ATG13

Summary

Gene Symbol: ATG13
Description: autophagy related 13
Alias: KIAA0652, PARATARG8, ATG13 autophagy related 13 homolog, autophagy-related protein 13
Species: human

Top Publications

  1. ncbi Prime-numbered Atg proteins act at the primary step in autophagy: unphosphorylatable Atg13 can induce autophagy without TOR inactivation
    Yoshiaki Kamada
    National Institute for Basic Biology, Okazaki, Japan
    Autophagy 6:415-6. 2010
  2. pmc The beginning of the end: how scaffolds nucleate autophagosome biogenesis
    Robin E Stanley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 24:73-81. 2014
  3. doi Distinct functions of Ulk1 and Ulk2 in the regulation of lipid metabolism in adipocytes
    Seung Hyun Ro
    Department of Biochemistry, Molecular Biology and Biophysics University of Minnesota Minneapolis, MN USA
    Autophagy 9:2103-14. 2013
  4. pmc Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae
    Shenshen Zou
    College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China
    Traffic 14:233-46. 2013
  5. pmc DNA damage signaling triggers the cytoplasm-to-vacuole pathway of autophagy to regulate cell cycle progression
    Vinay V Eapen
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA, USA
    Autophagy 9:440-1. 2013
  6. pmc CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable
    Daohong Chen
    Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, United States
    Biochem Biophys Res Commun 432:226-30. 2013
  7. pmc A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy
    Christine C Jao
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 110:5486-91. 2013
  8. doi Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae
    Kuninori Suzuki
    BioImaging Center, Graduate School of Frontier Sciences, University of Tokyo, FSB 101, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    J Cell Sci 126:2534-44. 2013
  9. doi Inhibition of autophagy via p53-mediated disruption of ULK1 in a SCA7 polyglutamine disease model
    Xin Yu
    Department of Neurochemistry, Stockholm University, 106 91, Stockholm, Sweden
    J Mol Neurosci 50:586-99. 2013
  10. pmc Anthricin Isolated from Anthriscus sylvestris (L.) Hoffm. Inhibits the Growth of Breast Cancer Cells by Inhibiting Akt/mTOR Signaling, and Its Apoptotic Effects Are Enhanced by Autophagy Inhibition
    Chang Hwa Jung
    Department of Food Biotechnology, University of Science and Technology, Daejon 305 806, Republic of Korea Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201 Anyangpangyo ro, Seongnam 463 746, Republic of Korea
    Evid Based Complement Alternat Med 2013:385219. 2013

Research Grants

  1. Regulation of TOR Signaling and Autophagy in Drosophila
    Thomas P Neufeld; Fiscal Year: 2010
  2. Regulation of TOR Signaling and Autophagy in Drosophila
    Thomas P Neufeld; Fiscal Year: 2010
  3. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
  4. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    Martha H Stipanuk; Fiscal Year: 2010
  5. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
  6. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007
  7. Nutritional Regulation of Cystein Dioxygenase
    MARTHA STIPANUK; Fiscal Year: 2007
  8. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2002

Scientific Experts

  • Yoshiaki Kamada
  • Ioannis P Nezis
  • Kuninori Suzuki
  • Xin Yu
  • Giichi Takaesu
  • Claudine Kraft
  • Sebastian Alers
  • Edmond Y Chan
  • MARTHA STIPANUK
  • Thomas P Neufeld
  • Do Hyung Kim
  • Chang Hwa Jung
  • Daniel J Klionsky
  • Robin E Stanley
  • Michael J Ragusa
  • Yuh Ying Yeh
  • James H Hurley
  • Christine C Jao
  • Nao Hosokawa
  • Vinay V Eapen
  • Heesun Cheong
  • Faqiang Li
