BLAP75

Summary

Gene Symbol: BLAP75
Description: RecQ mediated genome instability 1
Alias: BLAP75, C9orf76, FAAP75, BLM-associated polypeptide, 75 kDa, BLM-associated protein 75 kDa, RMI1, RecQ mediated genome instability 1, homolog, homolog of yeast RecQ-mediated genome instability 1 (RMI1), recQ-mediated genome instability protein 1
Species: human

Top Publications

  1. pmc BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity
    Jinhu Yin
    Laboratory of Genetics, National Institute on Aging, National Institute of Health, Baltimore, MD 21224, USA
    EMBO J 24:1465-76. 2005
  2. pmc BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome
    Thiyam Ramsing Singh
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Genes Dev 22:2856-68. 2008
  3. ncbi Genetic variant of the human homologous recombination-associated gene RMI1 (S455N) impacts the risk of AML/MDS and malignant melanoma
    Karin Broberg
    Department of Occupational and Environmental Medicine, Lund University Hospital, SE 221 85, Lund, Sweden
    Cancer Lett 258:38-44. 2007
  4. doi Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1
    Nicolas Bocquet
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Nat Struct Mol Biol 21:261-8. 2014
  5. pmc The dissolution of double Holliday junctions
    Anna H Bizard
    Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, 2200 Copenhagen N, Denmark
    Cold Spring Harb Perspect Biol 6:a016477. 2014
  6. pmc Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe
    Shriparna Sarbajna
    Clare Hall Laboratories, Cancer Research UK, London Research Institute, Herts EN6 3LD, United Kingdom
    Genes Dev 28:1124-36. 2014
  7. ncbi Genome-wide association study (GWAS) for molar-incisor hypomineralization (MIH)
    Jan Kuhnisch
    Department of Conservative Dentistry and Periodontology, School of Dentistry, Ludwig Maximilians University of Munich, Goethestraße70, Munich, 80336, Germany
    Clin Oral Investig 18:677-82. 2014
  8. ncbi DNA2 Cooperates with the WRN and BLM RecQ Helicases to Mediate Long-range DNA End Resection in Human Cells
    Andreas Sturzenegger
    From the Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland and
    J Biol Chem 289:27314-26. 2014
  9. doi Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex
    R Sugahara
    Laboratory of Silkworm Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan
    Insect Mol Biol 23:185-98. 2014
  10. doi Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1
    Mong Sing Lai
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem Biophys Res Commun 427:682-6. 2012

Scientific Experts

  • DAVID SWEETSER
  • Akira Suwa
  • Karin Broberg
  • Holger Puchta
  • Haico van Attikum
  • Kirill S Lobachev
  • M Grelon
  • GEORGE CROSS
  • Richard Kolodner
  • PATRICK M SUNG
  • J Kuhnisch
  • Ali Yavuzcan
  • Kristina H Schmidt
  • Bernd Frank
  • Kara A Bernstein
  • Steven J Brill
  • Ian D Hickson
  • RODNEY J ROTHSTEIN
  • Ilya J Finkelstein
  • Valeria Busygina
  • Petr Cejka
  • Weidong Wang
  • Amom Ruhikanta Meetei
  • Hocine W Mankouri
  • Jay Yang
  • Csanad Z Bachrati
  • Steven Raynard
  • Thiyam Ramsing Singh
  • Grant W Brown
  • Clare L Fasching
  • Steven Brill
  • KARA BERNSTEIN
  • CLARE FASCHING
  • Stephen C West
  • Stephen C Kowalczykowski
  • Kelly A Hoadley
  • Mong Sing Lai
  • Chi Fu Chen
  • Anna H Bizard
  • Chen Ling
  • Zhijiang Yan
  • Wendy Bussen
  • Yu Zhang
  • Jody L Plank
  • Thomas M Ashton
  • Abdullah Mahmood Ali
  • Grzegorz Ira
  • Eun Yong Shim
  • Woo Hyun Chung
  • Zhu Zhu
  • Hengyao Niu
  • Yutong Xue
  • Frank Hartung
  • Takemi Enomoto
  • Alexandra Sobeck
  • Dongyi Xu
  • Maureen E Hoatlin
  • Lei Li
  • Hans Joenje
  • Johan P de Winter
  • Shriparna Sarbajna
  • Andreas Sturzenegger
  • R Sugahara
  • Nicolas Bocquet
  • Indrajit Chaudhury
  • Arun Pradhan
  • Michel F Guiraldelli
  • M Rebecca Glineburg
  • Michael Chang
  • Simone Bonnet
  • Xiaoyu Xue
  • Jinhu Yin
  • Jessica A Kennedy
  • Jiang Chen
  • Thomas Costelloe
  • Stefanie Hartman Chen
  • Kebola Wahengbam
  • Eleni P Mimitou
  • H Chen
  • Hee Sook Kim
  • Xuefeng Chen
  • James L Keck
  • Thomas Wechsler
  • Alexander Knoll
  • Sang Eun Lee
  • Akhilesh K Singh
  • Feng Wang
  • Maren Mönnich
  • Masayuki Seki
  • Andrew J Deans

Detail Information

Publications76

  1. pmc BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity
    Jinhu Yin
    Laboratory of Genetics, National Institute on Aging, National Institute of Health, Baltimore, MD 21224, USA
    EMBO J 24:1465-76. 2005
    ..Here we demonstrate that BLAP75, a novel protein containing a putative OB-fold nucleic acid binding domain, is an integral component of BLM ..
