or MAD2 have been shown to be involved in generation of aneuploidy...
  2. Kinetochore Function and Cell Cycle Progression
    Katsumi Kitagawa; Fiscal Year: 2007
    ..The principal investigator has shown that the spindle checkpoint protein Bub1 binds to Skp1 and that Bub1 associates with CEN DNA via Skp1...
  3. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2009
    ..In Aim 2, we will investigate how Bub1 (a spindle checkpoint kinase) controls Sgo1 (Shugoshin, a tension sensor at the kinetochore)...
  4. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2010
    ..In Aim 2, we will investigate how Bub1 (a spindle checkpoint kinase) controls Sgo1 (Shugoshin, a tension sensor at the kinetochore)...
  5. Nuclear Transport Factors in Chromosomal Instability
    Jan van Deursen; Fiscal Year: 2007
    ..instability, mutations have been identified in genes that encode for mitotic checkpoint proteins, such as Bub1 BubR1 and Mad2...
  6. Regulation of the Spindle Checkpoint Protein Bub1p
    YANIRA GONZALEZ; Fiscal Year: 2007
    ..Interestingly, our lab has identified a mutation in the Mad3-like region (bub1-A78V) of the essential protein kinase Bub1p that disrupts the nuclear and kinetochore localization of Bub1p, Bub1p ..
  7. BUBRI in Normal and Neoplastic Growth
    Jan van Deursen; Fiscal Year: 2006
    ..A subset of these cancers is associated with mutations in genes encoding for mitotic checkpoint proteins such as BUB1, BUBR1 or MAD2...
  8. Mechanisms of Mitotic Activation of the ATM kinase
    Bo Xu; Fiscal Year: 2009
    ..depth studies have found that Aurora-B associated with ATM during mitosis and ATM was required for the activity of Bub1. Further we found that ATM phosphorylated Bub1 (at Ser314) and Mad 1(at Ser214)...
  9. CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTS
    PETER KARL SORGER; Fiscal Year: 2010
    ..and Bub proteins to kinetochores will be studied, with particular emphasis on the members of the Ndc80 complex (4) Bub1, a kinase with dual functions in checkpoint control and chromosome-microtubule attachment will be analyzed using ..
  10. Role of Bub1 in SV40 large T antigen mediated transformation and replication
    Ole Gjoerup; Fiscal Year: 2010
    ..We have identified an interaction of LT with the mitotic regulator Bub1. Bub1 is critical for preserving genome integrity...
  11. PTEN Deficiency and Tumor Development
    Yuxin Yin; Fiscal Year: 2010
    ..Of significant interest was our finding that the levels of Bub1 and aurora B which are two critical mitotic checkpoint regulators are reduced in Pten null cells...
  12. Mitotic Checkpoint Proteins in Tumorigenesis
    Frank McKeon; Fiscal Year: 2006
    ..abnormal chromosome segregation are killed in a manner dependent on signaling from two mitotic checkpoint proteins, BUB1 and MAD3L, to the tumor suppressor p53...
  13. BUBR1 in the Spindle Checkpoint and Tumor Suppression
    Wei Dai; Fiscal Year: 2006
    ..mechanism in human cancer development, the PI's laboratory has focused on the roles of two protein kinases, hBUB1 and hBUBR1 (42;53;81), mutations of which are detected in colorectal cancer cells (4)...
  14. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2009
    ..assisted conformational change of Mad2 in MCC assembly; (2) to determine the role of phosphorylation of Cdc20 by Bub1 in MCC assembly; and (3) to determine the role of Cdc20 autoubiquitination and degradation in MCC disassembly...
  15. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2010
    ..assisted conformational change of Mad2 in MCC assembly;(2) to determine the role of phosphorylation of Cdc20 by Bub1 in MCC assembly;and (3) to determine the role of Cdc20 autoubiquitination and degradation in MCC disassembly...
  16. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2009
    ..assisted conformational change of Mad2 in MCC assembly; (2) to determine the role of phosphorylation of Cdc20 by Bub1 in MCC assembly; and (3) to determine the role of Cdc20 autoubiquitination and degradation in MCC disassembly...
