Brn 3b


Gene Symbol: Brn 3b
Description: POU class 4 homeobox 2
Alias: BRN3.2, BRN3B, Brn-3b, POU domain, class 4, transcription factor 2, Brn3b POU domain transcription factor, POU domain protein, brain-3B, brain-specific homeobox/POU domain protein 3B
Species: human
Products:     Brn 3b

Top Publications

  1. Smith M, Dawson S, Latchman D. Inhibition of neuronal process outgrowth and neuronal specific gene activation by the Brn-3b transcription factor. J Biol Chem. 1997;272:1382-8 pubmed
    ..Hence a single amino acid difference results in these closely related factors having opposite effects and allows the balance between them to regulate differentiation. ..
  2. Xiang M, Zhou L, Peng Y, Eddy R, Shows T, Nathans J. Brn-3b: a POU domain gene expressed in a subset of retinal ganglion cells. Neuron. 1993;11:689-701 pubmed
    ..POU domain transcription factors in human retina cDNA has led to the identification of Brn-3b, a class IV POU domain protein. Immunohistochemical experiments show that chicken, mouse, rabbit, monkey, and human retinas contain Brn-3b ..
  3. Xiang M, Zhou L, Macke J, Yoshioka T, Hendry S, Eddy R, et al. The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons. J Neurosci. 1995;15:4762-85 pubmed
  4. Dennis J, Budhram Mahadeo V, Latchman D. The Brn-3b POU family transcription factor regulates the cellular growth, proliferation, and anchorage dependence of MCF7 human breast cancer cells. Oncogene. 2001;20:4961-71 pubmed
    ..These results thus further indicate a role for the Brn-3b transcription factor in regulating mammary cell growth and suggest that its elevation in breast cancer is of functional significance. ..
  5. Samady L, Faulkes D, Budhram Mahadeo V, Ndisang D, Potter E, Brabant G, et al. The Brn-3b POU family transcription factor represses plakoglobin gene expression in human breast cancer cells. Int J Cancer. 2006;118:869-78 pubmed
  6. Irshad S, Pedley R, Anderson J, Latchman D, Budhram Mahadeo V. The Brn-3b transcription factor regulates the growth, behavior, and invasiveness of human neuroblastoma cells in vitro and in vivo. J Biol Chem. 2004;279:21617-27 pubmed
    ..It is possible that decreasing Brn-3b levels may reverse some effects on growth and proliferation of these cells. ..
  7. Budhram Mahadeo V, Irshad S, Bowen S, Lee S, Samady L, Tonini G, et al. Proliferation-associated Brn-3b transcription factor can activate cyclin D1 expression in neuroblastoma and breast cancer cells. Oncogene. 2008;27:145-54 pubmed
    ..Site-directed mutagenesis of this sequence resulted in loss of transactivation of the CD1 promoter by Brn-3b. Thus, Brn-3b may act to alter growth properties of breast cancer and NB cells by enhancing CD1 expression in these cells. ..
  8. Budhram Mahadeo V, Parker M, Latchman D. POU transcription factors Brn-3a and Brn-3b interact with the estrogen receptor and differentially regulate transcriptional activity via an estrogen response element. Mol Cell Biol. 1998;18:1029-41 pubmed
    ..These observations and their implications for transcriptional regulation by the ER are discussed. ..
  9. Samady L, Dennis J, Budhram Mahadeo V, Latchman D. Activation of CDK4 gene expression in human breast cancer cells by the Brn-3b POU family transcription factor. Cancer Biol Ther. 2004;3:317-23 pubmed
    ..The effect of Brn-3b on the growth regulatory CDK4 protein provides a further mechanism by which Brn-3b can regulate breast cancer cell growth and indicates that it can do this by activating as well as repressing specific target genes. ..

More Information


  1. Lee S, Ndisang D, Patel C, Dennis J, Faulkes D, D Arrigo C, et al. Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter. Cancer Res. 2005;65:3072-80 pubmed
    ..Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells. ..
