Research Topics

Genomes and Genes

AKT1

Summary

Gene Symbol: AKT1
Description: v-akt murine thymoma viral oncogene homolog 1
Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Alias: AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1 kinase, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha
Species: human
Products:     AKT1 antibody    AKT1 chemical    AKT1 cDNA    AKT1 ELISA    AKT1 protein    AKT1 siRNA    AKT1 other

Webpages

  1. insulin
    student.biology.arizona.edu/honors2003/group05/bg.html
  2. medical college of georgia :: vascular biology center :: biography : david fulton
    www.mcg.edu/centers/VBC/vbc_bio_dfulton.html
  3. ipr017441 protein kinase, atp binding site
    www.ebi.ac.uk/interpro/IEntry?ac=IPR017441
  4. ipr002290 serine/threonine protein kinase
    www.ebi.ac.uk/interpro/IEntry?ac=IPR002290
  5. faculty biography for leonidas c. platanias, md
    www.medicine.northwestern.edu/scripts/bio.pl?pid=609
  6. systems biology at harvard medical school
    sysbio.med.harvard.edu/faculty/cantley/
  7. proteomics facility - publications - md anderson cancer center
    www.mdanderson.org/education-and-research/resources-for-prof ...
  8. e-prints soton - hepatitis c virus ns5a-mediated activation of phosphoinositide 3-kinase results in stabilization of cellular beta-catenin and stimulation of beta-catenin-responsive transcription
    eprints.soton.ac.uk/27444/
  9. cdfd :: immunology :: publications
    www.cdfd.org.in/labpages/immunology_publications.html
  10. spinal cord and brain injury research center -pamela knapp
    www.mc.uky.edu/scobirc/faculty/knapp.html

Research Grants

  1. Structure And Function Of Peptide And Protein Hormones
    HAO CHIA CHEN; Fiscal Year: 2005
  2. Regulation of the Hypothalamic GnRH Neuron
    Kevin J Catt; Fiscal Year: 2006
  3. Molecular Regulation and Biological Functions of PIKE-A
    Keqiang Ye; Fiscal Year: 2008
  4. Role of Integrin-Linked Kinase in Heart Failure and Ischemic Injury
    Anthony Rosenzweig; Fiscal Year: 2008
  5. Molecular Mechanisms Of Growth Control And Carcinogenesi
    J Silvio Gutkind; Fiscal Year: 2006
  6. Regulation of Akt and mitochondrial function
    Joan Heller Brown; Fiscal Year: 2008
  7. Expression, Structure/function And Regulation Of Phospho
    Vincent Manganiello; Fiscal Year: 2006
  8. Novel Targeted Therapy for CLL
    Dale J Christensen; Fiscal Year: 2008
  9. Oral Carcinogenesis
    J Silvio Gutkind; Fiscal Year: 2006
  10. Angiotensin II Receptors And Signaling Mechanisms
    Kevin J Catt; Fiscal Year: 2006

Publications

  1. Okadaic acid inhibits insulin-induced glucose transport in fetal brown adipocytes in an Akt-independent and protein kinase C zeta-dependent manner
    A M Valverde
    Departamento de Bioquímica y Biología Molecular, Centro Mixto CSIC/UCM, Facultad de Farmacia, Universidad Complutense, 28040, Madrid, Spain
    FEBS Lett 472:153-8
  2. Defective insulin-induced GLUT4 translocation in skeletal muscle of high fat-fed rats is associated with alterations in both Akt/protein kinase B and atypical protein kinase C (zeta/lambda) activities
    F Tremblay
    Department of Physiology, Laval University Hospital Research Center, Quebec, Canada
    Diabetes 50:1901-10
  3. Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes
    R Hernandez
    Departamento de Bioquimica y Biologia Molecular II, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    FEBS Lett 494:225-31
  4. The small GTP-binding protein rho activates c-Jun N-terminal kinases/stress-activated protein kinases in human kidney 293T cells. Evidence for a Pak-independent signaling pathway
    H Teramoto
    Laboratory of Cellular Development and Oncology, NIDR, National Institutes of Health, Bethesda, Maryland 20892 4330, USA
    J Biol Chem 271:25731-4
  5. Role of three FKHR phosphorylation sites in insulin inhibition of FKHR action in hepatocytes
    A O Scheimann
    Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA
    Horm Metab Res 33:631-8
  6. Selective small-molecule inhibitors of glycogen synthase kinase-3 activity protect primary neurones from death
    D A Cross
    Neurology Centre of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Harlow, Essex, UK
    J Neurochem 77:94-102
  7. Inhibition of GSK-3 selectively reduces glucose-6-phosphatase and phosphatase and phosphoenolypyruvate carboxykinase gene expression
    P A Lochhead
    Division of Cell Signalling, School of Life Sciences, University of Dundee, UK
    Diabetes 50:937-46
  8. Insulin activates protein kinases C-zeta and C-lambda by an autophosphorylation-dependent mechanism and stimulates their translocation to GLUT4 vesicles and other membrane fractions in rat adipocytes
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:25308-16
  9. Protein kinase C isoforms involved in the transcriptional activation of cyclin D1 by transforming Ha-Ras
    S Kampfer
    Institute of Medical Chemistry and Biochemistry, University of Innsbruck, A-6020 Innsbruck, Austria
    J Biol Chem 276:42834-42
  10. Okadaic acid activates atypical protein kinase C (zeta/lambda) in rat and 3T3/L1 adipocytes. An apparent requirement for activation of Glut4 translocation and glucose transport
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:14074-8

Scientific Experts

Detail Information

Webpages62 found, 30 most recent shown here

  1. insulin
    student.biology.arizona.edu/honors2003/group05/bg.html
  2. medical college of georgia :: vascular biology center :: biography : david fulton
    www.mcg.edu/centers/VBC/vbc_bio_dfulton.html
  3. ipr017441 protein kinase, atp binding site
    www.ebi.ac.uk/interpro/IEntry?ac=IPR017441
  4. ipr002290 serine/threonine protein kinase
    www.ebi.ac.uk/interpro/IEntry?ac=IPR002290
  5. faculty biography for leonidas c. platanias, md
    www.medicine.northwestern.edu/scripts/bio.pl?pid=609
  6. systems biology at harvard medical school
    sysbio.med.harvard.edu/faculty/cantley/
  7. proteomics facility - publications - md anderson cancer center
    www.mdanderson.org/education-and-research/resources-for-prof ...
  8. e-prints soton - hepatitis c virus ns5a-mediated activation of phosphoinositide 3-kinase results in stabilization of cellular beta-catenin and stimulation of beta-catenin-responsive transcription
    eprints.soton.ac.uk/27444/
  9. cdfd :: immunology :: publications
    www.cdfd.org.in/labpages/immunology_publications.html
  10. spinal cord and brain injury research center -pamela knapp
    www.mc.uky.edu/scobirc/faculty/knapp.html
  11. medline publication: arsenite-induced phosphorylation of histone h3 at serine 10 is mediated by akt1, extracellular signal-regulated kinase 2, and p90 ribosomal s6 kinase 2 but not mitogen- and stress-activated protein kinase 1
    www.fred.psu.edu/ds/retrieve/fred/medlinepub/12529330
  12. cell viability, signaling & drug screening protocols index
    www.promega.com/tbs/cellprolif.htm
  13. publication: assessment of the roles of mitogen-activated protein kinase, phosphatidylinositol 3-kinase, protein kinase b, and protein kinase c in insulin inhibition of camp-induced phosphoenolpyruvate carboxykinase gene transcription
    www.fred.psu.edu/ds/retrieve/fred/publication/9668048
  14. renal physio : main
    physiologie.i-med.ac.at/pfaller/index.php
  15. craig henke, md - med - dom - pulmonary allergy sleep med, university of minnesota
    www.med.umn.edu/pacc/faculty/henke/home.html
  16. k­z
    www.cgu.edu.tw/DOP/teacher/cmy.htm
  17. research awards 2004 october - cornell university college of veterinary medicine
    www.vet.cornell.edu/research/awards/04October/Elble.htm
  18. urology research: mechanisms for acquired changes in prostate growth regulation
    www.surgery.wisc.edu/urology/research/grant_tj_dod.shtml
  19. ucl chemistry - academic staff - dr helen hailes
    www.chem.ucl.ac.uk/people/hailes/index.html
  20. gene names | caenorhabditis genetics center
    biosci.umn.edu/CGC/nomenclature/genes.html
  21. carol a. lange, ph.d
    www.micab.umn.edu/faculty/Lange.html
  22. laboratory of intracellular communications
    www.img.cas.cz/lic/index.html
  23. department of cellular signaling
    www2.iib.uam.es/signal
  24. faculty - cancer biology graduate program - university of colorado denver
    www.uchsc.edu/expath/faculty.htm
  25. references  (signal transduction and activation of the nadph oxidase in eosinophils
    memorias.ioc.fiocruz.br/92sup2/3466ref.html
  26. department of clinical biochemistry, university of cambridge
    www.clbc.cam.ac.uk/pages/mrcphd.html
  27. search results for hotels wicklow - ireland.com
    www.ireland.com/travelnow/search.ie?hid=eNorSi0uzSkpds7PK0mt ...
  28. medizinische universitaet graz :: forschungszentrum der uni-kliniken graz zmf/fug
    www.meduni-graz.at/zmf/projekte/117.htm
  29. university of florida shands cancer center: epidermal growth factor receptor expression important for response to tarceva® in gliomas
    ufscc.ufl.edu/Professional/cancernews.aspx?section=cancernew ...

