ALOXE3

Summary

Gene Symbol: ALOXE3
Description: arachidonate lipoxygenase 3
Alias: ARCI3, E-LOX, eLOX-3, eLOX3, e-LOX-3, epidermal LOX-3, epidermal lipoxygenase, epidermis-type lipoxygenase 3, hydroperoxide isomerase ALOXE3
Species: human

Top Publications

  1. ncbi Protein micelles from lipoxygenase 3
    Pierre Alexandre Brault
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biomacromolecules 3:649-54. 2002
  2. ncbi Lamellar ichthyosis
    Frank Victor
    Department of Dermatology, New York University School of Medicine, USA
    Dermatol Online J 11:13. 2005
  3. ncbi Mutation spectrum and functional analysis of epidermis-type lipoxygenases in patients with autosomal recessive congenital ichthyosis
    Katja Martina Eckl
    Cologne Center for Genomics, Division of Dermatogenetics, University of Cologne, Cologne, Germany
    Hum Mutat 26:351-61. 2005
  4. ncbi A gene cluster encoding human epidermis-type lipoxygenases at chromosome 17p13.1: cloning, physical mapping, and expression
    P Krieg
    Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany
    Genomics 73:323-30. 2001
  5. pmc ZNF750 is a p63 target gene that induces KLF4 to drive terminal epidermal differentiation
    George L Sen
    Department of Medicine Cellular and Molecular Medicine, University of California San Diego School of Medicine, La Jolla, CA 92093, USA
    Dev Cell 22:669-77. 2012
  6. doi Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation
    Peter Krieg
    Genome Modifications and Carcinogenesis, German Cancer Research Center, Heidelberg, Germany
    J Invest Dermatol 133:172-80. 2013
  7. pmc MAPK-dependent JA and SA signalling in Nicotiana attenuata affects plant growth and fitness during competition with conspecifics
    Stefan Meldau
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str, 8, Jena, D 07745, Germany
    BMC Plant Biol 12:213. 2012
  8. doi The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis
    Hao Li
    Department of Medical Sciences, Dermatology and Venereology, Uppsala University, Uppsala, Sweden
    J Invest Dermatol 132:2368-75. 2012
  9. ncbi Using nutritional indices to study LOX3-dependent insect resistance
    Cbgowda Rayapuram
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
    Plant Cell Environ 29:1585-94. 2006
  10. pmc Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia
    Ann M Gregus
    Department of Anesthesiology, University of California San Diego, La Jolla, CA 92093, USA
    FASEB J 27:1939-49. 2013

Research Grants

  1. LIPOXYGENASE CATALYSIS AND LIPID METABOLISM
    MAX FUNK; Fiscal Year: 2004
  2. Epidermal Lipoxygenase Regulation of Transcription
    BRIAN ANESKIEVICH; Fiscal Year: 2003
  3. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2006
  4. Novel Catalases and their Products
    ALAN BRASH; Fiscal Year: 2007
  5. Novel Catalases and their Products
    ALAN BRASH; Fiscal Year: 2009
  6. A Lipoxygenase Pathway in Epidermal Differentiation
    ALAN BRASH; Fiscal Year: 2009
  7. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2000
  8. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2001
  9. PATHOPHYSIOLOGICAL ROLE OF 12-R-LIPOXYGENASE
    ALAN BRASH; Fiscal Year: 2002
  10. Novel Catalases and their Products
    Alan R Brash; Fiscal Year: 2010

Scientific Experts

  • Jennifer L Moran
  • P Krieg
  • Stefan Meldau
  • Masashi Akiyama
  • Karl Gertow
  • A Vahlquist
  • Bjoern Menger
  • Izabela Buraczewska
  • Yvonne Chow
  • Ewa Skrzypczak-Jankun
  • E Skrzypczak Jankun
  • ALAN BRASH
  • Hans Torma
  • MAX FUNK
  • BRIAN ANESKIEVICH
  • Claus Schneider
  • Ian T Baldwin
  • Yuxiang Zheng
  • Zheyong Yu
  • William E Boeglin
  • Anja Paschold
  • Katja Martina Eckl
  • Cbgowda Rayapuram
  • Silvia de Juanes
  • Gerhard Furstenberger
  • Judith Fischer
  • Ann M Gregus
