BTRC

Summary

Gene Symbol: BTRC
Description: beta-transducin repeat containing E3 ubiquitin protein ligase
Alias: BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP, E3RSIkappaB, F-box and WD repeats protein beta-TrCP, F-box and WD-repeat protein 1B, F-box/WD repeat-containing protein 1A, beta-TrCP1, epididymis tissue protein Li 2a, pIkappaBalpha-E3 receptor subunit
Species: human

Top Publications

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
  2. pmc Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, CA, USA
    PLoS Pathog 5:e1000450. 2009
  3. ncbi Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
    Geng Wu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:1445-56. 2003
  4. doi HIV-1 accessory proteins--ensuring viral survival in a hostile environment
    Michael H Malim
    Department of Infectious Diseases, King s College London School of Medicine, 2nd Floor, Borough Wing, Guy s Hospital, London Bridge, London, SE1 9RT, UK
    Cell Host Microbe 3:388-98. 2008
  5. pmc Degradation of Mcl-1 by beta-TrCP mediates glycogen synthase kinase 3-induced tumor suppression and chemosensitization
    Qingqing Ding
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Mol Cell Biol 27:4006-17. 2007
  6. pmc Serine-threonine ubiquitination mediates downregulation of BST-2/tetherin and relief of restricted virion release by HIV-1 Vpu
    Andrey A Tokarev
    Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0679, USA
    J Virol 85:51-63. 2011
  7. doi An insertion/deletion polymorphism in the 3' untranslated region of beta-transducin repeat-containing protein (betaTrCP) is associated with susceptibility for hepatocellular carcinoma in Chinese
    Shougong Chen
    Department of Forensic Medicine, Medical College of Soochow University, Suzhou, 215123 Jiangsu, PR China
    Biochem Biophys Res Commun 391:552-6. 2010
  8. ncbi The human immunodeficiency virus type 1 Vpu protein inhibits NF-kappa B activation by interfering with beta TrCP-mediated degradation of Ikappa B
    S Bour
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 276:15920-8. 2001
  9. pmc Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps
    Javier G Magadán
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1000869. 2010
  10. ncbi SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein
    Takahiro Shirogane
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:26863-72. 2005

Scientific Experts

Detail Information

Publications180 found, 100 shown here

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
    ..These results indicate that the long-sought IkappaBalpha-E3 is an SCF-like complex consisting of multiple proteins which are coordinately assembled during phosphorylation of IkappaBalpha in response to external signals...
  2. pmc Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, CA, USA
    PLoS Pathog 5:e1000450. 2009
    ..Together, the data support a model in which Vpu co-opts the beta-TrCP/SCF E3 ubiquitin ligase complex to induce endosomal trafficking events that remove BST-2 from its site of action as a virion-tethering factor...
  3. ncbi Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
    Geng Wu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:1445-56. 2003
    ....
  4. doi HIV-1 accessory proteins--ensuring viral survival in a hostile environment
    Michael H Malim
    Department of Infectious Diseases, King s College London School of Medicine, 2nd Floor, Borough Wing, Guy s Hospital, London Bridge, London, SE1 9RT, UK
    Cell Host Microbe 3:388-98. 2008
    ..Broadly speaking, the HIV-1 accessory proteins modify the local environment within infected cells to ensure viral persistence, replication, dissemination, and transmission...
  5. pmc Degradation of Mcl-1 by beta-TrCP mediates glycogen synthase kinase 3-induced tumor suppression and chemosensitization
    Qingqing Ding
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Mol Cell Biol 27:4006-17. 2007
    ..Our results indicate that the turnover of Mcl-1 by beta-TrCP is an essential mechanism for GSK-3beta-induced apoptosis and contributes to GSK-3beta-mediated tumor suppression and chemosensitization...
  6. pmc Serine-threonine ubiquitination mediates downregulation of BST-2/tetherin and relief of restricted virion release by HIV-1 Vpu
    Andrey A Tokarev
    Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0679, USA
    J Virol 85:51-63. 2011
    ..Serine-threonine ubiquitination of BST-2 is likely part of the mechanism by which Vpu counteracts innate defenses...
  7. doi An insertion/deletion polymorphism in the 3' untranslated region of beta-transducin repeat-containing protein (betaTrCP) is associated with susceptibility for hepatocellular carcinoma in Chinese
    Shougong Chen
    Department of Forensic Medicine, Medical College of Soochow University, Suzhou, 215123 Jiangsu, PR China
    Biochem Biophys Res Commun 391:552-6. 2010
    ..As a positive regulator of NFkappaB signaling pathway, beta-transducin repeat-containing protein (betaTrCP) is overexpressed and oncogenic in epithelial cancers, suggesting a potential role of betaTrCP in HCC ..
  8. ncbi The human immunodeficiency virus type 1 Vpu protein inhibits NF-kappa B activation by interfering with beta TrCP-mediated degradation of Ikappa B
    S Bour
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 276:15920-8. 2001
    ..This process involves the recruitment of human betaTrCP (TrCP), a key member of the SkpI-Cdc53-F-box E3 ubiquitin ligase complex that specifically interacts with ..
  9. pmc Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps
    Javier G Magadán
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1000869. 2010
    ..The multiple levels at which Vpu engages these cellular quality control mechanisms underscore the importance of ensuring profound suppression of CD4 to the life cycle of HIV-1...
  10. ncbi SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein
    Takahiro Shirogane
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:26863-72. 2005
    ....
  11. pmc Role of F-box protein betaTrcp1 in mammary gland development and tumorigenesis
    Yasusei Kudo
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, NY 10016, USA
    Mol Cell Biol 24:8184-94. 2004
    ....