  2. Tsoka P, Barbisan P, Kataoka K, Chen X, Tian B, Bouzika P, et al. NLRP3 inflammasome in NMDA-induced retinal excitotoxicity. Exp Eye Res. 2019;181:136-144 pubmed publisher
    ..of the two aforementioned genes did not alter the survival of the Brn3a+ (brain-specific homeobox/POU domain protein 3A) RGCs in a significant way at 3- or 7-days post injection (long-term phase)...
  3. Umali J, Hawkey Noble A, French C. Loss of foxc1 in zebrafish reduces optic nerve size and cell number in the retinal ganglion cell layer. Vision Res. 2019;156:66-72 pubmed publisher
    ..Our zebrafish model demonstrates that aberrant regulation of RGC number could act in concert with other known glaucoma risk factors to influence the development of congenital and early onset glaucoma due to FOXC1 mutation. ..
  4. Diacou R, Zhao Y, Zheng D, Cvekl A, Liu W. Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation. Cell Rep. 2018;25:2510-2523.e4 pubmed publisher
    ..Therefore, Six3 and Six6 together directly or indirectly suppress Wnt/β-catenin signaling but promote retinogenic factors for the maintenance of multipotent neuroretinal progenitors. ..
  5. Lee V, Hosking B, Holeniewska J, Kubala E, Lundh von Leithner P, Gardner P, et al. BTBR ob/ob mouse model of type 2 diabetes exhibits early loss of retinal function and retinal inflammation followed by late vascular changes. Diabetologia. 2018;61:2422-2432 pubmed publisher
    ..Animals were weighed and blood glucose was measured. TUNEL and brain-specific homeobox/POU domain protein 3A (BRN3A) markers were used to examine retinal ganglion cells...
  6. Budhram Mahadeo V, Fujita R, Bitsi S, Sicard P, Heads R. Co-expression of POU4F2/Brn-3b with p53 may be important for controlling expression of pro-apoptotic genes in cardiomyocytes following ischaemic/hypoxic insults. Cell Death Dis. 2014;5:e1503 pubmed publisher
  7. Li R, Wu F, Ruonala R, Sapkota D, Hu Z, Mu X. Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells. PLoS ONE. 2014;9:e92105 pubmed publisher
  8. Badea T, Williams J, Smallwood P, Shi M, Motajo O, Nathans J. Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis. J Neurosci. 2012;32:995-1007 pubmed publisher
    The three members of the Brn3 family of POU-domain transcription factors (Brn3a/Pou4f1, Brn3b/Pou4f2, and Brn3c/Pou4f3) are expressed in overlapping subsets of visual, auditory/vestibular, and somatosensory neurons...
  9. Wu Y, Jiang G, Zhang N, Liu S, Lin X, Perschon C, et al. HOXA9, PCDH17, POU4F2, and ONECUT2 as a Urinary Biomarker Combination for the Detection of Bladder Cancer in Chinese Patients with Hematuria. Eur Urol Focus. 2018;: pubmed publisher
    ..We found that this urinary biomarker combination may help discriminate bladder cancer from other benign diseases in patients with hematuria, resulting in a reduction of unnecessary invasive examination in patients at low risk. ..
  10. Sun X, Chen M, Li J, Zhuang J, Gao Q, Zhong X, et al. E13.5 retinal progenitors induce mouse bone marrow mesenchymal stromal cells to differentiate into retinal progenitor-like cells. Cytotherapy. 2011;13:294-303 pubmed publisher
    ..expressed immature neural markers but did not express Nfl, Gfap or the retina-related genes Pax6, Math5 and Brn3b. When co-cultured with E13...
  11. Ishii J, Sato H, Yazawa T, Shishido Hara Y, Hiramatsu C, Nakatani Y, et al. Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation. Pathol Int. 2014;64:415-22 pubmed publisher
    ..These findings suggest that the expression of class III/IV POU genes is important for the proneural/neuroendocrine differentiation of lung cancer cells. ..