  • Markus Kretz
  • Nighat Sultana
  • Danielle Johnston
  • Kun Qu
  • Zurab Siprashvili
  • Paul A Khavari
  • Dan E Webster
  • George L Sen
  • Hao Li
  • Min Shen
  • Yarmilla Reinprecht
  • L Rodríguez-Pazos
  • Florence Jobard
  • Samir Anssour
  • Philip Hallenborg
  • Yigen Chen
  • Grégoire M Hummel
  • Jinsong Wu
  • Amber M Peariso
  • Santosh Nigam
  • Sebastien Fiorucci
  • Fabienne Lesueur
  • Heiko Traupe
  • Oz Barazani
  • A Kinzig
  • Frank Victor
  • Phimon Atsawasuwan
  • Max O Funk
  • Aysen Karaduman
  • Caroline Lefèvre
  • Serap Emre
  • Mark Lathrop
  • Pierre Alexandre Brault
  • Hiroo Kawajiri
  • Paul C Norris
  • Edward A Dennis
  • CAROLYN S LEE
  • Subhadra Aiyer
  • Ashley Zehnder
  • Grace X Y Zheng
  • Howard Y Chang
  • Spencer C Wei
  • Ryan A Flynn
  • Ross J Flockhart
  • Matthew W Buczynski
  • Darren S Dumlao
  • Jennifer Chow
  • Grace E Kim
  • Joanne J Steinauer
  • Arjun Raj
  • Tony L Yaksh
  • John L Rinn
  • Bethany L Fitzsimmons
  • Laura C Catella
  • Abigail F Groff
  • Ci Chu
  • Flore G Meyerstein
  • Robert C Spitale
  • Zafar Saeed Saify
  • Y Bessho
  • Brian J Zarnegar
  • Lisa D Boxer
  • Elizabeth P Loriè

Detail Information

Publications69

  1. ncbi Protein micelles from lipoxygenase 3
    Pierre Alexandre Brault
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biomacromolecules 3:649-54. 2002
    ....
  2. ncbi Lamellar ichthyosis
    Frank Victor
    Department of Dermatology, New York University School of Medicine, USA
    Dermatol Online J 11:13. 2005
    ..Mutations in genes encoding transglutaminase 1 (TGM1), the ABCA12 transporter (ABCA12), ichthyin, lipoxygenase 3 (ALOXE3), and 12(R)-lipoxygenase (ALOX12B) have been shown to underlie both NBCIE and LI.
  3. ncbi Mutation spectrum and functional analysis of epidermis-type lipoxygenases in patients with autosomal recessive congenital ichthyosis
    Katja Martina Eckl
    Cologne Center for Genomics, Division of Dermatogenetics, University of Cologne, Cologne, Germany
    Hum Mutat 26:351-61. 2005
    ..It is often associated with erythema. To date, six loci for ARCI have been mapped. Mutations in ALOXE3 and ALOX12B on chromosome 17p13, which code for two different epidermal lipoxygenases, were recently found in ..
  4. ncbi A gene cluster encoding human epidermis-type lipoxygenases at chromosome 17p13.1: cloning, physical mapping, and expression
    P Krieg
    Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany
    Genomics 73:323-30. 2001
    ..The gene encoding e-LOX-3, ALOXE3, was found to be part of a gene cluster of approximately 100 kb on human chromosome 17p13...
  5. pmc ZNF750 is a p63 target gene that induces KLF4 to drive terminal epidermal differentiation
    George L Sen
    Department of Medicine Cellular and Molecular Medicine, University of California San Diego School of Medicine, La Jolla, CA 92093, USA
    Dev Cell 22:669-77. 2012
    ..ZNF750 controlled genes mutated in numerous human skin diseases, including FLG, LOR, LCE3B, ALOXE3, and SPINK5. ZNF750 induced progenitor differentiation via an evolutionarily conserved C2H2 zinc finger motif...
  6. doi Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation
    Peter Krieg
    Genome Modifications and Carcinogenesis, German Cancer Research Center, Heidelberg, Germany
    J Invest Dermatol 133:172-80. 2013
    Loss-of-function mutations in the lipoxygenase (LOX) genes ALOX12B and ALOXE3 are the second most common cause of autosomal recessive congenital ichthyosis...
  7. pmc MAPK-dependent JA and SA signalling in Nicotiana attenuata affects plant growth and fitness during competition with conspecifics
    Stefan Meldau
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str, 8, Jena, D 07745, Germany
    BMC Plant Biol 12:213. 2012
    ....
