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Genomes and Genes | BLAP75SummaryGene Symbol: BLAP75 Description: RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) Alias: BLAP75, C9orf76, FAAP75, RP11-346I8.1, BLM-associated polypeptide, 75 kDa, BLM-associated protein 75 kDa, homolog of yeast RecQ-mediated genome instability 1 (RMI1), recQ-mediated genome instability protein 1 Species: human Top Publications
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BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrityJinhu Yin
Laboratory of Genetics, National Institute on Aging, National Institute of Health, Baltimore, MD 21224, USA
EMBO J 24:1465-76. 2005..Here we demonstrate that BLAP75, a novel protein containing a putative OB-fold nucleic acid binding domain, is an integral component of BLM ..- BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasomeThiyam Ramsing Singh
Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
Genes Dev 22:2856-68. 2008..BLM encodes a DNA helicase of the RECQ family, and associates with Topo IIIalpha and BLAP75/RMI1 (BLAP for BLM-associated polypeptide/RecQ-mediated genome instability) to form the BTB (BLM-Topo IIIalpha-.. 
Genetic variant of the human homologous recombination-associated gene RMI1 (S455N) impacts the risk of AML/MDS and malignant melanomaKarin Broberg
Department of Occupational and Environmental Medicine, Lund University Hospital, SE 221 85, Lund, Sweden
Cancer Lett 258:38-44. 2007The newly identified protein BLAP75/RMI1 associates with the helicase BLM and is critical for the function of the homologous recombination complex...- Structure and cellular roles of the RMI core complex from the bloom syndrome dissolvasomeKelly A Hoadley
Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, 550 Medical Science Center, 1300 University Avenue, Madison, WI 53706 1532, USA
Structure 18:1149-58. 2010..The RMI core interface is therefore crucial for BLM dissolvasome assembly and may have additional cellular roles as a docking hub for other proteins... - DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2Petr Cejka
Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
Nature 467:112-6. 2010..We suggest that Top3-Rmi1 and MRX are important for recruitment of the Sgs1-Dna2 complex to DSBs. Our experiments provide a mechanistic framework for understanding the initial steps of recombinational DNA repair in eukaryotes... - Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiaeHengyao Niu
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Nature 467:108-11. 2010..Our reconstituted system should serve as a useful model for delineating the mechanistic intricacy of the DNA break resection process in eukaryotes... 
Developing T lymphocytes are uniquely sensitive to a lack of topoisomerase III alphaMaren Mönnich
Department of Developmental Immunology, Max Planck Institute of Immunobiology, Freiburg, Germany
Eur J Immunol 40:2379-84. 2010..Deficiency in top3a activates a p53-dependent check-point but does not affect VDJ recombination. Our results suggest that TOP3A could be a candidate gene involved in human primary immunodeficiency syndromes...- Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1Jay Yang
Department of Biochemistry and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada and
J Biol Chem 285:21426-36. 2010.... - An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologsChi Fu Chen
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ, USA
EMBO J 29:1713-25. 2010..This work identifies SE as a new molecular function of BLM/Sgs1, and we propose that at least one role of SE is to mediate the strand-passage events catalysed by Top3-Rmi1... - MHF1-MHF2, a histone-fold-containing protein complex, participates in the Fanconi anemia pathway via FANCMThiyam Ramsing Singh
Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation and University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
Mol Cell 37:879-86. 2010..These findings reveal critical roles of the MHF1-MHF2 dimer in DNA damage repair and genome maintenance through FANCM... - A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stabilityZhijiang Yan
Laboratory of Genetics, National Institute of Aging, National Institutes of Health, Baltimore, MD 21224, USA
Mol Cell 37:865-78. 2010..Thus, FANCM-MHF is an essential DNA-remodeling complex that protects replication forks from yeast to human... - FANCM connects the genome instability disorders Bloom's Syndrome and Fanconi AnemiaAndrew J Deans
London Research Institute, Cancer Research UK, South Mimms, UK
Mol Cell 36:943-53. 2009..Moreover, a common phenotype of BS and FA cells-an elevated frequency of sister chromatid exchanges-was due to a loss of interaction of the two complexes through FANCM... - RMI1 deficiency in mice protects from diet and genetic-induced obesityAkira Suwa
Department of Metabolic Diseases, Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc, Tsukuba, Ibaraki, Japan
