alpha tocopherol transfer protein

Summary

Gene Symbol: alpha tocopherol transfer protein
Description: tocopherol (alpha) transfer protein
Alias: ATTP, AVED, TTP1, alphaTTP, alpha-TTP, alpha-tocopherol transfer protein, tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin E deficiency)
Species: human

Top Publications

  1. ncbi Mycobacteriophage D29 contains an integration system similar to that of the temperate mycobacteriophage L5
    G Ribeiro
    Laboratory of Molecular Virology, Gulbenkian Institute of Science, Oeiras, Portugal
    Microbiology 143:2701-8. 1997
  2. pmc A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector
    Shigeyuki Yamaguchi
    Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Yonago, Japan
    PLoS ONE 6:e17267. 2011
  3. ncbi Intramolecular integration assay validates integrase phi C31 and R4 potential in a variety of insect cells
    Jakkrawarn Chompoosri
    Department of Biological Sciences, Eck Institute of Global Health, University of Notre Dame, Notre Dame, IN 46556, USA
    Southeast Asian J Trop Med Public Health 40:1235-53. 2009
  4. pmc Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555 Japan
    Malar J 9:101. 2010
  5. pmc Alpha-Tocopherol Transfer Protein (alpha-TTP): Insights from Alpha-Tocopherol Transfer Protein Knockout Mice
    Yunsook Lim
    Department of Food and Nutrition, Kyung Hee University, Seoul 130 701, Korea
    Nutr Res Pract 1:247-53. 2007
  6. doi Epidemiological, clinical, paraclinical and molecular study of a cohort of 102 patients affected with autosomal recessive progressive cerebellar ataxia from Alsace, Eastern France: implications for clinical management
    M Anheim
    Departement de Neurologie, Hopital Civil, Centre Hospitalier Universitaire de Strasbourg, 1, Place de l Hopital, 67000, Strasbourg, France
    Neurogenetics 11:1-12. 2010
  7. ncbi Vitamin E: action, metabolism and perspectives
    E Herrera
    Facultad de Ciencias Experimentales y Técnicas, Universidad San Pablo CEU, Madrid, Spain
    J Physiol Biochem 57:43-56. 2001
  8. ncbi Genetic analysis of early onset cerebellar ataxia with retained tendon reflexes in four Tunisian families
    N Marzouki
    Labratoire de Génétique Moléculaire, Université Mohamed Ben Abdellah, Faculte des Sciences, Fes, Morocco
    Clin Genet 59:257-62. 2001
  9. pmc Depletion of vitamin E increases amyloid beta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer disease
    Yoichiro Nishida
    Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519 Japan
    J Biol Chem 284:33400-8. 2009
  10. ncbi Vitamin E trafficking
    Maret G Traber
    Linus Pauling Institute, Oregon State University, Corvallis, OR 97331 6512, USA
    Ann N Y Acad Sci 1031:1-12. 2004

Research Grants

  1. PATHWAYS OF LAMBDA SITE SPECIFIC RECOMBINATION
    Anca Segall; Fiscal Year: 1999
  2. Integration and Excision by Serine Intergrases
    Graham F Hatfull; Fiscal Year: 2010
  3. X-ray Structural Studies of Retinal Dystrophies
    Kwan Hong Min; Fiscal Year: 2007
  4. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2009
  5. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2009
  6. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2010
  7. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2007
  8. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2007
  9. Site-specific transgenesis in zebrafish
    JAMES LISTER; Fiscal Year: 2007
  10. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2002

Detail Information

Publications160 found, 100 shown here

  1. ncbi Mycobacteriophage D29 contains an integration system similar to that of the temperate mycobacteriophage L5
    G Ribeiro
    Laboratory of Molecular Virology, Gulbenkian Institute of Science, Oeiras, Portugal
    Microbiology 143:2701-8. 1997
    ..excisase gene and the integrase gene had a lower than average G+C content and showed the presence of the same attP core sequence as mycobacteriophage L5...
  2. pmc A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector
    Shigeyuki Yamaguchi
    Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Yonago, Japan
    PLoS ONE 6:e17267. 2011
    ..We designed a human artificial chromosome (HAC) vector containing five recombination sites (ΦC31 attP, R4 attP, TP901-1 attP, Bxb1 attP and FRT; multi-integrase HAC vector) and de novo mammalian codon-optimized ..
  3. ncbi Intramolecular integration assay validates integrase phi C31 and R4 potential in a variety of insect cells
    Jakkrawarn Chompoosri
    Department of Biological Sciences, Eck Institute of Global Health, University of Notre Dame, Notre Dame, IN 46556, USA
    Southeast Asian J Trop Med Public Health 40:1235-53. 2009
    ..We have analyzed the ability of these integrases to mediate intramolecular integration between their attB and attP sites in 7 important insect cell lines as a means of predicting their relative mobility in the corresponding insect ..
  4. pmc Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555 Japan
    Malar J 9:101. 2010
    ..However, mechanisms of this Plasmodium inhibition, in addition to means by which to exploit this finding as a therapeutic strategy, remain unclear...
  5. pmc Alpha-Tocopherol Transfer Protein (alpha-TTP): Insights from Alpha-Tocopherol Transfer Protein Knockout Mice
    Yunsook Lim
    Department of Food and Nutrition, Kyung Hee University, Seoul 130 701, Korea
    Nutr Res Pract 1:247-53. 2007
    ..Furthermore, alpha-TTP(-/-) mice appear to be useful to elucidate functions of alpha-TTP beyond its well recognized functions of transferring alpha-T from liver to plasma lipoprotein fractions...
  6. doi Epidemiological, clinical, paraclinical and molecular study of a cohort of 102 patients affected with autosomal recessive progressive cerebellar ataxia from Alsace, Eastern France: implications for clinical management
    M Anheim
    Departement de Neurologie, Hopital Civil, Centre Hospitalier Universitaire de Strasbourg, 1, Place de l Hopital, 67000, Strasbourg, France
    Neurogenetics 11:1-12. 2010
    ..with autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), one with ataxia with vitamin E deficiency (AVED) and one with autosomal recessive cerebellar ataxia type 2 (ARCA2)...