Research Grants95

  1. Structure And Function Of Peptide And Protein Hormones
    HAO CHIA CHEN; Fiscal Year: 2005
    ..The increased levels of PKB/Akt and GSK3beta phosphorylation at Ser9 are noteworthy...
  2. Regulation of the Hypothalamic GnRH Neuron
    Kevin J Catt; Fiscal Year: 2006
    ..Consistent with the prosurvival and mitogenic effects of PI 3-kinase/Akt (PI3K/Akt) downstream of the EGF-R, inhibition of PI3K diminished the activation of these proteins following ..
  3. Molecular Regulation and Biological Functions of PIKE-A
    Keqiang Ye; Fiscal Year: 2008
    ..by applicant): PIKE (PI 3-Kinase Enhancer) plays an essential role in mediating cell survival through PI 3-kinase/Akt signaling. Currently, three isoforms have been characterized: PIKE-L, -S and -A...
  4. Role of Integrin-Linked Kinase in Heart Failure and Ischemic Injury
    Anthony Rosenzweig; Fiscal Year: 2008
    ..During the first project period, we learned that while acute activation of the serine-threonine kinase, Akt, is cardioprotective, chronic activation of Akt has deleterious effects associated with feedback inhibition of ..
  5. Molecular Mechanisms Of Growth Control And Carcinogenesi
    J Silvio Gutkind; Fiscal Year: 2006
    ..by activating the beta-catenin pathway through a novel signaling route that involves the activation of PI3K and AKT by free Gbeta-gamma subunits, and the direct association of Galpha with the RGS domain of Axin...
  6. Regulation of Akt and mitochondrial function
    Joan Heller Brown; Fiscal Year: 2008
    ..In the heart, as in isolated cardiomyocytes, the serine threonine kinase Akt protects against I/R injury and apoptosis induced by various cell stresses...
  7. Expression, Structure/function And Regulation Of Phospho
    Vincent Manganiello; Fiscal Year: 2006
    ..receptor substrate-1 (IRS-1), and activated IRS-1-associated phosphatidylinositol 3-kinase (P13-K) and protein kinase B (PKB) in both intracellular membrane and cytosolic fractions...
  8. Novel Targeted Therapy for CLL
    Dale J Christensen; Fiscal Year: 2008
    ..evaluating the signaling pathways that are aberrantly activated in B-CLL cells, it was demonstrated that the kinase Akt is constitutively activated and the overstimulation of Akt results in activation of the NFkB pathway that leads to ..
  9. Oral Carcinogenesis
    J Silvio Gutkind; Fiscal Year: 2006
    ..networks in HNSCC: novel mechanism-based approaches for HSNCC treatment Recent findings suggest that the ability of Akt to coordinate mitogenic signaling with nutrient-sensing pathways controlling protein synthesis may represent an ..
  10. Angiotensin II Receptors And Signaling Mechanisms
    Kevin J Catt; Fiscal Year: 2006
    ..and epsilon, caused phosphorylation of Src (at Tyr416), proline-rich tyrosine kinase (Pyk2 at Tyr402), EGF-R, protein kinase B/Akt, ERK1/2, and their dependent protein, p90 ribosomal S6 kinase...
  11. PHOSPHORYLATION IN RENAL CELL INJURY
    Goutam Ghosh Choudhury; Fiscal Year: 2004
    ..A serine threonine kinase, Akt, has been identified as a downstream target of PI 3K...
  12. Regulation of Mixed Lineage Kinase 3 by the tumor suppressor protein Merlin
    Deborah N Chadee; Fiscal Year: 2008
    ..b>Rac and Cdc42, small GTPases of the Rho family of proteins, are critical regulators of the cytoskeleton and Merlin has ..
  13. Regulation And Processing Of Amyloid Precursor Protein G
    John W Kusiak; Fiscal Year: 2002
    ..We have focused on mitogen-activated protein kinases and the PI-3 kinase/Akt cascades...
  14. The role of Akt in cell survival and cell growth
    Nissim Hay; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): The serine/threonine kinase Akt/PKB plays a critical role in executing multiple cellular metabolic pathways and in particular cellular energy metabolism...
  15. Ovarian Follicular Developmental and Hormone Action
    JoAnne S Richards; Fiscal Year: 2007
    ..Specifically, FSH impacts the PI3-kinase, PKB/AKT cell survival pathway in several ways...
  16. Role of protein kinase Akt in cerebral ischemia
    JAMES KUANG-JAN LIAO; Fiscal Year: 2008
    b>Protein kinase B/Akt is a serine-threonine kinase which acts downstream of phosphatidylinositol 3-kinase (PI3K) and is induced by many endogenous ligands such as IGF-1, FGF, steroid hormones, and statins...
  17. P13K/AKT crosstalk with ER-signaling and cell survival
    MATTHEW EDWARD BUROW; Fiscal Year: 2008
    DESCRIPTION (provided by applicant): The primary long-term objective of this research is to understand the role of AKT-signaling cross-talk with the estrogen receptor (ER) in the control of cell survival and in the development of anti-..
  18. Novel Inhibitors of Akt Signaling in Pancreatic Cancer
    Garth Powis; Fiscal Year: 2007
    ..Akt (protein kinase B) is a PH domain containing serine/threonine kinase that is a key component of the PtdIns-3-kinase cell ..
  19. Novel Inhibitors of Akt Signaling in Pancreatic Cancer
    Garth Powis; Fiscal Year: 2008
    ..Akt (protein kinase B) is a PH domain containing serine/threonine kinase that is a key component of the PtdIns-3-kinase cell ..
  20. Phosphorylation and Endothelial Nitric Oxide Production
    William C Sessa; Fiscal Year: 2008
    ..the role if specific phosphorylation site, serine 1179 in regulating eNOS function and that the protein kinase Akt1 is necessary for post-natal angiogenesis in response to hindlimb ischemia or to exogenous administration of ..
  21. The Role of Osteopontin in Cancer Progression and Bone Metastasis
    Brian Robertson; Fiscal Year: 2008
    ..A key step in elucidating the role of OPN in cancer came when it was shown to increase the activation of PKB/Akt, an important molecule for regulating cell survival and progression...
  22. NITROGEN HETEROCYCLES: METABOLIC EFFECTS AND TOXICITY
    Raymond F Novak; Fiscal Year: 2004
    ..Mechanistic studies implicate the Src kinase, PI 3-kinase, Akt kinase/Protein kinase B, p70 S6 kinase signaling cascade in regulation of CYP2E1 expression and polyribosomal distribution, and mRNA ..
  23. The Role of Osteopontin in Cancer Progression and Bone Metastasis
    Brian Robertson; Fiscal Year: 2007
    ..A key step in elucidating the role of OPN in cancer came when it was shown to increase the activation of PKB/Akt, an important molecule for regulating cell survival and progression...
  24. Role of Pak kinases in survival and motility
    JEFFREY M FIELD; Fiscal Year: 2007
    ..We have been studying the role of Akt in cell migration and have implicated Pak in the process. Interestingly, the Akt1 and Akt2 isoforms have opposing roles in cell migration and regulation of Pak...
  25. Role of Pak kinases in survival and motility
    JEFFREY M FIELD; Fiscal Year: 2008
    ..We have been studying the role of Akt in cell migration and have implicated Pak in the process. Interestingly, the Akt1 and Akt2 isoforms have opposing roles in cell migration and regulation of Pak...
  26. Regulation of Cell Survival by the Rapamycin-Insensitive mTOR Complex
    Estela Jacinto; Fiscal Year: 2008
    ..This novel function of mTORC2 involves phosphorylation of Akt/PKB and is critical only under stress conditions. We have identified SIN1 as an integral component of mTORC2...
  27. Molecular Mechanisms Of Synapse Development And Plastici
    Bai Lu; Fiscal Year: 2006
    ..Second, Akt, the downstream target of PI3 kinase, is necessary for the long-term but not acute effects...
  28. Molecular Aspects of Insulin Receptor Signaling
    Michel Bernier; Fiscal Year: 2006
    ..Interestingly, MEF PLCg1-/- cells remain very sensitive to insulin with regard to AKT phosphorylation...
  29. Regulation of MEKK3 by Oxidative Stress
    Richard R Vaillancourt; Fiscal Year: 2005
    ..The Akt signal transduction pathway is regulated by ROS in vascular smooth muscle cells treated with angiotensin II, PDGF, ..
  30. Molecular Pharmacology of Insulin Resistance in Burns