  17. Bub 1 in Chromosomal Instability and Tumorigenesis
    Jan van Deursen; Fiscal Year: 2007
    ..In certain cancers with aneuploidy, mutations have been identified in the mitotic checkpoint genes Bub1, BubR1 and Mad2. Epigenetic downregulation of Bub1 and BubR1 expression has also been observed in aneuploid tumors...
  18. Bub 1 in Chromosomal Instability and Tumorigenesis
    Jan M van Deursen; Fiscal Year: 2010
    ..In certain cancers with aneuploidy, mutations have been identified in the mitotic checkpoint genes Bub1, BubR1 and Mad2. Epigenetic downregulation of Bub1 and BubR1 expression has also been observed in aneuploid tumors...
  19. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2010
    ..of Plk1 /Aurora B in prophase? We have recently provided evidence to suggest that the spindle checkpoint kinase Bub1 targets and the Sgo1 centromeric protein and protein phosphatase 2A (PP2A) to centromeres where they counteract the ..
  20. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2007
    ..of Plk1 /Aurora B in prophase? We have recently provided evidence to suggest that the spindle checkpoint kinase Bub1 targets and the Sgo1 centromeric protein and protein phosphatase 2A (PP2A) to centromeres where they counteract the ..
  21. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2009
    ..of Plk1 /Aurora B in prophase? We have recently provided evidence to suggest that the spindle checkpoint kinase Bub1 targets and the Sgo1 centromeric protein and protein phosphatase 2A (PP2A) to centromeres where they counteract the ..
  22. Mechanisms of Mitotic Activation of the ATM kinase
    Bo Xu; Fiscal Year: 2010
    ..depth studies have found that Aurora-B associated with ATM during mitosis and ATM was required for the activity of Bub1. Further we found that ATM phosphorylated Bub1 (at Ser314) and Mad 1(at Ser214)...
  23. Functional Analysis of Mitotic Checkpoint Gene Bub1b
    Rakesh Goorha; Fiscal Year: 2005
    ..The murine homologue (Bub1b) of the yeast mitotic checkpoint protein Bub1 was discovered by the principal investigator and shown to be expressed in a cell cycle-specific manner...
  24. The role of hBUB1-p53 pathway in genomic stability.
    Sam Thiagalingam; Fiscal Year: 2007
    ..Unexpectedly, the second putative kinase isolated in the screen is hBUB1. BUB1 was originally isolated as a spindle assembly checkpoint gene in yeast and harbors mutations in a subset of ..
  25. Mitotic Checkpoint Regulators: Roles in Development and Cancer
    William F Marzluff; Fiscal Year: 2010
    ..Specifically, prompted by the identification of Bub1 mutations in certain colon cancer cell lines, in vitro studies have shown that interference with this mitotic ..
  26. Mitotic Checkpoint Regulators: Roles in Development and Cancer
    Terry van Dyke; Fiscal Year: 2009
    ..Specifically, prompted by the identification of Bub1 mutations in certain colon cancer cell lines, in vitro studies have shown that interference with this mitotic ..
  27. Mitotic Checkpoint Regulators: Roles in Development and Cancer
    Terry van Dyke; Fiscal Year: 2007
    ..Specifically, prompted by the identification of Bub1 mutations in certain colon cancer cell lines, in vitro studies have shown that interference with this mitotic ..
  28. Mitotic Checkpoint Regulators: Roles in Development and Cancer
    Terry van Dyke; Fiscal Year: 2006
    ..Specifically, prompted by the identification of Bub1 mutations in certain colon cancer cell lines, in vitro studies have shown that interference with this mitotic ..
  29. Identification of Anticancer Drug Targets
    Robert Wilson; Fiscal Year: 2004
    ..of genes that bring about synthetic lethality represent potential drug targets for cancers with mutations in hBUB1 or hMSH2...
  30. Chemical Approaches to Protein Phosphorylation
    Philip Cole; Fiscal Year: 2007
    ..kinase substrate recognition and regulation, including SR Ser/Thr kinases, Her2/neu tyrosine kinase, MAP kinases, Bub1 kinase, and protein kinase A...
  31. Chemical Approaches to Protein Phosphorylation
    Philip A Cole; Fiscal Year: 2010
    ..kinase substrate recognition and regulation, including SR Ser/Thr kinases, Her2/neu tyrosine kinase, MAP kinases, Bub1 kinase, and protein kinase A...