ASH1L

Summary

Gene Symbol: ASH1L
Description: ash1 (absent, small, or homeotic)-like (Drosophila)
Alias: ASH1, ASH1L1, KMT2H, ASH1-like protein, absent small and homeotic disks protein 1 homolog, histone-lysine N-methyltransferase ASH1L, lysine N-methyltransferase 2H
Species: human

Top Publications

  1. pmc Alternative epigenetic chromatin states of polycomb target genes
    Yuri B Schwartz
    Department of Molecular Biology and Biochemistry, Rutgers University, Nelson Laboratories, Piscataway, New Jersey, United States of America
    PLoS Genet 6:e1000805. 2010
  2. pmc Feed-forward regulation of a cell fate determinant by an RNA-binding protein generates asymmetry in yeast
    Joshua J Wolf
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genetics 185:513-22. 2010
  3. pmc Mammalian ASH1L is a histone methyltransferase that occupies the transcribed region of active genes
    Gregory D Gregory
    Children s Hospital of Philadelphia, Division of Hematology, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 27:8466-79. 2007
  4. pmc Crystal structure of the human histone methyltransferase ASH1L catalytic domain and its implications for the regulatory mechanism
    Sojin An
    Structural Biology Laboratory of Epigenetics, Department of Biological Sciences, Graduate school of Nanoscience and Technology World Class University, KI for the BioCentury, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong Gu, Daejeon 305 701
    J Biol Chem 286:8369-74. 2011
  5. pmc Achaete-scute homologue-1 (ASH1) stimulates migration of lung cancer cells through Cdk5/p35 pathway
    Abeba Demelash
    Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Biol Cell 23:2856-66. 2012
  6. pmc huASH1 protein, a putative transcription factor encoded by a human homologue of the Drosophila ash1 gene, localizes to both nuclei and cell-cell tight junctions
    T Nakamura
    Kimmel Cancer Center, Thomas Jefferson Medical College, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 97:7284-9. 2000
  7. ncbi Expression pattern of a butterfly achaete-scute homolog reveals the homology of butterfly wing scales and insect sensory bristles
    R Galant
    Howard Hughes Medical Institute, Madison, Wisconsin, USA
    Curr Biol 8:807-13. 1998
  8. doi Regulation of H3K27me3 and H3K4me3 during early porcine embryonic development
    Yu Gao
    Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Copenhagen, Denmark
    Mol Reprod Dev 77:540-9. 2010
  9. pmc An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast
    R M Long
    Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226, USA
    J Cell Biol 153:307-18. 2001
  10. pmc A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes
    Boris Slobodin
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    RNA 16:2277-90. 2010

Scientific Experts

Detail Information

Publications130 found, 100 shown here

  1. pmc Alternative epigenetic chromatin states of polycomb target genes
    Yuri B Schwartz
    Department of Molecular Biology and Biochemistry, Rutgers University, Nelson Laboratories, Piscataway, New Jersey, United States of America
    PLoS Genet 6:e1000805. 2010
    ..that critical for the active state of PcG target genes are the histone methyltransferases Trithorax (TRX) and ASH1. Here we analyze the repertoire of alternative states in which PcG target genes are found in different Drosophila ..
  2. pmc Feed-forward regulation of a cell fate determinant by an RNA-binding protein generates asymmetry in yeast
    Joshua J Wolf
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genetics 185:513-22. 2010
    ..Khd1 represses transcription of FLO11 indirectly through its regulation of ASH1 mRNA. Khd1 also represses FLO11 through a post-transcriptional mechanism independent of ASH1...
  3. pmc Mammalian ASH1L is a histone methyltransferase that occupies the transcribed region of active genes
    Gregory D Gregory
    Children s Hospital of Philadelphia, Division of Hematology, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 27:8466-79. 2007
    ..To identify the enzymes responsible for placing these modifications, we examined ASH1L, the mammalian homolog of the Drosophila melanogaster Trithorax group (TrxG) protein Ash1...
  4. pmc Crystal structure of the human histone methyltransferase ASH1L catalytic domain and its implications for the regulatory mechanism
    Sojin An
    Structural Biology Laboratory of Epigenetics, Department of Biological Sciences, Graduate school of Nanoscience and Technology World Class University, KI for the BioCentury, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong Gu, Daejeon 305 701
    J Biol Chem 286:8369-74. 2011
    ..Here, we present the crystal structure of the human ASH1L catalytic domain, showing its substrate binding pocket blocked by a loop from the post-SET domain...
  5. pmc Achaete-scute homologue-1 (ASH1) stimulates migration of lung cancer cells through Cdk5/p35 pathway
    Abeba Demelash
    Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Biol Cell 23:2856-66. 2012
    ..We were also able to show that p35 was a direct target for hASH1. In conclusion, induction of Cdk5 activity is a novel mechanism through which hASH1 may regulate migration in lung carcinogenesis...
  6. pmc huASH1 protein, a putative transcription factor encoded by a human homologue of the Drosophila ash1 gene, localizes to both nuclei and cell-cell tight junctions
    T Nakamura
    Kimmel Cancer Center, Thomas Jefferson Medical College, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 97:7284-9. 2000
    ..trithorax (trx)and ash1 (absent, small, or homeotic 1) are members of the Drosophila trx group...
  7. ncbi Expression pattern of a butterfly achaete-scute homolog reveals the homology of butterfly wing scales and insect sensory bristles
    R Galant
    Howard Hughes Medical Institute, Madison, Wisconsin, USA
    Curr Biol 8:807-13. 1998
    ....
  8. doi Regulation of H3K27me3 and H3K4me3 during early porcine embryonic development
    Yu Gao
    Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Copenhagen, Denmark
    Mol Reprod Dev 77:540-9. 2010
    ..EZH2), EZH2 co-factors (EED and SUZ12) and demethylases (JMJD3 and UTX), as well as H3K4me3's methylases (ASH1L and MLL1) and demethylase (RBP2) in porcine pre-implantation embryos...
