BTRC

Summary

Gene Symbol: BTRC
Description: beta-transducin repeat containing E3 ubiquitin protein ligase
Alias: BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP, E3RSIkappaB, F-box and WD repeats protein beta-TrCP, F-box and WD-repeat protein 1B, F-box/WD repeat-containing protein 1A, beta-TrCP1, epididymis tissue protein Li 2a, pIkappaBalpha-E3 receptor subunit
Species: human

Top Publications

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
  2. ncbi Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, CA, USA
    PLoS Pathog 5:e1000450. 2009
  3. ncbi Serine-threonine ubiquitination mediates downregulation of BST-2/tetherin and relief of restricted virion release by HIV-1 Vpu
    Andrey A Tokarev
    Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0679, USA
    J Virol 85:51-63. 2011
  4. ncbi Degradation of Mcl-1 by beta-TrCP mediates glycogen synthase kinase 3-induced tumor suppression and chemosensitization
    Qingqing Ding
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Mol Cell Biol 27:4006-17. 2007
  5. ncbi Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps
    Javier G Magadán
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1000869. 2010
  6. ncbi The ubiquitin ligase SCF(betaTrCP) regulates the degradation of the growth hormone receptor
    Peter van Kerkhof
    Department of Cell Biology, University Medical Center Utrecht and Institute of Biomembranes, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 282:20475-83. 2007
  7. ncbi Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP
    Yifeng Xia
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:2629-34. 2009
  8. ncbi β-TrCP is dispensable for Vpu's ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release
    Hanna Mari Tervo
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Retrovirology 8:9. 2011
  9. ncbi Silencing of both beta-TrCP1 and HOS (beta-TrCP2) is required to suppress human immunodeficiency virus type 1 Vpu-mediated CD4 down-modulation
    Christophe Butticaz
    Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland
    J Virol 81:1502-5. 2007
  10. ncbi Molecular dissection of the interactions among IkappaBalpha, FWD1, and Skp1 required for ubiquitin-mediated proteolysis of IkappaBalpha
    K Hattori
    Department of Molecular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 274:29641-7. 1999

Research Grants

  1. Regulation of the cell cycle by SCF and APC/C ubiquitin ligases
    Michele Pagano; Fiscal Year: 2009
  2. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2010
  3. Regulation of the G1 Phase by the Ubiquitin Pathway
    Michele Pagano; Fiscal Year: 2005
  4. CLOCK MECHANISM UNDERLYING DROSOPHILA RHYTHMIC BEHAVIOR
    Isaac Edery; Fiscal Year: 2007
  5. Gli Control of PTH-rP and Osteolysis in Breast Cancer
    Gregory Mundy; Fiscal Year: 2009
  6. Studies on ROC1-dependent Ubiquitin E3 Ligases
    Zhen Qiang Pan; Fiscal Year: 2006
  7. Regulation of PTHrP expression in chondrocytes by Gli2
    JULIE STERLING; Fiscal Year: 2005
  8. The molecular regulation of Gli2 in Hedgehog signaling
    Baolin Wang; Fiscal Year: 2007
  9. Genetic Analysis of Snail Superfamily Genes in Mice
    Thomas Gridley; Fiscal Year: 2009
  10. Genetic Analysis of Snail Superfamily Genes in Mice
    THOMAS H GRIDLEY; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications157 found, 100 shown here

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
    ..These results indicate that the long-sought IkappaBalpha-E3 is an SCF-like complex consisting of multiple proteins which are coordinately assembled during phosphorylation of IkappaBalpha in response to external signals...
  2. ncbi Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, CA, USA
    PLoS Pathog 5:e1000450. 2009
    ..Together, the data support a model in which Vpu co-opts the beta-TrCP/SCF E3 ubiquitin ligase complex to induce endosomal trafficking events that remove BST-2 from its site of action as a virion-tethering factor...
  3. ncbi Serine-threonine ubiquitination mediates downregulation of BST-2/tetherin and relief of restricted virion release by HIV-1 Vpu
    Andrey A Tokarev
    Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0679, USA
    J Virol 85:51-63. 2011
    ..Serine-threonine ubiquitination of BST-2 is likely part of the mechanism by which Vpu counteracts innate defenses...
  4. ncbi Degradation of Mcl-1 by beta-TrCP mediates glycogen synthase kinase 3-induced tumor suppression and chemosensitization
    Qingqing Ding
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Mol Cell Biol 27:4006-17. 2007
    ..Our results indicate that the turnover of Mcl-1 by beta-TrCP is an essential mechanism for GSK-3beta-induced apoptosis and contributes to GSK-3beta-mediated tumor suppression and chemosensitization...
  5. ncbi Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps
    Javier G Magadán
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1000869. 2010
    ..The multiple levels at which Vpu engages these cellular quality control mechanisms underscore the importance of ensuring profound suppression of CD4 to the life cycle of HIV-1...
  6. ncbi The ubiquitin ligase SCF(betaTrCP) regulates the degradation of the growth hormone receptor
    Peter van Kerkhof
    Department of Cell Biology, University Medical Center Utrecht and Institute of Biomembranes, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 282:20475-83. 2007
    ..Together, these findings provide direct evidence for a key role of the SCF(TrCP) in the endocytosis and degradation of an important factor in growth, immunity, and life span regulation...
  7. ncbi Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP
    Yifeng Xia
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:2629-34. 2009
    ..Our results identify IKK2 and beta-TrCP1 as novel regulators of the p53 pathway and suggest that blocking of IKK2 and beta-TrCP1 could be a means of regulating p53 stability and thereby modulating its biological activity...
  8. ncbi β-TrCP is dispensable for Vpu's ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release
    Hanna Mari Tervo
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Retrovirology 8:9. 2011
    ..Recently, it has been suggested that β-TrCP is also critically involved in Vpu's ability to overcome the CD317-mediated virion release block...
