Gene Symbol: AGO2
Description: argonaute 2, RISC catalytic component
Alias: CASC7, EIF2C2, LINC00980, PPD, Q10, protein argonaute-2, PAZ Piwi domain protein, argonaute RISC catalytic component 2, cancer susceptibility candidate 7, cancer susceptibility candidate 7 (non-protein coding), eukaryotic translation initiation factor 2C, 2, long intergenic non-protein coding RNA 980, protein slicer
Species: human
Products:     AGO2

Top Publications

  1. Wang M, Ren D, Guo W, Wang Z, Huang S, Du H, et al. Loss of miR-100 enhances migration, invasion, epithelial-mesenchymal transition and stemness properties in prostate cancer cells through targeting Argonaute 2. Int J Oncol. 2014;45:362-72 pubmed publisher
    ..Argonaute 2 (AGO2) is the core effector protein of the miRNA-induced silencing complex and overexpression of AGO2 might enhance tumor ..
  2. Savas J, Makusky A, Ottosen S, Baillat D, Then F, Krainc D, et al. Huntington's disease protein contributes to RNA-mediated gene silencing through association with Argonaute and P bodies. Proc Natl Acad Sci U S A. 2008;105:10820-5 pubmed publisher
    ..Colocalization studies demonstrated Htt and Ago2 to be present in P bodies, and depletion of Htt showed compromised RNA-mediated gene silencing...
  3. Chang S, Smith I, Glazer C, Hennessey P, Califano J. EIF2C is overexpressed and amplified in head and neck squamous cell carcinoma. ORL J Otorhinolaryngol Relat Spec. 2010;72:337-43 pubmed publisher
    ..Eukaryotic translation initiation factor 2C subunit 2 (EIF2C2) was found to be the most significantly overexpressed gene by mRNA expression array, and corresponded to the most ..
  4. Chu C, Rana T. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol. 2006;4:e210 pubmed
    ..Both RISCs contain Argonaute2 (Ago2), which catalyzes target mRNA cleavage by siRISC and localizes to cytoplasmic mRNA processing bodies (P-bodies)...
  5. Meister G, Landthaler M, Peters L, Chen P, Urlaub H, Luhrmann R, et al. Identification of novel argonaute-associated proteins. Curr Biol. 2005;15:2149-55 pubmed
    ..Our biochemical analysis revealed that Ago2 is present in a pre-miRNA processing complex that is able to transfer the miRNA into a target-mRNA cleaving complex...
  6. Kinch L, Grishin N. The human Ago2 MC region does not contain an eIF4E-like mRNA cap binding motif. Biol Direct. 2009;4:2 pubmed publisher
    ..A recent report by Kiriakidou et al. 1 describes an MC sequence region identified in Ago2 that displays similarity to the cap-binding motif in translation initiation factor 4E (eIF4E)...
  7. Baillat D, Shiekhattar R. Functional dissection of the human TNRC6 (GW182-related) family of proteins. Mol Cell Biol. 2009;29:4144-55 pubmed publisher
    ..In contrast, the C-terminal domain containing the RNA recognition motif plays a critical role in the silencing mediated by the TNRC6B protein. ..
  8. Cheng N, Li Y, Han Z. Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma. Hepatology. 2013;57:1906-18 pubmed publisher
    ..Here we evaluate the effect of Argonaute2 (Ago2), a member of the Ago gene family that plays a role in short interfering RNA-mediated gene silencing, on HCC ..
  9. Kiriakidou M, Tan G, Lamprinaki S, De Planell Saguer M, Nelson P, Mourelatos Z. An mRNA m7G cap binding-like motif within human Ago2 represses translation. Cell. 2007;129:1141-51 pubmed
    ..We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of ..

More Information


  1. Zeng Y, Sankala H, Zhang X, Graves P. Phosphorylation of Argonaute 2 at serine-387 facilitates its localization to processing bodies. Biochem J. 2008;413:429-36 pubmed publisher
    ..To explore potential regulatory mechanisms for Ago proteins, we examined the phosphorylation of human Ago2. We identified serine-387 as the major Ago2 phosphorylation site in vivo...