  9. pmc An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast
    R M Long
    Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226, USA
    J Cell Biol 153:307-18. 2001
    The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae...
  10. pmc A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes
    Boris Slobodin
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    RNA 16:2277-90. 2010
    ..g., ASH1-She2; β-Actin-IMP1). By isolating tagged OXA1 mRNA using RaPID, we could identify a yeast COPI subunit (i.e...
  11. ncbi The Arabidopsis SET-domain protein ASHR3 is involved in stamen development and interacts with the bHLH transcription factor ABORTED MICROSPORES (AMS)
    Tage Thorstensen
    Department of Molecular Biosciences, University of Oslo, PO Box 1041, Blindern, 0316 Oslo, Norway
    Plant Mol Biol 66:47-59. 2008
    ..analyzed the Arabidopsis trxG protein ASHR3 which has a SET domain and pre- and post-SET domains similar to that of Ash1 in Drosophila...
  12. pmc She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae
    Sharon M Landers
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Eukaryot Cell 8:1072-83. 2009
    ..One mechanism for sorting proteins is through RNA localization. In Saccharomyces cerevisiae, ASH1 mRNA localizes to the distal tip of the bud, resulting in the asymmetric sorting of the transcriptional repressor ..
  13. ncbi Trithorax-group protein ASH1 methylates histone H3 lysine 36
    Yujiro Tanaka
    Genome Structure and Expression, School of Biomedical Science, and Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyoku, Tokyo, Japan
    Gene 397:161-8. 2007
    Drosophila discs absent, small, or homeotic-1 (ASH1) is a member of trithorax-group proteins that play essential roles in epigenetic regulation of Hox genes...
  14. ncbi Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p
    Nicolas Paquin
    Departement de Biochimie, Universite de Montreal, Montreal, QC H3C 3J7, Canada
    Mol Cell 26:795-809. 2007
    In S. cerevisiae, the ASH1 mRNA is localized at the bud tip of late-anaphase cells, resulting in the exclusive sorting of Ash1p to the daughter cell nucleus...
  15. pmc ASH1 mRNA localization in three acts
    D L Beach
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Mol Biol Cell 12:2567-77. 2001
    ..In budding yeast, ASH1 mRNA is translocated via actin and myosin to the tip of growing cells...
  16. ncbi The protein kinase Pho85 is required for asymmetric accumulation of the Ash1 protein in Saccharomyces cerevisiae
    H J McBride
    Department of Pathology, University of Utah, Salt Lake City, UT 84132, USA
    Mol Microbiol 42:345-53. 2001
    The Ash1 protein is a daughter cell-specific repressor of HO gene transcription in Saccharomyces cerevisiae...
  17. pmc The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast
    Kenji Irie
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    EMBO J 21:1158-67. 2002
    RNA localization is a widespread mechanism for achieving localized protein synthesis. In Saccharomyces cerevisiae, Ash1 is a specific repressor of transcription that localizes asymmetrically to the daughter cell nucleus through the ..
  18. ncbi [RNA localization in the cytoplasm]
    Eugenia Basyuk
    IGMM CNRS UMR 5535, Universite Montpellier II, IFR 122, 1919, route de Mende, 34293 Montpellier Cedex 5, France
    Med Sci (Paris) 20:669-73. 2004
    ..In this review, the various functions of RNA localization will be presented, and by analyzing two examples, Ash1 mRNA in yeast and retroviral genomic RNAs in mammals, the reader will be taken step by step into the detailed ..
  19. pmc Histone H2A ubiquitination inhibits the enzymatic activity of H3 lysine 36 methyltransferases
    Gang Yuan
    From the College of Life Sciences, Beijing Normal University, Beijing, 100875
    J Biol Chem 288:30832-42. 2013
    ..Notably, all of the H3K36-specific methyltransferases, including ASH1L, HYPB, NSD1, and NSD2 were inhibited by ubH2A, whereas the other histone methyltransferases, including PRC2, G9a, ..
  20. ncbi Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6/E7 transgenic mice
    Laura Carraresi
    Department of Physiopathology and Experimental Medicine, University of Siena, Via Aldo Moro, 53100 Siena, Italy
    Cancer Lett 231:65-73. 2006
    ..Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash1, CgrA, Scg2...
  21. ncbi The cAMP pathway in combination with BMP2 regulates Phox2a transcription via cAMP response element binding sites
    Chutamas Benjanirut
    Department of Basic Medical Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 281:2969-81. 2006
    ..Because the E-box binds bHLH proteins like ASH1 induced in NC cells by BMP2, we propose this novel 170-bp cis-acting element is a composite site, mediating the ..
  22. ncbi RNA asymmetric distribution and daughter/mother differentiation in yeast
    Xavier Darzacq
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Curr Opin Microbiol 6:614-20. 2003
    Active transport and localized translation of the ASH1 mRNA at the bud tip of the budding yeast Saccharomyces cerevisiae is an essential process that is required for the regulation of the mating type switching...
  23. pmc Ash1 protein, an asymmetrically localized transcriptional regulator, controls filamentous growth and virulence of Candida albicans
    Diane O Inglis
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143, USA
    Mol Cell Biol 22:8669-80. 2002
    ..Here we describe the characterization of C. albicans ASH1, a gene that encodes an asymmetrically localized transcriptional regulatory protein involved in this response...
  24. pmc Ash1l methylates Lys36 of histone H3 independently of transcriptional elongation to counteract polycomb silencing
    Hitomi Miyazaki
    Division of Molecular Genetics and Epigenetics, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, 5 1 1 Nabeshima, Saga City, Saga, Japan Precursory Research for Embryonic Science and Technology PRESTO, Japan Science and Technology Agency JST, 4 1 8 Honcho, Kawaguchi City, Saitama, Japan
    PLoS Genet 9:e1003897. 2013
    ..Here, using genetics and DRB, we show that mammalian Absent, small, or homeotic discs 1-like (Ash1l), a member of the trithorax group proteins, methylates Lys36 of histone H3 to promote the establishment of Hox ..