  8. doi The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis
    Hao Li
    Department of Medical Sciences, Dermatology and Venereology, Uppsala University, Uppsala, Sweden
    J Invest Dermatol 132:2368-75. 2012
    ..Altogether, our data indicate that ichthyin and TGM1 are functionally closely related in the lipid processing and that this metabolic pathway can be modified by retinoids...
  9. ncbi Using nutritional indices to study LOX3-dependent insect resistance
    Cbgowda Rayapuram
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
    Plant Cell Environ 29:1585-94. 2006
    ..attenuata's oxylipin-mediated defenses are important for resisting attack from M. sexta larvae, and that Waldbauer nutritional assays reveal behavioural and physiological counter responses of insects to these plant defenses...
  10. pmc Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia
    Ann M Gregus
    Department of Anesthesiology, University of California San Diego, La Jolla, CA 92093, USA
    FASEB J 27:1939-49. 2013
    ..encoded by rat Alox12b and Alox12e possessed minimal 12-LOX activity with AA as substrate, while eLOX3 (encoded by Aloxe3) exhibited HXS without 12-LOX activity when coexpressed with Alox12b or supplemented with 12-HpETE...
  11. ncbi Arachidonic and linoleic acid metabolism in mouse intestinal tissue: evidence for novel lipoxygenase activity
    Hiroo Kawajiri
    Eicosanoid Biochemistry Section, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Arch Biochem Biophys 398:51-60. 2002
    ....
  12. ncbi Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17p13.1
    Florence Jobard
    Centre National de Genotypage, 91057 Evry, France
    Hum Mol Genet 11:107-13. 2002
    We report the identification of mutations in lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) genes in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17...
  13. doi Enzymatic biotransformation of terpenes as bioactive agents
    Nighat Sultana
    Pharmaceutical Research Center, PCSIR Laboratories Complex, Karachi, Pakistan
    J Enzyme Inhib Med Chem 28:1113-28. 2013
    ....
  14. pmc Control of somatic tissue differentiation by the long non-coding RNA TINCR
    Markus Kretz
    The Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 493:231-5. 2013
    ..RNA abundance of key differentiation genes, many of which are mutated in human skin diseases, including FLG, LOR, ALOXE3, ALOX12B, ABCA12, CASP14 and ELOVL3...
  15. ncbi Using 'mute' plants to translate volatile signals
    Anja Paschold
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str 8, D 07745 Jena, Germany
    Plant J 45:275-91. 2006
    ..Whether these transcriptional responses translate into changes in plant fitness in nature remains to be determined...
  16. pmc The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase
    Zheyong Yu
    Department of Pharmacology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 100:9162-7. 2003
    ..Mammalian epidermal LOX type 3 (eLOX3) is distinctive in totally lacking this typical oxygenase activity...
  17. doi Genotypic and clinical spectrum of self-improving collodion ichthyosis: ALOX12B, ALOXE3, and TGM1 mutations in Scandinavian patients
    Anders Vahlquist
    Department of Medical Sciences, Uppsala University, Uppsala, Sweden
    J Invest Dermatol 130:438-43. 2010
    ..DNA sequencing in 11 Swedish and 4 Danish SHCB patients showed ALOX12B mutations in eight cases, ALOXE3 mutations in three cases, and TGM1 mutations in one case...
  18. doi An update on molecular aspects of the non-syndromic ichthyoses
    Masashi Akiyama
    Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Exp Dermatol 17:373-82. 2008
    ....
  19. doi Moisturizers change the mRNA expression of enzymes synthesizing skin barrier lipids
    Izabela Buraczewska
    Department of Medical Sciences, Dermatology and Venereology, University Hospital, 751 85 Uppsala, Sweden
    Arch Dermatol Res 301:587-94. 2009
    ..with Hydrocarbon cream, which increased TEWL, also elevated the gene expression of GBA, SPTLC2, SMPD1, ALOX12B, ALOXE3, and HMGCS1. In addition, the expression of PPARG was decreased...
  20. doi Sodium lauryl sulphate alters the mRNA expression of lipid-metabolizing enzymes and PPAR signalling in normal human skin in vivo
    Hans Torma
    Department of Medical Sciences, Dermatology and Venereology, Akademiska sjukhuset, SE 751 85 Uppsala, Sweden
    Exp Dermatol 18:1010-5. 2009
    ..b>Epidermal lipoxygenase-3, which may generate PPARalpha agonists, exhibited reduced expression...