  7. ncbi Vitamin E: action, metabolism and perspectives
    E Herrera
    Facultad de Ciencias Experimentales y Técnicas, Universidad San Pablo CEU, Madrid, Spain
    J Physiol Biochem 57:43-56. 2001
    ..The most intriguing is its interaction with other antioxidants that may explain how foods containing small amounts of vitamin E provide greater benefits than larger doses of vitamin E alone...
  8. ncbi Genetic analysis of early onset cerebellar ataxia with retained tendon reflexes in four Tunisian families
    N Marzouki
    Labratoire de Génétique Moléculaire, Université Mohamed Ben Abdellah, Faculte des Sciences, Fes, Morocco
    Clin Genet 59:257-62. 2001
    ..that three of the four families studied were not linked to Friedreich's ataxia (FA), vitamin E deficiency ataxia (AVED), and autosomal dominant cerebellar ataxia (ADCA) loci...
  9. pmc Depletion of vitamin E increases amyloid beta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer disease
    Yoichiro Nishida
    Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519 Japan
    J Biol Chem 284:33400-8. 2009
    ..These results suggest that lipid peroxidation due to depletion of alpha-tocopherol impairs Abeta clearances from the brain and from the blood, possibly causing increased Abeta accumulation in Ttpa(-/-)APPsw mouse brain and plasma...
  10. ncbi Vitamin E trafficking
    Maret G Traber
    Linus Pauling Institute, Oregon State University, Corvallis, OR 97331 6512, USA
    Ann N Y Acad Sci 1031:1-12. 2004
    ..Clearly, critical information is lacking in our understanding of the mechanism by which alpha-TTP facilitates the preferential enrichment of VLDLs with alpha-tocopherol...
  11. doi ATP-binding cassette transporter A1 is involved in hepatic alpha-tocopherol secretion
    Mototada Shichiri
    Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    J Nutr Biochem 21:451-6. 2010
    ..In a mouse in vivo experiment, addition of 1% probucol to the diet decreased plasma alpha-tocopherol concentrations. These results strongly suggest that ABCA1 is substantially involved in hepatic alpha-tocopherol secretion...
  12. ncbi Molecular mechanisms of vitamin E transport
    Achim Stocker
    Institute of Microbiology, Swiss Federal Institute of Technology Zurich, Schmelzbergstr 7, 8092 Zurich, Switzerland
    Ann N Y Acad Sci 1031:44-59. 2004
    ....
  13. pmc Integration into the phage attachment site, attB, impairs multicellular differentiation in Stigmatella aurantiaca
    Susanne Muller
    Georgia Institute of Technology, School of Biology, 310 Ferst Drive, Atlanta, Georgia 30332 0230, USA
    J Bacteriol 188:1701-9. 2006
    ..However, plasmids containing Mx8 fragments encoding the integrase and attP are able to integrate at the attB locus in the S. aurantiaca genome by site-specific recombination...
  14. pmc Genomic characterization of the filamentous integrative bacteriophages {phi}RSS1 and {phi}RSM1, which infect Ralstonia solanacearum
    Takeru Kawasaki
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1 3 1 Kagamiyama, Higashi Hiroshima 739 8530, Japan
    J Bacteriol 189:5792-802. 2007
    ..Interestingly, ORF14, located next to the attP site on the phiRSM1 genome, showed high amino acid sequence homology with bacterial DNA recombinases and resolvases,..
  15. ncbi [Negative cortisone attitude in patients with bronchial asthma]
    W Woller
    Klinisches Institut und Klinik für Psychosomatische Medizin und Psychotherapie, Heinrich Heine Universitat Dusseldorf
    Pneumologie 46:326-9. 1992
    ..with bronchial asthma before and after participation in the Düsseldorf Asthma treatment and teaching programme (ATTP) and one year later...
  16. doi A novel delins mutation in the alpha-TTP gene in a family segregating ataxia with isolated vitamin E deficiency
    Miguel Fernandez-Burriel
    Research Unit Clinical Chemistry Laboratory, Hospital de Mérida, Merida, Badajoz, Spain
    Pediatr Res 64:262-4. 2008
    ..These findings suggest that other genes may play a role in producing this atypical biochemical profile...
  17. ncbi Gene expression profile of oxidant stress and neurodegeneration in transgenic mice deficient in alpha-tocopherol transfer protein
    Kishorchandra Gohil
    Center for Comparative Respiratory and Medicine, Department of Internal Medicine, University of California, Davis, CA 95616, USA
    Free Radic Biol Med 35:1343-54. 2003
    ..The repression of specific genes that determine synaptic plasticity and neuronal development may account for suppressed electrophysiological activities of cortex and impaired behavior in Ttpa(-/-) mice...
  18. ncbi pH-dependent translocation of alpha-tocopherol transfer protein (alpha-TTP) between hepatic cytosol and late endosomes
    Masakuni Horiguchi
    Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan
    Genes Cells 8:789-800. 2003
    ..To explore the mechanism of alpha-TTP mediated alpha-tocopherol secretion, we investigated drugs which may affect this secretion...
  19. doi SXT-related integrating conjugative element and IncC plasmids in Vibrio cholerae O1 strains in Eastern Africa
    N Pugliese
    Dipartimento di Genetica e Microbiologia, Universita di Bari, Bari, Italy
    J Antimicrob Chemother 63:438-42. 2009
    ..The objective of this study was to investigate the extent of resistance patterns and associated mobile genetic elements in epidemic V. cholerae O1 El Tor strains isolated from Eastern Africa in the late 1990s...
  20. pmc Mice lacking alpha-tocopherol transfer protein gene have severe alpha-tocopherol deficiency in multiple regions of the central nervous system
    Kishorchandra Gohil
    Department of Internal Medicine, Genome and Biomedical Sciences Facility, University of California, Davis, CA 95616, USA
    Brain Res 1201:167-76. 2008
    ..Interestingly, functional abnormality (ataxia) develops only very late in alpha-TTP-KO mice in spite of the severe alpha-tocopherol deficiency in the brain starting at an early age...
  21. ncbi Sequence analysis of a unique temperature phage: mycoplasma virus L2
    J Maniloff
    Department of Microbiology and Immunology, University of Rochester, NY 14642
    Gene 141:1-8. 1994
    ..The putative attP integration site has been mapped to an intergenic region, 280-bp downstream from ORF 5...