  2. pmc BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome
    Thiyam Ramsing Singh
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Genes Dev 22:2856-68. 2008
    ..BLM encodes a DNA helicase of the RECQ family, and associates with Topo IIIalpha and BLAP75/RMI1 (BLAP for BLM-associated polypeptide/RecQ-mediated genome instability) to form the BTB (BLM-Topo IIIalpha-..
  3. ncbi Genetic variant of the human homologous recombination-associated gene RMI1 (S455N) impacts the risk of AML/MDS and malignant melanoma
    Karin Broberg
    Department of Occupational and Environmental Medicine, Lund University Hospital, SE 221 85, Lund, Sweden
    Cancer Lett 258:38-44. 2007
    The newly identified protein BLAP75/RMI1 associates with the helicase BLM and is critical for the function of the homologous recombination complex...
  4. doi Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1
    Nicolas Bocquet
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Nat Struct Mol Biol 21:261-8. 2014
    ..Our results provide a mechanistic rationale for how RMI1 stabilizes TopIIIα-gate opening to enable dissolution and illustrate how binding partners modulate topoisomerase function. ..
  5. pmc The dissolution of double Holliday junctions
    Anna H Bizard
    Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, 2200 Copenhagen N, Denmark
    Cold Spring Harb Perspect Biol 6:a016477. 2014
    ..This review details our current knowledge of the dissolution process and the players involved in catalyzing this mechanistically complex means of completing homologous recombination reactions. ..
  6. pmc Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe
    Shriparna Sarbajna
    Clare Hall Laboratories, Cancer Research UK, London Research Institute, Herts EN6 3LD, United Kingdom
    Genes Dev 28:1124-36. 2014
    ..These observations demonstrate that the SLX1-SLX4 nuclease and the SLX4 scaffold play divergent roles in the maintenance of genome integrity in human cells. ..
  7. ncbi Genome-wide association study (GWAS) for molar-incisor hypomineralization (MIH)
    Jan Kuhnisch
    Department of Conservative Dentistry and Periodontology, School of Dentistry, Ludwig Maximilians University of Munich, Goethestraße70, Munich, 80336, Germany
    Clin Oral Investig 18:677-82. 2014
    ..Clinical and genetic data from the 10-year follow-up of 668 children from the Munich GINI-plus and LISA-plus birth cohort studies were analyzed...
  8. ncbi DNA2 Cooperates with the WRN and BLM RecQ Helicases to Mediate Long-range DNA End Resection in Human Cells
    Andreas Sturzenegger
    From the Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland and
    J Biol Chem 289:27314-26. 2014
    ..Our study provides new mechanistic insights into the process of DNA end resection in mammalian cells...
  9. doi Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex
    R Sugahara
    Laboratory of Silkworm Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan
    Insect Mol Biol 23:185-98. 2014
    ..Our results suggest that BmFancM also acts as a platform for recruitment of both the FA protein and the BS protein, although the silkworm genome seems to lose FAAP24, a FancM-binding partner protein in mammals. ..
  10. doi Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1
    Mong Sing Lai
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem Biophys Res Commun 427:682-6. 2012
    ..On the other hand, Rmi1 seemed to function in a pathway involving the Ctf18-RFC complex and Mrc1, which were previously predicted to regulate leading-strand DNA replication...
  11. pmc Role of replication protein A in double holliday junction dissolution mediated by the BLM-Topo IIIα-RMI1-RMI2 protein complex
    Xiaoyu Xue
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 288:14221-7. 2013
    ..Examination of these mutants ascertains the significance of the RMI1-RPA interaction in dHJ dissolution. Our results thus implicate RPA as a cofactor of the BTR complex in dHJ dissolution...
  12. ncbi Gene expression profiles of small-cell lung cancers: molecular signatures of lung cancer
    Masaya Taniwaki
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
    Int J Oncol 29:567-75. 2006
    ..TFCP2L4, PHF20, LMO4, TCF20, RFX2, and DKFZp547I048 as well as those encoding nucleotide-binding proteins such as C9orf76, EHD3, and GIMAP4...
  13. pmc The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection
    Thomas Costelloe
    Department of Toxicogenetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands
    Nature 489:581-4. 2012
    ..These findings unveil an evolutionarily conserved role for the Fun30 and SMARCAD1 chromatin remodellers in controlling end resection, homologous recombination and genome stability in the context of chromatin...
  14. doi A catalytic and non-catalytic role for the Yen1 nuclease in maintaining genome integrity in Kluyveromyces lactis
    Jiang Chen
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, Stockholm, Sweden
    DNA Repair (Amst) 11:833-43. 2012
    ..In addition, yen1 mutants had normal nonhomologous end-joining efficiency. We discuss the similarities and differences between K. lactis Yen1 and Yen1/GEN1 from other organisms...
  15. pmc Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes
    Petr Cejka
    Departments of Microbiology and Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616 8665, USA
    Mol Cell 47:886-96. 2012
    ..This concerted activity of the Sgs1-Top3-Rmi1-RPA represents an important mechanism for disentangling structures resulting from the topological features of duplex DNA...
  16. pmc RMI1 promotes DNA replication fork progression and recovery from replication fork stress
    Jay Yang
    Department of Biochemistry and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
    Mol Cell Biol 32:3054-64. 2012
    ..These findings reveal direct roles of RMI1 in DNA replication and the replication stress response, which could explain the molecular basis for its involvement in suppressing sister chromatid exchange and tumorigenesis...