  25. pmc Identification and Characterization of FGF2-Dependent mRNA: microRNA Networks During Lens Fiber Cell Differentiation
    Louise Wolf
    Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461
    G3 (Bethesda) 3:2239-55. 2013
    ..and Nfib (DNA-binding transcription factors); Cnot6, Cpsf6, Dicer1, and Tnrc6b (RNA to miRNA processing); and Ash1l, Med1/PBP, and Kdm5b/Jarid1b/Plu1 (chromatin remodeling)...
  26. pmc The trithorax group proteins Kismet and ASH1 promote H3K36 dimethylation to counteract Polycomb group repression in Drosophila
    Kristel M Dorighi
    Department of Molecular Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Development 140:4182-92. 2013
    ..KIS promotes transcription elongation, facilitates the binding of the trithorax group histone methyltransferases ASH1 and TRX to active genes, and counteracts repressive methylation of histone H3 on lysine 27 (H3K27) by Polycomb ..
  27. pmc Previous history of chronic stress changes the transcriptional response to glucocorticoid challenge in the dentate gyrus region of the male rat hippocampus
    Nicole A Datson
    Division of Medical Pharmacology, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
    Endocrinology 154:3261-72. 2013
    ..affected the expression of several genes involved in chromatin structure and epigenetic processes, including Asf1, Ash1l, Hist1h3f, and Tp63...
  28. pmc PfSETvs methylation of histone H3K36 represses virulence genes in Plasmodium falciparum
    Lubin Jiang
    Key Laboratory of Molecular Virology and Immunology, Unit of Human Parasite Molecular and Cell Biology, Institut Pasteur of Shanghai, Shanghai 200031, China
    Nature 499:223-7. 2013
    ..falciparum variant-silencing SET gene (here termed PfSETvs), which encodes an orthologue of Drosophila melanogaster ASH1 and controls histone H3 lysine 36 trimethylation (H3K36me3) on var genes, results in the transcription of virtually ..
  29. pmc The histone methyltransferases Trithorax and Ash1 prevent transcriptional silencing by Polycomb group proteins
    Tetyana Klymenko
    EMBL, Gene Expression Programme, Meyerhofstrasse 1, Heidelberg 69117, Germany
    EMBO Rep 5:373-7. 2004
    ..The trxG proteins Ash1 and hTRX and the PcG repressor E(z) are histone methyltransferases (HMTases) that methylate distinct lysine ..
  30. pmc Assembly of mRNA-protein complexes for directional mRNA transport in eukaryotes--an overview
    Ralf Peter Jansen
    Interfaculty Institute for Biochemistry, University of Tubingen, Tubingen, Germany
    Curr Protein Pept Sci 13:284-93. 2012
    ..The first example is She2p/She3p-dependent localization of ASH1 mRNA in budding yeast...
  31. pmc A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophy
    Daphne S Cabianca
    Dulbecco Telethon Institute at San Raffaele Scientific Institute, Division of Regenerative Medicine, Stem Cells, and Gene Therapy, Milan, Italy
    Cell 149:819-31. 2012
    ..DBE-T recruits the Trithorax group protein Ash1L to the FSHD locus, driving histone H3 lysine 36 dimethylation, chromatin remodeling, and 4q35 gene transcription...
  32. pmc Histone methyltransferase ASH1 orchestrates fibrogenic gene transcription during myofibroblast transdifferentiation
    Maria Jesus Perugorria
    Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK
    Hepatology 56:1129-39. 2012
    ..quiescent and activated primary HSCs we found a number of histone methyltransferases including MLL1, MLL5, Set1 and ASH1 to be highly up-regulated during transdifferentiation of HSCs...
  33. pmc Classifying transcription factor targets and discovering relevant biological features
    Dustin T Holloway
    Molecular Biology Cell Biology and Biochemistry Department, Boston University, 5 Cummington Street, Boston, MA, USA
    Biol Direct 3:22. 2008
    ..The classifiers combine 8 genomic datasets covering a broad range of measurements including sequence conservation, sequence overrepresentation, gene expression, and DNA structural properties...
  34. ncbi mRNA trafficking in fungi
    Kathi Zarnack
    Department for Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043, Marburg, Germany
    Mol Genet Genomics 278:347-59. 2007
    ..The best-understood example is transport of ASH1 mRNA to the distal pole of the incipient daughter cell...
  35. pmc Functional dissection of the ash2 and ash1 transcriptomes provides insights into the transcriptional basis of wing phenotypes and reveals conserved protein interactions
    Sergi Beltran
    Departament de Genetica, Universitat de Barcelona, Diagonal 645, Barcelona, Spain
    Genome Biol 8:R67. 2007
    The trithorax group (trxG) genes absent, small or homeotic discs 1 (ash1) and 2 (ash2) were isolated in a screen for mutants with abnormal imaginal discs...
  36. pmc Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation
    Yingfeng Deng
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 22:1037-50. 2008
    ..In the yeast Saccharomyces cerevisae, silencing of ASH1 mRNA before it is localized to the bud cortex in late anaphase is critical for asymmetric segregation of Ash1p to ..
  37. doi Roles of achaete-scute homologue 1 in DKK1 and E-cadherin repression and neuroendocrine differentiation in lung cancer
    Hirotaka Osada
    Division of Molecular Oncology, Aichi Cancer Center Research Institute, Nagoya 464 8681, Japan
    Cancer Res 68:1647-55. 2008
    The proneural basic-helix-loop-helix protein achaete-scute homologue 1 (ASH1) is expressed in a very limited spectrum of normal and cancerous cells in a lineage-specific manner, including normal pulmonary neuroendocrine cells and lung ..
  38. doi Gene expression profiling in hepatocellular carcinoma: upregulation of genes in amplified chromosome regions
    Britta Skawran
    Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany
    Mod Pathol 21:505-16. 2008
    ..down the number of dysregulated genes to 18, with 7 genes localised on 1q22 (SCAMP3, IQGAP3, PYGO2, GPATC4, ASH1L, APOA1BP, and CCT3). In hepatocellular adenoma 26 genes in bands 11p15, 11q12, and 12p13 were upregulated...