  22. pmc Delayed-onset ataxia in mice lacking alpha -tocopherol transfer protein: model for neuronal degeneration caused by chronic oxidative stress
    T Yokota
    Department of Neurology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Proc Natl Acad Sci U S A 98:15185-90. 2001
    ..Mutations of the alpha-TTP gene are linked to ataxia with isolated vitamin E deficiency (AVED)...
  23. ncbi Localization of alpha-tocopherol transfer protein in the brains of patients with ataxia with vitamin E deficiency and other oxidative stress related neurodegenerative disorders
    R P Copp
    Department of Medicine, New York University Medical Center, 550 First Avenue, New York, NY 10016, USA
    Brain Res 822:80-7. 1999
    ..are associated with a neurological syndrome of spinocerebellar ataxia, called ataxia with vitamin E deficiency (AVED). Earlier studies suggested that alpha-TTP is found only in the liver...
  24. ncbi Acceleration of age-related changes in the retina in alpha-tocopherol transfer protein null mice fed a Vitamin E-deficient diet
    Masaki Tanito
    Department of Ophthalmology, Shimane University School of Medicine, Shimane, Japan
    Invest Ophthalmol Vis Sci 48:396-404. 2007
    ..To assess the role of vitamin E (VE) in age-related changes in the retinal tissues by using a mouse model of severe VE deficiency...
  25. ncbi Tocopherol transfer protein deficiency modifies nuclear receptor transcriptional networks in lungs: modulation by cigarette smoke in vivo
    K Gohil
    Pulmonary and Critical Care Medicine, Genome and Biomedical Sciences Facility, 451 East Health Sciences Drive, University of California, Davis, CA 95616, USA
    Mol Aspects Med 28:453-80. 2007
    ..this study we have used mice with severe AT deficiency inflicted at birth by the deletion of AT transfer protein (ATTP) which is abundantly expressed in the liver and regulates systemic concentrations of AT...
  26. doi Ataxia with vitamin E deficiency: update of molecular diagnosis
    I Di Donato
    Dipartimento di Scienze Neurologiche, Neurochirurgiche e del Comportamento, Universita degli Studi di Siena, Viale Bracci, 53100, Siena, Italy
    Neurol Sci 31:511-5. 2010
    Ataxia with vitamin E deficiency (AVED) is a rare autosomal recessive neurodegenerative disease, due to mutations in TTPA gene (Arita et al. in Biochem J 306(Pt. 2):437-443, 1995; Hentati et al...
  27. ncbi Clinical comparison between AVED patients with 744 del A mutation and Friedreich ataxia with GAA expansion in 15 Moroccan families
    Ali Benomar
    Laboratoire de Neurogénétique, Service de Neurologie, Hopital des specialites, BP 6220 Rabat Instituts, Rabat, Morocco
    J Neurol Sci 198:25-9. 2002
    ..in the alpha-tocopherol transfer protein (alpha-TTP) gene, characteristic of ataxia with vitamin E deficiency (AVED). The other eight families (16 patients) had GAA expansions in the first intron of the frataxin gene...
  28. ncbi Galactosamine-induced acute liver injury in rats reduces hepatic alpha-tocopherol transfer protein production
    Asako Takenaka
    Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Yamagata, Japan
    J Nutr Sci Vitaminol (Tokyo) 53:366-71. 2007
    ..Serum alpha-tocopherol concentration was not directly affected by the acute change in hepatic alpha-TTP level, suggesting that the chronic changes in alpha-TTP activity would be necessary to regulate serum alpha-tocopherol concentration...
  29. ncbi Selective accumulation of alpha-tocopherol in Drosophila is associated with cytochrome P450 tocopherol-omega-hydroxylase activity but not alpha-tocopherol transfer protein
    Robert S Parker
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Biochem Biophys Res Commun 338:1537-41. 2005
    ..The selective pressure favoring this trait and its remarkable conservation from insects to humans requires elucidation...
  30. ncbi Dietary zinc restriction in rats alters antioxidant status and increases plasma F2 isoprostanes
    Richard S Bruno
    Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA
    J Nutr Biochem 18:509-18. 2007
    ..Collectively, zinc deficiency increased oxidative stress, which may be partially explained by increased CYP activity and reductions in hepatic alpha-tocopherol and gamma-tocopherol and in plasma uric acid...
  31. pmc Isolation of new Stenotrophomonas bacteriophages and genomic characterization of temperate phage S1
    Pilar Garcia
    Area de Microbiologia, Facultad de Medicina, Universidad de Oviedo, Spain
    Appl Environ Microbiol 74:7552-60. 2008
    ..The attP site of S1 may be located within a gene that presents over 75% homology to a Stenotrophomonas chromosomal ..
  32. ncbi A method to maintain introduced DNA sequences stably and safely on the bacterial chromosome: application of prophage integration and subsequent designed excision
    M Shimizu-Kadota
    Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186 8650, Japan
    J Biotechnol 89:73-9. 2001
    ..gene as a selection marker, N- and C-terminally-truncated Cm-resistance genes were inserted into both sides of the attP of phi FSW, within which the site-specific recombination took place with the attB of phi FSW on the recipient ..
  33. ncbi Control of prophage integration and excision in bacteriophage P2: nucleotide sequences of the int gene and att sites
    A Yu
    Department of Microbial Genetics, Karolinska Institutet, Stockholm, Sweden
    Gene 80:1-11. 1989
    ..P2 into the Escherichia coli host genome involves recombination between two specific attachment sites, attP and attB, one on the phage and the other on the host genome, respectively...
  34. pmc Spontaneous excision of the Salmonella enterica serovar Enteritidis-specific defective prophage-like element phiSE14
    Carlos A Santiviago
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, P O Box 174, Correo 22, Santiago, Chile
    J Bacteriol 192:2246-54. 2010
    ..The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed...
  35. pmc Study of plastid genome stability in tobacco reveals that the loss of marker genes is more likely by gene conversion than by recombination between 34-bp loxP repeats
    Tarinee Tungsuchat-Huang
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
    Plant Physiol 153:252-9. 2010
    ..The P1 phage loxP sites are identical 34-bp direct repeats, whereas the phiC31 phage attB/attP sites are 54- and 215-bp sequences with partial homology within the 54-bp region...
