AGO1

Summary

Gene Symbol: AGO1
Description: argonaute 1, RISC catalytic component
Alias: EIF2C, EIF2C1, GERP95, Q99, hAgo1, protein argonaute-1, Golgi Endoplasmic Reticulum protein 95 kDa, argonaute RISC catalytic component 1, argonaute1, eIF-2C 1, eIF2C 1, eukaryotic translation initiation factor 2C, 1
Species: human
Products:     AGO1

Top Publications

  1. Sasaki T, Shiohama A, Minoshima S, Shimizu N. Identification of eight members of the Argonaute family in the human genome. Genomics. 2003;82:323-30 pubmed
    ..subfamilies: the PIWI subfamily (PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and PIWIL4/HIWI2) and the eIF2C/AGO subfamily (EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and EIF2C4/hAGO4)...
  2. Höck J, Weinmann L, Ender C, Rüdel S, Kremmer E, Raabe M, et al. Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. EMBO Rep. 2007;8:1052-60 pubmed
    ..Here, we analysed Ago1- and Ago2-containing protein complexes in human cells...
  3. Horikawa Y, Wood C, Yang H, Zhao H, Ye Y, Gu J, et al. Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma. Clin Cancer Res. 2008;14:7956-62 pubmed publisher
    ..40 single nucleotide polymorphisms (SNP) from 11 miRNA processing genes (DROSHA, DGCR8, XPO5, RAN, DICER1, TARBP2, AGO1, AGO2, GEMIN3, GEMIN4, HIWI) and 15 miRNA genes in 279 Caucasian patients with renal cell carcinoma and 278 matched ..
  4. Sen G, Blau H. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Nat Cell Biol. 2005;7:633-6 pubmed
    ..In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function. ..
  5. Kim D, Villeneuve L, Morris K, Rossi J. Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells. Nat Struct Mol Biol. 2006;13:793-7 pubmed
    ..Here we describe the role of the human Argonaute-1 homolog (AGO1) in directing TGS at the promoters for human immunodeficiency virus-1 coreceptor CCR5 and tumor suppressor RASSF1A...
  6. Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell. 2004;15:185-97 pubmed
    ..The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types...
  7. Liu J, Valencia Sanchez M, Hannon G, Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat Cell Biol. 2005;7:719-23 pubmed
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  8. Wang B, Li S, Qi H, Chowdhury D, Shi Y, Novina C. Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat Struct Mol Biol. 2009;16:1259-66 pubmed publisher
    ..Using a minimal in vitro system, we show that bacterially expressed human AGO1 and AGO2 but not AGO3 and AGO4 possess strand-dissociating activity of microRNA (miRNA) duplexes...
  9. Ameyar Zazoua M, Rachez C, Souidi M, Robin P, Fritsch L, Young R, et al. Argonaute proteins couple chromatin silencing to alternative splicing. Nat Struct Mol Biol. 2012;19:998-1004 pubmed publisher
    ..Here, we have immunopurified human Argonaute-1 and Argonaute-2 (AGO1 and AGO2) chromatin-embedded proteins and found them associated with chromatin modifiers and, notably, with ..

More Information

Publications119 found, 100 shown here

  1. Meister G, Landthaler M, Peters L, Chen P, Urlaub H, Luhrmann R, et al. Identification of novel argonaute-associated proteins. Curr Biol. 2005;15:2149-55 pubmed
    ..To gain insight into the function and composition of RNA silencing complexes, we purified Ago1- and Ago2-containing complexes from human cells...
  2. Wang D, Zhang Z, O Loughlin E, Lee T, Houel S, O Carroll D, et al. Quantitative functions of Argonaute proteins in mammalian development. Genes Dev. 2012;26:693-704 pubmed publisher
    Argonaute proteins (Ago1-4) are essential components of the microRNA-induced silencing complex and play important roles in both microRNA biogenesis and function...
  3. Gu S, Jin L, Huang Y, Zhang F, Kay M. Slicing-independent RISC activation requires the argonaute PAZ domain. Curr Biol. 2012;22:1536-42 pubmed publisher
    ..the RNA-induced silencing complex (RISC), which, in mammals, contains at its center one of four Argonaute proteins (Ago1-Ago4)...
  4. Müller C, Schäfer I, Luhmann H, White R. Oligodendroglial Argonaute protein Ago2 associates with molecules of the Mbp mRNA localization machinery and is a downstream target of Fyn kinase. Front Cell Neurosci. 2015;9:328 pubmed publisher
    ..We show that all Ago proteins (Ago1-4) are expressed by oligodendrocytes and that Ago2 colocalizes with hnRNP A2 in granular cytoplasmic structures...
  5. Tao W, Sun L, Chen J, Shi H, Wang D. Genomic identification, rapid evolution, and expression of Argonaute genes in the tilapia, Oreochromis niloticus. Dev Genes Evol. 2016;226:339-48 pubmed publisher
    ..b>Ago1 and Ago4 were detected at higher levels at 5 days after hatching (dah) in both ovaries and testes compared with ..
