ARHGEF2

Summary

Gene Symbol: ARHGEF2
Description: Rho/Rac guanine nucleotide exchange factor (GEF) 2
Alias: GEF, GEF-H1, GEFH1, LFP40, P40, Rho/Rac guanine nucleotide exchange factor 2, guanine nucleotide exchange factor H1, microtubule-regulated Rho-GEF, proliferating cell nucleolar antigen p40, rho guanine nucleotide exchange factor 2
Species: human

Top Publications

  1. ncbi p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor
    Frank T Zenke
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:18392-400. 2004
  2. ncbi Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases
    Y Ren
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 273:34954-60. 1998
  3. ncbi Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton
    Mira Krendel
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Cell Biol 4:294-301. 2002
  4. ncbi Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition
    Saima Aijaz
    Division of Cell Biology, Institute of Ophthalmology, University College London, UK
    Dev Cell 8:777-86. 2005
  5. ncbi ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function
    M Franco
    Centre d Immunologie Institut National de la Santé et de la Recherche Médicale Centre National de la Recherche Scientifique de Marseille Luminy, Case 906, 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 95:9926-31. 1998
  6. ncbi Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution
    W Feng
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    J Cell Sci 112:339-47. 1999
  7. ncbi Perturbations in the spi1p GTPase cycle of Schizosaccharomyces pombe through its GTPase-activating protein and guanine nucleotide exchange factor components result in similar phenotypic consequences
    A Matynia
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 16:6352-62. 1996
  8. ncbi RCC1 in the Ran pathway
    T Seki
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka
    J Biochem 120:207-14. 1996
  9. ncbi Two ras pathways in fission yeast are differentially regulated by two ras guanine nucleotide exchange factors
    Piyi Papadaki
    Biology Department, New York University, New York, New York 10003 6688, USA
    Mol Cell Biol 22:4598-606. 2002
  10. ncbi Quantitative morphology and postsynaptic targets of thalamocortical axons in critical period and adult ferret visual cortex
    Alev Erisir
    University of Virginia, Department of Psychology, Charlottesville, Virginia 22904 4400, USA
    J Comp Neurol 485:11-31. 2005

Research Grants

  1. Mechanism of Modulation of Interleukin-12 Production by Triptolide
    Xiaojing Ma; Fiscal Year: 2007
  2. ACTIVATION OF NF-KB IN GLIAL CELLS AND CNS OF EAE
    Kalipada Pahan; Fiscal Year: 2009
  3. MOLECULAR REGULATION OF INTERLEUKIN 12 GENE EXPRESSION
    Xiaojing Ma; Fiscal Year: 2003
  4. Diverse Roles for RIC8 During G Protein Signaling in Fungi
    Katherine A Borkovich; Fiscal Year: 2010
  5. Mechanisms of action IL-23 in relapsing demyelination
    Abdolmohamad Rostami; Fiscal Year: 2007
  6. Pairing of EBI-3 and IL12 p35 To Yield A Novel Regulatory T cell Cytokine
    LAUREN COLLISON; Fiscal Year: 2007
  7. Regulation of IL-12 Expression and Activity by Oncogenes
    Xiaojing Ma; Fiscal Year: 2007
  8. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Gary Bokoch; Fiscal Year: 2000
  9. Structural Determinants of Heterotrimeric G-protein Nucleotide Cycling
    DAVID SIDEROVSKI; Fiscal Year: 2009
  10. Structural Determinants of Heterotrimeric G-protein Nucleotide Cycling
    David P Siderovski; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications135 found, 100 shown here

  1. ncbi p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor
    Frank T Zenke
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:18392-400. 2004
    b>GEF-H1 is a guanine nucleotide exchange factor for Rho whose activity is regulated through a cycle of microtubule binding and release...
  2. ncbi Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases
    Y Ren
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 273:34954-60. 1998
    ..The Dbl-like guanine nucleotide exchange factors (GEF) have been implicated in direct activation of these GTPases...
  3. ncbi Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton
    Mira Krendel
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Cell Biol 4:294-301. 2002
    ..Here we show that the Rho guanine nucleotide exchange factor (GEF)-H1 is regulated by an interaction with microtubules...
  4. ncbi Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition
    Saima Aijaz
    Division of Cell Biology, Institute of Ophthalmology, University College London, UK
    Dev Cell 8:777-86. 2005
    ..Here we show that GEF-H1/Lfc, a guanine nucleotide exchange factor for RhoA, directly interacts with cingulin, a junctional adaptor...
  5. ncbi ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function
    M Franco
    Centre d Immunologie Institut National de la Santé et de la Recherche Médicale Centre National de la Recherche Scientifique de Marseille Luminy, Case 906, 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 95:9926-31. 1998
    ..We recently have identified a human protein, ARNO (ARF nucleotide-binding-site opener), as an ARF1-GEF that shares a conserved domain with the yeast Sec7 protein...
  6. ncbi Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution
    W Feng
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    J Cell Sci 112:339-47. 1999
    ..In yeast Rna1p is the GTPase activating protein for Ran (RanGAP) and Prp20p is the Ran GDP/GTP exchange factor (GEF). RanGAP is primarily cytosolic and GEF is nuclear...
  7. ncbi Perturbations in the spi1p GTPase cycle of Schizosaccharomyces pombe through its GTPase-activating protein and guanine nucleotide exchange factor components result in similar phenotypic consequences
    A Matynia
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 16:6352-62. 1996
    ..products, rna1p and pim1p, functioning as GTPase-activating protein (GAP) and guanine nucleotide exchange factor (GEF), respectively...
  8. ncbi RCC1 in the Ran pathway
    T Seki
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka
    J Biochem 120:207-14. 1996
    RCC1 is a chromosomal protein that functions as a GEF of the nuclear G protein Ran, which GTPase activity is enhanced by RNA1 located in the cytoplasm...
  9. ncbi Two ras pathways in fission yeast are differentially regulated by two ras guanine nucleotide exchange factors
    Piyi Papadaki
    Biology Department, New York University, New York, New York 10003 6688, USA
    Mol Cell Biol 22:4598-606. 2002
    ..Ras1 activates Scd1, a presumptive guanine nucleotide exchange factor (GEF) for Cdc42, to control morphogenesis and chromosome segregation, and Byr2, a component of a mitogen-activated ..
  10. ncbi Quantitative morphology and postsynaptic targets of thalamocortical axons in critical period and adult ferret visual cortex
    Alev Erisir
    University of Virginia, Department of Psychology, Charlottesville, Virginia 22904 4400, USA
    J Comp Neurol 485:11-31. 2005
    ..anterograde tract-tracing display significantly larger cross-section areas than terminals at postnatal day (P) 35, P40, and P49 critical period ages...
  11. ncbi Interaction between 3' untranslated region of calcitonin receptor messenger ribonucleic acid (RNA) and adenylate/uridylate (AU)-rich element binding proteins (AU-rich RNA-binding factor 1 and Hu antigen R)
    Shigemitsu Yasuda
    Fourth Department of Internal Medicine, Saitama Medical School, Japan
    Endocrinology 145:1730-8. 2004
    ..3'UTR transcripts bound with a 40-kDa protein, which could be extracted from cells transfected with AUF1 p40. To confirm the binding specificity, a pSG5 vector construct, containing the AUF1 p40 with an hemagglutinin tag, ..