  21. doi Physiological, nutritional, and biochemical bases of corn resistance to foliage-feeding fall armyworm
    Yigen Chen
    Department of Entomology, University of Georgia, Griffin, GA 30223, USA
    J Chem Ecol 35:297-306. 2009
    ..Neither amino acid nor lipoxygenase 3 played a critical role in corn resistance to S. frugiperda. However, high inducibility of peroxidase may be an indicator of S. frugiperda susceptibility as observed elsewhere...
  22. doi Development of an ichthyosiform phenotype in Alox12b-deficient mouse skin transplants
    Silvia de Juanes
    Genome Modifications and Carcinogenesis, German Cancer Research Center, Heidelberg, Germany
    J Invest Dermatol 129:1429-36. 2009
    ..Genetic studies show that loss-of-function mutations in ALOX12B, encoding 12R-LOX, and in ALOXE3, encoding another closely related LOX involved in this pathway, are the second most common cause for autosomal ..
  23. doi Herbivore-induced jasmonic acid bursts in leaves of Nicotiana attenuata mediate short-term reductions in root growth
    Grégoire M Hummel
    ICG 3 Phytosphere, Research Center Julich, D 52425, Julich, Germany
    Plant Cell Environ 32:134-43. 2009
    ..Yet, inhibiting the ethylene response by applying 1-MCP led to markedly increased root growth compared with that of control plants, indicating that ethylene normally suppresses plant growth in N. attenuata seedlings...
  24. doi Molecular simulations enlighten the binding mode of quercetin to lipoxygenase-3
    Sebastien Fiorucci
    Laboratoire de Chimie des Molecules Bioactives et des Aromes, UMR CNRS 6001, Faculte des Sciences, Universite de Nice Sophia Antipolis, 06108 Nice Cedex 2, France
    Proteins 73:290-8. 2008
    ..Moreover, enzyme/substrate interactions within the cavity impose steric hindrances to quercetin that may activate a direct dioxygen addition on the substrate...
  25. doi Jasmonate perception regulates jasmonate biosynthesis and JA-Ile metabolism: the case of COI1 in Nicotiana attenuata
    Anja Paschold
    Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Hans Knoll Strasse 8, D 07745 Jena, Germany
    Plant Cell Physiol 49:1165-75. 2008
    ..Hence, in addition to its control over JA biosynthesis, COI1 might fine-tune the dynamics of the jasmonate response after induction by herbivore elicitors...
  26. doi Molecular analysis of 250 patients with autosomal recessive congenital ichthyosis: evidence for mutation hotspots in ALOXE3 and allelic heterogeneity in ALOX12B
    Katja Martina Eckl
    Division of Dermatogenetics, Cologne Center for Genomics, University of Cologne, Cologne, Germany
    J Invest Dermatol 129:1421-8. 2009
    ..We and others have shown that mutations in ALOX12B and ALOXE3, coding for the lipoxygenases 12R-LOX and eLOX-3 predominantly synthesized in the epidermis, can underlie this ..
  27. pmc Variation in antiherbivore defense responses in synthetic Nicotiana allopolyploids correlates with changes in uniparental patterns of gene expression
    Samir Anssour
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D 07745 Jena, Germany
    Plant Physiol 153:1907-18. 2010
    ..Changes in the expression of key genes and posttranscriptional events likely facilitate adaptive radiations during allopolyploid speciation events...
  28. pmc Epidermis-type lipoxygenase 3 regulates adipocyte differentiation and peroxisome proliferator-activated receptor gamma activity
    Philip Hallenborg
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
    Mol Cell Biol 30:4077-91. 2010
    ..Of the seven known murine LOXs, only the unconventional LOX epidermis-type lipoxygenase 3 (eLOX3) is expressed in 3T3-L1 preadipocytes...
  29. ncbi Updated molecular genetics and pathogenesis of ichthiyoses
    Masashi Akiyama
    Department of Dermatology, Nagoya University Graduate School of Medicine, Japan
    Nagoya J Med Sci 73:79-90. 2011
    ....
  30. doi Analysis of TGM1, ALOX12B, ALOXE3, NIPAL4 and CYP4F22 in autosomal recessive congenital ichthyosis from Galicia (NW Spain): evidence of founder effects
    L Rodríguez-Pazos
    Department of Dermatology, Faculty of Medicine, Complejo Hospitalario Universitario, SERGAS, 15782 Santiago de Compostela, Spain
    Br J Dermatol 165:906-11. 2011
    ..Mutations in six genes have been identified in autosomal recessive congenital ichthyosis (ARCI). To date, few studies have analysed the spectrum of these mutations in specific populations...
