Gene Symbol: AMSH
Description: STAM binding protein
Alias: AMSH, MICCAP, STAM-binding protein, associated molecule with the SH3 domain of STAM, endosome-associated ubiquitin isopeptidase, testicular secretory protein Li 54
Species: human
Products:     AMSH

Top Publications

  1. Agromayor M, Martin Serrano J. Interaction of AMSH with ESCRT-III and deubiquitination of endosomal cargo. J Biol Chem. 2006;281:23083-91 pubmed
    ..Here we report the interaction of AMSH (associated molecule with the SH3 domain of STAM), an endosomal deubiquitinating enzyme, with the endodomal sorting complex required for ..
  2. Ma Y, Boucrot E, Villen J, Affar E, Gygi S, Gottlinger H, et al. Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation. J Biol Chem. 2007;282:9805-12 pubmed
    ..Here we report that CHMP3, an ESCRT-III complex component, and associated molecule of SH3 domain of STAM (AMSH), a deubiquitinating enzyme, interact with each other in cells...
  3. Ambroggio X, Rees D, Deshaies R. JAMM: a metalloprotease-like zinc site in the proteasome and signalosome. PLoS Biol. 2004;2:E2 pubmed publisher
    ..The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors...
  4. Kyuuma M, Kikuchi K, Kojima K, Sugawara Y, Sato M, Mano N, et al. AMSH, an ESCRT-III associated enzyme, deubiquitinates cargo on MVB/late endosomes. Cell Struct Funct. 2007;31:159-72 pubmed
    ..Ubiquitinated cargo is targeted to endosomes and digested by lysosomal enzymes. We previously identified AMSH, a deubiquitination enzyme (DUB), to be involved in vesicular transport...
  5. Sierra M, Wright M, Nash P. AMSH interacts with ESCRT-0 to regulate the stability and trafficking of CXCR4. J Biol Chem. 2010;285:13990-4004 pubmed publisher
    ..The deubiquitinating enzyme AMSH has been implicated in the endocytic sorting of both G protein-coupled receptors and receptor-tyrosine kinases...
  6. McCullough J, Row P, Lorenzo O, Doherty M, Beynon R, Clague M, et al. Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery. Curr Biol. 2006;16:160-5 pubmed
    b>AMSH is an endosomal ubiquitin isopeptidase that can limit EGF receptor downregulation . It directly binds to the SH3 domain of STAM, which is constitutively associated with Hrs, a component of clathrin-coated structures on endosomes...
  7. Tanaka N, Kaneko K, Asao H, Kasai H, Endo Y, Fujita T, et al. Possible involvement of a novel STAM-associated molecule "AMSH" in intracellular signal transduction mediated by cytokines. J Biol Chem. 1999;274:19129-35 pubmed
    ..cloned a novel molecule interacting with the SH3 domain of STAM, which was named AMSH (associated molecule with the SH3 domain of STAM)...
  8. Kim M, Kim J, Song H, Jeon H. STAM-AMSH interaction facilitates the deubiquitination activity in the C-terminal AMSH. Biochem Biophys Res Commun. 2006;351:612-8 pubmed
    ..Associated molecule with SH3 domain of STAM (AMSH), a zinc-containing ubiquitin isopeptidase, is thought to play a role in regulation of ubiquitin-mediated ..
  9. Wright M, Berlin I, Nash P. Regulation of endocytic sorting by ESCRT-DUB-mediated deubiquitination. Cell Biochem Biophys. 2011;60:39-46 pubmed publisher
    ..Two dubiquitinating enzymes--ubiquitin-specific protease 8 (USP8/Ubpy) and associated molecule with the SH3 domain of STAM (AMSH)--interact with ESCRT components to modulate the ubiquitination status of receptors and ..

More Information


  1. Itoh F, Asao H, Sugamura K, Heldin C, ten Dijke P, Itoh S. Promoting bone morphogenetic protein signaling through negative regulation of inhibitory Smads. EMBO J. 2001;20:4132-42 pubmed
    ..Here we report the identification of a cytoplasmic protein, previously termed associated molecule with the SH3 domain of STAM (AMSH), as a direct binding partner for Smad6...