FEBS J 277:677-86. 2010..Taken together, these findings demonstrate that RMI1 is a novel molecule that regulates energy homeostasis... - Identification of Trypanosoma brucei RMI1/BLAP75 homologue and its roles in antigenic variationHee Sook Kim
Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York, United States of America
PLoS ONE 6:e25313. 2011.... - Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complexFeng Wang
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Structure 18:1159-70. 2010..We also revealed the structural basis of the interaction between RMI1C-OB and RMI2-OB and demonstrated the functional importance of the RMI1-RMI2 interaction in genome stability maintenance... - Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaksEun Yong Shim
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
EMBO J 29:3370-80. 2010..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation... - Processing of homologous recombination repair intermediates by the Sgs1-Top3-Rmi1 and Mus81-Mms4 complexesIan D Hickson
Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark
Cell Cycle 10:3078-85. 2011..In this review, we discuss the implications of these results and review the putative roles for the Sgs1-Top3-Rmi1 and Mus81-Mms4 complexes in the processing of various types of HRR intermediates during S phase... - Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylationXuefeng Chen
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
Nat Struct Mol Biol 18:1015-9. 2011..Poorly recruited dna2T4A S17A S237A and dna2ΔN248 mutant proteins promote resection only in the presence of Exo1, suggesting cross-talk between Dna2- and Exo1-dependent resection pathways... - Glucose regulates RMI1 expression through the E2F pathways in adipose cellsAkira Suwa
Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc, 21 Miyukigaoka, Tsukuba shi, Ibaraki, 305 8585, Japan
Endocrine 40:56-61. 2011..Further, knockdown of either E2F5 or E2F8 mRNA using siRNA suppressed this glucose-induced up-regulation of RMI1 expression. These results suggest that RMI1 expression may be regulated by glucose, at least in part, via E2F expression... - Aberrant chromosome morphology in human cells defective for Holliday junction resolutionThomas Wechsler
London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
Nature 471:642-6. 2011.... - Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damageHocine W Mankouri
Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
Proc Natl Acad Sci U S A 108:4944-9. 2011..We propose that HJ resolvases and Sgs1-Top3-Rmi1 comprise two independent processes to deal with HJ-containing DNA intermediates arising during HRR in S-phase... - Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiaeThomas M Ashton
Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute 18 1, University of Copenhagen, DK 2200 Copenhagen, Denmark
Mol Cell Biol 31:1921-33. 2011..We propose that the Sgs1-Top3-Rmi1 complex constitutes the main pathway for the processing of HJ-containing HRR intermediates but that Mus81-Mms4 can also resolve these intermediates... - RMI1 attenuates tumor development and is essential for early embryonic survivalH Chen
Department of Experimental Radiation Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
Mol Carcinog 50:80-8. 2011RMI1/BLAP75 (RecQ-mediated genome instability 1/Bloom-associated protein 75) is an OB-fold protein highly conserved from yeast to human... - DNA end resection--unraveling the tailEleni P Mimitou
Department of Microbiology and Immunology, Columbia University Medical Center, 701 W 168th St, New York, NY 10032, USA
DNA Repair (Amst) 10:344-8. 2011..Here we review recent in vitro and in vivo findings that shed more light into the mechanisms of DSB processing in mitotic and meiotic DSB repair as well as in telomere metabolism... - Adipocyte hyperplasia and RMI1 in the treatment of obesityAkira Suwa
Drug Discovery Research, Astellas Pharma Inc, Ibaraki, Japan
FEBS J 278:565-9. 2011.... - Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3Petr Cejka
Department of Microbiology, University of California, Davis, Davis, California, USA
Nat Struct Mol Biol 17:1377-82. 2010..Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity... - Colorectal cancer and polymorphisms in DNA repair genes WRN, RMI1 and BLMBernd Frank
Division of Clinical Epidemiology and Aging Research, German Cancer Research Center, 69115 Heidelberg, Germany
Carcinogenesis 31:442-5. 2010..We investigated associations of WRN, BLM and BLM-associated protein (BLAP75/RMI1) gene polymorphisms and risk of colorectal cancer (CRC), genotyping WRN V114I (rs2230009), WRN L1074F (.. - Predisposition for TMPRSS2-ERG fusion in prostate cancer by variants in DNA repair genesManuel Luedeke
1Institute of Human Genetics, University Hospital Ulm, Ulm, Germany