  12. ncbi Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream target of M-Ras
    X Gao
    Division of Molecular Biology, Department of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Japan
    J Biol Chem 276:42219-25. 2001
    ..RA-GEF-1 was identified as a novel Rap GEF, which possesses a Ras/Rap1-associating (RA) domain...
  13. ncbi Measles virus N protein inhibits host translation by binding to eIF3-p40
    Hiroki Sato
    Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Virol 81:11569-76. 2007
    ..by using MV-N as bait and screening the human T-cell cDNA library by yeast two-hybrid assay and isolated the p40 subunit of eukaryotic initiation factor 3 (eIF3-p40) as a binding partner...
  14. ncbi Increased circulating interleukin-12 (IL-12) p40 in pulmonary sarcoidosis
    K Shigehara
    First Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan
    Clin Exp Immunol 132:152-7. 2003
    ..The serum concentration of IL-12 p40 was detectable in all 45 patients with pulmonary sarcoidosis and 18 normal controls, whereas that of IL-12 p70 was ..
  15. ncbi Spinal dural arteriovenous malformations. Intraoperative evoked potential evidence for pathophysiology. A case report
    M M Stecker
    Department of Neurology, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania
    Spine (Phila Pa 1976) 21:512-5. 1996
    ..RESULTS. At baseline, the cortical (P40) potential was markedly prolonged bilaterally...
  16. ncbi In vivo clearance of an intracellular bacterium, Francisella tularensis LVS, is dependent on the p40 subunit of interleukin-12 (IL-12) but not on IL-12 p70
    Karen L Elkins
    Laboratory of Mycobacteria, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, Rockville, Maryland 20852, USA
    Infect Immun 70:1936-48. 2002
    ..Mice lacking the p40 protein of IL-12 (p40 knockout [KO] mice) and mice treated in vivo with neutralizing anti-IL-12 antibodies survived ..
  17. ncbi Mouse interleukin-12 (IL-12) p40 homodimer: a potent IL-12 antagonist
    S Gillessen
    Department of Inflammation Autoimmune Diseases, Hoffmann La Roche Inc, Nutley, NJ 07110
    Eur J Immunol 25:200-6. 1995
    ..that has regulatory effects on T and natural killer (NK) cells and is composed of two disulfide-bonded subunits, p40 and p35...
  18. ncbi Effects of stimulus intensity and duration on posterior tibial nerve somatosensory-evoked potential patterns obtained under anesthesia
    M Rappaport
    University of California Brain Function Study Unit, Agnews Developmental Center, San Jose
    Spine (Phila Pa 1976) 19:1525-9. 1994
    ..RESULTS. Three latencies yielded stable findings (P40, N50, P60). Peak latencies under anesthesia were not affected by stimulus intensity or duration...
  19. ncbi Effect of CYP2C19*2 and *17 mutations on pharmacodynamics and kinetics of proton pump inhibitors in Caucasians
    Nicole G Hunfeld
    Central Hospital Pharmacy, The Hague, The Netherlands
    Br J Clin Pharmacol 65:752-60. 2008
    ..Furthermore, a novel CYP2C19 mutation, *17, which mainly occurs in Caucasians has been discovered. This mutation has been associated with clinical failure, but its relevance for therapy with PPIs has not been studied yet...
  20. ncbi Role of Mycoplasma penetrans endonuclease P40 as a potential pathogenic determinant
    M Bendjennat
    Laboratory of Immunovirology UFR SVT, University of Paul Sabatier, 31062 Toulouse, France
    Infect Immun 67:4456-62. 1999
    ..we reported the purification to homogeneity and characterization of Ca(2+)- and Mg(2+)-dependent endonuclease P40 produced by Mycoplasma penetrans (M. Bendjennat, A. Blanchard, M. Loutfi, L. Montagnier, and E. Bahraoui, J...
  21. ncbi IL-12 and IFN-gamma production, and NK cell activity, in acute and chronic experimental Trypanosoma cruzi infections
    M I Antunez
    Instituto Nacional de Parasitologia Dr M Fatala Chaben, ANLIS Dr C G Malbran, Buenos Aires, Argentina
    Immunol Lett 71:103-9. 2000
    ..cruzi, Tulahuén strain was studied. The levels of interleukin-12 p40 (IL-12 p40) and IFN-gamma, as well as natural killer (NK) cell cytotoxicity were determined at different time-..
  22. ncbi Complementation of NADPH oxidase in p67-phox-deficient CGD patients p67-phox/p40-phox interaction
    S Vergnaud
    GREPI, Laboratoire d Enzymologie, CHU, Grenoble, France INSERM U 479, CHU, Bichat, Paris, France
    Eur J Biochem 267:1059-67. 2000
    ..b558 that was associated with cytosol or with the isolated cytosolic-activating factors (p67-phox, p47-phox, p40-phox) from healthy or CGD patients...
  23. ncbi Modulation of IL-12 p35 and p40 promoter activity by smokeless tobacco extract is associated with an effect upon activation of NF-kappaB but not IRF transcription factors
    Thomas M Petro
    Department of Oral Biology, College of Dentistry, University of Nebraska Medical Center, 40th and Holdrege Streets, Lincoln, NE 68583 0740, USA
    Int Immunopharmacol 3:735-45. 2003
    Interleukin (IL)-12 (p70), composed of p35 and p40 subunits, stimulates cellular immunity and inflammation. Stimulation of IL-12 production by smokeless tobacco extract (STE) could increase the chances of oral inflammatory disease...
  24. ncbi Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity
    P Ling
    Department of Inflammation Autoimmune Diseases, Hoffmann La Roche, Inc, Nutley, NJ 07110
    J Immunol 154:116-27. 1995
    IL-12, a heterodimeric cytokine, consists of two disulfide-linked subunits, p40 and p35. We investigated the role of p40 in ligand binding and signal transduction by expressing this subunit alone in COS cells...
  25. ncbi Lack of interleukin-12 in p40-deficient mice leads to poor CD8+ T-cell immunity against Encephalitozoon cuniculi infection
    Magali M Moretto
    Department of Microbiology, George Washington University, 2300 I Street, Washington, DC 20037, USA
    Infect Immun 78:2505-11. 2010
    ..In the present study, we demonstrated that p40-deficient animals, which are unable to produce interleukin-12 (IL-12), have a serious defect in expansion of the ..
  26. ncbi Eukaryotic initiation factor 2B (eIF2B) GEF activity as a diagnostic tool for EIF2B-related disorders
    Laetitia Horzinski
    INSERM U931 CNRS 6247 Université Clermont, GReD, Clermont Ferrand, France
    PLoS ONE 4:e8318. 2009
    ..Decreased eIF2B GEF activity has been previously reported in lymphoblastoid cell lines from 30 eIF2B-mutated patients...