  39. pmc mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae
    Stella Aronov
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:3441-55. 2007
    ..Asymmetric mRNA localization depends upon factors that facilitate ASH1 mRNA localization (e.g...
  40. doi Local regulation of mRNA translation: new insights from the bud
    Nicolas Paquin
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec, H3C 3J7 Canada
    Trends Cell Biol 18:105-11. 2008
    ..To avoid any ectopic expression, translation of the bud-localized ASH1 mRNA is repressed by the translational repressors Puf6p and Khd1p during its transport...
  41. pmc Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA
    Brian D Dunn
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 178:1193-206. 2007
    ..that the unique motility of Myo4p is not due to its motor domain and that the motor domain of Myo2p can transport ASH1 mRNA in vivo...
  42. ncbi The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells
    Julian Vasilescu
    Ottawa Institute of Systems Biology, 451 Smyth Road, Ottawa, Ontario, K1H 8M5, Canada
    J Proteome Res 6:298-305. 2007
    ..The Proteomic Reactor is a novel technology that facilitates the analysis of affinity-purified proteins and has the potential to aid future biological studies...
  43. ncbi Directional mRNA transport in eukaryotes: lessons from yeast
    M Muller
    Gene Center of the Ludwig Maximilians, University Munich and GSF, Institute of Molecular Immunology, Feodor Lynen Strasse 25, 81377, Munich, Germany
    Cell Mol Life Sci 64:171-80. 2007
    ..provides a more detailed description of the most comprehensively studied mRNA translocation complex to date: the ASH1 messenger ribonucleoprotein particle (mRNP) from Saccharomyces cerevisiae...
  44. ncbi Regulation of early T cell development by the PHD finger of histone lysine methyltransferase ASH1
    Yujiro Tanaka
    Genome Structure and Expression, School of Biomedical Science, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyoku, Tokyo 113 8610, Japan
    Biochem Biophys Res Commun 365:589-94. 2008
    We have previously isolated a mammalian homologue of Drosophila discsabsent, small, orhomeotic-1 (ash1) from the murine thymus, and recently shown that its SET domain methylates histone H3 lysine 36 (K36)...
  45. doi Association of trxG and PcG proteins with the bxd maintenance element depends on transcriptional activity
    Svetlana Petruk
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Development 135:2383-90. 2008
    ..Among several trxG and PcG proteins, only Ash1 and Asx require Trithorax in order to bind to their target genes...
  46. pmc Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA
    Tung Gia Du
    Gene Center, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, D 81377 Munich, Germany
    EMBO Rep 9:781-7. 2008
    ..Here, we show that a specific transcript, known as ASH1 mRNA, and She2 localize specifically to the nucleolus when their nuclear export is blocked...
  47. pmc dSet1 is the main H3K4 di- and tri-methyltransferase throughout Drosophila development
    Graham Hallson
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Genetics 190:91-100. 2012
    ..but not in animals carrying mutations in genes encoding other well-characterized H3K4 HMTs such as trr, trx, and ash1. The latter results suggest that Trr, Trx, and Ash1 may play more specific roles in regulating key cellular targets ..
  48. doi miR-375 is activated by ASH1 and inhibits YAP1 in a lineage-dependent manner in lung cancer
    Eri Nishikawa
    Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Cancer Res 71:6165-73. 2011
    ..The transcription factor ASH1/ASCL1 is a master regulator of pulmonary NE cell development that is involved in the pathogenesis of lung cancers ..
  49. pmc Daughter-specific transcription factors regulate cell size control in budding yeast
    Stefano Di Talia
    The Rockefeller University, New York, New York, USA
    PLoS Biol 7:e1000221. 2009
    ..smaller daughter cell, which transcribe different genes due to the daughter-specific transcription factors Ace2 and Ash1. Cell size control at the Start checkpoint has long been considered to be a main regulator of the length of the G1 ..
  50. doi Imaging real-time gene expression in living yeast
    Daniel Zenklusen
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    CSH Protoc 2007:pdb.prot4870. 2007
    INTRODUCTIONThis protocol describes the application of the MS2 system to the yeast Saccharomyces cerevisiae. ASH1 mRNA tagged with six MS2 repeats (6MBSs) is used to follow the localization of the ASH1 mRNA particles to the bud tip of a ..
  51. ncbi Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast
    Naoko Mauchi
    Department of Molecular Cell Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, Tennoudai, Tsukuba, Japan
    Cell Struct Funct 35:95-105. 2010
    ..Khd1p associates with hundreds of potential mRNA targets including a bud-localized ASH1 mRNA and mRNAs encoding membrane-associated proteins such as Mid2p and Mtl1p...
  52. pmc Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae
    Sunglan Chung
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 189:755-67. 2010
    In Saccharomyces cerevisiae, ASH1 mRNA is transported to the bud tip by the class V myosin Myo4...
  53. pmc The ASH1 HOMOLOG 2 (ASHH2) histone H3 methyltransferase is required for ovule and anther development in Arabidopsis
    Paul E Grini
    Department of Molecular Biosciences, University of Oslo, Oslo, Norway
    PLoS ONE 4:e7817. 2009
    ..The Arabidopsis ASH1 HOMOLOG 2 (ASHH2) protein (also called SDG8, EFS and CCR1) has been suggested to methylate H3K4 and/or H3K36 and is ..
  54. doi Neurogenesis in an annelid: characterization of brain neural precursors in the polychaete Capitella sp. I
    Néva P Meyer
    Kewalo Marine Laboratory, Pacific Biosciences Research Center, University of Hawaii, Honolulu, HI 96813, USA
    Dev Biol 335:237-52. 2009
    ..Prior to and during this process, an achaete-scute complex homolog, CapI-ash1, is expressed in clusters of surface cells in the anterior ectoderm, consistent with a proneural function for CapI-..