  • Seung Hyun Ro
  • Fulvio Reggiori
  • Jun Lin Guan
  • Mondira Kundu
  • Noboru Mizushima
  • E Tian
  • Paul K Herman
  • Yukiko Kabeya
  • Farokh Dotiwala
  • Eleftherios Karanasios
  • XiaoChuang Wang
  • Olivier Roger Joassard
  • Vladimir V Kapitonov
  • James E Haber
  • Daohong Chen
  • Shenshen Zou
  • Tatsuhiko Ikeda
  • Tae Youl Ha
  • Midori Umekawa
  • Anthony M J Sanchez
  • Huijun Wei
  • Qianqian Liang
  • Endalkachew Ashenafi Alemu
  • Minchul Seo
  • Patricia Lisa-Santamaría
  • Richard D Vierstra
  • W Gao
  • Joung Hyuck Joo
  • Martin Graef
  • Anongpat Suttangkakul
  • Chun Shik Shin
  • Sandra Grass
  • Young Mi Kim
  • Takeshi Kaizuka
  • Fei Liu
  • Antje S Löffler
  • Oliver Florey
  • Elaine A Dunlop
  • Jinghua Han
  • Hong Zhang
  • Ricardo C Ferreira
  • Muriel Mari
  • Monika Kijanska
  • Itay Koren
  • Neil Michael Otto
  • Nobuo Munakata
  • Joseph S Stephan
  • Shun ichiro Iemura
  • Yu Yun Chang
  • Tohru Natsume
  • Ian G Ganley
  • Taichi Hara
  • Carol A Mercer
  • Tomoko Kawamata
  • Ludivine Sinzelle
  • Hessun Cheong
  • Wiebe H Meijer
  • Yoshinori Ohsumi
  • Jerzy Jurka
  • Jacob C Harrison
  • Yann Simon Gallot
  • Xiaoping Zhu
  • Xin Xu
  • Suijuan Qiu
  • Hitoshi Shimano
  • Yuria Saito
  • Simon A Walker
  • Min Li

Detail Information

Publications69

  1. ncbi Prime-numbered Atg proteins act at the primary step in autophagy: unphosphorylatable Atg13 can induce autophagy without TOR inactivation
    Yoshiaki Kamada
    National Institute for Basic Biology, Okazaki, Japan
    Autophagy 6:415-6. 2010
    ..The Atg1 kinase complex that comprises Atg1 and its binding prime-numbered Atg proteins (Atg11, Atg13, Atg17, Atg29 and Atg31) has long been a candidate for TORC1's downstream target...
  2. pmc The beginning of the end: how scaffolds nucleate autophagosome biogenesis
    Robin E Stanley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 24:73-81. 2014
    ..A multiprotein complex comprising the protein kinase autophagy-related protein 1 (Atg1) together with Atg13, Atg17, Atg29, and Atg31 (ULK1, ATG13, FIP200, and ATG101 in humans) has a pivotal role in the earliest steps of ..
  3. doi Distinct functions of Ulk1 and Ulk2 in the regulation of lipid metabolism in adipocytes
    Seung Hyun Ro
    Department of Biochemistry, Molecular Biology and Biophysics University of Minnesota Minneapolis, MN USA
    Autophagy 9:2103-14. 2013
    ..However, unlike other autophagy genes (Atg5, Atg13, Rb1cc1/Fip200, and Becn1) Ulk1 was dispensable for adipogenesis without affecting the expression of CCAAT/..
  4. pmc Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae
    Shenshen Zou
    College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China
    Traffic 14:233-46. 2013
    ..Genetic studies showed that the effect of the Trs130 mutation was downstream of Atg5 and upstream of Atg1, Atg13, Atg9 and Atg14 on the autophagic pathway...
  5. pmc DNA damage signaling triggers the cytoplasm-to-vacuole pathway of autophagy to regulate cell cycle progression
    Vinay V Eapen
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA, USA
    Autophagy 9:440-1. 2013
    ..GARP genes can be mimicked by hyperactivation of the Cvt pathway by overexpressing an unphosphorylatable form of ATG13 or by adding the TORC1 inhibitor rapamycin...
  6. pmc CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable
    Daohong Chen
    Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, United States
    Biochem Biophys Res Commun 432:226-30. 2013
    ..In addition, deletion or knockdown of the UKC component FIP200 or Atg13 only led to a partial reduction in LC3 lipidation, indicating that UKC could be also dispensable for this step ..