  27. ncbi Dual system to reinforce biological containment of recombinant bacteria designed for rhizoremediation
    M C Ronchel
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    Appl Environ Microbiol 67:2649-56. 2001
    ..g., a porin-inducing protein such as the one encoded by the Escherichia coli gef gene, and a regulatory circuit that controls expression of the killing gene in response to the presence or absence ..
  28. ncbi The interleukin 12 p40 gene promoter is primed by interferon gamma in monocytic cells
    X Ma
    Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104, USA
    J Exp Med 183:147-57. 1996
    ..IL-12 production mostly by priming phagocytic cells for lipopolysaccharide (LPS)-induced transcription of the IL-12 p40 gene, which encodes the heavy chain of the IL-12 heterodimer; furthermore, IFN-gamma directly induces transcription ..
  29. ncbi PEST-dependent cytoplasmic retention of v-Rel by I(kappa)B-alpha: evidence that I(kappa)B-alpha regulates cellular localization of c-Rel and v-Rel by distinct mechanisms
    E M Rottjakob
    Department of Biochemistry, University of Missouri, Columbia 65212, USA
    J Virol 70:3176-88. 1996
    ..The ability of v-Rel, the oncogenic viral counterpart of avian c-Rel, to evade regulation by p40, the avian IkappaB-alpha protein, contributes to v-Rel-mediated oncogenesis...
  30. ncbi Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells
    A D'ANDREA
    Wistar Institute of Anatomy and Biology, Children s Hospital of Philadelphia, Pennsylvania 19104
    J Exp Med 176:1387-98. 1992
    ..NKSF), or interleukin 12 (IL-12), is a 70-kD heterodimeric cytokine composed of two covalently linked chains, p40 and p35...
  31. ncbi Estimation of left ventricular systolic function by single transpulmonary thermodilution
    Alain Combes
    Service de Reanimation Medicale, Hopital Pitie Salpetriere, Assistance Publique Hopitaux de Paris, 47, Boulevard de l Hopital, 75651 Paris Cedex 13, France
    Intensive Care Med 30:1377-83. 2004
    ..The global cardiac systolic function may be decreased despite preserved left ventricular function in this situation.)..
  32. ncbi Drosophila PDZ-GEF, a guanine nucleotide exchange factor for Rap1 GTPase, reveals a novel upstream regulatory mechanism in the mitogen-activated protein kinase signaling pathway
    Jun Hee Lee
    National Creative Research Initiatives Center for Cell Growth Regulation and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305 701, Korea
    Mol Cell Biol 22:7658-66. 2002
    PDZ-GEF is a novel guanine nucleotide exchange factor for Rap1 GTPase...
  33. ncbi Expression of the guanine nucleotide exchange factor, mr-gef, is regulated during the differentiation of specific subsets of telencephalic neurons
    Angela Bithell
    Section of Experimental Neuropathology and Psychiatry, Department of Psychological Medicine, The Institute of Psychiatry P052, De Crespigny Park, London SE5 8AF, UK
    Brain Res Dev Brain Res 146:107-18. 2003
    ..Here we report that one of these genes, encoding a guanine nucleotide exchange factor (GEF), is regulated during the differentiation of distinct neuronal populations...
  34. ncbi Induction of rac-guanine nucleotide exchange activity of Ras-GRF1/CDC25(Mm) following phosphorylation by the nonreceptor tyrosine kinase Src
    M Kiyono
    Faculty of Bioscience, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 275:5441-6. 2000
    Ras-GRF1/CDC25(Mm) has been implicated as a Ras-guanine nucleotide exchange factor (GEF) expressed in brain. Ras-GEF activity of Ras-GRF1 is augmented in response to Ca(2+) influx and G protein betagamma subunit (Gbetagamma) stimulation...
  35. ncbi Characterization and localized expression of the laminin binding protein/p40 (LBP/p40) gene during sea urchin development
    M Hung
    Department of Biological Sciences, California State University, Hayward 94542, USA
    Exp Cell Res 221:221-30. 1995
    ..of a cDNA clone from the sea urchin Strongylocentrotus purpuratus that encodes a protein very similar to LBP/p40, originally identified as a nonintegrin, 67-kDa laminin binding protein...
  36. ncbi RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing a Ras/Rap1A-associating domain, is conserved between nematode and humans
    Y Liao
    Department of Physiology II, Kobe University School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    J Biol Chem 274:37815-20. 1999
    ..for Ras-binding proteins in nematode Caenorhabditis elegans has identified a guanine nucleotide exchange factor (GEF) containing a Ras/Rap1A-associating (RA) domain, termed Ce-RA-GEF...
  37. ncbi Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells
    S F Wolf
    Genetics Institute, Inc, Cambridge, MA 02140
    J Immunol 146:3074-81. 1991
    ..NKSF was found to be a disulfide-linked heterodimeric protein composed of 35-kDa and 40-kDa subunits (p35 and p40). We now report the molecular cloning of cDNA for both subunits of NKSF from RPMI 8866 cellular RNA...
  38. ncbi Specific recognition of Rac2 and Cdc42 by DOCK2 and DOCK9 guanine nucleotide exchange factors
    Michael A Kwofie
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Biol Chem 283:3088-96. 2008
    Recognition of cognate Rho GTPases by guanine-nucleotide exchange factors (GEF) is fundamental to Rho GTPase signaling specificity...
  39. ncbi Stimulation of Ras guanine nucleotide exchange activity of Ras-GRF1/CDC25(Mm) upon tyrosine phosphorylation by the Cdc42-regulated kinase ACK1
    M Kiyono
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 275:29788-93. 2000
    Ras-GRF1 is a brain-specific guanine nucleotide exchange factor (GEF) for Ras, whose activity is regulated in response to Ca(2+) influx and G protein-coupled receptor signals...
  40. ncbi Induction of lamellipodia by Kalirin does not require its guanine nucleotide exchange factor activity
    Martin R Schiller
    Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Ave, Farmington, CT 06030 3401, USA
    Exp Cell Res 307:402-17. 2005
    Guanine nucleotide exchange factor (GEF) domains of the Dbl family occur in a variety of proteins that include multiple protein-protein and protein-lipid interaction domains...
  41. ncbi The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains
    A Debant
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 93:5466-71. 1996
    ..isolated a cDNA encoding a 2861-amino acid protein termed Trio that contains three enzyme domains: two functional GEF domains and a protein serine/threonine kinase (PSK) domain...
  42. ncbi cAMP-GEF cytoprotection by Src tyrosine kinase activation of phosphoinositide-3-kinase p110 beta/alpha in rat hepatocytes
    Anna Gates
    Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, 200 Westboro Rd, North Grafton, MA 01536, USA
    Am J Physiol Gastrointest Liver Physiol 296:G764-74. 2009
    Cyclic AMP protects against hepatocyte apoptosis by a protein kinase A-independent cAMP-GEF/phosphoinositide-3-kinase (PI3K)/Akt signaling pathway. However, the signaling pathway coupling cAMP-GEF with PI3K is unknown...