  9. ncbi Silencing of both beta-TrCP1 and HOS (beta-TrCP2) is required to suppress human immunodeficiency virus type 1 Vpu-mediated CD4 down-modulation
    Christophe Butticaz
    Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland
    J Virol 81:1502-5. 2007
    ....
  10. ncbi Molecular dissection of the interactions among IkappaBalpha, FWD1, and Skp1 required for ubiquitin-mediated proteolysis of IkappaBalpha
    K Hattori
    Department of Molecular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 274:29641-7. 1999
    The SCF complex containing Skp1, Cul1, and the F-box protein FWD1 (the mouse homologue of Drosophila Slimb and Xenopus beta-TrCP) functions as the ubiquitin ligase for IkappaBalpha...
  11. ncbi An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of beta-catenin
    M Kitagawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    EMBO J 18:2401-10. 1999
    ..Here we show that FWD1 (the mouse homologue of Slimb/betaTrCP), an F-box/WD40-repeat protein, specifically formed a multi-molecular complex with beta-catenin, Axin, GSK-3beta ..
  12. ncbi Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress
    Niels Mailand
    Institute of Cancer Biology and Centre for Genotoxic Stress Research, Danish Cancer Society, Strandboulevarden 49, DK 2100, Copenhagen, Denmark
    Mol Cell 23:307-18. 2006
    ..interaction was phosphorylation dependent and required the activity of the Plk1 kinase and the integrity of a betaTrCP recognition motif (phosphodegron) in the N terminus of Claspin...
  13. ncbi Constitutive and UV-induced fibronectin degradation is a ubiquitination-dependent process controlled by beta-TrCP
    Dipankar Ray
    Department of Molecular Pharmacology and Biological Chemistry, Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 281:23060-5. 2006
    ..Taken together, constitutive FN degradation, as well as UV-induced degradation, is ubiquitination dependent and controlled by beta-TrCP...
  14. ncbi Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progression
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:65-78. 2008
    ..We conclude that tight regulation of the Bora protein by its synthesis and degradation is critical for cell cycle progression...
  15. ncbi Site-specific ubiquitination exposes a linear motif to promote interferon-alpha receptor endocytosis
    K G Suresh Kumar
    Department of Animal Biology and 2Mari Lowe Center for Comparative Oncology Research, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 179:935-50. 2007
    ..The SCF(betaTrcp) (Skp1-Cullin1-F-box complex) E3 ubiquitin ligase that mediates IFNAR1 ubiquitination and degradation in cells ..
  16. ncbi SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein
    Takahiro Shirogane
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:26863-72. 2005
    ....
  17. ncbi NMR studies for identifying phosphopeptide ligands of the HIV-1 protein Vpu binding to the F-box protein beta-TrCP
    Nathalie Evrard-Todeschi
    Universite Rene Descartes Paris V, Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques UMR 8601 CNRS, 45 rue des Saint Peres, 75270 Paris Cedex 06, France
    Peptides 27:194-210. 2006
    ..These findings are in good agreement with a recently published X-ray structure of a shorter beta-Catenin fragment with the beta-TrCP complex...
  18. ncbi Phosphorylation-dependent regulation of stability and transforming potential of ETS transcriptional factor ESE-1 by p21-activated kinase 1
    Bramanandam Manavathi
    Department of Molecular and Cellular Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:19820-30. 2007
    ..These findings provide novel insights into the mechanism of transformation potential of ESE-1 and discovered that ESE-1 functions are coordinately regulated by Pak1 phosphorylation and beta-TrCP-dependent ubiquitin-proteasome pathways...
  19. ncbi Overexpression of human beta TrCP1 deleted of its F box induces tumorigenesis in transgenic mice
    Nadia Belaïdouni
    Department of Infectious Diseases, Department of INSERM U567, Institut Cochin, CNRS UMR8104, Université R Descartes Paris V, 27 rue du Faubourg Saint Jacques, 75014 Paris, France
    Oncogene 24:2271-6. 2005
    ..the synthesis of unphosphorylated beta-catenin that escapes recognition by the beta transducin repeat protein (beta TrCP1), the receptor of an ubiquitin...
  20. ncbi Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
    Geng Wu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:1445-56. 2003
    ....
  21. ncbi SAG/ROC-SCF beta-TrCP E3 ubiquitin ligase promotes pro-caspase-3 degradation as a mechanism of apoptosis protection
    Mingjia Tan
    Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109 0936, USA
    Neoplasia 8:1042-54. 2006
    ..Thus, pro-caspase-3 appears to be a substrate of SAG/ROC-SCF(beta-TrCP) E3 Ub ligase, which protects cells from apoptosis through increased apoptosis threshold by reducing the basal level of pro-caspase-3...
  22. ncbi High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML
    Manuel Favre
    Division of Infectious Diseases and Institute of Microbiology, University Hospital of Lausanne, Switzerland
    J Acquir Immune Defic Syndr 34:127-33. 2003
    ..6 human genes were investigated-tumor suppressor gene 101 (TSG101), beta-transducin repeats containing protein (betaTrCP), peptidyl-proly cis-trans isomerase, cyclophilin A (PPIA), integrase interactor 1 protein (INI1), Nef-associated ..
  23. ncbi beta-TrCP binding and processing of NF-kappaB2/p100 involve its phosphorylation at serines 866 and 870
    Chunyang Liang
    Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey Medical Center, P O Box 850, Hershey, Pennsylvania 17033, USA
    Cell Signal 18:1309-17. 2006
    ..These data suggest that p100 processing involves its phosphorylation at specific terminal serines, which form a binding site for beta-TrCP thereby regulating p100 ubiquitination...