  17. pmc Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution
    Stefanie Hartman Chen
    Department of Biochemistry, Duke University, Durham, North Carolina 27710, USA
    J Biol Chem 287:19346-53. 2012
    ..Our data therefore suggest that the Top3α C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA) and an essential function in vivo...
  18. pmc Defining the molecular interface that connects the Fanconi anemia protein FANCM to the Bloom syndrome dissolvasome
    Kelly A Hoadley
    Department of Biomolecular Chemistry, 420 Henry Mall, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 109:4437-42. 2012
    ..This study provides a molecular view of the RMI/FANCM complex and highlights a key interface utilized in coordinating the activities of two critical eukaryotic DNA-damage repair machines...
  19. pmc FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery
    Indrajit Chaudhury
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 41:6444-59. 2013
    ..In contrast, FANCI is dispensable for FANCD2-dependent BLMcx regulation, demonstrating functional separation of FANCD2 from FANCI. ..
  20. pmc Genome instability and embryonic developmental defects in RMI1 deficient mice
    Michel F Guiraldelli
    Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    DNA Repair (Amst) 12:835-43. 2013
    ..Our results demonstrate the importance of RMI1 in maintaining genome integrity and normal embryonic development. ..
  21. pmc BLM and RMI1 alleviate RPA inhibition of TopoIIIα decatenase activity
    Jay Yang
    Department of Biochemistry and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 7:e41208. 2012
    ..We propose that topoisomerase IIIα and RPA compete to bind to single-stranded regions of catenanes. Interactions with BLM and RMI1 enhance toposiomerase IIIα activity, promoting decatenation in the presence of RPA...
  22. ncbi Roles of the Bloom's syndrome helicase in the maintenance of genome stability
    C F Cheok
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK
    Biochem Soc Trans 33:1456-9. 2005
    ..a multienzyme complex including topoisomerase IIIalpha, replication protein A and a newly identified factor called BLAP75. Together, these proteins play a role in the resolution of DNA structures that arise during the process of ..
  23. pmc Genome-wide screen reveals replication pathway for quasi-palindrome fragility dependent on homologous recombination
    Yu Zhang
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America
    PLoS Genet 9:e1003979. 2013
    ..These data demonstrate that Rad51 is required for the formation of breakage-prone secondary structures in situations when replication is compromised while another mechanism operates in DSB formation in replication-proficient strains. ..
  24. pmc A transient α-helical molecular recognition element in the disordered N-terminus of the Sgs1 helicase is critical for chromosome stability and binding of Top3/Rmi1
    Jessica A Kennedy
    Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620, USA, Center for Drug Discovery and Innovation, University of South Florida, Tampa, FL 33612, USA and Cancer Biology and Evolution Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Nucleic Acids Res 41:10215-27. 2013
    ....
  25. ncbi Should cut-off values of the risk of malignancy index be changed for evaluation of adnexal masses in Asian and Pacific populations?
    Ali Yavuzcan
    Department of Obstetrics and Gynaecology, Duzce University Faculty of Medicine, Duzce, Turkey E mail
    Asian Pac J Cancer Prev 14:5455-9. 2013
    ..The cut off value of RMI-4 to differentiate benign from malignant lesions is 450. Our aim was to evaluate the efficiency of four different malignancy indexes (RMI1-4) in a homogeneous population...
  26. pmc Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III α-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation duri
    Arun Pradhan
    From the Experimental Hematology and Cancer Biology and Cancer and Blood Diseases Institute, Cincinnati Children s Hospital Medical Center CCHMC, Cincinnati, Ohio 45229
    J Biol Chem 288:33500-8. 2013
    ..Overall, this study suggests that the phosphorylation of the BTR complex is essential to maintain a stable genome. ..
  27. pmc Resolution by unassisted Top3 points to template switch recombination intermediates during DNA replication
    M Rebecca Glineburg
    From the Department of Pathology and Laboratory Medicine
    J Biol Chem 288:33193-204. 2013
    ....
  28. pmc Different functions for the domains of the Arabidopsis thaliana RMI1 protein in DNA cross-link repair, somatic and meiotic recombination
    Simone Bonnet
    Karlsruhe Institute of Technology, Botanical Institute II, Hertzstrasse 16, 76187 Karlsruhe, Germany
    Nucleic Acids Res 41:9349-60. 2013
    ..The OB-fold domain 2 is not necessary for either somatic or meiotic HR, but it seems to have a minor function in DNA cross-link repair. ..
  29. pmc Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3
    Petr Cejka
    Department of Microbiology, University of California, Davis, Davis, California, USA
    Nat Struct Mol Biol 17:1377-82. 2010
    ..Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity...
  30. doi Adipocyte hyperplasia and RMI1 in the treatment of obesity
    Akira Suwa
    Drug Discovery Research, Astellas Pharma Inc, Ibaraki, Japan
    FEBS J 278:565-9. 2011
    ....
  31. pmc DNA end resection--unraveling the tail
    Eleni P Mimitou
    Department of Microbiology and Immunology, Columbia University Medical Center, 701 W 168th St, New York, NY 10032, USA
    DNA Repair (Amst) 10:344-8. 2011
    ..Here we review recent in vitro and in vivo findings that shed more light into the mechanisms of DSB processing in mitotic and meiotic DSB repair as well as in telomere metabolism...