  12. Kobayashi W, Onishi A, Tu H, Takihara Y, Matsumura M, Tsujimoto K, et al. Culture Systems of Dissociated Mouse and Human Pluripotent Stem Cell-Derived Retinal Ganglion Cells Purified by Two-Step Immunopanning. Invest Ophthalmol Vis Sci. 2018;59:776-787 pubmed publisher
    ..retinas and the ESC-derived 3D retinas could be maintained for approximately 2 to 3 weeks, expressing the markers BRN3B and SMI-312...
  13. Chintala S, Putris N, Geno M. Activation of TLR3 promotes the degeneration of retinal ganglion cells by upregulating the protein levels of JNK3. Invest Ophthalmol Vis Sci. 2015;56:505-14 pubmed publisher
    ..after treatments, degeneration of RGCs was assessed by using antibodies against brain-specific homeobox/POU domain protein 3a (Brn3a). A TLR3-specific inhibitor was injected into the vitreous humor with or without Poly(I:C)...
  14. Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N. Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells. Sci Rep. 2015;5:8344 pubmed publisher
    ..Axons extended radially from the margin of the clump. Induced RGCs expressed specific markers, such as Brn3b and Math5, as assessed using by quantitative PCR and immunohistochemistry...
  15. Roozafzoon R, Lashay A, Vasei M, Ai J, Khoshzaban A, Keshel S, et al. Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network. Biochem Biophys Res Commun. 2015;457:154-60 pubmed publisher
    ..Immuncytochemical and gene expression analysis revealed the expression of Pax6, Atoh7 and BRN3B increased in 3D fibrin culture compared to 2D conventional culture...
  16. Yukita M, Machida S, Nishiguchi K, Tsuda S, Yokoyama Y, Yasuda M, et al. Molecular, anatomical and functional changes in the retinal ganglion cells after optic nerve crush in mice. Doc Ophthalmol. 2015;130:149-56 pubmed publisher
    ..The expression of the RGC markers Brn3a and Brn3b was measured on days 1, 2, 3, 5 and 7 after ONC with quantitative real-time PCR...
  17. Phatak N, Stankowska D, Krishnamoorthy R. Transcription Factor Brn-3b Overexpression Enhances Neurite Outgrowth in PC12 Cells Under Condition of Hypoxia. Cell Mol Neurobiol. 2015;35:769-83 pubmed publisher
    ..The findings have implications for a potential role of Brn-3b in neurodegenerative diseases in which hypoxia/ischemia contribute to pathophysiology of the disease. ..
  18. Badea T, Nathans J. Morphologies of mouse retinal ganglion cells expressing transcription factors Brn3a, Brn3b, and Brn3c: analysis of wild type and mutant cells using genetically-directed sparse labeling. Vision Res. 2011;51:269-79 pubmed publisher
    ..Previous work has shown that the transcription factor Brn3b/Pou4f2 plays a major role in the development and survival of many RGCs...
  19. Stankowska D, Minton A, Rutledge M, Mueller B, Phatak N, He S, et al. Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma. Invest Ophthalmol Vis Sci. 2015;56:893-907 pubmed publisher
    ..b>Brn3b is a class-4 POU domain transcription factor that plays a key role in RGC development, axon outgrowth, and ..
  20. Li F, Su Y, Cheng Y, Jiang X, Peng Y, Li Y, et al. Conditional deletion of Men1 in the pancreatic β-cell leads to glucagon-expressing tumor development. Endocrinology. 2015;156:48-57 pubmed publisher
    ..menin deficient and expressed the mature α-cell-specific transcription factors Brain-specific homeobox POU domain protein 4 (Brn4) and v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (MafB)...
  21. Fernandes K, Bloomsburg S, Miller C, Billingslea S, Merrill M, Burgess R, et al. Novel axon projection after stress and degeneration in the Dscam mutant retina. Mol Cell Neurosci. 2016;71:1-12 pubmed publisher
    ..Further analysis of retinas in which Dscam was conditionally targeted with Brn3b or Pax6α Cre indicated that axon stress and remodeling could occur in the absence of hydrocephalus, which ..