  36. ncbi Tocopherol-associated protein is a ligand-dependent transcriptional activator
    J Yamauchi
    Division of Applied Food Research, National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjyuku, Japan
    Biochem Biophys Res Commun 285:295-9. 2001
    ..These results suggest that the biological function of alpha-tocopherol is not only as an antioxidant but also as a transcriptional regulator of gene expression via association with a transcription factor TAP...
  37. ncbi Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces
    F Boccard
    Laboratoire de Biologie et Génétique Moléculaire, URA CNRS D 0086, Orsay, France
    Plasmid 21:59-70. 1989
    ..A 58-bp sequence (att) present in both pSAM2 (attP) and S...
  38. ncbi Protective effect of vitamin E supplements on experimental atherosclerosis is modest and depends on preexisting vitamin E deficiency
    Cacang Suarna
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia
    Free Radic Biol Med 41:722-30. 2006
    ..These results show that vitamin E at best has a modest effect on experimental atherosclerosis in hyperlipidemic mice, and only in situations of severe vitamin E deficiency and independent of lipid oxidation in the vessel wall...
  39. ncbi Molecular genetic and expression analysis of alpha-tocopherol transfer protein mRNA in German shepherd dogs with degenerative myelopathy
    Henry Fechner
    Department of Cardiology and Pneumology, University Hospital Benjamin Franklin, Freie Universitat, Berlin, Germany
    Berl Munch Tierarztl Wochenschr 116:31-6. 2003
    ..we analyzed the expression and the nucleotide sequence of the canine alpha-tocopherol transfer protein (alpha Ttp) of German shepherd dogs with DM in order to determine whether a deficiency or a defect of the alpha Ttp could ..
  40. ncbi Liver alpha-tocopherol transfer protein and its mRNA are differentially altered by dietary vitamin E deficiency and protein insufficiency in rats
    H M Shaw
    Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 10716
    J Nutr 128:2348-54. 1998
    ..On the other hand, protein inadequacy appeared to down-regulate the expression of the alpha-TTP gene...
  41. ncbi Structure and function of alpha-tocopherol transfer protein: implications for vitamin E metabolism and AVED
    K Christopher Min
    Department of Neurology, Columbia University, New York, New York 10032, USA
    Vitam Horm 76:23-43. 2007
    ..in the protein are a cause of a progressive neurodegenerative disorder known as ataxia with vitamin E deficiency (AVED)...
  42. ncbi Complete nucleotide sequence, molecular analysis and genome structure of bacteriophage A118 of Listeria monocytogenes: implications for phage evolution
    M J Loessner
    Institut fur Mikrobiologie, FML Weihenstephan, Technische Universitat Munchen, Weihenstephaner Berg 3, 85350 Freising, Germany
    Mol Microbiol 35:324-40. 2000
    ..We have also identified the A118 attachment site attP and the corresponding attB in Listeria monocytogenes, and show that site-specific integration of the A118 prophage ..
  43. pmc Characterization of the integrase gene and attachment site for the Myxococcus xanthus bacteriophage Mx9
    Bryan Julien
    Kosan Biosciences, Inc, Hayward, California 94545, USA
    J Bacteriol 185:6325-30. 2003
    ..Mx9 integration requires a single protein, Int. Analysis of integration revealed that the phage attachment site (attP) is contained in the int gene and that upon integration, the 3' end of the int gene is altered...
  44. pmc Increased atherosclerosis in hyperlipidemic mice deficient in alpha -tocopherol transfer protein and vitamin E
    Y Terasawa
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, CA 94141 9100, USA
    Proc Natl Acad Sci U S A 97:13830-4. 2000
    ..Ttpa(-/-) mice are a genetic model of vitamin E deficiency and should be valuable for studying other diseases in which oxidative stress is thought to play a role...
  45. doi Conjugated linoleic acid causes a marked increase in liver alpha-tocopherol and liver alpha-tocopherol transfer protein in C57BL/6 J mice
    Pei Min Chao
    Institute of Nutrition and Department of Nutrition, China Medical University, Taichung, Taiwan
    Int J Vitam Nutr Res 80:65-73. 2010
    ..The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to alpha-tocopherol accumulation...
  46. ncbi Genetic vitamin E deficiency does not affect MPTP susceptibility in the mouse brain
    Yong Ri Ren
    Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan
    J Neurochem 98:1810-6. 2006
    ..Our results suggest that vitamin E does not play a major protective role in MPTP-induced nigrostriatal dopaminergic neurodegeneration in the brain...
  47. pmc Postmortem study of ataxia with retinitis pigmentosa by mutation of the alpha-tocopherol transfer protein gene
    T Yokota
    Department of Neurology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    J Neurol Neurosurg Psychiatry 68:521-5. 2000
    ..It is therefore considered that oral supplementation of vitamin E should effectively counteract the progression of ataxia...
  48. doi Severe Vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress and inflammation
    Shigeo Yamaoka
    Department of Pediatrics, Perinatal Center, Osaka Medical College, Takatsuki, Osaka, Japan
    Free Radic Res 42:602-12. 2008
    ..It was concluded that severe vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress or inflammation...
  49. ncbi Vitamin E deficiency ataxia with (744 del A) mutation on alpha-TTP gene: genetic and clinical peculiarities in Moroccan patients
    Naima Marzouki
    Molecular Genetics Laboratory, Faculty of Sciences, The Department of Neurology, University of Fez Sidi Med Benabdellah, Hospital of Rabat, Morocco
    Eur J Med Genet 48:21-8. 2005
    Ataxia with vitamin E deficiency (AVED) is an autosomal recessive disease characterized clinically by neurological symptoms with often striking resemblance to those of Friedreich's ataxia (FA)...
  50. ncbi Suicide substrates reveal properties of the homology-dependent steps during integrative recombination of bacteriophage lambda
    A B Burgin
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland 20892 4034, USA
    Curr Biol 5:1312-21. 1995
    ....
  51. pmc Ataxia caused by mutations in the alpha-tocopherol transfer protein gene
    F Usuki
    Department of Clinical Medicine, National Institute for Minamata Disease, 4058 18 Hama, Minamata 867 0008, Japan
    J Neurol Neurosurg Psychiatry 69:254-6. 2000
    ..Supplementary therapy increased her serum vitamin E concentration to the normal range with mild improvement of the deep senses...
