ATOX1

Summary

Gene Symbol: ATOX1
Description: antioxidant 1 copper chaperone
Alias: ATX1, HAH1, copper transport protein ATOX1, ATX1 antioxidant protein 1 homolog, metal transport protein ATX1
Species: human
Products:     ATOX1

Top Publications

  1. Klomp L, Lin S, Yuan D, Klausner R, Culotta V, Gitlin J. Identification and functional expression of HAH1, a novel human gene involved in copper homeostasis. J Biol Chem. 1997;272:9221-6 pubmed
    ..agents and dioxygen and are auxotrophic for lysine when grown in air, and expression of this human ATX1 homologue (HAH1) in these strains restored growth on lysine-deficient media...
  2. Larin D, Mekios C, Das K, Ross B, Yang A, Gilliam T. Characterization of the interaction between the Wilson and Menkes disease proteins and the cytoplasmic copper chaperone, HAH1p. J Biol Chem. 1999;274:28497-504 pubmed
    ..interaction between the six metal-binding domains of the WD and MNK ATPases and the cytoplasmic copper chaperone HAH1. We demonstrate that several metal-binding domains interact independently or in combination with HAH1p, although ..
  3. Boultwood J, Strickson A, Jabs E, Cheng J, Fidler C, Wainscoat J. Physical mapping of the human ATX1 homologue (HAH1) to the critical region of the 5q- syndrome within 5q32, and immediately adjacent to the SPARC gene. Hum Genet. 2000;106:127-9 pubmed
    The 5q- syndrome is a myelodysplastic syndrome with the 5q deletion as the sole karyotypic abnormality. The human ATX1 homologue (HAH1), encodes a copper-binding protein with a role in antioxidant defence...
  4. Walker J, Tsivkovskii R, Lutsenko S. Metallochaperone Atox1 transfers copper to the NH2-terminal domain of the Wilson's disease protein and regulates its catalytic activity. J Biol Chem. 2002;277:27953-9 pubmed
    ..of these mutations were shown to disrupt the protein-protein interactions between WNDP and the metallochaperone Atox1, suggesting that these interactions are important for normal copper homeostasis...
  5. Hamza I, Schaefer M, Klomp L, Gitlin J. Interaction of the copper chaperone HAH1 with the Wilson disease protein is essential for copper homeostasis. Proc Natl Acad Sci U S A. 1999;96:13363-8 pubmed
    ..Previous studies in Saccharomyces cerevisiae suggested that the human copper chaperone HAH1 may play a role in copper trafficking to the secretory pathway of the cell...
  6. Wernimont A, Huffman D, Lamb A, O Halloran T, Rosenzweig A. Structural basis for copper transfer by the metallochaperone for the Menkes/Wilson disease proteins. Nat Struct Biol. 2000;7:766-71 pubmed
    The Hah1 metallochaperone protein is implicated in copper delivery to the Menkes and Wilson disease proteins...
  7. Strausak D, Howie M, Firth S, Schlicksupp A, Pipkorn R, Multhaup G, et al. Kinetic analysis of the interaction of the copper chaperone Atox1 with the metal binding sites of the Menkes protein. J Biol Chem. 2003;278:20821-7 pubmed
    ..Evidence suggests that copper is delivered to the ATPases by Atox1, one of three cytoplasmic copper chaperones...
  8. Wernimont A, Yatsunyk L, Rosenzweig A. Binding of copper(I) by the Wilson disease protein and its copper chaperone. J Biol Chem. 2004;279:12269-76 pubmed
    ..these domains are not well established, but possible functions include exchanging copper with the metallochaperone Atox1 and mediating copper-responsive cellular relocalization...
  9. Banci L, Bertini I, Ciofi Baffoni S, Chasapis C, Hadjiliadis N, Rosato A. An NMR study of the interaction between the human copper(I) chaperone and the second and fifth metal-binding domains of the Menkes protein. FEBS J. 2005;272:865-71 pubmed
    The interaction between the human copper(I) chaperone, HAH1, and one of its two physiological partners, the Menkes disease protein (ATP7A), was investigated in solution using heteronuclear NMR...

More Information

Publications125 found, 100 shown here

  1. Achila D, Banci L, Bertini I, Bunce J, Ciofi Baffoni S, Huffman D. Structure of human Wilson protein domains 5 and 6 and their interplay with domain 4 and the copper chaperone HAH1 in copper uptake. Proc Natl Acad Sci U S A. 2006;103:5729-34 pubmed
    ..An NMR titration of apoWLN5-6 with the metallochaperone Cu(I)HAH1 reveals no complex formation and no copper exchange between the two proteins, whereas titration of Cu(I)HAH1 with ..
  2. de Bie P, van De Sluis B, Burstein E, Duran K, Berger R, Duckett C, et al. Characterization of COMMD protein-protein interactions in NF-kappaB signalling. Biochem J. 2006;398:63-71 pubmed
    ..Taken together, these data support the significance of COMMD protein-protein interactions and provide new mechanistic insight into the function of this protein family in NF-kappaB signalling. ..
  3. de Bie P, van De Sluis B, Burstein E, van de Berghe P, Muller P, Berger R, et al. Distinct Wilson's disease mutations in ATP7B are associated with enhanced binding to COMMD1 and reduced stability of ATP7B. Gastroenterology. 2007;133:1316-26 pubmed
    ..This interaction was independent of intracellular copper levels and of the expression of the copper chaperone ATOX1. Four WD patient-derived mutations in this region of ATP7B significantly increased its binding to COMMD1...