  7. pmc A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy
    Christine C Jao
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 110:5486-91. 2013
    Autophagy-related 13 (Atg13) is a key early-acting factor in autophagy and the major locus for nutrient-dependent regulation of autophagy by Tor. The 2...
  8. doi Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae
    Kuninori Suzuki
    BioImaging Center, Graduate School of Frontier Sciences, University of Tokyo, FSB 101, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    J Cell Sci 126:2534-44. 2013
    ..The PAS scaffold proteins (Atg13 and Atg17) and phosphatidylinositol 3-kinase complex I were localized to a position at the junction between the IM ..
  9. doi Inhibition of autophagy via p53-mediated disruption of ULK1 in a SCA7 polyglutamine disease model
    Xin Yu
    Department of Neurochemistry, Stockholm University, 106 91, Stockholm, Sweden
    J Mol Neurosci 50:586-99. 2013
    ..Together, this leads to a decreased capacity for autophagy induction via the ULK1-FIP200-Atg13-Atg101 complex...
  10. pmc Anthricin Isolated from Anthriscus sylvestris (L.) Hoffm. Inhibits the Growth of Breast Cancer Cells by Inhibiting Akt/mTOR Signaling, and Its Apoptotic Effects Are Enhanced by Autophagy Inhibition
    Chang Hwa Jung
    Department of Food Biotechnology, University of Science and Technology, Daejon 305 806, Republic of Korea Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201 Anyangpangyo ro, Seongnam 463 746, Republic of Korea
    Evid Based Complement Alternat Med 2013:385219. 2013
    ..Interestingly, blockage of autophagy by a pharmacological inhibitor or genetic deletion of ULK1 and Atg13 accelerated anthricin-induced apoptosis, suggesting that autophagy has cytoprotective effects...
  11. doi Regulation of Akt-mTOR, ubiquitin-proteasome and autophagy-lysosome pathways in response to formoterol administration in rat skeletal muscle
    Olivier Roger Joassard
    Laboratoire de Physiologie de l Exercice, Universite de Lyon, F 42023 Saint Etienne, France
    Int J Biochem Cell Biol 45:2444-55. 2013
    ..E1B interacting protein3 (Bnip3), and transcription factor EB (TFEB), as well as the protein content of Ulk1, Atg13, Atg5-Atg12 complex and p62/Sqstm1 remained unchanged or was even decreased in response to formoterol ..
  12. doi Dynamic association of the ULK1 complex with omegasomes during autophagy induction
    Eleftherios Karanasios
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB22 3AT, UK
    J Cell Sci 126:5224-38. 2013
    ..Here we show that the ULK1 complex colocalises with omegasomes in a PtdIns3P-dependent way. Live-cell imaging of Atg13 (a ULK1 complex component), omegasomes and LC3 establishes and annotates for the first time a complete sequence of ..
  13. pmc What the N-terminal domain of Atg13 looks like and what it does: a HORMA fold required for PtdIns 3-kinase recruitment
    Christine C Jao
    Laboratory of Molecular Biology National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda, MD USA
    Autophagy 9:1112-4. 2013
    b>Atg13 is a subunit of the Atg1 complex that is involved in autophagy. The middle and C-terminal regions of Atg13 are intrinsically disordered and rich in regulatory phosphorylation sites...
  14. doi Knockdown of mTOR by lentivirus‑mediated RNA interference suppresses atherosclerosis and stabilizes plaques via a decrease of macrophages by autophagy in apolipoprotein E‑deficient mice
    XiaoChuang Wang
    Department of Emergency Medicine, the Second Affiliated Hospital of Medical College, Xi an Jiaotong University, Xi an, Shaanxi 710004, P R China
    Int J Mol Med 32:1215-21. 2013
    ..In addition, western blot analysis revealed that the knockdown of mTOR increased autophagy‑related protein 13 (Atg13) dephosphorylation and light chain 3‑I/light chain 3‑II (LC3‑I/LC3‑II) ratios, both of which were ..