  6. Yoshikawa M, Iki T, Numa H, Miyashita K, Meshi T, Ishikawa M. A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis. Plant Physiol. 2016;171:359-68 pubmed publisher
    ..the 3' fragment of pri-TAS2, which is a source of tasiRNAs, forms a huge complex containing SGS3, miR173-programmed AGO1 RNA-induced silencing complex, the 5' fragment, and ribosomes...
  7. Wang T, Zhang S, Cao S, Guo W, Yan B, Fang H. Protective effects of salubrinal on liver injury in rat models of brain death. Chin Med J (Engl). 2015;128:1523-8 pubmed publisher
    ..Compared with BD group, Sal group had a significantly higher P-eIF2C level and a lower P-PERK level 2 h and 6 h after BD (P < 0.05)...
  8. Eckenfelder A, Segeral E, Pinzón N, Ulveling D, Amadori C, Charpentier M, et al. Argonaute proteins regulate HIV-1 multiply spliced RNA and viral production in a Dicer independent manner. Nucleic Acids Res. 2017;45:4158-4173 pubmed publisher
    ..Furthermore, inhibition of Ago1 and Ago2 levels in cells expressing HIV-1 led to an increase of viral multiply spliced transcripts and to a strong ..
  9. Ghosh S, Kakumani P, Kumar A, Malhotra P, Mukherjee S, Bhatnagar R. Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins. BMC Genomics. 2014;15:775 pubmed publisher
    ..Understanding the interior involved in different phases of gene silencing also offers impending tool for RNAi-based applications. ..
  10. Zheng Y, Guo X, He W, Shao Z, Zhang X, Yang J, et al. Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells. Int Immunopharmacol. 2016;34:259-262 pubmed publisher
    ..However, the role of miR-71 during infection remains poorly characterized. Herein, we show that Ago1 and Ago4, which encode key components of the small RNA-induced silencing complex (RISC), were up-regulated in ..
  11. Ho T, Panyim S, Udomkit A. Suppression of argonautes compromises viral infection in Penaeus monodon. Dev Comp Immunol. 2019;90:130-137 pubmed publisher
    ..Two PmAgo proteins, PmAgo1 and PmAgo3 shared high percentage of amino acid identity to Ago1 and Ago2, respectively in other Penaeid shrimps...
  12. Bejerman N, Mann K, Dietzgen R. Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing. Virus Res. 2016;224:19-28 pubmed publisher
    ..The mechanism of suppression appears to involve ADV P binding to RNA-induced silencing complex proteins AGO1 and AGO4 as shown in protein-protein interaction assays when ectopically expressed...
  13. Xiong X, Vogler G, Kurthkoti K, Samsonova A, Zhou R. SmD1 Modulates the miRNA Pathway Independently of Its Pre-mRNA Splicing Function. PLoS Genet. 2015;11:e1005475 pubmed publisher
    ..Moreover, SmD1 physically and functionally associates with components of the miRISC, including AGO1 and GW182...
  14. Mugat B, Akkouche A, Serrano V, Armenise C, Li B, Brun C, et al. MicroRNA-Dependent Transcriptional Silencing of Transposable Elements in Drosophila Follicle Cells. PLoS Genet. 2015;11:e1005194 pubmed publisher
    ..Global miRNA depletion, caused by tissue- and stage-specific knock down of drosha (involved in miRNA biogenesis), AGO1 or gawky (both responsible for miRNA activity), resulted in loss of TE-derived piRNAs and chromatin-mediated ..
  15. Wang S, Zheng Y, Gu C, He C, Yang M, Zhang X, et al. Bacillus cereus AR156 activates defense responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana similarly to flg22. Mol Plant Microbe Interact. 2017;: pubmed publisher
    ..On the other hand, AR156 and flg22 acted in concert to differentially regulate a number of AGO1-bound miRNAs that function to mediate PTI...
  16. Wu H, Li S, Hu J, Yu X, Xu H, Chen Z, et al. Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells. J Exp Clin Cancer Res. 2016;35:145 pubmed publisher
    ..Additionally, ChIP assays indicated that p21 activation was accompanied by an increased enrichment of AGO1 and the trimethylation of histone H3K4 at dsRNA-targeted genomic sites...
  17. Pumplin N, Sarazin A, Jullien P, Bologna N, Oberlin S, Voinnet O. DNA Methylation Influences the Expression of DICER-LIKE4 Isoforms, Which Encode Proteins of Alternative Localization and Function. Plant Cell. 2016;28:2786-2804 pubmed
    ..originate mostly from transposable elements (TEs) and TE-overlapping/proximal genes, load into the PTGS effector ARGONAUTE1 (AGO1), and display a subtle effect on transcript accumulation together with overlapping 24-nucleotide siRNAs...