  24. ncbi HIV-1 Vpu sequesters beta-transducin repeat-containing protein (betaTrCP) in the cytoplasm and provokes the accumulation of beta-catenin and other SCFbetaTrCP substrates
    Corinne Besnard-Guerin
    Institut Cochin, Department of Infectious Diseases, INSERM U567, CNRS UMR 8104, Université R Descartes Paris V, 27 rue du Faubourg St Jacques, 75014 Paris, France
    J Biol Chem 279:788-95. 2004
    ..adaptor for the proteasomal degradation of CD4 by recruiting CD4 and beta-transducin repeat-containing protein (betaTrCP), the receptor component of the multisubunit SCF-betaTrCP E3 ubiquitin ligase complex...
  25. ncbi Gli2 is targeted for ubiquitination and degradation by beta-TrCP ubiquitin ligase
    Neehar Bhatia
    Department of Dermatology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 281:19320-6. 2006
    ..These data identify beta-TrCP2 as a pivotal regulator of Gli2 expression and point to an important role for posttranslational modulation of GLI2 protein levels in Hh pathway-associated human prostate cancer...
  26. ncbi Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1
    David V Hansen
    Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
    Mol Biol Cell 15:5623-34. 2004
    ..In prophase, phosphorylation of Emi1 generates a D-pS-G-X-X-pS degron to recruit the SCF(betaTrCP) ubiquitin ligase, causing Emi1 destruction and allowing progression beyond prometaphase, but the kinases ..
  27. ncbi Role of F-box protein betaTrcp1 in mammary gland development and tumorigenesis
    Yasusei Kudo
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, NY 10016, USA
    Mol Cell Biol 24:8184-94. 2004
    ....
  28. ncbi SCFbeta-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase
    Jianping Jin
    Department of Pathology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 17:3062-74. 2003
    ..These data indicate that Cdc25A turnover is more complex than previously appreciated and suggest roles for an additional kinase(s) in Chk1-dependent Cdc25A turnover...
  29. ncbi M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:4419-24. 2004
    ..These results also establish the existence of a feedback loop between Cdc2 and Wee1A in somatic cells that depends on ubiquitination and protein degradation and ensures the rapid activation of Cdc2 when cells are ready to divide...
  30. ncbi S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth
    N Valerio Dorrello
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, NY 10016, USA
    Science 314:467-71. 2006
    ..rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF(betaTRCP)...
  31. ncbi Pseudosubstrate regulation of the SCF(beta-TrCP) ubiquitin ligase by hnRNP-U
    Matti Davis
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Genes Dev 16:439-51. 2002
    ..Our study points to a novel regulatory mechanism, which secures the localization, stability, substrate binding threshold, and efficacy of a specific protein-ubiquitin ligase...
  32. ncbi Identification of the receptor component of the IkappaBalpha-ubiquitin ligase
    A Yaron
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    Nature 396:590-4. 1998
    ..This component, which we denote E3RSIkappaB (pIkappaBalpha-E3 receptor subunit), binds specifically to pIkappaBalpha and promotes its in vitro ..
  33. ncbi The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro
    J T Winston
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 13:270-83. 1999
    ..Our results suggest that an SCFbeta-TRCP complex functions in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway...
  34. ncbi Signal-induced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP
    E Spencer
    Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9148 USA
    Genes Dev 13:284-94. 1999
    ..These biochemical and genetic data strongly suggest that Slimb/beta-TrCP is the specificity determinant for the signal-induced ubiquitination of IkappaBalpha...
  35. ncbi The F-box protein beta-TrCP associates with phosphorylated beta-catenin and regulates its activity in the cell
    M Hart
    Onyx Pharmaceuticals 3031 Research Drive Richmond California 94806 USA
    Curr Biol 9:207-10. 1999
    ..In contrast, beta-TrCP2 did not associate with beta-catenin. We conclude that beta-TrCP is a component of an E3 ubiquitin ligase that is responsible for the targeted degradation of phosphorylated beta-catenin...
  36. ncbi Differential interaction of plakoglobin and beta-catenin with the ubiquitin-proteasome system
    E Sadot
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Oncogene 19:1992-2001. 2000
    ....
  37. ncbi beta-TrCP mediates the signal-induced ubiquitination of IkappaBbeta
    C Wu
    Section of Immunobiology, Department of Molecular Biophysics, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 274:29591-4. 1999
    ..Therefore, these results indicate that beta-TrCP plays a critical role in the activation of NF-kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta...
  38. ncbi A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
    F Margottin
    CJF 97 03 INSERM, Institut Cochin de Genetique Moleculaire, Universite Paris V, Faculté de Médecine Cochin, France
    Mol Cell 1:565-74. 1998
    ..Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary ..
  39. ncbi SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response
    Angelo Peschiaroli
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, MSB 599, New York, New York 10016, USA
    Mol Cell 23:319-29. 2006
    ..We found that during recovery from the DNA replication checkpoint response, Claspin is degraded in a betaTrCP-dependent manner...
  40. ncbi The human immunodeficiency virus type 1 Vpu protein inhibits NF-kappa B activation by interfering with beta TrCP-mediated degradation of Ikappa B
    S Bour
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 276:15920-8. 2001
    ..This process involves the recruitment of human betaTrCP (TrCP), a key member of the SkpI-Cdc53-F-box E3 ubiquitin ligase complex that specifically interacts with ..
  41. ncbi The human immunodeficiency virus type 1 accessory protein Vpu induces apoptosis by suppressing the nuclear factor kappaB-dependent expression of antiapoptotic factors
    H Akari
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 194:1299-311. 2001
    Human immunodeficiency virus (HIV) type 1 Vpu is an integral membrane protein with a unique affinity for betaTrCP (TrCP), a key member of the SkpI-Cullin-F-box E3 ubiquitin ligase complex that is involved in the regulated degradation of ..