    JEEVENDRA MARTYN; Fiscal Year: 2008
    ..the insulin receptor, insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase (PI3-K), Akt/PKB (protein kinase B), and glycogen synthase kinase-32 (GSK-32)...
  31. Cell and Molecular Genetic Analysis of Pediatric Tumors
    CAROL THIELE-GALETTO; Fiscal Year: 2006
    ..Using genetic approaches we found that activation of AKT can substitute for BDNF and rescue NB cells from death induced by cisplatin and etoposide while a dominant negative ..
  32. Regulation of apoptosis by PI 3-kinase/Akt signaling
    Geoffrey M Cooper; Fiscal Year: 2008
    ..by applicant): The overall goal of the proposed research is to elucidate the mechanisms by which the PI 3-kinase/Akt/GSK-3 signaling pathway regulates cell growth and survival...
  33. Akt-regulated pathways in platelet function
    Donna S Woulfe; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): The goal of this proposal is to better define the roles of Akt in platelet signaling and thrombosis...
  34. Acute Nutritional Deprivation: Diaphragm Influences
    MICHAEL IAN LEWIS; Fiscal Year: 2007
    ..Hypothesis 1: Down-regulation of the PI3K/Akt/mTOR signal transduction pathway is an important determinant of disordered synthesis of muscle proteins and atrophy ..
  35. AKT MEDIATED TAMOXIFEN RESISTANCE IN BREAST CANCER CELLS
    Harikrishna Nakshatri; Fiscal Year: 2005
    ..We have discovered that the growth factor-regulated serine/threonine kinase AKT phosphorylates ERalpha in vitro and permits activation of ERalpha in the absence of estrogen...
  36. Structure Based Discovery of AKT Inhibitors
    Robert I Glazer; Fiscal Year: 2003
    DESCRIPTION (provided by applicant): AKT1 (protein kinase B) is the cellular homolog of the v-akt oncogene, and represents one of three isoforms of a multigene protein-serine/threonine kinase family...
  37. Chimaerins:new receptors for DIACYGLY & phorbol esters
    Marcelo G Kazanietz; Fiscal Year: 2007
    ..Indeed, we have established that "chimaerins", a family of proteins with homology to Rac GAPs (GTPase Activating Proteins) are high affinity receptors for phorbol esters and DAG...
  38. Regulation of TGFBeta-induced apoptosis in liver cells
    Kunxin Luo; Fiscal Year: 2008
    ..In a search for molecules that interact with Smad3, the P.I. identified Akt, a critical molecule functioning in the cell survival pathway downstream of insulin...
  39. Regulation of TGFBeta-induced apoptosis in liver cells
    Kunxin Luo; Fiscal Year: 2007
    ..In a search for molecules that interact with Smad3, the P.I. identified Akt, a critical molecule functioning in the cell survival pathway downstream of insulin...
  40. Myocardial ischemic activation of the Akt pathway
    Stephen C Armstrong; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): The PI-3 kinase/Akt pathway is a potential mediator of the cardioprotection provided by preconditioning...
  41. Regulation of Cellular Survival, Size and Metabolism
    David R Plas; Fiscal Year: 2007
    ..have been shown to prevent cell death are the pro-survival Bcl-2 family members, and the serine-threonine kinase Akt. Previous work with Akt has demonstrated that cells surviving in an Akt-dependent manner are characterized by ..
  42. Akt-Regulated Gene Expression in Bladder Smooth Muscle
    Rosalyn M Adam; Fiscal Year: 2005
    DESCRIPTION (provided by applicant): The aim of this project is to identify Akt target genes in bladder smooth muscle cells (SMC) exposed to hypertrophic signals...
  43. Mild Hypothermic Neuroprotection for Cerebral Ischemia
    Gary K Steinberg; Fiscal Year: 2008
    ..In particular, we will study the serine/threonine kinase Akt/PKB (or "Akt") survival pathway, examining how it is modulated by hypothermia, how such modulation correlates with ..
  44. Evaluation of a novel AKT inhibitor in ovarian cancer
    Jiayuh Lin; Fiscal Year: 2005
    ..AKT or Protein Kinase B (PKB) is a serine/threonine kinase that is activated in response to growth factor or cytokine...
  45. AKT PROTOONCOGENE IN BREAST CANCER
    Robert I Glazer; Fiscal Year: 2003
    This proposal will address the hypothesis that AKT1, the cellular homolog of the protein-serine/threonine kinase oncogene v-akt, plays a key role in the proliferation, survival and etiology of breast cancer...
  46. IP3 Receptor Phosphorylation by Akt Kinase
    Suresh K Joseph; Fiscal Year: 2006
    ..The multifunctional serine/threonine protein kinase Akt acts downstream of the lipid kinase phosphoinositide 3-kinase and functions as an essential mediator in many ..
  47. Mechanisms of Akt-induced mayocardial contraction
    Gianluigi Condorelli; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): Our laboratory has determined that the serine/threonine kinase Akt, a key downstream component of the IGF1/PI3K pathway, has a profound effect on cardiac function both in vivo and in vitro...
  48. Mechanisms of Akt-induced mayocardial contraction
    Gianluigi Condorelli; Fiscal Year: 2008
    DESCRIPTION (provided by applicant): Our laboratory has determined that the serine/threonine kinase Akt, a key downstream component of the IGF1/PI3K pathway, has a profound effect on cardiac function both in vivo and in vitro...
  49. P38 MAP Kinase and P1-3 Kinase for Chemoprevention of UVB-Induced Skin Cancer
    G T Bowden; Fiscal Year: 2008
    ..by applicant): The hypothesis to be tested in this Project is that the p38 MAP kinase and the PI-3-kinase, Akt as well as EGFR pathway are significant targets for the development of chemoprevention agents to inhibit UV induced ..
  50. Effects of Akt activation in ischemia-reperfusion injury
    Anthony Rosenzweig; Fiscal Year: 2005
    ..We found that the serine-threonine kinase, Akt, is a critical mediator of IGF-I-induced survival signaling in cardiomyocytes in acute models of transient ischemia ..
  51. Regulation of NF-kB by the Akt kinase
    Lawrence P Kane; Fiscal Year: 2008
    ..I have been studying the role of the serine/threonine kinase Akt in cellular activation and NF-?B induction...
  52. Intracellular pathways of monocyte survival
    Clay B Marsh; Fiscal Year: 2007
    ..The serine threonine kinase Akt1 is important in cellular survival and differentiation...
  53. Regulation of NF-kB by the Akt kinase
    Lawrence P Kane; Fiscal Year: 2007
    ..I have been studying the role of the serine/threonine kinase Akt in cellular activation and NF-?B induction...
  54. Intracellular pathways of monocyte survival
    Clay B Marsh; Fiscal Year: 2008
    ..The serine threonine kinase Akt1 is important in cellular survival and differentiation...
  55. Fluorescent Activity Sensors in Analysis of Oncogenic P13K/Akt Signaling
    Jin Zhang; Fiscal Year: 2006
    ..of phosphatidylinositol 3-kinase (PI3K) and the downstream serine/threonine kinase Akt (also known as protein kinase B) triggers a cascade of responses that are critical for tumorigenesis, from cell growth and proliferation to ..
  56. Fluorescent Activity Sensors in Analysis of Oncogenic P13K/Akt Signaling
    Jin Zhang; Fiscal Year: 2007
    ..of phosphatidylinositol 3-kinase (PI3K) and the downstream serine/threonine kinase Akt (also known as protein kinase B) triggers a cascade of responses that are critical for tumorigenesis, from cell growth and proliferation to ..
  57. The Role of MIM in growth factor signaling and cell motility
    STEVEN ZHAN; Fiscal Year: 2008
    ..on these data, we hypothesize that MIM is implicated in a novel signaling pathway antagonizing the function of Rac in the assembly of actin cytoskeleton, the activity that is subjected to a regulation through tyrosine ..
  58. MMPs and Synaptic Injury with HIV/METH
    KATHERINE E CONANT; Fiscal Year: 2008
    ..Public Health Relevance: This Public Health Relevance is not available...
  59. Sphingosine-1-phosophate signaling in vasculogenesis
    Kelley M Argraves; Fiscal Year: 2008