  18. Haac M, Anderson M, Eggleston H, Myles K, Adelman Z. The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes. Nucleic Acids Res. 2015;43:3688-700 pubmed publisher
  19. Brosseau C, El Oirdi M, Adurogbangba A, Ma X, Moffett P. Antiviral Defense Involves AGO4 in an Arabidopsis-Potexvirus Interaction. Mol Plant Microbe Interact. 2016;29:878-888 pubmed
    ..multiple AGO proteins with specialized functions, including AGO4-like proteins that affect RdDM and AGO2, AGO5, and AGO1, which have antiviral activities...
  20. Wang X, Wang Y, Dou Y, Chen L, Wang J, Jiang N, et al. Degradation of unmethylated miRNA/miRNA*s by a DEDDy-type 3' to 5' exoribonuclease Atrimmer 2 in Arabidopsis. Proc Natl Acad Sci U S A. 2018;115:E6659-E6667 pubmed publisher
    ..Finally, we show that ATRM2 colocalizes and physically interacts with Argonaute 1 (AGO1)...
  21. Okazaki K, Kato H, Iida T, Shinmyozu K, Nakayama J, Murakami Y, et al. RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5. Epigenetics Chromatin. 2018;11:26 pubmed publisher
    ..the level of dimethylation of histone H3 at Lys9 at the pericentromere, where association of the Argonaute protein Ago1 was also abrogated...
  22. Navas P, Paffoni A, Intra G, González Utor A, Clavero A, Gonzalvo M, et al. Obstetric and neo-natal outcomes of ICSI cycles using pentoxifylline to identify viable spermatozoa in patients with immotile spermatozoa. Reprod Biomed Online. 2017;34:414-421 pubmed publisher
    ..classified according to the World Health Organization International Classification of Diseases (ICD-10, range Q00-Q99)...
  23. Yoshikawa M, Iki T, Tsutsui Y, Miyashita K, Poethig R, Habu Y, et al. 3' fragment of miR173-programmed RISC-cleaved RNA is protected from degradation in a complex with RISC and SGS3. Proc Natl Acad Sci U S A. 2013;110:4117-22 pubmed publisher
    ..These results suggest that (i) SGS3 associates with AGO1-RISC via the double-stranded RNA formed by the 3'-terminal nucleotides of 22-nt miR173 and corresponding target RNA,..
  24. Jin H, Suh M, Han J, Yeom K, Lee Y, Heo I, et al. Human UPF1 participates in small RNA-induced mRNA downregulation. Mol Cell Biol. 2009;29:5789-99 pubmed publisher
    ..The helicase domain mutant of hUPF1 fails to suppress miRNA targets. hUPF1 interacts with human Argonaute 1 (hAGO1) and hAGO2 and colocalizes with hAGO1 and hAGO2 in processing bodies, which are known to be the sites for ..
  25. Besnard Guérin C, Jacquier C, Pidoux J, Deddouche S, Antoniewski C, Antoniewsk C. The cricket paralysis virus suppressor inhibits microRNA silencing mediated by the Drosophila Argonaute-2 protein. PLoS ONE. 2015;10:e0120205 pubmed publisher
    ..Thus, our results provide the first evidence that insect VSR have the potential to directly interfere with the miRNA silencing pathway. ..
  26. Ellahi A, Rine J. Evolution and Functional Trajectory of Sir1 in Gene Silencing. Mol Cell Biol. 2016;36:1164-79 pubmed publisher
    ..In contrast to the prominent role of Sir proteins in silencing, T. delbrueckii RNAi genes AGO1 and DCR1 did not function in heterochromatin formation...
  27. Peredo J, Villacé P, Ortín J, de Lucas S. Human Staufen1 associates to miRNAs involved in neuronal cell differentiation and is required for correct dendritic formation. PLoS ONE. 2014;9:e113704 pubmed publisher
    ..we show that human Staufen1-containing complexes contain essential elements of the gene silencing apparatus, like Ago1-3 proteins, and we describe a set of miRNAs specifically associated to complexes containing human Staufen1...
  28. Sellars M, Trewin C, McWilliam S, Glaves R, Hertzler P. Transcriptome profiles of Penaeus (Marsupenaeus) japonicus animal and vegetal half-embryos: identification of sex determination, germ line, mesoderm, and other developmental genes. Mar Biotechnol (NY). 2015;17:252-65 pubmed publisher
    ..Genes expressed only in the animal half included bmp1, kruppel, maelstrom, and orthodenticle. Genes expressed only in the vegetal half included boule, brachyury, deformed, dorsal, engrailed, hunchback, spalt, twist, and wingless/wnt-8c. ..
  29. Mallamaci A. Enhancing Neuronogenesis and Counteracting Neuropathogenic Gene Haploinsufficiencies by RNA Gene Activation. Adv Exp Med Biol. 2017;983:23-39 pubmed publisher
    ..Foxg1-activating miRNAs stimulated RNApolII recruitment, possibly via Ago1. One of them worked promisingly in vivo...