  2. Maytal Kivity V, Reis N, Hofmann K, Glickman M. MPN+, a putative catalytic motif found in a subset of MPN domain proteins from eukaryotes and prokaryotes, is critical for Rpn11 function. BMC Biochem. 2002;3:28 pubmed
    ..The putative catalytic nature of the MPN+ motif makes it a good candidate for a pivotal enzymatic function, possibly a proteasome-associated deubiquitinating activity and a CSN-associated Nedd8/Rub1-removing activity. ..
  3. Kato M, Miyazawa K, Kitamura N. A deubiquitinating enzyme UBPY interacts with the Src homology 3 domain of Hrs-binding protein via a novel binding motif PX(V/I)(D/N)RXXKP. J Biol Chem. 2000;275:37481-7 pubmed
    ..Ubiquitination of growth factor receptors is thought to regulate their intracellular degradation. Thus, UBPY may play a regulatory role in the degradation by interaction with the SH3 domain of Hbp via the novel SH3-binding motif. ..
  4. Ishii N, Owada Y, Yamada M, Miura S, Murata K, Asao H, et al. Loss of neurons in the hippocampus and cerebral cortex of AMSH-deficient mice. Mol Cell Biol. 2001;21:8626-37 pubmed
    b>AMSH, a molecule that associates with STAM1, is involved in the in vitro cell growth signaling mediated by interleukin 2 and granulocyte-macrophage colony-stimulating factor...
  5. Huang F, Zeng X, Kim W, Balasubramani M, Fortian A, Gygi S, et al. Lysine 63-linked polyubiquitination is required for EGF receptor degradation. Proc Natl Acad Sci U S A. 2013;110:15722-7 pubmed publisher
    ..and post-endocytic sorting of EGFR, a chimeric protein, in which the K63 linkage-specific deubiquitination enzyme AMSH [associated molecule with the Src homology 3 domain of signal transducing adaptor molecule (STAM)] was fused to the ..
  6. Hurley J. Nipped in the bud: how the AMSH MIT domain helps deubiquitinate lysosome-bound cargo. Structure. 2011;19:1033-5 pubmed publisher
    Recruitment of the K63-linkage specific deubiquitinating enzyme AMSH is an important step in ESCRT-dependent membrane protein sorting. In this issue of Structure, Solomons et al...
  7. Hori I, Miya F, Negishi Y, Hattori A, Ando N, Boroevich K, et al. A novel homozygous missense mutation in the SH3-binding motif of STAMBP causing microcephaly-capillary malformation syndrome. J Hum Genet. 2018;63:957-963 pubmed publisher
  8. Agromayor M, Carlton J, Phelan J, Matthews D, Carlin L, Ameer Beg S, et al. Essential role of hIST1 in cytokinesis. Mol Biol Cell. 2009;20:1374-87 pubmed publisher
    ..that is necessary for its interaction with VPS4, LIP5 and other MIT domain-containing proteins, namely, MITD1, AMSH, UBPY, and Spastin...
  9. Ding G, Zou W, Zhang H, Xue Y, Cai Y, Huang G, et al. In vivo tactile stimulation-evoked responses in Caenorhabditis elegans amphid sheath glia. PLoS ONE. 2015;10:e0117114 pubmed publisher
    ..elegans amphid sheath glia (AMsh glia) to tactile stimulation in vivo...
  10. Birol M, Echalier A. Structure and function of MPN (Mpr1/Pad1 N-terminal) domain-containing proteins. Curr Protein Pept Sci. 2014;15:504-17 pubmed
    ..incorporated in a large multi-protein complex have also been reported and include AMSH (for associated molecule with the SH3 domain of STAM) and the AMSH-Like Protein (AMSH-LP)...
  11. Govil M, Mukhopadhyay N, Weeks D, Feingold E, Shaffer J, Levy S, et al. Novel caries loci in children and adults implicated by genome-wide analysis of families. BMC Oral Health. 2018;18:98 pubmed publisher
    ..Understanding the genetic etiology of dental caries will allow designing personalized treatment plans based on an individual's genetic risk of disease. ..
