ATG5

Summary

Gene Symbol: ATG5
Description: autophagy related 5
Alias: APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5, autophagy protein 5, APG5 autophagy 5-like, ATG5 autophagy related 5 homolog, apoptosis-specific protein
Species: human
Products:     ATG5

Top Publications

  1. Mizushima N, Yamamoto A, Hatano M, Kobayashi Y, Kabeya Y, Suzuki K, et al. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001;152:657-68 pubmed
    ..We recently found in yeast that a novel ubiquitin-like system, the Apg12-Apg5 conjugation system, is essential for autophagy...
  2. Tanida I, Tanida Miyake E, Komatsu M, Ueno T, Kominami E. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J Biol Chem. 2002;277:13739-44 pubmed
    ..Furthermore, hApg3p coimmunoprecipitates with hApg12p, and the overexpression of hApg3p facilitates the formation of the GFPhApg12p.thApg5p conjugate, suggesting that hApg3p cross-talks with the hApg12p conjugation system. ..
  3. Pyo J, Jang M, Kwon Y, Lee H, Jun J, Woo H, et al. Essential roles of Atg5 and FADD in autophagic cell death: dissection of autophagic cell death into vacuole formation and cell death. J Biol Chem. 2005;280:20722-9 pubmed
    ..However, its molecular event is unknown. Here, we show that Atg5, which is known to function in autophagy, contributes to autophagic cell death by interacting with Fas-associated ..
  4. Itoh T, Fujita N, Kanno E, Yamamoto A, Yoshimori T, Fukuda M. Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol Biol Cell. 2008;19:2916-25 pubmed publisher
    ..In addition, overexpression of the Rab33B binding domain of Atg16L suppressed autophagosome formation. Our findings suggest that Rab33 modulates autophagosome formation through interaction with Atg16L. ..
  5. Zhou X, Lu X, LV J, Yang H, Qin L, Zhao M, et al. Genetic association of PRDM1-ATG5 intergenic region and autophagy with systemic lupus erythematosus in a Chinese population. Ann Rheum Dis. 2011;70:1330-7 pubmed publisher
    Recent genome-wide association studies suggested the PRDM1-ATG5 gene region as a systemic lupus erythematosus (SLE)-associated locus both in Caucasian and Chinese populations; however, the candidate gene was still obscure and the possible ..
  6. Martin L, Gupta J, Jyothula S, Butsch Kovacic M, Biagini Myers J, Patterson T, et al. Functional variant in the autophagy-related 5 gene promotor is associated with childhood asthma. PLoS ONE. 2012;7:e33454 pubmed publisher
    ..Associations between two genes in the autophagy pathway, ATG5 and ATG7, with childhood asthma were investigated...
  7. Chen D, Zhu C, Wang X, Feng X, Pang S, Huang W, et al. A novel and functional variant within the ATG5 gene promoter in sporadic Parkinson's disease. Neurosci Lett. 2013;538:49-53 pubmed publisher
    ..We hypothesized that change expression levels of autophagy-related genes (ATG), including ATG5, may contribute to PD...
  8. Nishimura T, Kaizuka T, Cadwell K, Sahani M, Saitoh T, Akira S, et al. FIP200 regulates targeting of Atg16L1 to the isolation membrane. EMBO Rep. 2013;14:284-91 pubmed publisher
    ..Thus, FIP200 regulates not only early events but also late events of autophagosome formation through direct interaction with Atg16L1. ..
  9. Nemoto T, Tanida I, Tanida Miyake E, Minematsu Ikeguchi N, Yokota M, Ohsumi M, et al. The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification. J Biol Chem. 2003;278:39517-26 pubmed
    ..The inability of mutant Apg10pC161S to form any intermediate with MAP-LC3 has ruled out the possibility that MAP-LC3 interacts with Apg10p as a substrate. ..

More Information

Publications122 found, 100 shown here

  1. Yousefi S, Perozzo R, Schmid I, Ziemiecki A, Schaffner T, Scapozza L, et al. Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. Nat Cell Biol. 2006;8:1124-32 pubmed
    Autophagy-related gene (Atg) 5 is a gene product required for the formation of autophagosomes. Here, we report that Atg5, in addition to the promotion of autophagy, enhances susceptibility towards apoptotic stimuli...
  2. Jounai N, Takeshita F, Kobiyama K, Sawano A, Miyawaki A, Xin K, et al. The Atg5 Atg12 conjugate associates with innate antiviral immune responses. Proc Natl Acad Sci U S A. 2007;104:14050-5 pubmed
    ..We show here that the Atg5-Atg12 conjugate, a key regulator of the autophagic process, plays an important role in innate antiviral immune ..
  3. An C, Kim M, Yoo N, Park S, Lee S. Mutational and expressional analyses of ATG5, an autophagy-related gene, in gastrointestinal cancers. Pathol Res Pract. 2011;207:433-7 pubmed publisher
    There is mounting evidence that alterations of cell death processes are involved in cancer pathogenesis. ATG5 is a key regulator of autophagic and apoptotic cell death...
  4. Zhu K, Dunner K, McConkey D. Proteasome inhibitors activate autophagy as a cytoprotective response in human prostate cancer cells. Oncogene. 2010;29:451-62 pubmed publisher
    ..formation, stimulated autophagic flux, and upregulated expression of the autophagy-specific genes (ATGs) (ATG5 and ATG7) in some human prostate cancer cells and immortalized mouse embryonic fibroblasts (MEFs)...