  30. Ma X, Nicole M, Meteignier L, Hong N, Wang G, Moffett P. Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants. J Exp Bot. 2015;66:919-32 pubmed publisher
    ..for AGO2 and AGO4 in susceptibility to TRV, whereas VIGS of endogenous genes appeared to be largely mediated by AGO1. However, recovery appeared to be mediated by different components, as all the aforementioned mutants were able to ..
  31. Vinutha T, Kumar G, Garg V, Canto T, Palukaitis P, Ramesh S, et al. Tomato geminivirus encoded RNAi suppressor protein, AC4 interacts with host AGO4 and precludes viral DNA methylation. Gene. 2018;678:184-195 pubmed publisher
    ..AC4 showed interaction with host AGO4 but not with AGO1, aggregated around the nucleus, and influenced cytosine methylation of the viral genome...
  32. Ma J, Ye K, Patel D. Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain. Nature. 2004;429:318-322 pubmed publisher
    ..Here, we report the 2.6 A crystal structure of the PAZ domain from human Argonaute eIF2c1 bound to both ends of a 9-mer siRNA-like duplex...
  33. Fabian M, Mathonnet G, Sundermeier T, Mathys H, Zipprich J, Svitkin Y, et al. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009;35:868-80 pubmed publisher
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation...
  34. Asman A, Fogelqvist J, Vetukuri R, Dixelius C. Phytophthora infestans Argonaute 1 binds microRNA and small RNAs from effector genes and transposable elements. New Phytol. 2016;211:993-1007 pubmed publisher
    ..The data suggest that PiAgo1 plays an essential role in gene regulation and that at least two RNA silencing pathways regulate TEs in the plant-pathogenic oomycete P. infestans. ..
  35. Anaparti V, Smolik I, Meng X, Spicer V, Mookherjee N, El Gabalawy H. Whole blood microRNA expression pattern differentiates patients with rheumatoid arthritis, their seropositive first-degree relatives, and healthy unrelated control subjects. Arthritis Res Ther. 2017;19:249 pubmed publisher
    ..Consistent with these curated networks, mRNA expression of DICER1, AGO1, CREB1, DAPK1, and TP53 was downregulated significantly with miR-103a-3p expression in FDRs...
  36. Zhang H, Xia R, Meyers B, Walbot V. Evolution, functions, and mysteries of plant ARGONAUTE proteins. Curr Opin Plant Biol. 2015;27:84-90 pubmed publisher
    ..The Arabidopsis thaliana genome encodes ten AGO proteins, designated AGO1 to AGO10. Most early studies focused on these ten proteins and their interacting sRNAs...
  37. Ghosh U, Adhya S. Non-equivalent Roles of AGO1 and AGO2 in mRNA Turnover and Translation of Cyclin D1 mRNA. J Biol Chem. 2016;291:7119-27 pubmed publisher
    Mammalian Argonaute proteins (AGO1-4), in combination with microRNAs (miRs), bind to target mRNAs to initiate degradation and/or translation repression, but the relationships between these two effects is unclear...
  38. Luo H, Li X, Claycomb J, Lipshitz H. The Smaug RNA-Binding Protein Is Essential for microRNA Synthesis During the Drosophila Maternal-to-Zygotic Transition. G3 (Bethesda). 2016;6:3541-3551 pubmed publisher
    ..SMG is also required for the synthesis and stability of a key miRISC component, Argonaute 1 (AGO1), but plays no role in accumulation of the Argonaute family proteins associated with piRNAs or siRNAs...
  39. Eini O. [A Betasatellite-Encoded Protein Regulates Key Components of Gene Silencing System in Plants]. Mol Biol (Mosk). 2017;51:656-663 pubmed publisher
    ..of transgenic plants expressing CLCuMuB βC1 using real-time PCR showed that the expression levels of both AGO1 and DCL1 genes were significantly increased...
  40. Salvi S, Conteduca V, Martignano F, Gurioli G, Calistri D, Casadio V. Serum and Plasma Copy Number Detection Using Real-time PCR. J Vis Exp. 2017;: pubmed publisher
    ..We evaluated AR CN in cfDNA using 2 different real-time PCR assays and 2 reference genes (RNaseP and AGO1). DNA amount of 60 ng was used for each assay combination...
  41. Phalora P, Sherer N, Wolinsky S, Swanson C, Malim M. HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies. J Virol. 2012;86:11712-24 pubmed publisher
    ..Localization to P bodies may therefore provide a means of sequestering APOBEC3 enzymatic activity away from cellular DNA or may be linked to as yet unidentified cellular functions. ..