  2. Höck J, Weinmann L, Ender C, Rüdel S, Kremmer E, Raabe M, et al. Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. EMBO Rep. 2007;8:1052-60 pubmed
    ..Here, we analysed Ago1- and Ago2-containing protein complexes in human cells...
  3. Schirle N, Macrae I. The crystal structure of human Argonaute2. Science. 2012;336:1037-40 pubmed publisher
    ..The 2.3 angstrom resolution crystal structure of human Argonaute2 (Ago2) reveals a bilobed molecule with a central cleft for binding guide and target RNAs...
  4. Rivas F, Tolia N, Song J, Aragon J, Liu J, Hannon G, et al. Purified Argonaute2 and an siRNA form recombinant human RISC. Nat Struct Mol Biol. 2005;12:340-9 pubmed publisher
    ..These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex...
  5. Shen J, Xia W, Khotskaya Y, Huo L, Nakanishi K, Lim S, et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature. 2013;497:383-7 pubmed publisher
    ..of specific tumour-suppressor-like miRNAs in response to hypoxic stress through phosphorylation of argonaute 2 (AGO2) at Tyr 393...
  6. Li L, Yu C, Gao H, Li Y. Argonaute proteins: potential biomarkers for human colon cancer. BMC Cancer. 2010;10:38 pubmed publisher
    ..from colon cancer and 75 specimens from adjacent non-cancer tissue, and assayed eight different proteins (EIF2C1, EIF2C2, EIF2C3, EIF2C4, PIWIL1, PIWIL2, PIWIL3 and PIWIL4) by immunohistochemistry on consecutive formalin-fixed tissue ..
  7. Johnston M, Geoffroy M, Sobala A, Hay R, Hutvagner G. HSP90 protein stabilizes unloaded argonaute complexes and microscopic P-bodies in human cells. Mol Biol Cell. 2010;21:1462-9 pubmed publisher
    ..Our data suggests that HSP90 protein chaperones Argonautes before binding RNA and may facilitate efficient loading of small RNA. ..
  8. Diederichs S, Jung S, Rothenberg S, Smolen G, Mlody B, Haber D. Coexpression of Argonaute-2 enhances RNA interference toward perfect match binding sites. Proc Natl Acad Sci U S A. 2008;105:9284-9 pubmed publisher
    ..Here, we show that ectopic expression of the RISC slicer Argonaute-2 (Ago2, eIF2C2) dramatically enhances RNAi specifically for mRNA targets with perfectly matched binding sites...
  9. Bouttier M, Saumet A, Peter M, Courgnaud V, Schmidt U, Cazevieille C, et al. Retroviral GAG proteins recruit AGO2 on viral RNAs without affecting RNA accumulation and translation. Nucleic Acids Res. 2012;40:775-86 pubmed publisher
    ..of cellular translation, this recognition is thought to tether the RNAi machinery, in particular Argonaute 2 (AGO2) on viral messengers and eventually to modulate virus replication...
  10. Frank F, Sonenberg N, Nagar B. Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2. Nature. 2010;465:818-22 pubmed publisher
    ..Here we report the crystal structure of a MID domain from a eukaryotic AGO protein, human AGO2. The structure, in complex with nucleoside monophosphates (AMP, CMP, GMP, and UMP) mimicking the 5' end of miRNAs, ..
  11. Kandeel M, Kitade Y. In silico molecular docking analysis of the human Argonaute 2 PAZ domain reveals insights into RNA interference. J Comput Aided Mol Des. 2013;27:605-14 pubmed publisher
    ..of small interfering RNA by PAZ in a computational study based on the structure of Drosophila Argonaute 2 (Ago2) PAZ domain. We have now reanalyzed these data within the view of the new available structures from human Argonauts...