  31. pmc Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope
    Yuxiang Zheng
    Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 286:24046-56. 2011
    ..requires both the essential fatty acid linoleate and the two lipoxygenases 12R-lipoxygenase (12R-LOX) and epidermal lipoxygenase-3 (eLOX3), although their roles are poorly understood...
  32. pmc Polymorphisms in genes involved in innate immunity and susceptibility to benzene-induced hematotoxicity
    Min Shen
    Division of Cancer Epidemiology and Genetics NCI, NIH, DHHS, Bethesda MD 20892, U S A
    Exp Mol Med 43:374-8. 2011
    ..A 3-SNP block in ALOXE3 (rs7215658, rs9892383, and rs3027208) showed a global association (omnibus P = 0...
  33. doi 12- and 15-lipoxygenases in human carotid atherosclerotic lesions: associations with cerebrovascular symptoms
    Karl Gertow
    Department of Medicine, Center for Molecular Medicine, Unit of Experimental Cardiovascular Research, Karolinska Institutet, Stockholm SE 17176, Sweden
    Atherosclerosis 215:411-6. 2011
    ..was to investigate mRNA expression of 12- and 15-lipoxygenases (ALOX12, ALOX12B, ALOX15, ALOX15B) and the atypical ALOXE3 in human carotid atherosclerotic lesions, in relation to cerebrovascular symptoms and risk factors...
  34. doi Molecular basis of seed lipoxygenase null traits in soybean line OX948
    Yarmilla Reinprecht
    Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada
    Theor Appl Genet 122:1247-64. 2011
    ..The seed lipoxygenase gene mutation-based molecular markers could be used to accelerate and simplify breeding efforts for soybean cultivars with improved flavor...
  35. doi AmbLOXe--an epidermal lipoxygenase of the Mexican axolotl in the context of amphibian regeneration and its impact on human wound closure in vitro
    Bjoern Menger
    Department of Plastic, Hand, and Reconstructive Surgery, Replantation and Burn Center of Lower Saxony, Laboratory of Experimental Plastic Surgery, Hannover Medical School, Hannover, Germany
    Ann Surg 253:410-8. 2011
    ..In this study we identified an amphibian lipoxygenase and evaluated its impact on human cells in an in vitro wound model...
  36. pmc On the role of molecular oxygen in lipoxygenase activation: comparison and contrast of epidermal lipoxygenase-3 with soybean lipoxygenase-1
    Yuxiang Zheng
    Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 285:39876-87. 2010
    ..b>Epidermal lipoxygenase-3 (eLOX3) is atypical in displaying hydroperoxide isomerase activity with fatty acid hydroperoxides ..
  37. pmc Dioxygenase activity of epidermal lipoxygenase-3 unveiled: typical and atypical features of its catalytic activity with natural and synthetic polyunsaturated fatty acids
    Yuxiang Zheng
    Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 285:39866-75. 2010
    b>Epidermal lipoxygenase-3 (eLOX3) exhibits hydroperoxide isomerase activity implicated in epidermal barrier formation, but its potential dioxygenase activity has remained elusive...
  38. pmc Methyl jasmonate-elicited herbivore resistance: does MeJA function as a signal without being hydrolyzed to JA?
    Jinsong Wu
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, Jena, Germany
    Planta 227:1161-8. 2008
    ..From these results, we conclude that the resistance elicited by MeJA treatment is directly elicited not by MeJA but by its de-methylated product, JA...
  39. ncbi Hepoxilin A3 (HXA3) synthase deficiency is causative of a novel ichthyosis form
    Santosh Nigam
    Eicosanoid and Lipid Research Division, Centre for Experimental Gynecology and Breast Research, Charite, University Medical Centre Benjamin Franklin, Berlin, Germany
    FEBS Lett 582:279-85. 2008
    ..NCIE) and lamellar ichthyosis (LI) are characterized by mutations in 12R-lipoxygenase (12R-LOX) and/or epidermal lipoxygenase 3 (eLOX3) enzymes...
  40. ncbi The clinical spectrum of nonbullous congenital ichthyosiform erythroderma and lamellar ichthyosis
    M Akiyama
    Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Clin Exp Dermatol 28:235-40. 2003
    ..Mutations in any of the three known causative genes, TGM1, ALOXE3 or ALOX12B, can lead either to NBCIE or LI...
