Gene Symbol: ATP8A1
Description: ATPase phospholipid transporting 8A1
Alias: ATPASEII, ATPIA, ATPP2, phospholipid-transporting ATPase IA, ATPase II, ATPase class I type 8A member 1, ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1, P4-ATPase flippase complex alpha subunit ATP8A1, aminophospholipid translocase, chromaffin granule ATPase II
Species: human
Products:     ATP8A1

Top Publications

  1. Kato U, Inadome H, Yamamoto M, Emoto K, Kobayashi T, Umeda M. Role for phospholipid flippase complex of ATP8A1 and CDC50A proteins in cell migration. J Biol Chem. 2013;288:4922-34 pubmed publisher
    ..We demonstrated that CDC50A associated with and recruited P4-ATPase ATP8A1 to the plasma membrane...
  2. Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, et al. ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent manner. J Biol Chem. 2011;286:38159-67 pubmed publisher
    ..These results indicate that ATP9B is able to exit the ER and localize to the TGN independently of CDC50 proteins and that this protein contains a Golgi localization signal in its N-terminal cytoplasmic region. ..
  3. van der Velden L, Wichers C, van Breevoort A, Coleman J, Molday R, Berger R, et al. Heteromeric interactions required for abundance and subcellular localization of human CDC50 proteins and class 1 P4-ATPases. J Biol Chem. 2010;285:40088-96 pubmed publisher
    ..ATP8B1 and ATP8B2 co-immunoprecipitated with CDC50A and CDC50B, whereas ATP8B4, ATP8A1, and ATP8A2 associated only with CDC50A...
  4. Liou A, Molday L, Wang J, Andersen J, Molday R. Identification and functional analyses of disease-associated P4-ATPase phospholipid flippase variants in red blood cells. J Biol Chem. 2019;294:6809-6821 pubmed publisher
    ..MS, we show that ATP11C is the only abundant P4-ATPase phospholipid flippase in human RBCs, whereas ATP11C and ATP8A1 are the major P4-ATPases in mouse RBCs. We also found that ATP11A and ATP11B are present at low levels...
  5. Kohara H, Utsugisawa T, Sakamoto C, Hirose L, Ogawa Y, Ogura H, et al. KLF1 mutation E325K induces cell cycle arrest in erythroid cells differentiated from congenital dyserythropoietic anemia patient-specific induced pluripotent stem cells. Exp Hematol. 2019;: pubmed publisher
    ..disulfide reductase (GSR), glucose phosphate isomerase (GPI), and ATPase phospholipid transporting 8A1 (ATP8A1)...
  6. Haas M, Shah G, Onstead Haas L, Mooradian A. Identification of ATP8B1 as a blood-brain barrier-enriched protein. Cell Mol Neurobiol. 2014;34:473-8 pubmed publisher
    ..These results suggest that ATP8B1, a P-type aminophospholipid translocase, is enriched in cerebral microvessels and may have a role in plasma membrane lipid transport.
  7. Chen Y, Klöditz K, Lee E, Nguyen D, Yuan Q, Johnson J, et al. Structure and function analysis of the C. elegans aminophospholipid translocase TAT-1. J Cell Sci. 2019;132: pubmed publisher
    The Caenorhabditis elegans aminophospholipid translocase TAT-1 maintains phosphatidylserine (PS) asymmetry in the plasma membrane and regulates endocytic transport...
  8. McGough I, de Groot R, Jellett A, Betist M, Varandas K, Danson C, et al. SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion. Nat Commun. 2018;9:3737 pubmed publisher
    ..conserved endosome-associated membrane re-modelling complex composed of MON2, DOPEY2 and the putative aminophospholipid translocase, ATP9A...
  9. Wang J, Molday L, Hii T, Coleman J, Wen T, Andersen J, et al. Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues. Sci Rep. 2018;8:10795 pubmed publisher
    ..Of the 12 P4-ATPase that associate with CDC50 subunits, 10 P4-ATPases were detected. Four P4-ATPases (ATP8A1, ATP11A, ATP11B, ATP11C) were present in all five tissues...

More Information


  1. Jia F, Qi S, Li H, Liu P, Li P, Wu C, et al. Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance. Biochem Biophys Res Commun. 2014;454:505-11 pubmed publisher
    ..In vivo and in vitro analysis showed that AtLEA14 could effectively stabilize AtPP2-B11, an important E3 ligase...
  2. Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C. SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis. J Exp Bot. 2015;66:4683-97 pubmed publisher
    ..In this study, we identified an F-box protein from Arabidopsis thaliana, AtPP2-B11, which was remarkably induced with increased duration of salt treatment in terms of both transcript and ..