  15. pmc DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase
    Farokh Dotiwala
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02445, USA
    Proc Natl Acad Sci U S A 110:E41-9. 2013
    ..Autophagy in response to DNA damage can be induced in three different ways: by deleting the Golgi-associated retrograde protein complex (GARP), by adding rapamycin, or by overexpression of a dominant ATG13-8SA mutation.
  16. pmc Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis
    Michael J Ragusa
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 151:1501-12. 2012
    ..Upon induction of autophagy, Atg17-Atg31-Atg29 assembles with Atg1 and Atg13, which in turn initiates the formation of the phagophore...
  17. ncbi Inhibition of autophagy enhances sunitinib-induced cytotoxicity in rat pheochromocytoma PC12 cells
    Tatsuhiko Ikeda
    Department of Endocrine Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
    J Pharmacol Sci 121:67-73. 2013
    ..Indeed, direct mTORC1 inhibition initiates ULK1/2 autophosphorylation and subsequent Atg13 and FIP200 phosphorylation, inducing autophagy...
  18. pmc ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding
    Elaine A Dunlop
    Institute of Medical Genetics, Cardiff University, Cardiff, UK
    Autophagy 7:737-47. 2011
    ..This is a novel negative feedback loop that occurs upon activation of autophagy to maintain mTORC1 inhibition when nutrient supplies are limiting...
  19. doi TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy
    Giichi Takaesu
    Center for Integrated Medical Research, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo, 160 8582 Japan
    J Biochem 151:157-66. 2012
    ..Here we report that TAB2 and TAB3 bind to Beclin1 and colocalize in the cytoplasm. TAB2 also interacts with ATG13 and is phosphorylated by ULK1...
  20. ncbi Atg13 and FIP200 act independently of Ulk1 and Ulk2 in autophagy induction
    Sebastian Alers
    Department of Internal Medicine I University Clinic of Tübingen Tübingen, Germany
    Autophagy 7:1423-33. 2011
    ..of Unc-51-like kinases 1/2 (Ulk1/2), focal adhesion kinase family-interacting protein of 200 kDa (FIP200) and Atg13. Upon starvation, mTORC1-mediated repression of this complex is released, which then leads to Ulk1/2 activation...
  21. pmc Pro-tumorigenic function of autophagy in mammary oncogenesis
    Huijun Wei
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    Autophagy 8:129-31. 2012
    ..FIP200 (focal adhesion kinase family interacting protein of 200 kD) is a component of the ULK1-Atg13-FIP200-Atg101 complex that is essential for the induction of mammalian autophagy...
  22. pmc The incredible ULKs
    Sebastian Alers
    Department of Internal Medicine I, University Hospital of Tubingen, Otfried M and 252ller Str, 10, 72076 Tubingen, Germany
    Cell Commun Signal 10:7. 2012
    ..This review will mainly focus on the vertebrate Ulk1/2-Atg13-FIP200 protein complex, its function in autophagy initiation, its evolutionary descent from the yeast Atg1-Atg13-..
  23. pmc Ksp1 kinase regulates autophagy via the target of rapamycin complex 1 (TORC1) pathway
    Midori Umekawa
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    J Biol Chem 287:16300-10. 2012
    ..kinase functions in part as a negative regulator of autophagy; deletion of KSP1 facilitated dephosphorylation of Atg13, a TORC1 substrate, which correlates with enhanced autophagy...
  24. pmc Regulator and substrate: dual roles for the ATG1-ATG13 kinase complex during autophagic recycling in Arabidopsis
    Faqiang Li
    Department of Genetics, University of Wisconsin Madison, Madison, WI, USA
    Autophagy 8:982-4. 2012
    ..The process is tightly regulated by the autophagy-related 1(ATG1)-ATG13 kinase complex which is controlled by multiple nutrient-responsive upstream regulators that integrate nutrient ..
  25. pmc The protein factor-arrest 11 (Far11) is essential for the toxicity of human caspase-10 in yeast and participates in the regulation of autophagy and the DNA damage signaling
    Patricia Lisa-Santamaría
    Instituto de Biología Funcional y Genómica and Departamento de Microbiología y Genética, CSIC Universidad de Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain
    J Biol Chem 287:29636-47. 2012
    ..Furthermore, the protein Far11, which is a target of MAP kinases, is essential for the dephosphorylation of Atg13 and, consequently, for the induction of autophagy...