  41. ncbi Dietary supplementation with oils rich in (n-3) and (n-6) fatty acids influences in vivo levels of epidermal lipoxygenase products in guinea pigs
    C C Miller
    Department of Dermatology, University of California, Davis 95616
    J Nutr 120:36-44. 1990
    ....
  42. ncbi Three-dimensional structure of a purple lipoxygenase
    E Skrzypczak-Jankun
    Department of Chemistry, University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA
    J Am Chem Soc 123:10814-20. 2001
    ..The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity...
  43. ncbi Hes7: a bHLH-type repressor gene regulated by Notch and expressed in the presomitic mesoderm
    Y Bessho
    Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 6:175-85. 2001
    ....
  44. ncbi Volatile components in stored rice [Oryza sativa (L.)] of varieties with and without lipoxygenase-3 in seeds
    Y Suzuki
    National Agriculture Research Center, Kannondai, Tsukuba, Ibaraki 305 8666, Japan
    J Agric Food Chem 47:1119-24. 1999
    ..These results indicate that the stale flavor production in LOX-3-less rice during storage is less than that in normal LOX-3 rice...
  45. ncbi cDNA cloning, genomic structure, and chromosomal localization of a novel murine epidermis-type lipoxygenase
    A Kinzig
    Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany
    Genomics 58:158-64. 1999
    ..we isolated a cDNA encoding a novel lipoxygenase, termed epidermis-type lipoxygenase-3 (e-LOX-3, gene symbol Aloxe3), from mouse skin. Aloxe3 mRNA is expressed in the stratified epithelia of skin, tongue, and forestomach...
  46. ncbi Structural and thermochemical characterization of lipoxygenase-catechol complexes
    C Pham
    Department of Chemistry, University of Toledo, Ohio 43606, USA
    Biochemistry 37:17952-7. 1998
    ....
  47. ncbi cDNA cloning of a 8-lipoxygenase and a novel epidermis-type lipoxygenase from phorbol ester-treated mouse skin
    P Krieg
    Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1391:7-12. 1998
    ..Based on these data, we conclude that this epidermis-derived cDNA encodes a novel lipoxygenase isoform termed provisionally epidermis-type lipoxygenase 2 (e-LOX 2)...
  48. ncbi Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform
    A Kinzig
    Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    FEBS Lett 402:162-6. 1997
    ..8% identical to the ORF of an epidermal lipoxygenase gene Aloxe, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4-8]...
  49. ncbi Functional expression and cellular localization of a mouse epidermal lipoxygenase
    C D Funk
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 271:23338-44. 1996
    ..In situ hybridization revealed highly specific expression of epidermal lipoxygenase in differentiated keratinocytes of the epidermis and in restricted regions of the root sheath and bulb of ..
  50. ncbi A 96-kDa glycyrrhizin-binding protein (gp96) from soybeans acts as a substrate for casein kinase II, and is highly related to lipoxygenase 3
    K Ohtsuki
    Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences
    J Biochem 118:1145-50. 1995
    ....
  51. pmc Suppression of inflammatory responses to 12-O-tetradecanoyl-phorbol-13-acetate and carrageenin by YM-26734, a selective inhibitor of extracellular group II phospholipase A2
    A Miyake
    Molecular and Cellular Pharmacology, Dept, Yamanouchi Pharmaceutical Co Ltd, Tokyo, Japan
    Br J Pharmacol 110:447-53. 1993
    ....
  52. ncbi Position 713 is critical for catalysis but not iron binding in soybean lipoxygenase 3
    J A Kramer
    Department of Chemistry, University of Toledo, Ohio 43606
    Biochemistry 33:15017-22. 1994
    ..The possibilities that the residue at position 713 acts as a metal ligand, an acid-base catalyst, and a hydrogen bonding group are considered and discussed...
  53. ncbi Inhibition of lipoxygenase by (-)-epigallocatechin gallate: X-ray analysis at 2.1 A reveals degradation of EGCG and shows soybean LOX-3 complex with EGC instead
    Ewa Skrzypczak-Jankun
    Urology Research Center, Medical College of Ohio, Toledo, OH 43614 5807, USA
    Int J Mol Med 12:415-20. 2003
    ....
  54. ncbi Soybean lipoxygenase-3 in complex with 4-nitrocatechol
    Ewa Skrzypczak-Jankun
    Medical College of Ohio, Urology Research Center, Department of Urology, 3065 Arlington Avenue, Toledo OH 43614, USA
    Acta Crystallogr D Biol Crystallogr 60:613-5. 2004
    ..A description of the catechol binding contributes to the understanding of lipoxygenase inhibition and the participation of its co-oxidative activity in the utilization of natural flavonoids...