  5. Guévin C, Manna D, Bélanger C, Konan K, Mak P, Labonte P. Autophagy protein ATG5 interacts transiently with the hepatitis C virus RNA polymerase (NS5B) early during infection. Virology. 2010;405:1-7 pubmed publisher
    Autophagy is an important cellular process by which ATG5 initiates the formation of double membrane vesicles (DMVs)...
  6. Gammoh N, Florey O, Overholtzer M, Jiang X. Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy. Nat Struct Mol Biol. 2013;20:144-9 pubmed publisher
    ..Here we have identified an interaction between mammalian FIP200 and ATG16L1, essential components of the ULK1 and ATG5 complexes, respectively...
  7. Hammond E, Brunet C, Johnson G, Parkhill J, Milner A, Brady G, et al. Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast. FEBS Lett. 1998;425:391-5 pubmed
    ..The ASP gene, known as hAPG5, can be transcribed to give mRNAs of 3.3 kbp, 2.5 kbp and 1...
  8. Mizushima N, Sugita H, Yoshimori T, Ohsumi Y. A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem. 1998;273:33889-92 pubmed
    ..This target protein was identified to be the human Apg5 homologue (hApg5)...
  9. Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, et al. Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J Cell Sci. 2003;116:1679-88 pubmed
    ..We have shown that, in yeast and mammalian cells, the Apg12-Apg5 protein conjugate, which is formed by a ubiquitin-like system, is essential for autophagosome formation...
  10. Song X, Zhu S, Xie Y, Liu J, Sun L, Zeng D, et al. JTC801 Induces pH-dependent Death Specifically in Cancer Cells and Slows Growth of Tumors in Mice. Gastroenterology. 2018;154:1480-1493 pubmed publisher
    ..or Mlkl-/- cells), ferroptosis (Gpx4-/- cells), or autophagy (Atg3-/-, Atg5-/-, Atg7-/-, or Sqstm1-/- cells)...
  11. Veskovic M, Mladenović D, Milenkovic M, Tosic J, Borozan S, Gopcevic K, et al. Betaine modulates oxidative stress, inflammation, apoptosis, autophagy, and Akt/mTOR signaling in methionine-choline deficiency-induced fatty liver disease. Eur J Pharmacol. 2019;848:39-48 pubmed publisher
    ..In addition, betaine increased the expression of autophagy activators beclin 1, autophagy-related (Atg)4 and Atg5, as well as the presence of autophagic vesicles and degradation of autophagic target sequestosome 1/p62 in the ..
  12. Reddy P, Yin X, Manczak M, Kumar S, Pradeepkiran J, Vijayan M, et al. Mutant APP and amyloid beta-induced defective autophagy, mitophagy, mitochondrial structural and functional changes and synaptic damage in hippocampal neurons from Alzheimer's disease. Hum Mol Genet. 2018;27:2502-2516 pubmed publisher
    ..and Fis1 and decreased levels fusion (Mfn1, Mfn2 and Opa1) biogenesis (PGC1α, NRF1, NRF2 & TFAM), autophagy (ATG5 & LC3BI, LC3BII), mitophagy (PINK1 & TERT, BCL2 & BNIPBL), synaptic (synaptophysin & PSD95) and ..
  13. Pajares M, Rojo A, Arias E, Díaz Carretero A, Cuervo A, Cuadrado A. Transcription factor NFE2L2/NRF2 modulates chaperone-mediated autophagy through the regulation of LAMP2A. Autophagy. 2018;14:1310-1322 pubmed publisher
    ..ACTB: actin, beta, ARE: antioxidant response element; ATG5: autophagy related 5; BACH1: BTB domain and CNC homolog 1; ChIP: chromatin immunoprecipitation; CMA: chaperone-..
  14. Ma M, Hui J, Zhang Q, Zhu Y, He Y, Liu X. Long non-coding RNA nuclear-enriched abundant transcript 1 inhibition blunts myocardial ischemia reperfusion injury via autophagic flux arrest and apoptosis in streptozotocin-induced diabetic rats. Atherosclerosis. 2018;277:113-122 pubmed publisher
    ..Cell viability of rat cardiomyocytes was analyzed by MTT assay. Levels of autophagy-related proteins Atg7, Atg5, LC3-II/LC3-I and p62 were determined by Western blot assay...
  15. Du X, Liu G, Loor J, Fang Z, Bucktrout R, Yang Y, et al. Impaired hepatic autophagic activity in dairy cows with severe fatty liver is associated with inflammation and reduced liver function. J Dairy Sci. 2018;101:11175-11185 pubmed publisher
    ..beclin 1 (BECN1), phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), autophagy-related gene (ATG) 3, ATG5, and ATG12, did not differ...
  16. Li S, Zhao H, Wang Y, Shao Y, Wang B, Wang Y, et al. Regulation of autophagy factors by oxidative stress and cardiac enzymes imbalance during arsenic or/and copper induced cardiotoxicity in Gallus gallus. Ecotoxicol Environ Saf. 2018;148:125-134 pubmed publisher
    ..associated gene 4B (Atg4B), microtubule associated light chains 3-II (LC3-II), autophagy associated gene 5 (Atg5) and Dynein...