  4. Banci L, Bertini I, Cantini F, Rosenzweig A, Yatsunyk L. Metal binding domains 3 and 4 of the Wilson disease protein: solution structure and interaction with the copper(I) chaperone HAH1. Biochemistry. 2008;47:7423-9 pubmed publisher
    ..Both domains can be loaded with Cu(I) when provided as an acetonitrile complex or by the chaperone HAH1. HAH1 forms a 70% complex with domain 4 that is in fast exchange with the free protein in solution...
  5. Hussain F, Olson J, Wittung Stafshede P. Conserved residues modulate copper release in human copper chaperone Atox1. Proc Natl Acad Sci U S A. 2008;105:11158-63 pubmed publisher
    It is unclear how the human copper (Cu) chaperone Atox1 delivers Cu to metal-binding domains of Wilson and Menkes disease proteins in the cytoplasm...
  6. Banci L, Bertini I, Calderone V, Della Malva N, Felli I, Neri S, et al. Copper(I)-mediated protein-protein interactions result from suboptimal interaction surfaces. Biochem J. 2009;422:37-42 pubmed publisher
    ..In the present study we address the interaction between the human copper(I)-chaperone HAH1 (human ATX1 homologue) and a metal-binding domain in one of its partners, namely the P-type copper-transporting ATPase, ATP7A (..
  7. Grasso G, Morbiducci U, Massai D, Tuszynski J, Danani A, Deriu M. Destabilizing the AXH Tetramer by Mutations: Mechanisms and Potential Antiaggregation Strategies. Biophys J. 2018;114:323-330 pubmed publisher
    ..Therefore, R638 might be also implicated in the AXH oligomerization pathway and stands out as a target for future experimental studies focused on self-association mechanisms and fibril formation of full-length ATX1.
  8. Khan Z, Mishra C, Jyotiranjan T. In silico analysis of caprine superoxide dismutase 1 (SOD1) gene. Genomics. 2019;: pubmed publisher
    ..STRING database reveals its interaction with SOD2 (Superoxide dismutase [Mn], mitochondrial), ATOX1 (Copper transport protein), RAC1 (Ras-related C3 botulinum toxin substrate 1), GPX2 (Glutathione peroxidase 2), ..
  9. Zhang S, Liu H, Amarsingh G, Cheung C, Hogl S, Narayanan U, et al. Diabetic cardiomyopathy is associated with defective myocellular copper regulation and both defects are rectified by divalent copper chelation. Cardiovasc Diabetol. 2014;13:100 pubmed publisher
    ..depressed levels of additional intracellular copper-transporting proteins, including antioxidant-protein-1 (ATOX1) and copper-transporting-ATPase-2 (ATP7B), whereas TETA elevated copper-transporting-ATPase-1 (ATP7A)...
  10. Dan Zhao -, Zhang X, Liu D, Ru S. Cu accumulation, detoxification and tolerance in the red swamp crayfish Procambarus clarkii. Ecotoxicol Environ Saf. 2019;175:201-207 pubmed publisher
    ..During Cu2+ exposure, crayfish repressed the expression level of Cu homeostasis genes (Ctr1, Atox1, copper-transporting ATPase 2, MTF-1/2, and MT) in hepatopancreas to inhibit intracellular Cu transporting...
  11. Kumari N, Kumar A, Pal A, Thapa B, Modi M, Prasad R. In-silico analysis of novel p.(Gly14Ser) variant of ATOX1 gene: plausible role in modulating ATOX1-ATP7B interaction. Mol Biol Rep. 2019;: pubmed publisher
    ..b>ATOX1, a copper chaperone that delivers copper to ATP7B, is a potential genetic modifier of WD...
  12. Ponnandai Shanmugavel K, Petranovic D, Wittung Stafshede P. Probing functional roles of Wilson disease protein (ATP7B) copper-binding domains in yeast. Metallomics. 2017;9:981-988 pubmed publisher
    After Ctr1-mediated uptake into human cells, copper (Cu) ions are transported by the cytoplasmic Cu chaperone Atox1 to the Wilson disease protein (ATP7B) in the Golgi network...
  13. Saad R, Hsouna A, Saibi W, Hamed K, Brini F, Ghneim Herrera T. A stress-associated protein, LmSAP, from the halophyte Lobularia maritima provides tolerance to heavy metals in tobacco through increased ROS scavenging and metal detoxification processes. J Plant Physiol. 2018;231:234-243 pubmed publisher
  14. Banci L, Bertini I, Cantini F, Chasapis C, Hadjiliadis N, Rosato A. A NMR study of the interaction of a three-domain construct of ATP7A with copper(I) and copper(I)-HAH1: the interplay of domains. J Biol Chem. 2005;280:38259-63 pubmed
    ..ATP7A receives copper from a soluble protein, the metallochaperone HAH1. The exact role and interplay of the six soluble domains is still quite unclear, as it has been extensively ..
  15. Kahra D, Mondol T, Niemiec M, Wittung Stafshede P. Human Copper Chaperone Atox1 Translocates to the Nucleus but does not Bind DNA In Vitro. Protein Pept Lett. 2015;22:532-8 pubmed
    After Ctr1-mediated cell uptake, copper (Cu) is transported by the cytoplasmic Cu chaperone Atox1 to P1B type ATPases ATP7A and ATP7B in the Golgi network, for incorporation into Cudependent enzymes...