  3. Pagliari L, Buoso S, Santi S, Furch A, Martini M, Degola F, et al. Filamentous sieve element proteins are able to limit phloem mass flow, but not phytoplasma spread. J Exp Bot. 2017;68:3673-3688 pubmed publisher
    ..lacking one or two genes related to sieve element filament formation-AtSEOR1 (At3g01680), AtSEOR2 (At3g01670), and AtPP2-A1 (At4g19840)-were analysed...
  4. Taye M, Kim J, Yoon S, Lee W, Hanotte O, Dessie T, et al. Whole genome scan reveals the genetic signature of African Ankole cattle breed and potential for higher quality beef. BMC Genet. 2017;18:11 pubmed publisher
    ..MB and SLC48A1 affect meat color. In addition, we identified genes (TIMP2, PKM2, PRKG1, MAP3K5, and ATP8A1) related to feeding efficiency...
  5. Yabas M, Jing W, Shafik S, Bröer S, Enders A. ATP11C Facilitates Phospholipid Translocation across the Plasma Membrane of All Leukocytes. PLoS ONE. 2016;11:e0146774 pubmed publisher
    ..These findings show that all leukocyte lineages exhibit flippase activity, and identify ATP11C as an aminophospholipid translocase in immune cells.
  6. Chaubey P, Hofstetter L, Roschitzki B, Stieger B. Proteomic Analysis of the Rat Canalicular Membrane Reveals Expression of a Complex System of P4-ATPases in Liver. PLoS ONE. 2016;11:e0158033 pubmed publisher
    ..In addition to ATP8B1, four more P4-ATPases were identified. ATP8A1 and ATP9A showed expression specific to the canalicular membrane, ATP11C at the bLPM and ATP11A in an ..
  7. Kerr D, Marsillo A, Guariglia S, Budylin T, Sadek R, Menkes S, et al. Aberrant hippocampal Atp8a1 levels are associated with altered synaptic strength, electrical activity, and autistic-like behavior. Biochim Biophys Acta. 2016;1862:1755-65 pubmed publisher
    ..A pronounced induction of the flippase Atp8a1 was observed in post-mortem tissue homogenates from the hippocampus and temporal lobe of juvenile autistic ..
  8. Halleck M, Pradhan D, Blackman C, Berkes C, Williamson P, Schlegel R. Multiple members of a third subfamily of P-type ATPases identified by genomic sequences and ESTs. Genome Res. 1998;8:354-61 pubmed
    ..The recently cloned gene for the mammalian aminophospholipid translocase belongs to this new subfamily, suggesting that other subfamily members may transport other lipids or ..
  9. Williamson P, Schlegel R. Transbilayer phospholipid movement and the clearance of apoptotic cells. Biochim Biophys Acta. 2002;1585:53-63 pubmed
    ..distribution of phospholipids across their plasma membranes, an asymmetry normally maintained by an aminophospholipid translocase. This disruption of asymmetry, mediated by an activity known as the scramblase, generates ligands on ..
  10. Kariuki S, Ghodke Puranik Y, Dorschner J, Chrabot B, Kelly J, Tsao B, et al. Genetic analysis of the pathogenic molecular sub-phenotype interferon-alpha identifies multiple novel loci involved in systemic lupus erythematosus. Genes Immun. 2015;16:15-23 pubmed publisher
  11. Lee S, Uchida Y, Wang J, Matsudaira T, Nakagawa T, Kishimoto T, et al. Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase. EMBO J. 2015;34:669-88 pubmed publisher
    ..Depletion of ATP8A1 impaired the asymmetric transbilayer distribution of PS in REs, dissociated EHD1 from REs, and generated aberrant ..
  12. Dong W, Yao C, Teng X, Chai J, Yang X, Li B. MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer. Tumour Biol. 2016;37:2973-85 pubmed publisher
    ..b>ATP8A1 was demonstrated as a novel direct target of miR-140-3p using a luciferase assay...
  13. Tyurina Y, Basova L, Konduru N, Tyurin V, Potapovich A, Cai P, et al. Nitrosative stress inhibits the aminophospholipid translocase resulting in phosphatidylserine externalization and macrophage engulfment: implications for the resolution of inflammation. J Biol Chem. 2007;282:8498-509 pubmed
    ..PS), which is normally maintained within the cytosolic leaflet of the plasma membrane by aminophospholipid translocase (APLT). APLT is sensitive to redox modifications of its -SH groups...