  26. pmc Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy
    Claudine Kraft
    Institute of Biochemistry, ETH Zurich, Switzerland
    EMBO J 31:3691-703. 2012
    ..The Atg1/ULK1 kinase is an essential component of the core autophagy machinery possibly activated by binding to Atg13 upon starvation...
  27. doi The C. elegans ATG101 homolog EPG-9 directly interacts with EPG-1/Atg13 and is essential for autophagy
    Qianqian Liang
    College of Life Sciences, Beijing Normal University, Beijing, China
    Autophagy 8:1426-33. 2012
    ..exhibit autophagy phenotypes characteristic of those associated with loss of function of unc-51/Atg1 and epg-1/Atg13. epg-9 encodes a protein with significant homology to mammalian ATG101. EPG-9 directly interacts with EPG-1/Atg13...
  28. pmc ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs
    Endalkachew Ashenafi Alemu
    Molecular Cancer Research Group, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 287:39275-90. 2012
    ..The mammalian ULK complex involved in autophagy consists of ULK1, ULK2, ATG13, FIP200, and ATG101...
  29. pmc An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy
    Yuh Ying Yeh
    Department of Molecular Genetics, Ohio State University, Columbus Ohio 43210, USA
    J Biol Chem 286:28931-9. 2011
    ..In this study we show that Atg13, an evolutionarily conserved regulator of Atg1, promotes the formation of a specific Atg1 self-interaction in the ..
  30. pmc ULK1 inhibits the kinase activity of mTORC1 and cell proliferation
    Chang Hwa Jung
    Department of Biochemistry, Molecular Biology and Biophysics University of Minnesota, Minneapolis, MN, USA
    Autophagy 7:1212-21. 2011
    ..Knockdown of Atg13, the binding partner of ULK1 and ULK2, mimicked the effects of ULK1 or ULK2 deficiency or knockdown...
  31. ncbi Dual role of Atg1 in regulation of autophagy-specific PAS assembly in Saccharomyces cerevisiae
    Hessun Cheong
    Life Sciences Institute, Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:724-6. 2008
    ..We found that Atg1, Atg13 and Atg17 play a similar role for PAS formation under autophagy-inducing conditions as seen for Atg11 during ..
  32. pmc Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy
    Joung Hyuck Joo
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 43:572-85. 2011
    ..and Hsp90-Cdc37 stabilizes and activates Ulk1, which in turn is required for the phosphorylation and release of Atg13 from Ulk1, and for the recruitment of Atg13 to damaged mitochondria...
  33. pmc The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis
    Anongpat Suttangkakul
    Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA
    Plant Cell 23:3761-79. 2011
    ..process is induced by various nutrient-responsive signaling cascades converging on the Autophagy-Related1 (ATG1)/ATG13 kinase complex...
  34. pmc Autophagy machinery mediates macroendocytic processing and entotic cell death by targeting single membranes
    Oliver Florey
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nat Cell Biol 13:1335-43. 2011
    ..These downstream components of the autophagy machinery, but not the upstream mammalian Tor (mTor)-regulated ULK-ATG13-FIP200 complex, facilitate lysosome fusion to single membranes and the degradation of internalized cargo...
  35. doi AMPK promotes skeletal muscle autophagy through activation of forkhead FoxO3a and interaction with Ulk1
    Anthony M J Sanchez
    INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F 34060 Montpellier, France
    J Cell Biochem 113:695-710. 2012
    ..Moreover, AMPK activation leads to the inhibition of mTORC1 and its subsequent dissociation of Ulk1, Atg13, and FIP200 complex...
  36. doi Paraproteins of familial MGUS/multiple myeloma target family-typical antigens: hyperphosphorylation of autoantigens is a consistent finding in familial and sporadic MGUS/MM
    Sandra Grass
    José Carreras Center for Immuno and Gene Therapy, Department Internal Medicine I, Saarland University Medical School, Homburg Saar, Germany
    Blood 118:635-7. 2011
    ..targeted P-7, and paraproteins from 4 affected members of a fourth family targeted P-8, which is encoded by the ATG13 gene...