  32. pmc RMI1 attenuates tumor development and is essential for early embryonic survival
    H Chen
    Department of Experimental Radiation Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Carcinog 50:80-8. 2011
    RMI1/BLAP75 (RecQ-mediated genome instability 1/Bloom-associated protein 75) is an OB-fold protein highly conserved from yeast to human...
  33. pmc Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae
    Thomas M Ashton
    Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute 18 1, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Mol Cell Biol 31:1921-33. 2011
    ..We propose that the Sgs1-Top3-Rmi1 complex constitutes the main pathway for the processing of HJ-containing HRR intermediates but that Mus81-Mms4 can also resolve these intermediates...
  34. pmc Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage
    Hocine W Mankouri
    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Proc Natl Acad Sci U S A 108:4944-9. 2011
    ..We propose that HJ resolvases and Sgs1-Top3-Rmi1 comprise two independent processes to deal with HJ-containing DNA intermediates arising during HRR in S-phase...
  35. pmc Aberrant chromosome morphology in human cells defective for Holliday junction resolution
    Thomas Wechsler
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 471:642-6. 2011
    ....
  36. doi Glucose regulates RMI1 expression through the E2F pathways in adipose cells
    Akira Suwa
    Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc, 21 Miyukigaoka, Tsukuba shi, Ibaraki, 305 8585, Japan
    Endocrine 40:56-61. 2011
    ..Further, knockdown of either E2F5 or E2F8 mRNA using siRNA suppressed this glucose-induced up-regulation of RMI1 expression. These results suggest that RMI1 expression may be regulated by glucose, at least in part, via E2F expression...
  37. pmc Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation
    Xuefeng Chen
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Nat Struct Mol Biol 18:1015-9. 2011
    ..Poorly recruited dna2T4A S17A S237A and dna2ΔN248 mutant proteins promote resection only in the presence of Exo1, suggesting cross-talk between Dna2- and Exo1-dependent resection pathways...
  38. ncbi Processing of homologous recombination repair intermediates by the Sgs1-Top3-Rmi1 and Mus81-Mms4 complexes
    Ian D Hickson
    Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark
    Cell Cycle 10:3078-85. 2011
    ..In this review, we discuss the implications of these results and review the putative roles for the Sgs1-Top3-Rmi1 and Mus81-Mms4 complexes in the processing of various types of HRR intermediates during S phase...
  39. pmc Identification of Trypanosoma brucei RMI1/BLAP75 homologue and its roles in antigenic variation
    Hee Sook Kim
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York, United States of America
    PLoS ONE 6:e25313. 2011
    ....
  40. ncbi A novel ubiquitin ligase is deficient in Fanconi anemia
    Amom Ruhikanta Meetei
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
    Nat Genet 35:165-70. 2003
    ..Our data suggest that PHF9 has a crucial role in the Fanconi anemia pathway as the likely catalytic subunit required for monoubiquitination of FANCD2...
  41. pmc Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks
    Eun Yong Shim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
    EMBO J 29:3370-80. 2010
    ..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation...
  42. ncbi Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex
    Feng Wang
    Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Structure 18:1159-70. 2010
    ..We also revealed the structural basis of the interaction between RMI1C-OB and RMI2-OB and demonstrated the functional importance of the RMI1-RMI2 interaction in genome stability maintenance...
  43. pmc Structure and cellular roles of the RMI core complex from the bloom syndrome dissolvasome
    Kelly A Hoadley
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, 550 Medical Science Center, 1300 University Avenue, Madison, WI 53706 1532, USA
    Structure 18:1149-58. 2010
    ..The RMI core interface is therefore crucial for BLM dissolvasome assembly and may have additional cellular roles as a docking hub for other proteins...
  44. pmc RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability
    Dongyi Xu
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, Baltimore, Maryland 21224, USA
    Genes Dev 22:2843-55. 2008
    ..Our data suggest that multi-OB-fold complexes mediate two modes of BLM action: via RPA-mediated protein-DNA interaction, and via RMI-mediated protein-protein interactions...
  45. pmc More complexity to the Bloom's syndrome complex
    Yilun Liu
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Genes Dev 22:2737-42. 2008
    ..RMI2 may be a representative of a new family of OB-fold-containing proteins that are important for complex stabilization and checkpoint response...
  46. pmc Functional role of BLAP75 in BLM-topoisomerase IIIalpha-dependent holliday junction processing
    Steven Raynard
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 283:15701-8. 2008
    The BLAP75 protein combines with the BLM helicase and topoisomerase (Topo) IIIalpha to form an evolutionarily conserved complex, termed the BTB complex, that functions to regulate homologous recombination...
  47. ncbi The RecQ helicase-topoisomerase III-Rmi1 complex: a DNA structure-specific 'dissolvasome'?
    Hocine W Mankouri
    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Trends Biochem Sci 32:538-46. 2007
    ....
  48. ncbi Holliday junction processing activity of the BLM-Topo IIIalpha-BLAP75 complex
    Wendy Bussen
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 282:31484-92. 2007
    ..BLM is stably associated with topoisomerase IIIalpha (Topo IIIalpha) and the BLAP75 protein...
  49. pmc Binding and activation of DNA topoisomerase III by the Rmi1 subunit
    Chi Fu Chen
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA
    J Biol Chem 282:28971-9. 2007
    ..These results demonstrate that Top3-Rmi1 functions as a complex and suggest that Rmi1 stimulates Top3 by promoting its interaction with ssDNA...