  16. Li J, Zhu L, Hull J, Liang S, Daniell H, Jin S, et al. Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly). Plant Biotechnol J. 2016;14:1956-75 pubmed publisher
    ..Taken together, this study provides comprehensive insights into the cotton defense system to whitefly infestation and has identified several candidate genes for control of phloem-feeding pests. ..
  17. Lutsenko S, Bhattacharjee A, Hubbard A. Copper handling machinery of the brain. Metallomics. 2010;2:596-608 pubmed publisher
    ..homeostasis, despite the fact that the molecules mediating copper transport and distribution in the brain (CTR1, Atox1, CCS, ScoI/II, ATP7A and ATP7B) have been identified and their importance in CNS function increasingly understood...
  18. Lutsenko S, Tsivkovskii R, Walker J. Functional properties of the human copper-transporting ATPase ATP7B (the Wilson's disease protein) and regulation by metallochaperone Atox1. Ann N Y Acad Sci. 2003;986:204-11 pubmed
    ..ATPase and its regulation by copper, we have recently developed the functional expression systems for WNDP and for Atox1, a cytosolic protein that serves as an intracellular donor of copper for WNDP...
  19. Králík L, Flachsova E, Hansikova H, Saudek V, Zeman J, Martasek P. Molecular Diagnostics of Copper-Transporting Protein Mutations Allows Early Onset Individual Therapy of Menkes Disease. Folia Biol (Praha). 2017;63:165-173 pubmed
    ..The mutation c.3745G>T (p.Glu1249Ter) has not been identified previously. Molecular analysis of the ATOX1 gene as a possible modulating factor of Menkes disease did not reveal presence of pathogenic mutations...
  20. Tian Y, Fang T, Yuan S, Zheng Y, Arnesano F, Natile G, et al. Tetrathiomolybdate inhibits the reaction of cisplatin with human copper chaperone Atox1. Metallomics. 2018;10:745-750 pubmed publisher
    ..Here we found that TM can inhibit the reaction of cisplatin with Cu-Atox1 and prevent the protein unfolding and aggregation induced by cisplatin...
  21. Su D, Zhang Q, Wang X, He P, Zhu Y, Zhao J, et al. Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy. Mol Cancer Ther. 2009;8:1292-304 pubmed publisher
    ..different set of antiapoptotic (PSEN1, PPP2CA, API5, PPP2R1B, PPP2R1A, and FIS1), antioxidant (HSPD1, GSS, SOD1, ATOX1, and CAT), and proapoptotic (ENDOG, BAK1, CASP2, CASP4, PDCD5, HTRA2, SEPT4, TNFSF10, and PRODH) genes expressed ..
  22. Lenartowicz M, Kennedy C, Hayes H, McArdle H. Transcriptional regulation of copper metabolism genes in the liver of fetal and neonatal control and iron-deficient rats. Biometals. 2015;28:51-9 pubmed publisher
    ..There was no difference in gene expression between control and iron deficient animals. Atox1 expression remained approximately stable until term, and then there was a rise to a maximum at about Day 8...
  23. Hasselbalch H, Thomassen M, Riley C, Kjær L, Larsen T, Jensen M, et al. Whole blood transcriptional profiling reveals deregulation of oxidative and antioxidative defence genes in myelofibrosis and related neoplasms. Potential implications of downregulation of Nrf2 for genomic instability and disease progression. PLoS ONE. 2014;9:e112786 pubmed publisher
    ..Among the twenty most up- and downregulated genes, ATOX1, DEFB122, GPX8, PRDX2, PRDX6, PTGS1, and SEPP1 were progressively upregulated from ET over PV to PMF, whereas ..
  24. Levy A, Nissim M, Mendelman N, Chill J, Ruthstein S. Ctr1 Intracellular Loop Is Involved in the Copper Transfer Mechanism to the Atox1 Metallochaperone. J Phys Chem B. 2016;120:12334-12345 pubmed
    ..the cellular concentration and distribution of copper involves the copper transporter, Ctr1; the metallochaperone, Atox1; and the ATP7B transporter...
  25. Ndamukong I, Jones D, Lapko H, Divecha N, Avramova Z. Phosphatidylinositol 5-phosphate links dehydration stress to the activity of ARABIDOPSIS TRITHORAX-LIKE factor ATX1. PLoS ONE. 2010;5:e13396 pubmed publisher
    ..The plant trithorax factor (ATX1) tri-methylates the lysine 4 residue of histone H3 (H3K4me3) at gene coding sequences, which positively correlates ..
  26. Derntl C, Kiesenhofer D, Mach R, Mach Aigner A. Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering. Appl Environ Microbiol. 2015;81:6314-23 pubmed publisher
    ..we tested the applicability of the genes asl1 (encoding an enzyme of the l-arginine biosynthesis pathway), the hah1 (encoding an enzyme of the l-lysine biosynthesis pathway), and the pyr4 (encoding an enzyme of the uridine ..
  27. Zetzsche A, Schunter N, Zentek J, Pieper R. Accumulation of copper in the kidney of pigs fed high dietary zinc is due to metallothionein expression with minor effects on genes involved in copper metabolism. J Trace Elem Med Biol. 2016;35:1-6 pubmed publisher
    ..Kidney mRNA expression of Zn transporter ZnT1 and ZIP4, genes involved in Cu metabolism (Ctr1, Atox1, SOD1, ATP7A, CCS, CP) and divalent metal ion transport (DMT1) and binding (MT-1a, MT-2b, MT-3) were determined...