  37. ncbi Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae
    Chun Shik Shin
    School of Biological Sciences and Research Center for Functional Cellulomics, Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
    Autophagy 7:854-62. 2011
    ..In addition, we found that Atg13 is gradually rephosphorylated during prolonged nitrogen starvation, and the kinase activity of Atg1 is required ..
  38. pmc Upregulation of human autophagy-initiation kinase ULK1 by tumor suppressor p53 contributes to DNA-damage-induced cell death
    W Gao
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell Death Differ 18:1598-607. 2011
    In yeast, activation of ATG1/ATG13 kinase complex initiates autophagy...
  39. ncbi Atg101, a novel mammalian autophagy protein interacting with Atg13
    Nao Hosokawa
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan
    Autophagy 5:973-9. 2009
    ..In yeast, Atg1 forms a complex with Atg11, Atg13, Atg17, Atg29 and Atg31, whereas mammalian Atg1 (ULK1/2) interacts with Atg13 and FIP200...
  40. ncbi epg-1 functions in autophagy-regulated processes and may encode a highly divergent Atg13 homolog in C. elegans
    E Tian
    College of Life Sciences, China Agricultural University, Beijing, China
    Autophagy 5:608-15. 2009
    ..epg-1 encodes a novel protein that shows limited sequence similarity to the yeast autophagy protein Atg13. epg-1 displays a similar expression pattern to, and directly interacts with, the C...
  41. ncbi A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy
    Carol A Mercer
    Department of Cancer and Cell Biology, Genome Research Institute, University of Cincinnati, Cincinnati, OH, USA
    Autophagy 5:649-62. 2009
    ..In yeast, the Atg1 kinase complex includes Atg1, Atg13, Atg17, and at least four other interacting proteins, some of which are phosphorylated in a TOR-dependent manner, ..
  42. pmc ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy
    Ian G Ganley
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Biol Chem 284:12297-305. 2009
    ..we report identification of a novel protein complex containing ULK1 and two additional protein factors, FIP200 and ATG13, all of which are essential for starvation-induced autophagy...
  43. pmc ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery
    Chang Hwa Jung
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 20:1992-2003. 2009
    ..Here, we identify that mTOR phosphorylates a mammalian homologue of Atg13 and the mammalian Atg1 homologues ULK1 and ULK2...
  44. pmc An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation
    Yu Yun Chang
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 20:2004-14. 2009
    ..Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila...
  45. pmc Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy
    Nao Hosokawa
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 20:1981-91. 2009
    ..Here, we report a novel mammalian autophagy factor, Atg13, which forms a stable approximately 3-MDa protein complex with ULK1 and FIP200...
  46. pmc Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism
    Edmond Y W Chan
    Secretory Pathways Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Mol Cell Biol 29:157-71. 2009
    ..other distinct regions that direct membrane association and interaction with the putative human homologue of Atg13, which we have here characterized. Atg13 is required for autophagy and Atg9 trafficking during autophagy...
  47. pmc Organization of the pre-autophagosomal structure responsible for autophagosome formation
    Tomoko Kawamata
    Department of Cell Biology, National Institute for Basic Biology, and School of Life Science, The Graduate University for Advanced Studies, Okazaki 444 8585, Japan
    Mol Biol Cell 19:2039-50. 2008
    ..The Atg1 kinase complex (Atg1-Atg13) is also essential for recruitment of Atg proteins to the PAS...
  48. pmc The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae
    Heesun Cheong
    Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:668-81. 2008
    ..The Atg1 C terminus mediates an interaction with Atg13 and Atg17, facilitating a structural role of Atg1 that is needed to efficiently organize an initial step of PAS ..
  49. ncbi ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes
    Wiebe H Meijer
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    Autophagy 3:106-16. 2007
    ..This suggests that in fungi an organism-specific form of selective autophagy may occur, for which specialized Atg proteins have evolved...