  43. ncbi The role of the conserved switch II glutamate in guanine nucleotide exchange factor-mediated nucleotide exchange of GTP-binding proteins
    Raphael Gasper
    Abteilung Strukturelle Biologie, Max Planck Institut fur molekulare Physiologie, 44227 Dortmund, Germany
    J Mol Biol 379:51-63. 2008
    ..The mechanism involves the formation of trimeric G protein-nucleotide-GEF complexes, followed by the release of nucleotide to form stable binary G protein-GEF complexes...
  44. ncbi Exhaustive exercise and type-1/type-2 cytokine balance with special focus on interleukin-12 p40/p70
    Katsuhiko Suzuki
    Department of Hygiene, Hirosaki University School of Medicine, Aomori, Japan
    Exerc Immunol Rev 9:48-57. 2003
    ..produce type-1 cytokines such as IL-2 and interferon (IFN), thereby supporting cellular immunity, whereas IL-12 p40 acts as an antagonist of IL-12 p70...
  45. ncbi betaPak-interacting exchange factor-mediated Rac1 activation requires smgGDS guanine nucleotide exchange factor in basic fibroblast growth factor-induced neurite outgrowth
    Eun Young Shin
    Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361 763, Korea
    J Biol Chem 281:35954-64. 2006
    ..1994-2004), we demonstrated that betaPak-interacting exchange factor (betaPIX) guanine nucleotide exchange factor (GEF) mediates basic fibroblast growth factor (bFGF)-stimulated Rac1 activation through phosphorylation of Ser-525 and ..
  46. ncbi Heparin inhibits pulmonary artery smooth muscle cell proliferation through guanine nucleotide exchange factor-H1/RhoA/Rho kinase/p27
    Lunyin Yu
    Pulmonary and Critical Care Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 2696, USA
    Am J Respir Cell Mol Biol 44:524-30. 2011
    ..We also investigated the effect of guanine nucleotide exchange factor (GEF)-H1, an upstream regulator of RhoA, on heparin inhibition of PASMC proliferation by GEF-H1 cDNA transfection...
  47. ncbi Inhibitors of ATP-binding cassette transporters suppress interleukin-12 p40 production and major histocompatibility complex II up-regulation in macrophages
    Gyorgy Hasko
    Department of Surgery, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey 07103, USA
    J Pharmacol Exp Ther 301:103-10. 2002
    ..in the regulation of lipopolysaccharide (LPS) and/or interferon (IFN)-gamma-induced interleukin (IL)-12 p40 and tumor necrosis factor (TNF)-alpha production, nitric oxide formation, as well as major histocompatibility ..
  48. ncbi Expression of the Rap1 guanine nucleotide exchange factor, MR-GEF, is altered in individuals with bipolar disorder
    Angela Bithell
    Department of Psychological Medicine, Institute of Psychiatry, King s College London, London, United Kingdom
    PLoS ONE 5:e10392. 2010
    ..The Rap-1 guanine nucleotide exchange factor, MR-GEF, is turned on by many of these developing GABAergic neurons...
  49. ncbi Guanine nucleotide exchange factor independence of the G-protein eEF1A through novel mutant forms and biochemical properties
    Sedide B Ozturk
    Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 283:23244-53. 2008
    Most G-proteins require a guanine nucleotide exchange factor (GEF) to regulate a variety of critical cellular processes. Interestingly, a small number of G-proteins switch between the active and inactive forms without a GEF...
  50. ncbi Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity
    D Dailey
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Mol Cell Biol 10:6244-56. 1990
    ..level of tyrosine kinase activity at all steps of each preparation correlated with the content of a 40-kDa protein (p40)...
  51. ncbi Regulation of interleukin-12 expression in human monocytes: selective priming by interferon-gamma of lipopolysaccharide-inducible p35 and p40 genes
    M P Hayes
    Division of Cytokine Biology, Food and Drug Administration, Bethesda, MD 20892, USA
    Blood 86:646-50. 1995
    ..factor (GM-CSF) provided an equivalent priming stimulus for LPS-induced tumor necrosis factor (TNF) and IL-12 p40 mRNA, but primed poorly for LPS-inducible p35 message and secreted IL-12 activity...
  52. ncbi Protective immunity induced with 23 kDa membrane protein dna vaccine of Schistosoma japonicum Chinese strain in infected C57BL/6 mice
    Yingchang Zhu
    Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, PR China
    Southeast Asian J Trop Med Public Health 34:697-701. 2003
    ..m.) with a mixture of 100 microg of pcDNA3.1-SjC23, 100 microg of pcDNA3.1-p35 and 100 microg of pcDNA-p40. These were followed by two boosts of the same DNA once every two weeks...
  53. ncbi Induction of tumor necrosis factor-alpha (TNF-alpha) by interleukin-12 p40 monomer and homodimer in microglia and macrophages
    Malabendu Jana
    Department of Oral Biology, University of Nebraska Medical Center, Lincoln, Nebraska 68583 0740, USA
    J Neurochem 86:519-28. 2003
    The present study was undertaken to explore the role of interleukin-12 (IL-12) p40 in the expression of TNF-alpha in microglia...
  54. ncbi Effect of granulocyte colony-stimulating factor on IL-12 p40 production during chemotherapy for B-cell lineage non-Hodgkin's lymphoma patients
    Tomomi Toubai
    Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Eur J Haematol 77:403-9. 2006
    Interleukin (IL)-12 is a 70-kDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response...
  55. ncbi Characterization of p40 and IL-10 in the BALF of patients with pulmonary sarcoidosis
    Virginie Barbarin
    Industrial Toxicology and Occupational Medicine Unit, Universite Catholique de Louvain, Brussels, Belgium
    J Interferon Cytokine Res 23:449-56. 2003
    This study investigated cytokine protein levels (interleukin-12 p70 [IL-12p70], p40, and IL-10) in bronchoalveolar lavage fluid (BALF) from patients with pulmonary sarcoidosis (n = 59), healthy control subjects (n = 17), and patients ..
  56. ncbi Induction of lymphotoxin-alpha by interleukin-12 p40 homodimer, the so-called biologically inactive molecule, but not IL-12 p70
    Malabendu Jana
    Department of Neurological Sciences, Rush University Medical Center, Chicago, IL 60612, USA
    Immunology 127:312-25. 2009
    Interleukin-12 (IL-12) p70 (p40:p35) is a bioactive cytokine and its biological functions are becoming clear...
  57. ncbi Do subplate neurons comprise a transient population of cells in developing neocortex of rats?
    R T Robertson
    Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine, California 92697 1280, USA
    J Comp Neurol 426:632-50. 2000
    ..dissector method was used to analyze fluorescently stained material in animals the age of postnatal day 0 (P0) to P40. These results demonstrate a reduction of slightly less than half of the total number of subplate neurons from P0 ..