  42. ncbi A ubiquitin ligase complex essential for the NF-kappaB, Wnt/Wingless, and Hedgehog signaling pathways
    T Maniatis
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA
    Genes Dev 13:505-10. 1999
  43. ncbi Involvement of the betaTrCP in the ubiquitination and stability of the HIV-1 Vpu protein
    Nadia Belaïdouni
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Biophys Res Commun 357:688-93. 2007
    ..This process requires the recruitment of human betaTrCP, a component of the Skp1-Cullin-F box (SCF) ubiquitin ligase complex, that interacts with phosphorylated Vpu ..
  44. ncbi Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha
    M A Read
    LeukoSite, Inc, Cambridge, Massachusetts 02139, USA
    Mol Cell Biol 20:2326-33. 2000
    ..a minute fraction of total cellular Cul-1 is modified by Nedd8, the Cul-1 associated with ectopically expressed betaTrCP was highly enriched for the Nedd8-conjugated form...
  45. ncbi Oxidative stress-induced ubiquitination of RCAN1 mediated by SCFbeta-TrCP ubiquitin ligase
    Sachie Asada
    Kishi Initiative Research Unit, Frontier Research System, 2 1 Hirosawa, Wako, Saitama, Japan
    Int J Mol Med 22:95-104. 2008
    ..These results suggest that H2O2 induces SCF beta-TrCP-mediated ubiquitination of RCAN1, leading to a decrease in the protein level of RCAN1...
  46. ncbi Chronic low frequency/low volume resistance training reduces pro-inflammatory cytokine protein levels and TLR4 mRNA in rat skeletal muscle
    Nelo Eidy Zanchi
    Laboratory of Applied Nutrition and Metabolism, Physical Education and Sport School, University of Sao Paulo, Av Prof Mello Moraes, 65, Sao Paulo, SP, 05508 900, Brazil
    Eur J Appl Physiol 109:1095-102. 2010
    ....
  47. ncbi Regulation of postsynaptic RapGAP SPAR by Polo-like kinase 2 and the SCFbeta-TRCP ubiquitin ligase in hippocampal neurons
    Xiaolu L Ang
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:29424-32. 2008
    ..Our results identify a specific E3 ubiquitin ligase that mediates degradation of a key postsynaptic regulator of synaptic morphology and function...
  48. ncbi Multiple isoforms of beta-TrCP display differential activities in the regulation of Wnt signaling
    Eunjeong Seo
    Department of Life Science, The University of Seoul, Seoul, 130 743, Republic of Korea
    Cell Signal 21:43-51. 2009
    ..Overall, our data suggest that isoforms of beta-TrCPs generated by alternative splicing may have different biological roles...
  49. ncbi Analysis of the role of COP9 Signalosome (CSN) subunits in K562; the first link between CSN and autophagy
    Claire Pearce
    College of Life and Environmental Sciences, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    BMC Cell Biol 10:31. 2009
    ..Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells...
  50. ncbi LPS impairs phospholipid synthesis by triggering beta-transducin repeat-containing protein (beta-TrCP)-mediated polyubiquitination and degradation of the surfactant enzyme acyl-CoA:lysophosphatidylcholine acyltransferase I (LPCAT1)
    Chunbin Zou
    Department of Medicine, Acute Lung Injury Center of Excellence, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 286:2719-27. 2011
    ..LPCAT1 is the first lipogenic substrate for β-TrCP, and the results suggest that modulation of the GSK-3β-SCFβ(TrCP) E3 ligase effector pathway might be a unique strategy to optimize dipalmitoylphosphatidylcholine levels in sepsis...
  51. ncbi Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome
    Yakir Moshe
    Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 101:7937-42. 2004
    ..Transfection with an small interfering RNA duplex directed against Plk1 caused the accumulation of Emi1 in mitotically arrested HeLa cells. It is suggested that phosphorylation of Emi1 by Plk1 is involved in its degradation in mitosis...
  52. ncbi Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor
    K G Suresh Kumar
    Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:46614-20. 2004
    ..We discuss the complexities and specifics of the ubiquitination and degradation of IFNAR1, which is a beta-Trcp substrate that undergoes degradation via a lysosomal pathway...
  53. ncbi Disruption of Sag/Rbx2/Roc2 induces radiosensitization by increasing ROS levels and blocking NF-kappaB activation in mouse embryonic stem cells
    Mingjia Tan
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Free Radic Biol Med 49:976-83. 2010
    ....
  54. ncbi Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor
    Sabyasachi Bhattacharya
    Department of Animal Biology and Mari Lowe Center for Comparative Oncology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 285:2318-25. 2010
    ..These events play an important role in regulating ubiquitination and degradation of IFNAR1 as well as the extent of Type I interferon signaling...
  55. ncbi Pin1 stabilizes Emi1 during G2 phase by preventing its association with SCF(betatrcp)
    Cyril Bernis
    Centre de Recherche de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    EMBO Rep 8:91-8. 2007
    ..Paradoxically, Emi1 is stable during G2 phase, when cyclin A/cdk, Plx1 and SCF(betatrcp) (SCF for Skp1-Cul1-Fbox protein)--which play a role in its degradation--are active...
  56. ncbi Negative regulation of prolactin receptor stability and signaling mediated by SCF(beta-TrCP) E3 ubiquitin ligase
    Ying Li
    Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 24:4038-48. 2004
    ..These data indicate that PRLR is a novel SCF(beta-TrCP) substrate and implicate beta-TrCP as an important negative regulator of PRL signaling and cellular responses to this hormone...
  57. ncbi Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats
    W Spevak
    Ernst Boehringer Institute, Vienna, Austria
    Mol Cell Biol 13:4953-66. 1993
    ..The second clone, beta TrCP (beta-transducin repeat-containing protein), encodes a protein of 518 amino acids that shows significant ..