  28. Palm M, Weise C, Lundin C, Wingsle G, Nygren Y, Björn E, et al. Cisplatin binds human copper chaperone Atox1 and promotes unfolding in vitro. Proc Natl Acad Sci U S A. 2011;108:6951-6 pubmed publisher
    ..Normally, the Cu chaperone Atox1 binds Cu(I) via two cysteines and delivers the metal to metal-binding domains of ATP7B; the ATP7B domains then ..
  29. Dong Q, Kuefner M, Deng X, Bridges D, Park E, Elam M, et al. Sex-specific differences in hepatic steatosis in obese spontaneously hypertensive (SHROB) rats. Biol Sex Differ. 2018;9:40 pubmed publisher
    ..The livers of male rats also elicited higher induction of Pparg, Ppara, Slc2a4, Atox1, Skp1, Angptl3, and Pnpla3 mRNAs...
  30. Su B, Shang M, Li C, Perera D, Pinkert C, Irwin M, et al. Effects of transgenic sterilization constructs and their repressor compounds on hatch, developmental rate and early survival of electroporated channel catfish embryos and fry. Transgenic Res. 2015;24:333-52 pubmed publisher
    ..This fry output should be considered to ensure that sufficient numbers of transgenic fish are produced for future applications and for defining repressor systems that are the most successful. ..
  31. Cai H, Peng F. Knockdown of copper chaperone antioxidant-1 by RNA interference inhibits copper-stimulated proliferation of non-small cell lung carcinoma cells. Oncol Rep. 2013;30:269-75 pubmed publisher
    ..Cellular copper metabolism is regulated by a network of copper transporters and chaperones. Antioxidant-1 (ATOX1) is a cytosolic copper chaperone important for intracellular copper transport, which plays a role in the ..
  32. Wilson R, Golub S, Rowley L, Angelucci C, Karpievitch Y, Bateman J, et al. Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation. J Proteome Res. 2016;15:1033-50 pubmed publisher
    ..in glutathione biosynthesis and regeneration (Gstp1, Gsto1, and Gsr), and oxidative stress proteins (Prdx2, Txn, Atox1, Hmox1, and Vnn1)...
  33. Li W, Lacey R, Ye Y, Lu J, Yeh K, Xiao Y, et al. Triplin, a small molecule, reveals copper ion transport in ethylene signaling from ATX1 to RAN1. PLoS Genet. 2017;13:e1006703 pubmed publisher
    ..We further showed that mutants of ANTIOXIDANT PROTEIN1 (ATX1) are hypersensitive to tiplin, but with less sensitivity comparing with the ones of ran1-1 and ran1-2...
  34. Mercer S, Burke R. Evidence for a role for the putative Drosophila hGRX1 orthologue in copper homeostasis. Biometals. 2016;29:705-13 pubmed publisher
    ..role in copper homeostasis by regulating the redox activity of the metalated sites of copper chaperones such as ATOX1 and SOD1, and the copper efflux proteins ATP7A and ATP7B...
  35. Soto Arredondo K, Robles J, Díaz Cervantes E, Ruiz Ramírez C, García Revilla M, Wrobel K, et al. Effects of lead and lead-melatonin exposure on protein and gene expression of metal transporters, proteins and the copper/zinc ratio in rats. Biometals. 2018;31:859-871 pubmed publisher
    ..These data further elucidate the effects of lead on Cu and Zn and the molecular mechanism underlying lead bio-distribution in animals. ..
  36. Wu X, Gao L, Zhou K, Li X, Lin X, Wan D, et al. Deposition and transport of trace mineral elements were affected by stocking density in fattening pigs. J Trace Elem Med Biol. 2018;50:566-571 pubmed publisher
    ..05). Alternatively, ATX1 expression in the jejunal mucosa of the medium-density group, SLC30A9 in the duodenal and jejunal mucosa, ATX1 in ..
  37. Xing L, Liu Y, Xu S, Xiao J, Wang B, Deng H, et al. Arabidopsis O-GlcNAc transferase SEC activates histone methyltransferase ATX1 to regulate flowering. EMBO J. 2018;: pubmed publisher
    ..SEC activates ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1), a histone lysine methyltransferase (HKMT), through O-GlcNAc modification to augment ATX1-mediated H3K4me3 ..
  38. Napsucialy Mendivil S, Alvarez Venegas R, Shishkova S, Dubrovsky J. Arabidopsis homolog of trithorax1 (ATX1) is required for cell production, patterning, and morphogenesis in root development. J Exp Bot. 2014;65:6373-84 pubmed publisher
    Arabidopsis homolog of trithorax1 (ATX1/SDG27), a known regulator of flower development, encodes a H3K4histone methyltransferase that maintains a number of genes in an active state...
  39. Mondol T, Adén J, Wittung Stafshede P. Copper binding triggers compaction in N-terminal tail of human copper pump ATP7B. Biochem Biophys Res Commun. 2016;470:663-669 pubmed publisher
    ..the multi-domain protein ATP7B in the Golgi network receives copper from the cytoplasmic copper chaperone Atox1 and, with energy from ATP hydrolysis, moves the metal to the lumen for loading of copper-dependent enzymes...