  42. Chattopadhyay M, Stupina V, Gao F, Szarko C, Kuhlmann M, Yuan X, et al. Requirement for Host RNA-Silencing Components and the Virus-Silencing Suppressor when Second-Site Mutations Compensate for Structural Defects in the 3' Untranslated Region. J Virol. 2015;89:11603-18 pubmed publisher
    ..were not compensatory in the absence of p38, which is also the TCV silencing suppressor, or in dcl-2/dcl4 or ago1/ago2 backgrounds...
  43. Park M, Phan H, Busch F, Hinckley S, Brackbill J, Wysocki V, et al. Human Argonaute3 has slicer activity. Nucleic Acids Res. 2017;45:11867-11877 pubmed publisher
    Of the four human Argonaute (AGO) paralogs, only AGO2 has been shown to have slicer activity. The others (AGO1, AGO3 and AGO4) have been thought to assemble with microRNAs to form slicer-independent effector complexes that bind target ..
  44. Weinmann L, Höck J, Ivacevic T, Ohrt T, Mütze J, Schwille P, et al. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell. 2009;136:496-507 pubmed publisher
    ..Therefore, we provide evidence that Imp8 is required for cytoplasmic miRNA-guided gene silencing and affects nuclear localization of Ago proteins. ..
  45. Chen Z, Lai T, Jan Y, Lin F, Wang W, Xiao H, et al. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. J Clin Invest. 2013;123:1057-67 pubmed publisher
    ..silico bioinformatics and in vitro validation showed that these HRMs are induced by HIF1? and target argonaute 1 (AGO1), which anchors the microRNA-induced silencing complex (miRISC)...
  46. Qin J, Ma X, Yi Z, Tang Z, Meng Y. A transcriptome-wide study on the microRNA- and the Argonaute 1-enriched small RNA-mediated regulatory networks involved in plant leaf senescence. Plant Biol (Stuttg). 2016;18:197-205 pubmed publisher
    ..Besides, whether other Argonaute 1 (AGO1)-enriched small RNAs (sRNAs) play regulatory roles in leaf senescence remains unclear...
  47. Wang T, Li R, Wen L, Fu D, Zhu B, Luo Y, et al. Functional Analysis and RNA Sequencing Indicate the Regulatory Role of Argonaute1 in Tomato Compound Leaf Development. PLoS ONE. 2015;10:e0140756 pubmed publisher
    ..The role of Argonaute1 (AGO1) in the development of simple leaves has been established, but its role in the development of compound ..
  48. Sun S, Ma J, Zhang Q, Wang Q, Zhou L, Bai F, et al. Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis. Cardiovasc Pathol. 2016;25:120-6 pubmed publisher
    ..In this study, we detected the expression of four AGOs in the CVB3-infected mouse heart tissues and found that AGO1 and AGO3 up-regulated significantly at 4 and 8h after CVB3 infection...
  49. Arribas Hernández L, Kielpinski L, Brodersen P. mRNA Decay of Most Arabidopsis miRNA Targets Requires Slicer Activity of AGO1. Plant Physiol. 2016;171:2620-32 pubmed publisher
    ..Here, we compare phenotypes and transcript profiles of ago1 null and slicer-deficient mutants in Arabidopsis (Arabidopsis thaliana)...
  50. Park D, Kim H, Kim Y, Jeoung D. miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs. Mol Cells. 2016;39:299-309 pubmed publisher
    ..miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217...
  51. Dolata J, Bajczyk M, Bielewicz D, Niedojadlo K, Niedojadlo J, Pietrykowska H, et al. Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels. Plant Physiol. 2016;172:297-312 pubmed publisher
    ..b>ARGONAUTE1 (AGO1) participated in both processes...
  52. Fimiani C, Goina E, Su Q, Gao G, Mallamaci A. RNA activation of haploinsufficient Foxg1 gene in murine neocortex. Sci Rep. 2016;6:39311 pubmed publisher
    ..saRNAs recognized target chromatin through a ncRNA stemming from it. Gene upregulation required Ago1 and was associated to RNApolII enrichment throughout the Foxg1 locus...
  53. Qin J, Tang Z, Ma X, Meng Y. Investigating the regulatory roles of the microRNAs and the Argonaute 1-enriched small RNAs in plant metabolism. Gene. 2017;628:180-189 pubmed publisher
    ..sequencing data, large-scale target identification was performed for microRNAs (miRNAs) and Argonaute 1 (AGO1)-enriched sRNAs in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa)...
  54. Ngondo R, Cirera Salinas D, Yu J, Wischnewski H, Bodak M, Vandormael Pournin S, et al. Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018;10:461-476 pubmed publisher
    ..autonomous and can be rescued by both a catalytically active and an inactive Ago2, but not by Ago2 deprived of its RNA binding capacity or by Ago1 overexpression. Overall, our results suggest a role for AGO2 in stem cell differentiation.
  55. Lazzaretti D, Tournier I, Izaurralde E. The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins. RNA. 2009;15:1059-66 pubmed publisher
    ..the N-terminal GW-repeat-containing regions of all three TNRC6s interact with the four human Argonaute proteins (AGO1-AGO4). We also show that TNRC6A, TNRC6B, and TNRC6C silence the expression of bound mRNAs...