  58. ncbi Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination
    Xiaojing Tang
    Centre for Systems Biology, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada M5G 1X5
    Cell 129:1165-76. 2007
    ..This spatial variability may accommodate diverse acceptor lysine geometries in both substrates and the elongating ubiquitin chain and thereby increase catalytic efficiency...
  59. ncbi SCF/{beta}-TrCP promotes glycogen synthase kinase 3-dependent degradation of the Nrf2 transcription factor in a Keap1-independent manner
    Patricia Rada
    Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, c Arturo Duperier 4, 28029 Madrid, Spain
    Mol Cell Biol 31:1121-33. 2011
    ..Our results show for the first time that Nrf2 is targeted by GSK-3 for SCF/β-TrCP-dependent degradation. We propose a "dual degradation" model to describe the regulation of Nrf2 under different pathophysiological conditions...
  60. ncbi Acetaldehyde induces matrix metalloproteinase-9 gene expression via nuclear factor-kappaB and activator protein 1 signaling pathways in human hepatocellular carcinoma cells: Association with the invasive potential
    Chien Yun Hsiang
    Department of Microbiology, China Medical University, Taichung 40402, Taiwan
    Toxicol Lett 171:78-86. 2007
    ..These results suggested that acetaldehyde might be a potential factor involved in the invasiveness of HCC in alcoholic patients...
  61. ncbi Energy restriction as an antitumor target of thiazolidinediones
    Shuo Wei
    Division of Medicinal Chemistry, College of Pharmacy, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 285:9780-91. 2010
    ....
  62. ncbi Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1)
    Petros Marangos
    Department of Physiology, University College London, London WC1E 6BT, England, UK
    J Cell Biol 176:65-75. 2007
    ..for cyclin B destruction and inactivation of MPF, is present at prophase I and undergoes Skp1-Cul1-F-box/betaTrCP-mediated destruction immediately after germinal vesicle breakdown (GVBD)...
  63. ncbi Rotavirus and reovirus modulation of the interferon response
    Barbara Sherry
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA
    J Interferon Cytokine Res 29:559-67. 2009
    ..In sum, members of the family Reoviridae have evolved a variety of mechanisms to subvert the host's innate protective response...
  64. ncbi Vpu Antagonizes BST-2-Mediated Restriction of HIV-1 Release via beta-TrCP and Endo-Lysosomal Trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS Pathog 5:e1000450. 2009
    ..Together, the data support a model in which Vpu co-opts the beta-TrCP/SCF E3 ubiquitin ligase complex to induce endosomal trafficking events that remove BST-2 from its site of action as a virion-tethering factor...
  65. ncbi Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity
    M Karin
    Department of Pharmacology, Laboratory of Gene Regulation and Signal Transduction University of California, San Diego, La Jolla, California 92093 0636, USA
    Annu Rev Immunol 18:621-63. 2000
    ....
  66. ncbi Control of mammalian circadian rhythm by CKIepsilon-regulated proteasome-mediated PER2 degradation
    Erik J Eide
    Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    Mol Cell Biol 25:2795-807. 2005
    ..Cell culture-based biochemical assays combined with measurement of cell-based rhythm complement genetic studies to elucidate basic mechanisms controlling the mammalian clock...
  67. ncbi Modulation of SCF beta-TrCP-dependent I kappaB alpha ubiquitination by hydrogen peroxide
    Sami Banerjee
    Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0012, USA
    J Biol Chem 285:2665-75. 2010
    ....
  68. ncbi Cyclin-dependent kinase (CDK) phosphorylation destabilizes somatic Wee1 via multiple pathways
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 102:11663-8. 2005
    ..Using a specific inhibitor of CK2, we showed that the phosphorylation-dependent degradation of Wee1A is important for the proper onset of mitosis...
  69. ncbi A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs
    Jeffrey J Tung
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 102:4318-23. 2005
    ..We show here that exogenous Emi1 is unstable in CSF-arrested Xenopus eggs and is destroyed by the SCF(betaTrCP) ubiquitin ligase, suggesting that endogenous Emi1, an apparent 44-kDa protein, requires a stabilizing factor...
  70. ncbi The role of [beta]-transducin repeat-containing protein ([beta]-TrCP) in the regulation of NF-[kappa]B in vascular smooth muscle cells
    Xiaohong Wang
    Lillehei Heart Institute, Division of Cardiology, Department of Medicine, University of Minnesota, Minneapolis 55455, USA
    Arterioscler Thromb Vasc Biol 24:85-90. 2004
    ..We hypothesized that the E3-ligase, beta-transducin repeat-containing protein 1 (beta-TrCP1), was a rate-determining mediator that regulates the ubiquitin-mediated degradation of IkappaBalpha (in VSMC)...
  71. ncbi Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear beta-catenin expression
    L B Koppert
    Department of Pathology, Josephine Nefkens Institute, Erasmus University Medical Center, Rotterdam, The Netherlands
    Br J Cancer 90:892-9. 2004
    ..These data indicate that nuclear beta-catenin expression is indicative for activated Wnt signalling and that neither AXIN1 gene mutations nor AXIN1 locus LOH are involved in Wnt pathway activation in GEJ adenocarcinomas...
  72. ncbi The extracellular signal-regulated kinase-mitogen-activated protein kinase pathway phosphorylates and targets Cdc25A for SCF beta-TrCP-dependent degradation for cell cycle arrest
    Michitaka Isoda
    Department of Biology, Kyushu University, Hakozaki, Fukuoka, Japan
    Mol Biol Cell 20:2186-95. 2009
    ..These results suggest that strong ERK activation can target Cdc25A for degradation in a manner similar to, but independent of, Chk1 for cell cycle arrest...