  55. ncbi Mung bean lipoxygenase in the production of a C6-aldehyde. Natural green-note flavor generation via biotransformation
    Yvonne Chow
    Institute of Chemical and Engineering Sciences, Jurong Island, Singapore
    Biotechnol J 2:1375-80. 2007
    ..Extraction at pH 4.5 was preferred, at which specific lipoxygenase activity was also the highest...
  56. ncbi Electrospray ionization mass spectrometry of soybean lipoxygenases: N-terminal acetylation, chemical modification, and solution conformation
    Amber M Peariso
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Proteins 70:650-8. 2008
    ..0. Remarkably, the enzyme incubated in acetic acid retained full catalytic activity...
  57. ncbi Increased SA in NPR1-silenced plants antagonizes JA and JA-dependent direct and indirect defenses in herbivore-attacked Nicotiana attenuata in nature
    Cbgowda Rayapuram
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str 8, D 07745 Jena, Germany
    Plant J 52:700-15. 2007
    ....
  58. ncbi The hepoxilin connection in the epidermis
    Alan R Brash
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232 6602, USA
    FEBS J 274:3494-502. 2007
    ..The enzymes 12R-LOX and eLOX3 are mutated in a rare form of congenital ichthyosis, and, in vitro, the two enzymes function together to convert ..
  59. ncbi Epidermal lipoxygenase products of the hepoxilin pathway selectively activate the nuclear receptor PPARalpha
    Zheyong Yu
    Division of Clinical Pharmacology, Department of Pharmacology, Vanderbilt University School of Medicine, 23rd Ave at Pierce, Nashville, TN 37232 6602, USA
    Lipids 42:491-7. 2007
    ..Functional impairment of either lipoxygenase gene (ALOX12B or ALOXE3) results in ichthyosis, suggesting a role for the common epoxyalcohol product or its metabolites in the ..
  60. ncbi A mouse mutation in the 12R-lipoxygenase, Alox12b, disrupts formation of the epidermal permeability barrier
    Jennifer L Moran
    Genetics Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Invest Dermatol 127:1893-7. 2007
    ..Identification of mutations in two lipoxygenases (LOXs), ALOX12B (12R-LOX) and ALOXE3 (eLOX3), and further functional studies implicate ALOX12B and ALOXE3 in the etiology of NCIE...
  61. pmc Sebacina vermifera promotes the growth and fitness of Nicotiana attenuata by inhibiting ethylene signaling
    Oz Barazani
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, 07745 Jena, Germany
    Plant Physiol 144:1223-32. 2007
    ..In contrast, neither inoculation nor KMnO(4) exposure influenced the germination of ir-aco seeds. We conclude that S. vermifera increases plant growth by impairing ethylene production independently of JA signaling and TPI production...
  62. ncbi Novel mutations in ALOX12B in patients with autosomal recessive congenital ichthyosis and evidence for genetic heterogeneity on chromosome 17p13
    Fabienne Lesueur
    Centre National de Genotypage, Dermatologic Disease Projects, Evry, France
    J Invest Dermatol 127:829-34. 2007
    ..chromosome 17p13, and attributed to mutations in the ALOX gene cluster, which includes three lipoxygenase genes, ALOXE3, ALOX12B, and ALOX15B...
  63. pmc Human and mouse eLOX3 have distinct substrate specificities: implications for their linkage with lipoxygenases in skin
    Zheyong Yu
    Division of Clinical Pharmacology, Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Arch Biochem Biophys 455:188-96. 2006
    Genetic and biochemical evidence suggests a functional link between human 12R-lipoxygenase (12R-LOX) and epidermal lipoxygenase-3 (eLOX3) in normal differentiation of the epidermis; LOX-derived fatty acid hydroperoxide is isomerized by ..
  64. ncbi Effect of crystal freezing and small-molecule binding on internal cavity size in a large protein: X-ray and docking studies of lipoxygenase at ambient and low temperature at 2.0 A resolution
    E Skrzypczak-Jankun
    Urology Research Center, Medical University of Ohio, Toledo, OH 43614, USA
    Acta Crystallogr D Biol Crystallogr 62:766-75. 2006
    ....