  50. pmc Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3
    Hocine W Mankouri
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, United Kingdom
    Mol Biol Cell 18:4062-73. 2007
    ..We propose a model in which the Shu proteins act in HRR to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex...
  51. pmc BLM is required for faithful chromosome segregation and its localization defines a class of ultrafine anaphase bridges
    Kok Lung Chan
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 26:3397-409. 2007
    ..localizes to anaphase bridges, where it colocalizes with its cellular partners, topoisomerase IIIalpha and hRMI1 (BLAP75)...
  52. pmc Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion
    Mong Sing Lai
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai, Miyagi 980 8578, Japan
    EMBO Rep 8:685-90. 2007
    ..Loss of RAD51 also suppresses the cohesion defect of rmi1 mutant cells. These results indicate the existence of a new pathway involving Rad51 and Sgs1-Top3-Rmi1, which leads to the establishment of sister chromatid cohesion...
  53. ncbi Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM
    Alberto Ciccia
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
    Mol Cell 25:331-43. 2007
    ..Our data indicate that the FANCM/FAAP24 complex may play a key role in recruitment of the FA core complex to damaged DNA...
  54. pmc Sgs1 regulates gene conversion tract lengths and crossovers independently of its helicase activity
    Yi Chen Lo
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Mol Cell Biol 26:4086-94. 2006
    ..We propose that Sgs1 has helicase-dependent functions in replication and helicase-independent functions in DSB repair by HR...
  55. ncbi A double Holliday junction dissolvasome comprising BLM, topoisomerase IIIalpha, and BLAP75
    Steven Raynard
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:13861-4. 2006
    ..cellular protein complexes, all of which contain topoisomerase IIIalpha (Topo IIIalpha) and a novel protein BLAP75. Here, using highly purified human proteins, we show that BLAP75 associates independently with both Topo IIIalpha ..
  56. pmc BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediates
    Leonard Wu
    Cancer Research UK, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Proc Natl Acad Sci U S A 103:4068-73. 2006
    ..Here we show that a recently identified third component of the human BLM/hTOPO IIIalpha complex, BLAP75/RMI1, promotes dissolution catalyzed by hTOPO IIIalpha...
  57. ncbi Delineation of the minimal commonly deleted segment and identification of candidate tumor-suppressor genes in del(9q) acute myeloid leukemia
    David A Sweetser
    Department of Pediatrics, Massachusetts General Hospital, 55 Fruit Street Jackson 904, Boston, MA 02114, USA
    Genes Chromosomes Cancer 44:279-91. 2005
    ..genes, FRMD3, UBQLN1, GKAP42, KIF27, HNRPK, SLC28A3, and NTRK2, and 4 novel genes, RASEF, C9orf103, C9orf64, and C9orf76. In addition, TLE1 and TLE4 are adjacent to the CDR...
  58. pmc Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex
    Janet R Mullen
    Department of Molecular Biology and Biochemistry, Rutgers University, CABM, 679 Hoes Ln, Piscataway, NJ 08854, USA
    Mol Cell Biol 25:4476-87. 2005
    ..We propose that the DNA binding specificity of Rmi1 plays a role in targeting Sgs1-Top3 to appropriate substrates...
  59. pmc The Arabidopsis BLAP75/Rmi1 homologue plays crucial roles in meiotic double-strand break repair
    Liudmila Chelysheva
    INRA de Versailles, Institut Jean Pierre Bourgin, Station de Génétique et d Amélioration des Plantes UR 254, Versailles, France
    PLoS Genet 4:e1000309. 2008
    In human cells and in Saccharomyces cerevisiae, BLAP75/Rmi1 acts together with BLM/Sgs1 and TopoIIIalpha/Top3 to maintain genome stability by limiting crossover (CO) formation in favour of NCO events, probably through the dissolution of ..
  60. pmc Topoisomerase 3alpha and RMI1 suppress somatic crossovers and are essential for resolution of meiotic recombination intermediates in Arabidopsis thaliana
    Frank Hartung
    Botany II, University of Karlsruhe, Karlsruhe, Germany
    PLoS Genet 4:e1000285. 2008
    ..that Topoisomerase 3alpha (TOP3alpha) functions together with a RecQ DNA helicase and a third partner, RMI1/BLAP75, in the resolution step of homologous recombination in a process called Holliday Junction dissolution in ..
  61. pmc DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2
    Petr Cejka
    Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
    Nature 467:112-6. 2010
    ..We suggest that Top3-Rmi1 and MRX are important for recruitment of the Sgs1-Dna2 complex to DSBs. Our experiments provide a mechanistic framework for understanding the initial steps of recombinational DNA repair in eukaryotes...
  62. pmc Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae
    Hengyao Niu
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 467:108-11. 2010
    ..Our reconstituted system should serve as a useful model for delineating the mechanistic intricacy of the DNA break resection process in eukaryotes...
  63. ncbi Developing T lymphocytes are uniquely sensitive to a lack of topoisomerase III alpha
    Maren Mönnich
    Department of Developmental Immunology, Max Planck Institute of Immunobiology, Freiburg, Germany
    Eur J Immunol 40:2379-84. 2010
    ..Deficiency in top3a activates a p53-dependent check-point but does not affect VDJ recombination. Our results suggest that TOP3A could be a candidate gene involved in human primary immunodeficiency syndromes...
  64. pmc Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1
    Jay Yang
    Department of Biochemistry and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada and
    J Biol Chem 285:21426-36. 2010
    ....