  12. Pavlovic M, Neubauer D, Al Tawari A, Heberle L. The microcephaly-capillary malformation syndrome in two brothers with novel clinical features. Pediatr Neurol. 2014;51:560-5 pubmed publisher
    ..This report presents the longest follow-up of patients with microcephaly-capillary syndrome so far reported and emphasize the syndrome's phenotype variability. ..
  13. Shrestha R, Ronau J, Davies C, Guenette R, Strieter E, PAUL L, et al. Insights into the mechanism of deubiquitination by JAMM deubiquitinases from cocrystal structures of the enzyme with the substrate and product. Biochemistry. 2014;53:3199-217 pubmed publisher
    b>AMSH, a conserved zinc metallo deubiquitinase, controls downregulation and degradation of cell-surface receptors mediated by the endosomal sorting complexes required for transport (ESCRT) machinery...
  14. Li J, Zhang Y, Da Silva Sil Dos Santos B, Wang F, Ma Y, Pérez C, et al. Epidithiodiketopiperazines Inhibit Protein Degradation by Targeting Proteasome Deubiquitinase Rpn11. Cell Chem Biol. 2018;25:1350-1358.e9 pubmed publisher
    ..ETPs also inhibited other JAMM (JAB1/MPN/Mov34 metalloenzyme) proteases such as Csn5 and AMSH. An improved ETP with fewer non-specific effects, SOP11, stabilized a subset of proteasome substrates in cells, ..
  15. Singhvi A, Liu B, Friedman C, Fong J, Lu Y, Huang X, et al. A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride Inhibition of an rGC. Cell. 2016;165:936-48 pubmed publisher
    ..The C. elegans AMsh glial cell ensheathes the NREs of 12 neurons, including the thermosensory neuron AFD...
  16. Kalinowska K, Nagel M, Goodman K, Cuyas L, Anzenberger F, Alkofer A, et al. Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3. Proc Natl Acad Sci U S A. 2015;112:E5543-51 pubmed publisher
    ..Associated molecule with the SH3 domain of signal transduction adaptor molecule (STAM) (AMSH) is a conserved metalloprotease DUB in eukaryotes...
  17. Zhu W, Liu Y, Ling B. Quantum Mechanics and Molecular Mechanics Study of the Catalytic Mechanism of Human AMSH-LP Domain Deubiquitinating Enzymes. Biochemistry. 2015;54:5225-34 pubmed publisher
    ..The human AMSH-LP DUB domain specifically cleaves the isopeptide bonds in the Lys63-linked polyubiquitin chains...
  18. Naseer M, Sogaty S, Rasool M, Chaudhary A, Abutalib Y, Walker S, et al. Microcephaly-capillary malformation syndrome: Brothers with a homozygous STAMBP mutation, uncovered by exome sequencing. Am J Med Genet A. 2016;170:3018-3022 pubmed publisher
    ..We compared the findings in the present brothers with those of earlier reported patients. © 2016 Wiley Periodicals, Inc. ..
  19. Spiegler S, Kirchmaier B, Rath M, Korenke G, Tetzlaff F, van de Vorst M, et al. FAM222B Is Not a Likely Novel Candidate Gene for Cerebral Cavernous Malformations. Mol Syndromol. 2016;7:144-52 pubmed publisher
    ..These observations led to the assumption that aberrant FAM222B is not involved in the formation of CCMs. ..
  20. Zhang L, Zhou H, Peng Q, Jiang W, Qiao W, Wang G. Fatty acid binding protein 3 is associated with skeletal muscle strength in polymyositis and dermatomyositis. Int J Rheum Dis. 2017;20:252-260 pubmed publisher
    ..included 24 subjects with PM/DM, 12 subjects with asymptomatic or minimally symptomatic hyper-creatine kinase-emia (AMSH), and 10 healthy control subjects. Muscle strength was measured by using the Manual Muscle Test (MMT8)...