Cancer Epidemiol Biomarkers Prev 18:3030-5. 2009..02; OR, 1.33; 95% CI, 1.04-1.70) in unselected PCa cases. The DNA repair genes POLI and ESCO1 are proposed as susceptibility genes for TMPRSS2-ERG fusion-positive PCa that warrant further investigation... - Dissolution of double Holliday junctions by the concerted action of BLM and topoisomerase IIIalphaCsanad Z Bachrati
Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
Methods Mol Biol 582:91-102. 2009..In this chapter, we provide a detailed experimental protocol for the preparation of an oligonucleotide-based, double Holliday junction substrate and for the biochemical analysis of dissolution in vitro... - Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3Hocine W Mankouri
Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, United Kingdom
Mol Biol Cell 18:4062-73. 2007..We propose a model in which the Shu proteins act in HRR to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex... - BLM is required for faithful chromosome segregation and its localization defines a class of ultrafine anaphase bridgesKok Lung Chan
Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
EMBO J 26:3397-409. 2007..localizes to anaphase bridges, where it colocalizes with its cellular partners, topoisomerase IIIalpha and hRMI1 (BLAP75)... - Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesionMong Sing Lai
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai, Miyagi 980 8578, Japan
EMBO Rep 8:685-90. 2007..Loss of RAD51 also suppresses the cohesion defect of rmi1 mutant cells. These results indicate the existence of a new pathway involving Rad51 and Sgs1-Top3-Rmi1, which leads to the establishment of sister chromatid cohesion... - Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCMAlberto Ciccia
Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
Mol Cell 25:331-43. 2007..Our data indicate that the FANCM/FAAP24 complex may play a key role in recruitment of the FA core complex to damaged DNA... - Sgs1 regulates gene conversion tract lengths and crossovers independently of its helicase activityYi Chen Lo
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
Mol Cell Biol 26:4086-94. 2006..We propose that Sgs1 has helicase-dependent functions in replication and helicase-independent functions in DSB repair by HR... - A double Holliday junction dissolvasome comprising BLM, topoisomerase IIIalpha, and BLAP75Steven Raynard
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 281:13861-4. 2006..cellular protein complexes, all of which contain topoisomerase IIIalpha (Topo IIIalpha) and a novel protein BLAP75. Here, using highly purified human proteins, we show that BLAP75 associates independently with both Topo IIIalpha .. - BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediatesLeonard Wu
Cancer Research UK, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
Proc Natl Acad Sci U S A 103:4068-73. 2006..Here we show that a recently identified third component of the human BLM/hTOPO IIIalpha complex, BLAP75/RMI1, promotes dissolution catalyzed by hTOPO IIIalpha... - Delineation of the minimal commonly deleted segment and identification of candidate tumor-suppressor genes in del(9q) acute myeloid leukemiaDavid A Sweetser
Department of Pediatrics, Massachusetts General Hospital, 55 Fruit Street Jackson 904, Boston, MA 02114, USA
Genes Chromosomes Cancer 44:279-91. 2005..genes, FRMD3, UBQLN1, GKAP42, KIF27, HNRPK, SLC28A3, and NTRK2, and 4 novel genes, RASEF, C9orf103, C9orf64, and C9orf76. In addition, TLE1 and TLE4 are adjacent to the CDR... - Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complexJanet R Mullen
Department of Molecular Biology and Biochemistry, Rutgers University, CABM, 679 Hoes Ln, Piscataway, NJ 08854, USA
Mol Cell Biol 25:4476-87. 2005..We propose that the DNA binding specificity of Rmi1 plays a role in targeting Sgs1-Top3 to appropriate substrates... - RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complexMichael Chang
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
EMBO J 24:2024-33. 2005..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response... - Binding and activation of DNA topoisomerase III by the Rmi1 subunitChi Fu Chen
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA
J Biol Chem 282:28971-9. 2007..These results demonstrate that Top3-Rmi1 functions as a complex and suggest that Rmi1 stimulates Top3 by promoting its interaction with ssDNA... - Holliday junction processing activity of the BLM-Topo IIIalpha-BLAP75 complexWendy Bussen
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 282:31484-92. 2007..BLM is stably associated with topoisomerase IIIalpha (Topo IIIalpha) and the BLAP75 protein... - Specific pathways prevent duplication-mediated genome rearrangementsChristopher D Putnam
Ludwig Institute for Cancer Research, Department of Medicine, University of California School of Medicine, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0669, USA