  22. Simmons A, Bloomsburg S, Billingslea S, Merrill M, Li S, Thomas M, et al. Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers. Mol Vis. 2016;22:705-17 pubmed
    ..Unlike other Cre recombinase lines that target retinal neurons, no recombination was observed in Müller or other retinal glia. These properties make this Cre recombinase line a useful tool for vision researchers. ..
  23. Rovere G, Nadal Nicolás F, Sobrado Calvo P, García Bernal D, Villegas Pérez M, Vidal Sanz M, et al. Topical Treatment With Bromfenac Reduces Retinal Gliosis and Inflammation After Optic Nerve Crush. Invest Ophthalmol Vis Sci. 2016;57:6098-6106 pubmed publisher
    ..Quantification of Brn3a (brain-specific homeobox/POU domain protein 3A) +RGCs (retinal ganglion cells) in cross sections showed that bromfenac treatment does not accelerate ONC-..
  24. Mao C, Agca C, Mocko Strand J, Wang J, Ullrich Lüter E, Pan P, et al. Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development. Proc Biol Sci. 2016;283:20152978 pubmed publisher
  25. Ng T, Yung J, Choy K, Cao D, Leung C, Cheung H, et al. Transdifferentiation of periodontal ligament-derived stem cells into retinal ganglion-like cells and its microRNA signature. Sci Rep. 2015;5:16429 pubmed publisher
    ..This study identified the transdifferentiation process of human adult stem cells into retinal ganglion-like cells and revealed the involvement of both genetic and miRNA regulatory mechanisms. ..
  26. Phatak N, Stankowska D, Krishnamoorthy R. Bcl-2, Bcl-xL, and p-AKT are involved in neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma. Mol Vis. 2016;22:1048-61 pubmed
    b>Brn3b is a class IV POU domain transcription factor that plays an important role in the development of retinal ganglion cells (RGCs), RGC survival, and particularly axon growth and pathfinding...
  27. Sajgo S, Ghinia M, Brooks M, Kretschmer F, Chuang K, Hiriyanna S, et al. Molecular codes for cell type specification in Brn3 retinal ganglion cells. Proc Natl Acad Sci U S A. 2017;114:E3974-E3983 pubmed publisher
    ..We use deep sequencing to define (i) transcriptomes of Brn3a- and/or Brn3b-positive RGCs, (ii) Brn3a- and/or Brn3b-dependent RGC transcripts, and (iii) transcriptomes of ..
  28. Ghinia M, Novelli E, Sajgo S, Badea T, Strettoi E. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. J Comp Neurol. 2019;527:187-211 pubmed publisher
    ..Using Brn3a and Brn3b mutant mice in which the alkaline phosphatase gene was knocked-in (Badea et al...
  29. Sajgo S, Ali S, Popescu O, Badea T. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. J Comp Neurol. 2016;524:1033-61 pubmed publisher
    ..Brn3a, Brn3b, and Brn3c, in combination with each other and/or transcription factors of other families, can define subgroups of ..
  30. Bitsi S, Ali H, Maskell L, Ounzain S, Mohamed Ali V, Budhram Mahadeo V. Profound hyperglycemia in knockout mutant mice identifies novel function for POU4F2/Brn-3b in regulating metabolic processes. Am J Physiol Endocrinol Metab. 2016;310:E303-12 pubmed publisher
  31. Qu C, Bian D, Li X, Xiao J, Wu C, Li Y, et al. Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem. 2016;291:7661-8 pubmed publisher
    ..Knockout ofFezf2in the developing retina reduced BRN3B+ cells and increased apoptotic cell markers...
  32. Jain V, Srivastava I, Palchaudhuri S, Goel M, Sinha Mahapatra S, Dhingra N. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS ONE. 2016;11:e0157226 pubmed publisher
    ..Specifically, we looked at a distinct subpopulation of mRGCs that express Brn3b transcription factor, which has been shown to mediate PLR...