  21. Vastrad B, Vastrad C, Tengli A, Iliger S. Identification of differentially expressed genes regulated by molecular signature in breast cancer-associated fibroblasts by bioinformatics analysis. Arch Gynecol Obstet. 2018;297:161-183 pubmed publisher
  22. Takeda A, Oberoi Khanuja T, Glatz G, Schulenburg K, Scholz R, Carpy A, et al. Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1. EMBO J. 2014;33:1784-801 pubmed publisher
    ..Our results reveal a novel, obligatory role for IAPs and ubiquitination in the physical and functional disassembly of ERK5-MAPK module and human muscle cell differentiation. ..
  23. Row P, Prior I, McCullough J, Clague M, Urbé S. The ubiquitin isopeptidase UBPY regulates endosomal ubiquitin dynamics and is essential for receptor down-regulation. J Biol Chem. 2006;281:12618-24 pubmed
    ..The cellular functions of UBPY are complex but clearly distinct from those of the Lys-63-ubiquitin-specific protease, AMSH, with which it shares a binding site on the SH3 domain of STAM.
  24. Saha S, Caviness G, Yi G, Raymond E, Mbow M, Kao C. E3 Ubiquitin Ligase RNF125 Activates Interleukin-36 Receptor Signaling and Contributes to Its Turnover. J Innate Immun. 2018;10:56-69 pubmed publisher
    ..These results demonstrate that RNF125 has multiple regulatory roles in the signaling, trafficking, and turnover of the IL-36R. ..
  25. Harkiolaki M, Lewitzky M, Gilbert R, Jones E, Bourette R, Mouchiroud G, et al. Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76. EMBO J. 2003;22:2571-82 pubmed
    ..Interestingly, the SH3C displays ion-dependent dimerization in the crystal and in solution, suggesting a novel mechanism for the regulation of SH3 domain functions. ..
  26. Baiady N, Padala P, Mashahreh B, Cohen Kfir E, Todd E, Du Pont K, et al. The Vps27/Hrs/STAM (VHS) Domain of the Signal-transducing Adaptor Molecule (STAM) Directs Associated Molecule with the SH3 Domain of STAM (AMSH) Specificity to Longer Ubiquitin Chains and Dictates the Position of Cleavage. J Biol Chem. 2016;291:2033-42 pubmed publisher
    The deubiquitinating enzyme associated molecule with the SH3 domain of STAM (AMSH) is crucial for the removal of ubiquitin molecules during receptor-mediated endocytosis and lysosomal receptor sorting...
  27. Kikuchi K, Ishii N, Asao H, Sugamura K. Identification of AMSH-LP containing a Jab1/MPN domain metalloenzyme motif. Biochem Biophys Res Commun. 2003;306:637-43 pubmed
    We have isolated a cDNA clone encoding a new AMSH (associated molecule with the SH3 domain of STAM) family protein, termed AMSH-like protein (AMSH-LP)...
  28. Nakamura M, Tanaka N, Kitamura N, Komada M. Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes. Genes Cells. 2006;11:593-606 pubmed
    ..b>AMSH is a deubiquitinating enzyme that interacts with STAM and is implicated in the down-regulation of epidermal growth ..
  29. Sato Y, Yoshikawa A, Yamagata A, Mimura H, Yamashita M, Ookata K, et al. Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains. Nature. 2008;455:358-62 pubmed publisher
    ..The Zn(2+)-dependent DUBs AMSH and AMSH-LP regulate receptor trafficking by specifically cleaving Lys 63-linked polyubiquitin chains from ..
  30. Endo K, Takeshita T, Kasai H, Sasaki Y, Tanaka N, Asao H, et al. STAM2, a new member of the STAM family, binding to the Janus kinases. FEBS Lett. 2000;477:55-61 pubmed
  31. Nakasone M, Livnat Levanon N, Glickman M, Cohen R, Fushman D. Mixed-linkage ubiquitin chains send mixed messages. Structure. 2013;21:727-40 pubmed publisher
    ..Finally, we propose a new, comprehensive notation for Ub and Ub-like polymers. ..