  52. pmc The Bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages
    Shanmuga Sozhamannan
    Biological Defense Research Directorate, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, Maryland 20852, USA
    BMC Microbiol 6:34. 2006
    ..The B. anthracis genome sequence contains four putative lambdoid prophages. We undertook this study in order to understand whether the four prophages are unique to B. anthracis and whether they produce active phages...
  53. ncbi Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296
    M M Adams
    Division of Biochemistry and Molecular Biology, School of Life Sciences, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    Microbiology 147:851-60. 2001
    ..DNAs homologous to bacteriophage int and attP were located upstream of gtrA(IV), suggesting that this region of the NCTC 8296 genome may have originated from a ..
  54. ncbi Ataxia with vitamin E deficiency and severe dystonia: report of a case
    Agathe Roubertie
    Service de Neuropediatrie, Hopital Saint Eloi, 80 avenue Augustin Fliche, 34295 Montpellier Cedex 5, France
    Brain Dev 25:442-5. 2003
    ..Dystonia has rarely been described in ataxia with isolated vitamin E deficiency (AVED) patients...
  55. pmc Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase
    Louis J Nkrumah
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Methods 3:615-21. 2006
    ..This is mediated by mycobacteriophage Bxb1 integrase, which catalyzes recombination between an incoming attP and a chromosomal attB site. We developed P...
  56. ncbi Characterization of XerC- and XerD-dependent CTX phage integration in Vibrio cholerae
    Sarah M McLeod
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, MA 02111, USA
    Mol Microbiol 54:935-47. 2004
    ..cholerae chromosome. Here, we define the elements on the phage genome (attP) and bacterial chromosome (attB) required for CTXphi integration...
  57. pmc Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells
    Susanne Andreas
    Artemis Pharmaceuticals GmbH, Neurather Ring 1, 51063 Köln, Germany
    Nucleic Acids Res 30:2299-306. 2002
    The integrase of the phage PhiC31 recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host...
  58. doi TG1 integrase-based system for site-specific gene integration into bacterial genomes
    Tetsurou Muroi
    Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, 1 Nakagawara, Tokusada, Tamura machi, Koriyama, Fukushima 963 8642, Japan
    Appl Microbiol Biotechnol 97:4039-48. 2013
    Serine-type phage integrases catalyze unidirectional site-specific recombination between the attachment sites, attP and attB, in the phage and host bacterial genomes, respectively; these integrases and DNA target sites function ..
  59. pmc Repetitive, marker-free, site-specific integration as a novel tool for multiple chromosomal integration of DNA
    Kia Vest Petersen
    Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark
    Appl Environ Microbiol 79:3563-9. 2013
    ..in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB)...
  60. doi Chicken hypersensitive site-4 insulator increases human serum albumin expression in bovine mammary epithelial cells modified with phiC31 integrase
    Yan Luo
    College of Veterinary Medicine, Northwest A and F University, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling 712100, Shaanxi, People s Republic of China
    Biotechnol Lett 35:529-37. 2013
    ..A preferred pseudo attP site, which had 38 % identity with the 39 bp wild-type attP sequence, was detected in six out of 55 bMEC colonies...
  61. ncbi A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis
    Ashraf M Ahmed
    Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, 1 4 4 Kagamiyama, Higashi Hiroshima 739 8528, Japan
    FEMS Microbiol Lett 242:241-7. 2005
    ..By using PCR, we detected the antibiotic resistance genes, the integrase gene and the attP attachment site of SXT element...
  62. pmc Expression of the α-tocopherol transfer protein gene is regulated by oxidative stress and common single-nucleotide polymorphisms
    Lynn Ulatowski
    Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Free Radic Biol Med 53:2318-26. 2012
    ..These findings may explain the variable responses to vitamin E supplementation observed in human clinical trials. ..
  63. ncbi Identification of pseudo attP sites for phage phiC31 integrase in bovine genome
    Qing wen Ma
    Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, 24 1400 West Beijing Road, Shanghai 200040, PR China
    Biochem Biophys Res Commun 345:984-8. 2006
    Streptomyces phage phiC31 integrase was found to mediate site-specific integration of foreign genes at pseudo attP sites of genomes in human, mouse, rat, and Drosophila...
  64. pmc A resource for manipulating gene expression and analyzing cis-regulatory modules in the Drosophila CNS
    Laurina Manning
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
    Cell Rep 2:1002-13. 2012
    ..system expression of 5,000 GAL4 lines made using molecularly defined cis-regulatory DNA inserted into a single attP genomic location...
  65. doi Large serine recombinase domain structure and attachment site binding
    Gregory D Van Duyne
    Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
    Crit Rev Biochem Mol Biol 48:476-91. 2013
    ..The LSRs that function as bacteriophage integrases carry out integration between attachment sites in the phage (attP) and in the host (attB)...
  66. ncbi Unequal access of chromosomal regions to each other in Salmonella: probing chromosome structure with phage lambda integrase-mediated long-range rearrangements
    Nathalie Garcia-Russell
    Department of Biology and Center for Microbial Sciences, San Diego State University, 5500 Campanile Dr LS416, San Diego, CA 92182 4614, USA
    Mol Microbiol 52:329-44. 2004
    ..Recombination events were assayed by semi-quantitative polymerase chain reaction of the attP product...
  67. ncbi Expression and refolding of recombinant human alpha-tocopherol transfer protein capable of specific alpha-tocopherol binding
    Candace Panagabko
    Department of Chemistry and Centre for Biotechnology, Brock University, St Catharines, Ontario, Canada
    Protein Expr Purif 24:395-403. 2002
    ..The resulting purification scheme provides recombinant alpha-TTP in good yield and purity for investigation of both its structure and its binding affinities for different ligands including natural and synthetic tocols...
  68. pmc A phage integrase directs efficient site-specific integration in human cells
    A C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Proc Natl Acad Sci U S A 97:5995-6000. 2000
    The integrase from the Streptomyces phage phiC31 carries out efficient recombination between the attP site in the phage genome and the attB site in the host bacterial chromosome...
  69. ncbi Phage integrases: biology and applications
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    J Mol Biol 335:667-78. 2004
    ..unidirectional site-specific recombination between two DNA recognition sequences, the phage attachment site, attP, and the bacterial attachment site, attB...
  70. ncbi Direct role of the himA gene product in phage lambda integration
    H I Miller
    Nature 290:523-6. 1981
    ..is accomplished by a site-specific recombination between two unique DNA sequences (attB on the bacterial genome and attP on the phage; reviewed in refs 2, 3) and requires proteins encoded by both the bacterium and the phage...