  17. Frank B, Marcu A, de Oliveira Almeida Petersen A, Weber H, Stigloher C, Mottram J, et al. Autophagic digestion of Leishmania major by host macrophages is associated with differential expression of BNIP3, CTSE, and the miRNAs miR-101c, miR-129, and miR-210. Parasit Vectors. 2015;8:404 pubmed publisher
    ..The protein expression levels of autophagy related 5 (ATG5), BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), cathepsin E (CTSE), mechanistic target of ..
  18. Enkhbold C, Morine Y, Utsunomiya T, Imura S, Ikemoto T, Arakawa Y, et al. Dysfunction of liver regeneration in aged liver after partial hepatectomy. J Gastroenterol Hepatol. 2015;30:1217-24 pubmed publisher
    ..Immunohistochemical stainings for proliferating cell nuclear antigen, LC3, Atg5, and caspase-3 were used to quantify protein expression...
  19. Luo H, Sawashita J, Tian G, Liu Y, Li L, Ding X, et al. Extracellular deposition of mouse senile AApoAII amyloid fibrils induced different unfolded protein responses in the liver, kidney, and heart. Lab Invest. 2015;95:320-33 pubmed publisher
    ..factor 4 (Atf4), X-box-binding protein 1 splicing (Xbp1s), DNA-damage inducible transcript 3 (Ddit3), and autophagy protein 5 (Atg5). Moreover, apoptosis-positive cells were increased in the liver...
  20. Wang B, Cao P, Wang Z, Li Z, Wang Z, Ma J, et al. Interferon-γ-induced insufficient autophagy contributes to p62-dependent apoptosis of epithelial cells in chronic rhinosinusitis with nasal polyps. Allergy. 2017;72:1384-1397 pubmed publisher
    ..In some experiments, cultured NECs were transfected with small interfering RNA targeting p62 (sip62) or Atg5 (siAtg5). Cultured cells were analyzed with Western blotting and flow cytometry...
  21. Ma W, Sun X, Wang Y, Zhu Y, Han X, Liu N. Restoring mitochondrial biogenesis with metformin attenuates β-GP-induced phenotypic transformation of VSMCs into an osteogenic phenotype via inhibition of PDK4/oxidative stress-mediated apoptosis. Mol Cell Endocrinol. 2019;479:39-53 pubmed publisher
    ..In addition, inhibition of autophagy by small interfering RNA targeting Atg5 reduced mitochondrial biogenesis in VSMCs...
  22. Tang Y, Cai Q, Wang Y, Fan S, Zhang Z, Xiao M, et al. Protective effect of autophagy on endoplasmic reticulum stress induced apoptosis of alveolar epithelial cells in rat models of COPD. Biosci Rep. 2017;37: pubmed publisher
    ..The expression levels of light chain 3 (LC3-II), p62, Beclin-1, ATG5, ATG7, Caspase-12, and Caspase-3 were detected by Western blotting...
  23. Abate G, Ruminiski P, Kumar M, Singh K, Hamzabegovic F, Hoft D, et al. New Verapamil Analogs Inhibit Intracellular Mycobacteria without Affecting the Functions of Mycobacterium-Specific T Cells. Antimicrob Agents Chemother. 2015;60:1216-25 pubmed publisher
    ..antimycobacterial activities of verapamil and norverapamil were studied using bone marrow-derived macrophages from ATG5(flox/flox) (control) and ATG5(flox/flox) Lyz-Cre mice...
  24. Haim Y, Bluher M, Slutsky N, Goldstein N, Kloting N, Harman Boehm I, et al. Elevated autophagy gene expression in adipose tissue of obese humans: A potential non-cell-cycle-dependent function of E2F1. Autophagy. 2015;11:2074-2088 pubmed publisher
    ..N=69) and mRNA (N=437) were elevated in visceral fat of obese humans, correlating with increased expression of ATG5 (autophagy-related 5), MAP1LC3B/LC3B (microtubule-associated protein 1 light chain 3 ?), but not with proliferation/..
  25. Sun X, Li J, Zhao H, Wang Y, Liu J, Shao Y, et al. Synergistic effect of copper and arsenic upon oxidative stress, inflammation and autophagy alterations in brain tissues of Gallus gallus. J Inorg Biochem. 2018;178:54-62 pubmed publisher
    ..expressions of autophagy markers including phosphatidylinositol 3-kinase (PI3K), Akt, autophagy-related gene 5 (ATG5), microtubule-associated protein light chains 3 (LC3), ATG4B, and Becline1 in different regions of brains were up-..
  26. Nguyen L, Saad S, Tan Y, Pollock C, Chen H. Maternal high-fat diet induces metabolic stress response disorders in offspring hypothalamus. J Mol Endocrinol. 2017;59:81-92 pubmed publisher
    ..By contrast, protein levels of autophagy-related genes Atg5 and Atg7, as well as mitophagy marker Parkin, were slightly increased...
  27. Dupré Aucouturier S, Castells J, Freyssenet D, Desplanches D. Trichostatin A, a histone deacetylase inhibitor, modulates unloaded-induced skeletal muscle atrophy. J Appl Physiol (1985). 2015;119:342-51 pubmed publisher
    ..The autophagy-lysosome pathway (Bnip3 and microtubule-associated protein 1 light chain 3 proteins, Atg5, Gabarapl1, Ulk1, and cathepsin B and L mRNA) was not activated by unloading or TSA treatment...