  12. Pare J, Tahbaz N, López Orozco J, LaPointe P, Lasko P, Hobman T. Hsp90 regulates the function of argonaute 2 and its recruitment to stress granules and P-bodies. Mol Biol Cell. 2009;20:3273-84 pubmed publisher
    ..Together, our data suggest that Hsp90 is a critical modulator of Argonaute function. Moreover, we propose that Ago2 and PACT form a complex that functions at the level of SGs.
  13. Tahbaz N, Kolb F, Zhang H, Jaronczyk K, Filipowicz W, Hobman T. Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. EMBO Rep. 2004;5:189-94 pubmed
    PAZ PIWI domain (PPD) proteins, together with the RNA cleavage products of Dicer, form ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs)...
  14. Wu C, So J, Davis Dusenbery B, Qi H, Bloch D, Shi Y, et al. Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2. Mol Cell Biol. 2011;31:4760-74 pubmed publisher
    ..I collagen prolyl-4-hydroxylase [C-P4H(I)], which leads to prolyl-hydroxylation and accumulation of Argonaute2 (Ago2), a critical component of the RNA-induced silencing complex (RISC)...
  15. Liu J, Carmell M, Rivas F, Marsden C, Thomson J, Song J, et al. Argonaute2 is the catalytic engine of mammalian RNAi. Science. 2004;305:1437-41 pubmed
    ..Thus, our evidence supports a model in which Argonaute contributes "Slicer" activity to RISC, providing the catalytic engine for RNAi. ..
  16. Robb G, Rana T. RNA helicase A interacts with RISC in human cells and functions in RISC loading. Mol Cell. 2007;26:523-37 pubmed
    ..Minimally, the action of RISC requires the endonucleolytic slicer activity of Argonaute2 (Ago2) directed to RNA targets whose sequences are complementary to RISC-incorporated small RNA...
  17. Li L, Zhu D, Huang L, Zhang J, Bian Z, Chen X, et al. Argonaute 2 complexes selectively protect the circulating microRNAs in cell-secreted microvesicles. PLoS ONE. 2012;7:e46957 pubmed publisher
    ..Here we show that Argonaute 2 (Ago2) complexes and microvesicles (MVs) provide specific and non-specific protection for miRNA in cell-secreted MVs, ..
  18. Jagannath A, Wood M. Localization of double-stranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2. Mol Biol Cell. 2009;20:521-9 pubmed publisher
    ..Key effectors of microRNA (miRNA)-mediated RNA interference (RNAi), such as Argonaute-2 (Ago2), miRNAs, and their cognate mRNAs, are localized to these structures; however, the precise role that P-bodies and ..
  19. Fabian M, Mathonnet G, Sundermeier T, Mathys H, Zipprich J, Svitkin Y, et al. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009;35:868-80 pubmed publisher
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation...
  20. Arroyo J, Chevillet J, Kroh E, Ruf I, Pritchard C, Gibson D, et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A. 2011;108:5003-8 pubmed publisher
    ..Further characterization revealed that Argonaute2 (Ago2), the key effector protein of miRNA-mediated silencing, was present in human plasma and eluted with plasma miRNAs ..
  21. Aporntewan C, Phokaew C, Piriyapongsa J, Ngamphiw C, Ittiwut C, Tongsima S, et al. Hypomethylation of intragenic LINE-1 represses transcription in cancer cells through AGO2. PLoS ONE. 2011;6:e17934 pubmed publisher
    ..In contrast, many mRNAs derived from genes containing L1s are elevated in Argonaute 2 (AGO2 or EIF2C2)-depleted cells. Hypomethylated L1s increase L1 mRNA levels...
  22. Weinmann L, Höck J, Ivacevic T, Ohrt T, Mütze J, Schwille P, et al. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell. 2009;136:496-507 pubmed publisher
    ..Furthermore, we detect Ago2 in the nucleus of HeLa cells, and knockdown of Imp8 reduces the nuclear Ago2 pool...
  23. Wilson J, Zhang C, Huys A, Richardson C. Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 2011;85:2342-50 pubmed publisher
    ..Argonaute 2 (Ago2) is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed ..