  40. Ahn E, Kim D, Shin M, Ryu E, Yong J, Chung S, et al. Tat-ATOX1 inhibits streptozotocin-induced cell death in pancreatic RINm5F cells and attenuates diabetes in a mouse model. Int J Mol Med. 2016;38:217-24 pubmed publisher
    Antioxidant 1 (ATOX1) functions as an antioxidant against hydrogen peroxide and superoxide, and therefore may play a significant role in many human diseases, including diabetes mellitus (DM)...
  41. Qasim M, Baohua W, Zou H, Lin Y, Dash C, Bamisile B, et al. Phylogenetic relationship and genetic diversity of citrus psyllid populations from China and Pakistan and their associated Candidatus bacterium. Mol Phylogenet Evol. 2018;126:173-180 pubmed publisher
    ..Pakistan), sequence data of three different genes, cytochrome oxidase subunit I (COI), Cu-transporting protein (ATOX1) and 16S rRNA, were used to characterize all populations...
  42. Zhao H, Yang H, Yan L, Jiang S, Xue L, Zhao H, et al. [Effects of lead exposure on copper and copper transporters in choroid plexus of rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014;32:819-22 pubmed
    ..to measure the expression of copper transporters including copper transporter 1 (Ctr1), antioxidant protein 1 (ATX1), and Cu ATPase (ATP7A)...
  43. Su B, Shang M, Grewe P, Patil J, Peatman E, Perera D, et al. Suppression and restoration of primordial germ cell marker gene expression in channel catfish, Ictalurus punctatus, using knockdown constructs regulated by copper transport protein gene promoters: Potential for reversible transgenic sterilization. Theriogenology. 2015;84:1499-512 pubmed publisher
    ..Optimization of this system could allow environmentally safe application of transgenic technology and might be applicable to other applications for aquatic organisms. ..
  44. Wu X, Yuan S, Wang E, Tong Y, Ma G, Wei K, et al. Platinum transfer from hCTR1 to Atox1 is dependent on the type of platinum complex. Metallomics. 2017;9:546-555 pubmed publisher
    ..the reaction of the platinated C-terminal metal binding motif of hCTR1 (C8) with the down-stream protein Atox1. Results show that Atox1 is highly reactive to the platinated C8 adducts of cisplatin and transplatin, whereas the ..
  45. Flores A, Unger V. Atox1 contains positive residues that mediate membrane association and aid subsequent copper loading. J Membr Biol. 2013;246:903-13 pubmed publisher
    ..Here, we demonstrate that antioxidant protein 1 (Atox1 in human cells), the principal cellular copper chaperone responsible for delivery of copper to the secretory ..
  46. Kim S, Hwang I, Yoo D, Eum W, Kim D, Shin M, et al. Tat-antioxidant 1 protects against stress-induced hippocampal HT-22 cells death and attenuate ischaemic insult in animal model. J Cell Mol Med. 2015;19:1333-45 pubmed publisher
    ..Oxidative stress-induced reactive oxygen species (ROS) are responsible for various neuronal diseases. Antioxidant 1 (Atox1) regulates copper homoeostasis and promotes cellular antioxidant defence against toxins generated by ROS...
  47. Luchinat E, Secci E, Cencetti F, Bruni P. Sequential protein expression and selective labeling for in-cell NMR in human cells. Biochim Biophys Acta. 2016;1860:527-33 pubmed publisher
    ..We established a human cell line stably overexpressing the copper binding protein HAH1. A second protein (human superoxide dismutase 1, SOD1) was overexpressed by transient transfection and isotopically ..
  48. Xi Z, Guo W, Tian C, Wang F, Liu Y. Copper binding modulates the platination of human copper chaperone Atox1 by antitumor trans-platinum complexes. Metallomics. 2014;6:491-7 pubmed publisher
    ..The copper chaperone Atox1 has been shown to bind to cisplatin in vitro and in cells...
  49. Cankorur Cetinkaya A, Eraslan S, Kirdar B. Transcriptomic response of yeast cells to ATX1 deletion under different copper levels. BMC Genomics. 2016;17:489 pubmed publisher
    ..for the first time, the possible role of ATX1 in cell cycle regulation, likewise its mammalian counterpart ATOX1, which was reported to play an important role in the copper-stimulated proliferation of non-small lung cancer ..
  50. Das A, Sudhahar V, Chen G, Kim H, Youn S, Finney L, et al. Endothelial Antioxidant-1: a Key Mediator of Copper-dependent Wound Healing in vivo. Sci Rep. 2016;6:33783 pubmed publisher
    ..Cu chaperone Antioxidant-1 (Atox1) in the cytosol supplies Cu to the secretory enzymes such as lysyl oxidase (LOX), while Atox1 in the nucleus ..
  51. Dolgova N, Yu C, Cvitkovic J, Hodak M, Nienaber K, Summers K, et al. Binding of Copper and Cisplatin to Atox1 Is Mediated by Glutathione through the Formation of Metal-Sulfur Clusters. Biochemistry. 2017;56:3129-3141 pubmed publisher
    ..used chemotherapy agent cisplatin is known to bind to copper-transporting proteins, including copper chaperone Atox1. Cisplatin interactions with Atox1 and other copper transporters are linked to cancer resistance to platinum-based ..