  56. Yu Y, Ji L, Le B, Zhai J, Chen J, Luscher E, et al. ARGONAUTE10 promotes the degradation of miR165/6 through the SDN1 and SDN2 exonucleases in Arabidopsis. PLoS Biol. 2017;15:e2001272 pubmed publisher
    ..b>AGO1 is the microRNA (miRNA) effector in Arabidopsis, and its closest homolog, AGO10, maintains stem cell homeostasis in ..
  57. de Felippes F, Marchais A, Sarazin A, Oberlin S, Voinnet O. A single miR390 targeting event is sufficient for triggering TAS3-tasiRNA biogenesis in Arabidopsis. Nucleic Acids Res. 2017;45:5539-5554 pubmed publisher
    ..production proceeds via a single cleavage event mediated by 22nt-long or/and asymmetric miRNAs in an ARGONAUTE-1 (AGO1)-dependent manner...
  58. Hessam S, Sand M, Skrygan M, Bechara F. The microRNA effector RNA-induced silencing complex in hidradenitis suppurativa: a significant dysregulation within active inflammatory lesions. Arch Dermatol Res. 2017;309:557-565 pubmed publisher
    ..TRBP2, protein activator (PACT) of the interferon-induced protein kinase R, Argonaute RISC Catalytic Component-1 (AGO1) and Component-2 (AGO2), metadherin, and staphylococcal nuclease and Tudor domain-containing-1 (SND1) in inflamed ..
  59. Freimer J, Krishnakumar R, Cook M, Blelloch R. Expression of Alternative Ago2 Isoform Associated with Loss of microRNA-Driven Translational Repression in Mouse Oocytes. Curr Biol. 2018;28:296-302.e3 pubmed publisher
    ..Full-length Ago2 as well as the related Argonautes (Ago1, Ago3, and Ago4) were lowly expressed in maturing mouse oocytes...
  60. Jønson L, Christiansen J, Hansen T, Vikeså J, Yamamoto Y, Nielsen F. IMP3 RNP safe houses prevent miRNA-directed HMGA2 mRNA decay in cancer and development. Cell Rep. 2014;7:539-551 pubmed publisher
    ..Removal of let-7 target sites eliminates IMP3-dependent stabilization, and IMP3-containing bodies are depleted of Ago1-4 and miRNAs...
  61. Goldie B, Fitzsimmons C, Weidenhofer J, Atkins J, Wang D, Cairns M. miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif. Front Mol Neurosci. 2017;10:259 pubmed publisher
    ..Precursor miRNA containing this motif were shown to have affinity for MAZ protein in nuclear extract. We then used Ago1/2 RIP-Seq to explore nuclear miRNA-associated mRNA targets...
  62. Lian S, Li S, Abadal G, Pauley B, Fritzler M, Chan E. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. RNA. 2009;15:804-13 pubmed publisher
  63. Hausser J, Landthaler M, Jaskiewicz L, Gaidatzis D, Zavolan M. Relative contribution of sequence and structure features to the mRNA binding of Argonaute/EIF2C-miRNA complexes and the degradation of miRNA targets. Genome Res. 2009;19:2009-20 pubmed publisher
    ..features play a role, we transfected human HEK293 cells with miRNAs and analyzed the association of Argonaute/EIF2C-miRNA complexes with target mRNAs and the degradation of these messages...
  64. Faehnle C, Elkayam E, Haase A, Hannon G, Joshua Tor L. The making of a slicer: activation of human Argonaute-1. Cell Rep. 2013;3:1901-9 pubmed publisher
    Argonautes are the central protein component in small RNA silencing pathways. Of the four human Argonautes (hAgo1-hAgo4) only hAgo2 is an active slicer. We determined the structure of hAgo1 bound to endogenous copurified RNAs to 1...
  65. Nakanishi K, Ascano M, Gogakos T, Ishibe Murakami S, Serganov A, Briskin D, et al. Eukaryote-specific insertion elements control human ARGONAUTE slicer activity. Cell Rep. 2013;3:1893-900 pubmed publisher
    We have solved the crystal structure of human ARGONAUTE1 (hAGO1) bound to endogenous 5'-phosphorylated guide RNAs...
  66. Tang S, Chen Y, Feng S, Yi T, Liu X, Li Q, et al. MiR-483-5p promotes IGF-II transcription and is associated with poor prognosis of hepatocellular carcinoma. Oncotarget. 2017;8:99871-99888 pubmed publisher
    ..The mechanism was involved in recruiting of an argonaute 1(Ago1)-argonaute 2 (Ago2) complex to the P3 mRNA 5'UTR and the P3 promoter of IGF-II gene by miR-483-5p, accompanied by ..