  28. Zheng K, Li Y, Wang S, Wang X, Liao C, Hu X, et al. Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint. Autophagy. 2016;12:1593-613 pubmed publisher
    ..5-Fu induced prosurvival autophagy, and by inhibiting such autophagy, siRNAs against BECN1/beclin 1, ATG5, ATG7, and LC3B enhanced 5-Fu-induced autophagy-associated and apoptosis-independent cell death...
  29. Ansari M, Khan N, Ahmad I, Haqqi T. Parkin clearance of dysfunctional mitochondria regulates ROS levels and increases survival of human chondrocytes. Osteoarthritis Cartilage. 2018;26:1087-1097 pubmed publisher
    ..Autophagy was determined by Western blotting for ATG5, Beclin1, immunofluorescence staining and confocal microscopy. Gene expression was determined by RT-qPCR...
  30. Sirohi K, Kumari A, Radha V, Swarup G. A Glaucoma-Associated Variant of Optineurin, M98K, Activates Tbk1 to Enhance Autophagosome Formation and Retinal Cell Death Dependent on Ser177 Phosphorylation of Optineurin. PLoS ONE. 2015;10:e0138289 pubmed publisher
    ..Tbk1-induced cell death possibly involves autophagy, as shown by the effect of Atg5 knockdown, and requirement of autophagic function of OPTN...
  31. Han Y, Zhao Y, Li J, Li L, Li Y, Li S, et al. Stat3-Atg5 signal axis inducing autophagy to alleviate hepatic ischemia-reperfusion injury. J Cell Biochem. 2017;: pubmed publisher
    ..It was demonstrated by the effect of blocking autophagy using bafilomycin A1 or knocking Atg5 gene out reduces the anti-apoptotic effect of Stat3...
  32. Kang M, Kim M, Oh J, Kim Y, Song S, Kim S, et al. Frameshift mutations of autophagy-related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability. J Pathol. 2009;217:702-6 pubmed publisher
    ..In humans there are 16 known ATG genes, of which four (ATG2B, ATG5, ATG9B and ATG12) have mononucleotide repeats with seven or more nucleotides...
  33. Kunanopparat A, Kimkong I, Palaga T, Tangkijvanich P, Sirichindakul B, Hirankarn N. Increased ATG5-ATG12 in hepatitis B virus-associated hepatocellular carcinoma and their role in apoptosis. World J Gastroenterol. 2016;22:8361-8374 pubmed
    ..The expression of autophagy related genes ATG5, ATG12, ATG9A and ATG4B expression was analyzed in HepG2.2...
  34. Li X, Li Y, Fang S, Su J, Jiang J, Liang B, et al. Downregulation of autophagy-related gene ATG5 and GABARAP expression by IFN-λ1 contributes to its anti-HCV activity in human hepatoma cells. Antiviral Res. 2017;140:83-94 pubmed publisher
    ..Through PCR array, real time RT PCR, and western blot, two autophagy-related genes, ATG5 and GABARAP, were identified and verified to be down-regulated by IFN-λ1 treatment, either in HCV-uninfected ..
  35. Yang Z, Su Z, Dewitt J, Xie L, Chen Y, Li X, et al. Fluvastatin Prevents Lung Adenocarcinoma Bone Metastasis by Triggering Autophagy. EBioMedicine. 2017;19:49-59 pubmed publisher
    ..b>Atg5 or Atg7 deletion, or 3-methyadenine (3-MA) or Bafilomycin A1 (Baf A1) treatment prevented the fluvastatin-induced ..
  36. Arakawa S, Honda S, Torii S, Tsujioka M, Shimizu S. Monitoring of Atg5-Independent Mitophagy. Methods Mol Biol. 2018;1759:125-132 pubmed publisher
    ..There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the ..
  37. Miki Y, Shimoyama S, Kon T, Ueno T, Hayakari R, Tanji K, et al. Alteration of autophagy-related proteins in peripheral blood mononuclear cells of patients with Parkinson's disease. Neurobiol Aging. 2018;63:33-43 pubmed publisher
    ..of mRNAs for 6 core regulators of autophagy (UNC-51-like kinase [ULK] 3, autophagy-related [Atg] 2A, Atg4B, Atg5, Atg16L1, and histone deacetylase 6)...
  38. Min Z, Ting Y, Mingtao G, Xiaofei T, Dong Y, Chenguang Z, et al. Monitoring autophagic flux using p62/SQSTM1 based luciferase reporters in glioma cells. Exp Cell Res. 2018;363:84-94 pubmed publisher
    ..as an increased Luc2p-p62ΔU/Luc2p-p62 ratio, which could be neutralized by autophagy inhibitors or knockdown of ATG5. The performance of our autophagic flux detection system was comparable to a recently reported GFP-LC3-RFP-LC3ΔG ..
  39. Paul S, Kashyap A, Jia W, He Y, Schaefer B. Selective autophagy of the adaptor protein Bcl10 modulates T cell receptor activation of NF-?B. Immunity. 2012;36:947-58 pubmed publisher
    ..This homeostatic process may protect T cells from adverse consequences of unrestrained NF-?B activation, such as cellular senescence. ..
  40. Tiwari R, Parajuli P, Sylvester P. Synergistic anticancer effects of combined γ-tocotrienol and oridonin treatment is associated with the induction of autophagy. Mol Cell Biochem. 2015;408:123-37 pubmed publisher
    ..the expression of autophagy cellular markers including the conversion of LC3B-I to LC3B-II, beclin-1, Atg3, Atg7, Atg5-Atg12, LAMP-1 and cathepsin-D, and pretreatment with the autophagy inhibitors 3-methyladenine (3-MA) or bafilomycin ..