  73. ncbi Reduced axin protein expression is associated with a poor prognosis in patients with squamous cell carcinoma of esophagus
    Anna Fen Yau Li
    Department of Pathology, Taipei Veterans General Hospital, Taipei, Taiwan
    Ann Surg Oncol 16:2486-93. 2009
    ....
  74. ncbi Human papillomavirus 16 E7 oncoprotein attenuates DNA damage checkpoint control by increasing the proteolytic turnover of claspin
    Nicole Spardy
    Biochemistry and Molecular Genetics Graduate Program, University of Pittsburgh School of Medicine, PA, USA
    Cancer Res 69:7022-9. 2009
    ....
  75. ncbi The tobacco-specific carcinogen NNK induces DNA methyltransferase 1 accumulation and tumor suppressor gene hypermethylation in mice and lung cancer patients
    Ruo Kai Lin
    Department of Pharmacology, National Cheng Kung University, Tainan, Republic of China
    J Clin Invest 120:521-32. 2010
    ..synthase kinase 3beta (GSK3beta) phosphorylated DNMT1 to recruit beta-transducin repeat-containing protein (betaTrCP), resulting in DNMT1 degradation, and that NNK activated AKT, inhibiting GSK3beta function and thereby ..
  76. ncbi Ability of CK2beta to selectively regulate cellular protein kinases
    Birgitte B Olsen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark
    Mol Cell Biochem 316:115-26. 2008
    ....
  77. ncbi CDC-25.1 stability is regulated by distinct domains to restrict cell division during embryogenesis in C. elegans
    Michael Hebeisen
    Department of Biology, McGill University, Montreal, Quebec, H3A 1B1, Canada
    Development 135:1259-69. 2008
    ..1 phosphatase during early embryogenesis to control its stability and turnover, in order to ensure the timely divisions of intestinal cells and coordinate them with the formation of the developing gut...
  78. ncbi Phosphorylation and regulation of beta-catenin by casein kinase I epsilon
    Chie Sakanaka
    Chiron Corporation, Horton Street, Emeryville, CA 94608, USA
    J Biochem 132:697-703. 2002
    ..I propose that phosphorylation of Ser-45 by CKI epsilon plays an important role in regulating beta-catenin stability...
  79. ncbi Phosphorylation by double-time/CKIepsilon and CKIalpha targets cubitus interruptus for Slimb/beta-TRCP-mediated proteolytic processing
    Jianhang Jia
    Center for Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    Dev Cell 9:819-30. 2005
    ..We propose that phosphorylation of Ci by CKI creates multiple Slimb/beta-TRCP binding sites that act cooperatively to recruit SCF(Slimb/beta-TRCP)...
  80. ncbi [Transducin beta-TrCP expressed in developmental hair follicle of mice]
    Yong dai Shen
    State Key Laboratory of Oral Diseases, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China
    Sichuan Da Xue Xue Bao Yi Xue Ban 39:612-4. 2008
    ..To identify the expression of antagonist beta-TrCP protein in Sonic hedgehog signal transduction pathway and Wnt signal transduction pathway in hair follicle tissues...
  81. ncbi The phospho-occupancy of an atypical SLIMB-binding site on PERIOD that is phosphorylated by DOUBLETIME controls the pace of the clock
    Joanna C Chiu
    Rutgers University, Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854, USA
    Genes Dev 22:1758-72. 2008
    ..We also use mass spectrometry to map phosphorylation sites on PER, leading to the identification of a number of "phospho-clusters" that explain several of the classic per mutants...
  82. ncbi Adjuvant immunochemotherapy with protein-bound polysaccharide K for colon cancer in relation to oncogenic beta-catenin activation
    Kaname Yamashita
    Division of Translational and Clinical Oncology previously, Division of Diagnostic Molecular Oncology and Surgical Oncology, Cancer Research Institute, Kanazawa University, 13 1 Takara machi, Kanazawa, Japan
    Dis Colon Rectum 50:1169-81. 2007
    ..chemotherapy with 5-fluorouracil alone...
  83. ncbi Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction
    Y Honaker
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO, USA
    Oncogene 29:3324-34. 2010
    ..The priming of Cdc25A by at least three kinases (Chk1, GSK-3beta, CK1alpha), some of which also require priming, ensures diverse extra- and intracellular signals interface with Cdc25A to precisely control cell division...
  84. ncbi Mechanisms of ubiquitin-mediated, limited processing of the NF-kappaB1 precursor protein p105
    A Ciechanover
    Department of Biochemistry and the Rappaport Institute for Research in the Medical Sciences, The Bruce Rappaport Faculty of Medicine, Haifa, Israel
    Biochimie 83:341-9. 2001
    ....
  85. ncbi Regulation of NF-kappa B2 p100 processing by its cis-acting domain
    Guoliang Qing
    Department of Cell Biology and Neuroscience, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 280:18-27. 2005
    ..Taken together, these studies highlight the mechanism by which the different sequences within p100 work in concert to regulate its processing and shed light on the mechanisms of how p100 processing is tightly and delicately controlled...
  86. ncbi Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase
    Hiroyuki Inuzuka
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:147-59. 2010
    ..Our results provide insight into the signaling pathways controlling Mdm2 destruction and further suggest that compromised regulation of Mdm2 results in attenuated p53 activity, thereby facilitating tumor progression...
  87. ncbi Plk4 trans-autophosphorylation regulates centriole number by controlling betaTrCP-mediated degradation
    Gernot Guderian
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 123:2163-9. 2010
    ..Here, we show that human Plk4 is subject to betaTrCP-dependent proteasomal degradation, indicating that this pathway is conserved from Drosophila to human...
  88. ncbi Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses
    S I Matsuzawa
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 7:915-26. 2001
    ..Expression of Siah is induced by p53, revealing a way of linking genotoxic injury to destruction of beta-catenin, thus reducing activity of Tcf/LEF transcription factors and contributing to cell cycle arrest...