  12. pmc PEST motif serine and tyrosine phosphorylation controls vascular endothelial growth factor receptor 2 stability and downregulation
    Rosana D Meyer
    Department of Pathology, Boston University Medical Campus, 670 Albany St, Boston, MA 02118, USA
    Mol Cell Biol 31:2010-25. 2011
    ..The PEST domain acts as a dual modulator of VEGFR-2; the phosphorylation of S1188/S1191 controls ubiquitination and degradation via β-Trcp1, where the phosphorylation of Y1173 through PKA/p38 MAPK controls the stability of VEGFR-2...
  13. pmc A novel mechanism by which thiazolidinediones facilitate the proteasomal degradation of cyclin D1 in cancer cells
    Shuo Wei
    Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 283:26759-70. 2008
    ..Moreover, we obtained evidence that this beta-TrCP-dependent degradation takes part in controlling cyclin D1 turnover when cancer cells undergo glucose starvation, which endows physiological relevance to this novel mechanism...
  14. pmc Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase
    Hiroyuki Inuzuka
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:147-59. 2010
    ..Our results provide insight into the signaling pathways controlling Mdm2 destruction and further suggest that compromised regulation of Mdm2 results in attenuated p53 activity, thereby facilitating tumor progression...
  15. pmc The N-terminal phosphodegron targets TAZ/WWTR1 protein for SCFβ-TrCP-dependent degradation in response to phosphatidylinositol 3-kinase inhibition
    Wei Huang
    Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    J Biol Chem 287:26245-53. 2012
    ..This study provides a novel mechanism of TAZ regulation and suggests a role of TAZ in modulating tissue growth and tumor development in response to PI3K signaling...
  16. pmc Structural and functional characterization of Nrf2 degradation by the glycogen synthase kinase 3/β-TrCP axis
    Patricia Rada
    Departamento de Bioquímica e Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Centro de Investigación en Red sobre Enfermedades Neurodegenerativas, Madrid, Spain
    Mol Cell Biol 32:3486-99. 2012
    ..This study establishes the structural parameters of the interaction of Nrf2 with the GSK-3/β-TrCP axis and its functional relevance in the regulation of Nrf2 by the signaling pathways that impinge on GSK-3...
  17. pmc Vpu directs the degradation of the human immunodeficiency virus restriction factor BST-2/Tetherin via a {beta}TrCP-dependent mechanism
    Janet L Douglas
    Oregon Health and Science University, Vaccine and Gene Therapy Institute, Beaverton, 97006, USA
    J Virol 83:7931-47. 2009
    ..to act as an adapter linking CD4 with the ubiquitin-proteasome machinery via interaction with the F-box protein betaTrCP. To identify additional cellular betaTrCP-dependent Vpu targets, we performed quantitative proteomics analyses ..
  18. pmc β-TrCP is dispensable for Vpu's ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release
    Hanna Mari Tervo
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Retrovirology 8:9. 2011
    ..Recently, it has been suggested that β-TrCP is also critically involved in Vpu's ability to overcome the CD317-mediated virion release block...
  19. ncbi Differential interaction of plakoglobin and beta-catenin with the ubiquitin-proteasome system
    E Sadot
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Oncogene 19:1992-2001. 2000
    ....
  20. ncbi RASSF1C, an isoform of the tumor suppressor RASSF1A, promotes the accumulation of beta-catenin by interacting with betaTrCP
    Emilie Estrabaud
    Institut Cochin, Département Maladies Infectieuses Institut National de la Sante et de la Recherche Medicale U567, 27 rue du Faubourg Saint Jacques, Bâtiment G Roussy, 75014 Paris, France
    Cancer Res 67:1054-61. 2007
    ..Here, we show that RASSF1C, but not RASSF1A, interacts with betaTrCP. Binding of RASSF1C to betaTrCP involves serine 18 and serine 19 of the SS(18)GYXS(19) motif present in RASSF1C ..
  21. pmc The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro
    J T Winston
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 13:270-83. 1999
    ..Our results suggest that an SCFbeta-TRCP complex functions in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway...
  22. ncbi NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases
    Jidong Liu
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 10:1511-8. 2002
    ..We suggest that by restricting SKP1-CUL1 interaction, CAND1 regulated the assembly of productive SCF ubiquitin ligases, allowing a common CUL1-ROC core to be utilized by a large number of SKP1-F box-substrate subcomplexes...
  23. ncbi beta-Trcp mediates ubiquitination and degradation of the erythropoietin receptor and controls cell proliferation
    Laure Meyer
    Institut Cochin, Departement d Hematologie, Paris, France
    Blood 109:5215-22. 2007
    ....
  24. doi Oncogene-mediated inhibition of glycogen synthase kinase 3 beta impairs degradation of prolactin receptor
    Alexander Plotnikov
    Department of Animal Biology, University of Pennsylvania, Philadelphia, PA 19104 4539, USA
    Cancer Res 68:1354-61. 2008
    ..breast cancer due to decreased phosphorylation of residue Ser(349), which, when phosphorylated, recruits the beta Trcp E3 ubiquitin ligase and facilitates PRLr degradation...
  25. pmc Thiazolidinediones mimic glucose starvation in facilitating Sp1 degradation through the up-regulation of beta-transducin repeat-containing protein
    Shuo Wei
    Division of Medicinal Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA
    Mol Pharmacol 76:47-57. 2009
    ..The ability of OSU-CG12 to mimic glucose starvation to activate beta-TrCP-mediated Sp1 degradation has translational potential to foster novel strategies for cancer therapy...
  26. ncbi A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
    F Margottin
    CJF 97 03 INSERM, Institut Cochin de Genetique Moleculaire, Universite Paris V, Faculté de Médecine Cochin, France
    Mol Cell 1:565-74. 1998
    ..Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary ..