    ..Determine the significance S1P signaling to adult vasculogenesis mediated by circulating angioblasts. Public Health Relevance: This Public Health Relevance is not available...
  60. Pathologic and therapeutic implications of Akt attenuation in TSC cells and tumor
    Brendan D Manning; Fiscal Year: 2008
    ..this elevated mTOR activity triggers a negative feedback loop in TSC-deficient cells that renders the PI3K-Akt pathway unresponsive to specific growth factors...
  61. Role of Prostaglandins & Phospholipase A in melanocytes
    Glynis A Scott; Fiscal Year: 2007
    ..We propose that these receptors (EP1, EP3 and FP) activate Rac/Cdc42 and or inhibit Rho, which we have shown in the first funding period mediate HM dendricity and MS transfer...
  62. Receptor Tyrosine Kinases in Mechanotransduction
    Zheng-Gen Jin; Fiscal Year: 2007
    ..In this pathway, VEGFR2 may emerge as a central mediator for laminar flow atheroprotective effects, which will provide new insight into the prevention of atherosclerotic vascular disease...
  63. Pathologic and therapeutic implications of Akt attenuation in TSC cells and tumor
    Brendan D Manning; Fiscal Year: 2007
    ..this elevated mTOR activity triggers a negative feedback loop in TSC-deficient cells that renders the PI3K-Akt pathway unresponsive to specific growth factors...
  64. Receptor Tyrosine Kinases in Mechanotransduction
    Zheng-Gen Jin; Fiscal Year: 2008
    ..Public Health Relevance: This Public Health Relevance is not available...
  65. MMP-7 and SNARE Cleavage in Neuroinflammation
    KATHERINE E CONANT; Fiscal Year: 2008
    ..Excess levels of select proteases may be considered as novel targets for neuro-protective drugs. Public Health Relevance: This Public Health Relevance is not available...
  66. Chaperone-mediated signaling in Alzheimer's disease
    Chad A Dickey; Fiscal Year: 2008
    ..This abstract is not available. Public Health Relevance: This Public Health Relevance is not available...
  67. Modulation of Neutrophil Apoptosis by Akt-Hsp27 Signalosome
    Madhavi J Rane; Fiscal Year: 2008
    ..The broad long-term objective of our laboratory is to elucidate the role of Akt in regulating PMN functions...
  68. Role of Prostaglandins & Phospholipase A in melanocytes
    Glynis A Scott; Fiscal Year: 2008
    ..We propose that these receptors (EP1, EP3 and FP) activate Rac/Cdc42 and or inhibit Rho, which we have shown in the first funding period mediate HM dendricity and MS transfer...
  69. Mechanochemical regulation of endothelial permeability
    Konstantin G Birukov; Fiscal Year: 2007
    ..1-phosphate) and barrier-disruptive (thrombin) agents and described a critical involvement of small GTPases Rac and Rho in remodeling of cell focal adhesions and adherens junctions associated with EC barrier regulation...
  70. Chemokine Receptor Signaling in HIV Neuropathology
    Marcus Kaul; Fiscal Year: 2007
    ..Activation of p38 MAPK, JNK, ERK, Akt and STAT1 will be monitored by immunoblotting, immunocomplex protein kinase assay, and immunofluorescence...
  71. Neurogenesis in the Prostate Cancer Microenvironment
    GUSTABO GUSTAVO; Fiscal Year: 2007
    ..abstract not provided..
  72. MMPs and Synaptic Injury with HIV/METH
    KATHERINE E CONANT; Fiscal Year: 2007
    ....
  73. Chaperone-mediated signaling in Alzheimer's disease
    Chad A Dickey; Fiscal Year: 2007
    ..Specifically, we intend to focus on the removal of the proteins once they have already started to accumulate in an effort to reverse the progression of the disease rather than prevent it...
  74. Thrombin signaling and HIV dementia
    KATHERINE E CONANT; Fiscal Year: 2006
    ....
  75. Therapeutic Intervention for Tauopathies
    Chad A Dickey; Fiscal Year: 2006
    ..Those compounds with the greatest promise based on in vitro analyses will then be used in animal models for tauopathy that are exclusive to the Mayo Clinic...
  76. MMPs in neural networking
    KATHERINE E CONANT; Fiscal Year: 2006
    ..We will also test the hypothesis that such effects are dependent on neuronal integrins. The results of our studies should improve our understanding of those CNS inflammatory diseases that are characterized by elevated levels of MMPs...
  77. Effects of mTOR inhibitors on multiple myeloma tumors
    PATRICK J FROST; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): The AKT signaling pathway is important for the survival of multiple myeloma cells (MM)...
  78. Crosstalk TGFbeta and HER2 (ErbB2) Signaling in Mammary Tumorigenesis
    Shizhen Emily Wang; Fiscal Year: 2007
    ..Specific aim 2: to determine whether the Rac/Pak pathway is required for the invasiveness and survival mediated by the interaction between HER2 and TGF?, ..
  79. HIV 1 PROTEINS AND DRUGS OF ABUSE AND METALLOPROTEASES
    KATHERINE E CONANT; Fiscal Year: 2003
    ..We will also examine the mechanisms by which these substances may affect MMP production, and identify clinically tolerable compounds which may inhibit such production...
  80. Crosstalk TGFbeta and HER2 (ErbB2) Signaling in Mammary Tumorigenesis
    Shizhen Emily Wang; Fiscal Year: 2008
    ..Specific aim 2: to determine whether the Rac/Pak pathway is required for the invasiveness and survival mediated by the interaction between HER2 and TGF?, ..
  81. Therapeutic Intervention for Tauopathies
    Chad A Dickey; Fiscal Year: 2007
    ..Those compounds with the greatest promise based on in vitro analyses will then be used in animal models for tauopathy that are exclusive to the Mayo Clinic. Public Health Relevance: This Public Health Relevance is not available...
  82. Sphingosine-1-phosophate signaling in vasculogenesis
    Kelley M Argraves; Fiscal Year: 2007
    ..Characterize the involvement of integrins in S1P-induced angioblast motility, and 3. Determine the significance S1P signaling to adult vasculogenesis mediated by circulating angioblasts...
  83. Effects of mTOR inhibitors on multiple myeloma tumors
    PATRICK J FROST; Fiscal Year: 2008
    DESCRIPTION (provided by applicant): The AKT signaling pathway is important for the survival of multiple myeloma cells (MM)...
  84. Control of lung permeability by oxidized phospholipids
    Konstantin G Birukov; Fiscal Year: 2007
    ..barrier integrity in the injured lung by triggering intracellular signaling pathways mediated by small GTPases Rac and Cdc42 and their downstream cytoskeletal, FA and AJ protein targets...
  85. Neurogenesis in the Prostate Cancer Microenvironment
    Gustavo Ayala; Fiscal Year: 2008
    ..ABSTRACT NOT PROVIDED Public Health Relevance: This Public Health Relevance is not available...
  86. Mechanisms of Prostacyclin-Mediated Lung Endothelial Barrier Protection
    Konstantin G Birukov; Fiscal Year: 2008
    ..Our published data have shown a reciprocal role for small GTPases Rac and Rho in regulation of endothelial permeability via specific remodeling of actin cytoskeleton and cell contacts...
  87. Chemokines, nitric oxide, and myocardial depression
    Bysani Chandrasekar; Fiscal Year: 2005
    ..The results from these studies will further our knowledge of chemokine biology and lead to new therapeutic strategies to prevent or treat myocardial dysfunction in ischemia-reperfusion injury...
  88. Modulation of Neutrophil Apoptosis by Akt-Hsp27 Signalosome
    Madhavi J Rane; Fiscal Year: 2008
    ..This abstract is not available. Public Health Relevance: This Public Health Relevance is not available...
  89. MMPS IN HIV DEMENTIA: EFFECTS ON BBB STRUCTURE/FUNCTION
    KATHERINE E CONANT; Fiscal Year: 2003
    ..Also, the proposed studies will use both primary brain-derived HIV isolates, which differ from laboratory adapted strains, and normal human cells, which also differ significantly from those of rodent or tumor origin...
  90. Role of Hsp27 in regulation of Neutrophil Apoptosis
    Madhavi J Rane; Fiscal Year: 2006
    ..The broad long-term objective of our laboratory is to elucidate the role of Akt in regulating PMN functions...