  33. Chintalapudi S, Djenderedjian L, Stiemke A, Steinle J, Jablonski M, Morales Tirado V. Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas. Front Aging Neurosci. 2016;8:93 pubmed publisher
    ..labeling of homogeneous sorted cells for the intracellular RGC-markers SNCG, brain-specific homeobox/POU domain protein 3A (BRN3A), TUJ1, and RNA-binding protein with multiple splicing (RBPMS); and (3) by applying the ..
  34. Singh R, Mallela R, Cornuet P, Reifler A, Chervenak A, West M, et al. Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures. Stem Cells Dev. 2015;24:2778-95 pubmed publisher
    ..CRX and RCVRN), inner nuclear layer neurons (expressing CALB2), and retinal ganglion cells [RGCs, expressing BRN3B and Neurofilament (NF) 200]...
  35. Maskell L, Qamar K, Babakr A, Hawkins T, Heads R, Budhram Mahadeo V. Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart. Cell Death Dis. 2017;8:e2861 pubmed publisher
    ..Double morphant embryos targeted with morpholino oligonucleotides to both TFs develop significant cardiac defects (looping abnormalities and valve defects) suggesting essential roles for Brn-3a and Brn-3b in developing hearts. ..
  36. Wang J, Galvão J, Beach K, Luo W, Urrutia R, Goldberg J, et al. Novel Roles and Mechanism for Krüppel-like Factor 16 (KLF16) Regulation of Neurite Outgrowth and Ephrin Receptor A5 (EphA5) Expression in Retinal Ganglion Cells. J Biol Chem. 2016;291:18084-95 pubmed publisher
  37. Chintala S, Daram N. Down-Regulation of RNA 3'-Terminal Phosphate Cyclase Attenuates Toll-Like Receptor 3-Mediated Axonal Loss in the Retina and Optic Nerve. Invest Ophthalmol Vis Sci. 2016;57:5338-5347 pubmed publisher
    ..At 24, 48, and 72 hours after treatments, RGC loss was determined with the brain-specific homeobox/POU domain protein 3a antibody, and axonal loss was assessed by using the neuronal class III beta-tubulin (Tuj1) antibody...
  38. Wu F, Kaczynski T, Sethuramanujam S, Li R, Jain V, Slaughter M, et al. Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate. Proc Natl Acad Sci U S A. 2015;112:E1559-68 pubmed publisher
    ..We further conclude that during development a core group of limited transcription factors, including Pou4f2 and Isl1, function downstream of Atoh7 to determine the RGC fate and initiate RGC differentiation. ..
  39. Qian C, Kaplow M, Lee J, Grueber W. Diversity of Internal Sensory Neuron Axon Projection Patterns Is Controlled by the POU-Domain Protein Pdm3 in Drosophila Larvae. J Neurosci. 2018;38:2081-2093 pubmed publisher
    ..Our results provide an entry point into studying internal sensory circuit development and function, and establish Pdm3 as a regulator of interoceptive axon targeting. ..
  40. Deng F, Chen M, Liu Y, Hu H, Xiong Y, Xu C, et al. Stage-specific differentiation of iPSCs toward retinal ganglion cell lineage. Mol Vis. 2016;22:536-47 pubmed
    ..A subset of transfected cells displayed RGC-specific expression patterns, including Brn3b, iSlet1, calretinin, and Tuj, and approximately 23% of Brn3b-positive RGC-like cells were obtained finally...
  41. Sadakane Y, Hatanaka Y. Photoaffinity electrophoretic mobility shift assay using photoreactive DNA bearing 3-trifluoromethyl-3-phenyldiazirine in its phosphate backbone. Anal Biochem. 2016;506:1-7 pubmed publisher
    ..distinguish the individual binding abilities of three proteins on a single lane and showed that the whole POU domain protein specifically bound to octamer DNA motif by competition experiments...