  27. ncbi Split-hand/split-foot malformation 3 (SHFM3) at 10q24, development of rapid diagnostic methods and gene expression from the region
    Robert Lyle
    Department of Genetic Medicine and Development, University of Geneva Medical School, 1 rue Michel Servet, 1211 Geneva, Switzerland
    Am J Med Genet A 140:1384-95. 2006
    ..FISH and quantitative PCR that SHFM3 is caused by a minimal 325 kb duplication containing only two genes (BTRC and POLL)...
  28. ncbi Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage
    Luca Busino
    European Institute of Oncology, 435 Via Ripamonti, 20141 Milan, Italy
    Nature 426:87-91. 2003
    ..Our results show that beta-TrCP has a crucial role in mediating the response to DNA damage through Cdc25A degradation...
  29. pmc HIV-1 Vpu neutralizes the antiviral factor Tetherin/BST-2 by binding it and directing its beta-TrCP2-dependent degradation
    Bastien Mangeat
    Department of Dermatology and Venereology, University Hospitals and Medical School of Geneva, University of Geneva, Switzerland
    PLoS Pathog 5:e1000574. 2009
    ..Identification of tetherin binding to Vpu provides a potential novel target for the development of drugs aimed at inhibiting HIV-1 replication...
  30. pmc ATF4 degradation relies on a phosphorylation-dependent interaction with the SCF(betaTrCP) ubiquitin ligase
    I Lassot
    INSERM Unite 529, Interactions Moléculaires Hôte pathogène, 75014 Paris, France
    Mol Cell Biol 21:2192-202. 2001
    ..Here we show that the F-box protein betaTrCP, the receptor component of the SCF E3 ubiquitin ligase responsible for IkappaBalpha and beta-catenin degradation, ..
  31. pmc Ligand-stimulated downregulation of the alpha interferon receptor: role of protein kinase D2
    Hui Zheng
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104 4539, USA
    Mol Cell Biol 31:710-20. 2011
    ..The mechanisms of the ligand-inducible elimination of IFNAR1 are discussed, along with the potential medical significance of this regulation...
  32. pmc Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1
    David V Hansen
    Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
    Mol Biol Cell 15:5623-34. 2004
    ..In prophase, phosphorylation of Emi1 generates a D-pS-G-X-X-pS degron to recruit the SCF(betaTrCP) ubiquitin ligase, causing Emi1 destruction and allowing progression beyond prometaphase, but the kinases ..
  33. pmc An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of beta-catenin
    M Kitagawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    EMBO J 18:2401-10. 1999
    ..Here we show that FWD1 (the mouse homologue of Slimb/betaTrCP), an F-box/WD40-repeat protein, specifically formed a multi-molecular complex with beta-catenin, Axin, GSK-3beta ..
  34. pmc Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP
    Yifeng Xia
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:2629-34. 2009
    ..Our results identify IKK2 and beta-TrCP1 as novel regulators of the p53 pathway and suggest that blocking of IKK2 and beta-TrCP1 could be a means of regulating p53 stability and thereby modulating its biological activity...
  35. ncbi Involvement of the betaTrCP in the ubiquitination and stability of the HIV-1 Vpu protein
    Nadia Belaïdouni
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Biophys Res Commun 357:688-93. 2007
    ..This process requires the recruitment of human betaTrCP, a component of the Skp1-Cullin-F box (SCF) ubiquitin ligase complex, that interacts with phosphorylated Vpu ..
  36. doi Translation inhibitor Pdcd4 is targeted for degradation during tumor promotion
    Tobias Schmid
    Laboratory of Cancer Prevention, National Cancer Institute, Frederick, MD 21702, USA
    Cancer Res 68:1254-60. 2008
    ..Thus, because endogenous Pdcd4 levels are limiting for tumorigenesis, inhibiting signaling to Pdcd4 degradation may prove a valid strategy for cancer prevention and intervention...
  37. pmc Beta-TrCP recognizes a previously undescribed nonphosphorylated destruction motif in Cdc25A and Cdc25B phosphatases
    Yoshinori Kanemori
    Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    Proc Natl Acad Sci U S A 102:6279-84. 2005
    ....
  38. pmc Transforming growth factor beta facilitates beta-TrCP-mediated degradation of Cdc25A in a Smad3-dependent manner
    Dipankar Ray
    Department of Biochemistry and Molecular Genetics, University of Illinois College of Medicine, Chicago, IL 60607, USA
    Mol Cell Biol 25:3338-47. 2005
    ..These data suggest that Smad3 plays a key role in the regulation of Cdc25A ubiquitination by SCFbeta-TrCP and that Cdc25A stabilization observed in various cancers could be associated with defects in the TGF-beta-Smad3 pathway...
  39. ncbi Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1
    Kiyotaka Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Japan
    Biochem Biophys Res Commun 303:1209-16. 2003
    ..These observations thus suggest that TIP120A may function as a negative regulator of the SCF complex by binding to nonneddylated Cul1 and thereby preventing assembly of this ubiquitin ligase...
  40. ncbi Molecular dissection of the interactions among IkappaBalpha, FWD1, and Skp1 required for ubiquitin-mediated proteolysis of IkappaBalpha
    K Hattori
    Department of Molecular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 274:29641-7. 1999
    The SCF complex containing Skp1, Cul1, and the F-box protein FWD1 (the mouse homologue of Drosophila Slimb and Xenopus beta-TrCP) functions as the ubiquitin ligase for IkappaBalpha...