  55. pmc Essential features of the class V myosin from budding yeast for ASH1 mRNA transport
    Carol S Bookwalter
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA
    Mol Biol Cell 20:3414-21. 2009
    ..Here, we determine the features of the Myo4p motor that are necessary for correct localization of ASH1 mRNA to the daughter cell, a process that also requires the adapter protein She3p and the dimeric mRNA-binding ..
  56. doi Phylotypic expression of the bHLH genes Neurogenin2, Neurod, and Mash1 in the mouse embryonic forebrain
    Joana Osorio
    CNRS, Institute of Neurobiology A Fessard, Development, Evolution, Plasticity of the Nervous System, Research Unit 2197, 91198 Gif sur Yvette, France
    J Comp Neurol 518:851-71. 2010
    ..Neurogenins/NeuroD on the one hand and Ascl1/ash1 on the other hand exhibit essentially mutually exclusive spatial patterns, probably reflecting different positional ..
  57. pmc Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p
    Zhifa Shen
    Departement de Biochimie, Universite de Montreal, Canada
    Mol Biol Cell 20:2265-75. 2009
    ..In yeast, the nucleocytoplasmic shuttling protein She2 binds the ASH1 mRNA and targets it for localization at the bud tip by recruiting the She3p-Myo4p complex...
  58. pmc Proneural gene ash1 promotes amacrine cell production in the chick retina
    Weiming Mao
    Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Dev Neurobiol 69:88-104. 2009
    ..To better understand how neural diversity is achieved during retinal neurogenesis, we examined the function of ash1, a proneural bHLH gene expressed in progenitor cells throughout retinal neurogenesis...
  59. pmc Systematic identification of yeast cell cycle transcription factors using multiple data sources
    Wei Sheng Wu
    Department of Evolution and Ecology, University of Chicago, Chicago, IL 60637, USA
    BMC Bioinformatics 9:522. 2008
    ..To understand the cell cycle process, it is important to identify the cell cycle transcription factors (TFs) that regulate the expression of cell cycle-regulated genes...
  60. pmc Drosophila Kismet regulates histone H3 lysine 27 methylation and early elongation by RNA polymerase II
    Shrividhya Srinivasan
    Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA, USA
    PLoS Genet 4:e1000217. 2008
    ..or function might be regulated by the methylation of histone H3 lysine 4 by the trithorax group proteins ASH1 and TRX...
  61. pmc Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiae
    Wei Zheng
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 180:1391-405. 2008
    ..In Saccharomyces cerevisiae, wild-type ASH1 mRNA is one of several dozen transcripts that are exported from the mother-cell nucleus during mitotic anaphase, ..
  62. pmc Conserved chromosomal clustering of genes governed by chromatin regulators in Drosophila
    Enrique Blanco
    Departament de Genètica and Institut de Biomedicina de la Universitat de Barcelona, Universitat de Barcelona, Barcelona, Catalonia, Spain
    Genome Biol 9:R134. 2008
    ..They bind to specific regions of DNA and direct the post-translational modifications of histones, playing a role in the dynamics of chromatin structure...
  63. pmc Dual function of histone H3 lysine 36 methyltransferase ASH1 in regulation of Hox gene expression
    Yujiro Tanaka
    Genome Structure and Regulation, School of Biomedical Science and Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
    PLoS ONE 6:e28171. 2011
    Hox genes play important roles in haematopoietic development in mammals. ASH1 is a member of the trithorax group (trxG) that is required for proper expression of Hox genes and is preferentially expressed in haematopoietic stem cells...
  64. ncbi Trans-regulation of thoracic homeotic selector genes of the Antennapedia and bithorax complexes by the trithorax group genes: absent, small, and homeotic discs 1 and 2
    D Lajeunesse
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Mech Dev 53:123-39. 1995
    ..According to genetic criteria, the Drosophila melanogaster genes absent, small, or homeotic discs 1 and 2 (ash1 and ash2) are members of the trithorax group...
  65. pmc She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae
    Hirofumi Toi
    Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine, Japan
    Mol Biol Cell 14:2237-49. 2003
    ..protein family, is required for endocytosis, polarization of actin cytoskeleton, and polarization of ASH1 mRNA in Saccharomyces cerevisiae...
  66. pmc Molecular genetic analysis of the Drosophila melanogaster gene absent, small or homeotic discs1 (ash1)
    N A Tripoulas
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218
    Genetics 137:1027-38. 1994
    The absent, small or homeotic discs1 gene (ash1) is one of the trithorax set of genes...
  67. pmc The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes
    L O Baumbusch
    Division of Molecular Biology, Department of Biology, University of Oslo, PO Box 1031 Blindern, N 0315 Norway
    Nucleic Acids Res 29:4319-33. 2001
    ..These proteins can be divided into four classes as typified by their Drosophila members E(Z), TRX, ASH1 and SU(VAR)3-9. Homologs of all four classes have been identified in yeast and mammals, but not in plants...
  68. pmc High-frequency generation of conditional mutations affecting Drosophila melanogaster development and life span
    G Landis
    Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 1340, USA
    Genetics 158:1167-76. 2001
    ..Mutations characterized included inserts in the alpha-mannosidase II (dGMII), ash1, and pumilio genes...
  69. pmc Pre-mRNA processing factors are required for nuclear export
    A S Brodsky
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    RNA 6:1737-49. 2000
    ..Moreover, when export is blocked, only RNAs containing the ASH1 3' UTR accumulated in the nucleolus...
  70. pmc Sok2 regulates yeast pseudohyphal differentiation via a transcription factor cascade that regulates cell-cell adhesion
    X Pan
    Departments of Genetics, Pharmacology and Cancer Biology, Microbiology, and Medicine, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 20:8364-72. 2000
    ..By genome array and Northern analysis, we found that genes encoding the transcription factors Phd1, Ash1, and Swi5 were all induced in sok2/sok2 hyperfilamentous mutants...