  42. Zheng D, Yang X, Sheng D, Yu D, Liang G, Guo L, et al. Pou4f2-GFP knock-in mouse line: A model for studying retinal ganglion cell development. Genesis. 2016;54:534-541 pubmed publisher
    ..Thus, the present Pou4f2-GFP knock-in mouse line is a useful tool for further studies on the differentiation and regeneration of RGCs. ..
  43. Parmhans N, Sajgo S, Niu J, Luo W, Badea T. Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret. J Comp Neurol. 2018;526:742-766 pubmed publisher
    ..Ret expression overlaps with Brn3a in 4 RGC types, with Brn3b in 5 RGC types, and with Brn3c in one RGC type, respectively...
  44. de Melo J, Qiu X, Du G, Cristante L, Eisenstat D. Dlx1, Dlx2, Pax6, Brn3b, and Chx10 homeobox gene expression defines the retinal ganglion and inner nuclear layers of the developing and adult mouse retina. J Comp Neurol. 2003;461:187-204 pubmed
    ..5 (Eisenstat et al. [1999] J. Comp. Neurol. 217-237). At E13.5, the expression of four homeodomain proteins, DLX2, BRN3b, PAX6, and CHX10, define distinct yet overlapping domains in the retinal neuroepithelium...
  45. Zhang Q, Zagozewski J, Cheng S, Dixit R, Zhang S, de Melo J, et al. Regulation of Brn3b by DLX1 and DLX2 is required for retinal ganglion cell differentiation in the vertebrate retina. Development. 2017;144:1698-1711 pubmed publisher
    ..We hypothesized that Dlx1, Dlx2 and Brn3b homeobox genes function in parallel intrinsic pathways to determine RGC fate and therefore generated Dlx1
  46. Bhargava A, Li Z, Weissman S. Differential expression of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells. Proc Natl Acad Sci U S A. 1993;90:10260-4 pubmed
    ..Transient transfection of the gene encoding Oct-T1 decreased the activity of the interleukin 2 gene promoter in activated Jurkat cells. Further, there is evidence for an additional octamer-binding protein, Oct-T3, in Jurkat T cells. ..
  47. Fujita R, Ounzain S, Wang A, Heads R, Budhram Mahadeo V. Hsp-27 induction requires POU4F2/Brn-3b TF in doxorubicin-treated breast cancer cells, whereas phosphorylation alters its cellular localisation following drug treatment. Cell Stress Chaperones. 2011;16:427-39 pubmed publisher
  48. Calissano M, Diss J, Latchman D. Post-transcriptional regulation of the Brn-3b transcription factor in differentiating neuroblastoma cells. FEBS Lett. 2007;581:2490-6 pubmed
    ..Moreover we show that Dicer, one of the key enzymes in the production of microRNAs, is strongly up-regulated in ND7 cells subjected to differentiation. ..
  49. Nurminen V, Neme A, Ryynänen J, Heikkinen S, Seuter S, Carlberg C. The transcriptional regulator BCL6 participates in the secondary gene regulatory response to vitamin D. Biochim Biophys Acta. 2015;1849:300-8 pubmed publisher
    ..In conclusion, our study indicated that in monocytes most of the physiological responses to 1,25(OH)2D3 involve the action of the transcription factor BCL6. ..
  50. Zhang L, Wahlin K, Li Y, Masuda T, Yang Z, Zack D, et al. RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors. Mol Vis. 2013;19:1371-86 pubmed
  51. Salichs E, Ledda A, Mularoni L, Albà M, De La Luna S. Genome-wide analysis of histidine repeats reveals their role in the localization of human proteins to the nuclear speckles compartment. PLoS Genet. 2009;5:e1000397 pubmed publisher
    ..These data contribute to our better understanding of the physiological role of single amino acid repeats in proteins. ..