  41. pmc Multisite protein kinase A and glycogen synthase kinase 3beta phosphorylation leads to Gli3 ubiquitination by SCFbetaTrCP
    Denis Tempé
    département génétique et développement, Institut Cochin, 24 rue du Faubourg St Jacques, 75014 Paris, France
    Mol Cell Biol 26:4316-26. 2006
    ..We identified multiple betaTrCP-binding sites related to the DSGX2-4S motif in Gli3, which are intertwined with PKA and GSK3beta sites, and ..
  42. pmc The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1
    M Furukawa
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Mol Cell Biol 20:8185-97. 2000
    ..This pathway may be commonly utilized for the assembly of other cullin ligases...
  43. pmc Silencing of both beta-TrCP1 and HOS (beta-TrCP2) is required to suppress human immunodeficiency virus type 1 Vpu-mediated CD4 down-modulation
    Christophe Butticaz
    Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland
    J Virol 81:1502-5. 2007
    ....
  44. ncbi Identification of substrates for F-box proteins
    Jianping Jin
    Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 399:287-309. 2005
    ..In addition, we describe approaches for the use of immobilized phosphopeptides to identify F-box proteins that recognize particular phosphodegrons...
  45. ncbi TIP120A associates with cullins and modulates ubiquitin ligase activity
    Kyoeng Woo Min
    Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul 120 749, Korea
    J Biol Chem 278:15905-10. 2003
    ..These results suggest that TIP120A functions as a negative regulator of SCF E3 ubiquitin ligases and may modulate other cullin ligases in a similar fashion...
  46. ncbi The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin
    E Latres
    Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, NY 10016, USA
    Oncogene 18:849-54. 1999
    ..These results indicate that the Cul1/Skp1/beta-Trcp complex forms a ubiquitin ligase that mediates the degradation of beta-catenin...
  47. ncbi Stabilization of prolactin receptor in breast cancer cells
    Y Li
    Department of Animal Biology, University of Pennsylvania, Philadelphia, PA 19104 6046, USA
    Oncogene 25:1896-902. 2006
    ..These findings represent a novel mechanism through which altered PRLr stability may directly influence the pathogenesis of breast cancer...
  48. pmc Requirements for the selective degradation of CD4 receptor molecules by the human immunodeficiency virus type 1 Vpu protein in the endoplasmic reticulum
    Julie Binette
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, 110 avenue des Pins Ouest, Montreal, Quebec H2W 1R7, Canada
    Retrovirology 4:75. 2007
    ..Vpu is thought to act as an adaptor protein, connecting CD4 to the ubiquitin (Ub)-proteasome degradative system through an interaction with beta-TrCP, a component of the SCFbeta-TrCP E3 Ub ligase complex...
  49. ncbi The ubiquitin ligase SCF(betaTrCP) regulates the degradation of the growth hormone receptor
    Peter van Kerkhof
    Department of Cell Biology, University Medical Center Utrecht and Institute of Biomembranes, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 282:20475-83. 2007
    ..Together, these findings provide direct evidence for a key role of the SCF(TrCP) in the endocytosis and degradation of an important factor in growth, immunity, and life span regulation...
  50. ncbi A ubiquitin ligase complex essential for the NF-kappaB, Wnt/Wingless, and Hedgehog signaling pathways
    T Maniatis
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA
    Genes Dev 13:505-10. 1999
  51. pmc BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1
    Aurore Keutgens
    Interdisciplinary Cluster for Applied Genoproteomics, GIGA Research, Unit of Medical Chemistry, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Sart Tilman, 4000 Liege, Belgium
    J Biol Chem 285:25831-40. 2010
    ..Thus, our data defined a unique motif of BCL-3 that is needed for its recruitment to the proteasome and identified PSMB1 as a key protein required for the proteasome-mediated degradation of a nuclear and oncogenic IkappaB protein...
  52. ncbi Overexpression of human beta TrCP1 deleted of its F box induces tumorigenesis in transgenic mice
    Nadia Belaïdouni
    Department of Infectious Diseases, Department of INSERM U567, Institut Cochin, CNRS UMR8104, Université R Descartes Paris V, 27 rue du Faubourg Saint Jacques, 75014 Paris, France
    Oncogene 24:2271-6. 2005
    ..the synthesis of unphosphorylated beta-catenin that escapes recognition by the beta transducin repeat protein (beta TrCP1), the receptor of an ubiquitin...
  53. ncbi beta-TrCP binding and processing of NF-kappaB2/p100 involve its phosphorylation at serines 866 and 870
    Chunyang Liang
    Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey Medical Center, P O Box 850, Hershey, Pennsylvania 17033, USA
    Cell Signal 18:1309-17. 2006
    ..These data suggest that p100 processing involves its phosphorylation at specific terminal serines, which form a binding site for beta-TrCP thereby regulating p100 ubiquitination...
  54. ncbi SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response
    Angelo Peschiaroli
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, MSB 599, New York, New York 10016, USA
    Mol Cell 23:319-29. 2006
    ..We found that during recovery from the DNA replication checkpoint response, Claspin is degraded in a betaTrCP-dependent manner...
  55. ncbi Gli2 is targeted for ubiquitination and degradation by beta-TrCP ubiquitin ligase
    Neehar Bhatia
    Department of Dermatology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 281:19320-6. 2006
    ..These data identify beta-TrCP2 as a pivotal regulator of Gli2 expression and point to an important role for posttranslational modulation of GLI2 protein levels in Hh pathway-associated human prostate cancer...
  56. ncbi Phosphorylation-dependent regulation of stability and transforming potential of ETS transcriptional factor ESE-1 by p21-activated kinase 1
    Bramanandam Manavathi
    Department of Molecular and Cellular Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:19820-30. 2007
    ..These findings provide novel insights into the mechanism of transformation potential of ESE-1 and discovered that ESE-1 functions are coordinately regulated by Pak1 phosphorylation and beta-TrCP-dependent ubiquitin-proteasome pathways...