  52. Ogórek M, Lenartowicz M, Starzyński R, Jończy A, Staroń R, Doniec A, et al. Atp7a and Atp7b regulate copper homeostasis in developing male germ cells in mice. Metallomics. 2017;9:1288-1303 pubmed publisher
    ..the progression Atp7a expression profile corresponds to Slc31a1 (encoding copper importer CTR1) and Atox1 (encoding chaperon protein, which delivers copper from CTR1 to ATP7A and ATP7B) expression, suggesting that male ..
  53. Ansbacher T, Chourasia M, Shurki A. Copper-chaperones with dicoordinated Cu(I)--unique protection mechanism. Proteins. 2013;81:1411-9 pubmed publisher
  54. Hatori Y, Lutsenko S. The Role of Copper Chaperone Atox1 in Coupling Redox Homeostasis to Intracellular Copper Distribution. Antioxidants (Basel). 2016;5: pubmed publisher
    Human antioxidant protein 1 (Atox1) is a small cytosolic protein with an essential role in copper homeostasis. Atox1 functions as a copper carrier facilitating copper transfer to the secretory pathway...
  55. Manohar S, Leung N. Cisplatin nephrotoxicity: a review of the literature. J Nephrol. 2018;31:15-25 pubmed publisher
    ..Better understanding of these interactions could one day help devise better renoprotection that would not reduce its anti-tumor effects. ..
  56. Chen L, Luo J, Cui Z, Xue M, Wang L, Zhang X, et al. ATX3, ATX4, and ATX5 Encode Putative H3K4 Methyltransferases and Are Critical for Plant Development. Plant Physiol. 2017;174:1795-1806 pubmed publisher
    ..triple mutants resulted in exaggerated phenotypes when combined with the atx2 mutant but not with atx1 Together, we conclude that ATX3, ATX4, and ATX5 are redundantly required for H3K4 di- and ..
  57. Jiang C, Li Z, Zhang L, Tian Y, Dong D, Peng Y. Significance of hyphae formation in virulence of Candida tropicalis and transcriptomic analysis of hyphal cells. Microbiol Res. 2016;192:65-72 pubmed publisher
    ..hyphae up-regulated several genes involved in morphological differentiation and oxidative response, including IF2, Atx1, and Sod2. It appears that hyphal formation plays a vital role in the pathogenicity of C...
  58. Fernius J, Starkenberg A, Thor S. Bar-coding neurodegeneration: identifying subcellular effects of human neurodegenerative disease proteins using Drosophila leg neurons. Dis Model Mech. 2017;10:1027-1038 pubmed publisher
    ..affecting mitochondrial distribution, integrity or both, and A?1-42, tau, HTT128Q and ATX182Q affecting the F-actin network...
  59. Marzo T, Scaletti F, Michelucci E, Gabbiani C, Pescitelli G, Messori L, et al. Interactions of the organogold(III) compound Aubipyc with the copper chaperone Atox1: a joint mass spectrometry and circular dichroism investigation. Biometals. 2015;28:1079-85 pubmed publisher
    ..interactions occurring between the experimental anticancer organogold(III) drug, Aubipyc, and the copper chaperone Atox1, a key protein of the copper trafficking system...
  60. Matson Dzebo M, Ariöz C, Wittung Stafshede P. Extended functional repertoire for human copper chaperones. Biomol Concepts. 2016;7:29-39 pubmed publisher
    ..The human cytoplasmic Cu chaperone Atox1 delivers Cu to P1B-type ATPases in the Golgi network, for incorporation into Cu-dependent enzymes following the ..
  61. Shoshan M, Dekel N, Goch W, Shalev D, Danieli T, Lebendiker M, et al. Unbound position II in MXCXXC metallochaperone model peptides impacts metal binding mode and reactivity: Distinct similarities to whole proteins. J Inorg Biochem. 2016;159:29-36 pubmed publisher
    ..Studies with human antioxidant protein 1 (Atox1) and three of its mutants where S residues were replaced with Ala suggested that (a) the binding affinity is ..
  62. Shoshan M, Lehman Y, Goch W, Bal W, Tshuva E, Metanis N. Selenocysteine containing analogues of Atx1-based peptides protect cells from copper ion toxicity. Org Biomol Chem. 2016;14:6979-84 pubmed publisher
    ..than that of the reference peptide, and in the same order of magnitude as that measured for the native protein, Atox1. A stronger impact of residue 3 confirmed previous findings on its more dominant role in metal coordination...
  63. Zhang H, Wu X, Mehmood K, Chang Z, Li K, Jiang X, et al. Intestinal epithelial cell injury induced by copper containing nanoparticles in piglets. Environ Toxicol Pharmacol. 2017;56:151-156 pubmed publisher
    ..by differential expression levels of SOD, MDA and Metallothionein (MT) in addition to CTR1, SOD1, COX17, MT and ATOX1 genes expression...
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    ..Here we report small molecules that inhibit the human copper-trafficking proteins Atox1 and CCS, and so provide a selective approach to disrupt cellular copper transport...
  65. Xu F, Kuo T, Rosli Y, Liu M, Wu L, Chen L, et al. Trithorax Group Proteins Act Together with a Polycomb Group Protein to Maintain Chromatin Integrity for Epigenetic Silencing during Seed Germination in Arabidopsis. Mol Plant. 2018;11:659-677 pubmed publisher
    ..We investigated the interactions between the trxG genes, ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1) and ULTRAPETALA1 (ULT1), and the PcG gene EMBRYONIC FLOWER 1 (EMF1) during early development...