  58. ncbi Ovarian follicular concentration of IL-12, IL-15, IL-18 and p40 subunit of IL-12 and IL-23
    Sanja Vujisic
    Department of Obstetrics and Gynecology, Medical School, University of Zagreb, Sveti Duh Hospital, Croatia
    Hum Reprod 21:2650-5. 2006
    The aim of the study was to determine the presence of interleukin (IL)-12, IL-15, IL-18 and p40 subunit of IL-12/IL-23 in follicular fluid from spontaneous cycles and the relation between the concentration of selected cytokines and IVF-..
  59. ncbi Copper induces the assembly of a multiprotein aggregate implicated in the release of fibroblast growth factor 1 in response to stress
    M Landriscina
    Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074, USA
    J Biol Chem 276:25549-57. 2001
    ..is known to be released in response to stress conditions as a component of a multiprotein aggregate containing the p40 extravescicular domain of p65 synaptotagmin (Syt) 1 and S100A13...
  60. ncbi Repeated subcutaneous injections of IL12/23 p40 neutralising antibody, ustekinumab, in patients with relapsing-remitting multiple sclerosis: a phase II, double-blind, placebo-controlled, randomised, dose-ranging study
    Benjamin M Segal
    Department of Neurology, Holtom Garrett Programme in Neuroimmunology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Lancet Neurol 7:796-804. 2008
    Repeated subcutaneous injections of a monoclonal antibody against the p40 subunit of interleukins 12 and 23, ustekinumab, were used to treat patients with relapsing-remitting multiple sclerosis (RRMS) to assess the drug's safety, ..
  61. ncbi Generation of an active protein-tyrosine kinase from lymphocytes by proteolysis
    T F Zioncheck
    Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, Indiana 47907
    J Biol Chem 263:19195-202. 1988
    ..found in soluble thymus extracts, as measured by the phosphorylation of angiotensin I, is a 40-kDa enzyme known as p40 (Zioncheck, T. F., Harrison, M. L., and Geahlen, R. L. (1986) J. Biol. Chem. 261, 15637-15643)...
  62. ncbi Nitric oxide inhibits interleukin-12 p40 through p38 MAPK-mediated regulation of calmodulin and c-rel
    Chandra Sekhar Boddupalli
    Laboratory of Molecular and Cellular Biology, Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076, Andhra Pradesh, India
    Free Radic Biol Med 42:686-97. 2007
    ..macrophages, the rel/NF-kappaB transcription factors are known to play important roles in interleukin-12 (IL-12) p40 regulation by nitric oxide (NO). However, the relative contributions of these factors are not well understood...
  63. ncbi Accumulation of processing intermediates of the RAS2 protein in strain 112 of Saccharomyces cerevisiae
    D Breviario
    Istituto Tecnologie Biomediche Avanzate, CNR, Milano, Italy
    Biochem Biophys Res Commun 151:1346-51. 1988
    ..molecule of 41000 daltons (p41), 112 cells accumulate within the soluble fraction an intermediate form of RAS2 (p40-1), which migrates, in SDS-polyacrylamide gel, between p41 and the fully processed, membrane-bound 40,000 daltons (..
  64. ncbi The Rab21-GEF activity of Varp, but not its Rab32/38 effector function, is required for dendrite formation in melanocytes
    Norihiko Ohbayashi
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Mol Biol Cell 23:669-78. 2012
    ..Although Varp contains a Rab21-guanine nucleotide exchange factor (GEF) domain (i.e...
  65. ncbi PIN polarity regulation by AGC-3 kinases and ARF-GEF: a recurrent theme with context dependent modifications for plant development and response
    Pankaj Dhonukshe
    Department of Biology, Utrecht University, Utrecht, The Netherlands
    Plant Signal Behav 6:1333-7. 2011
    ..findings suggest that two categories of molecules, namely AGC-3 kinase family members PINOID, WAG1, WAG2 and ARF-GEF family member GNOM predominantly influence the polar localization of PINs...
  66. ncbi Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex
    S Zhan
    Pediatric Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 88:2714-21. 1996
    b>p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox...
  67. ncbi IL-12 p40 homodimer, but not IL-12 p70, induces the expression of IL-16 in microglia and macrophages
    Malabendu Jana
    Department of Neurological Sciences, Rush University Medical Center, Cohn Research Building, Suite 320, 1735 West Harrison St, Chicago, IL 60612, United States
    Mol Immunol 46:773-83. 2009
    ..The present study underlines the importance of IL-12 p40 homodimer (p40(2)), the so-called biologically inactive molecule, in inducing the expression of IL-16 in primary ..
  68. ncbi Diagnostic value of interleukin-12 p40 in tuberculous pleural effusions
    L Valdes
    Pulmonology Service, Complejo Hospitalario Clínico Universitario de Santiago, Santiago de Compostela, Spain
    Eur Respir J 33:816-20. 2009
    ..deaminase (ADA), ADA isoform ADA-2, interferon (IFN)-gamma, CD3(+)/DR(+) T-lymphocytes and interleukin (IL)-12 p40 were determined in all 96 effusions...
  69. ncbi Interleukin-12 production by dendritic cells. The role of CD40-CD40L interactions in Th1 T-cell responses
    B L Kelsall
    Mucosal Immunity Section, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 795:116-26. 1996
    ..Initially we showed that FACS-purified mouse spleen DCs produce high amounts of IL-12 p40 in response to CD40 crosslinking by CD40L-expressing fibroblasts...
  70. ncbi Translocation of guinea pig p40-phox during activation of NADPH oxidase
    A Someya
    Department of Biochemistry, Juntendo University, School of Medicine, Tokyo, Japan
    Biochim Biophys Acta 1277:217-25. 1996
    ..Recently, we have found a novel cytosolic component, p40-phox, which is tightly associated with p67-phox...
  71. ncbi The structure of interleukin-23 reveals the molecular basis of p40 subunit sharing with interleukin-12
    Patrick J Lupardus
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Mol Biol 382:931-41. 2008
    ..IL-23 is a binary complex of a four-helix bundle cytokine (p19) and a soluble class I cytokine receptor p40. IL-12 and IL-23 share p40 as an alpha-receptor subunit, yet show only 15% sequence homology between their four-..
  72. ncbi Regulation of Kalirin by Cdk5
    Xiaonan Xin
    Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3401, USA
    J Cell Sci 121:2601-11. 2008
    ..Expression of Kalirin-7 in nondifferentiated PC12 cells caused GEF-activity-dependent extension of broad cytoplasmic protrusions; coexpression of dominant-negative Cdk5 largely ..
  73. ncbi Treatment with homodimeric interleukin-12 (IL-12) p40 protects mice from IL-12-dependent shock but not from tumor necrosis factor alpha-dependent shock
    F Mattner
    Department of Infectious Diseases, F Hoffmann La Roche AG, Basel, Switzerland
    Infect Immun 65:4734-7. 1997
    ..IL-12 is composed of two disulfide-bonded subunits, p35 and p40. The IL-12 p40 homodimer (p40)2 has been shown to be a potent IL-12 antagonist in vitro...
  74. ncbi Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex
    D H Presky
    Department of Inflammation Autoimmune Diseases, Hoffmann La Roche Inc, Nutley, NJ 07110, USA
    J Immunol 160:2174-9. 1998
    IL-12 is a heterodimeric cytokine, composed of a p40 and a p35 subunit, that exerts its biological effects by binding to specific cell surface receptors...