  24. Asai T, Suzuki Y, Matsushita S, Yonezawa S, Yokota J, Katanasaka Y, et al. Disappearance of the angiogenic potential of endothelial cells caused by Argonaute2 knockdown. Biochem Biophys Res Commun. 2008;368:243-8 pubmed publisher
    Argonaute2 (Ago2), a component protein of RNA-induced silencing complex, plays a central role in RNA interference. We focused on the involvement of Ago2 in angiogenesis...
  25. Nelson P, Hatzigeorgiou A, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA. 2004;10:387-94 pubmed
    ..Here we describe that in a human neuronal cell line, the miRNP proteins eIF2C2 (a member of the Argonaute family of proteins), Gemin3, and Gemin4 along with miRNAs cosediment with polyribosomes...
  26. Doi N, Zenno S, Ueda R, Ohki Hamazaki H, Ui Tei K, Saigo K. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Curr Biol. 2003;13:41-6 pubmed
    ..Immunoprecipitation experiments suggest that, in human and mouse cells, complex formation occurs between Dicer and eIF2C1 or 2 and that the PIWI domain of eIF2C is essential for the formation of this complex. ..
  27. Wang D, Zhang Z, O Loughlin E, Lee T, Houel S, O Carroll D, et al. Quantitative functions of Argonaute proteins in mammalian development. Genes Dev. 2012;26:693-704 pubmed publisher
    ..Although Ago2 is the only one with the slicer activity, it is not clear whether the slicer activity is a universally critical ..
  28. Qi H, Ongusaha P, Myllyharju J, Cheng D, Pakkanen O, Shi Y, et al. Prolyl 4-hydroxylation regulates Argonaute 2 stability. Nature. 2008;455:421-4 pubmed publisher
    Human Argonaute (Ago) proteins are essential components of the RNA-induced silencing complexes (RISCs). Argonaute 2 (Ago2) has a P-element-induced wimpy testis (PIWI) domain, which folds like RNase H and is responsible for target RNA ..
  29. Zhou Y, Chen L, Barlogie B, Stephens O, Wu X, Williams D, et al. High-risk myeloma is associated with global elevation of miRNAs and overexpression of EIF2C2/AGO2. Proc Natl Acad Sci U S A. 2010;107:7904-9 pubmed publisher
    ..01) and proliferation index (P < 0.05). Increased expression of EIF2C2/AGO2, a master regulator of the maturation and function of miRNAs and a component of the 70-gene mRNA risk model, ..
  30. Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, et al. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nat Struct Mol Biol. 2007;14:897-903 pubmed
    ..Proteins containing the conserved Ago hook may thus be important regulatory components of effector complexes in RNA interference. ..
  31. Lee Y, Hur I, Park S, Kim Y, Suh M, Kim V. The role of PACT in the RNA silencing pathway. EMBO J. 2006;25:522-32 pubmed
    ..Our study indicates that, unlike other RNase III type proteins, human Dicer may employ two different dsRBD-containing proteins that facilitate RISC assembly. ..
  32. Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell. 2004;15:185-97 pubmed
    ..complexes from different human cell lines revealed that endonuclease activity is exclusively associated with Ago2. Exogenously introduced siRNAs also associate with Ago2 for guiding target RNA cleavage...
  33. Chendrimada T, Gregory R, Kumaraswamy E, Norman J, Cooch N, Nishikura K, et al. TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature. 2005;436:740-4 pubmed
    ..Biochemical analysis of TRBP-containing complexes revealed the association of Dicer-TRBP with Argonaute 2 (Ago2), the catalytic engine of RISC...
  34. Wang H, Noland C, Siridechadilok B, Taylor D, Ma E, Felderer K, et al. Structural insights into RNA processing by the human RISC-loading complex. Nat Struct Mol Biol. 2009;16:1148-53 pubmed publisher
    ..In humans, Argonaute2 (AGO2) assembles with the guide RNA-generating enzyme Dicer and the RNA-binding protein TRBP to form a RISC-loading ..