  89. ncbi ERK and the F-box protein betaTRCP target STAT1 for degradation
    Surinder M Soond
    Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom
    J Biol Chem 283:16077-83. 2008
    ..Here we show that activated STAT1 is degraded at the proteasome by a mechanism involving the F-box E3 ligase, SCF(betaTRCP). Active p42/p44 MAPK-ERK phosphorylates STAT1 on serine 727 and targets it for proteasomal degradation...
  90. ncbi HIV-1 encoded virus protein U (Vpu) solution structure of the 41-62 hydrophilic region containing the phosphorylated sites Ser52 and Ser56
    Gael Coadou
    Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques UMR 8601 CNRS, Universite Rene Descartes Paris V, 45 rue des Saint Peres, 75270 Paris, Cedex 06, France
    Int J Biol Macromol 30:23-40. 2002
    ..Differences in this molecular region 50-62 suggest that conformational changes of Vpu_P play an important role in Vpu_P-induced degradation of CD4 molecules...
  91. ncbi A small molecule ubiquitination inhibitor blocks NF-kappa B-dependent cytokine expression in cells and rats
    David C Swinney
    Inflammatory and Viral Diseases Unit, Roche Bioscience, Palo Alto, California 94304, USA
    J Biol Chem 277:23573-81. 2002
    ..Ro106-9920 was observed to inhibit an ubiquitination activity that does not require betaTRCP but associates with IkappaB(alpha) and will ubiquitinate IkappaB(alpha) S32E,S36E (IkappaB(alpha)(ee)) ..
  92. ncbi Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation
    Xiaohui Wen
    Genentech, South San Francisco, CA 94080, USA
    Mol Cell Biol 30:1910-22. 2010
    ..We also confirmed that phosphorylation and betaTrCP/Cul1 are required for endogenous Gli3 processing and that this is inhibited by Hh...
  93. ncbi Expression of the Hedgehog antagonists Rab23 and Slimb/betaTrCP during mouse tooth development
    Isabelle Miletich
    Department of Craniofacial Development, Dental Institute, King s College London, Floor 28, Guy s Hospital, London SE1 9RT, UK
    Arch Oral Biol 50:147-51. 2005
    ..We have examined the expression pattern of the putative antagonists Rab23 and Slimb/betaTrCP during early murine odontogenesis and find that these molecules are expressed in the developing tooth...
  94. ncbi The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation
    Maria Elena Fernandez-Sanchez
    Institut Pasteur, Immunoregulation Unit and CNRS URA1961, 75724 Paris, France
    J Biol Chem 285:17390-7. 2010
    ..UBC3 was targeted for ubiquitination by the cullin-RING ubiquitin ligase SCF(betaTrCP)...
  95. ncbi Inhibition of lipopolysaccharide-induced interleukin-1beta and tumor necrosis factor-alpha production by menthone through nuclear factor-kappaB signaling pathway in HaCat cells
    Hui Man Cheng
    Institute for Integration of Traditional Chinese and Western Medicine, China Medical University Hospital, Taiwan, R O C
    Chin J Physiol 51:160-6. 2008
    ..To sum up, this study has provided molecular evidence for menthone effect on the LPS-induced cytokine production, NF-kappaB activation, and the involvement of I-kappaB and beta-TrCP...
  96. ncbi Negative feedback loop in T cell activation through IkappaB kinase-induced phosphorylation and degradation of Bcl10
    Camille Lobry
    Unite de Signalisation Moleculaire et Activation Cellulaire, Unité de Recherche Associée 2582, Centre National de la Recherche Scientifique, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 104:908-13. 2007
    ..Mutants of Bcl10 in the IKK phosphorylation site are resistant to degradation, accumulate in the nucleus, and lead to an increase in IL-2 production after T cell antigen receptor stimulation...
  97. ncbi A complex containing betaTrCP recruits Cdc34 to catalyse ubiquitination of IkappaBalpha
    L Vuillard
    School of Biomedical Science, University of St Andrews, Fife, UK
    FEBS Lett 455:311-4. 1999
    ..Phosphorylation dependent polyubiquitination is carried out by a multiprotein complex containing betaTrCP, Skp1 and Cdc53 (Cull)...
  98. ncbi Development of Protacs to target cancer-promoting proteins for ubiquitination and degradation
    Kathleen M Sakamoto
    Division of Hematology Oncology, Mattel Children s Hospital at the University of California Los Angeles, 90095 1752, USA
    Mol Cell Proteomics 2:1350-8. 2003
    ..Future improvements to this technology may yield a general approach to treat a number of human diseases, including cancer...
  99. ncbi TAZ promotes PC2 degradation through a SCFbeta-Trcp E3 ligase complex
    Yu Tian
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 27:6383-95. 2007
    ..These results establish a common role of TAZ across vertebrate species in a protein degradation pathway regulated by phosphorylation and implicate deficiencies in this pathway in the development of PKD...
  100. ncbi Directed expression of the HIV-1 accessory protein Vpu in Drosophila fat-body cells inhibits Toll-dependent immune responses
    François Leulier
    Centre de Génétique Moléculaire Centre National de la Recherche Scientifique, Batiment 26, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    EMBO Rep 4:976-81. 2003
    ....
  101. ncbi Regulated degradation of the HIV-1 Vpu protein through a betaTrCP-independent pathway limits the release of viral particles
    Emilie Estrabaud
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    PLoS Pathog 3:e104. 2007
    ..Vpu binds CD4 and simultaneously recruits the betaTrCP subunit of the SCF(betaTrCP) ubiquitin ligase complex through its constitutively phosphorylated DS52GXXS56 motif...