  52. Ring C, Latchman D. The human Brn-3b POU transcription factor shows only limited homology to the Brn-3a/RDC-1 factor outside the conserved POU domain. Nucleic Acids Res. 1993;21:2946 pubmed
  53. Smith A, Rohrer B, Wheless L, Samantaray S, Ray S, Inoue J, et al. Calpain inhibition reduces structural and functional impairment of retinal ganglion cells in experimental optic neuritis. J Neurochem. 2016;139:270-284 pubmed publisher
    ..SNJ 1945 preserves visual acuity and preserves retinal ganglion cells (Brn3a, brain-specific homeobox/POU domain protein 3A) and their axons (MOSP, myelin oligodendrocyte-specific protein)...
  54. Wang Y, Yu Y, Ye R, Zhang D, Li Q, An D, et al. An epigenetic biomarker combination of PCDH17 and POU4F2 detects bladder cancer accurately by methylation analyses of urine sediment DNA in Han Chinese. Oncotarget. 2016;7:2754-64 pubmed publisher
    ..The combination of POU4F2/PCDH17 has yielded the highest sensitivity and specificity of 90.00% and 93.96% in all the 312 individuals, showing the capability of detecting BlCa effectively among pathologically varied sample groups. ..
  55. Huang L, Chen M, Zhang W, Sun X, Liu B, Ge J. Retinoid acid and taurine promote NeuroD1-induced differentiation of induced pluripotent stem cells into retinal ganglion cells. Mol Cell Biochem. 2018;438:67-76 pubmed publisher
    ..that NeuroD1 overexpression increased the expression of retina ganglion cell markers including Islet-1, Math5, Brn3b, and Thy1.2...
  56. Sluch V, Chamling X, Liu M, Berlinicke C, Cheng J, Mitchell K, et al. Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells. Stem Cells Transl Med. 2017;6:1972-1986 pubmed publisher
    ..2, are expressed under the control of the retinal ganglion cell (RGC)-enriched gene BRN3B. Using these reporter cell lines, we greatly improved adherent stem cell differentiation to the RGC lineage by ..
  57. Reinert T, Borre M, Christiansen A, Hermann G, Ørntoft T, Dyrskjøt L. Diagnosis of bladder cancer recurrence based on urinary levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 hypermethylation. PLoS ONE. 2012;7:e46297 pubmed publisher
    ..Methylation levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 in urine specimens are promising diagnostic biomarkers for bladder cancer recurrence surveillance. ..
  58. Qiu F, Jiang H, Xiang M. A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors. J Neurosci. 2008;28:3392-403 pubmed publisher
    ..Here we show that the previously unsuspected POU domain transcription factor Brn3b (brain-specific homeobox/POU domain protein 3b) plays such a critical role...
  59. Erskine L, François U, Denti L, Joyce A, Tillo M, Bruce F, et al. VEGF-A and neuropilin 1 (NRP1) shape axon projections in the developing CNS via dual roles in neurons and blood vessels. Development. 2017;144:2504-2516 pubmed publisher
    ..Targeted NRP1 ablation in RGCs with a Brn3bCre knock-in allele reduced axonal midline crossing at the optic chiasm and optic tract ..
  60. Sayyad Z, Sirohi K, Radha V, Swarup G. 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively. Sci Rep. 2017;7:16855 pubmed publisher
    ..Here, we report that this cell line expressed certain markers specific to retinal ganglion cells such as Rbpms, Brn3b (Pou4f2), Brn3c (Pou4f3), Thy1 and ?-synuclein (Sncg), and some other markers of neuronal cells (beta-III tubulin, ..
  61. Ounzain S, Bowen S, Patel C, Fujita R, Heads R, Budhram Mahadeo V. Proliferation-associated POU4F2/Brn-3b transcription factor expression is regulated by oestrogen through ER? and growth factors via MAPK pathway. Breast Cancer Res. 2011;13:R5 pubmed publisher
  62. Weishaupt J, Klocker N, Bahr M. Axotomy-induced early down-regulation of POU-IV class transcription factors Brn-3a and Brn-3b in retinal ganglion cells. J Mol Neurosci. 2005;26:17-25 pubmed