  66. Tao T, Liu F, Klomp L, Wijmenga C, Gitlin J. The copper toxicosis gene product Murr1 directly interacts with the Wilson disease protein. J Biol Chem. 2003;278:41593-6 pubmed
  67. Niemiec M, Dingeldein A, Wittung Stafshede P. Enthalpy-entropy compensation at play in human copper ion transfer. Sci Rep. 2015;5:10518 pubmed publisher
    ..After cell uptake, Cu is transferred, via direct protein-protein interactions, from the chaperone Atox1 to the Wilson disease protein (WD) for incorporation into Cu-dependent enzymes...
  68. Mercer S, La Fontaine S, Warr C, Burke R. Reduced glutathione biosynthesis in Drosophila melanogaster causes neuronal defects linked to copper deficiency. J Neurochem. 2016;137:360-70 pubmed publisher
    ..GSH could be required for binding Cu imported by Ctr1A and distributing it to chaperones, such as Mtn, CCS and Atox1. Alternatively, GSH could modify the copper-binding and transport activities of Atox1 and the ATP7 efflux protein ..
  69. Medici V, Weiss K. Genetic and environmental modifiers of Wilson disease. Handb Clin Neurol. 2017;142:35-41 pubmed publisher
    ..previously studied genes include copper metabolism domain-containing 1 (COMMD1), antioxidant 1 copper chaperone (ATOX1), X-linked inhibitor of apoptosis (XIAP), apolipoprotein E (APOE), hemochromatosis (HFE), and 5,10-..
  70. Sze C, Shi Z, Khairallah G, Feketeova L, O Hair R, Xiao Z, et al. Interaction of cisplatin and analogue Pt(en)Cl2 with the copper metallo-chaperone Atox1. Metallomics. 2013;5:946-54 pubmed publisher
    The human metallo-chaperone protein Atox1 features a high affinity Cu(I) binding site Cys(12)GlyGlyCys(15) (KD = 10(-17.4) M at pH 7.0) and delivers copper to the trans-Golgi network (TGN)...
  71. Banci L, Bertini I, Cantini F, Della Malva N, Migliardi M, Rosato A. The different intermolecular interactions of the soluble copper-binding domains of the menkes protein, ATP7A. J Biol Chem. 2007;282:23140-6 pubmed
    ..tail (MNK1-6) in solution by NMR spectroscopy and addressed its interaction with copper(I) and with copper(I)-HAH1, the physiological partner of ATP7A...
  72. Bost M, Piguet Lacroix G, Parant F, Wilson C. Molecular analysis of Wilson patients: direct sequencing and MLPA analysis in the ATP7B gene and Atox1 and COMMD1 gene analysis. J Trace Elem Med Biol. 2012;26:97-101 pubmed publisher
    ATP7B mutations result in Cu storage in the liver and brain in Wilson disease (WD). Atox1 and COMMD1 were found to interact with ATP7B and involved in copper transport in the hepatocyte...
  73. Itoh S, Kim H, Nakagawa O, Ozumi K, Lessner S, Aoki H, et al. Novel role of antioxidant-1 (Atox1) as a copper-dependent transcription factor involved in cell proliferation. J Biol Chem. 2008;283:9157-67 pubmed publisher
    ..Here we show that antioxidant-1 (Atox1), previously appreciated as a copper chaperone, represents a novel copper-dependent transcription factor that ..
  74. Niemiec M, Weise C, Wittung Stafshede P. In vitro thermodynamic dissection of human copper transfer from chaperone to target protein. PLoS ONE. 2012;7:e36102 pubmed publisher
    ..biophysical methods to deduce thermodynamic parameters of copper (Cu) transfer from the human copper chaperone Atox1 to the fourth metal-binding domain of the Wilson disease protein (WD4)...
  75. Hatori Y, Clasen S, Hasan N, Barry A, Lutsenko S. Functional partnership of the copper export machinery and glutathione balance in human cells. J Biol Chem. 2012;287:26678-87 pubmed publisher
    ..GSH/GSSG) pair controls the copper transport pathway by regulating the redox state of a copper chaperone Atox1. GSSG oxidizes copper-coordinating cysteines of Atox1 with the formation of an intramolecular disulfide...
  76. Dong D, Xu X, Yin W, Kang Y. Changes in copper concentrations affect the protein levels but not the mRNA levels of copper chaperones in human umbilical vein endothelial cells. Metallomics. 2014;6:554-9 pubmed publisher
    ..blot analyses showed that CTR1 silencing or TEPA treatment increased the protein levels of copper chaperone ATOX1 and copper chaperone for superoxide dismutase 1 (CCS-1), but decreased copper chaperone for cytochrome c oxidase (..
  77. Brose J, La Fontaine S, Wedd A, Xiao Z. Redox sulfur chemistry of the copper chaperone Atox1 is regulated by the enzyme glutaredoxin 1, the reduction potential of the glutathione couple GSSG/2GSH and the availability of Cu(I). Metallomics. 2014;6:793-808 pubmed publisher
    ..Such motifs are also present in copper-transporting proteins such as Atox1, a human cytosolic copper metallo-chaperone...
  78. Chen G, Sudhahar V, Youn S, Das A, Cho J, Kamiya T, et al. Copper Transport Protein Antioxidant-1 Promotes Inflammatory Neovascularization via Chaperone and Transcription Factor Function. Sci Rep. 2015;5:14780 pubmed publisher
    ..Here we uncover a novel role of Cu transport protein Antioxidant-1 (Atox1), which is originally appreciated as a Cu chaperone and recently discovered as a Cu-dependent transcription factor,..