Publications124 found, 100 most recent shown here

  1. Okadaic acid inhibits insulin-induced glucose transport in fetal brown adipocytes in an Akt-independent and protein kinase C zeta-dependent manner
    A M Valverde
    Departamento de Bioquímica y Biología Molecular, Centro Mixto CSIC/UCM, Facultad de Farmacia, Universidad Complutense, 28040, Madrid, Spain
    FEBS Lett 472:153-8
    ..However, activation of Akt/PKB or p70 S6 kinase (p70(s6k)) by insulin remained unaltered...
  2. Defective insulin-induced GLUT4 translocation in skeletal muscle of high fat-fed rats is associated with alterations in both Akt/protein kinase B and atypical protein kinase C (zeta/lambda) activities
    F Tremblay
    Department of Physiology, Laval University Hospital Research Center, Quebec, Canada
    Diabetes 50:1901-10
    ..Impairment of PI 3-kinase function was associated with defective Akt/protein kinase B kinase activity (-40%, P < 0...
  3. Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes
    R Hernandez
    Departamento de Bioquimica y Biologia Molecular II, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    FEBS Lett 494:225-31
    ..activity, (2) Akt phosphorylation on the regulatory residue Ser473 but not on Thr308, and (3) mobility shift in Akt1 and Akt2. However, ML-9 did not affect insulin-stimulated PI3-kinase nor PKCzeta activities...
  4. The small GTP-binding protein rho activates c-Jun N-terminal kinases/stress-activated protein kinases in human kidney 293T cells. Evidence for a Pak-independent signaling pathway
    H Teramoto
    Laboratory of Cellular Development and Oncology, NIDR, National Institutes of Health, Bethesda, Maryland 20892 4330, USA
    J Biol Chem 271:25731-4
    ..that in the human kidney epithelial cell line, 293T, Cdc42 and all Rho proteins, RhoA, RhoB, and RhoC, but not Rac or Ras can induce activation of JNK...
  5. Role of three FKHR phosphorylation sites in insulin inhibition of FKHR action in hepatocytes
    A O Scheimann
    Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA
    Horm Metab Res 33:631-8
    ..cells, three serine/threonine moieties in FKHR family members are phosphorylated after insulin treatment or protein kinase B/Akt (PKB) transfection, and each of the three phosphorylated PKB sites contributes to insulin- or PKB-..
  6. Selective small-molecule inhibitors of glycogen synthase kinase-3 activity protect primary neurones from death
    D A Cross
    Neurology Centre of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Harlow, Essex, UK
    J Neurochem 77:94-102
    The phosphatidylinositol 3-kinase (PI 3-kinase)/protein kinase B (PKB; also known as Akt) signalling pathway is recognized as playing a central role in the survival of diverse cell types...
  7. Inhibition of GSK-3 selectively reduces glucose-6-phosphatase and phosphatase and phosphoenolypyruvate carboxykinase gene expression
    P A Lochhead
    Division of Cell Signalling, School of Life Sciences, University of Dundee, UK
    Diabetes 50:937-46
    ..Glycogen synthase kinase-3 (GSK-3) is inhibited following activation of PI 3-kinase and protein kinase B. In hepatoma cells, we find that selectively reducing GSK-3 activity strongly reduces the expression of both ..
  8. Insulin activates protein kinases C-zeta and C-lambda by an autophosphorylation-dependent mechanism and stimulates their translocation to GLUT4 vesicles and other membrane fractions in rat adipocytes
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:25308-16
    ..kinase-1 (PDK-1) is required for phosphorylation of activation loops of PKC-zeta and protein kinase B, we compared their activation...
  9. Protein kinase C isoforms involved in the transcriptional activation of cyclin D1 by transforming Ha-Ras
    S Kampfer
    Institute of Medical Chemistry and Biochemistry, University of Innsbruck, A-6020 Innsbruck, Austria
    J Biol Chem 276:42834-42
    ..The present investigations revealed that cyclin D1 induction by transforming Ha-Ras is MEK- and Rac-dependent and requires the PKC isotypes epsilon, lambda, and zeta, but not cPKC-alpha...
  10. Okadaic acid activates atypical protein kinase C (zeta/lambda) in rat and 3T3/L1 adipocytes. An apparent requirement for activation of Glut4 translocation and glucose transport
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:14074-8
    ..forms of PKC-zeta and PKC-lambda but not by a double mutant (T308A, S473A), activation-resistant form of protein kinase B. Our findings suggest that, as with insulin, PI 3-kinase-dependent atypical PKCs, zeta and lambda, are ..
  11. Modulation by nicotinamide adenine dinucleotide of sympathetic and sensory-motor neurotransmission via P1-purinoceptors in the rat mesenteric arterial bed
    V Ralevic
    Department of Anatomy and Developmental Biology, University College London
    Br J Pharmacol 114:1541-8
    ..antagonist, 8-para-sulphophenyltheophylline (8-pST; 3 microM) inhibited vasodilator responses to NAD (pKB of 6.61 +/- 0.21, n = 7) and adenosine (pKB of 5.78 +/- 0...
  12. Dual regulation of glycogen synthase kinase-3beta by the alpha1A-adrenergic receptor
    L M Ballou
    Department of Pharmacology, University of Texas Health Science Center, San Antonio, Texas 78284, USA
    J Biol Chem 276:40910-6
    ..Insulin-induced GSK-3beta phosphorylation is mediated by the phosphatidylinositol 3-kinase/Akt signaling pathway. PE treatment does not activate phosphatidylinositol 3-kinase or Akt (Ballou, L. M., Cross, M. E...
  13. Stem cell factor/c-kit up-regulates cyclin D3 and promotes cell cycle progression via the phosphoinositide 3-kinase/p70 S6 kinase pathway in spermatogonia
    L X Feng
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC. 20007, USA
    J Biol Chem 275:25572-6
    ..Furthermore, AKT, but not protein kinase C-zeta, is used by SCF/c-kit/PI3-K to activate p70S6K...
  14. 5-Hydroxytryptamine receptor-mediated phosphoinositide hydrolysis in canine cultured tracheal smooth muscle cells
    C M Yang
    Department of Pharmacology, Chang Gung Medical College, Kwei San, Tao Yuan, Taiwan
    Br J Pharmacol 111:777-86
    ..10 and 100 nM), 5-HT2 receptor antagonists, were equipotent in blocking the 5-HT-induced IPs accumulation with pKB values of 8.46 and 8.21, respectively...
  15. Bradykinin-stimulated phosphoinositide metabolism in cultured canine tracheal smooth muscle cells
    C M Yang
    Department of Pharmacology, Chang Gung Medical College, Kwei San, Tao Yuan, Taiwan
    Br J Pharmacol 111:21-8
    ..Thi5,8, D-Phe7]-BK, B2 receptor antagonists, were equipotent in blocking the BK-induced IPs accumulation with pKB = 7.1 and 7.3, respectively. 3...
  16. Requirement of protein kinase C zeta for stimulation of protein synthesis by insulin
    R Mendez
    Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130, USA
    Mol Cell Biol 17:5184-92
    ..synthesis but not activation of p70S6K indicates that PKC zeta activation does not involve the proto-oncogene Akt, which is also activated by PI3K...
  17. Free fatty acid-induced inhibition of glucose and insulin-like growth factor I-induced deoxyribonucleic acid synthesis in the pancreatic beta-cell line INS-1
    S P Cousin
    Pacific Northwest Research Institute, and Department of Pharmacology, University of Washington, Seattle, Washington 98122, USA
    Endocrinology 142:229-40
    ..However, glucose-/IGF-I-induced activation of protein kinase B (PKB) was significantly inhibited, and protein kinase Czeta was chronically activated by FFA...
  18. Protein kinase Czeta activation mediates glucagon-like peptide-1-induced pancreatic beta-cell proliferation
    J Buteau
    Molecular Nutrition Unit, Department of Nutrition, University of Montreal, the Centre de Recherche du CHUM and Institut du Cancer, Montreal, Quebec, Canada
    Diabetes 50:2237-43
    ..GLP-1 increased extracellular signal-related kinase 1/2, p38 mitogen-activated protein kinase (MAPK), and protein kinase B activities nonadditively with glucose in pancreatic beta(INS 832/13) cells...
  19. Inhibition of insulin-induced glucose uptake by atypical protein kinase C isotype-specific interacting protein in 3T3-L1 adipocytes
    K Kotani
    Second Department of Internal Medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan
    J Biol Chem 275:26390-5
    ..PKClambda but not PKCzeta, ASIP was co-immunoprecipitated with endogenous PKClambda but not with PKCepsilon or with Akt. The subcellular localization of PKClambda was also altered in cells overexpressing ASIP...
  20. Uncoupling IL-2 signals that regulate T cell proliferation, survival, and Fas-mediated activation-induced cell death
    L Van Parijs
    Department of Biology, California Institute of Technology, Pasadena 91125, USA
    Immunity 11:281-8
    ..T cell survival was dependent on a receptor region that activated Akt and the expression of Bcl-2...
  21. The pleckstrin homology domain of RAC protein kinase associates with the regulatory domain of protein kinase C zeta
    H Konishi
    Biosignal Research Center, Kobe University, Japan
    Biochem Biophys Res Commun 205:1770-5
    Binding proteins to the pleckstrin homology domain of RAC protein kinase were screened by using glutathione S-transferase fusion protein system...
  22. Inhibition by SR 140333 of NK1 tachykinin receptor-evoked, nitric oxide-dependent vasodilatation in the hamster cheek pouch microvasculature in vivo
    J M Hall
    Biomedical Sciences Division, King s College London
    Br J Pharmacol 113:522-6
    ..of preconstricted arterioles per se, but potently inhibited dilator responses to substance P methyl ester (apparent pKB 9.9 +/- 0.2; n = 5 hamsters, n = 10 estimates)...
  23. Bradykinin B2 receptors and coupling mechanisms in the smooth muscle of the guinea-pig taenia caeci
    J L Field
    Biomedical Sciences Division, King s College London, Chelsea, London
    Br J Pharmacol 113:607-13
    ..30-300 nM), inhibited both the bradykinin-induced relaxation and contraction with a similar affinity (apparent pKB estimates of 8.5 +/- 0.1 and 8.4 +/- 0.1 respectively). 3...
  24. The interaction of the NK1 receptor antagonist CP-96,345 with L-type calcium channels and its functional consequences
    S Guard
    Parke Davis Neuroscience Research Centre, Addenbrookes Hospital Site, Cambridge
    Br J Pharmacol 110:385-91