  35. Sasaki T, Shiohama A, Minoshima S, Shimizu N. Identification of eight members of the Argonaute family in the human genome. Genomics. 2003;82:323-30 pubmed
    ..the PIWI subfamily (PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and PIWIL4/HIWI2) and the eIF2C/AGO subfamily (EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and EIF2C4/hAGO4)...
  36. Sen G, Blau H. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Nat Cell Biol. 2005;7:633-6 pubmed
    ..In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function. ..
  37. Leung A, Calabrese J, Sharp P. Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules. Proc Natl Acad Sci U S A. 2006;103:18125-30 pubmed
    ..Further, miRNAs are required for the Argonaute protein localization to SGs but not PBs. These quantitative kinetic data provide insights into miRNA-mediated repression. ..
  38. Martinez J, Patkaniowska A, Urlaub H, Luhrmann R, Tuschl T. Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Cell. 2002;110:563-74 pubmed
    ..that a single-stranded siRNA resides in the RNA-induced silencing complex (RISC) together with eIF2C1 and/or eIF2C2 (human GERp95) Argonaute proteins...
  39. Lazzaretti D, Tournier I, Izaurralde E. The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins. RNA. 2009;15:1059-66 pubmed publisher
  40. Matranga C, Tomari Y, Shin C, Bartel D, Zamore P. Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell. 2005;123:607-20 pubmed
    In the Drosophila and mammalian RNA interference pathways, siRNAs direct the protein Argonaute2 (Ago2) to cleave corresponding mRNA targets, silencing their expression. Ago2 is the catalytic component of the RNAi enzyme complex, RISC...
  41. Lian S, Li S, Abadal G, Pauley B, Fritzler M, Chan E. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. RNA. 2009;15:804-13 pubmed publisher
    ..b>Ago2 binds miRNA directly and is the core component of miRNA-induced silencing complex...
  42. Maniataki E, Mourelatos Z. A human, ATP-independent, RISC assembly machine fueled by pre-miRNA. Genes Dev. 2005;19:2979-90 pubmed
    ..In humans, the catalytic engine of RISC is a ribonucleoprotein formed by the Argonaute2 (Ago2) protein and either miRNA (miRNP) or siRNA (siRNP)...
  43. Gao M, Wei W, Li M, Wu Y, Ba Z, Jin K, et al. Ago2 facilitates Rad51 recruitment and DNA double-strand break repair by homologous recombination. Cell Res. 2014;24:532-41 pubmed publisher restricted to repair by homologous recombination (HR) and that it specifically relies on the effector protein Ago2 in mammalian cells...
  44. Janowski B, Huffman K, Schwartz J, Ram R, Nordsell R, Shames D, et al. Involvement of AGO1 and AGO2 in mammalian transcriptional silencing. Nat Struct Mol Biol. 2006;13:787-92 pubmed
    ..Here we show that the human homologs of Argonaute-1 (AGO1) and Argonaute-2 (AGO2) link the silencing pathways that target mRNA with pathways mediating recognition of DNA...
  45. Wang B, Li S, Qi H, Chowdhury D, Shi Y, Novina C. Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat Struct Mol Biol. 2009;16:1259-66 pubmed publisher
    ..Using a minimal in vitro system, we show that bacterially expressed human AGO1 and AGO2 but not AGO3 and AGO4 possess strand-dissociating activity of microRNA (miRNA) duplexes...
  46. Adams B, Claffey K, White B. Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 2009;150:14-23 pubmed publisher
    ..Ago) 2 is the catalytic engine of mammalian RNA interference, but little is known concerning the regulation of Ago2 by cell-signaling pathways...
  47. Elkayam E, Kuhn C, Tocilj A, Haase A, Greene E, Hannon G, et al. The structure of human argonaute-2 in complex with miR-20a. Cell. 2012;150:100-10 pubmed publisher
    ..Here, we report the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 Å resolution...
  48. Macrae I, Ma E, Zhou M, Robinson C, Doudna J. In vitro reconstitution of the human RISC-loading complex. Proc Natl Acad Sci U S A. 2008;105:512-7 pubmed publisher
    ..In humans, Ago2 is loaded with miRNAs by the action of a specialized assembly called the RISC-loading complex (RLC), comprising the ..