  71. pmc She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p
    F Bohl
    ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    EMBO J 19:5514-24. 2000
    RNA localization is a widespread mechanism to achieve localized protein synthesis. In budding yeast, localization of ASH1 mRNA controls daughter cell-specific accumulation of the transcriptional regulator Ash1p, which determines mating ..
  72. ncbi The PWWP domain: a potential protein-protein interaction domain in nuclear proteins influencing differentiation?
    I Stec
    MGC Department of Human and Clinical Genetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands
    FEBS Lett 473:1-5. 2000
    ..a gene mapping to the Wolf-Hirschhorn syndrome critical region and at its C-terminus similar to the Drosophila ASH1/trithorax group proteins, we identified a novel protein domain designated PWWP domain...
  73. ncbi Unconventional myosins and the genetics of hearing loss
    T B Friedman
    Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, Rockville, MD 20854, USA
    Am J Med Genet 89:147-57. 1999
    ..The discovery that the mouse shaker1 (Myo7(ash1)) locus encodes myosin VIIA led immediately to the identification of mutations in this gene in Usher syndrome type ..
  74. ncbi Self-association of the SET domains of human ALL-1 and of Drosophila TRITHORAX and ASH1 proteins
    T Rozovskaia
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel
    Oncogene 19:351-7. 2000
    ..and in vitro 'pull down' techniques to show self association of the SET motifs of ALL-1, TRITHORAX and ASH1 proteins (Drosophila ASH1 is encoded by a trithorax-group gene)...
  75. ncbi The bromodomain protein LIN-49 and trithorax-related protein LIN-59 affect development and gene expression in Caenorhabditis elegans
    H M Chamberlin
    Department of Genetics, University of Washington, Seattle, WA 98195 7360, USA
    Development 127:713-23. 2000
    ..most similar to the human bromodomain protein BR140, and LIN-59 is most similar to the Drosophila trx-G protein ASH1. In C...
  76. pmc Trithorax and ASH1 interact directly and associate with the trithorax group-responsive bxd region of the Ultrabithorax promoter
    T Rozovskaia
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel
    Mol Cell Biol 19:6441-7. 1999
    Trithorax (TRX) and ASH1 belong to the trithorax group (trxG) of transcriptional activator proteins, which maintains homeotic gene expression during Drosophila development...
  77. ncbi Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins
    S Münchow
    ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    J Cell Sci 112:1511-8. 1999
    ..Myo4p is required for the localisation of ASH1 mRNA, encoding a transcriptional repressor that is essential for differential expression of the HO gene and mating ..
  78. ncbi Exon trapping and sequence-based methods of gene finding in transcript mapping of human 4p16.3
    I Pribill
    Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown 02129, USA
    Somat Cell Mol Genet 23:413-27. 1997
    ..and several anonymous transcripts, including a putative novel DNA polymerase and a gene related to Drosophila ash1. While most of the genes in the region have been found, our data suggest that even with the entire DNA sequence ..
  79. pmc Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae
    S Chandarlapaty
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599 7260, USA
    Mol Cell Biol 18:2884-91. 1998
    b>Ash1 (for asymmetric synthesis of HO) was first uncovered in genetic screens that revealed its role in mating-type switching. Ash1 prevents HO expression in daughter cells...
  80. ncbi Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast
    P A Takizawa
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143 0450, USA
    Nature 389:90-3. 1997
    ..The protein Ash1 is a cell-fate determinant in budding yeast which localizes preferentially to the presumptive daughter nucleus, ..
  81. pmc Molecular genetic analysis of Drosophila ash2, a member of the trithorax group required for imaginal disc pattern formation
    A L Adamson
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genetics 144:621-33. 1996
    ..called a PHD finger, that is present not only in the products of other trithorax group genes such as TRX and ASH1, but also in the product of a Polycomb group gene, PCL...
  82. pmc Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae
    Paula Estrada
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA
    J Cell Biol 163:1255-66. 2003
    Myo4p is a nonessential type V myosin required for the bud tip localization of ASH1 and IST2 mRNA. These mRNAs associate with Myo4p via the She2p and She3p proteins. She3p is an adaptor protein that links Myo4p to its cargo...
  83. pmc A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization
    Wei Gu
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 18:1452-65. 2004
    ..The accumulation of Ash1p results from ASH1 mRNA that is sorted as a ribonucleoprotein particle (mRNP or locasome) to the distal tip of the bud where ..
  84. ncbi ASH1 gene is a specific therapeutic target for lung cancers with neuroendocrine features
    Hirotaka Osada
    Division of Molecular Oncology, Aichi Cancer Center Research Institute, Chikusa, Nagoya, Japan
    Cancer Res 65:10680-5. 2005
    ..The basic helix-loop-helix protein, achaete-scute complex-like 1/achaete-scute homologue 1 (ASH1), is expressed in normal fetal pulmonary neuroendocrine cells and lung cancers with neuroendocrine elements and is ..
  85. ncbi Multigene real-time PCR detection of circulating tumor cells in peripheral blood of lung cancer patients
    Dawn C Hayes
    GSK Biologicals Seattle, 1900 9th Avenue, Suite 1100, Seattle, WA 98101 USA
    Anticancer Res 26:1567-75. 2006
    CLCA2, HMGB3, L587S and ASH1 were identified in lung cancer tissues using genetic subtraction, microarray and quantitative PCR, and found to be specific and complementary for detection of non-small cell lung carcinoma (NSCLC) and small ..
  86. ncbi The scaleless wings mutant in Bombyx mori is associated with a lack of scale precursor cell differentiation followed by excessive apoptosis
    Qing Xiang Zhou
    Biotechnology Research Institute, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, People s Republic of China
    Dev Genes Evol 216:721-6. 2006
    ..In addition, we have studied Bm-ASH1, an achaete-scute homolog in B...
  87. pmc Daughter-specific repression of Saccharomyces cerevisiae HO: Ash1 is the commander
    Maria Pia Cosma
    Telethon Institute of Genetics and Medicine, Via P Castellino 111, 80131 Naples, Italy
    EMBO Rep 5:953-7. 2004
    The GATA-1-like factor Ash1 is a repressor of the HO gene, which encodes an endonuclease that is responsible for mating-type switching in the yeast Saccharomyces cerevisiae...