    ..selective NK1 agonists [Sar9, Met(O2)11]SP and substance P-methyl ester that was apparently competitive in nature (pKB 7.0-7.5), while in guinea-pig ileum the antagonism was not surmountable. 3...
  25. Bradykinin B1 receptors in the rabbit urinary bladder: induction of responses, smooth muscle contraction, and phosphatidylinositol hydrolysis
    S K Butt
    Biomedical Sciences Division, King s College London, Chelsea
    Br J Pharmacol 114:612-7
    ..antagonists [Leu8,des-Arg9]-BK and D-Arg-[Hyp3,Thi5,D-Tic7,Oic8,des-Arg9]-BK ([des-Arg10]-Hoe140) (pKB estimates were 6.1 +/- 0.1 and 7.1 +/- 0...
  26. The pharmacology of RS-15385-197, a potent and selective alpha 2-adrenoceptor antagonist
    C M Brown
    Syntex Research Centre, Research Park, Riccarton, Edinburgh
    Br J Pharmacol 108:516-25
    ..An apparent pKB of 5.9 was obtained against cirazoline-induced contractions in DSV, whilst a pA2 of 6...
  27. Pharmacological profile of GR117289 in vitro: a novel, potent and specific non-peptide angiotensin AT1 receptor antagonist
    M J Robertson
    Department of Cardiovascular and Respiratory Pharmacology, Glaxo Group Research Ltd, Ware, Herts
    Br J Pharmacol 107:1173-80
    ..A pKB of 9.8 +/- 0.1 was calculated for GR117289 after 3 h incubation...
  28. 5-Hydroxytryptamine receptors that facilitate excitatory neuromuscular transmission in the guinea-pig isolated detrusor muscle
    E Messori
    Department of Internal Medicine and Therapeutics, University of Pavia, Italy
    Br J Pharmacol 115:677-83
    ..effect on the high potency phase, but caused a rightward parallel shift of the low potency phase of the 5-HT curve (pKB = 7.3). Granisetron(0.3 microM) antagonized with comparable affinity (pKB = 7...
  29. Dysfunction of muscarinic M2 receptors after the early allergic reaction: possible contribution to bronchial hyperresponsiveness in allergic guinea-pigs
    R E Ten Berge
    Department of Medicinal Chemistry and Molecular Pharmacology, University Centre for Pharmacy, Groningen, The Netherlands
    Br J Pharmacol 114:881-7
    ..in cardiac muscarinic M2 receptor function, since in left atria pD2 (-log EC50) and Emax values of pilocarpine and pKB values of AQ-RA 741, a selective M2 receptor antagonist, were not significantly different from controls (..
  30. FPL 66096: a novel, highly potent and selective antagonist at human platelet P2T-purinoceptors
    R G Humphries
    Department of Pharmacology, Fisons plc, Loughborough, Leics
    Br J Pharmacol 113:1057-63
    ..Logistic fitting of E/[A] data indicated that the effect of FPL 66096 was consistent with simple competition with a pKB value of 8.66...
  31. Heterogeneity of muscarinic receptors in lamb isolated coronary resistance arteries
    U Simonsen
    Departamento de Fisiología, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
    Br J Pharmacol 109:998-1007
    ..The pKB values for the antagonists from plots constrained to unity in endothelium-intact segments were: atropine (9...
  32. The interaction of RS 25259-197, a potent and selective antagonist, with 5-HT3 receptors, in vitro
    E H Wong
    Institute of Pharmacology, Palo Alto, California 94303, USA
    Br J Pharmacol 114:851-9
    ..RS 25259-197 antagonized contractile responses to 5-HT in an unsurmountable fashion and the apparent affinity (pKB), estimated at 10 nM, was 8.8 +/- 0.2. In this tissue, the -log KB values for the other three enantiomers were 6...
  33. The relationship between density of alpha-adrenoceptor binding sites and contractile responses in several porcine isolated blood vessels
    I K Wright
    Department of Physiology and Pharmacology, Medical School, Queen s Medical Centre, Nottingham
    Br J Pharmacol 114:678-88
    ..60 +/- 0.15). Further, rauwolscine (1-10 microM) antagonized NA-induced contractions with an apparent pKB of 6.09 +/- 0.11 (n = 6), indicating an action at alpha 1-adrenoceptors...
  34. Differential effects of P2-purinoceptor antagonists on phospholipase C- and adenylyl cyclase-coupled P2Y-purinoceptors
    J L Boyer
    Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27599
    Br J Pharmacol 113:614-20
    ..cells, suramin and reactive blue 2 competitively antagonized the inhibitory effect of 2MeSATP on adenylyl cyclase (pKB = 5.4 +/- 0.2 and 7.6 +/- 0.1, respectively), whereas PPADS at concentrations up to 100 microM had no effect. 4...
  35. Discriminative stimulus effects of opioids in pigeons trained to discriminate fentanyl, bremazocine and water: evidence of pharmacological selectivity
    M J Picker
    Department of Psychology, University of North Carolina at Chapel Hill 27599 3270, USA
    Behav Pharmacol 8:160-73
    ..The apparent pkB values obtained for naloxone as an antagonist of the stimulus effects of fentanyl were higher than those obtained ..
  36. 5-Hydroxytryptamine (5-HT) mediates potent relaxation in the sheep isolated pulmonary vein via activation of 5-HT4 receptors
    T M Cocks
    Baker Medical Research Institute, Prahran, Victoria, Australia
    Br J Pharmacol 107:591-6
    ..whilst that toa-methyl-5-HT and BIMU 8 was unaffected by methiothepin (10 nM) but blocked by ICS 205-930(estimated pKB values of 6.4 and 6.9 respectively)...
  37. Investigation of the 5-hydroxytryptamine receptor mediating the 'maintained' short-circuit current response in guinea-pig ileal mucosa
    C M Scott
    Department of Gastrointestinal Pharmacology, Glaxo Group Research Ltd, Ware, Herts
    Br J Pharmacol 106:877-82
    ..Metoclopramide and cisapride inhibited the SCC responses to 5-HT with apparent pKB values of 4.8 and 7.0 respectively. 5...
  38. Involvement of multiple receptors in the biological effects of calcitonin gene-related peptide and amylin in rat and guinea-pig preparations
    S Giuliani
    Pharmacology Department, A Menarini Pharmaceuticals, Florence, Italy
    Br J Pharmacol 107:510-4
    ..Apparent pKB values calculated on the basis of the displacement produced by 1 microM human alpha CGRP(8-37) indicated an agonist-..
  39. Characterization of the 5-HT4 receptor mediating tachycardia in piglet isolated right atrium
    A D Medhurst
    SmithKline Beecham Pharmaceuticals, The Pinnacles, Harlow, Essex
    Br J Pharmacol 110:1023-30
    ..We estimated a pKB of 7.9 for BIMU 1 antagonism of 5-HT-induced tachycardia. DAU 6215 (N-endo-8-methyl-8-azabicyclo[3.2...
  40. Functional evidence equating the pharmacologically-defined alpha 1A- and cloned alpha 1C-adrenoceptor: studies in the isolated perfused kidney of rat
    D R Blue
    Syntex Research, Palo Alto, CA 94304, USA
    Br J Pharmacol 115:283-94
    ..competitively with noradrenaline at the alpha 1-adrenoceptor for which 5-methyl-urapidil exhibits high affinity (pKB value): WB 4101 (10.3) > prazosin (9.5) approximately HV 723 (9.3) approximately 5-methyl-urapidil (9...
  41. Pharmacological profile of valsartan: a potent, orally active, nonpeptide antagonist of the angiotensin II AT1-receptor subtype
    L Criscione
    Cardiovascular Research Department, Ciba Geigy Limited, Basel, Switzerland
    Br J Pharmacol 110:761-71
    ..After 3 h incubation an apparent pKb value of 9.26 was calculated...
  42. Simvastatin induces regression of cardiac hypertrophy and fibrosis and improves cardiac function in a transgenic rabbit model of human hypertrophic cardiomyopathy
    R Patel
    Section of Cardiology, Department of Medicine, The DeBakey Heart Center, The Methodist Hospital and Baylor College of Medicine, Houston, Texas, USA
    Circulation 104:317-24
    ..Levels of activated and total p38, Jun N-terminal kinase, p70S6 kinase, Ras, Rac, and RhoA and the membrane association of Ras, RhoA, and Rac1 were unchanged...
  43. A pharmacological study of NK1 and NK2 tachykinin receptor characteristics in the rat isolated urinary bladder
    J M Hall
    Pharmacology Group, King s College London, London
    Br J Pharmacol 107:777-84
    ..Markedly different pKB estimates were obtained in the rat bladder (6.38) and rat ileum (6.56) compared to the guinea-pig ileum (7.42)...
  44. The use of antagonists to characterize the receptors mediating depolarization of the rat isolated vagus nerve by alpha, beta-methylene adenosine 5'-triphosphate
    D J Trezise
    Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge
    Br J Pharmacol 112:282-8
    ..concentration-effect curve to alpha, beta-methylene ATP was shifted to the right in a parallel manner, yielding a pKB estimate of 4.96. 4...
  45. Comparison of tachykinin NK1 and NK2 receptors in the circular muscle of the guinea-pig ileum and proximal colon
    C A Maggi
    Pharmacology and Chemistry Department, A Menarini Pharmaceuticals, Florence, Italy
    Br J Pharmacol 112:150-60
    ..MEN 10,573 antagonized the response to [Sar9]SP sulphone and septide in both preparations,pKB values against septide being intermediate, and significantly different from, those measured against[Beta Ala 8]NKA(..
  46. Differential scanning calorimetry and X-ray diffraction studies of the thermotropic phase behavior of the diastereomeric di-tetradecyl-beta-D-galactosyl glycerols and their mixture
    D A Mannock
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biophys J 66:734-40
    ..to an Lc phase at a rate which is strongly dependent on the chirality of the glycerol backbone (1,2-sn > 1,2-rac > 2,3-sn)...
  47. Pharmacological properties of the cloned alpha 1A/D-adrenoceptor subtype are consistent with the alpha 1A-adrenoceptor characterized in rat cerebral cortex and vas deferens
    B A Kenny
    Department of Discovery Biology, Pfizer Central Research, Sandwich, Kent
    Br J Pharmacol 111:1003-8
    ..23 +/- 0.28), WB 4101 (pA2 9.58 +/- 0.12), phentolamine (pKB 7.90 +/- 0.16), benoxathian (pKB 9.21 +/- 0.21)and 5-methyl-urapadil (pKB 8.51 +/-0...
  48. Pharmacological characterization of the contractile responses to angiotensin analogues in guinea-pig isolated longitudinal muscle of small intestine
    A B Hawcock
    Neuropharmacology Department, Glaxo Group Research Ltd, Ware, Hertfordshire
    Br J Pharmacol 108:1150-5
    ..displacement of the concentration-response curve to angiotensin II and [Lys2]angiotensin II, with an estimated pKB of 8.56(8.42-8.68) and 9.18 (8.63-9.50) respectively...
  49. Evaluation of ICI D7114, a putative stimulant of brown adipocytes, on histamine-contracted guinea-pig ileum