  65. pmc An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs
    Chi Fu Chen
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ, USA
    EMBO J 29:1713-25. 2010
    ..This work identifies SE as a new molecular function of BLM/Sgs1, and we propose that at least one role of SE is to mediate the strand-passage events catalysed by Top3-Rmi1...
  66. pmc MHF1-MHF2, a histone-fold-containing protein complex, participates in the Fanconi anemia pathway via FANCM
    Thiyam Ramsing Singh
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation and University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Mol Cell 37:879-86. 2010
    ..These findings reveal critical roles of the MHF1-MHF2 dimer in DNA damage repair and genome maintenance through FANCM...
  67. pmc A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability
    Zhijiang Yan
    Laboratory of Genetics, National Institute of Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Mol Cell 37:865-78. 2010
    ..Thus, FANCM-MHF is an essential DNA-remodeling complex that protects replication forks from yeast to human...
  68. doi FANCM connects the genome instability disorders Bloom's Syndrome and Fanconi Anemia
    Andrew J Deans
    London Research Institute, Cancer Research UK, South Mimms, UK
    Mol Cell 36:943-53. 2009
    ..Moreover, a common phenotype of BS and FA cells-an elevated frequency of sister chromatid exchanges-was due to a loss of interaction of the two complexes through FANCM...
  69. doi RMI1 deficiency in mice protects from diet and genetic-induced obesity
    Akira Suwa
    Department of Metabolic Diseases, Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc, Tsukuba, Ibaraki, Japan
    FEBS J 277:677-86. 2010
    ..Taken together, these findings demonstrate that RMI1 is a novel molecule that regulates energy homeostasis...
  70. doi Colorectal cancer and polymorphisms in DNA repair genes WRN, RMI1 and BLM
    Bernd Frank
    Division of Clinical Epidemiology and Aging Research, German Cancer Research Center, 69115 Heidelberg, Germany
    Carcinogenesis 31:442-5. 2010
    ..We investigated associations of WRN, BLM and BLM-associated protein (BLAP75/RMI1) gene polymorphisms and risk of colorectal cancer (CRC), genotyping WRN V114I (rs2230009), WRN L1074F (..
  71. doi Predisposition for TMPRSS2-ERG fusion in prostate cancer by variants in DNA repair genes
    Manuel Luedeke
    1Institute of Human Genetics, University Hospital Ulm, Ulm, Germany
    Cancer Epidemiol Biomarkers Prev 18:3030-5. 2009
    ..02; OR, 1.33; 95% CI, 1.04-1.70) in unselected PCa cases. The DNA repair genes POLI and ESCO1 are proposed as susceptibility genes for TMPRSS2-ERG fusion-positive PCa that warrant further investigation...
  72. doi Dissolution of double Holliday junctions by the concerted action of BLM and topoisomerase IIIalpha
    Csanad Z Bachrati
    Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
    Methods Mol Biol 582:91-102. 2009
    ..In this chapter, we provide a detailed experimental protocol for the preparation of an oligonucleotide-based, double Holliday junction substrate and for the biochemical analysis of dissolution in vitro...
  73. pmc Specific pathways prevent duplication-mediated genome rearrangements
    Christopher D Putnam
    Ludwig Institute for Cancer Research, Department of Medicine, University of California School of Medicine, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0669, USA
    Nature 460:984-9. 2009
    ..This explains how extensive genome instability is prevented in eukaryotic cells whose genomes contain numerous divergent repeated sequences...
  74. pmc Association between polymorphisms in RMI1, TOP3A, and BLM and risk of cancer, a case-control study
    Karin Broberg
    Department of Occupational and Environmental Medicine, Lund University, Lund, Sweden
    BMC Cancer 9:140. 2009
    ..Mutations altering BLM function are associated with highly elevated cancer susceptibility (Bloom syndrome). Thus, genetic variants of BLM and proteins that form complexes with BLM, such as TOP3A and RMI1, might affect cancer risk as well...
  75. pmc Sgs1 function in the repair of DNA replication intermediates is separable from its role in homologous recombinational repair
    Kara A Bernstein
    Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA
    EMBO J 28:915-25. 2009
    ..Taken together, these results suggest that the Sgs1 function in repair of DNA replication intermediates can be uncoupled from its role in homologous recombinational repair...
  76. pmc RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex
    Michael Chang
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO J 24:2024-33. 2005
    ..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response...

Research Grants67

  1. Genomic Stability and RecQ DNA Helicases in Yeast
    Steven J Brill; Fiscal Year: 2010
    ..Thus, this research will provide new understanding about the factors and genetic pathways that maintain genome stability in normal and BS cells. ..
  2. Mechanism of the BLM/Sgs1 Helicase Complex
    Steven J Brill; Fiscal Year: 2013
    ..Successful completion of these experiments will reveal the function of non-helicase domains of the RecQ family and the potential substrates of BTR/STR during DNA repair in vivo. ..
  3. Mechanism of the BLM/Sgs1 Helicase Complex
    Steven J Brill; Fiscal Year: 2013
    ..Successful completion of these experiments will reveal the function of non-helicase domains of the RecQ family and the potential substrates of BTR/STR during DNA repair in vivo. ..
  4. Role of Sgs1 in homologous recombination: Dissolution of branched DNA substrates
    Clare L Fasching; Fiscal Year: 2010
    ..Understanding the role of Sgs1 in homologous recombination may uncover more specific therapeutic target areas on which to focus in the future. ..