  71. doi Establishment of an improved high-efficiency thermal asymmetric interlaced PCR for identification of genomic integration sites mediated by phiC31 integrase
    Zaiwei Zhou
    Shanghai Institute of Medical Genetics, Children s Hospital of Shanghai, Shanghai Jiao Tong University School of Medicine, 24 1400 West Beijing Road, Shanghai 200040, China
    World J Microbiol Biotechnol 28:1295-9. 2012
    ..genomic modification, as it autonomously performs efficient, unidirectional, site-specific integration into pseudo attP sites of the host genome...
  72. doi A profile of native integration sites used by φC31 integrase in the bovine genome
    Lijuan Qu
    Shanghai Institute of Medical Genetics, Children s Hospital of Shanghai, Shanghai Jiao Tong University School of Medicine, 24 1400 West Beijing Road, Shanghai 200040, China
    J Genet Genomics 39:217-24. 2012
    The Streptomyces phage φC31 integrase can efficiently target attB-bearing transgenes to endogenous pseudo attP sites within mammalian genomes...
  73. doi Interspecies competition and inhibition within the oral microbial flora: environmental factors influence the inhibition of Streptococcus mutans by Streptococcus oligofermentans
    Ying Liu
    Department of Endodontics, School of Stomatology, Tianjin Medical University, Tianjin, China
    Eur J Oral Sci 120:179-84. 2012
    ..The 4-aminoantipyine (4-ATTP) method was used to measure the yield and the initial production rate of H(2) O(2) in S. oligofermentans...
  74. doi Solution-phase synthesis of branched DNA hybrids based on dimer phosphoramidites and phenolic or nucleosidic cores
    Helmut Griesser
    Institute for Organic Chemistry, University of Stuttgart, 70569 Stuttgart, Germany
    J Org Chem 77:2703-17. 2012
    ....
  75. doi Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms)
    Richard H Baltz
    CognoGen Biotechnology Consulting, 6438 North Olney Street, Indianapolis, IN 46220, USA
    J Ind Microbiol Biotechnol 39:661-72. 2012
    ..is mediated by a large serine recombinase that catalyzes unidirectional recombination between the bacteriophage attP and chromosomal attB sites...
  76. pmc Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase
    Lin Zhang
    Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
    J Bacteriol 190:6392-7. 2008
    ..We showed that this integrase has efficient integration activity with substrate DNAs containing attB and attP sites, independent of DNA supercoiling or cofactors...
  77. pmc Role of the N-terminal domain of phiC31 integrase in attB-attP synapsis
    Paul A Rowley
    Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Bacteriol 190:6918-21. 2008
    ..We show here that substitutions in amino acid V129 in the NTD can lead to defects in synapsis and DNA cleavage, indicating that the NTD also has an important role in synapsis...
  78. doi Characterization of three human sec14p-like proteins: alpha-tocopherol transport activity and expression pattern in tissues
    Jean Marc Zingg
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    Biochimie 90:1703-15. 2008
    ..Thus, the three hTAPs may mediate lipid uptake, secretion, presentation, and sub-cellular localization in a tissue-specific manner, possibly using organelle- and enzyme-specific docking sites...
  79. doi Isolated vitamin E deficiency mimicking distal hereditary motor neuropathy in a 13-year-old boy
    Carlo Fusco
    Pediatric Neurology Unit, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy
    J Child Neurol 23:1328-30. 2008
    ..To our knowledge this is the first case of isolated deficiency of vitamin E that presents the classic neurophysiologic and clinical features of distal hereditary motor neuropathy...
  80. ncbi Complete nucleotide sequence and molecular characterization of the temperate staphylococcal bacteriophage phiPVL carrying Panton-Valentine leukocidin genes
    J Kaneko
    Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai 981 8555, Japan
    Gene 215:57-67. 1998
    ..The [lukS-PV-lukF-PV], attP, and int (integrase gene) of phiPVL were all located very close to one another within a 4...
  81. pmc Characterization of the major control region of Vibrio cholerae bacteriophage K139: immunity, exclusion, and integration
    J Nesper
    Zentrum für Infektionsforschung, Universitat Wurzburg, 97070 Wurzburg, Germany
    J Bacteriol 181:2902-13. 1999
    ..Immediately downstream of gene int, the attP site was identified. Upon analysis of the corresponding attB site within the V...
  82. pmc Identification of site-specific recombination genes int and xis of the Rhizobium temperate phage 16-3
    S Semsey
    Institute for Molecular Genetics, Agricultural Biotechnology Center, Godollo, H 2100 Hungary
    J Bacteriol 181:4185-92. 1999
    ..with high efficiency into the host chromosome by site-specific recombination through DNA sequences of attB and attP. Here we report the identification of two phage-encoded genes required for recombinations at these sites: int (..
  83. doi Immunohistochemical localization of alpha-tocopherol transfer protein and lipoperoxidation products in human first-trimester and term placenta
    Daisy E Rotzoll
    Division of Neonatology, Department of Pediatrics, University of Heidelberg, INF 150, D 69120 Heidelberg, Germany
    Eur J Obstet Gynecol Reprod Biol 140:183-91. 2008
    ....
  84. ncbi Apolipoprotein B-related gene expression and ultrastructural characteristics of lipoprotein secretion in mouse yolk sac during embryonic development
    Y Terasawa
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94110, USA
    J Lipid Res 40:1967-77. 1999
    ..secretion in mouse yolk sac during embryonic development...
  85. doi Site-specific chromosomal integration mediated by phiC31 integrase
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, Stanford, CA
    Methods Mol Biol 435:165-73. 2008
    ..enzyme normally performs precise, unidirectional recombination between two attachment or att sites called attB and attP. We have shown that an attP site preintegrated into a mammalian chromosome can serve as a target for integration of ..
  86. ncbi The alpha-tocopherol transfer protein
    D Manor
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Vitam Horm 76:45-65. 2007
    ..Heritable mutations in the ttpA gene cause ataxia with vitamin E deficiency (AVED), an autosomal recessive disorder characterized by low plasma vitamin E levels and progressive neurodegeneration...