  32. Bueno A, Shrestha R, Ronau J, Babar A, Sheedlo M, Fuchs J, et al. Dynamics of an Active-Site Flap Contributes to Catalysis in a JAMM Family Metallo Deubiquitinase. Biochemistry. 2015;54:6038-51 pubmed publisher
    The endosome-associated deubiquitinase (DUB) AMSH is a member of the JAMM family of zinc-dependent metallo isopeptidases with high selectivity for Lys63-linked polyubiquitin chains, which play a key role in endosomal-lysosomal sorting of ..
  33. Michel M, Elliott P, Swatek K, Simíček M, Pruneda J, Wagstaff J, et al. Assembly and specific recognition of k29- and k33-linked polyubiquitin. Mol Cell. 2015;58:95-109 pubmed publisher
    ..Our work uncovers linkage-specific components in the Ub system for atypical K29- and K33-linked Ub chains, providing tools to further understand these unstudied posttranslational modifications. ..
  34. Faqeih E, Bastaki L, Rosti R, Spencer E, Zada A, Saleh M, et al. Novel STAMBP mutation and additional findings in an Arabic family. Am J Med Genet A. 2015;167A:805-9 pubmed publisher
  35. Davies C, PAUL L, Das C. Mechanism of recruitment and activation of the endosome-associated deubiquitinase AMSH. Biochemistry. 2013;52:7818-29 pubmed publisher
    b>AMSH, a deubiquitinating enzyme (DUB) with exquisite specificity for Lys63-linked polyubiquitin chains, is an endosome-associated DUB that regulates sorting of activated cell-surface signaling receptors to the lysosome, a process mediated ..
  36. McCullough J, Clague M, Urbé S. AMSH is an endosome-associated ubiquitin isopeptidase. J Cell Biol. 2004;166:487-92 pubmed
    ..We have looked for similar activity in associated molecule with the SH3 domain of STAM (AMSH), a JAMM domain-containing protein that associates with the SH3-domain of STAM, a ..
  37. McDonell L, Mirzaa G, Alcantara D, Schwartzentruber J, Carter M, Lee L, et al. Mutations in STAMBP, encoding a deubiquitinating enzyme, cause microcephaly-capillary malformation syndrome. Nat Genet. 2013;45:556-62 pubmed publisher
    ..the deubiquitinating (DUB) isopeptidase STAMBP (STAM-binding protein, also known as AMSH, associated molecule with the SH3 domain of STAM) that has a key role in cell surface receptor-mediated endocytosis and sorting...
  38. Fernández Majada V, Welz P, Ermolaeva M, Schell M, Adam A, Dietlein F, et al. The tumour suppressor CYLD regulates the p53 DNA damage response. Nat Commun. 2016;7:12508 pubmed publisher
    ..Collectively, our results identify CYLD as a deubiquitinase facilitating DNA damage-induced p53 activation and suggest that regulation of p53 responses to genotoxic stress contributes to the tumour suppressor function of CYLD. ..
  39. Wang P, Yu P, Gao P, Shi T, Ma D. Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site. BMC Genomics. 2009;10:518 pubmed publisher
    ..Our data provide a genome-wide resource for identification of novel human transcript variants with intronic polyadenylation sites, and offer a new view into the mystery of the human transcriptome. ..
  40. Bränn E, Papadopoulos F, Fransson E, White R, Edvinsson A, Hellgren C, et al. Inflammatory markers in late pregnancy in association with postpartum depression-A nested case-control study. Psychoneuroendocrinology. 2017;79:146-159 pubmed publisher
    ..The difference remained statistically significant for STAM-BP (or otherwise AMSH), AXIN-1, ADA, ST1A1 and IL-10, after Bonferroni correction...
  41. Asada H, Ishii N, Sasaki Y, Endo K, Kasai H, Tanaka N, et al. Grf40, A novel Grb2 family member, is involved in T cell signaling through interaction with SLP-76 and LAT. J Exp Med. 1999;189:1383-90 pubmed
    ..Our data suggest that Grf40 is an adaptor molecule involved in TCR-mediated signaling through a more efficient interaction than Grb2 with SLP-76 and LAT. ..