  41. Chang C, Chen P, Lu S, Hsieh M, Lin C, Lee H, et al. Propofol-enhanced autophagy increases motility and angiogenic capacity of cultured human umbilical vascular endothelial cells. Life Sci. 2015;142:49-59 pubmed publisher
    ..a stimulatory effect on autophagy flux by increasing expression of autophagy markers including mTOR, Beclin-1, Atg5, and LC3I/II, as well as a late endosomal indicator, Rab7...
  42. Rivera Vargas T, Cai Z, Shen Y, Dosset M, Benoit Lizon I, Martin T, et al. Selective degradation of PU.1 during autophagy represses the differentiation and antitumour activity of TH9 cells. Nat Commun. 2017;8:559 pubmed publisher
    ..CD4 T cells lacking Atg3 or Atg5 have increased interleukin-9 (IL-9) expression upon differentiation into TH9 cells relative to Atg3- or ..
  43. Chen X, Li L, Xu S, Bu W, Chen K, Li M, et al. Ultraviolet B radiation down-regulates ULK1 and ATG7 expression and impairs the autophagy response in human keratinocytes. J Photochem Photobiol B. 2018;178:152-164 pubmed publisher
    ..Here, we found that the mRNAs of some key ATG genes such as ULK1, ATG5 and ATG7 exhibited significantly lower levels in the skin tissues of the face and chest with solar ultraviolet ..
  44. Woodworth Hobbs M, Perry B, Rahnert J, Hudson M, Zheng B, Russ Price S. Docosahexaenoic acid counteracts palmitate-induced endoplasmic reticulum stress in C2C12 myotubes: Impact on muscle atrophy. Physiol Rep. 2017;5: pubmed publisher
    ..factor 4 (ATF4), spliced X-box binding protein 1 (XBP1s), C/EBP homologous protein (CHOP), and autophagy-related 5 (Atg5) as well as the protein levels of the PERK target nuclear factor erythroid 2-related factor (Nrf2), CHOP, and ..
  45. Huang W, Wen J, Lin R, Wei P, Huang F. Effects of mTOR/NF-?B signaling pathway and high thoracic epidural anesthesia on myocardial ischemia-reperfusion injury via autophagy in rats. J Cell Physiol. 2017;: pubmed publisher
    ..The mRNA and protein levels of mTOR, NF-?B, Fasl, Bcl-2 and Bax, and LC3-I, LC3-II, BNIP3 and Atg5 were detected by RT-qPCR and western blotting, respectively...
  46. Zhu L, Dong C, Sun C, Ma R, Yang D, Zhu H, et al. Rejuvenation of MPTP-induced human neural precursor cell senescence by activating autophagy. Biochem Biophys Res Commun. 2015;464:526-33 pubmed publisher
    ..expression, a decrease in SOD2 expression, and a decrease in expression of some key autophagy-related genes such as Atg5, Atg7, Atg12, and Beclin 1...
  47. Wu J, Yang C, Guo C, Li X, Yang N, Zhao L, et al. SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. Chem Biol Interact. 2016;244:94-104 pubmed publisher
    ..SZC015 induced autophagy in MCF-7 cells evidenced by the increase of LC3II/LC3I and up-regulation of Atg5 and beclin1...
  48. Song P, Li S, Wu H, Gao R, Rao G, Wang D, et al. Parkin promotes proteasomal degradation of p62: implication of selective vulnerability of neuronal cells in the pathogenesis of Parkinson's disease. Protein Cell. 2016;7:114-29 pubmed publisher
    ..interacts with and ubiquitinates p62 at the K13 to promote proteasomal degradation of p62 even in the absence of ATG5. Pathogenic mutations, knockdown of parkin or mutation of p62 at K13 prevented the degradation of p62...
  49. Pan C, Shah N, Kumar S, Wheeler S, Cinti J, Hoyt D, et al. Angiostatic actions of capsicodendrin through selective inhibition of VEGFR2-mediated AKT signaling and disregulated autophagy. Oncotarget. 2017;8:12675-12685 pubmed publisher
    ..strongly inducing autophagy as determined by increased expression of beclin1, autophagy-related gene (Atg) 3, Atg5 and LC3 cleavage...
  50. Li Y, Li X, Yang T, Wang J, Zhao X. The steroid hormone 20-hydroxyecdysone promotes switching from autophagy to apoptosis by increasing intracellular calcium levels. Insect Biochem Mol Biol. 2016;79:73-86 pubmed publisher
    ..High concentrations of 20E induced cleavage of ATG5 to NtATG5 and pro-caspase-3 to active caspase-3, which led to a switch from autophagy to apoptosis...
  51. Zheng X, Chen W, Hou H, Li J, Li H, Sun X, et al. Ginsenoside 20(S)-Rg3 induced autophagy to inhibit migration and invasion of ovarian cancer. Biomed Pharmacother. 2017;85:620-626 pubmed publisher
    ..in a dose-dependent manner as indicated by the upregulation of autophagy-associated molecules including LC3 II, ATG5 and ATG7...
  52. Han H, Zhou H, Sun W, Liu C, Hou X, Zhou W. [Knockdown of TRAIL-DR5 gene inhibits autophagy of ER-positive MCF-7 breast cancer cells induced by SAHA]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:1504-1510 pubmed
    ..The expressions of beclin-1, ATG3, ATG5, ATG7, ATG12, ATG16, ATG4A, ATG4B, ATG9B, LC3II, cathepsin B (CTSB) in the breast cancer cells were detected by ..