    J W Growcott
    VIM Research Department, Zeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire
    Br J Pharmacol 109:1212-8
    ..to ICI D7114 and BRL37344 were antagonized by single concentrations of alprenolol (1 microM) with apparent pKB values of 6.53 and 6.57 respectively...
  50. The interaction of antidepressant drugs with central and peripheral (enteric) 5-HT3 and 5-HT4 receptors
    A Lucchelli
    Institute of Pharmacology, School of Pharmacy, University of Pavia, Italy
    Br J Pharmacol 114:1017-25
    ..1 microM) was ineffective on the high-potency phase, but caused a rightward shift of the low-potency phase (pKB: 6.1 +/- 0.01). 5. Responses to 2-methyl-5-HT were inhibited by 1 microM fluoxetine (pKB: 5.4 +/- 0.02)...
  51. Evidence for a capsaicin-sensitive, tachykinin-mediated, component in the NANC contraction of the rat urinary bladder to nerve stimulation
    S Meini
    Pharmacology Department, A Menarini Pharmaceuticals, Florence, Italy
    Br J Pharmacol 112:1123-31
    ..shift in the concentration-response curve tothe NKI receptor agonist, [Sar9]substance P sulphone (apparent pKB 8.97 +/- 0...
  52. Pharmacological characterization and anatomical localization of prejunctional beta-adrenoceptors in the rat kidney
    P P Lakhlani
    Department of Pharmacology, University of Houston, TX 77204 5515
    Br J Pharmacol 111:1296-308
    ..beta 2-adrenoceptor agonist, was inhibited by ICI 118,551, the selective beta 2-adrenoceptor antagonist, with pKb values of 9.20 and 9.35, respectively at renal prejunctional beta-adrenoceptors...
  53. Heterogeneity of alpha 1-adrenoceptor subtypes involved in adrenergic contractions of dog blood vessels
    Y Kohno
    Department of Otorhinolaryngology, Fukui Medical School, Japan
    Br J Pharmacol 112:1167-73
    ..However, there was considerable difference between the vessels with regard to the pKB values for all the antagonists...
  54. Endothelin receptor subtypes in human and guinea-pig pulmonary tissues
    D W Hay
    Department of Inflammation and Respiratory Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406
    Br J Pharmacol 110:1175-83
    ..and selective ETA receptor antagonist, potently antagonized ET-1-induced contraction in human pulmonary artery (pKB = 6.8 with 1 microM BQ-123, n = 7) but had no effect in human bronchus (n = 6). 3. Sarafotoxin S6c (0.1 nM-0...
  55. Involvement of B2 receptors in the bradykinin-induced relaxation of guinea-pig isolated trachea
    A da Silva
    Laboratoire de Neuroimmunopharmacologie, INSERM U405, Université Louis Pasteur Strasbourg I, Faculté de Pharmacie, Illkirch, France
    Br J Pharmacol 114:103-8
    ..2 +/- 0.4, whereas NPC 17761 (0.3 to 1 microM) competitively antagonized bradykinin-induced relaxation with a pKB = 7.3 +/- 0.2. The Schild regression slope did not differ from unity, 0.96 +/- 0.20, P<0.05.4...
  56. Characterization of the 5-hydroxytryptamine receptor type involved in inhibition of spontaneous activity of human isolated colonic circular muscle
    F S Tam
    Clinical Pharmacology Group, Faculty of Medicine, University of Southampton
    Br J Pharmacol 113:143-50
    ..5-HT and 5-methoxytryptamine were competitively antagonized by a weak 5-HT4 receptor antagonist, tropisetron, with pKB values of approximately 6...
  57. Examination of the role of inhibition of cyclic AMP in alpha 2-adrenoceptor mediated contractions of the porcine isolated palmar lateral vein
    I K Wright
    Department of Physiology and Pharmacology, Medical School, Queen s Medical Centre, Nottingham
    Br J Pharmacol 114:157-65
    ..The estimated pKB value for rauwolscine (8.28 +/- 0.19, n = 10) is consistent with inhibition of alpha 2-adrenoceptors...
  58. Characterization of somatostatin receptors in guinea-pig isolated ileum, vas deferens and right atrium
    W Feniuk
    Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge
    Br J Pharmacol 110:1156-64
    ..atrium angiopeptin had low intrinsic activity and antagonized the negative inotropic action of both SS14 and SS28 (pKB values of 7.4 and 7.2, respectively). CGP 23996 was 2-7 times weaker than SS14 in guinea-pig vas deferens and atria...
  59. In vitro and in vivo characterization of NK3 receptors in the rabbit eye by use of selective non-peptide NK3 receptor antagonists
    A D Medhurst
    Department of Neurosciences Research, SmithKline Beecham Pharmaceuticals, Harlow, Essex
    Br J Pharmacol 122:469-76
    ..3 and 3 microM apparent pKB=7.4+/-0.06, n=6). 4...
  60. Evidence for the presence of both pre- and postjunctional P2-purinoceptor subtypes in human isolated urinary bladder
    S Palea
    Department of Pharmacology, Glaxo Research Laboratories, Verona, Italy
    Br J Pharmacol 114:35-40
    ..At 10% of KCl response the Schild plot slope was 0.98 and the estimated pKB was 5...
  61. Breast cancer-associated fibroblasts confer AKT1-mediated epigenetic silencing of Cystatin M in epithelial cells
    Huey Jen L Lin
    Division of Medical Technology, School of Allied Medical Professions, Human Cancer Genetics Program, The Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 68:10257-66
    ..Promoter hypermethylation of CST6 is associated with aberrant AKT1 activation in epithelial cells, as well as the disabled INNP4B regulator resulting from the suppression by CAFs...
  62. TNF-alpha impairs the S-G2/M cell cycle checkpoint and cyclobutane pyrimidine dimer repair in premalignant skin cells: role of the PI3K-Akt pathway
    Annesofie Faurschou
    Department of Dermatology, University of Copenhagen, Bispebjerg Hospital, Copenhagen, Denmark
    J Invest Dermatol 128:2069-77
    ..in normal keratinocytes and the transformed keratinocyte cell lines, HaCaT and A431, TNF-alpha stimulates protein kinase B/Akt, which results in activation of the survival complex mTORC1 (mammalian target of rapamycin complex 1) and ..
  63. Casein Kinase I epsilon positively regulates the Akt pathway in breast cancer cell lines
    Cristina Modak
    Department of Developmental and Cell Biology, University of California Irvine, Mc Gaugh Hall Room 4340, Irvine, CA 92697, USA
    Biochem Biophys Res Commun 368:801-7
    The Akt pathway is very important in both development and cancer...
  64. Early phosphatidylinositol 3-kinase/Akt pathway activation limits poliovirus-induced JNK-mediated cell death
    Arnaud Autret
    Biologie des Virus Entériques, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Virol 82:3796-802
    ..We showed here that PV simultaneously activates the phosphatidylinositol 3-kinase (PI3K)/Akt survival signaling pathway in these cells, limiting the extent of JNK activation and thereby cell death...
  65. Hypoxia inhibits the SDF-1-dependent migration of human leukemic cell line HL-60 via blocking of Akt activation
    Young Jin Seo
    Laboratory of Immunology, School of Biological Sciences, Seoul National University, Seoul 151 747, Republic of Korea
    Biochem Biophys Res Commun 364:388-94
    ..Furthermore, we showed that migration toward SDF-1 was reduced by inactivation of either serine/threonine kinase Akt or extracellular signal regulated kinase Erk, which was confirmed by selective pathway inhibitor LY294002 and ..
  66. Identification of functional domains in AKT responsible for distinct roles of AKT isoforms in pressure-stimulated cancer cell adhesion
    Shouye Wang
    Surgical Service, John D Dingell VA Medical Center, Wayne State University, Detroit, MI 48201, USA
    Exp Cell Res 314:286-96
    ..Reducing AKT1, but not AKT2, ablates the increase in cancer cell adhesion associated with 15 mm Hg increased extracellular ..
  67. Attenuation of apoptosis by chromogranin A-induced Akt and survivin pathways in prostate cancer cells
    Junyang Gong
    Weill Medical College of Cornell University, Department of Urology, Room E 300, Box 23, 1300 York Avenue, New York, New York 10021, USA
    Endocrinology 148:4489-99
    ..Survivin expression is believed to be regulated by PI3K/Akt pathway...
  68. Caspase-3-mediated cleavage of Akt: involvement of non-consensus sites and influence of phosphorylation
    Arezu Jahani Asl
    Department of Obstetrics and Gynaecology, University of Ottawa, Ottawa, Canada
    FEBS Lett 581:2883-8
    Here, we show for the first time that Akt1 is cleaved in vitro at the caspase-3 consensus site DQDD(456) downward arrow SM...
  69. Parathyroid hormone-related protein induces cell survival in human renal cell carcinoma through the PI3K Akt pathway: evidence for a critical role for integrin-linked kinase and nuclear factor kappa B
    Abdelali Agouni
    INSERM U727, Section of Renal Pharmacology and Physiopathology, School of Medicine, University Louis Pasteur, Strasbourg 67085, France
    Carcinogenesis 28:1893-901
    ..Using specific inhibitors of phosphoinositide 3-kinase (PI3K) and depletion of Akt kinase by RNA interference, we established that PTHrP is one of the main factor involved in the constitutive ..
  70. The Par6alpha/aPKC complex regulates Akt1 activity by phosphorylating Thr34 in the PH-domain
    P Weyrich
    Department of Internal Medicine IV, University of Tübingen, Otfried Müller Str 10, D 72076 Tübingen, Germany
    Mol Cell Endocrinol 268:30-6
    ..better insulin sensitivity, whereas overexpression of Par6alpha in C2C12 myoblasts inhibits insulin-induced protein kinase B/Akt1 activation and glycogen synthesis...
  71. Genetic alterations and their relationship in the phosphatidylinositol 3-kinase/Akt pathway in thyroid cancer
    Peng Hou
    Division of Endocrinology and Metabolism, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Clin Cancer Res 13:1161-70
    ..the overall occurrence and relationship of genetic alterations in the phosphatidylinositol 3-kinase (PI3K)/Akt pathway in thyroid tumors and explore the scope of this pathway as a therapeutic target for thyroid cancer...
  72. Ginsenoside-Rg1 induces vascular endothelial growth factor expression through the glucocorticoid receptor-related phosphatidylinositol 3-kinase/Akt and beta-catenin/T-cell factor-dependent pathway in human endothelial cells
    Kar Wah Leung
    Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
    J Biol Chem 281:36280-8