  42. Kliza K, Taumer C, Pinzuti I, Franz Wachtel M, Kunzelmann S, Stieglitz B, et al. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry. Nat Methods. 2017;14:504-512 pubmed publisher
    ..We demonstrated that linear polyubiquitination of the novel LUBAC substrate TRAF6 is essential for NF?B signaling. ..
  43. Row P, Liu H, Hayes S, Welchman R, Charalabous P, Hofmann K, et al. The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degradation. J Biol Chem. 2007;282:30929-37 pubmed
    ..In common with other MIT-containing proteins such as AMSH and VPS4, UBPY can interact with CHMP proteins, which are known to regulate endosomal sorting of ubiquitinated ..
  44. Herrera Vigenor F, Hernández García R, Valadez Sánchez M, Vazquez Prado J, Reyes Cruz G. AMSH regulates calcium-sensing receptor signaling through direct interactions. Biochem Biophys Res Commun. 2006;347:924-30 pubmed
    ..We identified AMSH, an ubiquitin isopeptidase, as a CaR-interacting partner...
  45. Fritsch J, Stephan M, Tchikov V, Winoto Morbach S, Gubkina S, Kabelitz D, et al. Cell fate decisions regulated by K63 ubiquitination of tumor necrosis factor receptor 1. Mol Cell Biol. 2014;34:3214-28 pubmed publisher
    ..Thus, K63 ubiquitination of TNF-R1 can be sensed as a new level of regulation of TNF-R1 signaling at the earliest stage after ligand binding. ..
  46. Ribeiro Rodrigues T, Catarino S, Marques C, Ferreira J, Martins Marques T, Pereira P, et al. AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions. FASEB J. 2014;28:4629-41 pubmed publisher
    ..Here we report that the DUB-associated molecule with the SH3 domain of STAM (AMSH) interacts with Cx43 and mediates its deubiquitination...
  47. Ibarrola N, Kratchmarova I, Nakajima D, Schiemann W, Moustakas A, Pandey A, et al. Cloning of a novel signaling molecule, AMSH-2, that potentiates transforming growth factor beta signaling. BMC Cell Biol. 2004;5:2 pubmed
    ..a novel molecule from the HUGE database that is related to a previously known molecule, AMSH (associated molecule with the SH3 domain of STAM), an adapter shown to be involved in BMP signaling...
  48. Mukai A, Mizuno E, Kobayashi K, Matsumoto M, Nakayama K, Kitamura N, et al. Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis. J Cell Sci. 2008;121:1325-33 pubmed publisher
    ..Two deubiquitylating enzymes UBPY and AMSH, which act on endosomes in interphase, were also recruited to the cleavage region...
  49. Hologne M, Cantrelle F, Rivière G, Guillière F, Trivelli X, Walker O. NMR Reveals the Interplay among the AMSH SH3 Binding Motif, STAM2, and Lys63-Linked Diubiquitin. J Mol Biol. 2016;428:4544-4558 pubmed publisher
    b>AMSH [associated molecule with a Src homology 3 domain of signal transducing adaptor molecule (STAM)] is one of the deubiquitinating enzymes associated in the regulation of endocytic cargo trafficking...
  50. Komander D, Reyes Turcu F, Licchesi J, Odenwaelder P, Wilkinson K, Barford D. Molecular discrimination of structurally equivalent Lys 63-linked and linear polyubiquitin chains. EMBO Rep. 2009;10:466-73 pubmed publisher
    ..Our results highlight remarkable specificity determinants within the ubiquitin system. ..
  51. Li H, Seth A. An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein. Oncogene. 2004;23:1801-8 pubmed
    ..Among 18 in-frame positive clones, three were found to be ZBRK1, Eps15 and AMSH (associated molecule with the SH3 domain of STAM)...
  52. Castañeda C, Dixon E, Walker O, Chaturvedi A, Nakasone M, Curtis J, et al. Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. Structure. 2016;24:423-36 pubmed publisher
    ..Binding studies and mutagenesis confirmed this prediction, further highlighting structural/recognition versatility of polyubiquitins and the potential power of determining function from elucidation of conformational ensembles. ..