  53. Hu N, Kong L, Chen H, Li W, Qian A, Wang X, et al. Autophagy protein 5 enhances the function of rat EPCs and promotes EPCs homing and thrombus recanalization via activating AKT. Thromb Res. 2015;136:642-51 pubmed publisher
    ..Our objective was to investigate the functions of autophagy protein 5 (ATG5) in rat EPCs and its potential application in DVT...
  54. Cheng C, Lee Y, Chen P, Lin Y, Chou M, Kao Y. Free Fatty Acids Induce Autophagy and LOX-1 Upregulation in Cultured Aortic Vascular Smooth Muscle Cells. J Cell Biochem. 2017;118:1249-1261 pubmed publisher
    ..OA suppressed growth of VSMCs without apoptotic induction, but enhanced autophagy flux with elevation of Beclin-1, Atg5, and LC3I/II...
  55. Cao C, Han D, Su Y, Ge Y, Chen H, Xu A. Ginkgo biloba exocarp extracts induces autophagy in Lewis lung cancer cells involving AMPK / mTOR / p70S6k signaling pathway. Biomed Pharmacother. 2017;93:1128-1135 pubmed publisher
    ..reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the mRNA levels of Beclin1 and Atg5. Western Blot was used to detect the protein levels of Beclin1, Atg5, LC3I/II, p-AMPK, AMPK, p-mTOR, mTOR, p-p70S6k ..
  56. Wang Q, Guo Y, Jiang S, Dong M, Kuerban K, Li J, et al. A hybrid of coumarin and phenylsulfonylfuroxan induces caspase-dependent apoptosis and cytoprotective autophagy in lung adenocarcinoma cells. Phytomedicine. 2018;39:160-167 pubmed publisher
    ..Importantly, abolishing autophagy using inhibitors and ATG5 siRNA enhanced the cytotoxicity of compound 8b, indicating the cytoprotective role of autophagy in lung ..
  57. Zhang J, Qu X, Chu P, Xie D, Zhu L, Chao Y, et al. Low shear stress induces vascular eNOS uncoupling via autophagy-mediated eNOS phosphorylation. Biochim Biophys Acta Mol Cell Res. 2018;1865:709-720 pubmed publisher
    ..Interestingly, low SS-induced NO reduction could be reversed by rapamycin, WYE-354 or ATG5 overexpression vector via restoration of autophagic flux, but not by N-acetylcysteine or apocynin...
  58. Metzger S, Saukko M, Van Che H, Tong L, Puder Y, Riess O, et al. Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Hum Genet. 2010;128:453-9 pubmed publisher
    ..We initially tested eight single nucleotide polymorphisms in five Atg genes (Atg3, Atg5, Atg7, Atg16L1 and Beclin-1) for their frequency of ≥1%...
  59. Xi Y, Dhaliwal J, Ceizar M, Vaculik M, Kumar K, Lagace D. Knockout of Atg5 delays the maturation and reduces the survival of adult-generated neurons in the hippocampus. Cell Death Dis. 2016;7:e2127 pubmed publisher
    ..the role of autophagy in adult hippocampal neurogenesis, we genetically deleted Autophagy-related gene 5 (Atg5) that reduced autophagic flux and the survival of the progeny of dividing progenitor cells...
  60. Afsal K, Selvaraj P. Effect of 1,25-dihydroxyvitamin D3 on the expression of mannose receptor, DC-SIGN and autophagy genes in pulmonary tuberculosis. Tuberculosis (Edinb). 2016;99:1-10 pubmed publisher
    ..the expression of receptors such as mannose receptor (CD206) and DC-SIGN (CD209) as well as autophagy genes such as ATG5 and Beclin-1 (BECN1) in monocytes/macrophages of healthy controls (HCs) and pulmonary tuberculosis (PTB) patients ..
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    ..Depletion of LC3B, ATG5 or ATG7 reduced PB-induced cytotoxicity, indicating that autophagosome associated cell death participated in the ..
  62. Kemp V, Dautzenberg I, Limpens R, van den Wollenberg D, Hoeben R. Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery. Viruses. 2017;9: pubmed publisher
    ..Here, we demonstrate that reovirus induces autophagy in several cell lines, evident from the formation of Atg5-Atg12 complexes, microtubule-associated protein 1 light chain 3 (LC3) lipidation, p62 degradation, the appearance ..
  63. Kim S, Park S, Yu H, Ko K, Park H, Kim Y. The antipsychotic agent clozapine induces autophagy via the AMPK-ULK1-Beclin1 signaling pathway in the rat frontal cortex. Prog Neuropsychopharmacol Biol Psychiatry. 2018;81:96-104 pubmed publisher
    ..At the same time, the immunoreactivity of LC3-II and the Atg5-Atg12 conjugate, which indicate activation of autophagy, was increased...
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    ..Moreover, nine immune related genes (ABL1, ATF2, ATG5, C6, CD38, HMGB1, ICOSLG, IL12RB2 and PLAU) were significantly associated with patients' overall survival, among ..