  50. pmc Atg17 regulates the magnitude of the autophagic response
    Heesun Cheong
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 16:3438-53. 2005
    ..As a result, it is defective in peroxisome degradation and is partially defective for autophagy. Atg17 interacts with both Atg1 and Atg13, via two coiled-coil domains, and these interactions facilitate its inclusion in the Atg1 complex.
  51. pmc Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy
    Yukiko Kabeya
    Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 16:2544-53. 2005
    ..Two-hybrid analyses and coimmunoprecipitation experiments demonstrated that Atg17 physically associates with Atg1-Atg13 complex, and this binding was enhanced under starvation conditions...
  52. doi mTORC1 phosphorylates the ULK1-mAtg13-FIP200 autophagy regulatory complex
    Edmond Y Chan
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
    Sci Signal 2:pe51. 2009
    ..mechanism in which mTORC1 phosphorylates the autophagy regulatory complex containing unc-51-like kinase 1 (ULK1), the mammalian Atg13 protein, and focal adhesion kinase interacting protein of 200 kD (FIP200) has also been elucidated.
  53. pmc The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy
    Joseph S Stephan
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 106:17049-54. 2009
    ..We show that in addition to Atg1, PKA directly phosphorylates Atg13, a conserved regulator of Atg1 kinase activity...
  54. doi Macroautophagy and its role in nutrient homeostasis
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Nutr Rev 67:677-89. 2009
    ..In addition, the recent placement of the Atg1*Atg13*Atg17 complex downstream of mTORC1 has provided at least one pathway by which changes in nutrient availability ..
  55. pmc Mitochondria regulate autophagy by conserved signalling pathways
    Martin Graef
    Department of Molecular and Cellular Biology, Davis University of California, Davis, CA, USA
    EMBO J 30:2101-14. 2011
    ..respiratory deficiency suppresses autophagic flux, autophagy gene induction, and recruitment of the Atg1-Atg13 kinase complex to the pre-autophagosomal structure by stimulating PKA activity...
  56. ncbi Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop
    Antje S Löffler
    Department of Internal Medicine I, University of Tubingen, Tubingen, Germany
    Autophagy 7:696-706. 2011
    Unc-51-like kinase 1 (Ulk1) plays a central role in autophagy induction. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)...
  57. ncbi FIP200, an essential component of mammalian autophagy is indispensible for fetal hematopoiesis
    Fei Liu
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    Autophagy 7:229-30. 2011
    ..family interacting protein of 200 kD) is a newly identified essential autophagy gene and a component of the ULK-Atg13-FIP200 complex...
  58. ncbi Activation of Atg1 kinase in autophagy by regulated phosphorylation
    Monika Kijanska
    Institute of Biochemistry, Department of Biology, ETH Züric, Zurich, Switzerland
    Autophagy 6:1168-78. 2010
    ..assembly site (PAS), or the proper assembly of the multisubunit Atg1 kinase complex and binding to its activator Atg13. However, mutation of either one of these sites results in a loss of Atg1 kinase activity and its function in ..
  59. ncbi Autophagy as a trigger for cell death: autophagic degradation of inhibitor of apoptosis dBruce controls DNA fragmentation during late oogenesis in Drosophila
    Ioannis P Nezis
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    Autophagy 6:1214-5. 2010
    ..Genetic inhibition: of autophagy-related genes atg1, atg13 and vps34 results in late-stage egg chambers containing persisting nurse cell nuclei without fragmented DNA and ..
  60. pmc An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
    Muriel Mari
    Department of Cell Biology and Institute of Biomembranes, University Medical Centre Utrecht, Utrecht University, 3584 CX Utrecht, The Netherlands
    J Cell Biol 190:1005-22. 2010
    ..In addition, genetic analyses indicate that Atg1, Atg13, and phosphatidylinositol-3-phosphate are involved in the further rearrangement of these initial membranes...
  61. pmc Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis
    Ioannis P Nezis
    Centre for Cancer Biomedicine, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    J Cell Biol 190:523-31. 2010
    ..Inhibition of autophagy by genetically removing the function of the autophagy genes atg1, atg13, and vps34 resulted in late stage egg chambers that contained persisting nurse cell nuclei without fragmented DNA ..