  88. ncbi Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex
    Michael J Carrozza
    Stowers Institute for Medical Research1000 East 50th Street, Kansas City, MO 64110, USA
    Biochim Biophys Acta 1731:77-87; discussion 75-6. 2005
    Histone deacetylation by Saccharomyces cerevisiae Rpd3 represses genes regulated by the Ash1 and Ume6 DNA-binding proteins. Rpd3 exists in a small 0.6 MDa (Rpd3S) and large 1.2 MDa (Rpd3L) corepressor complex...
  89. ncbi Coordination of endoplasmic reticulum and mRNA localization to the yeast bud
    Maria Schmid
    Department of Chemistry and Biochemistry, Gene Center, Ludwig Maximilians University Munich, D 81377 Munich, Germany
    Curr Biol 16:1538-43. 2006
    ..These proteins mediate the localization of 23 mRNAs including ASH1 mRNA and mRNAs encoding membrane proteins...
  90. ncbi RNA localization in yeast: moving towards a mechanism
    Graydon B Gonsalvez
    Department of Genetics, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Biol Cell 97:75-86. 2005
    ..The mechanism by which the mRNAs are trafficked to the bud, illustrated by ASH1 mRNA, involves recognition of cis-acting localization elements present in the mRNA by the RNA-binding protein, ..
  91. ncbi Virulence properties of motile aeromonads isolated from farmed frogs Rana tigerina and R. rugulosa
    M D Pearson
    Institute of Aquaculture, University of Stirling, Scotland, UK
    Dis Aquat Organ 40:185-93. 2000
    ..schubertii) were noted. DNA colony hybridisation studies revealed that Au isolates possessed a haemolysin gene (ASH1) which was not present in any of the other Thai aeromonads or type strains tested...
  92. pmc SCFCdc4 enables mating type switching in yeast by cyclin-dependent kinase-mediated elimination of the Ash1 transcriptional repressor
    Qingquan Liu
    School of Biological Sciences, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, United Kingdom
    Mol Cell Biol 31:584-98. 2011
    ..which initiates the interconversion of a and α mating type cassettes, is extinguished by the daughter-specific Ash1 transcriptional repressor...
  93. pmc A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae
    Erin A Powrie
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    RNA 17:134-44. 2011
    ..Importantly, downstream processes were also affected. Localization of ASH1 and IST2 mRNAs to the bud was impaired in the Δnup60 background, suggesting that the assembly of a localization ..
  94. ncbi Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast
    V Measday
    Department of Molecular and Medical Genetics, University of Toronto, Rm 4285 Medical Sciences Building, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8
    Mol Microbiol 35:825-34. 2000
    ..by Swi5 and a homologous transcription factor, Ace2, was misregulated in a pho85 deletion strain; expression of the ASH1 and CTS1 genes was reduced in an ace2 deletion strain, whereas expression of both genes was increased in an ..
  95. pmc A cytoplasmic complex mediates specific mRNA recognition and localization in yeast
    Marisa Müller
    Institute of Structural Biology, Helmholtz Zentrum Munchen German Research Center for Environmental Health, Munchen, Germany
    PLoS Biol 9:e1000611. 2011
    ..We used ASH1-mRNA transport in yeast to provide a first assessment of where and how localizing mRNAs are specifically recognized ..
  96. doi Bi-polarized translation of ascidian maternal mRNA determinant pem-1 associated with regulators of the translation machinery on cortical Endoplasmic Reticulum (cER)
    Alexandre Paix
    Université Pierre et Marie Curie and Centre National de la Recherche Scientifique, BioMarCell, UMR BioDev, Observatoire Oceanologique, Villefranche sur Mer, France
    Dev Biol 357:211-26. 2011
    ..cortical mRNA determinants such as maternal macho-1 and pem-1 in ascidians, like budding yeast mating factor ASH1 reside on the cER-mRNA domain a subdomain of cortical Endoplasmic Reticulum(ER) and are translated in its vicinity...
  97. pmc Repressive chromatin affects factor binding at yeast HO (homothallic switching) promoter
    Shinya Takahata
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, USA
    J Biol Chem 286:34809-19. 2011
    ..The Rpd3(L) histone deacetylase is recruited to HO at two distinct times during the cell cycle, first by Ash1 to the URS1 region of the promoter and then by SBF/Whi5/Stb1 to URS2...
  98. pmc SWI/SNF binding to the HO promoter requires histone acetylation and stimulates TATA-binding protein recruitment
    Doyel Mitra
    Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84132 2501, USA
    Mol Cell Biol 26:4095-110. 2006
    ..results showed that SWI/SNF binding to HO was Gcn5 independent, but that work used a strain with a mutation in the Ash1 daughter-specific repressor of HO expression...
  99. ncbi Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells
    N Bobola
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 84:699-709. 1996
    ..We describe a gene, ASH1, that is necessary to repress HO in daughters...
  100. ncbi Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene
    A Sil
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94143 0448 USA
    Cell 84:711-22. 1996
    ..We have isolated a mutant in which daughters transcribe HO and switch mating type. This mutation defines the ASH1 gene (asymmetric synthesis of HO)...
  101. pmc The Drosophila ash1 gene product, which is localized at specific sites on polytene chromosomes, contains a SET domain and a PHD finger
    N Tripoulas
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genetics 143:913-28. 1996
    ..The stability of these patterns requires the function of the ash1 gene, a member of the trithorax group. The primary translation product of the 7...

Research Grants66

  1. Mechanism of mRNA transport by Myo4p
    Peter Takizawa; Fiscal Year: 2009
    ..To address these questions, this proposal will analyze how Myo4p transports ASH1 mRNA to the bud tip in S. cerevisiae...