  87. ncbi Creation of engineered human embryonic stem cell lines using phiC31 integrase
    Bhaskar Thyagarajan
    Invitrogen Corporation, Carlsbad, California 92008, USA
    Stem Cells 26:119-26. 2008
    ..The pseudo-attP profile in hESC differed from those reported earlier in differentiated cells...
  88. ncbi Insertion of bacteriophage phiFSW into the chromosome of Lactobacillus casei strain Shirota (S-1): characterization of the attachment sites and the integrase gene
    M Shimizu-Kadota
    Yakult Central Institute for Microbiological Research 1796 Yaho, Kunitachi, Tokyo, Japan
    Gene 249:127-34. 2000
    ..An integration plasmid, consisting of int, the phage attachment site (attP), and a selectable marker, inserted stably into the bacterial attachment site (attB) within the common core, as did ..
  89. pmc Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, M 334, 300 Pasteur Drive, Stanford, CA 94305 5120, United States
    J Neurosci Methods 173:299-305. 2008
    ..phiC31 integrase catalyzes the sequence-specific integration of attB-containing plasmids into pseudo attP sites in mammalian genomes, to produce long-term transgene expression...
  90. pmc Sarcolipin and ubiquitin carboxy-terminal hydrolase 1 mRNAs are over-expressed in skeletal muscles of alpha-tocopherol deficient mice
    Vihas T Vasu
    Department of Internal Medicine, University of California, Davis, 95616, USA
    Free Radic Res 43:106-16. 2009
    The transcriptome of ataxic muscles from alpha-tocopherol transfer protein deficient (ATTP-KO), 23-month old, mice was compared with that of their normal littermates...
  91. ncbi A versatile method for integration of genes and gene fusions into the lambda attachment site of Escherichia coli
    T Atlung
    Department of Microbiology, Technical University of Denmark, Lyngby
    Gene 107:11-7. 1991
    ..components: (1) a DNA integration cassette, flanked by multiple restriction enzyme sites, which contains the lambda attP site and, as a selectable marker, the Tn5 aphA gene conferring kanamycin resistance (KmR); and (2) a plasmid with ..
  92. pmc Cutting out the φC31 prophage
    W Marshall Stark
    Institute of Molecular, Cell and Systems Biology, University of Glasgow, Glasgow G12 8QQ, UK
    Mol Microbiol 80:1417-9. 2011
    ..C31 integrates its genome into the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment site in the phage DNA) and attB (the chromosomal attachment site)...
  93. ncbi The nucleotide sequence of TTP1, a gene encoding a predicted type II membrane protein
    P A Romero
    McGill Cancer Centre, McGill University, , , Canada
    Yeast 10:1111-5. 1994
    ..The segment contains a new open reading frame of 1794 bp. The product encoded by the gene, designated TTP1, is a predicted type II membrane protein of 597 amino acid residues with a short cytoplasmic NH2-terminus, a ..
  94. doi Critical amino acid residues within the φC31 integrase DNA-binding domain affect recombination activities in mammalian cells
    Raphael Liesner
    Department of Virology, Max von Pettenkofer Institute, Ludwig Maximilians University Munich, Germany
    Hum Gene Ther 21:1104-18. 2010
    ..site attB within an episomal substrate plasmid and either the bacteriophage-derived wild-type attachment site attP or pseudo-attP attachment sites (attP') present in the mammalian genome...
  95. pmc Localization and nucleotide sequences of genes mediating site-specific recombination of the SLP1 element in Streptomyces lividans
    M A Brasch
    Department of Genetics, Stanford University School of Medicine, California 94305
    J Bacteriol 175:3067-74. 1993
    ..A region of SLP1 adjacent to the previously identified site of integration, attP, was found to be sufficient to promote site-specific integration of an unrelated Streptomyces plasmid...
  96. ncbi Correlation of repressed transcription of alpha-tocopherol transfer protein with serum alpha-tocopherol during hepatocarcinogenesis
    C G Wu
    Department of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands
    Int J Cancer 71:686-90. 1997
    ..Our data suggest that repressed transcription of alpha-TTP is associated with a decrease of serum alpha-tocopherol and with hepatic carcinogenesis...
  97. ncbi Transgene excision in zebrafish using the phiC31 integrase
    James A Lister
    Department of Human and Molecular Genetics, Virginia Commonwealth University School of Medicine, Richmond, Virginia
    Genesis 48:spcone. 2010
    Single optical section of a 72 hour post-fertilization T2K-XpGbR (EF1-alpha-attP-GFP-attB-DsRed-Express) transgenic zebrafish larva obtained by laser scanning confocal microscopy...
  98. doi Characterization of an SXT variant Vibrio cholerae O1 Ogawa isolated from a patient in Trivandrum, India
    Praveen Kumar
    Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India
    FEMS Microbiol Lett 303:132-6. 2010
    ..presence of SXT and its transmissibility, associated drug resistance genes and variation in the int gene and the attP attachment site of SXT...
  99. pmc Control of directionality in bacteriophage mv4 site-specific recombination: functional analysis of the Xis factor
    Michele Coddeville
    Universite de Toulouse, UPS, Laboratoire de Microbiologie et de Génétique Moléculaires, F 31000 Toulouse, France
    J Bacteriol 192:624-35. 2010
    The integrase of the temperate bacteriophage mv4 catalyzes site-specific recombination between the phage attP site and the host attB site during Lactobacillus delbrueckii lysogenization...
  100. pmc alpha-tocopherol transfer protein stimulates the secretion of alpha-tocopherol from a cultured liver cell line through a brefeldin A-insensitive pathway
    M Arita
    Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113, Japan
    Proc Natl Acad Sci U S A 94:12437-41. 1997
    ..alpha-Tocopherol transfer protein (alphaTTP), which was recently identified as a product of the causative gene for familial isolated vitamin E deficiency, is ..
  101. pmc DNA binding and synapsis by the large C-terminal domain of phiC31 integrase
    Andrew R McEwan
    Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2BX, UK
    Nucleic Acids Res 37:4764-73. 2009
    The integrase (Int) from phage C31 acts on the phage and host-attachment sites, attP and attB, to form an integrated prophage flanked by attL and attR...

Research Grants66

  1. PATHWAYS OF LAMBDA SITE SPECIFIC RECOMBINATION
    Anca Segall; Fiscal Year: 1999
    ..These two reactions differ in two fundamental ways: they contain different DNA partners (attB and attP vs attL and attR) and they have different protein requirements (int vs int+xis)...