  57. ncbi Constitutive and UV-induced fibronectin degradation is a ubiquitination-dependent process controlled by beta-TrCP
    Dipankar Ray
    Department of Molecular Pharmacology and Biological Chemistry, Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 281:23060-5. 2006
    ..Taken together, constitutive FN degradation, as well as UV-induced degradation, is ubiquitination dependent and controlled by beta-TrCP...
  58. ncbi Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress
    Niels Mailand
    Institute of Cancer Biology and Centre for Genotoxic Stress Research, Danish Cancer Society, Strandboulevarden 49, DK 2100, Copenhagen, Denmark
    Mol Cell 23:307-18. 2006
    ..interaction was phosphorylation dependent and required the activity of the Plk1 kinase and the integrity of a betaTrCP recognition motif (phosphodegron) in the N terminus of Claspin...
  59. pmc SAG/ROC-SCF beta-TrCP E3 ubiquitin ligase promotes pro-caspase-3 degradation as a mechanism of apoptosis protection
    Mingjia Tan
    Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109 0936, USA
    Neoplasia 8:1042-54. 2006
    ..Thus, pro-caspase-3 appears to be a substrate of SAG/ROC-SCF(beta-TrCP) E3 Ub ligase, which protects cells from apoptosis through increased apoptosis threshold by reducing the basal level of pro-caspase-3...
  60. ncbi NMR studies for identifying phosphopeptide ligands of the HIV-1 protein Vpu binding to the F-box protein beta-TrCP
    Nathalie Evrard-Todeschi
    Universite Rene Descartes Paris V, Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques UMR 8601 CNRS, 45 rue des Saint Peres, 75270 Paris Cedex 06, France
    Peptides 27:194-210. 2006
    ..These findings are in good agreement with a recently published X-ray structure of a shorter beta-Catenin fragment with the beta-TrCP complex...
  61. ncbi Identification of the receptor component of the IkappaBalpha-ubiquitin ligase
    A Yaron
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    Nature 396:590-4. 1998
    ..This component, which we denote E3RSIkappaB (pIkappaBalpha-E3 receptor subunit), binds specifically to pIkappaBalpha and promotes its in vitro ..
  62. pmc Signal-induced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP
    E Spencer
    Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9148 USA
    Genes Dev 13:284-94. 1999
    ..These biochemical and genetic data strongly suggest that Slimb/beta-TrCP is the specificity determinant for the signal-induced ubiquitination of IkappaBalpha...
  63. ncbi The F-box protein beta-TrCP associates with phosphorylated beta-catenin and regulates its activity in the cell
    M Hart
    Onyx Pharmaceuticals 3031 Research Drive Richmond California 94806 USA
    Curr Biol 9:207-10. 1999
    ..In contrast, beta-TrCP2 did not associate with beta-catenin. We conclude that beta-TrCP is a component of an E3 ubiquitin ligase that is responsible for the targeted degradation of phosphorylated beta-catenin...
  64. ncbi beta-TrCP mediates the signal-induced ubiquitination of IkappaBbeta
    C Wu
    Section of Immunobiology, Department of Molecular Biophysics, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 274:29591-4. 1999
    ..Therefore, these results indicate that beta-TrCP plays a critical role in the activation of NF-kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta...
  65. ncbi S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth
    N Valerio Dorrello
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, NY 10016, USA
    Science 314:467-71. 2006
    ..rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF(betaTRCP)...
  66. pmc The human immunodeficiency virus type 1 accessory protein Vpu induces apoptosis by suppressing the nuclear factor kappaB-dependent expression of antiapoptotic factors
    H Akari
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 194:1299-311. 2001
    Human immunodeficiency virus (HIV) type 1 Vpu is an integral membrane protein with a unique affinity for betaTrCP (TrCP), a key member of the SkpI-Cullin-F-box E3 ubiquitin ligase complex that is involved in the regulated degradation of ..
  67. pmc Pseudosubstrate regulation of the SCF(beta-TrCP) ubiquitin ligase by hnRNP-U
    Matti Davis
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Genes Dev 16:439-51. 2002
    ..Our study points to a novel regulatory mechanism, which secures the localization, stability, substrate binding threshold, and efficacy of a specific protein-ubiquitin ligase...
  68. pmc SCFbeta-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase
    Jianping Jin
    Department of Pathology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 17:3062-74. 2003
    ..These data indicate that Cdc25A turnover is more complex than previously appreciated and suggest roles for an additional kinase(s) in Chk1-dependent Cdc25A turnover...
  69. pmc Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progression
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:65-78. 2008
    ..We conclude that tight regulation of the Bora protein by its synthesis and degradation is critical for cell cycle progression...
  70. ncbi High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML
    Manuel Favre
    Division of Infectious Diseases and Institute of Microbiology, University Hospital of Lausanne, Switzerland
    J Acquir Immune Defic Syndr 34:127-33. 2003
    ..6 human genes were investigated-tumor suppressor gene 101 (TSG101), beta-transducin repeats containing protein (betaTrCP), peptidyl-proly cis-trans isomerase, cyclophilin A (PPIA), integrase interactor 1 protein (INI1), Nef-associated ..
  71. ncbi HIV-1 Vpu sequesters beta-transducin repeat-containing protein (betaTrCP) in the cytoplasm and provokes the accumulation of beta-catenin and other SCFbetaTrCP substrates
    Corinne Besnard-Guerin
    Institut Cochin, Department of Infectious Diseases, INSERM U567, CNRS UMR 8104, Université R Descartes Paris V, 27 rue du Faubourg St Jacques, 75014 Paris, France
    J Biol Chem 279:788-95. 2004
    ..adaptor for the proteasomal degradation of CD4 by recruiting CD4 and beta-transducin repeat-containing protein (betaTrCP), the receptor component of the multisubunit SCF-betaTrCP E3 ubiquitin ligase complex...