Nature 460:984-9. 2009..This explains how extensive genome instability is prevented in eukaryotic cells whose genomes contain numerous divergent repeated sequences... - Association between polymorphisms in RMI1, TOP3A, and BLM and risk of cancer, a case-control studyKarin Broberg
Department of Occupational and Environmental Medicine, Lund University, Lund, Sweden
BMC Cancer 9:140. 2009..Mutations altering BLM function are associated with highly elevated cancer susceptibility (Bloom syndrome). Thus, genetic variants of BLM and proteins that form complexes with BLM, such as TOP3A and RMI1, might affect cancer risk as well... - Sgs1 function in the repair of DNA replication intermediates is separable from its role in homologous recombinational repairKara A Bernstein
Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA
EMBO J 28:915-25. 2009..Taken together, these results suggest that the Sgs1 function in repair of DNA replication intermediates can be uncoupled from its role in homologous recombinational repair... - Topoisomerase 3alpha and RMI1 suppress somatic crossovers and are essential for resolution of meiotic recombination intermediates in Arabidopsis thalianaFrank Hartung
Botany II, University of Karlsruhe, Karlsruhe, Germany
PLoS Genet 4:e1000285. 2008..that Topoisomerase 3alpha (TOP3alpha) functions together with a RecQ DNA helicase and a third partner, RMI1/BLAP75, in the resolution step of homologous recombination in a process called Holliday Junction dissolution in .. - The Arabidopsis BLAP75/Rmi1 homologue plays crucial roles in meiotic double-strand break repairLiudmila Chelysheva
INRA de Versailles, Institut Jean Pierre Bourgin, Station de Génétique et d Amélioration des Plantes UR 254, Versailles, France
PLoS Genet 4:e1000309. 2008In human cells and in Saccharomyces cerevisiae, BLAP75/Rmi1 acts together with BLM/Sgs1 and TopoIIIalpha/Top3 to maintain genome stability by limiting crossover (CO) formation in favour of NCO events, probably through the dissolution of .. - RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stabilityDongyi Xu
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, Baltimore, Maryland 21224, USA
Genes Dev 22:2843-55. 2008..Our data suggest that multi-OB-fold complexes mediate two modes of BLM action: via RPA-mediated protein-DNA interaction, and via RMI-mediated protein-protein interactions... - More complexity to the Bloom's syndrome complexYilun Liu
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Genes Dev 22:2737-42. 2008..RMI2 may be a representative of a new family of OB-fold-containing proteins that are important for complex stabilization and checkpoint response... - Functional role of BLAP75 in BLM-topoisomerase IIIalpha-dependent holliday junction processingSteven Raynard
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 283:15701-8. 2008The BLAP75 protein combines with the BLM helicase and topoisomerase (Topo) IIIalpha to form an evolutionarily conserved complex, termed the BTB complex, that functions to regulate homologous recombination... - The RecQ helicase-topoisomerase III-Rmi1 complex: a DNA structure-specific 'dissolvasome'?Hocine W Mankouri
Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
Trends Biochem Sci 32:538-46. 2007.... - A novel ubiquitin ligase is deficient in Fanconi anemiaAmom Ruhikanta Meetei
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
Nat Genet 35:165-70. 2003..Our data suggest that PHF9 has a crucial role in the Fanconi anemia pathway as the likely catalytic subunit required for monoubiquitination of FANCD2...
Research Grants
- Genomic Stability and RecQ DNA Helicases in YeastSteven J Brill; Fiscal Year: 2010..Thus, this research will provide new understanding about the factors and genetic pathways that maintain genome stability in normal and BS cells. ..
- Role of BLM protein complex in the maintenance of genome integrityValeria Busygina; Fiscal Year: 2007..ours have found that BLM works in conjunction with topoisomerase Ilia (Topo Ilia) and the newly identified protein BLAP75 to mediate the dissolution of the double Holliday junction (DHJ) to form exclusively non-crossover recombinants...
- BLM-mediated Homologous Recombination Regulation and Tumor SuppressionPatrick Sung; Fiscal Year: 2007..BLM also associates with a novel polypeptide BLAP75 and binds the hRad51 recombinase...
- BLM-mediated Homologous Recombination Regulation and Tumor SuppressionPatrick Sung; Fiscal Year: 2010..BLM also associates with a novel polypeptide BLAP75 and binds the hRad51 recombinase...
- Molecular Analysis of Del(9q) Acute Myeloid LeukemiaDavid A Sweetser; Fiscal Year: 2010..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. ..
- Molecular Analysis of Del(9q) Acute Myeloid LeukemiaDAVID SWEETSER; Fiscal Year: 2007..The information from this study will hopefully aid in the rational design of effective targeted therapies and may have relevance to the pathogenesis of other cancers. ..