  65. Teng A, Miyake T, Yokoe S, Zhang L, Rezende L, Sharma P, et al. Metformin increases degradation of phospholamban via autophagy in cardiomyocytes. Proc Natl Acad Sci U S A. 2015;112:7165-70 pubmed publisher
    ..experiments, together with lysosome (chloroquine and bafilomycin A1) and autophagic (3-methyladenine and Atg5 siRNA) antagonists...
  66. Xiong J, Wang K, He J, Zhang G, Zhang D, Chen F. TFE3 Alleviates Hepatic Steatosis through Autophagy-Induced Lipophagy and PGC1?-Mediated Fatty Acid ?-Oxidation. Int J Mol Sci. 2016;17:387 pubmed publisher
    ..on autophagy-induced lipophagy and PGC1?-mediated fatty acid ?-oxidation because blocking these pathways with an Atg5 small interfering RNA (siRNA) or PGC1? siRNA dramatically blunted the TFE3-mediated regulation of steatosis...
  67. Deželak M, Repnik K, Koder S, Ferkolj I, Potočnik U. A Prospective Pharmacogenomic Study of Crohn's Disease Patients during Routine Therapy with Anti-TNF-α Drug Adalimumab: Contribution of ATG5, NFKB1, and CRP Genes to Pharmacodynamic Variability. OMICS. 2016;20:296-309 pubmed publisher
    ..evaluated, during a routine therapy of Crohn's disease patients, the candidate autophagy-related genes ATG12 and ATG5 and the inflammation-related genes NFKB1, NFKBIA, and CRP as potential predictors of adalimumab treatment response (..
  68. Peng Q, Qin J, Zhang Y, Cheng X, Wang X, Lu W, et al. Autophagy maintains the stemness of ovarian cancer stem cells by FOXA2. J Exp Clin Cancer Res. 2017;36:171 pubmed publisher
    ..vitro were decreased when autophagy was inhibited by Bafilomycin A1(BafA1), Chloroquine(CQ) or autophagy related 5(ATG5) knockdown...
  69. Cheng X, Li S, Mao C, Wang M, Chen J, Wang F, et al. Serum Response Factor Promotes Dopaminergic Neuron Survival via Activation of Beclin 1-Dependent Autophagy. Neuroscience. 2018;371:288-295 pubmed publisher
    ..The protective effect of SRF was abolished when the expression of autophagy-related proteins Beclin 1 and Atg5 was suppressed...
  70. Yang S, Long L, Li D, Zhang J, Jin S, Wang F, et al. β-Guanidinopropionic acid extends the lifespan of Drosophila melanogaster via an AMP-activated protein kinase-dependent increase in autophagy. Aging Cell. 2015;14:1024-33 pubmed publisher
    ..On the other hand, when the expression of Atg5 protein, an essential protein for autophagy, was reduced by RNA interference (RNAi), the effect of β-GPA on ..
  71. Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, et al. Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay. elife. 2016;5: pubmed publisher
    ..We identified a homozygous missense mutation, changing a conserved amino acid, in ATG5 in two siblings with congenital ataxia, mental retardation, and developmental delay...
  72. Nazim U, Moon J, Lee Y, Seol J, Kim Y, Park S. Glipizide sensitizes lung cancer cells to TRAIL-induced apoptosis via Akt/mTOR/autophagy pathways. Oncotarget. 2017;8:100021-100033 pubmed publisher
    ..Inhibition of autophagy flux applying a specific inhibitor and genetically modified ATG5 siRNA enclosed glipizide-mediated enhancing effect of TRAIL...
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    ..Like p45ASP, myc-hApg5 cross-reacted with a c-Jun antibody and approximately 50% of myc-hApg5 was bound to a Triton X-100-insoluble ..
  74. Orozco G, Eyre S, Hinks A, Bowes J, Morgan A, Wilson A, et al. Study of the common genetic background for rheumatoid arthritis and systemic lupus erythematosus. Ann Rheum Dis. 2011;70:463-8 pubmed publisher
    ..These mapped to the TNFSF4, BANK1, TNIP1, PTTG1, UHRF1BP1, ATG5, JAZF1, BLK, KIAA1542, ITGAM and UBE2L3 loci...
  75. Kuang E, Okumura C, Sheffy Levin S, Varsano T, Shu V, Qi J, et al. Regulation of ATG4B stability by RNF5 limits basal levels of autophagy and influences susceptibility to bacterial infection. PLoS Genet. 2012;8:e1003007 pubmed publisher
    ..Collectively, the RNF5-mediated control of membranalATG4B reveals a novel layer in the regulation of LC3 processing and autophagy. ..
  76. Chen P, Luo X, Nie P, Wu B, Xu W, Shi X, et al. CQ synergistically sensitizes human colorectal cancer cells to SN-38/CPT-11 through lysosomal and mitochondrial apoptotic pathway via p53-ROS cross-talk. Free Radic Biol Med. 2017;104:280-297 pubmed publisher
    ..However, inhibition of autophagy using a small molecular inhibitor 3-methyladenine (3-MA) and ATG5 siRNA did not increase SN-38-induced cytotoxicity in CRC cells...
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    ..The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells ..
  78. Jung C, Seo M, Otto N, Kim D. ULK1 inhibits the kinase activity of mTORC1 and cell proliferation. Autophagy. 2011;7:1212-21 pubmed publisher
    ..In contrast, Atg5 deficiency did not have a significant effect on mTORC1 signaling and cell proliferation...