  75. ncbi Role of cytokine p40 family in multiple sclerosis
    S Brahmachari
    Department of Neurological Sciences, Rush University Medical Center, Chicago, IL 60612, USA
    Minerva Med 99:105-18. 2008
    Over the last couple of decades of neuro-immunological research, the p40 family of cytokines has emerged out as one of the most intriguing areas of interest because of multi-faceted roles of these cytokine in immune-modulation and ..
  76. ncbi GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA
    Yuan Chen Chang
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 19:2147-53. 2008
    ..b>GEF-H1 is a microtubule-associated guanine nucleotide exchange factor that activates RhoA upon release from ..
  77. ncbi GLUT4 enhancer factor (GEF) interacts with MEF2A and HDAC5 to regulate the GLUT4 promoter in adipocytes
    David P Sparling
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Biol Chem 283:7429-37. 2008
    ..MEF2 proteins and GLUT4 enhancer factor (GEF) are known ligands for these cis-acting elements, but their mechanism of action is unclear...
  78. ncbi Detection of borna disease virus-reactive antibodies from patients with psychiatric disorders and from horses by electrochemiluminescence immunoassay
    K Yamaguchi
    Blood Transfusion Service and Internal Medicine, Kumamoto University School of Medicine, Kumamoto, Japan
    Clin Diagn Lab Immunol 6:696-700. 1999
    ..new electrochemiluminescence immunoassay (ECLIA) for the antibody to BDV that uses two recombinant proteins of BDV, p40 and p24 (full length)...
  79. ncbi Circulating IL-12 p40 is increased in the patients with sarcoidosis, correlation with clinical markers
    Masahiro Hata
    Third Department of Internal Medicine, Division of Pulmonary Disease, Oita University Faculty of Medicine
    Intern Med 46:1387-93. 2007
    ..Because interleukin 12 (IL-12) is one of the most important cytokines for promoting Th1 reaction, we tried to detect IL-12 and its ligand IL-12 receptor in sarcoidosis patients...
  80. ncbi GEF-H1 mediates tumor necrosis factor-alpha-induced Rho activation and myosin phosphorylation: role in the regulation of tubular paracellular permeability
    Eli Kakiashvili
    Keenan Research Centre in the Li Ka Shing Knowledge Institute, St Michael s Hospital and Department of Surgery, University of Toronto, Ontario M5B 1W8, Canada
    J Biol Chem 284:11454-66. 2009
    ..Mass spectrometry analysis of the RhoG17A-precipitated proteins identified GEF-H1 as a TNF-alpha-activated Rho GEF...
  81. ncbi Regulation of GLUT4 expression in denervated skeletal muscle
    Ellis B Jensen
    Department of Biology, Viterbo University, La Crosse, Wisconsin, USA
    Am J Physiol Regul Integr Comp Physiol 296:R1820-8. 2009
    ..to decreased activation of the transcription factors myocyte enhancer factor 2 (MEF2) and GLUT4 enhancer factor (GEF), which are required for normal GLUT4 expression...
  82. ncbi The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins
    Christophe Guilluy
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 13:722-7. 2011
    ..biochemical and biophysical approaches, we identified two guanine nucleotide exchange factors (GEFs), LARG and GEF-H1, as key molecules that regulate the cellular adaptation to force...
  83. ncbi cAMP-guanine exchange factor protection from bile acid-induced hepatocyte apoptosis involves glycogen synthase kinase regulation of c-Jun NH2-terminal kinase
    A Johnston
    Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, Grafton, Massachusetts 01536, USA
    Am J Physiol Gastrointest Liver Physiol 301:G385-400. 2011
    ..Increases in cAMP protect hepatocytes from bile acid-induced apoptosis by a cAMP-guanine exchange factor (cAMP-GEF)/phosphoinositide-3-kinase (PI3K)/Akt pathway...
  84. ncbi A minimal Rac activation domain in the unconventional guanine nucleotide exchange factor Dock180
    Xin Wu
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States
    Biochemistry 50:1070-80. 2011
    ..Two metazoan families of GEFs have been described: Dbl-GEF family members that share conserved Dbl homology (DH) and Pleckstrin homology (PH) domains and the more recently ..
  85. ncbi Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells
    Takuji Tsuji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Mol Biol Cell 21:3590-600. 2010
    ..WGEF, a member of the Rho-guanine nucleotide exchange factor (Rho-GEF) family, was shown to play a role in Wnt-induced RhoA activation in Xenopus embryos...
  86. ncbi Knockout of p47 phox uncovers a critical role of p40 phox in reactive oxygen species production in microvascular endothelial cells
    Lampson M Fan
    Faculty of Health and Medical Sciences, AY Building, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Arterioscler Thromb Vasc Biol 29:1651-6. 2009
    b>p40(phox) is an important regulatory subunit of NADPH oxidase, but its role in endothelial reactive oxygen species (ROS) production remains unknown.
  87. ncbi Structural basis for the dual recognition of IL-12 and IL-23 by ustekinumab
    Jinquan Luo
    Centocor Research and Development, Inc, 145 King of Prussia Road, Radnor, PA 19087, USA
    J Mol Biol 402:797-812. 2010
    Interleukin (IL)-12 and IL-23 are heterodimeric proinflammatory cytokines that share a common p40 subunit, paired with p35 and p19 subunits, respectively...
  88. ncbi Human P40/IL-9. Expression in activated CD4+ T cells, genomic organization, and comparison with the mouse gene
    J C Renauld
    Unit of Experimental Medicine, Universite Catholique de Louvain, Belgium
    J Immunol 144:4235-41. 1990
    b>P40 is a cytokine that was originally identified in the mouse as a T cell growth factor, but whose spectrum of potential targets was recently shown to include mast cells as well as megakaryoblastic leukemic cells...
  89. ncbi The Drosophila stubarista phenotype is associated with a dosage effect of the putative ribosome-associated protein D-p40 on spineless
    M B Melnick
    Department of Genetics, Harvard Medical School, Boston Massachusetts 02115
    Genetics 135:553-64. 1993
    ..melanogaster gene stubarista (sta) that encodes the highly conserved putative ribosome-associated protein D-p40. sta maps to cytological position 2A3-B2 on the X chromosome and encodes a protein (D-p40) of 270 amino acids...
  90. ncbi Extracellular signal-regulated kinase and GEF-H1 mediate depolarization-induced Rho activation and paracellular permeability increase
    Faiza Waheed
    St Michael s Hospital, Toronto, ON, Canada
    Am J Physiol Cell Physiol 298:C1376-87. 2010
    ..Searching for the underlying mechanism, we have identified the GTP/GDP exchange factor GEF-H1 as the ERK-regulated critical exchange factor responsible for the depolarization-induced Rho activation...