  67. Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, et al. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nat Struct Mol Biol. 2007;14:897-903 pubmed
    Argonaute (Ago) proteins mediate silencing of nucleic acid targets by small RNAs. In fission yeast, Ago1, Tas3 and Chp1 assemble into a RITS complex, which silences transcription near centromeres...
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    ..Future investigation in larger populations and functional characterizations are necessary to validate these results. ..
  69. Zhang L, Ding Y, Yuan Z, Liu J, Sun J, Lei F, et al. MicroRNA-500 sustains nuclear factor-κB activation and induces gastric cancer cell proliferation and resistance to apoptosis. Oncotarget. 2015;6:2483-95 pubmed
    ..Hence, we report the uncovering of a novel mechanism for constitutive NF-κB activation, indicating the potentially pivotal role of miR-500 in the progression of gastric cancer. ..
  70. Jin X, Guo X, Zhu D, Ayaz M, Zheng Y. miRNA profiling in the mice in response to Echinococcus multilocularis infection. Acta Trop. 2017;166:39-44 pubmed publisher
    ..infection disturbed the expression of 4 of 10 genes essential to miRNA biogenesis in the mouse liver, including ago1, ago4, tarbp2 and xrn2...
  71. Song X, Zhong H, Wu Q, Wang M, Zhou J, Zhou Y, et al. Association between SNPs in microRNA machinery genes and gastric cancer susceptibility, invasion, and metastasis in Chinese Han population. Oncotarget. 2017;8:86435-86446 pubmed publisher
    The present study investigates the influence of genetic variants in miRNA machinery genes (DROSHA, DICER, AGO1, and GEMIN4) on gastric cancer in Chinese Han population, further revealing the genetic mechanisms of gastric ..
  72. Cikaluk D, Tahbaz N, Hendricks L, DiMattia G, Hansen D, Pilgrim D, et al. GERp95, a membrane-associated protein that belongs to a family of proteins involved in stem cell differentiation. Mol Biol Cell. 1999;10:3357-72 pubmed
    ..The corresponding cDNA was cloned and sequenced and found to encode a protein of 97.6 kDa that we call GERp95 (Golgi ER protein 95 kDa)...
  73. Hauptmann J, Dueck A, Harlander S, Pfaff J, Merkl R, Meister G. Turning catalytically inactive human Argonaute proteins into active slicer enzymes. Nat Struct Mol Biol. 2013;20:814-7 pubmed publisher
    ..Here, we have mutated inactive human Ago1 and Ago3 and generated catalytic Argonaute proteins...
  74. Völler D, Linck L, Bruckmann A, Hauptmann J, Deutzmann R, Meister G, et al. Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. PLoS ONE. 2016;11:e0161165 pubmed publisher
    ..The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity...
  75. Harwig A, Kruize Z, Yang Z, Restle T, Berkhout B. Analysis of AgoshRNA maturation and loading into Ago2. PLoS ONE. 2017;12:e0183269 pubmed publisher
    ..These results suggest the presence of a cellular co-factor involved in AgoshRNA loading into Ago2 in vivo. We also demonstrate specific AgoshRNA loading in Ago2, but not Ago1/3/4, thus further reducing unwanted side effects.
  76. Döll S, Kuhlmann M, Rutten T, Mette M, Scharfenberg S, Petridis A, et al. Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex. Plant J. 2017;: pubmed publisher
    ..Mutants defective in THO, AGO1, SDS3 and RDR6 were impaired with respect to scopolin accumulation in response to stress, suggesting a mechanism ..
  77. Eulalio A, Huntzinger E, Izaurralde E. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Nat Struct Mol Biol. 2008;15:346-53 pubmed publisher
    ..In contrast, silencing is abolished by mutating Argonaute 1 (AGO1) at two conserved phenylalanine residues predicted to mediate binding to the cap structure...
  78. Yoda M, Kawamata T, Paroo Z, Ye X, Iwasaki S, Liu Q, et al. ATP-dependent human RISC assembly pathways. Nat Struct Mol Biol. 2010;17:17-23 pubmed publisher
    ..RNAs (siRNAs) and microRNAs (miRNAs) are incorporated into RISCs containing the Argonaute (AGO) subfamily proteins Ago1-4...
  79. Turchinovich A, Burwinkel B. Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma. RNA Biol. 2012;9:1066-75 pubmed publisher
    ..In this work we compared the total miRNA content in AGO1 and AGO2 immunoprecipitates obtained from MCF7 adenocarcinoma cells using TaqMan Low Density miRNA Arrays and ..
  80. Clark J, Rahman R, Yang N, Yang L, Lau N. Drosophila PAF1 Modulates PIWI/piRNA Silencing Capacity. Curr Biol. 2017;27:2718-2726.e4 pubmed publisher
    ..Our study suggests a mechanistic conservation between fission yeast PAF1 repressing AGO1/small interfering RNA (siRNA)-directed silencing [13, 14] and Drosophila PAF1 opposing PIWI/piRNA-directed ..