  5. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    Kara A Bernstein; Fiscal Year: 2010
    ..Functional analysis of proteins required for DNA repair is crucial for understanding the molecular basis of tumorigenesis. ..
  6. MECHANISM OF SPONTANEOUS AND DSB-INDUCED REPAIR
    RODNEY J ROTHSTEIN; Fiscal Year: 2013
    ..It is these combined genetic and cell biological approaches to the many issues related to the recognition and repair of DNA damage In yeast that will continue to yield insights into this important biological process. ..
  7. Mechanisms of chromatin remodeling and roadblock clearance by DNA motor proteins
    Ilya J Finkelstein; Fiscal Year: 2013
    ..My ultimate career goal is to achieve tenure as a professor at a research institution. The skills that I develop during the K99 phase of the fellowship will enable me to succeed as an independent investigator. ..
  8. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    Kara A Bernstein; Fiscal Year: 2012
    ..abstract_text> ..
  9. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    Kara A Bernstein; Fiscal Year: 2012
    ..abstract_text> ..
  10. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    Kara A Bernstein; Fiscal Year: 2013
    ..abstract_text> ..
  11. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    Kara A Bernstein; Fiscal Year: 2013
    ..abstract_text> ..
  12. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    Kara A Bernstein; Fiscal Year: 2011
    ..abstract_text> ..
  13. Genomic Stability and RecQ DNA Helicases in Yeast
    Steven Brill; Fiscal Year: 2009
    ..Thus, this research will provide new understanding about the factors and genetic pathways that maintain genome stability in normal and BS cells. ..
  14. BLM-mediated Homologous Recombination Regulation and Tumor Suppression
    Patrick Sung; Fiscal Year: 2009
    ..BLM also associates with a novel polypeptide BLAP75 and binds the hRad51 recombinase...
  15. BLM-mediated Homologous Recombination Regulation and Tumor Suppression
    Patrick Sung; Fiscal Year: 2010
    ..BLM also associates with a novel polypeptide BLAP75 and binds the hRad51 recombinase...
  16. BLM-mediated Homologous Recombination Regulation and Tumor Suppression
    Patrick Sung; Fiscal Year: 2007
    ..BLM also associates with a novel polypeptide BLAP75 and binds the hRad51 recombinase...
  17. Role of BLM protein complex in the maintenance of genome integrity
    Valeria Busygina; Fiscal Year: 2007
    ..ours have found that BLM works in conjunction with topoisomerase Ilia (Topo Ilia) and the newly identified protein BLAP75 to mediate the dissolution of the double Holliday junction (DHJ) to form exclusively non-crossover recombinants...
  18. Mechanistic insights into the SHU complex and Sgs1 in DNA repair and replication
    KARA BERNSTEIN; Fiscal Year: 2009
    ..Functional analysis of proteins required for DNA repair is crucial for understanding the molecular basis of tumorigenesis. ..
  19. Role of Sgs1 in homologous recombination: Dissolution of branched DNA substrates
    CLARE FASCHING; Fiscal Year: 2009
    ..Understanding the role of Sgs1 in homologous recombination may uncover more specific therapeutic target areas on which to focus in the future. ..
  20. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2008
    ..abstract_text> ..
  21. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard D Kolodner; Fiscal Year: 2010
    ..cerevisiae. As a consequence, it is anticipated that these studies will provide genetic and biochemical insights that can be applied to the study of the genetics of human cancer susceptibility. ..
  22. MOLECULAR GENETICS OF TRYPANOSOME ANTIGENIC VARIATION
    GEORGE ALAN MARTIN CROSS; Fiscal Year: 2010
    ..abstract_text> ..
  23. FORMATION AND RESOLUTION OF RECOMBINATION INTERMEDIATES
    Patrick Sung; Fiscal Year: 2010
    ..For these reasons, the studies outlined in the renewal application have direct relevance to cancer biology and public health. ..
  24. ENZYMATIC MECHANISMS OF GENETIC RECOMBINATION
    Richard D Kolodner; Fiscal Year: 2010
    ....
  25. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard D Kolodner; Fiscal Year: 2011
    ..These insights will lead to new tools for cancer diagnostics as well as insights for use in improving the efficacy of known chemotherapeutic agents as well as for use in the development of new therapeutic approaches. ..
  26. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard Kolodner; Fiscal Year: 2001
    ..In addition, it is anticipated that these studies will provide tools for use in the analysis of mismatch repair in higher eukaryotes. ..
  27. BLM-mediated Homologous Recombination Regulation and Tumor Suppression
    Patrick Sung; Fiscal Year: 2008
    ..BLM also associates with a novel polypeptide BLAP75 and binds the hRad51 recombinase...
  28. ENZYMATIC MECHANISMS OF GENETIC RECOMBINATION
    Richard Kolodner; Fiscal Year: 2008
    ..The ultimate goal of these studies will be to provide a comprehensive picture of the pathways and mechanisms that suppress genome instability. ..
  29. MOLECULAR GENETICS OF TRYPANOSOME ANTIGENIC VARIATION
    GEORGE CROSS; Fiscal Year: 2008
    ....
  30. Role of Rad51 Paralogs in Mammalian DNA Repair
    Patrick Sung; Fiscal Year: 2004
    ..The studies should shed some light on the biological functions of the Rad51 paralogs and contribute to deciphering the mechanism of homologous recombinational repair in higher eukaryotic cells. ..