  14. Noji T, Yamamoto T, Saito K, Fujimura Kamada K, Kondo S, Tanaka K. Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast. Biochem Biophys Res Commun. 2006;344:323-31 pubmed
    Lem3p-Dnf1p is a putative aminophospholipid translocase (APLT) complex that is localized to the plasma membrane; Lem3p is required for Dnf1p localization to the plasma membrane...
  15. Halleck M, Lawler JF J, Blackshaw S, Gao L, Nagarajan P, Hacker C, et al. Differential expression of putative transbilayer amphipath transporters. Physiol Genomics. 1999;1:139-50 pubmed
    The aminophospholipid translocase transports phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another...
  16. Cheng C, Wang Z, Ren Z, Zhi L, Yao B, Su C, et al. SCFAtPP2-B11 modulates ABA signaling by facilitating SnRK2.3 degradation in Arabidopsis thaliana. PLoS Genet. 2017;13:e1006947 pubmed publisher
    ..3 is degradated by 26S proteasome system and ABA promotes its degradation. We found that SnRK2.3 interacts with AtPP2-B11 directly...
  17. Pomorski T, Lombardi R, Riezman H, Devaux P, van Meer G, Holthuis J. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Mol Biol Cell. 2003;14:1240-54 pubmed
    ..contains two P-type ATPases, Dnf1p and Dnf2p, with structural similarity to ATPase II, a candidate aminophospholipid translocase from bovine chromaffin granules...
  18. Baldridge R, Xu P, Graham T. Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain. J Biol Chem. 2013;288:19516-27 pubmed publisher
    ..Moreover, residues from Atp8a1, a mammalian ortholog of Drs2, in these positions allow PS recognition by Dnf1...
  19. Sokal A, Bartosz G. Uncouplers of mitochondrial oxidative phosphorylation are not substrates of the erythrocyte glutathione-S-conjugate pump. Arch Biochem Biophys. 1998;349:113-21 pubmed
    ..of these findings may indicate the electrogenic nature of MRP1-mediated transport of glutathione-S conjugates and stimulation of aminophospholipid translocase (flippase) rather than the glutathione-S-conjugate pump by the uncouplers.
  20. Harris M, Le Couteur D, Arias I. Progressive familial intrahepatic cholestasis: genetic disorders of biliary transporters. J Gastroenterol Hepatol. 2005;20:807-17 pubmed
    ..The gene codes for an aminophospholipid translocase protein that maintains the integrity of the membrane...
  21. Matsudaira T, Mukai K, Noguchi T, Hasegawa J, Hatta T, Iemura S, et al. Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells. Nat Commun. 2017;8:1246 pubmed publisher
    ..Among these proteins are YAP and multiple proteins related to YAP signalling. Knockdown of ATP8A1 (an RE PS-flippase) or evectin-2 (an RE-resident protein) and masking of PS in the cytoplasmic leaflet of ..
  22. Paulusma C, Oude Elferink R. The type 4 subfamily of P-type ATPases, putative aminophospholipid translocases with a role in human disease. Biochim Biophys Acta. 2005;1741:11-24 pubmed
    ..How the absence of an aminophospholipid translocase activity relates to this severe disease is, however, still unclear...
  23. Saito K, Fujimura Kamada K, Furuta N, Kato U, Umeda M, Tanaka K. Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:3418-32 pubmed
    ..studies suggest that CDC50 performs a function similar to DRS2, which encodes a P-type ATPase of the aminophospholipid translocase (APT) subfamily...
  24. Alvarez L, Jara P, Sánchez Sabaté E, Hierro L, Larrauri J, Díaz M, et al. Reduced hepatic expression of farnesoid X receptor in hereditary cholestasis associated to mutation in ATP8B1. Hum Mol Genet. 2004;13:2451-60 pubmed
    ..these characteristics include FIC1 disease (caused by mutations in ATP8B1, which encodes a putative aminophospholipid translocase, FIC1, whose function in bile handling is unknown) and bile salt export pump (BSEP) disease (caused by ..
  25. Mouro I, Halleck M, Schlegel R, Mattei M, Williamson P, Zachowski A, et al. Cloning, expression, and chromosomal mapping of a human ATPase II gene, member of the third subfamily of P-type ATPases and orthologous to the presumed bovine and murine aminophospholipid translocase. Biochem Biophys Res Commun. 1999;257:333-9 pubmed
    ..muscle cDNA library of a human ATPase II (h-ATPase II), orthologous to the presumed bovine and mouse aminophospholipid translocase (95.3 and 95.9% amino acid identity, respectively)...