  62. doi Association of IFIH1 and other autoimmunity risk alleles with selective IgA deficiency
    Ricardo C Ferreira
    Genentech, Inc, South San Francisco, California, USA
    Nat Genet 42:777-80. 2010
    ..0 x 10(-4)) or random SNPs across the genome (P < 0.0001). These findings support the hypothesis that autoimmune mechanisms may contribute to the pathogenesis of IgAD...
  63. ncbi "Autophagy suite": Atg9 cycling in the cytoplasm to vacuole targeting pathway
    Nobuo Munakata
    Rikkyo University, Faculty of Science, Biophysics Laboratory, Tokyo, Japan
    Autophagy 6:679-85. 2010
    ..Additional proteins, including Atg11, Atg23 and Atg27 are involved in this anterograde movement, whereas Atg1-Atg13 and Atg2-Atg18 are required for the retrograde return to the peripheral sites...
  64. doi DAP1, a novel substrate of mTOR, negatively regulates autophagy
    Itay Koren
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Curr Biol 20:1093-8. 2010
    ..Thus far, only a few substrates of mTOR that control autophagy have been identified, including ULK1 and Atg13, both of which function as positive mediators...
  65. pmc Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae
    Yuh Ying Yeh
    Department of Molecular Genetics, The Ohio State University, Columbus Ohio 43210, USA
    Genetics 185:871-82. 2010
    ..required the presence of a functional Atg1 kinase domain and two known regulators of Atg1 activity, Atg13 and Atg17...
  66. pmc Tor directly controls the Atg1 kinase complex to regulate autophagy
    Yoshiaki Kamada
    Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Cell Biol 30:1049-58. 2010
    ..However, the mechanism by which TORC1 controls autophagy and the direct target of TORC1 activity remain unclear. Atg13 is an essential regulatory component of autophagy upstream of the Atg1 kinase complex, and here we show that yeast ..
  67. ncbi The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure
    Fulvio Reggiori
    Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Dev Cell 6:79-90. 2004
    ..We show that Atg9 and Atg23 cycle through the PAS in a process governed by the Atg1-Atg13 signaling complex...
  68. pmc Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes
    Ludivine Sinzelle
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 105:4715-20. 2008
    ..The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes...
  69. ncbi Harbinger transposons and an ancient HARBI1 gene derived from a transposase
    Vladimir V Kapitonov
    Genetic Information Research Institute, Mountain View, California 94040, USA
    DNA Cell Biol 23:311-24. 2004
    ..Based on this observation, we suggest that the hypothetical HARBI1 nucleases are also characterized by a strong DNA-target specificity...

Research Grants18

  1. Regulation of TOR Signaling and Autophagy in Drosophila
    Thomas P Neufeld; Fiscal Year: 2010
    ..the activity of a multicomponent complex containing the autophagy related kinase Atg1 and the phosphoprotein Atg13. Specific aims of this project are to: 1) test potential models of Atg1/13 complex regulation, including the ..
  2. Regulation of TOR Signaling and Autophagy in Drosophila
    Thomas P Neufeld; Fiscal Year: 2010
    ..the activity of a multicomponent complex containing the autophagy related kinase Atg1 and the phosphoprotein Atg13. Specific aims of this project are to: 1) test potential models of Atg1/13 complex regulation, including the ..
  3. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
    ....
  4. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    Martha H Stipanuk; Fiscal Year: 2010
    ....
  5. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2009
    ....
  6. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007
    ..Capacity for GSH synthesis, availability of the precursor amino acid cysteine, and steady-state GSH levels all contribute to the body's ability to defend itself against oxidative or chemical stressors. ..
  7. Nutritional Regulation of Cystein Dioxygenase
    MARTHA STIPANUK; Fiscal Year: 2007
    ..d.) To evaluate the physiological significance of CDO in the regulation of cellular cysteine (and gtutathione) level. ..
  8. NUTRITIONAL REGULATION OF CYSTEINE DIOXYGENASE
    MARTHA STIPANUK; Fiscal Year: 2002
    ..abstract_text> ..