Research Grants67

  1. Regulation of the cell cycle by SCF and APC/C ubiquitin ligases
    Michele Pagano; Fiscal Year: 2009
    ..Despite the large number of F-box proteins, only three human SCF ubiquitin ligases (containing the F-box proteins betaTrcp, Fbw7 and Skp2, respectively) have well-established substrates, many of which are involved in cell cycle control (..
  2. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2010
    ..A better understanding of circadian clocks will potentially lead to new therapeutic approaches for treating human diseases. ..
  3. Regulation of the G1 Phase by the Ubiquitin Pathway
    Michele Pagano; Fiscal Year: 2005
    ..Under Aim 3, the principal investigator will also clone and characterize three proteins that he has found associated specifically with Fb13 in vivo. ..
  4. CLOCK MECHANISM UNDERLYING DROSOPHILA RHYTHMIC BEHAVIOR
    Isaac Edery; Fiscal Year: 2007
    ..This could be particularly rewarding in the case of Slimb and its mammalian homolog beta-TrCP, which recognize a variety of phosphotargets and have important roles in development, apoptosis, inflammatory responses and cancer. ..
  5. Gli Control of PTH-rP and Osteolysis in Breast Cancer
    Gregory Mundy; Fiscal Year: 2009
    ..We propose therefore that the Gli family of transcriptional regulators represents an important molecular pathway that regulates PTH-rP expression in breast cancer cells, and subsequent breast cancer-mediated osteolysis. ..
  6. Studies on ROC1-dependent Ubiquitin E3 Ligases
    Zhen Qiang Pan; Fiscal Year: 2006
    ....
  7. Regulation of PTHrP expression in chondrocytes by Gli2
    JULIE STERLING; Fiscal Year: 2005
    ..abstract_text> ..
  8. The molecular regulation of Gli2 in Hedgehog signaling
    Baolin Wang; Fiscal Year: 2007
    ..In addition, it may reveal a novel mechanism by which Beta-TrCP regulates Gli2 degradation and possibly Gli3 processing. ..
  9. Genetic Analysis of Snail Superfamily Genes in Mice
    Thomas Gridley; Fiscal Year: 2009
    ..mice; 2) test the hypothesis that post- translational regulation of SNAI1 protein by the GSK3beta kinase and the betaTrcp ubiquitin ligase are essential for Snai1 function in vivo; 3) test the hypothesis that Snail family genes are ..
  10. Genetic Analysis of Snail Superfamily Genes in Mice
    THOMAS H GRIDLEY; Fiscal Year: 2010
    ..mice;2) test the hypothesis that post- translational regulation of SNAI1 protein by the GSK3beta kinase and the betaTrcp ubiquitin ligase are essential for Snai1 function in vivo;3) test the hypothesis that Snail family genes are ..
  11. Effect of Gli2 on BMP-2 gene expression & bone formation
    Ming Zhao; Fiscal Year: 2007
    ..Full characterization of the roles of Gli2 protein in the regulation of BMP-2 gene expression and skeletogenesis should lead to insights into the molecular mechanisms of bone anabolic regulation. ..
  12. Role of HOS in cell transformation and apoptosis
    SERGE FUCHS; Fiscal Year: 2007
    ..Finally, in Specific Aim 3 we will use biochemical, pharmacological and genetic approaches to identify and characterize IFNAR1 kinase(s) and its role in IFNAR1 ubiquitination and degradation in vitro and in cells. ..
  13. Role of HOS in cell transformation and apoptosis
    SERGE FUCHS; Fiscal Year: 2009
    ..Finally, in Specific Aim 3 we will use biochemical, pharmacological and genetic approaches to identify and characterize IFNAR1 kinase(s) and its role in IFNAR1 ubiquitination and degradation in vitro and in cells. ..
  14. SNAIL-Dependent Regulation of EMT in Cancer
    STEPHEN JOSEPH WEISS; Fiscal Year: 2010
    ..These studies should not only define the interlocking role of Wnt, beta-catenin/TCF and Snail in regulating EMT in cancer, but also identify new targets for therapeutic intervention. ..
  15. SNAIL-Dependent Regulation of EMT in Cancer
    Stephen Weiss; Fiscal Year: 2007
    ..These studies should not only define the interlocking role of Wnt, beta-catenin/TCF and Snail in regulating EMT in cancer, but also identify new targets for therapeutic intervention. ..
  16. Xom Proteolysis During Early Vertebrate Embryogenesis
    Zhenglun Zhu; Fiscal Year: 2006
    ..abstract_text> ..
  17. NF-kappaB in myeloma cell growth and survival in vivo
    Babatunde Oyajobi; Fiscal Year: 2007
    ..the finding that expression in myeloma cells, of a dominant-negative (deltaF) mutant of the E3 Ubiquitin ligase betaTrCP/FWD1 that regulates NF-kB signaling, significantly reduced tumor growth in mice in vivo...
  18. Ubiquitination and Degradation in Cancer Therapy
    Kathleen Sakamoto; Fiscal Year: 2005
    ..Development of this technology will lead to novel approaches to treat androgen dependent and -independent disease in hopes of improving the survival of men with prostate cancer. ..
  19. Role of PER phosphorylation in circadian biology
    JOANNA CHIU; Fiscal Year: 2006
    ..I will integrate in vitro assays, tissue culture based systems, and whole animal approaches. The use of Drosophila melanogaster as the model animal provides a powerful system for genetic manipulations. ..
  20. Stability of prolactin receptor and prolactin signaling
    Serge Y Fuchs; Fiscal Year: 2010
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..
  21. Stability of prolactin receptor and prolactin signaling
    SERGE FUCHS; Fiscal Year: 2007
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..
  22. Stability of prolactin receptor and prolactin signaling
    SERGE FUCHS; Fiscal Year: 2009
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..