  72. pmc M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:4419-24. 2004
    ..These results also establish the existence of a feedback loop between Cdc2 and Wee1A in somatic cells that depends on ubiquitination and protein degradation and ensures the rapid activation of Cdc2 when cells are ready to divide...
  73. pmc Site-specific ubiquitination exposes a linear motif to promote interferon-alpha receptor endocytosis
    K G Suresh Kumar
    Department of Animal Biology and 2Mari Lowe Center for Comparative Oncology Research, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 179:935-50. 2007
    ..The SCF(betaTrcp) (Skp1-Cullin1-F-box complex) E3 ubiquitin ligase that mediates IFNAR1 ubiquitination and degradation in cells ..
  74. ncbi Hierarchical order of phosphorylation events commits Cdc25A to betaTrCP-dependent degradation
    Maddalena Donzelli
    European Institute of Oncology, Milan, Italy
    Cell Cycle 3:469-71. 2004
    ..that regulation of Cdc25A protein abundance during S phase and in response to DNA damage is mediated by SCF(betaTrCP) activity...
  75. pmc Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha
    M A Read
    LeukoSite, Inc, Cambridge, Massachusetts 02139, USA
    Mol Cell Biol 20:2326-33. 2000
    ..a minute fraction of total cellular Cul-1 is modified by Nedd8, the Cul-1 associated with ectopically expressed betaTrCP was highly enriched for the Nedd8-conjugated form...
  76. ncbi Oxidative stress-induced ubiquitination of RCAN1 mediated by SCFbeta-TrCP ubiquitin ligase
    Sachie Asada
    Kishi Initiative Research Unit, Frontier Research System, 2 1 Hirosawa, Wako, Saitama, Japan
    Int J Mol Med 22:95-104. 2008
    ..These results suggest that H2O2 induces SCF beta-TrCP-mediated ubiquitination of RCAN1, leading to a decrease in the protein level of RCAN1...
  77. doi Chronic low frequency/low volume resistance training reduces pro-inflammatory cytokine protein levels and TLR4 mRNA in rat skeletal muscle
    Nelo Eidy Zanchi
    Laboratory of Applied Nutrition and Metabolism, Physical Education and Sport School, University of Sao Paulo, Av Prof Mello Moraes, 65, Sao Paulo, SP, 05508 900, Brazil
    Eur J Appl Physiol 109:1095-102. 2010
    ....
  78. pmc Regulation of postsynaptic RapGAP SPAR by Polo-like kinase 2 and the SCFbeta-TRCP ubiquitin ligase in hippocampal neurons
    Xiaolu L Ang
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:29424-32. 2008
    ..Our results identify a specific E3 ubiquitin ligase that mediates degradation of a key postsynaptic regulator of synaptic morphology and function...
  79. pmc Multiple isoforms of beta-TrCP display differential activities in the regulation of Wnt signaling
    Eunjeong Seo
    Department of Life Science, The University of Seoul, Seoul, 130 743, Republic of Korea
    Cell Signal 21:43-51. 2009
    ..Overall, our data suggest that isoforms of beta-TrCPs generated by alternative splicing may have different biological roles...
  80. pmc Analysis of the role of COP9 Signalosome (CSN) subunits in K562; the first link between CSN and autophagy
    Claire Pearce
    College of Life and Environmental Sciences, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    BMC Cell Biol 10:31. 2009
    ..Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells...
  81. pmc LPS impairs phospholipid synthesis by triggering beta-transducin repeat-containing protein (beta-TrCP)-mediated polyubiquitination and degradation of the surfactant enzyme acyl-CoA:lysophosphatidylcholine acyltransferase I (LPCAT1)
    Chunbin Zou
    Department of Medicine, Acute Lung Injury Center of Excellence, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 286:2719-27. 2011
    ..LPCAT1 is the first lipogenic substrate for β-TrCP, and the results suggest that modulation of the GSK-3β-SCFβ(TrCP) E3 ligase effector pathway might be a unique strategy to optimize dipalmitoylphosphatidylcholine levels in sepsis...
  82. pmc Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome
    Yakir Moshe
    Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 101:7937-42. 2004
    ..Transfection with an small interfering RNA duplex directed against Plk1 caused the accumulation of Emi1 in mitotically arrested HeLa cells. It is suggested that phosphorylation of Emi1 by Plk1 is involved in its degradation in mitosis...
  83. ncbi Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor
    K G Suresh Kumar
    Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:46614-20. 2004
    ..We discuss the complexities and specifics of the ubiquitination and degradation of IFNAR1, which is a beta-Trcp substrate that undergoes degradation via a lysosomal pathway...
  84. pmc Disruption of Sag/Rbx2/Roc2 induces radiosensitization by increasing ROS levels and blocking NF-kappaB activation in mouse embryonic stem cells
    Mingjia Tan
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Free Radic Biol Med 49:976-83. 2010
    ....
  85. pmc Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor
    Sabyasachi Bhattacharya
    Department of Animal Biology and Mari Lowe Center for Comparative Oncology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 285:2318-25. 2010
    ..These events play an important role in regulating ubiquitination and degradation of IFNAR1 as well as the extent of Type I interferon signaling...
  86. pmc Pin1 stabilizes Emi1 during G2 phase by preventing its association with SCF(betatrcp)
    Cyril Bernis
    Centre de Recherche de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    EMBO Rep 8:91-8. 2007
    ..Paradoxically, Emi1 is stable during G2 phase, when cyclin A/cdk, Plx1 and SCF(betatrcp) (SCF for Skp1-Cul1-Fbox protein)--which play a role in its degradation--are active...