  79. Kahra D, Kovermann M, Wittung Stafshede P. The C-Terminus of Human Copper Importer Ctr1 Acts as a Binding Site and Transfers Copper to Atox1. Biophys J. 2016;110:95-102 pubmed publisher
    ..to deduce Cu-binding residues, Cu affinity, and the ability to release Cu to the cytoplasmic Cu chaperone Atox1. Based on NMR assignments and bicinchoninic acid competition experiments, we demonstrate that Cu interacts in a 1:..
  80. Chiu Y, Justice A, Melton P. Longitudinal analytical approaches to genetic data. BMC Genet. 2016;17 Suppl 2:4 pubmed publisher
    ..method identified 5 SBP trajectories and association analyses identified a genome-wide significant locus, near ATOX1 (p = 1.0E(-8))...
  81. Belviso B, Galliani A, Lasorsa A, Mirabelli V, Caliandro R, Arnesano F, et al. Oxaliplatin Binding to Human Copper Chaperone Atox1 and Protein Dimerization. Inorg Chem. 2016;55:6563-73 pubmed publisher
    ..We investigated the reaction of the chaperone Atox1 with an activated form of oxaliplatin, the third platinum drug to reach worldwide approval...
  82. Andrés Bordería A, Andres F, Garcia Molina A, Perea García A, Domingo C, Puig S, et al. Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.). Plant Mol Biol. 2017;95:17-32 pubmed publisher
    ..Collectively, these results highlight the effects of rice copper status on iron homeostasis, which should be considered to obtain crops with optimized nutrient concentrations in edible parts. ..
  83. Sanokawa Akakura R, Dai H, Akakura S, Weinstein D, Fajardo J, Lang S, et al. A novel role for the immunophilin FKBP52 in copper transport. J Biol Chem. 2004;279:27845-8 pubmed
    ..We identified an interaction between FKBP52 domain I and Atox1, a copper-binding metallochaperone...
  84. Anastassopoulou I, Banci L, Bertini I, Cantini F, Katsari E, Rosato A. Solution structure of the apo and copper(I)-loaded human metallochaperone HAH1. Biochemistry. 2004;43:13046-53 pubmed
    The human metallochaperone HAH1 has been produced in Escherichia coli with four additional amino acids at the C-terminus and characterized in solution by NMR spectroscopy, both with and without copper(I)...
  85. Jeney V, Itoh S, Wendt M, Gradek Q, Ushio Fukai M, Harrison D, et al. Role of antioxidant-1 in extracellular superoxide dismutase function and expression. Circ Res. 2005;96:723-9 pubmed
    ..b>Atox1 is a copper chaperone proposed to deliver copper to the trans-Golgi network...
  86. Op t Holt B, Merz K. Insights into Cu(I) exchange in HAH1 using quantum mechanical and molecular simulations. Biochemistry. 2007;46:8816-26 pubmed
    The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK)...
  87. Hussain F, Wittung Stafshede P. Impact of cofactor on stability of bacterial (CopZ) and human (Atox1) copper chaperones. Biochim Biophys Acta. 2007;1774:1316-22 pubmed
    ..unfolding and thermodynamic stability of two copper chaperone proteins: Bacillus subtilis CopZ and Homo sapiens Atox1. We find that the unfolding reactions for apo- and Cu(I)-forms of CopZ and Atox1, induced by the chemical ..
  88. Simon I, Schaefer M, Reichert J, Stremmel W. Analysis of the human Atox 1 homologue in Wilson patients. World J Gastroenterol. 2008;14:2383-7 pubmed
    To analyze the metallochaperone antioxidant-1 (Atox1) gene sequence in Wilson disease patients...
  89. Vonk W, de Bie P, Wichers C, van den Berghe P, van der Plaats R, Berger R, et al. The copper-transporting capacity of ATP7A mutants associated with Menkes disease is ameliorated by COMMD1 as a result of improved protein expression. Cell Mol Life Sci. 2012;69:149-63 pubmed publisher
    ..Together, the presented data might provide a new direction for developing therapies to improve the residual exporting activity of unstable ATP7A mutant proteins, and suggests a potential role for COMMD1 in this process. ..
  90. Dolgova N, Nokhrin S, Yu C, George G, Dmitriev O. Copper chaperone Atox1 interacts with the metal-binding domain of Wilson's disease protein in cisplatin detoxification. Biochem J. 2013;454:147-56 pubmed publisher
    ..Platinum can also be transferred to MBD2 from copper chaperone Atox1, which was shown previously to bind cisplatin...
  91. Beaino W, Guo Y, Chang A, Anderson C. Roles of Atox1 and p53 in the trafficking of copper-64 to tumor cell nuclei: implications for cancer therapy. J Biol Inorg Chem. 2014;19:427-38 pubmed publisher
    ..We identified Atox1 as one of the proteins that binds copper in the nucleus...
  92. Kay K, Hamilton C, Le Brun N. Mass spectrometry of B. subtilis CopZ: Cu(i)-binding and interactions with bacillithiol. Metallomics. 2016;8:709-19 pubmed publisher
    CopZ from Bacillus subtilis is a well-studied member of the highly conserved family of Atx1-like copper chaperones...