  49. Pfaff J, Hennig J, Herzog F, Aebersold R, Sattler M, Niessing D, et al. Structural features of Argonaute-GW182 protein interactions. Proc Natl Acad Sci U S A. 2013;110:E3770-9 pubmed publisher
    ..Using cross-linking followed by mass spectrometry, we map the GW protein interactions with Ago2, which allows for structural modeling of Ago-GW182 interaction...
  50. Hatanaka K, Shimizu K, Asai T, Oku N. [Antineovascular gene therapy by Ago2 knockdown]. Yakugaku Zasshi. 2008;128:1567-75 pubmed
    ..In the present study, we optimized PCL for siRNA transfection and used it to determine the role of Argonaute2 (Ago2), a main constitution protein of RNA-induced silencing complex (RISC), on angiogenesis...
  51. Yoda M, Kawamata T, Paroo Z, Ye X, Iwasaki S, Liu Q, et al. ATP-dependent human RISC assembly pathways. Nat Struct Mol Biol. 2010;17:17-23 pubmed publisher
    ..All these features of human AGO proteins are highly reminiscent of fly Ago1 but not fly Ago2.
  52. Liu J, Valencia Sanchez M, Hannon G, Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat Cell Biol. 2005;7:719-23 pubmed
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  53. Huang V, Zheng J, Qi Z, Wang J, Place R, Yu J, et al. Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells. PLoS Genet. 2013;9:e1003821 pubmed publisher
    ..Our findings reveal the first landscape of human Ago1-chromosomal interactions, which may play a role in the oncogenic transcriptional program of cancer cells. ..
  54. Wakiyama M, Takimoto K, Ohara O, Yokoyama S. Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system. Genes Dev. 2007;21:1857-62 pubmed
    ..Our work suggests that let-7 miRNPs containing Argonaute and GW182 impair the synergistic enhancement of translation by the 5'-cap and 3'-poly(A) tail, resulting in translational repression. ..
  55. Nathans R, Chu C, Serquina A, Lu C, Cao H, Rana T. Cellular microRNA and P bodies modulate host-HIV-1 interactions. Mol Cell. 2009;34:696-709 pubmed publisher
    ..Thus we provide an example of a single host miRNA regulating HIV-1 production and infectivity. These studies highlight the significance of miRNAs and P bodies in modulating host cell interactions with HIV-1 and possibly other viruses. ..
  56. Gu S, Jin L, Huang Y, Zhang F, Kay M. Slicing-independent RISC activation requires the argonaute PAZ domain. Curr Biol. 2012;22:1536-42 pubmed publisher
  57. James V, Zhang Y, Foxler D, de Moor C, Kong Y, Webb T, et al. LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proc Natl Acad Sci U S A. 2010;107:12499-504 pubmed publisher
  58. Jing Q, Huang S, Guth S, Zarubin T, Motoyama A, Chen J, et al. Involvement of microRNA in AU-rich element-mediated mRNA instability. Cell. 2005;120:623-34 pubmed
    ..An RNAi-based screen performed in Drosophila S2 cells has revealed that Dicer1, Argonaute1 (Ago1) and Ago2, components involved in microRNA (miRNA) processing and function, are required for the rapid decay of mRNA ..
  59. Kraynack B, Baker B. Small interfering RNAs containing full 2'-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity. RNA. 2006;12:163-76 pubmed
    ..We further show, by using biotin-tagged siRNAs and affinity-tagged hAgo2/eIF2C2, that activity of siRNA duplexes containing full 2'-OMe substitutions in the sense strand is mediated by the RNA-..
  60. Choe J, Cho H, Chi S, Kim Y. Ago2/miRISC-mediated inhibition of CBP80/20-dependent translation and thereby abrogation of nonsense-mediated mRNA decay require the cap-associating activity of Ago2. FEBS Lett. 2011;585:2682-7 pubmed publisher
    ..Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is ..