  2. Cell-fate determinants of yeast pseudohyphal growth
    Beverly Errede; Fiscal Year: 2005
    ..While the mechanism responsible for asymmetric localization of Ash1 has been elucidated, very little is known about the daughter-specific program that it mediates to establish the PH ..
  3. Biochemistry and Structure of RNA-Protein Complexes
    James Williamson; Fiscal Year: 2006
    ..RNA-protein complex involved in localization and translational regulation of the Saccharomyces cerevesiae ASH1 mRNA. ASH1 is localized to the tip of a budding daughter cell, and the ASH1 protein regulates mating type switching...
  4. An E3 Ubiquitin Ligase Complex That Regulates C. elegans Developmental Timing
    Adam Antebi; Fiscal Year: 2009
    ..3. characterize putative targets of DRE-1, including the trithorax protein LIN- 59/ASH1. lin-59 mutant defects, genetic interactions, expression pattern and regulation will be determined...
  5. An E3 Ubiquitin Ligase Complex That Regulates C. elegans Developmental Timing
    Adam Antebi; Fiscal Year: 2007
    ..3. characterize putative targets of DRE-1, including the trithorax protein LIN- 59/ASH1. lin-59 mutant defects, genetic interactions, expression pattern and regulation will be determined...
  6. The switch between epigenetically silenced and active chromatin states
    Vincenzo Pirrotta; Fiscal Year: 2010
    ..Trithorax and ASH1 proteins are necessary to switch genes to an epigenetic active state and to antagonize Polycomb silencing...
  7. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert H Singer; Fiscal Year: 2010
    ..yeast Saccharomyces cerevisiae, mating type switching is tightly controlled by the asymmetric accumulation of the ASH1 gene product...
  8. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2007
    ..yeast Saccharomyces cerevisiae, mating type switching is tightly controlled by the asymmetric accumulation of the ASH1 gene product...
  9. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2006
    ..The synthesis ofbeta-actin at the leading edge facilitates cell polarity and motility. In yeast, ASH1 mRNA localizes to the bud tip and is necessary for asymmetric gene expression between the mother and the bud, ..
  10. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2003
    ..The synthesis ofbeta-actin at the leading edge facilitates cell polarity and motility. In yeast, ASH1 mRNA localizes to the bud tip and is necessary for asymmetric gene expression between the mother and the bud, ..
  11. MOLECULAR BASIS OF DROSOPHILA HOMEOTIC GENE REGULATION
    Peter J Harte; Fiscal Year: 2010
    ..including involvement in reversing Polycomb silencing at the onset of differentiation 3) investigate whether ASH1 also mediates H3K27 acetylation through its known association with CBP...
  12. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2001
    ..I. proposes a series of aims designed to address the localization of ASH1 mRNA in yeast cells...
  13. A NEURAL TRANSCRIPTION FACTOR IN LUNG CANCER EVOLUTION
    Douglas Ball; Fiscal Year: 2003
    ..In mammalian development, ASH1 (termed MASH1 in rodents) is essential for normal differentiation of sympathoadrenal, enteric, olfactory, retinal, ..
  14. A Molecular Basis for Neuroendocrine Carcinogenesis
    H Grimes; Fiscal Year: 2007
    ..oncoprotein and the basic helix-loop-helix (bHLH) transcription factor human achaete and scute homolog- 1 (ASH1) are modifiers of SCLC because they control neuroendocrine differentiation of lung epithelium...
  15. A Molecular Basis for Neuroendocrine Carcinogenesis
    H Grimes; Fiscal Year: 2009
    ..oncoprotein and the basic helix-loop-helix (bHLH) transcription factor human achaete and scute homolog- 1 (ASH1) are modifiers of SCLC because they control neuroendocrine differentiation of lung epithelium...
  16. RNA LOCALIZATION IN S.CEREVISIAE
    ROY LONG; Fiscal Year: 2004
    ..The yeast S. cerevisiae serves as a model system for studying the asymmetric segregation of mRNA. ASH1 mRNA is localized to the distal end of daughter cells during anaphase of the cell cycle, resulting in the exclusive ..
  17. Genetic and Molecular Studies of Drosophila Chromatin Remodeling Factors
    JOHN TAMKUN; Fiscal Year: 2009
    ..Our studies of chromatin-remodeling factors in a genetic model organism should therefore shed light on the molecular mechanisms underlying these human cancers and other diseases. ..
  18. GENETIC/BIOCHEM STUDIES OF CHROMATIN REMODELING FACTORS
    JOHN TAMKUN; Fiscal Year: 2000
    ..The ability to conduct genetic studies of chromatin remodeling factors in Drosophila thus represents an excellent opportunity to increase our understanding of their roles in transcription, development and disease. ..
  19. Studies of Chromatin Remodeling Factors
    JOHN TAMKUN; Fiscal Year: 2004
    ..Thus, the information gained from the study of chromatin remodeling factors in Drosophila should be directly relevant to human disease. ..
  20. Genetic and Molecular Studies of Drosophila Chromatin Remodeling Factors
    JOHN WALTER TAMKUN; Fiscal Year: 2010
    ..The information gained from this project will therefore be directly relevant to human health. ..
  21. Molecular-genetic analysis of the Drosophila CNS midline
    SCOTT WHEELER; Fiscal Year: 2007
    ..Through the combination of descriptive and functional-genetic studies I will begin to dissect the molecular and genetic pathways that control the development and differentiation midline neurons. ..
  22. Genetic and Molecular Studies of Drosophila Chromatin Remodeling Factors
    JOHN TAMKUN; Fiscal Year: 2007
    ..Our studies of chromatin-remodeling factors in a genetic model organism should therefore shed light on the molecular mechanisms underlying these human cancers and other diseases. ..
  23. REGULATION OF GENE EXPRESSION DURING ORGANOGENESIS
    Helen Chamberlin; Fiscal Year: 2004
    ..elegans. A third aim is to functionally characterize this second gene to determine whether its product shares in vivo functions with EGL-38, or whether it regulates lin-48 and cdh-3 expression in other organs. ..