  91. ncbi Arabidopsis p40 homologue. A novel acidic protein associated with the 40 S subunit of ribosomes
    M Garcia-Hernandez
    Department of Agronomy, University of Nebraska, Lincoln 68583 0915
    J Biol Chem 269:20744-9. 1994
    We have isolated a full-length cDNA clone from Arabidopsis thaliana that has extensive homology to p40 proteins from other organisms. The cDNA predicts a protein (A-p40) of 298 amino acids with a calculated molecular mass of 32...
  92. ncbi Semaphorin 4D signaling requires the recruitment of phospholipase C gamma into the plexin-B1 receptor complex
    Jakub M Swiercz
    Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D 69120 Heidelberg, Germany
    Mol Cell Biol 29:6321-34. 2009
    ....
  93. ncbi An interleukin 12 p40-IgG2b fusion protein abrogates T cell mediated inflammation: anti-inflammatory activity in Crohn's disease and experimental colitis in vivo
    A Stallmach
    Department of Internal Medicine II, Saarland University, Homburg, Germany
    Gut 53:339-45. 2004
    Interleukin-12 (IL-12), a p35/p40 heterodimer, plays a pivotal role in the immune response in Crohn's disease (CD). Since IL-12 p40 dimers act as IL-12 antagonists, we assayed p40 dimer proteins to modulate chronic intestinal inflammation.
  94. ncbi Suppression of regulatory T cells by IL-12p40 homodimer via nitric oxide
    Saurav Brahmachari
    Department of Neurological Sciences, Rush University Medical Center, Chicago, IL 60612, USA
    J Immunol 183:2045-58. 2009
    ..Because the IL-12p40 homodimer (p40(2)) is markedly up-regulated in response to various inflammatory stimuli, the present study was undertaken to ..
  95. ncbi Characterisation of the nucleotide exchange factor ITSN1L: evidence for a kinetic discrimination of GEF-stimulated nucleotide release from Cdc42
    Carsten Kintscher
    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
    J Mol Biol 387:270-83. 2009
    ..of the Dbl-homology domain containing constructs of the adaptor protein Intersectin1L (ITSN1L), which is a specific GEF for Cdc42...
  96. ncbi Purification and characterization of an alpha-actinin-binding PDZ-LIM protein that is up-regulated during muscle differentiation
    P Pomies
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Biol Chem 274:29242-50. 1999
    ..Interestingly, in skeletal muscle, a closely related protein of 40 kDa (p40) is detected...
  97. ncbi The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes
    Yoko Yoshikawa
    Division of Molecular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Mol Biol Cell 18:2949-59. 2007
    ..However, the mechanism for Rap1 regulation in the adhesion signaling remains unclear. RA-GEF-2 is a member of the multiple-member family of guanine nucleotide exchange factors (GEFs) for Rap1 and ..
  98. ncbi Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophages
    Qi Luo
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:18451-6. 2004
    Transcription of interleukin (IL)-12 p40 in myeloid cells is attributed to the recruitment of multiple activated transcription factors such as nuclear factor kappaB (NFkappaB), CCAAT enhancer-binding protein beta, ets-2, PU...
  99. ncbi Intracellular localization of p40, a protein identified in a preparation of lysosomal membranes
    Marielle Boonen
    URPhiM, Laboratoire de Chimie Physiologique, Facultes Universitaires Notre Dame de la Paix, B 5000 Namur, Belgium
    Biochem J 395:39-47. 2006
    ..We focused our attention on a protein of approx. 40 kDa, p40, which contains seven to ten putative transmembrane domains and four lysosomal consensus sorting motifs in its ..
  100. ncbi Regulation of muscle GLUT4 enhancer factor and myocyte enhancer factor 2 by AMP-activated protein kinase
    Burton F Holmes
    Department of Exercise and Sport Science, Human Performance Laboratory, Brody School of Medicine, East Carolina University, Greenville, NC, USA
    Am J Physiol Endocrinol Metab 289:E1071-6. 2005
    ..GLUT4 enhancer factor (GEF) and myocyte enhancer factor 2 (MEF2) have been shown to be important for normal GLUT4 expression because deletion ..
  101. ncbi Molecular cloning and functional characterization of guinea pig IL-12
    I Shiratori
    Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari ku, Osaka 537 8511, Japan
    Int Immunol 13:1129-39. 2001
    ..We cloned complete cDNAs of guinea pig homologues of IL-12 p35 and p40 subunits, and compared their functional properties with human IL-12...

Research Grants86

  1. Mechanism of Modulation of Interleukin-12 Production by Triptolide
    Xiaojing Ma; Fiscal Year: 2007
    Interleukin-12 (IL-12) is a heterodimeric cytokine composed of the p40 and p35 chains...
  2. ACTIVATION OF NF-KB IN GLIAL CELLS AND CNS OF EAE
    Kalipada Pahan; Fiscal Year: 2009
    ..We have discovered a new group of inducers of NF-kappaB activation [interleukin-12 (IL-12) p40 homodimer (p40-2) and monomer (p40)] in glial cells. It was known that p40-2 and p40 were biologically inactive...
  3. MOLECULAR REGULATION OF INTERLEUKIN 12 GENE EXPRESSION
    Xiaojing Ma; Fiscal Year: 2003
    ..IL-12 is a heterodimer consisted to two subunits, p40 and p35 that are encoded on different human chromosomes...
  4. Diverse Roles for RIC8 During G Protein Signaling in Fungi
    Katherine A Borkovich; Fiscal Year: 2010
    ..Loss of ric8 leads to reduced levels of G proteins in Drosophila, suggesting a chaperone function in addition to GEF activity. RIC8 is absent from plants, protists and the yeast Saccharomyces cerevisiae...
  5. Mechanisms of action IL-23 in relapsing demyelination
    Abdolmohamad Rostami; Fiscal Year: 2007
    Project 3. The Role of IL-23 in CNS Inflammatory DemyelinaUon. We have shown that antibodies against the p40 subunit of interleukin-12 (IL-12) prevent spontaneous and superantigen-induced relapses of experimental autoimmune ..
  6. Pairing of EBI-3 and IL12 p35 To Yield A Novel Regulatory T cell Cytokine
    LAUREN COLLISON; Fiscal Year: 2007
    Epstein barr virus induced gene 3 (EBI-3) is a soluble protein that shares structural similarities to the IL12 p40 chain...
  7. Regulation of IL-12 Expression and Activity by Oncogenes
    Xiaojing Ma; Fiscal Year: 2007
    ..The genes encoding the two heterologous chains of IL-12, p40 and p35 are located on different chromosomes. Together, p40 and p35 form the biologically active IL-12...
  8. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Gary Bokoch; Fiscal Year: 2000
    ..Translocation to the membrane requires nucleotide exchange by a membrane- bound exchange factor (GEF), and is accompanied by release from a cytosolic complex with Guanine dissociation inhibitors (GDI(s)) that ..
  9. Structural Determinants of Heterotrimeric G-protein Nucleotide Cycling
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ..This guanine nucleotide exchange factor (GEF) activity of GPCRs is the initial step in the G-protein cycle and determines the onset of various intracellular ..