  65. ncbi Harlequin ichthyosis and other autosomal recessive congenital ichthyoses: the underlying genetic defects and pathomechanisms
    Masashi Akiyama
    Department of Dermatology, Hokkaido University Graduate School of Medicine, North 15 West 7, Sapporo 060 8638, Japan
    J Dermatol Sci 42:83-9. 2006
    ..The five genes are transglutaminase 1 gene (TGM1), ABCA12, two lipoxygenase genes, ALOXE3 and ALOX12B and ichthyin...
  66. ncbi Expression of lysyl oxidase isoforms in MC3T3-E1 osteoblastic cells
    Phimon Atsawasuwan
    CB 7455, Dental Research Center, University of North Carolina at Chapel Hill, NC 27599 7455, USA
    Biochem Biophys Res Commun 327:1042-6. 2005
    ..This indicates that the expression of LOX and its isoforms is highly regulated during osteoblast differentiation, suggesting their distinct roles in collagen matrix stabilization and subsequent mineralization...
  67. ncbi Mutations associated with a congenital form of ichthyosis (NCIE) inactivate the epidermal lipoxygenases 12R-LOX and eLOX3
    Zheyong Yu
    Department of Pharmacology, Vanderbilt University School of Medicine, 23rd Ave at Pierce, Nashville, TN 37232 6602, USA
    Biochim Biophys Acta 1686:238-47. 2005
    ..Genetic studies indicated that 12R-lipoxygenase (12R-LOX) or epidermal lipoxygenase-3 (eLOX3) was mutated in six families affected by NCIE [F. Jobard, C. Lefevre, A. Karaduman, C...
  68. ncbi Pharmacology of the dual inhibitor of cyclooxygenase and 5-lipoxygenase 3-hydroxy-5-trifluoromethyl-N-(2-(2-thienyl)-2-phenyl-ethenyl)-benzo (b)thiophene-2-carboxamide
    P J Bailey
    Department of Biochemistry Molecular Biology, Merck Sharp and Dohme Research Laboratories, Rahway, NJ
    Arzneimittelforschung 38:372-8. 1988
    ..The ulcerogenicity and gastric bleeding induced by L-652,343 is extremely low, providing a favorable therapeutic index which is superior to that of indomethacin, piroxicam and phenylbutazone...

Research Grants32

  1. LIPOXYGENASE CATALYSIS AND LIPID METABOLISM
    MAX FUNK; Fiscal Year: 2004
    ..4. To conduct recombinant modifications of lipoxygenase-3 in order to determine domain interactions, as well as to identify specific amino acid side chains that are necessary for substrate recognition and catalysis. ..
  2. Epidermal Lipoxygenase Regulation of Transcription
    BRIAN ANESKIEVICH; Fiscal Year: 2003
    ....
  3. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2006
    ..These studies will develop new insights into the biochemistry of mammalian cyclooxygenases and as a consequence have practical benefits in development of therapeutics. ..
  4. Novel Catalases and their Products
    ALAN BRASH; Fiscal Year: 2007
    ..The results of this study will provide new insights and a new way of thinking about the enzymatic capabilities of a long-recognized protein family with established roles as a sentinel at the forefront of oxidative defense. ..
  5. Novel Catalases and their Products
    ALAN BRASH; Fiscal Year: 2009
    ..The results of this study will provide new insights and a new way of thinking about the enzymatic capabilities of a long-recognized protein family with established roles as a sentinel at the forefront of oxidative defense. ..
  6. A Lipoxygenase Pathway in Epidermal Differentiation
    ALAN BRASH; Fiscal Year: 2009
    ..The genetics identifies both 12R-LOX and eLOX3 (epidermal lipoxygenase-3) as defective in the families studied, and by implication, as playing a critical and important role in ..
  7. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2000
    ..The results of this study will help elucidate the molecular basis for the specificity of oxygenation by different lipoxygenases and cyclooxygenases and characterize an important target for known or potential therapeutic interventions. ..
  8. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2001
    ..Finally, in the theme of novel oxygenations we will characterize novel lipoxygenase activities in animal models of vascular tone and cell proliferation. ..
  9. PATHOPHYSIOLOGICAL ROLE OF 12-R-LIPOXYGENASE
    ALAN BRASH; Fiscal Year: 2002
    ..abstract_text> ..
  10. Novel Catalases and their Products
    Alan R Brash; Fiscal Year: 2010
    ..By uncovering the details of how different enzymes deal with oxidants and how they relate or differ from each other this project will improve our understanding of oxidative stress, which will ultimately help control it. ..