  79. Höcker R, Walker A, Schmitz I. Inhibition of autophagy through MAPK14-mediated phosphorylation of ATG5. Autophagy. 2013;9:426-8 pubmed publisher
    ..Moreover, we identified a novel post-translational modification of the crucial autophagy mediator ATG5, since MAPK14 directly phosphorylates ATG5 at threonine 75, which is evolutionarily conserved from yeast to human...
  80. Sood V, Sharma K, Gupta V, Saha D, Dhapola P, Sharma M, et al. ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons. Sci Rep. 2017;7:8789 pubmed publisher
    ..We have identified new targets of ATF3 and show that it binds to the promoter regions of Stat1, Irf9, Isg15 and Atg5 thereby inhibiting cellular antiviral signaling and autophagy...
  81. Ndoye A, Budina Kolomets A, Kugel C, Webster M, Kaur A, Behera R, et al. ATG5 Mediates a Positive Feedback Loop between Wnt Signaling and Autophagy in Melanoma. Cancer Res. 2017;77:5873-5885 pubmed publisher
    ..Genetic blockade of autophagy revealed an unexpected feedback loop whereby knocking down the autophagy factor ATG5 in Wnt5Ahigh cells decreased Wnt5A and increased β-catenin...
  82. Baumann P, Cech T. Pot1, the putative telomere end-binding protein in fission yeast and humans. Science. 2001;292:1171-5 pubmed
    ..It now appears that the protein that caps the ends of chromosomes is widely dispersed throughout the eukaryotic kingdom. ..
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    ..In 17alpha-AED treated T98G glioma cells there was an increase in the autophagy related proteins Atg5 and beclin-1...
  84. Höhn A, Sittig A, Jung T, Grimm S, Grune T. Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts. Free Radic Biol Med. 2012;53:1760-9 pubmed publisher
    ..However, such an inhibition is accompanied by an enhanced toxicity of the formed protein aggregates. Furthermore, it could be proven that macroautophagy is responsible for the uptake of lipofuscin into the lysosomes. ..
  85. Liu W, Guo J, Mu J, Tian L, Zhou D. Rapamycin Protects Sepsis-Induced Cognitive Impairment in Mouse Hippocampus by Enhancing Autophagy. Cell Mol Neurobiol. 2017;37:1195-1205 pubmed publisher
    ..05) and autophagy indicators (LC3-II, Atg5, Atg7) were increased, and P62 was decreased in rapamycin-treated CLP mice compared with the untreated (p < 0...
  86. Wang C, Zhang R, Zhou L, He J, Huang Q, Siyal F, et al. Intrauterine growth retardation promotes fetal intestinal autophagy in rats via the mechanistic target of rapamycin pathway. J Reprod Dev. 2017;63:547-554 pubmed publisher
    ..Analysis of intestinal autophagy showed that the mRNA expression of WIPI1, MAP1LC3B, Atg5, and Atg14 was also increased, while the protein levels of p62 were decreased in IUGR fetuses...
  87. Kim J, Hong S, Lee J, Han S, Roh K, Lee K, et al. Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners. Autophagy. 2015;11:75-87 pubmed publisher
    ..During this process, the ATG12-ATG5 conjugate requires 2 different binding partners, ATG16L1 for autophagosome elongation and TECPR1 for lysosomal ..
  88. Pandya V, Glubrecht D, Vos L, Hanson J, Damaraju S, Mackey J, et al. The pro-apoptotic paradox: the BH3-only protein Bcl-2 interacting killer (Bik) is prognostic for unfavorable outcomes in breast cancer. Oncotarget. 2016;7:33272-85 pubmed publisher
    ..We found a significant association between the autophagy marker ATG5 and Bik. Combined high expression level of ATG5 and Bik was a stronger predictor of outcome than either alone...
  89. Jiang Q, Hao R, Wang W, Gao H, Wang C. SIRT1/Atg5/autophagy are involved in the antiatherosclerosis effects of ursolic acid. Mol Cell Biochem. 2016;420:171-84 pubmed publisher
    ..Acetylation of lysine residue on Atg5 was assessed with immunoprecipitation...
  90. So K, Oh S. Heme oxygenase-1-mediated apoptosis under cadmium-induced oxidative stress is regulated by autophagy, which is sensitized by tumor suppressor p53. Biochem Biophys Res Commun. 2016;479:80-5 pubmed publisher
    ..The inhibition of autophagy using small interfering RNA (siRNA) for the autophagy-related gene atg5 enhanced HO-1, CHOP, and PARP-1 cleavage induced by Cd...
  91. Wang Y, Xia C, Lv Y, Li C, Mei Q, Li H, et al. Crosstalk Influence between P38MAPK and Autophagy on Mitochondria-Mediated Apoptosis Induced by Anti-Fas Antibody/Actinomycin D in Human Hepatoma Bel-7402 Cells. Molecules. 2017;22: pubmed publisher
    ..of apoptosis concomitant with autophagy, upregulation of p-P38MAPK and autophagy-associated gene proteins (Atg5-Atg12 protein complex, Atg7, Atg10, Beclin-1, LC3 I, and LC3 II), and downregulation of ΔΨm in Bel-7402 cells...
  92. Chen Z, Cao J, Zhou J, Liu H, Che L, Mizumura K, et al. Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2014;306:L1016-25 pubmed publisher
    ..In the present study we found that Cav-1 interacted with and regulated the expression of ATG12-ATG5, an ubiquitin-like conjugation system crucial for autophagosome formation, in lung epithelial Beas-2B cells...