  10. Structural Determinants of Heterotrimeric G-protein Nucleotide Cycling
    David P Siderovski; Fiscal Year: 2010
    ..This guanine nucleotide exchange factor (GEF) activity of GPCRs is the initial step in the G-protein cycle and determines the onset of various intracellular ..
  11. Structural Determinants of Heterotrimeric G-protein Nucleotide Cycling
    David P Siderovski; Fiscal Year: 2010
    ..This guanine nucleotide exchange factor (GEF) activity of GPCRs is the initial step in the G-protein cycle and determines the onset of various intracellular ..
  12. ELMO1, Dock180 and Glioma Invasion
    Shi Yuan Cheng; Fiscal Year: 2010
    ..binding partner, dedicator of cytokinesis 1 (Dock180) function as a bipartite guanine nucleotide exchange factor (GEF) that activates Rac1 and promotes cell migration of C. elegans, Drosophila and mammalian cells...
  13. Novel Molecules in Calcium Signaling in Platelets
    Wolfgang Bergmeier; Fiscal Year: 2010
    ..CD-GEF proteins are guanine nucleotide exchange factors for Ras family small GTPases...
  14. Control of Renal Glutaminase Expression during Acidosis
    Norman P Curthoys; Fiscal Year: 2010
    ..HuR and p40-AUF1 bind to the pHRE with high affinity and specificity...
  15. Control of Renal Glutaminase Expression during Acidosis
    Norman Curthoys; Fiscal Year: 2009
    ..HuR and p40-AUF1 bind to the pHRE with high affinity and specificity...
  16. Control of Renal Glutaminase Expression during Acidosis
    Norman Curthoys; Fiscal Year: 2009
    ..HuR and p40-AUF1 bind to the pHRE with high affinity and specificity...
  17. Regulators of Translation Elongation Factor eEF1A
    Terri Goss Kinzy; Fiscal Year: 2010
    ..eEF1A requires the guanine nucleotide exchange factor (GEF) eEF1Ba? for reactivation to the GTP-bound form...
  18. Regulators of Translation Elongation Factor eEF1A
    TERRI KINZY; Fiscal Year: 2009
    ..eEF1A requires the guanine nucleotide exchange factor (GEF) eEF1Ba? for reactivation to the GTP-bound form...
  19. Targeting the Fn14-Rac1 signaling pathway in invasive gliomas
    Nhan Tran; Fiscal Year: 2009
    ..We found that Ect2, a guanine nucleotide exchange factor (GEF), is overexpressed in GBM, and that overexpression of both Ect2 and Fn14 is correlated directly with tumor grade ..
  20. Targeting the Fn14-Rac1 signaling pathway in invasive gliomas
    Nhan L Tran; Fiscal Year: 2010
    ..We found that Ect2, a guanine nucleotide exchange factor (GEF), is overexpressed in GBM, and that overexpression of both Ect2 and Fn14 is correlated directly with tumor grade ..
  21. Dissecting the role of one neuronal RhoGEF amongst many: the Kalirin-7 null mouse
    Richard E Mains; Fiscal Year: 2010
    ..Deficits in expression of Kalirin, a large, dual Rho GEF protein, are associated with increased iNOS levels in Alzheimer's disease brain and with decreased spine ..
  22. DBL-LIKE REGULATORS OF SMALL G-PROTEINS
    Yi Zheng; Fiscal Year: 2000
    ..The dbl oncogene product is a cytoskeletal-associated protein functioning as guanine nucleotide exchange factor (GEF) for Rho-type small GTP-binding proteins...
  23. Selective control of multiple Ras signaling activities
    Eric Chang; Fiscal Year: 2007
    ..It is not known how a given Ras protein is networked with a given GEF and a proper effector...
  24. SIGNALING VIA THE MULTIFUNCTIONAL TRIO PROTEIN
    Haruo Saito; Fiscal Year: 2001
    ..The multifunctional Trio protein contains a rac1-specific GEF domain, a rhoA-specific GEF domain, a protein serine\threonine kinase domain, and a number of auxiliary domains...
  25. RHOA EFFECTOR IN HIV-1 REPLICATION AND PATHOGENESIS
    Lishan Su; Fiscal Year: 2004
    ..gp41 (gp41C) interacts with and inhibits p115-RhoGEF, which is a specific guanine nucleotide exchange factor (GEF) and activator of RhoA...
  26. Novel Mechanisms Regulating the Heterotrimeric G Protein Complex
    Alan M Jones; Fiscal Year: 2010
    ..G protein cycle contains several interesting properties, namely activation of a G1 subunit that does not require a GEF, regulation of the cycle at the GTP hydrolysis step, and a 7TM protein that may be the ligand-regulated GAP ..
  27. Novel Mechanisms Regulating the Heterotrimeric G Protein Complex
    Alan Jones; Fiscal Year: 2009
    ..G protein cycle contains several interesting properties, namely activation of a G1 subunit that does not require a GEF, regulation of the cycle at the GTP hydrolysis step, and a 7TM protein that may be the ligand-regulated GAP ..
  28. Probiotics-derived soluble proteins regulate intestinal inflammation
    Fang Yan; Fiscal Year: 2010
    ..We have successfully purified and cloned two novel LGG-derived soluble proteins (p40 and p75) that prevent cytokine-induced apoptosis through activating Akt in intestinal epithelial cells...
  29. NOVEL SECOND MESSENGER SIGNALING IN THE STRIATUM
    ANN GRAYBIEL; Fiscal Year: 2003
    ..Many Ras GEFs, but until the work described, only Rap GEF, have been identified...
  30. BABOON RESEARCH RESOURCE PROGRAM
    Gary White; Fiscal Year: 2007
    This P40 competitive renewal proposes to continue the aims of the initial grant application and expand the support of research investigators using baboons as models in biomedical and behavioral research...
  31. Viper Resource Center (VRC) at Texas A&M University-Kingsville
    John Perez; Fiscal Year: 2009
    The Viper Resource Center (VRC), P40 competitive renewal, at Texas A&M University-Kingsville proposes to continue the aims of the initial grant application...
  32. Viper Resource Center (VRC) at Texas A&M University-Kingsville
    JOHN CARLOS PEREZ; Fiscal Year: 2010
    The Viper Resource Center (VRC), P40 competitive renewal, at Texas A&M University-Kingsville proposes to continue the aims of the initial grant application...
  33. Viper Resource Center (VRC) at Texas A&M University-Kingsville
    John Perez; Fiscal Year: 2009
    The Viper Resource Center (VRC), P40 competitive renewal, at Texas A&M University-Kingsville proposes to continue the aims of the initial grant application...
  34. FUNCTION OF THE VAV PROTOONCOGENE
    Arthur Weiss; Fiscal Year: 2004
    ..The Vav family of proteins contains multiple modular domains including a guanine nucleotide exchange factor (GEF) domain that can regulate small GTPases of the Rho family...
  35. ROLE OF IL-23 IN PSORIASIS PATHOGENESIS
    Andrew Blauvelt; Fiscal Year: 2007
    ..IL-23, a recently discovered cytokine that consists of p19 and p40 subunits, is now believed to be critical in the development of several classic autoimmune inflammatory diseases in ..