  31. ENZYMATIC MECHANISMS OF GENETIC RECOMBINATION
    Richard Kolodner; Fiscal Year: 2004
    ..The ultimate goal of these studies will be to provide a comprehensive picture of the pathways and mechanisms that suppress genome instability. ..
  32. ENZYMATIC MECHANISMS OF GENETIC RECOMBINATION
    Richard Kolodner; Fiscal Year: 2003
    ..The ultimate goal of these studies will be to provide a comprehensive picture of the pathways and mechanisms that suppress genome instability. ..
  33. Telomere structure and function in Trypanosoma brucei
    GEORGE CROSS; Fiscal Year: 2003
    ..There are significant similarities between the telomeres of trypanosomes and humans, so trypanosomes would be a useful model in which to study events that are relevant to human aging and cancer. ..
  34. YEAST DNA REPAIR GENES AND PROTEINS OF THE RAD52 GROUP
    Patrick Sung; Fiscal Year: 2002
    ..The results from these studies will be important for understanding the mechanism of DNA double-strand break re air b recombination in eukaryotes including humans. ..
  35. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard Kolodner; Fiscal Year: 2002
    ..In addition, it is anticipated that these studies will provide tools for use in the analysis of mismatch repair in higher eukaryotes. ..
  36. YEAST DNA REPAIR GENES AND PROTEINS OF THE RAD52 GROUP
    Patrick Sung; Fiscal Year: 2000
    ..The results from these studies will be important for understanding the mechanism of DNA double-strand break re air b recombination in eukaryotes including humans. ..
  37. FORMATION AND PROCESSING OF RECOMBINATION INTERMEDIATES
    Patrick Sung; Fiscal Year: 1999
    ..The Msh4 and Msh5 proteins will be purified and biochemical studies will be carried out to test the hypothesis that they interact with the Holliday structure and with other recombination intermediates. ..
  38. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard Kolodner; Fiscal Year: 1999
    ..It is also anticipated that these studies will provide tools for identifying additional MMR genes in higher eukaryotes for use in studying MMR and the genetics of human cancer susceptibility. ..
  39. YEAST DNA REPAIR GENES AND PROTEINS OF THE RAD52 GROUP
    Patrick Sung; Fiscal Year: 2004
    ..The results from these studies will be important for understanding the mechanism of DNA double-strand break re air b recombination in eukaryotes including humans. ..
  40. Telomere structure and function in Trypanosoma brucei
    GEORGE CROSS; Fiscal Year: 2005
    ..There are significant similarities between the telomeres of trypanosomes and humans, so trypanosomes would be a useful model in which to study events that are relevant to human aging and cancer. ..
  41. MOLECULAR GENETICS OF TRYPANOSOME ANTIGENIC VARIATION
    GEORGE CROSS; Fiscal Year: 2005
    ..We will also explore the possibility of developing 'forward' genetic approaches that will be essential for identifying novel genes that are undoubtedly involved in ES silencing and switching. ..
  42. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard Kolodner; Fiscal Year: 2008
    ..cerevisiae. As a consequence, it is anticipated that these studies will provide genetic and biochemical insights that can be applied to the study of the genetics of human cancer susceptibility. ..
  43. Molecular Basis of BRCA2-mediated DNA Repair and Cancer Avoidance
    Patrick Sung; Fiscal Year: 2008
    ..Our studies are therefore highly germane for cancer etiology, as the molecular information derived from them can be exploited to advance cancer diagnosis, prognosis and treatment. ..
  44. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2007
    ..abstract_text> ..
  45. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard Kolodner; Fiscal Year: 2007
    ..cerevisiae. As a consequence, it is anticipated that these studies will provide genetic and biochemical insights that can be applied to the study of the genetics of human cancer susceptibility. [unreadable] [unreadable] [unreadable]..
  46. Homology-directed Repair of DNA Breaks in Humans
    Patrick Sung; Fiscal Year: 2006
    ..The results should shed some light on the mechanistic underpinnings of the homologous recombination machinery in human cells and will have important implications for cancer etiology and prevention. ..
  47. Role of Rad51 Paralogs in Mammalian DNA Repair
    Patrick Sung; Fiscal Year: 2006
    ..The studies should shed some light on the biological functions of the Rad51 paralogs and contribute to deciphering the mechanism of homologous recombinational repair in higher eukaryotic cells. ..
  48. Homology-directed Repair of DNA Breaks in Humans
    Patrick Sung; Fiscal Year: 2005
    ..The results should shed some light on the mechanistic underpinnings of the homologous recombination machinery in human cells and will have important implications for cancer etiology and prevention. ..
  49. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2005
    ..abstract_text> ..
  50. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2006
    ..abstract_text> ..
  51. Molecular Analysis of Del(9q) Acute Myeloid Leukemia
    DAVID SWEETSER; Fiscal Year: 2007
    ..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. [unreadable] [unreadable] [unreadable]..
  52. Molecular Analysis of Del(9q) Acute Myeloid Leukemia
    David A Sweetser; Fiscal Year: 2010
    ..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. ..
  53. Molecular Analysis of Del(9q) Acute Myeloid Leukemia
    DAVID SWEETSER; Fiscal Year: 2009
    ..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. ..
  54. Molecular Analysis of Del(9q) Acute Myeloid Leukemia
    DAVID SWEETSER; Fiscal Year: 2006
    ..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. [unreadable] [unreadable] [unreadable]..
  55. Molecular Analysis of Del(9q) Acute Myeloid Leukemia
    DAVID SWEETSER; Fiscal Year: 2008
    ..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. ..