  61. Benhamed M, Herbig U, Ye T, Dejean A, Bischof O. Senescence is an endogenous trigger for microRNA-directed transcriptional gene silencing in human cells. Nat Cell Biol. 2012;14:266-75 pubmed publisher
    ..Here, we demonstrate that AGO2, RB1 and microRNAs (miRNAs), as exemplified here by let-7, physically and functionally interact to repress RB1/E2F-..
  62. Ameyar Zazoua M, Rachez C, Souidi M, Robin P, Fritsch L, Young R, et al. Argonaute proteins couple chromatin silencing to alternative splicing. Nat Struct Mol Biol. 2012;19:998-1004 pubmed publisher
    ..Here, we have immunopurified human Argonaute-1 and Argonaute-2 (AGO1 and AGO2) chromatin-embedded proteins and found them associated with chromatin modifiers and, notably, with splicing factors...
  63. Chendrimada T, Finn K, Ji X, Baillat D, Gregory R, Liebhaber S, et al. MicroRNA silencing through RISC recruitment of eIF6. Nature. 2007;447:823-8 pubmed
    ..These results uncover an evolutionarily conserved function of the ribosome anti-association factor eIF6 in miRNA-mediated post-transcriptional silencing. ..
  64. Koesters R, Adams V, Betts D, Moos R, Schmid M, Siermann A, et al. Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. Genomics. 1999;61:210-8 pubmed
    ..It is highly conserved during evolution, sharing about 90% identity with rabbit eIF2C and 70% identity with plant AGO1 at the amino acid level. These facts suggest that human EIF2C1 might play an important physiological role. ..
  65. De N, Young L, Lau P, Meisner N, Morrissey D, Macrae I. Highly complementary target RNAs promote release of guide RNAs from human Argonaute2. Mol Cell. 2013;50:344-55 pubmed publisher
    ..Here we show that the Argonaute2 (Ago2) guide RNA complex is extremely stable, with a half-life on the order of days...
  66. Carmell M, Xuan Z, Zhang M, Hannon G. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. Genes Dev. 2002;16:2733-42 pubmed
  67. Rüdel S, Wang Y, Lenobel R, Körner R, Hsiao H, Urlaub H, et al. Phosphorylation of human Argonaute proteins affects small RNA binding. Nucleic Acids Res. 2011;39:2330-43 pubmed publisher
    ..Here we report a comprehensive analysis of Ago2 phosphorylation in human cells...
  68. Wichroski M, Robb G, Rana T. Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. PLoS Pathog. 2006;2:e41 pubmed
    ..and eIF4E-T), translation suppression (RCK/p54), RNA interference-mediated post-transcriptional gene silencing (AGO2), and decapping of mRNA (DCP2)...
  69. Shimakami T, Yamane D, Jangra R, Kempf B, Spaniel C, Barton D, et al. Stabilization of hepatitis C virus RNA by an Ago2-miR-122 complex. Proc Natl Acad Sci U S A. 2012;109:941-6 pubmed publisher
    ..Here we show that miR-122 binds HCV RNA in association with Ago2 and that this slows decay of the viral genome in infected cells...
  70. Horikawa Y, Wood C, Yang H, Zhao H, Ye Y, Gu J, et al. Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma. Clin Cancer Res. 2008;14:7956-62 pubmed publisher
    ..nucleotide polymorphisms (SNP) from 11 miRNA processing genes (DROSHA, DGCR8, XPO5, RAN, DICER1, TARBP2, AGO1, AGO2, GEMIN3, GEMIN4, HIWI) and 15 miRNA genes in 279 Caucasian patients with renal cell carcinoma and 278 matched ..
  71. Horman S, Janas M, Litterst C, Wang B, Macrae I, Sever M, et al. Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013;50:356-67 pubmed publisher
    ..Here we report that the proto-oncogene Akt-3/PKB? (Akt3) phosphorylates Argonaute 2 (Ago2) at S387, which downregulates cleavage and upregulates translational repression of endogenous microRNA (miRNA)-..