  87. pmc SCF/{beta}-TrCP promotes glycogen synthase kinase 3-dependent degradation of the Nrf2 transcription factor in a Keap1-independent manner
    Patricia Rada
    Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, c Arturo Duperier 4, 28029 Madrid, Spain
    Mol Cell Biol 31:1121-33. 2011
    ..Our results show for the first time that Nrf2 is targeted by GSK-3 for SCF/β-TrCP-dependent degradation. We propose a "dual degradation" model to describe the regulation of Nrf2 under different pathophysiological conditions...
  88. pmc Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats
    W Spevak
    Ernst Boehringer Institute, Vienna, Austria
    Mol Cell Biol 13:4953-66. 1993
    ..The second clone, beta TrCP (beta-transducin repeat-containing protein), encodes a protein of 518 amino acids that shows significant ..
  89. ncbi Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination
    Xiaojing Tang
    Centre for Systems Biology, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada M5G 1X5
    Cell 129:1165-76. 2007
    ..This spatial variability may accommodate diverse acceptor lysine geometries in both substrates and the elongating ubiquitin chain and thereby increase catalytic efficiency...
  90. pmc Negative regulation of prolactin receptor stability and signaling mediated by SCF(beta-TrCP) E3 ubiquitin ligase
    Ying Li
    Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 24:4038-48. 2004
    ..These data indicate that PRLR is a novel SCF(beta-TrCP) substrate and implicate beta-TrCP as an important negative regulator of PRL signaling and cellular responses to this hormone...
  91. ncbi Acetaldehyde induces matrix metalloproteinase-9 gene expression via nuclear factor-kappaB and activator protein 1 signaling pathways in human hepatocellular carcinoma cells: Association with the invasive potential
    Chien Yun Hsiang
    Department of Microbiology, China Medical University, Taichung 40402, Taiwan
    Toxicol Lett 171:78-86. 2007
    ..These results suggested that acetaldehyde might be a potential factor involved in the invasiveness of HCC in alcoholic patients...
  92. pmc Energy restriction as an antitumor target of thiazolidinediones
    Shuo Wei
    Division of Medicinal Chemistry, College of Pharmacy, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 285:9780-91. 2010
    ....
  93. pmc Rotavirus and reovirus modulation of the interferon response
    Barbara Sherry
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA
    J Interferon Cytokine Res 29:559-67. 2009
    ..In sum, members of the family Reoviridae have evolved a variety of mechanisms to subvert the host's innate protective response...
  94. pmc Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1)
    Petros Marangos
    Department of Physiology, University College London, London WC1E 6BT, England, UK
    J Cell Biol 176:65-75. 2007
    ..for cyclin B destruction and inactivation of MPF, is present at prophase I and undergoes Skp1-Cul1-F-box/betaTrCP-mediated destruction immediately after germinal vesicle breakdown (GVBD)...
  95. ncbi Vpu Antagonizes BST-2-Mediated Restriction of HIV-1 Release via beta-TrCP and Endo-Lysosomal Trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS Pathog 5:e1000450. 2009
    ..Together, the data support a model in which Vpu co-opts the beta-TrCP/SCF E3 ubiquitin ligase complex to induce endosomal trafficking events that remove BST-2 from its site of action as a virion-tethering factor...
  96. ncbi Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity
    M Karin
    Department of Pharmacology, Laboratory of Gene Regulation and Signal Transduction University of California, San Diego, La Jolla, California 92093 0636, USA
    Annu Rev Immunol 18:621-63. 2000
    ....
  97. pmc A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs
    Jeffrey J Tung
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 102:4318-23. 2005
    ..We show here that exogenous Emi1 is unstable in CSF-arrested Xenopus eggs and is destroyed by the SCF(betaTrCP) ubiquitin ligase, suggesting that endogenous Emi1, an apparent 44-kDa protein, requires a stabilizing factor...
  98. ncbi The role of [beta]-transducin repeat-containing protein ([beta]-TrCP) in the regulation of NF-[kappa]B in vascular smooth muscle cells
    Xiaohong Wang
    Lillehei Heart Institute, Division of Cardiology, Department of Medicine, University of Minnesota, Minneapolis 55455, USA
    Arterioscler Thromb Vasc Biol 24:85-90. 2004
    ..We hypothesized that the E3-ligase, beta-transducin repeat-containing protein 1 (beta-TrCP1), was a rate-determining mediator that regulates the ubiquitin-mediated degradation of IkappaBalpha (in VSMC)...
  99. pmc Control of mammalian circadian rhythm by CKIepsilon-regulated proteasome-mediated PER2 degradation
    Erik J Eide
    Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    Mol Cell Biol 25:2795-807. 2005
    ..Cell culture-based biochemical assays combined with measurement of cell-based rhythm complement genetic studies to elucidate basic mechanisms controlling the mammalian clock...
  100. pmc Cyclin-dependent kinase (CDK) phosphorylation destabilizes somatic Wee1 via multiple pathways
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 102:11663-8. 2005
    ..Using a specific inhibitor of CK2, we showed that the phosphorylation-dependent degradation of Wee1A is important for the proper onset of mitosis...
  101. pmc Modulation of SCF beta-TrCP-dependent I kappaB alpha ubiquitination by hydrogen peroxide
    Sami Banerjee
    Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0012, USA
    J Biol Chem 285:2665-75. 2010
    ....