  2. Integration and Excision by Serine Intergrases
    Graham F Hatfull; Fiscal Year: 2010
    ..More specifically, the Bxb1 integrase acts to recombine two DNA sites - attB and attP - that are small (<50bp), different in sequence and size, and requires no other DNA or protein components...
  3. X-ray Structural Studies of Retinal Dystrophies
    Kwan Hong Min; Fiscal Year: 2007
    ..determination of three proteins that are related to retinal dystrophies: alpha-tocopherol transfer protein (ATTP), cellular retinaldehyde binding protein (CRALBP), and rhodopsin...
  4. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2009
    ..The hepatic alpha tocopherol transfer protein (TTP) is a critical regulator of vitamin E status; humans carrying mutated TTP alleles develop ..
  5. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2009
    ..The hepatic alpha tocopherol transfer protein (TTP) is a critical regulator of vitamin E status; humans carrying mutated TTP alleles develop ..
  6. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2010
    ..The hepatic alpha tocopherol transfer protein (TTP) is a critical regulator of vitamin E status;humans carrying mutated TTP alleles develop severe ..
  7. Integration and excision by serine-integrases
    Graham Hatfull; Fiscal Year: 2007
    ..However, little is known about how this class of enzymes catalyzes integrative recombination between their attP and attB sites, or how this same protein catalyzes recombination between attL and attR for excision...
  8. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2007
    ..Here, we propose to study the tocopherol transfer protein (aTTP) in order to gain insights into its biological role(s) and, in turn, into the mode of action of vitamin E...
  9. Site-specific transgenesis in zebrafish
    JAMES LISTER; Fiscal Year: 2007
    ..The phiC31 integrase promotes recombination between an attachment site in the phage (attP) and another on the bacterial chromosome (attB)...
  10. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2002
    ..by the phage-encoded integrase protein which mediates site-specific recombination between the phage attachment, attP, and the chromosomal attachment site, attB...
  11. MECHANISMS OF HEMORRHAGE AND EDEMA IN TPA STROKE THERAPY
    ENG LO; Fiscal Year: 2001
    ..Since the likelihood of reperfusion injury is related to the degree of vascular damage, this method should have predictive value by assessing cerebrovascular integrity prior to thrombolytic therapy. ..
  12. Custom integration tools for functional genomics
    MICHELE CALOS; Fiscal Year: 2003
    ..in my laboratory involving recombination mediated by phage integrases at their compact (30 - 40-bp) attB and attP recognition sites...
  13. Transposon induced disruption of most Drosophila genes
    Hugo Bellen; Fiscal Year: 2007
    ..Approximately 30,000 new single Minos insertion strains containing <->C31 attP sites will be generated...
  14. Transposon induced disruption of most Drosophila genes
    Hugo Bellen; Fiscal Year: 2007
    ..Approximately 30,000 new single Minos insertion strains containing <|>C31 attP sites will be generated...
  15. Transposon induced disruption of most Drosophila genes
    Hugo J Bellen; Fiscal Year: 2010
    ..Approximately 30,000 new single Minos insertion strains containing <->C31 attP sites will be generated...
  16. Transposon induced disruption of most Drosophila genes
    Hugo Bellen; Fiscal Year: 2009
    ..Approximately 30,000 new single Minos insertion strains containing <->C31 attP sites will be generated...
  17. METHODS FOR EVALUATING VACCINE EFFICACY
    M Halloran; Fiscal Year: 2009
    ..on infectiousness, VEI, of vaccination, and the analogous effects of antiviral agents on infection, AVES, disease, AVED, and infectiousness, AVEI...
  18. METHODS FOR EVALUATING VACCINE EFFICACY
    M Halloran; Fiscal Year: 2006
    ..on infectiousness, VEI, of vaccination, and the analogous effects of antiviral agents on infection, AVES, disease, AVED, and infectiousness, AVEI...
  19. Oxidant induced gene expression in vitamin E deficient *
    Kishorchandra Gohil; Fiscal Year: 2002
    ..These molecular data may provide the basis for designing more effective protocols for dietary interventions with antioxidants that might ameliorate lung diseases induced by environmental oxidants. ..
  20. Vitamin E Requirements in Women,Obese Women and Diabetic Obese Women
    Maret G Traber; Fiscal Year: 2010
    ....
  21. Alpha-Tocopherol Modulation of Xenobiotic Metabolism
    Maret Traber; Fiscal Year: 2007
    ..We believe that our findings may well have a significant impact on current self-medication practices of the millions of Americans currently taking prescription drugs and vitamin E supplements. ..
  22. Mechanisms of Vitamin E Function Studied in Zebrafish
    Maret G Traber; Fiscal Year: 2010
    ..the molecular targets altered by - tocopherol-deficiency in the zebrafish will provide a solid basis for understanding why humans require -tocopherol for reproduction as well as to maintain a healthy nervous system ..
  23. Vitamin E Requirements in Women,Obese Women and Diabetic Obese Women
    Maret G Traber; Fiscal Year: 2010
    ....
  24. Mechanisms of Vitamin E Function Studied in Zebrafish
    Maret Traber; Fiscal Year: 2009
    ..the molecular targets altered by - tocopherol-deficiency in the zebrafish will provide a solid basis for understanding why humans require -tocopherol for reproduction as well as to maintain a healthy nervous system ..
  25. Alpha-Tocopherol Modulation of Xenobiotic Metabolism
    Maret Traber; Fiscal Year: 2006
    ....
  26. Oxidative Stress and Vitamin E Requirements
    Maret Traber; Fiscal Year: 2005
    ..These proposed studies will further our goal of understanding the role of vitamin E in the amelioration of oxidative damage in aging and in chronic diseases, such as heart disease, diabetes and cancer. ..
  27. Antioxidant Supplementation,Oxidative Stress & Enduranc*
    Maret Traber; Fiscal Year: 2002
    ..Finally, the investigators plan to measure markers of inflammatory response, such as C-oxidants increase and vitamin E decreases these markers. ..
  28. Alpha-Tocopherol Modulation of Xenobiotic Metabolism
    Maret Traber; Fiscal Year: 2009
    ..We believe that our findings may well have a significant impact on current self-medication practices of the millions of Americans currently taking prescription drugs and vitamin E supplements. ..