  53. Solomons J, Sabin C, Poudevigne E, Usami Y, Hulsik D, Macheboeuf P, et al. Structural basis for ESCRT-III CHMP3 recruitment of AMSH. Structure. 2011;19:1149-59 pubmed publisher
    ..We present the crystal structure of an N-terminal fragment of the deubiquitinating enzyme AMSH (AMSHΔC) in complex with the C-terminal region of ESCRT-III CHMP3 (CHMP3ΔN)...
  54. Hasdemir B, Murphy J, Cottrell G, Bunnett N. Endosomal deubiquitinating enzymes control ubiquitination and down-regulation of protease-activated receptor 2. J Biol Chem. 2009;284:28453-66 pubmed publisher
    ..deubiquitination occurs between early endosomes and lysosomes and involves the endosomal deubiquitinating proteases AMSH and UBPY...
  55. Zamborlini A, Usami Y, Radoshitzky S, Popova E, Palu G, Gottlinger H. Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Proc Natl Acad Sci U S A. 2006;103:19140-5 pubmed
    ..The acidic half of CHMP3 also interacts with the endosome-associated ubiquitin isopeptidase AMSH, and the coexpression of AMSH or its CHMP3-binding domain converts wild-type CHMP3 into a potent inhibitor of HIV-1 ..
  56. Berry D, Nash P, Liu S, Pawson T, McGlade C. A high-affinity Arg-X-X-Lys SH3 binding motif confers specificity for the interaction between Gads and SLP-76 in T cell signaling. Curr Biol. 2002;12:1336-41 pubmed
    ..These results provide a molecular explanation for the specific role of Gads in T cell receptor signaling, and identify a discrete subclass of SH3 domains whose binding is dependent on a core R-X-X-K motif. ..
  57. Lata S, Roessle M, Solomons J, Jamin M, Gottlinger H, Svergun D, et al. Structural basis for autoinhibition of ESCRT-III CHMP3. J Mol Biol. 2008;378:818-27 pubmed publisher
    ..Both the closed and open conformations of CHMP3 interact with AMSH with high affinity...
  58. Bednash J, Weathington N, Londino J, Rojas M, Gulick D, Fort R, et al. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Nat Commun. 2017;8:15203 pubmed publisher
    ..These findings describe a unique pathway of inflammasome regulation with the identification of STAMBP as a potential therapeutic target to reduce pro-inflammatory stress. ..
  59. Volik S, Raphael B, Huang G, Stratton M, Bignel G, Murnane J, et al. Decoding the fine-scale structure of a breast cancer genome and transcriptome. Genome Res. 2006;16:394-404 pubmed
    ..Given these properties, ESP could play an important role in a tumor genome project. ..
  60. Trang V, Rodgers M, Boyle K, Hoskins A, Strieter E. Chemoenzymatic synthesis of bifunctional polyubiquitin substrates for monitoring ubiquitin chain remodeling. Chembiochem. 2014;15:1563-8 pubmed publisher
    ..In summary, this work provides a powerful new tool for elucidating the mechanisms of DUBs and other ubiquitin processing enzymes. ..
  61. Bremm A, Freund S, Komander D. Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne. Nat Struct Mol Biol. 2010;17:939-47 pubmed publisher
    ..Our data highlight the intrinsic specificity of the ubiquitin system that extends to Lys11-linked chains and emphasize that differentially linked polyubiquitin chains must be regarded as independent post-translational modifications. ..
  62. Meijer I, van Rotterdam W, van Zoelen E, van Leeuwen J. Recycling of EGFR and ErbB2 is associated with impaired Hrs tyrosine phosphorylation and decreased deubiquitination by AMSH. Cell Signal. 2012;24:1981-8 pubmed publisher
    ..EGFR and the EGFR-ErbB2 chimera is associated with tyrosine phosphorylation of the ESCRT-0 complex subunit Hrs and AMSH-mediated deubiquitination...