Research Grants67

  1. Regulation of the cell cycle by SCF and APC/C ubiquitin ligases
    Michele Pagano; Fiscal Year: 2009
    ..Despite the large number of F-box proteins, only three human SCF ubiquitin ligases (containing the F-box proteins betaTrcp, Fbw7 and Skp2, respectively) have well-established substrates, many of which are involved in cell cycle control (..
  2. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2010
    ..A better understanding of circadian clocks will potentially lead to new therapeutic approaches for treating human diseases. ..
  3. Regulation of the G1 Phase by the Ubiquitin Pathway
    Michele Pagano; Fiscal Year: 2005
    ..Under Aim 3, the principal investigator will also clone and characterize three proteins that he has found associated specifically with Fb13 in vivo. ..
  4. CLOCK MECHANISM UNDERLYING DROSOPHILA RHYTHMIC BEHAVIOR
    Isaac Edery; Fiscal Year: 2007
    ..This could be particularly rewarding in the case of Slimb and its mammalian homolog beta-TrCP, which recognize a variety of phosphotargets and have important roles in development, apoptosis, inflammatory responses and cancer. ..
  5. Gli Control of PTH-rP and Osteolysis in Breast Cancer
    Gregory Mundy; Fiscal Year: 2009
    ..We propose therefore that the Gli family of transcriptional regulators represents an important molecular pathway that regulates PTH-rP expression in breast cancer cells, and subsequent breast cancer-mediated osteolysis. ..
  6. Studies on ROC1-dependent Ubiquitin E3 Ligases
    Zhen Qiang Pan; Fiscal Year: 2006
    ....
  7. Regulation of PTHrP expression in chondrocytes by Gli2
    JULIE STERLING; Fiscal Year: 2005
    ..abstract_text> ..
  8. The molecular regulation of Gli2 in Hedgehog signaling
    Baolin Wang; Fiscal Year: 2007
    ..In addition, it may reveal a novel mechanism by which Beta-TrCP regulates Gli2 degradation and possibly Gli3 processing. ..
  9. Genetic Analysis of Snail Superfamily Genes in Mice
    THOMAS H GRIDLEY; Fiscal Year: 2010
    ..mice;2) test the hypothesis that post- translational regulation of SNAI1 protein by the GSK3beta kinase and the betaTrcp ubiquitin ligase are essential for Snai1 function in vivo;3) test the hypothesis that Snail family genes are ..
  10. Genetic Analysis of Snail Superfamily Genes in Mice
    Thomas Gridley; Fiscal Year: 2009
    ..mice; 2) test the hypothesis that post- translational regulation of SNAI1 protein by the GSK3beta kinase and the betaTrcp ubiquitin ligase are essential for Snai1 function in vivo; 3) test the hypothesis that Snail family genes are ..
  11. Ubiquitination and Degradation in Cancer Therapy
    Kathleen Sakamoto; Fiscal Year: 2005
    ..Development of this technology will lead to novel approaches to treat androgen dependent and -independent disease in hopes of improving the survival of men with prostate cancer. ..
  12. Effect of Gli2 on BMP-2 gene expression & bone formation
    Ming Zhao; Fiscal Year: 2007
    ..Full characterization of the roles of Gli2 protein in the regulation of BMP-2 gene expression and skeletogenesis should lead to insights into the molecular mechanisms of bone anabolic regulation. ..
  13. Role of HOS in cell transformation and apoptosis
    SERGE FUCHS; Fiscal Year: 2009
    ..Finally, in Specific Aim 3 we will use biochemical, pharmacological and genetic approaches to identify and characterize IFNAR1 kinase(s) and its role in IFNAR1 ubiquitination and degradation in vitro and in cells. ..
  14. Role of HOS in cell transformation and apoptosis
    SERGE FUCHS; Fiscal Year: 2007
    ..Finally, in Specific Aim 3 we will use biochemical, pharmacological and genetic approaches to identify and characterize IFNAR1 kinase(s) and its role in IFNAR1 ubiquitination and degradation in vitro and in cells. ..
  15. SNAIL-Dependent Regulation of EMT in Cancer
    Stephen Weiss; Fiscal Year: 2007
    ..These studies should not only define the interlocking role of Wnt, beta-catenin/TCF and Snail in regulating EMT in cancer, but also identify new targets for therapeutic intervention. ..
  16. SNAIL-Dependent Regulation of EMT in Cancer
    STEPHEN JOSEPH WEISS; Fiscal Year: 2010
    ..These studies should not only define the interlocking role of Wnt, beta-catenin/TCF and Snail in regulating EMT in cancer, but also identify new targets for therapeutic intervention. ..
  17. Xom Proteolysis During Early Vertebrate Embryogenesis
    Zhenglun Zhu; Fiscal Year: 2006
    ..abstract_text> ..
  18. NF-kappaB in myeloma cell growth and survival in vivo
    Babatunde Oyajobi; Fiscal Year: 2007
    ..the finding that expression in myeloma cells, of a dominant-negative (deltaF) mutant of the E3 Ubiquitin ligase betaTrCP/FWD1 that regulates NF-kB signaling, significantly reduced tumor growth in mice in vivo...
  19. Role of PER phosphorylation in circadian biology
    JOANNA CHIU; Fiscal Year: 2006
    ..I will integrate in vitro assays, tissue culture based systems, and whole animal approaches. The use of Drosophila melanogaster as the model animal provides a powerful system for genetic manipulations. ..
  20. Stability of prolactin receptor and prolactin signaling
    Serge Y Fuchs; Fiscal Year: 2010
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..
  21. Stability of prolactin receptor and prolactin signaling
    SERGE FUCHS; Fiscal Year: 2007
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..
  22. Stability of prolactin receptor and prolactin signaling
    SERGE FUCHS; Fiscal Year: 2009
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..