  81. Sand M, Skrygan M, Georgas D, Arenz C, Gambichler T, Sand D, et al. Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer. Mol Carcinog. 2012;51:916-22 pubmed publisher
    ..complex subunit DiGeorge syndrome critical region gene 8 (DGCR8) and the RISC components argonaute-1 (AGO1), argonaute-2 (AGO2), as well as double-stranded RNA-binding proteins PACT, TARBP1, and TARBP2 in epithelial skin ..
  82. Schürmann N, Trabuco L, Bender C, Russell R, Grimm D. Molecular dissection of human Argonaute proteins by DNA shuffling. Nat Struct Mol Biol. 2013;20:818-26 pubmed publisher
    ..decipher the complex biology of cellular effectors, exemplified in humans by four pleiotropic Argonaute proteins (Ago1-Ago4)...
  83. Doi N, Zenno S, Ueda R, Ohki Hamazaki H, Ui Tei K, Saigo K. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Curr Biol. 2003;13:41-6 pubmed
    ..Here, we show that Dicer and eIF2C translation initiation factors belonging to the PIWI family (eIF2C1-4) play an essential role in mammalian siRNA-mediated PTGS, most probably through synergistic interactions...
  84. Görücü Yilmaz Ş, Erdal M, Avci Özge A, Sungur M. SNP Variation in MicroRNA Biogenesis Pathway Genes as a New Innovation Strategy for Alzheimer Disease Diagnostics: A Study of 10 Candidate Genes in an Understudied Population From the Eastern Mediterranean. Alzheimer Dis Assoc Disord. 2016;30:203-9 pubmed publisher
    ..104) and allele (P=0.035). Functional SNP variations in the other 8 candidate genes (DGCR8, XPO5, RAN, DICER1, AGO1, AGO2, GEMIN3, and GEMIN4) did not associate with AD in our sample...
  85. Fu X, Dimopoulos G, Zhu J. Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion. Sci Rep. 2017;7:6493 pubmed publisher
    ..We also measured the association of miRNAs with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) to assess the functional status of individual miRNA species...
  86. Chu C, Rana T. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol. 2006;4:e210 pubmed
    ..Here, we show that RCK/p54, a DEAD box helicase, interacts with argonaute proteins, Ago1 and Ago2, in affinity-purified active siRISC or miRISC from human cells; directly interacts with Ago1 and Ago2 in ..
  87. Dachsel J, Taylor J, Mok S, Ross O, Hinkle K, Bailey R, et al. Identification of potential protein interactors of Lrrk2. Parkinsonism Relat Disord. 2007;13:382-5 pubmed
    ..Future investigation of these candidates is now warranted and may help resolve the pathomechanism behind Lrrk2 neurodegeneration. ..
  88. Parisi C, Giorgi C, Batassa E, Braccini L, Maresca G, D Agnano I, et al. Ago1 and Ago2 differentially affect cell proliferation, motility and apoptosis when overexpressed in SH-SY5Y neuroblastoma cells. FEBS Lett. 2011;585:2965-71 pubmed publisher
    ..We have highlighted a novel and non-redundant function of Ago1 versus Ago2; the two core factors of the miRNA-associated RISC complex...
  89. Williams R, Rubin G. ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proc Natl Acad Sci U S A. 2002;99:6889-94 pubmed
    ..We show here that mutations in the Drosophila Argonaute1 (AGO1) gene suppress RNAi in embryos...
  90. Ma K, Li F, Liang P, Chen X, Liu Y, Tang Q, et al. RNA interference of Dicer-1 and Argonaute-1 increasing the sensitivity of Aphis gossypii Glover (Hemiptera: Aphididae) to plant allelochemical. Pestic Biochem Physiol. 2017;138:71-75 pubmed publisher
    ..gossypii to plant allelochemicals. These results should be useful to understand the molecular mechanisms of the cotton aphid adaption to plant allelochemicals. ..
  91. Hu J, Wang F, Zhu X, Yuan Y, Ding M, Gao S. Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing components and its dominant-negative mutant caused two-cell-stage embryonic arrest. Dev Dyn. 2010;239:407-24 pubmed publisher
    ..When ectopically expressed in somatic cells, ZAR1L colocalized with P-body components including EIF2C1(AGO1), EIF2C2(AGO2), DDX6 and LSM14A, and germline-specific chromatoid body components including PIWIL1, PIWIL2, ..
  92. Pradhan M, Pandey P, Gase K, Sharaff M, Singh R, Sethi A, et al. Argonaute 8 (AGO8) Mediates the Elicitation of Direct Defenses against Herbivory. Plant Physiol. 2017;175:927-946 pubmed publisher
    ..transformants (irAGO8) plants but did not differ from the wild type when consuming plants silenced in AGO1 (a, b, and c), AGO2, AGO4 (a and b), AGO7, or AGO10