    ..vein endothelial cells, and importantly this induction was mediated through a phosphatidylinositol 3-kinase (PI3K)/Akt and beta-catenin/T-cell factor-dependent pathway via the GR...
  73. Deactivation of phosphatidylinositol 3,4,5-trisphosphate/Akt signaling mediates neutrophil spontaneous death
    Daocheng Zhu
    Department of Pathology, Joint Program in Transfusion Medicine, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:14836-41
    ..b>PKB/Akt possesses prosurvival and antiapoptotic activities in a variety of cells...
  74. Ultraviolet B-induced matrix metalloproteinase-1 and -3 secretions are mediated via PTEN/Akt pathway in human dermal fibroblasts
    Jang Hee Oh
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, South Korea
    J Cell Physiol 209:775-85
    ..We investigated whether phosphatase and tensin homolog (PTEN)/Akt pathway is involved in secretions of MMP-1 and -3 in human dermal fibroblasts...
  75. Protein kinase C epsilon confers resistance of MCF-7 cells to TRAIL by Akt-dependent activation of Hdm2 and downregulation of p53
    E Shankar
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Oncogene 27:3957-66
    ..We have previously shown that PKC epsilon acts upstream of Akt to inhibit receptor-initiated cell death...
  76. Transforming growth factor beta induces apoptosis through repressing the phosphoinositide 3-kinase/AKT/survivin pathway in colon cancer cells
    Jing Wang
    University of Nebraska Medical Center, Eppley Institute for Research in Cancer and Allied Diseases, Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Cancer Res 68:3152-60
    ..in greater endogenous cell survival in response to GFDS due to activation of the phosphoinositide 3-kinase (PI3K)/Akt/survivin pathway...
  77. Cancer cell-derived clusterin modulates the phosphatidylinositol 3'-kinase-Akt pathway through attenuation of insulin-like growth factor 1 during serum deprivation
    Hakryul Jo
    Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Mol Cell Biol 28:4285-99
    ..Here, we describe a cancer cell-derived regulatory system that modulates the phosphatidylinositol 3'-kinase (PI3K)-Akt pathway under serum deprivation stress...
  78. Akt regulates vitamin D3-induced leukemia cell functional differentiation via Raf/MEK/ERK MAPK signaling
    Jianrong Wang
    Department of Molecular Biology and Genetics, Cornell University, New York 14853, USA
    Eur J Cell Biol 88:103-15
    ..Here, we show that vitamin D3 induced functional differentiation by Akt through Raf/MEK/ERK MAPK signaling...
  79. RGS13 controls g protein-coupled receptor-evoked responses of human mast cells
    Geetanjali Bansal
    Molecular Signal Transduction Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 181:7882-90
    ..b>Akt phosphorylation, chemotaxis, and cytokine (IL-8) secretion induced by CXCL12 were also greater in short hairpin ..
  80. A pilot surrogate endpoint biomarker study of celecoxib in oral premalignant lesions
    Lori J Wirth
    Department of Adult Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Cancer Prev Res (Phila Pa) 1:339-48
    ..Treatment with celecoxib in subjects with OPLs favorably modulates the primary mediator of cyclooxygenase-2 activity, PGE(2), after 12 weeks...
  81. AKT1 inhibits homologous recombination by inducing cytoplasmic retention of BRCA1 and RAD51
    Isabelle Plo
    Unité Mixte de Recherche Commissariat à l Energie Atomique CEA Centre National de la Recherche Scientifique CNRS 217, Fontenay aux Roses, 92265, France
    Cancer Res 68:9404-12
    b>AKT1 is frequently up-regulated in sporadic breast cancer, whereas BRCA1 is frequently mutated in familial breast cancer...
  82. beta-arrestin2 inhibits opioid-induced breast cancer cell death through Akt and caspase-8 pathways
    M Zhao
    Department of Internal Medicine, James Quillen College of Medicine, East Tennessee State University, TN, USA
    Neoplasma 56:108-13
    ..by specific RNA interfering (RNAi) and morphine treatment significantly attenuates the levels of phosphorylated Akt compared with inhibition of beta-arrestin2 or morphine treatment alone...
  83. Bortezomib overcomes tumor necrosis factor-related apoptosis-inducing ligand resistance in hepatocellular carcinoma cells in part through the inhibition of the phosphatidylinositol 3-kinase/Akt pathway
    Kuen Feng Chen
    Department of Medical Research, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan
    J Biol Chem 284:11121-33
    ..Third, knocking down Akt1 by small interference RNA also enhanced TRAIL-induced apoptosis in Huh-7 cells...
  84. Activated Akt as an indicator of prognosis in gastric cancer
    Caterina Cinti
    Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, Siena Unit, Siena, Italy
    Virchows Arch 453:449-55
    The immunohistochemical expression of phosphorylated (activated) Akt (pAkt) in 50 advanced gastric carcinomas has been analyzed and the results correlated with age, sex, location in the stomach, histotype, stage, survival, mitotic and ..
  85. Liposome-encapsulated curcumin suppresses growth of head and neck squamous cell carcinoma in vitro and in xenografts through the inhibition of nuclear factor kappaB by an AKT-independent pathway
    Dorothy Wang
    Department of Surgery, VA Greater Los Angeles Healthcare System, and Division of Head and Neck Surgery, David Geffen School of Medicine at University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Clin Cancer Res 14:6228-36
    ..analysis was done to determine the effect of curcumin on the expression of NFkappaB, phospho-IkappaBalpha, phospho-AKT (pAKT), phospho-S6 kinase, cyclin D1, cyclooxygenase-2, matrix metalloproteinase-9, Bcl-2, Bcl-xL, Mcl-1L, and Mcl-..
  86. Overexpression of cFLIPs inhibits oxaliplatin-mediated apoptosis through enhanced XIAP stability and Akt activation in human renal cancer cells
    Shin Kim
    Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University, 194 DongSan Dong Jung Gu, Taegu 700 712, South Korea
    J Cell Biochem 105:971-9
    ..cFLIPs overexpressing cell, which was caused by the increased XIAP protein stability and that the phospho-Akt level was high compared to vector-transfected cell...
  87. Tissue kallikrein promotes neovascularization and improves cardiac function by the Akt-glycogen synthase kinase-3beta pathway
    Yu Yu Yao
    Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210029, People s Republic of China
    Cardiovasc Res 80:354-64
    AIMS: We investigated the role of the Akt-glycogen synthase kinase (GSK)-3beta signalling pathway in mediating the protective effects of tissue kallikrein on myocardial injury by promoting angiogenesis and blood flow in rats after ..
  88. Expression of transketolase-like 1 (TKTL1) and p-Akt correlates with the progression of cervical neoplasia
    Nico Kohrenhagen
    Departments of Obstetrics and Gynecology, University of Wuerzburg, Wuerzburg, Germany
    J Obstet Gynaecol Res 34:293-300
    ..Transketolase-like enzyme 1 (TKTL1) and the oncogene p-Akt have been described to play an important role in glycolysis during tumorigenesis...
  89. Prognostic associations of activated mitogen-activated protein kinase and Akt pathways in glioblastoma
    Christopher E Pelloski
    Department of Radiation Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 12:3935-41
    PURPOSE: Activation of mitogen-activated protein kinase (MAPK) and members of the Akt pathway have been shown to promote cell proliferation, survival, and resistance to radiation...
  90. Germline inactivation of PTEN and dysregulation of the phosphoinositol-3-kinase/Akt pathway cause human Lhermitte-Duclos disease in adults
    Xiao Ping Zhou
    Clinical Cancer Genetics and Human Cancer Genetics Programs, Comprehensive Cancer Center, and the Division of Human Genetics, Department of Internal Medicine, Columbus, OH 43210, USA
    Am J Hum Genet 73:1191-8
    ..that 75% of the LDD samples had complete or partial loss of PTEN expression accompanied by elevated phosphorylated Akt, specifically in the dysplastic gangliocytoma cells...
  91. Increase of AKT/PKB expression correlates with gleason pattern in human prostate cancer
    Yongde Liao
    Research Group Hormones and Signal Transduction, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Int J Cancer 107:676-80
    AKT/PKB is a central signaling molecule related to stimulation of cell proliferation and inhibition of apoptosis. Perturbations of AKT expression and function play an important role in tumor development and progression...
  92. Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1
    Hung Kai Chen
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 113:457-68
    ..The association of ataxin-1 with 14-3-3 is regulated by Akt phosphorylation, and in a Drosophila model of SCA1, both 14-3-3 and Akt modulate neurodegeneration...
  93. Identification of 14-3-3zeta as a protein kinase B/Akt substrate
    David W Powell
    Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, Kentucky 40202 1718, USA
    J Biol Chem 277:21639-42
    b>Protein kinase B/Akt (PKB/Akt) is a member of the ACG kinase family, which also includes protein kinase C, that phosphorylates a number of 14-3-3-binding proteins...
  94. Phosphorylation of Fanconi anemia protein, FANCA, is regulated by Akt kinase
    Tetsuya Otsuki
    Department of Hematology, Center for Molecular Medicine, Jichi Medical School, Yakushiji 3311 1, Minamikawachi, Kawachi, Tochigi, 329 0498, Japan
    Biochem Biophys Res Commun 291:628-34
    ..However, the kinase for FANCA (so-called FANCA-PK) remains to be identified. FANCA has a consensus sequence for Akt kinase near serine 1149 (Ser1149), suggesting that Akt can phosphorylate FANCA...
  95. AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival
    Ying Li
    Department of Molecular Oncology, Genentech, Inc, San Francisco, California 94080, USA
    J Biol Chem 277:11352-61
    ..Here we demonstrate that p21 is directly phosphorylated by AKT/PKB, a survival kinase that is hyperactivated in many late stage tumors...
  96. Protein kinase B phosphorylates AHNAK and regulates its subcellular localization
    J Sussman
    Cancer Research Institute, University of California at San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 154:1019-30
    ..We show that AHNAK is a phosphorylation substrate of protein kinase B (PKB) in vitro and in vivo...